CN111183929B - Erythroculter ilishaeformis induction pengze crucian gynogenesis method - Google Patents
Erythroculter ilishaeformis induction pengze crucian gynogenesis method Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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Abstract
The invention discloses a method for inducing gynogenesis of carassius auratus gibelio by culter ilishaeformis, which comprises the following steps of screening male and female parents for intensive cultivation, artificially hastening parturition of the female parent of carassius auratus gibelio, artificially inseminating, artificially hatching and cultivating gynogenesis fry; and obtaining the gynogenesis filial generation of the carassius auratus induced by the culter alburnus. The gynogenesis method has strong operability and good adaptability, and in the implementation process, the fertility rate can reach 97.20%, the hatching rate can reach 84.17%, and the survival rate of the fry can reach 86.32%. The morphological characters of filial generation obtained by inducing gynogenesis of the carassius auratus var nitidus by the culter alburnus are biased to female parent, and the weight increase speed of the filial generation group induced by the culter alburnus for gynogenesis of the carassius auratus is 33.29% faster than that of the filial generation group induced by selfing of the carassius auratus.
Description
Technical Field
The invention relates to the technical field of fish gynogenesis, in particular to a method for inducing gynogenesis of carassius auratus through culter alburnus.
Background
Gynogenesis is an important reproduction mode in parthenogenesis of fishes, sperm required by the parthenogenesis is provided by males with similar amphoteric relativity, and the sperm is not fused with eggs after entering the eggs, only plays a role in inducing the development of the eggs, and is a reproduction mode without fusion or pseudo-cooperation[1]. The gynogenesis has very wide application prospect in fish genetic breeding and aquaculture, and is mainly reflected in the aspects of rapid establishment of pure lines, gene-centromere mapping, judgment of sex determination mechanism, utilization of unisexual population and the like[2]。
From Hubbs et al[3]It was found that fish belonging to the family of the flower of Amazon americana (Poecilia formosa) is a naturally occurring gynogenetic population, and it was found that certain types of fish belonging to the genus Poeciopsis and Carassius auratus gibelio (Carassius auratus gibelio) are also gynogenetic populations[4]. Since the 80 s of the 20 th century, China has conducted a great deal of research on gynogenesis fishes and found that Yunchi high back crucian carp[5]Black dragon river silver crucian carp[6]'Henan' ice cream crucian carp[7]Guangdong contracted bone crucian carp[8]Guizhou pran crucian carp[9]Jiangxi Pengzu crucian carp[10]The gynogenesis is performed equally. Examples of heterospermic gynogenesis of carassius auratus pengze are as follows: pengze crucian carp (male parent) and Xingxing carp (male parent)[11]Male parent of carp and pengze carp[13]Male and female carassius auratus Pengze[14]Male and female carp and Pengze carp[15]Male and female carp and Pengze carp[16]And the above are related toNo male parent of Pengze crucian carp nor female parent of Pengze crucian carp.
Carassius auratus (Carassius auratus var. pengsensensis) belongs to Cyprinales, Cyprinaceae, Cyprinidae and Carassius, is native to natural water areas such as Dingjia lake, Taipo lake, Fanghu lake and Yun lake in Pengze county of Jiangxi, and is favored to inhabit reed clusters in the lake. Researchers of the Jiangxi province scientific research institute of aquatic products have carefully bred carassius auratus pengze for 6 successive generations from 1983, and the carassius auratus pengze is elected as a freshwater aquaculture excellent variety by Ministry of agriculture in 1993[17]. The crucian carp in Pengzhou shape has short head, thick back, no need, gray black back, and grey white belly. Under normal conditions, female sexual maturity can be reached in one winter (12 months), while male mature individuals are smaller than females, the breeding season is generally 3-7 months, when the water temperature rises to about 17 ℃, carassius auratus pengze starts to lay eggs and breed, and the breeding peak is at 20-24 ℃. Under natural conditions, the pengze crucian likes running water, especially micro-flowing water with certain turbidity, and spawns and breeds in shallow water areas with dense waterweeds, and the female generally has a fecundity of 4-6 ten thousand[18]. Because the growth speed of the crucian carp is 24.98 percent faster than that of the common crucian carp, and the crucian carp has large individual, has the highest protein content and high edible rate in all the crucian carps, is rich in all 8 necessary amino acids needed by human body, is popular with consumers and is a freshwater aquaculture variety with higher aquaculture potential[19]. The wild resources are reduced due to the damage of natural environment and artificial fishing, and the close reproduction is aggravated due to artificial interference, and the variety is degraded mainly in the characters of disease resistance, growth speed and the like[20]。
Culter alburnus Basilewsky, also known as alburnus, Heshun, Taihu lake whitefish, belongs to Cyprinales, Cyprinidae, Macculter subfamily and Spanish Culter, is a more violent carnivorous fish, is widely distributed in main water systems and auxiliary lakes of Yangtze river basin, and is relatively suitable for living in still water. The age of a breeding group is generally 3-6 years, the laying period is 6-7 months, and adult fishes are clustered in a running water place for breeding; the spawning ground needs to have certain running water, and the fecundity is 3-6 ten thousand generally[21]. It is slender, willow leaf-shaped, flat on the side, and belongs to the middle and upper layersThe large-scale fresh water economic fish acts rapidly and is good for jumping. The culter alburnus is tender in meat quality, delicious in taste, deeply favored by consumers and high in economic value, and is one of famous' Taihu san Bai[22]. With the gradual improvement and popularization of artificial propagation and culture technology, the culture scale of the artificial propagation and culture technology is continuously enlarged, and the artificial propagation and culture technology becomes one of important freshwater economic fishes in China[23]。
The gynogenesis of fishes is an effective means for breeding new species, the filial generation generated by the heterospermic gynogenesis of the pruan crucian carp has the characteristics of high growth speed, good meat quality and flavor, high content of nutrient components, strong disease resistance and adaptability and the like, and meanwhile, the carassia carp heterosis has obvious heterosis in the aspects of food conversion rate, catching rate and low oxygen tolerance, and is convenient for popularization[24]. Research reports that the gynogenesis of the pengze crucian carp and the black longjiang wild carp has the advantages of high growth speed, good meat quality, strong disease resistance, high group yield and the like, the appearance of the pengze crucian carp is similar to that of the crucian carp, the market is good, and the method is easy to popularize[24]. The carassius auratus pengze and the culter alburnus belong to different subfamilies, the carassius auratus pengze and the culter alburnus have great difference in biological characteristics such as heredity, classification, body shape and the like, and no report of success of inducing gynogenesis of carassius auratus pengze by the culter alburnus exists at home and abroad so far, so that an important application value is realized by exploring a method for inducing gynogenesis of the carassius auratus pengze and researching and analyzing the biological characteristics of filial generations of the carassius auratus pen.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides the gynogenesis method of the carassius auratus induced by the culter alburnus.
In order to achieve the purpose, the invention designs a method for inducing gynogenesis of carassius auratus var bellatus, which comprises the following steps:
1) screening male and female parents and carrying out reinforced cultivation:
the male parent is selected from the sexually mature culter alburnus (the origin is the Danjiang mouth reservoir), the female parent is selected from the sexually mature Pengze crucian (purchased from Jiangxi province institute of aquatic science), and the male and female parents are respectively subjected to parent reinforced cultivation before delivery;
2) artificial induction of female carassius auratus pengze parents: selecting middle ten days of May, injecting oxytocic into female parent of Carassius pengzensis by two-time injection method, and injecting luteinizing hormone releasing hormone analogue (LRH-A) into the first injection2) Injecting the compound agent by a second needle, wherein the time interval between two times of injection is 8-10 h, and putting the pengze crucian carp after injection into an oxytocic pool; wherein, in the first needle injection, the luteinizing hormone releasing hormone analogue (LRH-A)2) The injection amount is 0.5-3.0 mug/kg;
in the second needle injection, the compound medicament is a combined medicament of luteinizing hormone releasing hormone analogue (LRH-A2) and Diosone (DOM), and the injection amount of the compound medicament is 2-8 mu g/kg and 2-8 mg/kg respectively;
3) artificial spawning induction of male parent of culter alburnus: when the crucian carp female parent is injected with a second needle, the topromotein releasing hormone analogue (LRH-A) is injected into the male parent of the culter alburnus for oxytocic injection2) Wherein the luteinizing hormone-releasing hormone analogue injection amount (LRH-A)2) 3-5 mug/kg;
4) artificial insemination: squeezing eggs of the carassius auratus pengze into a basin, then squeezing sperms of the culter alburnus, and then slightly shaking the basin to promote the sperms of the culter alburnus to induce gynogenesis of the eggs of the carassius auratus pengze;
5) artificial incubation: putting the gynogenesis fertilized eggs into an incubation barrel for incubation in running water, and incubating fish fries;
6) breeding gynogenesis fish fries: and when the fries swim smoothly, moving the fries out of the incubation barrel, putting the fries into a culture pond for fry culture to obtain gynogenesis filial generations of the carassius auratus induced by the culter alburnus, and culturing the gynogenesis filial generations according to the feed and the culture mode of the carassius auratus.
Further, in the step 2), the injection amount of the luteinizing hormone releasing hormone analogue (LRH-A2) in the first needle injection is 1 mug/kg.
Still further, in the step 2), the luteinizing hormone-releasing hormone analogue (LRH-A) is injected in the second needle2) The injection amount is 4 mug/kg; the injection amount of Deovolone (DOM) was 4 mg/kg.
Still further, in the step 3)Luteinizing hormone releasing hormone analog (LRH-A)2) The dose of (2) is 4. mu.g/kg.
Further, in the step 5), water is fed into the bottom of the hatching barrel, water overflows from the upper part of the hatching barrel, a 60-mesh window gauze is arranged at an overflow port (preventing fertilized eggs and newly hatched fries from flowing out with water), and during hatching, the fertilized eggs are kept in a suspension state in a hatching pond, the hatching temperature is 26 ℃, and the hatching time is 58 hours.
Still further, in the step 6), the breeding of the fry comprises a first-stage breeding and a second-stage breeding, wherein,
first-stage cultivation: feeding the fries with twigs in a cement pond, carrying out secondary cultivation when the fries grow to 2-3cm,
secondary cultivation: and (4) transferring the fish fries into an earth pond, and culturing according to the commonly used fish fry culturing mode to obtain gynogenesis filial generations of the carassius auratus induced by the culter alburnus.
The invention has the beneficial effects that:
the carassius auratus pengze and the culter alburnus selected by the invention belong to different subfamilies of fish, and have larger difference in biological characteristics such as heredity, classification, body shape and the like, so that no report about successful development of the carassius auratus pengze through induction by the culter alburnus is provided at home and abroad. The invention carries out growth comparison experiments on gynogenesis progeny population of the carassius auratus var pengze induced by the culter alburnus and the selfing progeny population of the carassius auratus pengze, and the result shows that the average body length of the selfing progeny population of the carassius auratus pengze is as follows: 9.0616 +/-0.761 mm, and the average body mass is 25.037 +/-5.010 g; the average growth of gynogenesis filial population of carassius auratus induced by the culter alburnus is as follows: 9.9434 +/-0.847 mm, the average body mass is 33.372 +/-9.553 g, and the weight growth speed of the filial generation group induced by the culter alburnus and gynogenesis of the carassius auratus is 33.29% faster than that of the filial generation group induced by the carassius auratus.
The invention obtains the absolute weight gain rate and the absolute weight gain rate of two combined offspring by measuring the length and the weight of 30-150 days old Erythroculter ilishaeformis gynogenesis offspring groups and the pengze crucian inbred offspring groups, and is shown in figure 1 and figure 2, and the results show that: starting from the age of 90 days, the absolute weight gain rate of the gynogenesis filial population of the carassius auratus var ilishaeformis induced by the culter ilishaeformis is obviously greater than that of the carassius auratus inbred filial population, and in addition, the phenomenon that the gynogenesis filial population of the carassius auratus induced by the culter ilishaeformis exhibits high ingestion speed is observed in the feeding process, so that the gynogenesis of the carassius auratus induced by the culter ilishaeformis is proved to obtain a filial generation with high ingestion capability and rapid growth.
The gynogenesis method has strong operability and good adaptability, and in the implementation process, the fertility rate can reach 97.20%, the hatching rate can reach 84.17%, and the survival rate of the fry can reach 86.32%. The characters of filial generation obtained by inducing gynogenesis of the carassius auratus var pengze by the culter alburnus are biased to female parent, and the weight increase speed of the filial generation of the gynogenesis of the carassius auratus induced by the culter alburnus is 33.29% faster than that of the filial generation of the carassius auratus inbred.
Drawings
FIG. 1 is a schematic diagram of the absolute growth rate of different days of carassius auratus (P-Q) filial generations induced by carassius auratus inbred (P-P) and culter ilishaeformis;
fig. 2 is a schematic diagram of the absolute weight gain rate of different days of the inbred filial generation (P-P) of carassius auratus Pengze and the carassius auratus (P-Q) induced by the culter alburnus.
Detailed Description
The present invention is described in further detail below with reference to specific examples so as to be understood by those skilled in the art.
Example 1
The method for inducing gynogenesis of carassius auratus var bellatus comprises the following steps:
1) the mature culter alburnus parent is from the reservoir at the Yangjiang river mouth, and the collection specification is 2 kg/tail; the female parent of the Pengze crucian comes from the Pengze crucian with sexual maturity provided by Jiangxi province water producing science research institute, and the parent intensive cultivation is respectively carried out before the production.
2) Artificial induction of female carassius auratus pengze parents: selecting middle ten days of May, injecting oxytocic into female parent of Carassius pengzensis by two-time injection method, and injecting luteinizing hormone releasing hormone analogue (LRH-A) into the first injection2) Injecting the compound agent by a second needle, wherein the time interval between two times of injection is 8-10 h, and putting the pengze crucian carp after injection into an oxytocic pool; wherein, in the first needle injection, the injection amount of the luteinizing hormone releasing hormone analogue is (LRH-A)2) 1.0 mug/kg;
second needleIn injection, the compound preparation is luteinizing hormone releasing hormone analog (LRH-A)2) And Deoxolone (DOM) in an amount of 4 μ g/kg and 4mg/kg, respectively;
3) artificial spawning induction of male parent of culter alburnus: when the crucian carp female parent is injected with a second needle, the topromotein releasing hormone analogue (LRH-A) is injected into the male parent of the culter alburnus for oxytocic injection2) Wherein the luteinizing hormone-releasing hormone analogue injection amount (LRH-A)2) 4 mug/kg;
4) artificial insemination: squeezing eggs of the carassius auratus pengze into a basin, then squeezing sperms of the culter alburnus, and then slightly shaking the basin to promote the sperms of the culter alburnus to induce gynogenesis of the eggs of the carassius auratus pengze;
5) artificial incubation: putting the gynogenesis fertilized eggs into an incubation barrel for incubation in running water, and incubating fish fries;
6) breeding gynogenesis fish fries: and when the fry swim smoothly, the fry is moved out of the hatching barrel and is placed into a culture pond for fry culture. The method comprises the following steps of culturing fries, wherein the first-stage culturing is carried out in a cement pond, feeding the fries with twigs, and culturing the fries in a second-stage manner when the fries are 2-3cm in length, namely, the fries are transplanted into an earthen pond and cultured according to a conventional fry culturing mode commonly used in the prior art to obtain the gynogenesis filial generation of the carassius auratus induced by the culter alburnus. Culturing gynogenesis filial generation in the soil pond according to the feed and the culture mode of the carassius auratus pengze.
In this example, the quantitative and countable characteristics of pengze crucian carp, culter alburnus and gynogenesis progeny between the pengze crucian carp and the culter alburnus are counted (as shown in table 1), and the result shows that the characteristics of the gynogenesis progeny are basically similar to the pengze crucian carp. In order to further explore the character characteristics of gynogenesis filial generation, the experiment measures the countability and the quantifiability of 60 gynogenesis filial generation and Pengze crucian carp, and comparing the two properties (as shown in Table 2), the gynogenesis offspring and Pengze crucian have no significant difference in the quantitative proportion properties such as full length/body length, body height/body length, body thickness/body length, head length/body length, tail handle height/body length, tail handle length/tail handle height, kiss length/body length, eye diameter/head length, inter-ocular distance/head length, etc., the gynogenesis offspring has no difference from Pengze crucian in the number of dorsal fin spines, the number of hip fin spines, the number of pectoral fin fins, the scales on the side line, the scales under the side line, the number of gills and rakes and the like, and the characteristics of the first filial generation of the gynogenesis of the Pengze crucian induced by the culter alburnus are more favorable than that of the Pengze crucian.
In order to investigate whether the growth characteristics of gynogenesis progeny of carassius auratus var pusillus induced by culter alburnus and the growth characteristics of inbred progeny of carassius auratus, the embodiment performs a growth comparison experiment on the two combined progeny, that is, when the progeny generated by the two combinations are in a horizontal trip, 1500 tails of the two combinations are selected to be transferred into the carassius auratus with the volume of about 1m3The breeding is carried out in a round white barrel, 500 tails are placed in each barrel, 3 parallel controls are combined for each barrel, the same-proportion breeding test and the standardized breeding are carried out, namely, each barrel is fed with food regularly, at fixed points and quantitatively every day, and the pollution is absorbed regularly, so that the offspring breeding conditions of each experimental group are ensured to be consistent. And measuring the body length and the body mass of each combined offspring 5 times in total (20 fishes are randomly selected from each breeding barrel every time) at 30 days old, 60 days old, 90 days old, 120 days old, 150 days old and the like, and randomly taking out the same number of fishes from each breeding barrel after measurement so as to reduce the breeding density.
TABLE 1 statistics of quantitative and countable traits of inbred filial generations of Carassius pengzensis and induced carassius auratus kurz
TABLE 2 analysis of the mean, standard deviation and variance of the characteristics of selfed offspring of Carassius pengzensis and the offspring of Carassius auratus poensis induced by Erythroculter ilishaeformis
Example 2
The method for inducing gynogenesis of carassius auratus var bellatus comprises the following steps:
1) the mature culter alburnus parent is from the reservoir at the Yangjiang river mouth, and the collection specification is 2 kg/tail; the female parent of the Pengze crucian comes from the Pengze crucian with sexual maturity provided by Jiangxi province water producing science research institute, and the parent intensive cultivation is respectively carried out before the production.
2) Artificial induction of female carassius auratus pengze parents: selecting middle ten days of May, injecting oxytocic into female parent of Carassius pengzensis by two-time injection method, and injecting luteinizing hormone releasing hormone analogue (LRH-A) into the first injection2) Injecting the compound agent by a second needle, wherein the time interval between two times of injection is 8-10 h, and putting the pengze crucian carp after injection into an oxytocic pool; wherein, in the first needle injection, the injection amount of the luteinizing hormone releasing hormone analogue is (LRH-A)2) 3.0 mug/kg;
in the second injection, the compound preparation is luteinizing hormone releasing hormone analog (LRH-A)2) And Deoxolone (DOM) in an amount of 2 μ g/kg and 8mg/kg, respectively;
3) artificial spawning induction of male parent of culter alburnus: when the crucian carp female parent is injected with a second needle, the topromotein releasing hormone analogue (LRH-A) is injected into the male parent of the culter alburnus for oxytocic injection2) Wherein the luteinizing hormone-releasing hormone analogue injection amount (LRH-A)2) 3 mug/kg;
4) artificial insemination: squeezing eggs of the carassius auratus pengze into a basin, then squeezing sperms of the culter alburnus, and then slightly shaking the basin to promote the sperms of the culter alburnus to induce gynogenesis of the eggs of the carassius auratus pengze;
5) artificial incubation: putting the gynogenesis fertilized eggs into an incubation barrel for incubation in running water, and incubating fish fries;
6) breeding gynogenesis fish fries: and when the fry swim smoothly, the fry is moved out of the hatching barrel and is placed into a culture pond for fry culture. The method comprises the following steps of culturing fries, wherein the first-stage culturing is carried out in a cement pond, feeding the fries with twigs, and culturing the fries in a second-stage manner when the fries are 2-3cm in length, namely, the fries are transplanted into an earthen pond and cultured according to a conventional fry culturing mode commonly used in the prior art to obtain the gynogenesis filial generation of the carassius auratus induced by the culter alburnus. Culturing gynogenesis filial generation in the soil pond according to the feed and the culture mode of the carassius auratus pengze.
Example 3
The method for inducing gynogenesis of carassius auratus var bellatus comprises the following steps:
1) the mature culter alburnus parent is from the reservoir at the Yangjiang river mouth, and the collection specification is 2 kg/tail; the female parent of the Pengze crucian comes from the Pengze crucian with sexual maturity provided by Jiangxi province water producing science research institute, and the parent intensive cultivation is respectively carried out before the production.
2) Artificial induction of female carassius auratus pengze parents: selecting middle ten days of May, injecting oxytocic into female parent of Carassius pengzensis by two-time injection method, and injecting luteinizing hormone releasing hormone analogue (LRH-A) into the first injection2) Injecting the compound agent by a second needle, wherein the time interval between two times of injection is 8-10 h, and putting the pengze crucian carp after injection into an oxytocic pool; wherein, in the first needle injection, the injection amount of the luteinizing hormone releasing hormone analogue is (LRH-A)2) 5 mug/kg;
in the second injection, the compound preparation is luteinizing hormone releasing hormone analog (LRH-A)2) And Deoxolone (DOM) in an amount of 8 μ g/kg and 2mg/kg, respectively;
3) artificial spawning induction of male parent of culter alburnus: when the crucian carp female parent is injected with a second needle, the topromotein releasing hormone analogue (LRH-A) is injected into the male parent of the culter alburnus for oxytocic injection2) Wherein the luteinizing hormone-releasing hormone analogue injection amount (LRH-A)2) 3-5 mug/kg;
4) artificial insemination: squeezing eggs of the carassius auratus pengze into a basin, then squeezing sperms of the culter alburnus, and then slightly shaking the basin to promote the sperms of the culter alburnus to induce gynogenesis of the eggs of the carassius auratus pengze;
5) artificial incubation: putting the gynogenesis fertilized eggs into an incubation barrel for incubation in running water, and incubating fish fries;
6) breeding gynogenesis fish fries: and when the fry swim smoothly, the fry is moved out of the hatching barrel and is placed into a culture pond for fry culture. The method comprises the following steps of culturing fries, wherein the first-stage culturing is carried out in a cement pond, feeding the fries with twigs, and culturing the fries in a second-stage manner when the fries are 2-3cm in length, namely, the fries are transplanted into an earthen pond and cultured according to a conventional fry culturing mode commonly used in the prior art to obtain the gynogenesis filial generation of the carassius auratus induced by the culter alburnus. Culturing gynogenesis filial generation in the soil pond according to the feed and the culture mode of the carassius auratus pengze.
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[22] Bao, Zhao Ji Wei, Dong Chong Zhi, etc. the growth characteristics of the Erythroculter ilishaeformis in Xingkai lake [ J ] ocean and lake marsh 2005,36(2): 146-.
[23] Li faithful, ran, Yanma, and others the reproduction biological characteristics of the Yangjiang culter [ J ]. Anima J.Anzoology, 2017,2: 263-plus 270.
[24] Picrass, Anmian, Fangshi, etc. Puan silver crucian carp heterospermic gynogenesis offspring rice field breeding test [ J ] scientific fish breeding, 2012,2:57-59.
[25] The cross-breeding research of Pengze crucian carp (male parent) and Heilongjiang wild carp (male parent) is [ J ]. the journal of aquatics, 2004,17(1):14-20.
Other parts not described in detail are prior art. Although the present invention has been described in detail with reference to the above embodiments, it is only a part of the embodiments of the present invention, not all of the embodiments, and other embodiments can be obtained without inventive step according to the embodiments, and the embodiments are within the scope of the present invention.
Claims (6)
1. A gynogenesis method of carassius auratus induced by culter alburnus is characterized in that: the method comprises the following steps:
1) screening male and female parents and carrying out reinforced cultivation:
the male parent is selected from the mature Erythroculter ilishaeformis of the Yangjiang estuary reservoir, the female parent is selected from the mature Pengze crucian carp, and the male and female parents are respectively subjected to enhanced cultivation before delivery;
2) artificial induction of female carassius auratus pengze parents: selecting middle ten-month-May, injecting an oxytocic into female carassius auratus pazewalskii parents by adopting a two-time injection method, injecting a luteinizing hormone releasing hormone analogue into a first needle, injecting a compound medicament into a second needle, wherein the time interval between two-time injection is 8-10 hours, and putting the carassius pazewalskii which is subjected to injection into an oxytocic pool; wherein, in the first needle injection, the injection amount of the luteinizing hormone releasing hormone analogue is 0.5-3.0 mu g/kg; in the second needle injection, the compound medicament is a combined medicament of luteinizing hormone releasing hormone analogue and diosone, and the injection amount of the compound medicament is 2-8 mu g/kg and 2-8 mg/kg respectively;
3) artificial spawning induction of male parent of culter alburnus: when the crucian carp male parent is injected with a second needle, injecting a luteinizing hormone releasing hormone analogue into the induced spawning of the culter alburnus male parent, wherein the injection amount of the luteinizing hormone releasing hormone analogue is 3-5 mu g/kg;
4) artificial insemination: squeezing eggs of the carassius auratus pengze into a basin, then squeezing sperms of the culter alburnus, and then slightly shaking the basin to promote the sperms of the culter alburnus to induce gynogenesis of the eggs of the carassius auratus pengze;
5) artificial incubation: putting the gynogenesis fertilized eggs into an incubation barrel for incubation in running water, and incubating fish fries;
6) breeding gynogenesis fish fries: and when the fries swim smoothly, moving the fries out of the incubation barrel, putting the fries into a culture pond for fry culture to obtain gynogenesis filial generations of the carassius auratus induced by the culter alburnus, and culturing the gynogenesis filial generations according to the feed and the culture mode of the carassius auratus.
2. The method for inducing gynogenesis of carassius auratus gibelio by the culter ilishaeformis as claimed in claim 1, which is characterized in that: in the step 2), the injection amount of the luteinizing hormone releasing hormone analogue in the first injection is 1 mug/kg.
3. The method for inducing gynogenesis of carassius auratus gibelio by the culter ilishaeformis as claimed in claim 1, which is characterized in that: in the step 2), in the second needle injection, the injection amount of the luteinizing hormone releasing hormone analogue is 4 mug/kg; the injection amount of the diutanone is 4 mg/kg.
4. The method for inducing gynogenesis of carassius auratus gibelio by the culter ilishaeformis as claimed in claim 1, which is characterized in that: in the step 3), the dosage of the luteinizing hormone releasing hormone analogue is 4 mug/kg.
5. The method for inducing gynogenesis of carassius auratus gibelio by the culter ilishaeformis as claimed in claim 1, which is characterized in that: in the step 5), water is fed into the bottom of the hatching barrel, water overflows from the upper part of the hatching barrel, a 60-mesh window gauze is arranged on the water overflow port, and during hatching, the fertilized eggs keep a suspension state in a hatching pond, the hatching temperature is 26 ℃, and the hatching time is 58 hours.
6. The method for inducing gynogenesis of carassius auratus gibelio by the culter ilishaeformis as claimed in claim 1, which is characterized in that: in the step 6), the fry breeding comprises first-stage breeding and second-stage breeding, wherein,
first-stage cultivation: feeding the fries with twigs in a cement pond, carrying out secondary cultivation when the fries grow to 2-3cm,
secondary cultivation: and (4) transferring the fish fries into an earth pond, and culturing according to the commonly used fish fry culturing mode to obtain the gynogenesis filial generation of the carassius auratus induced by the culter alburnus.
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