CN106577388B - A hybrid breeding method of Huangyou No. 1 peltrelative catfish with improved growth traits - Google Patents
A hybrid breeding method of Huangyou No. 1 peltrelative catfish with improved growth traits Download PDFInfo
- Publication number
- CN106577388B CN106577388B CN201611154851.7A CN201611154851A CN106577388B CN 106577388 B CN106577388 B CN 106577388B CN 201611154851 A CN201611154851 A CN 201611154851A CN 106577388 B CN106577388 B CN 106577388B
- Authority
- CN
- China
- Prior art keywords
- hybrid
- parent
- yellow catfish
- male
- female parent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000009395 breeding Methods 0.000 title claims description 55
- 241001233037 catfish Species 0.000 title claims description 9
- 241000376029 Tachysurus fulvidraco Species 0.000 claims abstract description 227
- 238000012216 screening Methods 0.000 claims abstract description 20
- 238000011534 incubation Methods 0.000 claims abstract description 12
- 238000009396 hybridization Methods 0.000 claims abstract description 3
- 241000251468 Actinopterygii Species 0.000 claims description 100
- 235000013601 eggs Nutrition 0.000 claims description 82
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 59
- 230000001488 breeding effect Effects 0.000 claims description 47
- 229940088597 hormone Drugs 0.000 claims description 45
- 239000005556 hormone Substances 0.000 claims description 45
- XNOPRXBHLZRZKH-UHFFFAOYSA-N Oxytocin Natural products N1C(=O)C(N)CSSCC(C(=O)N2C(CCC2)C(=O)NC(CC(C)C)C(=O)NCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(CCC(N)=O)NC(=O)C(C(C)CC)NC(=O)C1CC1=CC=C(O)C=C1 XNOPRXBHLZRZKH-UHFFFAOYSA-N 0.000 claims description 34
- 101800000989 Oxytocin Proteins 0.000 claims description 34
- 102100031951 Oxytocin-neurophysin 1 Human genes 0.000 claims description 34
- XNOPRXBHLZRZKH-DSZYJQQASA-N oxytocin Chemical compound C([C@H]1C(=O)N[C@H](C(N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CSSC[C@H](N)C(=O)N1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(C)C)C(=O)NCC(N)=O)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 XNOPRXBHLZRZKH-DSZYJQQASA-N 0.000 claims description 34
- 229960001723 oxytocin Drugs 0.000 claims description 34
- 102000011022 Chorionic Gonadotropin Human genes 0.000 claims description 30
- 108010062540 Chorionic Gonadotropin Proteins 0.000 claims description 30
- 229940015047 chorionic gonadotropin Drugs 0.000 claims description 30
- 108700012941 GNRH1 Proteins 0.000 claims description 27
- 239000000579 Gonadotropin-Releasing Hormone Substances 0.000 claims description 27
- 150000001875 compounds Chemical class 0.000 claims description 24
- 230000037396 body weight Effects 0.000 claims description 22
- 230000012447 hatching Effects 0.000 claims description 19
- 210000002149 gonad Anatomy 0.000 claims description 16
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 claims description 15
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 claims description 15
- 210000002969 egg yolk Anatomy 0.000 claims description 13
- 230000006378 damage Effects 0.000 claims description 12
- 238000011161 development Methods 0.000 claims description 11
- 230000018109 developmental process Effects 0.000 claims description 11
- 230000001568 sexual effect Effects 0.000 claims description 11
- 239000004568 cement Substances 0.000 claims description 10
- 238000000605 extraction Methods 0.000 claims description 10
- 210000004907 gland Anatomy 0.000 claims description 10
- 230000026109 gonad development Effects 0.000 claims description 10
- 239000007924 injection Substances 0.000 claims description 10
- 238000002347 injection Methods 0.000 claims description 10
- 230000006698 induction Effects 0.000 claims description 7
- 210000000006 pectoral fin Anatomy 0.000 claims description 6
- 206010021718 Induced labour Diseases 0.000 claims description 5
- 230000003187 abdominal effect Effects 0.000 claims description 5
- 230000004720 fertilization Effects 0.000 claims description 5
- 230000009027 insemination Effects 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 238000010397 one-hybrid screening Methods 0.000 claims description 5
- 230000016087 ovulation Effects 0.000 claims description 5
- 210000000582 semen Anatomy 0.000 claims description 5
- 229910001220 stainless steel Inorganic materials 0.000 claims description 5
- 239000010935 stainless steel Substances 0.000 claims description 5
- 238000012546 transfer Methods 0.000 claims description 5
- 238000007689 inspection Methods 0.000 claims description 4
- 238000005070 sampling Methods 0.000 claims description 4
- HHUZGDMRRLQZIQ-PXWUZWBYSA-N 3alpha,6alpha-Dihydroxy-5beta-pregnan-20-one Chemical compound C([C@H]1[C@@H](O)C2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H](C(=O)C)[C@@]2(C)CC1 HHUZGDMRRLQZIQ-PXWUZWBYSA-N 0.000 claims description 2
- 102000006771 Gonadotropins Human genes 0.000 claims description 2
- 108010086677 Gonadotropins Proteins 0.000 claims description 2
- OFOBLEOULBTSOW-UHFFFAOYSA-N Propanedioic acid Natural products OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 claims description 2
- 239000002622 gonadotropin Substances 0.000 claims description 2
- 239000011976 maleic acid Substances 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 9
- 241001471302 Tachysurus Species 0.000 abstract description 5
- 238000009402 cross-breeding Methods 0.000 abstract description 3
- 230000008774 maternal effect Effects 0.000 abstract 1
- 230000002354 daily effect Effects 0.000 description 14
- 230000002924 anti-infective effect Effects 0.000 description 12
- 208000027418 Wounds and injury Diseases 0.000 description 11
- 208000014674 injury Diseases 0.000 description 11
- 238000002474 experimental method Methods 0.000 description 9
- 230000001580 bacterial effect Effects 0.000 description 7
- 241000607528 Aeromonas hydrophila Species 0.000 description 6
- 230000008901 benefit Effects 0.000 description 6
- 238000011156 evaluation Methods 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 230000004083 survival effect Effects 0.000 description 6
- 208000035240 Disease Resistance Diseases 0.000 description 5
- 102000002322 Egg Proteins Human genes 0.000 description 5
- 108010000912 Egg Proteins Proteins 0.000 description 5
- 238000009360 aquaculture Methods 0.000 description 5
- 244000144974 aquaculture Species 0.000 description 5
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 235000013345 egg yolk Nutrition 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 230000037406 food intake Effects 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 206010052428 Wound Diseases 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 230000003203 everyday effect Effects 0.000 description 3
- 239000011152 fibreglass Substances 0.000 description 3
- 239000003365 glass fiber Substances 0.000 description 3
- 239000002434 gonadorelin derivative Substances 0.000 description 3
- 238000003306 harvesting Methods 0.000 description 3
- 238000009400 out breeding Methods 0.000 description 3
- 230000001717 pathogenic effect Effects 0.000 description 3
- 239000002504 physiological saline solution Substances 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 230000008733 trauma Effects 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- 235000019786 weight gain Nutrition 0.000 description 3
- 241001441703 Bagridae Species 0.000 description 2
- 241000598137 Pedunculus Species 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 210000004197 pelvis Anatomy 0.000 description 2
- 230000033458 reproduction Effects 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 208000004232 Enteritis Diseases 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 230000002559 cytogenic effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000019138 food restriction Nutrition 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000009399 inbreeding Methods 0.000 description 1
- 238000009403 interspecific hybridization Methods 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
- Fodder In General (AREA)
Abstract
Description
技术领域technical field
本发明属于水产养殖技术领域，具体涉及一种改良生长性状的黄优1号黄颡鱼的杂交繁育方法，是一种利用鱼类种间杂交育种技术、亲本筛选和培育创新技术产生具有生长快速、抗逆和抗病优势的新品种黄优1号黄颡鱼。The invention belongs to the technical field of aquaculture, and in particular relates to a method for hybrid breeding of Huangyou No. 1 yellow catfish with improved growth characteristics, which is a method for producing fast-growing yellow catfish using interspecies hybrid breeding technology, parental screening and innovative breeding technology. Huangyou No. 1, a new species of yellow catfish with the advantages of stress resistance and disease resistance.
背景技术Background technique
黄颡鱼Pelteobagrus fulvidraco(Richardson)隶属鲇形目(Sliuriformes)、鲿科(Bagridae)、黄颡鱼属(Pelteobagrus)，俗名黄姑鱼，黄嘎，黄腊丁等。瓦氏黄颡鱼Pelteobagrus vachehi(Richardson)隶属鲇形目(Sliuriformes)、鲿科(Bagridae)、黄颡鱼属(Pelteobagrus)，俗名郎丝、黄腊丁、江颡等。黄颡鱼Pelteobagrus fulvidraco和瓦氏黄颡鱼Pelteobagrus vachehi为同属鱼类，在长江水系广为分布，是我国较重要的底层小型经济鱼类。黄颡鱼适于在池塘中养殖，但是它个体较小，生长速度较慢，瓦氏黄颡鱼个体大，生长迅速，但它耐低氧能力和抗逆性较差，较难适于池塘养殖。此外，瓦氏黄颡鱼大多体色呈灰白色，在大部分地区的市场消费习惯上不容易接收。Pelteobagrus fulvidraco (Richardson) belongs to Sliuriformes, Bagridae, and Pelteobagrus. Pelteobagrus vachehi (Richardson) belongs to Sliuriformes, Bagridae, and Pelteobagrus, and its common names are Langsi, Huanglading, Jiangyan and so on. Pelteobagrus fulvidraco and Pelteobagrus vachehi belong to the same genus. They are widely distributed in the Yangtze River system and are important small demersal economic fishes in my country. Peeled catfish is suitable for farming in ponds, but its individuality is small and its growth rate is slow. Peeled catfish is large and its growth rate is fast, but its low oxygen resistance and stress resistance are poor, so it is difficult to adapt to ponds. breeding. In addition, most of the body color of Washii's yellow catfish is off-white, which is not easy to accept in the market consumption habits of most regions.
近几年，国内黄颡鱼成鱼养殖产量已超过40万吨，年产值80多亿元。但是随着黄颡鱼养殖业的快速发展，一些问题相继出现，例如，近亲交配现象严重，导致黄颡鱼种质退化、生长速度降低、抗病力下降，病害发生日趋严重。在苗种培育阶段寄生虫、细菌性烂鳃、腐皮和肠炎等疾病严重影响了黄颡鱼苗种的正常培育，使黄颡鱼苗种养成率较低。每年由于病害造成黄颡鱼养殖业的经济损失惨重，严重影响了黄颡鱼的养殖推广。生长变慢以及病害频发是限制我国黄颡鱼养殖业可持续性发展的瓶颈因子，也影响了黄颡鱼在沿海省市的进一步推广和养殖。因此，黄颡鱼养殖业的发展迫切需要培育生长快速、抗逆性和抗病性强的优良新品种。In recent years, the domestic production of adult yellow catfish has exceeded 400,000 tons, with an annual output value of more than 8 billion yuan. However, with the rapid development of the peltaceae aquaculture industry, some problems have emerged one after another. For example, the phenomenon of inbreeding is serious, which leads to the germplasm degradation, slow growth rate, and decline of disease resistance of peltrow catfish, and the occurrence of diseases is becoming more and more serious. Diseases such as parasites, bacterial gill rot, rotting skin, and enteritis have seriously affected the normal cultivation of peliotius peltatus seedlings during the seedling cultivation stage, making the growth rate of pelttail catfish seedlings low. Every year, the economic loss of the yellow catfish aquaculture industry is heavy due to the disease, which seriously affects the cultivation and promotion of the yellow catfish. Slow growth and frequent disease are the bottleneck factors that limit the sustainable development of my country's yellow catfish aquaculture industry, and also affect the further promotion and cultivation of yellow catfish in coastal provinces and cities. Therefore, the development of the yellow catfish aquaculture industry is in urgent need of cultivating fast-growing, stress-resistant and disease-resistant fine new varieties.
杂交是指不同种类之间或同一种类的不同地理群体之间进行交配培育后代的方法。由于两个亲本在遗传距离上相差较远，因而杂交的后代，即杂交子一代在生长性能和抗病害等方面通常优于双亲，这种现象称为杂交优势。杂交育种是作物遗传育种中最重要的方法之一，迄今为止，在一些粮食作物中已通过不同种类杂交培育出一些在生长性能上具有明显优势的杂交品种，例如杂交水稻和玉米等。国内近几年在黄颡鱼遗传育种研究方面，主要开展了细胞遗传工程和鱼类性别控制育种技术(刘汉勤等2007；徐辉，2007；陈荣国等，2013)。但有关筛选和培育生长和抗逆性强的黄颡鱼和瓦氏黄颡鱼进行杂交培育生长和抗病力强的优良新品种，目前尚未见报道。Hybridization refers to the method of breeding offspring between different species or between different geographical groups of the same species. Because the two parents are far apart in genetic distance, the offspring of the hybrid, that is, the first generation of hybrids, is usually better than the parents in terms of growth performance and disease resistance. This phenomenon is called hybrid vigor. Hybrid breeding is one of the most important methods in crop genetics and breeding. So far, in some food crops, some hybrid varieties with obvious advantages in growth performance have been bred by crossing different types, such as hybrid rice and corn. In recent years, domestic research on the genetics and breeding of yellow catfish has mainly carried out cytogenetic engineering and fish sex control breeding technology (Liu Hanqin et al. 2007; Xu Hui, 2007; Chen Rongguo et al., 2013). However, there is no report yet about screening and cultivating the excellent new varieties of yellow catfish and yellow catfish with strong growth and stress resistance and crossbreeding growth and disease resistance.
发明内容Contents of the invention
为解决上述现有技术存在的问题，本发明提供了一种改良生长性状的黄优1号黄颡鱼的杂交繁育方法，该方法培育出了黄颡鱼♀与瓦氏黄颡鱼♂杂交繁育的杂交一代F1黄优1号，其体色为黄褐色，且生长优势极为明显，抗逆性强。In order to solve the above-mentioned problems in the prior art, the present invention provides a hybrid breeding method of Huangyou No. 1 yellow catfish with improved growth characteristics. The method breeds yellow catfish ♀ and yellow catfish ♂ for cross breeding The first-generation hybrid F1 Huangyou 1 has a yellowish-brown body color, an extremely obvious growth advantage, and strong stress resistance.
实现本发明上述目的所采用的技术方案为：The technical scheme adopted to realize the above-mentioned purpose of the present invention is:
一种改良生长性状的黄优1号黄颡鱼的杂交繁育方法，包括如下步骤：A method for hybrid breeding of Huangyou No. 1 yellow catfish with improved growth characteristics, comprising the steps of:
1、快速生长杂交亲本的筛选：1. Screening of fast-growing hybrid parents:
1.1、杂交母本的筛选：1.1. Screening of hybrid female parents:
头年4月中旬至11月底，选择体表无伤、体重在100g以上的野生雌性黄颡鱼作为初始黄颡鱼母本；选择体表无伤、体重在250g以上的野生雄性黄颡鱼作为初始黄颡鱼父本，初始黄颡鱼母本和初始黄颡鱼父本选择好后，在人工养殖条件下，经驯食能摄食人工配合饲料后进行初始黄颡鱼亲本培育，年后5-7月份，观察初始黄颡鱼亲鱼的性腺发育程度，选择发育成熟的初始黄颡鱼亲本进行繁殖催产、孵化以及鱼种培育，在黄颡鱼苗种快速生长关键阶段，即当年的7-9月份，选择生长快、抗逆能力强的个体，选留4-6％的个体进行培育，第二年或第三年达到性成熟，选择雌性作为快速生长杂交黄颡鱼母本选育系，简称杂交母本；From mid-April to the end of November of the first year, wild female yellow catfish with no injuries on the body surface and a weight of more than 100 g were selected as the initial female parent; wild male yellow catfish with no injuries on the body surface and a weight of more than 250 g were selected as After the initial yellow catfish male parent, the initial yellow catfish female parent and the initial yellow catfish male parent are selected, under artificial breeding conditions, the initial yellow catfish parent can be cultivated after being able to ingest artificial compound feed through domestication. After 5 years -In July, observe the gonad development degree of the initial yellow catfish broodstock, and select the mature initial yellow catfish parent to reproduce, induce spawning, hatch and fish fingerlings. During the critical stage of rapid growth of yellow catfish fry, that is, 7-9 of the year In the first month, select individuals with fast growth and strong stress resistance, select and retain 4-6% of the individuals for cultivation, and reach sexual maturity in the second or third year, and select females as the fast-growing hybrid yellow catfish female breeding line, Referred to as the hybrid female parent;
1.2、杂交父本的筛选：1.2. Screening of hybrid male parents:
头年4月中旬至11月底，选择体表无伤、体重在300g以上的野生雌性瓦氏黄颡鱼作为初始瓦氏黄颡鱼母本；选择体表无伤、体重在500g以上的野生雄性瓦氏黄颡鱼作为初始瓦氏黄颡鱼父本，初始瓦氏黄颡鱼母本和初始瓦氏黄颡鱼父本选择好后，在人工养殖条件下，经驯食能摄食人工配合饲料后进行初始瓦氏黄颡鱼亲本培育，年后5-7月份，观察初始瓦氏黄颡鱼亲鱼的性腺发育程度，选择发育成熟的初始瓦氏黄颡鱼亲本进行繁殖催产、孵化以及鱼种培育，在瓦氏黄颡鱼苗种快速生长关键阶段，即当年的7-9月份选择生长较快、抗逆能力强的个体，选留1-2％的个体进行培育，第三年达到性成熟，选择雄性作为快速生长杂交黄颡鱼父本选育系，简称杂交父本；From mid-April to the end of November in the first year, select wild female Pelteregryus vacheronii with no injuries on the body surface and a body weight of more than 300g as the initial female parent; select wild males with no injuries on the body surface and a body weight of more than 500g As the initial male parent, the initial female parent and the initial male parent of Peltaceae are selected, and under the conditions of artificial breeding, they can eat artificial compound feed after domestication Afterwards, the parental breeding of the initial P. vacheronii was carried out. From 5 to July after the year, observe the gonad development of the initial P. Cultivation, in the critical stage of the rapid growth of Pelturotus varschii fry, that is, select individuals with faster growth and strong stress resistance in July-September of the year, and select 1-2% of individuals for cultivation, and reach sexual maturity in the third year , select the male as the breeding line of the male parent of the fast-growing hybrid yellow catfish, referred to as the hybrid male parent;
2、杂交亲本的强化培育：2. Intensified cultivation of hybrid parents:
2.1、杂交母本的强化培育：2.1. Intensive cultivation of hybrid female parent:
选择性腺发育良好的杂交母本投放到强化培育池A中，放养密度为260-300斤/亩，待杂交母本适应强化培育池培育环境后，开始驯化摄食专用亲本配合饲料，日投喂量为杂交母本总重量的1-1.5％，每天投喂2次，投喂时间为上午6点至6点半、下午6点半至7点，上午和下午的投喂量分别占日投喂量的30-50％和50-70％，人工催产前1个月，在专用亲本配合饲料中添加质量比重为专用亲本配合饲料质量3％-5％的蝇蛆浆进行2周的杂交母本的强化培育，同时每隔1周对杂交母本进行抽样检查，检查杂交母本性腺的发育情况；The hybrid female parent with well-developed selective glands is put into the intensive cultivation pond A, and the stocking density is 260-300 catties/mu. After the hybrid female parent adapts to the cultivation environment of the intensive cultivation pond, it will start to domesticate and ingest the special parent compound feed. The daily feeding amount It is 1-1.5% of the total weight of the hybrid female parent. It is fed twice a day. The feeding time is from 6:30 am to 6:30 pm and from 6:30 pm to 7 pm. 30-50% and 50-70% of the weight, 1 month before artificial induction, add fly maggot pulp whose mass proportion is 3%-5% of the weight of the special parent compound feed to the special parent compound feed for 2 weeks of hybrid female parent At the same time, the hybrid female parent is sampled every 1 week to check the development of the hybrid female parent's gonad;
2.2、杂交父本的强化培育：2.2. Intensified cultivation of hybrid male parent:
选择性腺发育良好的杂交父本投放到强化培育池B中，放养密度为260-300斤/亩，待杂交父本适应强化培育池培育环境后，开始驯化摄食专用亲本配合饲料，日投喂量为杂交父本总重量的1-1.5％，每天投喂2次，投喂时间为上午6点至6点半、下午6点半至7点，上午和下午的投喂量分别占日投喂量的30-50％和50-70％；The hybrid male parents with well-developed selective glands were put into the intensive cultivation pond B, and the stocking density was 260-300 catties/mu. After the hybrid male parents adapted to the cultivation environment of the intensive cultivation pond, they began to domesticate and ingest the special parent compound feed. The daily feeding amount It is 1-1.5% of the total weight of the hybrid male parent. It is fed twice a day. The feeding time is from 6:00 am to 6:30 pm and from 6:30 pm to 7:00 pm. 30-50% and 50-70% of the volume;
3、人工催产：3. Artificial induced labor:
待杂交母本和杂交父本性腺发育成熟之后，将杂交母本和杂交父本分别投放入两个不同的催产池中分开暂养，杂交母本和杂交父本的投放比例按尾计算为80-100：1，两催产池水温均为25-30℃，杂交母本的放养密度为10-15kg/m2水体，催产期间，催产池处于流水环境，流水流量为0.5-1.0m3/h，待杂交母本第二次注射外源性激素后，加大流水流量，增加流水流量至2.0-3.0m3/h；After the gonads of the hybrid female parent and the hybrid male parent mature, the hybrid female parent and the hybrid male parent are placed in two different oxytocin ponds for separate temporary breeding, and the ratio of the hybrid female parent to the hybrid male parent is calculated as 80% -100:1, the water temperature of the two oxytocin pools is 25-30 ℃, the stocking density of the hybrid female parent is 10-15kg/m 2 water body, and the oxytocin pool is in a flowing water environment during the oxytocin period, and the water flow rate is 0.5-1.0m 3 /h After the second injection of exogenous hormones to the hybrid female parent, increase the water flow rate to 2.0-3.0m 3 /h;
杂交亲本暂养于催产池3-5小时后开始进行人工催产，在杂交母本和杂交父本腹腔胸鳍基部注射外源性激素，杂交母本注射两次外源性激素，两次时间间隔10-12小时，杂交母本第一次注射外源性激素时，外源性激素由鱼用促黄体素释放激素类似物和绒毛膜促性腺激素组成，其各组分用量如下：鱼用促黄体素释放激素类似物10-16ug/kg鱼体、绒毛膜促性腺激素100-200IU/kg鱼体；杂交母本第二次注射外源性激素时，外源性激素由鱼用促黄体素释放激素类似物、绒毛膜促性腺激素和马来酸地欧酮组成，其各组分用量如下：鱼用促黄体素释放激素类似物16-20ug/kg鱼体、绒毛膜促性腺激素1200-2000IU/kg鱼体、马来酸地欧酮4-8mg/kg鱼体；杂交父本注射一次外源性激素，杂交父本注射的外源性激素由鱼用促黄体素释放激素类似物、绒毛膜促性腺激素和马来酸地欧酮组成，其各组分用量如下：鱼用促黄体素释放激素类似物5-8ug/kg鱼体、绒毛膜促性腺激素600-1000IU/kg鱼体、马来酸地欧酮2.0-4.0mg/kg鱼体；The hybrid parent is temporarily raised in the oxytocin pool for 3-5 hours, and artificial oxytocin is started. The exogenous hormone is injected at the base of the abdominal pectoral fin of the hybrid female parent and the hybrid male parent, and the hybrid female parent is injected twice with an interval of 10-12 hours. Hours, when the hybrid female parent is injected with exogenous hormone for the first time, the exogenous hormone is composed of fish luteinizing hormone releasing hormone analogue and chorionic gonadotropin, and the dosage of each component is as follows: fish luteinizing hormone releasing hormone similar 10-16ug/kg fish body, chorionic gonadotropin 100-200IU/kg fish body; when the hybrid female parent is injected with exogenous hormone for the second time, the exogenous hormone is composed of fish luteinizing hormone releasing hormone analogue, chorionic gonadotropin Composed of gonadotropin and dioconone maleate, the dosage of each component is as follows: fish luteinizing hormone releasing hormone analogue 16-20ug/kg fish body, chorionic gonadotropin 1200-2000IU/kg fish body, horse Diolone dioconone 4-8mg/kg fish body; hybrid male parent injected exogenous hormone once, the exogenous hormone injected by hybrid male parent consists of fish luteinizing hormone releasing hormone analogue, chorionic gonadotropin and maleic acid The composition of dioconone, the dosage of each component is as follows: luteinizing hormone releasing hormone analogue for fish 5-8ug/kg fish body, chorionic gonadotropin 600-1000IU/kg fish body, dioconone maleate 2.0- 4.0mg/kg fish body;
4、人工杂交受精4. Artificial cross fertilization
杂交母本第二次注射外源性激素8-12小时后，每隔半小时抽样检测杂交母本性腺排卵状况，待60-70％的个体都能达到顺利完成人工挤卵时，开始人工挤卵收卵，每次收集80-100尾雌鱼鱼卵后，将事先制备好的1尾杂交父本的精液加入到收集的鱼卵中进行人工授精操作；8-12 hours after the second injection of exogenous hormones in the hybrid female parent, sample the ovulation status of the hybrid female parent's gonads every half an hour, and start artificial egg extraction when 60-70% of the individuals can successfully complete the artificial egg extraction To collect eggs, after collecting 80-100 female fish eggs each time, add the semen of one hybrid male parent prepared in advance to the collected fish eggs for artificial insemination;
5、人工孵化：5. Artificial hatching:
将受精卵置于室内孵化箱内进行孵化，孵化密度为60-70万受精卵/m3水体，孵化箱内保持流水环境，使受精卵处于流水环境中，流速为0.2-0.3m/s；Place the fertilized eggs in an indoor incubator for incubation, the hatching density is 600,000-700,000 fertilized eggs/m 3 water body, maintain a flowing water environment in the incubator, so that the fertilized eggs are in a flowing water environment, and the flow rate is 0.2-0.3m/s;
受精卵开始脱膜后4-8小时，用筛网将受精卵分批次捞至不锈钢脸盆中，利用正常发育脱膜受精卵的密度高于死卵和发霉的受精卵的这一特性，剔除掉死卵和发霉的受精卵，将正常发育的受精卵转移至另外已准备好的孵化箱中继续进行孵化；4-8 hours after the fertilized eggs start to demembrane, the fertilized eggs are scooped up in batches into the stainless steel washbasin with a sieve, and the density of the normally developed and demembranized fertilized eggs is higher than that of dead and moldy fertilized eggs. Remove dead eggs and moldy fertilized eggs, and transfer the normally developed fertilized eggs to another prepared incubator for further incubation;
6、苗种的培育：6. Cultivation of seedlings:
当孵化箱内95％以上的受精卵脱膜12小时后，将杂交卵黄苗投放入水泥池中进行暂养培育，放养密度为5-10万尾/m2水体，卵黄苗暂养2-3天后开始出现少量爬墙现象，此时将卵黄苗进行出售或投放入已准备好的专用池塘进行苗种培育，放养密度为20-25万尾/亩，杂交苗种经培育后，即可获得大规格黄优1号黄颡鱼。When more than 95% of the fertilized eggs in the incubator have been removed for 12 hours, put the hybrid yolk seedlings into the cement pond for temporary cultivation. A small amount of wall-climbing phenomenon began to appear after a few days. At this time, the yolk seedlings were sold or put into a prepared special pond for seed cultivation. The stocking density was 200,000-250,000/mu. After the hybrid seedlings were cultivated, they could be obtained Large size Huangyou No. 1 yellow catfish.
进一步，所述的初始黄颡鱼母本和初始黄颡鱼父本是采集自长江流域的湖泊，所述的湖泊为梁子湖、洪湖、洞庭湖或鄱阳湖，所述的野生瓦氏黄颡鱼来自长江流域岳阳段至武汉段。Further, the initial female parent of yellow catfish and the male parent of yellow catfish are collected from lakes in the Yangtze River Basin, the lakes are Liangzi Lake, Hong Lake, Dongting Lake or Poyang Lake, and the wild yellow catfish The fish comes from the section from Yueyang to Wuhan in the Yangtze River Basin.
进一步，所述的催产池水深0.5-0.6m。Further, the water depth of the oxytocin pool is 0.5-0.6m.
进一步，受精卵在孵化箱孵化期间，每隔1-2h清洗过滤筛绢网一次，受精卵开始脱膜后每隔0.5h清洗过滤筛绢网一次。Further, during the hatching period of the fertilized eggs in the incubator, the filter screen silk screen is cleaned once every 1-2h, and the filter screen silk screen is cleaned once every 0.5h after the fertilized eggs start to demembrane.
进一步，所述的水泥池长5-10m、宽1.5-2.0m、高0.8-1.2m，水深0.4-0.6m，所述的专用池塘面积2-4亩，水深0.6-1.0m。Further, the cement pool is 5-10m long, 1.5-2.0m wide, 0.8-1.2m high, and 0.4-0.6m deep, and the dedicated pond has an area of 2-4 mu and a water depth of 0.6-1.0m.
本发明与现有技术相比，其有益效果和优点在于：Compared with the prior art, the present invention has beneficial effects and advantages in that:
1.本发明率先采用群体选育、种间杂交等技术，培育出生长速度明显优于全雄黄颡鱼和普通黄颡鱼，抗逆和抗病改良的黄颡鱼新品种黄优1号，黄优1号的生长速率比普通黄颡鱼提高35.66％以上，比全雄黄颡鱼提高11.94％以上。1. The present invention takes the lead in adopting technologies such as group breeding and interspecific hybridization, and cultivates a new yellow catfish variety Huangyou No. 1 whose growth rate is significantly better than that of all male yellow catfish and common yellow catfish, and has improved stress resistance and disease resistance. The growth rate of Huangyou 1 was increased by more than 35.66% compared with common yellow catfish, and increased by more than 11.94% compared with all male yellow catfish.
2.本发明建立的亲本强化培育方法，具有操作简单、管理容易、效率高、效果明显等特点，保证了亲本的同步性成熟，延长可繁育时间，一年可进行2-3次催产繁育，大大缩短黄颡鱼单次繁育的时间，从而大大提高了黄颡鱼的繁育效率，在黄颡鱼亲本培育等方面，独创性地进行了限食投喂处理和在亲本培育饲料中添加蝇蛆浆对黄颡鱼亲本进行有效的强化培育并取得成功，亲本性成熟在95％以上，杂交母本性腺比重达到13-15％。2. The method for intensified breeding of parents established by the present invention has the characteristics of simple operation, easy management, high efficiency, and obvious effect, which ensures the synchronous maturation of parents, prolongs the breeding time, and can carry out 2-3 times of induced breeding in a year. Greatly shorten the single breeding time of yellow catfish, thus greatly improving the breeding efficiency of yellow catfish. In the cultivation of yellow catfish parents, etc., the original food restriction treatment and the addition of fly maggots to the parent breeding feed The slurry effectively strengthens the breeding of the parents of the yellow catfish and is successful, the sexual maturity of the parents is above 95%, and the proportion of the gonads of the hybrid female reaches 13-15%.
3.本发明建立的黄优1号黄颡鱼生长性状的杂交繁育方法为鱼类高产抗逆品种培育开辟了新的技术途径，对充分开发杂交黄颡鱼黄优1号作为名特优养殖品种创造了最有效的实践措施和手段，具有重大的社会和经济效益。3. The hybrid breeding method for the growth traits of Huangyou No. 1 yellow catfish established by the present invention has opened up a new technical approach for the cultivation of high-yield and stress-resistant varieties of fish, and is useful for fully developing the hybrid yellow catfish No. Huang You No. 1 as a famous, special and excellent culture. Varieties create the most effective practical measures and means, with significant social and economic benefits.
具体实施方式Detailed ways
下面结合具体实施例对本发明进行详细说明。The present invention will be described in detail below in conjunction with specific embodiments.
实施例1Example 1
1、快速生长杂交亲本的筛选：1. Screening of fast-growing hybrid parents:
1.1、母本的筛选：1.1. Screening of female parents:
2010年4月中旬至11月底，收集来自于长江流域周边的湖泊(如梁子湖、洪湖、洞庭湖和鄱阳湖)的野生黄颡鱼，选择体表无伤、体重在100g以上的健壮野生雌性黄颡鱼作为初始黄颡鱼母本；选择体表无伤、体重在250g以上的健壮野生雄性黄颡鱼作为初始黄颡鱼父本，初始黄颡鱼母本和初始黄颡鱼父本选择好后，在人工养殖条件下，经驯食能摄食人工配合饲料后进行初始黄颡鱼亲本培育，2011年5-7月份，观察初始黄颡鱼亲鱼的性腺发育程度，选择发育成熟的黄颡鱼亲本进行繁殖催产、孵化以及鱼种培育，在黄颡鱼苗种快速生长关键阶段(体长5-15cm)，即2011年的7-9月份，选择生长快、抗逆能力强的个体，选留约5％的个体进行培育，直至2012年3-4月份，大部分个体可达到性成熟，选择雌性作为快速生长杂交黄颡鱼母本(A系)，简称杂交母本；From mid-April to the end of November, 2010, wild yellow catfish from lakes around the Yangtze River Basin (such as Liangzi Lake, Hong Lake, Dongting Lake and Poyang Lake) were collected, and healthy wild females weighing more than 100g were selected. The yellow catfish is used as the initial yellow catfish female parent; the robust wild male yellow catfish with no injury on the body surface and a body weight of more than 250g is selected as the initial yellow catfish male parent, and the initial yellow catfish female parent and the initial yellow catfish male parent are selected After harvesting, under the conditions of artificial breeding, after domestication and ingestion of artificial compound feed, the initial parental breeding of yellow catfish was carried out. From May to July 2011, the gonad development degree of the initial parent fish of yellow catfish was observed, and the mature yellow catfish was selected. The fish parents carry out breeding and oxytocin, hatching and fish fingerling cultivation. During the critical stage of rapid growth (body length 5-15cm) of the peltago catfish fry, that is, from July to September in 2011, individuals with fast growth and strong resistance to stress are selected. Keep about 5% of the individuals for cultivation, until March-April 2012, most of the individuals can reach sexual maturity, and select the female as the fast-growing hybrid yellow catfish female parent (A line), referred to as the hybrid female parent;
1.2、父本的筛选：1.2. Screening of male parents:
2009年4月中旬至10月底，收集来自长江流域岳阳段至武汉段的瓦氏黄颡鱼，选择体表无伤、体重在300g以上的健壮野生雌性瓦氏黄颡鱼作为初始瓦氏黄颡鱼母本；选择体表无伤、体重在500g以上的健壮野生雄性瓦氏黄颡鱼作为初始瓦氏黄颡鱼父本，初始瓦氏黄颡鱼母本和初始瓦氏黄颡鱼父本选择好后，在人工养殖条件下，经驯食能摄食人工配合饲料后进行初始瓦氏黄颡鱼亲本培育，2010年5-7月份，观察初始瓦氏黄颡鱼亲鱼的性腺发育程度，选择发育成熟的初始瓦氏黄颡鱼亲本进行繁殖催产、孵化以及鱼种培育，在瓦氏黄颡鱼苗种快速生长关键阶段(体长10-25cm)，即2010年的7-9月份，选择生长较快、抗逆能力强的个体，选留约1％的个体进行培育，至2012年3-4月份左右，大部分个体达到性成熟，选择雄性作为快速生长杂交黄颡鱼父本(B系)，简称杂交父本；From mid-April to the end of October, 2009, we collected Pelvis vacheronii from the Yueyang section to Wuhan section of the Yangtze River Basin, and selected robust wild female Pelvis vacheronii with no injuries on the body surface and a body weight of more than 300g as the initial P. Fish female parent; choose a robust wild male peltaceae with no wounds on the body surface and a body weight of more than 500g as the initial male parent, the initial female parent and the initial male parent After the selection, under the condition of artificial breeding, the initial parents of Peltedelachus vacheronii were cultivated after being able to ingest artificial compound feed through domestication. During May-July 2010, the gonad development degree of the initial parent fishes of Peltschia vacheroni was observed, and selected The mature initial parents of Peltaceae varschii are used for breeding, oxytocin, hatching and fingerling cultivation. During the critical stage of rapid growth of Peltaceae varschii fry (body length 10-25cm), that is, July-September of 2010, select growth About 1% of the individuals who are faster and have strong resistance to stress are selected and cultivated. By about March-April 2012, most of the individuals will reach sexual maturity, and the males will be selected as the male parent of the fast-growing hybrid yellow catfish (Series B ), referred to as the hybrid father;
2、杂交亲本的强化培育：2. Intensified cultivation of hybrid parents:
2.1、杂交母本的强化培育：2.1. Intensive cultivation of hybrid female parent:
2012年的3-4月份，选择性腺发育良好的杂交母本投放到强化培育池A中，放养密度为280斤/亩，待杂交母本适应强化培育池培育环境后，开始驯化摄食专用亲本配合饲料(市售)，日投喂量为杂交母本总重量的1-1.5％，每天投喂2次，投喂时间为上午6点至6点半、下午6点半至7点，上午和下午的投喂量分别占日投喂量的40％和60％，人工催产前1个月，在专用亲本配合饲料中添加质量比重为专用亲本配合饲料质量5％的蝇蛆浆进行2周的杂交母本的强化培育，同时每隔1周对杂交母本进行抽样检查，检查杂交母本性腺的发育情况；From March to April 2012, the hybrid female parent with well-developed selective glands was put into the intensive cultivation pond A, and the stocking density was 280 catties/mu. Feed (commercially available), the daily feeding amount is 1-1.5% of the total weight of the hybrid female parent, and it is fed twice a day. The feeding amount in the afternoon accounted for 40% and 60% of the daily feeding amount respectively. One month before artificial induction, the fly maggot pulp whose mass proportion was 5% of the weight of the special parent compound feed was added to the special parent compound feed for 2 weeks. Intensify the cultivation of the hybrid female parent, and at the same time conduct a sampling inspection of the hybrid female parent every 1 week to check the development of the hybrid female parent's gonads;
2.2、杂交父本的强化培育：2.2. Intensified cultivation of hybrid male parent:
2012年的3-4月份，选择性腺发育良好的杂交父本投放到强化培育池B中，放养密度为280斤/亩，待杂交父本适应强化培育池培育环境后，开始驯化摄食专用亲本配合饲料(市售)，日投喂量为杂交父本总重量的1-1.5％，每天投喂2次，投喂时间为上午6点至6点半、下午6点半至7点，上午和下午的投喂量分别占日投喂量的40％和60％；From March to April in 2012, the hybrid male parents with well-developed selective glands were put into the intensive cultivation pond B, and the stocking density was 280 catties/mu. Feed (commercially available), the daily feeding amount is 1-1.5% of the total weight of the hybrid male parent, and it is fed twice a day, and the feeding time is from 6:30 am to 6:30 pm and from 6:30 pm to 7 pm. The feeding amount in the afternoon accounted for 40% and 60% of the daily feeding amount respectively;
3、人工催产：3. Artificial induced labor:
2012年5月，待杂交母本和杂交父本性腺发育成熟之后，将杂交母本和杂交父本分别投放入两个不同的催产池中分开暂养，杂交母本和杂交父本的投放比例按尾计算为90：1，两催产池水深均为0.5-0.6m，两个催产池水温均为25-30℃，杂交母本的放养密度均为12kg/m2水体，催产期间，催产池处于流水环境，流水流量为0.5m3/h，待杂交母本第二次注射外源性激素后，加大流水流量，增加流水流量至2.0m3/h；In May 2012, after the gonads of the hybrid female parent and the hybrid male parent matured, the hybrid female parent and the hybrid male parent were placed in two different oxytocin pools for separate temporary breeding. The ratio of the hybrid female parent to the hybrid male parent was Calculated according to the tail, it is 90:1. The water depth of the two oxytocin ponds is 0.5-0.6m, the water temperature of the two oxytocin ponds is 25-30°C, and the stocking density of the hybrid female parent is 12kg/m2. In a running water environment, the flow rate of the water is 0.5m 3 /h. After the second injection of exogenous hormones to the hybrid female parent, increase the flow rate of the water to 2.0m 3 /h;
杂交亲本暂养于催产池3-5小时后开始进行人工催产，在杂交母本和杂交父本腹腔胸鳍基部注射外源性激素，杂交母本注射两次外源性激素，两次时间间隔11小时，杂交母本第一次注射外源性激素时，外源性激素由鱼用促黄体素释放激素类似物和绒毛膜促性腺激素组成，其各组分用量如下：鱼用促黄体素释放激素类似物12ug/kg鱼体、绒毛膜促性腺激素175IU/kg鱼体，杂交母本第二次注射外源性激素时，外源性激素由鱼用促黄体素释放激素类似物、绒毛膜促性腺激素和马来酸地欧酮组成，其各组分用量如下：鱼用促黄体素释放激素类似物18ug/kg鱼体、绒毛膜促性腺激素1750IU/kg鱼体、马来酸地欧酮7mg/kg鱼体，杂交父本注射一次外源性激素，杂交父本注射的外源性激素由鱼用促黄体素释放激素类似物、绒毛膜促性腺激素和马来酸地欧酮组成，其各组分用量如下：鱼用促黄体素释放激素类似物6ug/kg鱼体、绒毛膜促性腺激素900IU/kg鱼体、马来酸地欧酮3.0mg/kg鱼体。The hybrid parents are temporarily raised in the oxytocin pool for 3-5 hours, and artificial oxytocin is started. The exogenous hormone is injected at the base of the abdominal pectoral fin of the hybrid female parent and the hybrid male parent, and the hybrid female parent is injected twice with an interval of 11 hours. When the hybrid female parent is injected with exogenous hormone for the first time, the exogenous hormone is composed of fish luteinizing hormone releasing hormone analogue and chorionic gonadotropin, and the dosage of each component is as follows: fish luteinizing hormone releasing hormone analogue 12ug /kg fish body, chorionic gonadotropin 175IU/kg fish body, when the hybrid female parent is injected with exogenous hormone for the second time, the exogenous hormone is composed of fish luteinizing hormone releasing hormone analogue, chorionic gonadotropin and Malay Composition of dioconone acid, the dosage of each component is as follows: luteinizing hormone releasing hormone analogue for fish 18ug/kg fish body, chorionic gonadotropin 1750IU/kg fish body, dioconone maleate 7mg/kg fish body , the hybrid male parent is injected with exogenous hormone once, and the exogenous hormone injected by the hybrid male parent is composed of fish luteinizing hormone releasing hormone analogue, chorionic gonadotropin and dioconone maleate, and the dosage of each component is as follows: Fish use luteinizing hormone releasing hormone analog 6ug/kg fish body, chorionic gonadotropin 900IU/kg fish body, dioconone maleate 3.0mg/kg fish body.
4、人工杂交受精4. Artificial cross fertilization
杂交母本第二次注射外源性激素10小时后，每隔半小时抽样检测杂交母本性腺排卵状况，待60-70％的个体都能达到顺利完成人工挤卵时，即可开始人工挤卵收卵，每次收集90尾雌鱼鱼卵，即可开始将事先制备好的1尾杂交父本的精液加入到鱼卵中进行人工授精操作。10 hours after the second injection of exogenous hormones in the hybrid female parent, sample the ovulation status of the hybrid female parent's gonads every half an hour. When 60-70% of the individuals can successfully complete the artificial egg extraction, the artificial egg extraction can begin To collect eggs, collect 90 female fish eggs each time, and start adding the pre-prepared semen of one hybrid male parent to the eggs for artificial insemination.
5、人工孵化：5. Artificial hatching:
将受精卵置于室内孵化箱内进行孵化，孵化密度为60-70万受精卵/m3水体，孵化箱内保持流水环境，使受精卵处于流水环境中，流速为0.25m/s，调节水流保证受精卵被刚好冲至水表面；Place the fertilized eggs in an indoor incubator for incubation. The hatching density is 600,000 to 700,000 fertilized eggs/m 3 water body. The incubator maintains a flowing water environment so that the fertilized eggs are in a flowing water environment. The flow rate is 0.25m/s, and the water flow is adjusted. Ensure that the fertilized eggs are just flushed to the surface of the water;
受精卵开始脱膜后4-8小时，用80目的筛网将受精卵分批次捞至不锈钢脸盆(直径40cm)中，利用正常发育脱膜的受精卵的密度高于死卵和发霉的受精卵的这一特性，剔除掉死卵和发霉的受精卵，之后将正常发育的受精卵转移至另外已准备好的孵化箱中继续进行孵化，可以防止正常受精卵被霉菌感染，显著性提高受精卵的孵化率。4-8 hours after the fertilized eggs start to demembrane, use an 80-mesh screen to scoop the fertilized eggs into stainless steel washbasins (40cm in diameter) in batches. This characteristic of fertilized eggs removes dead eggs and moldy fertilized eggs, and then transfers the normally developed fertilized eggs to another prepared incubator for continued incubation, which can prevent normal fertilized eggs from being infected by mold and significantly improve hatchability of fertilized eggs.
6、苗种的培育：6. Cultivation of seedlings:
当孵化箱内95％以上的受精卵脱膜12小时后，将卵黄苗投放入水泥池中进行暂时培养，水泥池长10m、宽1.5m、高0.8m，水深0.4-0.6m，放养密度为5-10万尾/m2水体，卵黄苗暂养2-3天后开始出现少量爬墙现象，此时将卵黄苗进行出售或投放入专用池塘进行苗种的培育，专用池塘面积4亩，水深0.6-1.0m，放养密度为20-25万尾/亩，杂交苗种经培育后，获得大规格黄优1号黄颡鱼。When more than 95% of the fertilized eggs in the incubator have been removed for 12 hours, put the yolk seedlings into the cement pool for temporary cultivation. The cement pool is 10m long, 1.5m wide, 0.8m high, and the water depth is 0.4-0.6m. 50,000 to 100,000 tails/m 2 of water body, after 2-3 days of temporary storage, the egg yolk seedlings began to appear a small amount of wall-climbing phenomenon. At this time, the egg yolk seedlings were sold or put into a special pond for seedling cultivation. 0.6-1.0m, the stocking density is 200,000-250,000/mu, and the hybrid seedlings are cultivated to obtain large-scale Huangyou No. 1 yellow catfish.
实验一、本实施例繁育的黄优1号黄颡鱼的生长性能和抗感染能力评测实验Experiment 1. The growth performance and anti-infection ability evaluation experiment of Huangyou No. 1 yellow catfish bred in this example
实验方法：experimental method:
1、当黄优1号黄颡鱼、全雄黄颡鱼和普通黄颡鱼生长至5-8cm时，随机取黄优1号黄颡鱼、全雄黄颡鱼和普通黄颡鱼各400-500尾，挑选规格整齐，体表无外伤，体质健壮的黄优1号黄颡鱼、全雄黄颡鱼和普通黄颡鱼各90尾，测量其平均体长和体重，作为放养时的初始体长和体重，再将挑选好的90尾黄优1号黄颡鱼、全雄黄颡鱼和普通黄颡鱼各随机放养在3个玻璃纤维养殖缸(270L)中，每缸30条，注意调整每缸鱼的重量至各缸之间无显著差异。养殖8周后对黄优1号黄颡鱼、全雄黄颡鱼和普通黄颡鱼的体长和体重进行采样测量，计算黄优1号黄颡鱼、全雄黄颡鱼、普通黄颡鱼的体长和体重的平均值，利用特定增长率和体重增重率来对优1号黄颡鱼、全雄黄颡鱼和普通黄颡鱼的生长性能进行比较评定。1. When Huangyou No. 1 yellow catfish, all male yellow catfish and common yellow catfish grow to 5-8cm, randomly take Huangyou No. 1 yellow catfish, all male yellow catfish and common yellow catfish 400-500 each 90 yellow catfish, all-male yellow catfish and common yellow catfish were selected, and their average body length and weight were measured as the initial body length when stocking. and body weight, then the selected 90 Huangyou No. 1 yellow catfish, all male yellow catfish and common yellow catfish were randomly stocked in 3 fiberglass breeding tanks (270L), 30 in each tank, and attention was paid to adjusting each tank. There was no significant difference in the weight of tank fish between tanks. After 8 weeks of breeding, the body length and weight of Huangyou No. 1 yellow catfish, all male yellow catfish and common yellow catfish were sampled and measured, and the weight of Huangyou No. 1 yellow catfish, all male yellow catfish and common yellow catfish were calculated. The average length and weight of body length and body weight were used to compare and evaluate the growth performance of You 1 yellow catfish, all male yellow catfish and common yellow catfish by using the specific growth rate and body weight gain rate.
2、当黄优1号黄颡鱼、全雄黄颡鱼和普通黄颡鱼生长至5-8cm时，随机取黄优1号黄颡鱼、全雄黄颡鱼和普通黄颡鱼各400-500尾鱼苗，挑选规格整齐，体表无外伤，体质健壮的黄优1号黄颡鱼、全雄黄颡鱼和普通黄颡鱼各180尾，各随机放养在6个玻璃纤维养殖缸(270L)中，每缸30条。将培养好的病原嗜水气单胞菌(Aeromonas hydrophila)用生理盐水稀释成浓度为1×106CFU/ml的菌液，在胸鳍基部注射菌液0.005ml/g鱼体重，攻毒试验各设一对照组(注射相同剂量的生理盐水)，3个平行，实验周期为1周，每天观察记录实验鱼死亡情况。计算各组实验鱼的存活率，利用存活率来对黄优1号黄颡鱼、全雄黄颡鱼和普通黄颡鱼的抗感染能力进行比较评定。2. When Huangyou No. 1 yellow catfish, all male yellow catfish and common yellow catfish grow to 5-8cm, randomly take Huangyou No. 1 yellow catfish, all male yellow catfish and common yellow catfish 400-500 each 180 yellow catfish, all male yellow catfish and common yellow catfish were selected, with neat specifications, no trauma on the body surface, and healthy physique. They were randomly stocked in 6 glass fiber culture tanks (270L). , 30 bars per cylinder. The cultured pathogenic Aeromonas hydrophila (Aeromonas hydrophila) was diluted with physiological saline to a concentration of 1×10 6 CFU/ml bacterial solution, and 0.005ml/g fish body weight of the bacterial solution was injected at the base of the pectoral fin. Set up a control group (injected with the same dose of normal saline), 3 parallels, the experimental period is 1 week, observe and record the death of experimental fish every day. The survival rate of experimental fish in each group was calculated, and the survival rate was used to compare and evaluate the anti-infection ability of Huangyou 1 yellow catfish, all male yellow catfish and common yellow catfish.
实验结果：Experimental results:
优1号黄颡鱼、全雄黄颡鱼、普通黄颡鱼的生长性能和抗感染能力评测结果如下表1所示：The evaluation results of growth performance and anti-infection ability of You No.1 yellow catfish, all male yellow catfish and common yellow catfish are shown in Table 1 below:
表1黄优1号、全雄黄颡鱼和普通黄颡鱼生长性能和抗感染能力测定Table 1 Determination of growth performance and anti-infection ability of Huangyou 1, whole male yellow catfish and common yellow catfish
注：表中数值(平均值±标准差)为3个重复的平均值，数值后不同的上标字母代表差异显著(P<0.05)。Note: The values in the table (mean ± standard deviation) are the average values of 3 repetitions, and different superscript letters after the values represent significant differences (P<0.05).
实施例2Example 2
1、快速生长杂交亲本的筛选：1. Screening of fast-growing hybrid parents:
1.1、母本的筛选：1.1. Screening of female parents:
2012年9月底至11月底，收集来自于长江流域周边的湖泊(如梁子湖、洪湖、洞庭湖和鄱阳湖)的野生黄颡鱼，选择体表无伤、体重在100以上的健壮野生雌性黄颡鱼作为初始黄颡鱼母本；选择体表无伤、体重在250g以上的健壮野生雄性黄颡鱼作为初始黄颡鱼父本，初始黄颡鱼母本和初始黄颡鱼父本选择好后，在人工养殖条件下，经驯食能摄食人工配合饲料后进行初始黄颡鱼亲本培育，2013年5-7月份，观察初始黄颡鱼亲鱼的性腺发育程度，选择发育成熟的黄颡鱼亲本进行繁殖催产、孵化以及鱼种培育，在黄颡鱼苗种快速生长关键阶段(体长5-15cm)，即2013年的7-9月份，选择生长快、抗逆能力强的个体，选留约4％的个体进行培育，直至2014年3-4月份，大部分个体可达到性成熟，选择雌性作为快速生长杂交黄颡鱼母本(A系)，简称杂交母本；From the end of September to the end of November 2012, wild yellow catfish from lakes around the Yangtze River Basin (such as Liangzi Lake, Hong Lake, Dongting Lake and Poyang Lake) were collected, and healthy wild female yellow catfish weighing more than 100 cm were selected. The yellow catfish is used as the initial yellow catfish female parent; the healthy wild male yellow catfish with a body surface of 250g or more is selected as the initial yellow catfish male parent, and the initial yellow catfish female parent and the initial yellow catfish male parent are selected well. Finally, under the condition of artificial breeding, the initial yellow catfish parents were cultivated after being able to eat artificial compound feed after domestication. From May to July 2013, the gonad development degree of the initial yellow catfish broodstock was observed, and mature yellow catfish were selected. Parents carry out breeding and oxytocin, hatching and fish fingerling cultivation. During the critical period of rapid growth of peltago catfish fry (body length 5-15cm), that is, from July to September of 2013, individuals with fast growth and strong resistance to stress are selected and retained. About 4% of the individuals are cultivated until March-April 2014, most of the individuals can reach sexual maturity, and females are selected as the fast-growing hybrid yellow catfish female parent (A line), referred to as the hybrid female parent;
1.2、父本的筛选：1.2. Screening of male parents:
2011年9月底至11月底，收集来自长江流域岳阳段至武汉段的瓦氏黄颡鱼，选择体表无伤、体重在300g以上的健壮野生雌性瓦氏黄颡鱼作为初始瓦氏黄颡鱼母本；选择体表无伤、体重在500g以上的健壮野生雄性瓦氏黄颡鱼作为初始瓦氏黄颡鱼父本，初始瓦氏黄颡鱼母本和初始瓦氏黄颡鱼父本选择好后，在人工养殖条件下，经驯食能摄食人工配合饲料后进行初始瓦氏黄颡鱼亲本培育，2012年5-7月份，观察初始瓦氏黄颡鱼亲鱼的性腺发育程度，选择发育成熟的初始瓦氏黄颡鱼亲本进行繁殖催产、孵化以及鱼种培育，在瓦氏黄颡鱼苗种快速生长关键阶段(体长10-25cm)，即2012的7-9月份，选择生长较快、抗逆能力强的个体，选留约1％的个体进行培育，至2014年3-4月份左右，大部分个体达到性成熟，选择雄性作为快速生长杂交黄颡鱼父本(B系)，简称杂交父本；From the end of September to the end of November, 2011, we collected the Peltaceae vacheronii from the Yueyang section to the Wuhan section of the Yangtze River Basin, and selected robust wild female Pelvetti vacheronii with no injuries on the body surface and a weight of more than 300g as the initial Pelaginella vacheronii The female parent; choose a robust wild male peltaceae with no wounds on the body surface and a body weight of more than 500g as the initial male parent of the pedunculus, and select the initial female and initial males After harvesting, under the condition of artificial breeding, after domestication and ingestion of artificial compound feed, the initial parents of the peltaceae were cultivated. From May to July 2012, the degree of gonad development of the initial broodstock was observed, and the development was selected. The mature initial parents of Peltedelachus vacheronii carry out reproduction and induction, hatching and fish fingerling cultivation. During the critical stage of rapid growth of Peltedelachus vacheronii fry (body length 10-25cm), that is, July-September of 2012, the faster growth is selected , Individuals with strong stress resistance, select and retain about 1% of the individuals for cultivation, and by about March-April 2014, most of the individuals will reach sexual maturity, and males will be selected as the male parent of the fast-growing hybrid yellow catfish (Series B). Referred to as hybrid father;
2、杂交亲本的强化培育：2. Intensified cultivation of hybrid parents:
2.1、杂交母本的强化培育：2.1. Intensive cultivation of hybrid female parent:
2014年的3-4月份，选择性腺发育良好的杂交母本投放到强化培育池A中，放养密度为260斤/亩，待杂交母本适应强化培育池培育环境后，开始驯化摄食专用亲本配合饲料(市售)，日投喂量为杂交母本总重量的1-1.5％，每天投喂2次，投喂时间为上午6点至6点半、下午6点半至7点，上午和下午的投喂量分别占日投喂量的40％和60％，人工催产前1个月，在专用亲本配合饲料中添加质量比重为专用亲本配合饲料质量4％的蝇蛆浆进行2周的杂交母本的强化培育，同时每隔1周对杂交母本进行抽样检查，检查杂交母本性腺的发育情况；From March to April 2014, the hybrid female parent with well-developed selective glands was put into the intensive cultivation pond A, and the stocking density was 260 catties/mu. Feed (commercially available), the daily feeding amount is 1-1.5% of the total weight of the hybrid female parent, and it is fed twice a day. The feeding amount in the afternoon accounted for 40% and 60% of the daily feeding amount respectively. One month before the artificial induction, the fly maggot pulp with a mass proportion of 4% of the weight of the special parent compound feed was added to the special parent compound feed for 2 weeks. Intensify the cultivation of the hybrid female parent, and at the same time conduct a sampling inspection of the hybrid female parent every 1 week to check the development of the hybrid female parent's gonads;
2.2、杂交父本的强化培育：2.2. Intensified cultivation of hybrid male parent:
2014年的3-4月份，选择性腺发育良好的杂交父本投放到强化培育池B中，放养密度为260斤/亩，待杂交父本适应强化培育池培育环境后，开始驯化摄食专用亲本配合饲料(市售)，日投喂量为杂交父本总重量的1-1.5％，每天投喂2次，投喂时间为上午6点至6点半、下午6点半至7点，上午和下午的投喂量分别占日投喂量的40％和60％；From March to April 2014, the hybrid male parents with well-developed selective glands were put into the intensive cultivation pond B, and the stocking density was 260 catties/mu. Feed (commercially available), the daily feeding amount is 1-1.5% of the total weight of the hybrid male parent, and it is fed twice a day, and the feeding time is from 6:30 am to 6:30 pm and from 6:30 pm to 7 pm. The feeding amount in the afternoon accounted for 40% and 60% of the daily feeding amount respectively;
3、人工催产：3. Artificial induced labor:
2014年5月，待杂交母本和杂交父本性腺发育成熟之后，将杂交母本和杂交父本分别投放入两个不同的催产池中分开暂养，杂交母本和杂交父本的投放比例按尾计算为80：1，两个催产池水温均为25-30℃，两个催产池水深为0.5-0.6m，杂交母本的放养密度均为15kg/m2水体，催产期间，催产池处于流水环境，流水流量为0.7m3/h，待杂交母本第二次注射外源性激素后，加大流水流量，增加流水流量至2.5m3/h；In May 2014, after the gonads of the hybrid female parent and the hybrid male parent matured, the hybrid female parent and the hybrid male parent were placed in two different oxytocin pools for separate temporary breeding, and the ratio of the hybrid female parent to the hybrid male parent was Calculated according to the tail, it is 80:1. The water temperature of the two oxytocin ponds is 25-30℃, the water depth of the two oxytocin ponds is 0.5-0.6m, and the stocking density of the hybrid female parent is 15kg/m2. In a running water environment, the flow rate of the water is 0.7m 3 /h. After the second injection of exogenous hormones to the hybrid female parent, increase the flow rate of the water to 2.5m 3 /h;
杂交亲本暂养于催产池3-5小时后开始进行人工催产，在杂交母本和杂交父本腹腔胸鳍基部注射外源性激素，杂交母本注射两次外源性激素，两次时间间隔10小时，杂交母本第一次注射外源性激素时，外源性激素由鱼用促黄体素释放激素类似物和绒毛膜促性腺激素组成，其各组分用量如下：鱼用促黄体素释放激素类似物16ug/kg鱼体、绒毛膜促性腺激素200IU/kg鱼体，杂交母本第二次注射外源性激素时，外源性激素由鱼用促黄体素释放激素类似物、绒毛膜促性腺激素和马来酸地欧酮组成，其各组分用量如下：鱼用促黄体素释放激素类似物16ug/kg鱼体、绒毛膜促性腺激素1200IU/kg鱼体、马来酸地欧酮8mg/kg鱼体，杂交父本注射一次外源性激素，杂交父本注射的外源性激素由鱼用促黄体素释放激素类似物、绒毛膜促性腺激素和马来酸地欧酮组成，其各组分用量如下：鱼用促黄体素释放激素类似物8ug/kg鱼体、绒毛膜促性腺激素1000IU/kg鱼体、马来酸地欧酮2.0mg/kg鱼体。The hybrid parents are temporarily raised in the oxytocin pool for 3-5 hours, and artificial oxytocin is started. The exogenous hormones are injected at the base of the abdominal pectoral fins of the hybrid female parent and the hybrid male parent, and the hybrid female parent is injected twice with an interval of 10 hours. When the hybrid female parent is injected with exogenous hormone for the first time, the exogenous hormone consists of fish luteinizing hormone releasing hormone analogue and chorionic gonadotropin, and the dosage of each component is as follows: fish luteinizing hormone releasing hormone analogue 16ug /kg fish body, chorionic gonadotropin 200IU/kg fish body, when the hybrid female parent is injected with exogenous hormone for the second time, the exogenous hormone is composed of fish luteinizing hormone releasing hormone analogue, chorionic gonadotropin and Malay Composition of dioconone acid, the dosage of each component is as follows: luteinizing hormone releasing hormone analogue for fish 16ug/kg fish body, chorionic gonadotropin 1200IU/kg fish body, dioconone maleate 8mg/kg fish body , the hybrid male parent is injected with exogenous hormone once, and the exogenous hormone injected by the hybrid male parent is composed of fish luteinizing hormone releasing hormone analogue, chorionic gonadotropin and dioconone maleate, and the dosage of each component is as follows: Fish use luteinizing hormone releasing hormone analog 8ug/kg fish body, chorionic gonadotropin 1000IU/kg fish body, dioconone maleate 2.0mg/kg fish body.
4、人工杂交受精4. Artificial cross fertilization
杂交母本第二次注射外源性激素8小时后，每隔半小时抽样检测杂交母本性腺排卵状况，待60-70％的个体都能达到顺利完成人工挤卵时，即可开始人工挤卵收卵，每次收集约80尾雌鱼鱼卵，即可开始将事先制备好的1尾杂交父母的精液加入到鱼卵中进行人工授精操作。8 hours after the second injection of exogenous hormones in the hybrid female parent, sample the ovulation status of the hybrid female parent's gonads every half an hour. When 60-70% of the individuals can successfully complete the artificial egg extraction, the artificial egg extraction can begin To collect eggs, collect about 80 female fish eggs each time, and start adding the pre-prepared semen of one hybrid parent to the eggs for artificial insemination.
5、人工孵化：5. Artificial hatching:
将受精卵置于室内孵化箱内进行孵化，孵化密度为60-70万受精卵/m3水体，孵化箱内保持流水环境，使受精卵处于流水环境中，流速为0.20m/s，调节水流保证受精卵被刚好冲至水表面；Place the fertilized eggs in an indoor incubator for incubation. The hatching density is 600,000 to 700,000 fertilized eggs/m 3 water body. The incubator maintains a flowing water environment so that the fertilized eggs are in a flowing water environment. The flow rate is 0.20m/s, and the water flow is adjusted. Ensure that the fertilized eggs are just flushed to the surface of the water;
受精卵开始脱膜后4-8小时，用80目的筛网将受精卵分批次捞至不锈钢脸盆(直径40cm)中，利用正常发育脱膜的受精卵的密度要高于死卵和发霉的受精卵的这一特性，剔除掉死卵和发霉的受精卵，之后将正常发育的受精卵转移至另外已准备好的孵化箱中继续进行孵化，可以防止正常受精卵被霉菌感染，显著性提高受精卵的孵化率。4-8 hours after the fertilized eggs start to demembrane, use an 80-mesh screen to scoop the fertilized eggs into stainless steel basins (40cm in diameter) in batches. This feature of the fertilized eggs, remove the dead eggs and moldy fertilized eggs, then transfer the fertilized eggs that are normally developed to another prepared incubator for continued incubation, which can prevent the normal fertilized eggs from being infected by mold, significantly Improve the hatching rate of fertilized eggs.
6、苗种的培育：6. Cultivation of seedlings:
当孵化箱内95％以上的受精卵脱膜12小时后，将卵黄苗投放入水泥池中进行暂时培养，水泥池长10m、宽1.5m、高0.8m，水深0.4-0.6m，放养密度为5-10万尾/m2水体，卵黄苗暂养2-3天后开始出现少量爬墙现象，此时将卵黄苗进行出售或投放入专用池塘进行苗种的培育，专用池塘面积3亩，水深0.6-1.0m，放养密度为20-25万尾/亩，杂交苗种经培育后，获得大规格黄优1号黄颡鱼。When more than 95% of the fertilized eggs in the incubator have been removed for 12 hours, put the yolk seedlings into the cement pool for temporary cultivation. The cement pool is 10m long, 1.5m wide, 0.8m high, and the water depth is 0.4-0.6m. 50,000 to 100,000 tails/m 2 of water body, after 2-3 days of temporary storage, a small amount of yolk seedlings will start to climb the wall. At this time, the egg yolk seedlings will be sold or put into a special pond for seedling cultivation. The area of the special pond is 3 mu, and the water depth 0.6-1.0m, the stocking density is 200,000-250,000/mu, and the hybrid seedlings are cultivated to obtain large-scale Huangyou No. 1 yellow catfish.
实验二、本实施例繁育的黄优1号黄颡鱼的生长性能和抗感染能力评测实验Experiment 2. The growth performance and anti-infection ability evaluation experiment of Huangyou No. 1 yellow catfish bred in this embodiment
实验方法：experimental method:
1、当黄优1号黄颡鱼、全雄黄颡鱼和普通黄颡鱼生长至5-8cm时，随机取黄优1号黄颡鱼、全雄黄颡鱼和普通黄颡鱼各400-500尾，挑选规格整齐，体表无外伤，体质健壮的黄优1号黄颡鱼、全雄黄颡鱼和普通黄颡鱼各90尾，测量其平均体长和体重，作为放养时的初始体长和体重，再将挑选好的90尾黄优1号黄颡鱼、全雄黄颡鱼和普通黄颡鱼各随机放养在3个玻璃纤维养殖缸(270L)中，每缸30条，注意调整每缸鱼的重量至各缸之间无显著差异。养殖8周后对黄优1号黄颡鱼、全雄黄颡鱼和普通黄颡鱼的体长和体重进行采样测量，计算黄优1号黄颡鱼、全雄黄颡鱼、普通黄颡鱼的体长和体重的平均值，利用特定增长率和体重增重率来对优1号黄颡鱼、全雄黄颡鱼和普通黄颡鱼的生长性能进行比较评定。1. When Huangyou No. 1 yellow catfish, all male yellow catfish and common yellow catfish grow to 5-8cm, randomly take Huangyou No. 1 yellow catfish, all male yellow catfish and common yellow catfish 400-500 each 90 yellow catfish, all-male yellow catfish and common yellow catfish were selected, and their average body length and weight were measured as the initial body length when stocking. and body weight, then the selected 90 Huangyou No. 1 yellow catfish, all male yellow catfish and common yellow catfish were randomly stocked in 3 fiberglass breeding tanks (270L), 30 in each tank, and attention was paid to adjusting each tank. There was no significant difference in the weight of tank fish between tanks. After 8 weeks of breeding, the body length and weight of Huangyou No. 1 yellow catfish, all male yellow catfish and common yellow catfish were sampled and measured, and the weight of Huangyou No. 1 yellow catfish, all male yellow catfish and common yellow catfish were calculated. The average length and weight of body length and body weight were used to compare and evaluate the growth performance of You 1 yellow catfish, all male yellow catfish and common yellow catfish by using the specific growth rate and body weight gain rate.
2、当黄优1号黄颡鱼、全雄黄颡鱼和普通黄颡鱼生长至5-8cm时，随机取黄优1号黄颡鱼、全雄黄颡鱼和普通黄颡鱼各400-500尾鱼苗，挑选规格整齐，体表无外伤，体质健壮的黄优1号黄颡鱼、全雄黄颡鱼和普通黄颡鱼各180尾，各随机放养在6个玻璃纤维养殖缸(270L)中，每缸30条。将培养好的病原嗜水气单胞菌(Aeromonas hydrophila)用生理盐水稀释成浓度为1×106CFU/ml的菌液，在胸鳍基部注射菌液0.005ml/g鱼体重，攻毒试验各设一对照组(注射相同剂量的生理盐水)，3个平行，实验周期为1周，每天观察记录实验鱼死亡情况。计算各组实验鱼的存活率，利用存活率来对”黄优1号”黄颡鱼、全雄黄颡鱼和普通黄颡鱼的抗感染能力进行比较评定。2. When Huangyou No. 1 yellow catfish, all male yellow catfish and common yellow catfish grow to 5-8cm, randomly take Huangyou No. 1 yellow catfish, all male yellow catfish and common yellow catfish 400-500 each 180 yellow catfish, all male yellow catfish and common yellow catfish were selected, with neat specifications, no trauma on the body surface, and healthy physique. They were randomly stocked in 6 glass fiber culture tanks (270L). , 30 bars per cylinder. The cultured pathogenic Aeromonas hydrophila (Aeromonas hydrophila) was diluted with physiological saline to a concentration of 1×10 6 CFU/ml bacterial solution, and 0.005ml/g fish body weight of the bacterial solution was injected at the base of the pectoral fin. Set up a control group (injected with the same dose of normal saline), 3 parallels, the experimental period is 1 week, observe and record the death of experimental fish every day. The survival rate of experimental fish in each group was calculated, and the survival rate was used to compare and evaluate the anti-infection ability of "Huangyou No. 1" yellow catfish, all male yellow catfish and common yellow catfish.
实验结果：黄优1号黄颡鱼、全雄黄颡鱼、普通黄颡鱼的生长性能和抗感染能力评测结果如下表2所示：Experimental results: The evaluation results of growth performance and anti-infection ability of Huangyou No. 1 yellow catfish, all male yellow catfish, and common yellow catfish are shown in Table 2 below:
表2黄优1号、全雄黄颡鱼和普通黄颡鱼生长性能和抗感染能力测定Table 2 Determination of growth performance and anti-infection ability of Huangyou 1, whole male yellow catfish and common yellow catfish
注：表中数值(平均值±标准差)为3个重复的平均值，数值后不同的上标字母代表差异显著(P<0.05)。Note: The values in the table (mean ± standard deviation) are the average values of 3 repetitions, and different superscript letters after the values represent significant differences (P<0.05).
实施例3Example 3
1、快速生长杂交亲本的筛选：1. Screening of fast-growing hybrid parents:
1.1、母本的筛选：1.1. Screening of female parents:
2013年4月底至11月底，收集来自于长江流域周边的湖泊(如梁子湖、洪湖、洞庭湖和鄱阳湖)的野生黄颡鱼，选择体表无伤、体重在100g以上的健壮野生雌性黄颡鱼作为初始黄颡鱼母本；选择体表无伤、体重在250g以上的健壮野生雄性黄颡鱼作为初始黄颡鱼父本，初始黄颡鱼母本和初始黄颡鱼父本选择好后，在人工养殖条件下，经驯食能摄食人工配合饲料后进行初始黄颡鱼亲本培育，2014年5-7月份，观察初始黄颡鱼亲鱼的性腺发育程度，选择发育成熟的黄颡鱼亲本进行繁殖催产、孵化以及鱼种培育，在黄颡鱼苗种快速生长关键阶段(体长5-15cm)，即2014年的7-9月份，选择生长快、抗逆能力强的个体，选留约5％的个体进行培育，直至2015年3-4月份，大部分个体达到性成熟，选择雌性作为快速生长杂交黄颡鱼母本(A系)，简称杂交母本；From the end of April to the end of November 2013, wild yellow catfish from lakes around the Yangtze River Basin (such as Liangzi Lake, Hong Lake, Dongting Lake and Poyang Lake) were collected, and healthy wild female yellow catfish weighing more than 100g were selected. The yellow catfish is used as the initial yellow catfish female parent; the healthy wild male yellow catfish with a body surface of 250g or more is selected as the initial yellow catfish male parent, and the initial yellow catfish female parent and the initial yellow catfish male parent are selected well. Finally, under the condition of artificial breeding, after domestication and ingestion of artificial compound feed, the initial parental breeding of yellow catfish was carried out. From May to July 2014, the gonad development degree of the initial parent fish of yellow catfish was observed, and mature yellow catfish were selected. The parents carry out breeding and oxytocin, hatching and fish fingerling cultivation. During the critical period of rapid growth of peltago catfish fry (body length 5-15cm), that is, from July to September of 2014, individuals with fast growth and strong resistance to stress are selected and retained. About 5% of the individuals were cultivated until March-April 2015, when most of the individuals reached sexual maturity, and females were selected as the fast-growing hybrid yellow catfish female parent (A line), referred to as the hybrid female parent;
1.2、父本的筛选：1.2. Screening of male parents:
2012年4月底至11月底，收集来自长江流域岳阳段至武汉段的瓦氏黄颡鱼，选择体表无伤、体重在300g以上的健壮野生雌性瓦氏黄颡鱼作为初始瓦氏黄颡鱼母本；选择体表无伤、体重在500g以上的健壮野生雄性瓦氏黄颡鱼作为初始瓦氏黄颡鱼父本，初始瓦氏黄颡鱼母本和初始瓦氏黄颡鱼父本选择好后，在人工养殖条件下，经驯食能摄食人工配合饲料后进行初始瓦氏黄颡鱼亲本培育，2013年5-7月份，观察初始瓦氏黄颡鱼亲鱼的性腺发育程度，选择发育成熟的初始瓦氏黄颡鱼亲本进行繁殖催产、孵化以及鱼种培育，在瓦氏黄颡鱼苗种快速生长关键阶段(体长10-25cm)，即2013的7-9月份，选择生长较快、抗逆能力强的个体，选留约5％的个体进行培育，至2015年3-4月份左右，大部分个体达到性成熟，选择雄性作为快速生长杂交黄颡鱼父本(B系)，简称杂交父本；From the end of April to the end of November, 2012, we collected the Peltaceae vacheronii from the Yueyang section to the Wuhan section of the Yangtze River Basin, and selected robust wild female Pedrocalyx vacheronii with no injuries on the body surface and a body weight of more than 300g as the initial peltrioca vacheronii. The female parent; choose a robust wild male peltaceae with no wounds on the body surface and a body weight of more than 500g as the initial male parent of the pedunculus, and select the initial female and initial males After harvesting, under the conditions of artificial breeding, after domestication and ingestion of artificial compound feed, the initial parents of the peltaceae were cultivated. From May to July 2013, the degree of gonad development of the initial broodstock was observed, and the development was selected. The mature initial parents of Peltedelachus vacheronii carry out reproduction and induction, hatching and fingerling cultivation. During the critical period of rapid growth of Peltedelachus vacheronii fry (body length 10-25cm), that is, July-September of 2013, the faster growth is selected , Individuals with strong stress resistance, select and retain about 5% of the individuals for cultivation, and by about March-April 2015, most of the individuals will reach sexual maturity, and males will be selected as the male parent of the fast-growing hybrid yellow catfish (Series B). Referred to as hybrid father;
2、杂交亲本的强化培育：2. Intensified cultivation of hybrid parents:
2.1、杂交母本的强化培育：2.1. Intensive cultivation of hybrid female parent:
2015年的3-4月份，选择性腺发育良好的杂交母本投放到强化培育池A中，放养密度为300斤/亩，待杂交母本适应强化培育池培育环境后，开始驯化摄食专用亲本配合饲料(市售)，日投喂量为杂交母本总重量的1-1.5％，每天投喂2次，投喂时间为上午6点至6点半、下午6点半至7点，上午和下午的投喂量分别占日投喂量的30％和70％，人工催产前1个月，在专用亲本配合饲料中添加质量比重为专用亲本配合饲料质量3％的蝇蛆浆进行2周的杂交母本的强化培育，同时每隔1周对杂交母本进行抽样检查，检查杂交母本性腺的发育情况；From March to April 2015, the hybrid female parent with well-developed selective glands was put into the intensive cultivation pond A, and the stocking density was 300 catties/mu. Feed (commercially available), the daily feeding amount is 1-1.5% of the total weight of the hybrid female parent, and it is fed twice a day. The feeding amount in the afternoon accounted for 30% and 70% of the daily feeding amount respectively. One month before the artificial induction, the fly maggot pulp with a mass proportion of 3% of the weight of the special parent compound feed was added to the special parent compound feed for 2 weeks. Intensify the cultivation of the hybrid female parent, and at the same time conduct a sampling inspection of the hybrid female parent every 1 week to check the development of the hybrid female parent's gonads;
2.2、杂交父本的强化培育：2.2. Intensified cultivation of hybrid male parent:
2015年的3-4月份，选择性腺发育良好的杂交父本投放到强化培育池B中，放养密度为280斤/亩，待杂交父本适应强化培育池培育环境后，开始驯化摄食专用亲本配合饲料(市售)，日投喂量为杂交父本总重量的1-1.5％，每天投喂2次，投喂时间为上午6点至6点半、下午6点半至7点，上午和下午的投喂量分别占日投喂量的30％和70％；From March to April 2015, the hybrid male parents with well-developed selective glands were put into the intensive cultivation pond B, and the stocking density was 280 catties/mu. Feed (commercially available), the daily feeding amount is 1-1.5% of the total weight of the hybrid male parent, and it is fed twice a day, and the feeding time is from 6:30 am to 6:30 pm and from 6:30 pm to 7 pm. The feeding amount in the afternoon accounted for 30% and 70% of the daily feeding amount respectively;
3、人工催产：3. Artificial induced labor:
2015年5月，待杂交母本和杂交父本性腺发育成熟之后，将杂交母本和杂交父本分别投放入两个不同的催产池中分开暂养，杂交母本和杂交父本的投放比例按尾计算为100：1，两个催产池水深均为0.5-0.6m，两个催产池水温均为25-30℃，杂交母本的放养密度为10kg/m2水体，催产期间，催产池处于流水环境，流水流量为1.0m3/h，待杂交母本第二次注射外源性激素后，加大流水流量，增加流水流量至3.0m3/h；In May 2015, after the gonads of the hybrid female parent and the hybrid male parent matured, the hybrid female parent and the hybrid male parent were placed in two different oxytocin pools for separate temporary breeding, and the ratio of the hybrid female parent to the hybrid male parent was Calculated according to the tail, it is 100:1, the water depth of the two oxytocin ponds is 0.5-0.6m, the water temperature of the two oxytocin ponds is 25-30°C, and the stocking density of the hybrid female parent is 10kg/ m2 of water body. During the oxytocin pond, the oxytocin pond In a running water environment, the flow rate of the water is 1.0m 3 /h. After the second injection of exogenous hormones to the hybrid female parent, increase the flow rate of the water to 3.0m 3 /h;
杂交亲本暂养于催产池3-5小时后开始进行人工催产，在杂交母本和杂交父本腹腔胸鳍基部注射外源性激素，杂交母本注射两次外源性激素，两次时间间隔12小时，杂交母本第一次注射外源性激素时，外源性激素由鱼用促黄体素释放激素类似物和绒毛膜促性腺激素组成，其各组分用量如下：鱼用促黄体素释放激素类似物10ug/kg鱼体、绒毛膜促性腺激素150IU/kg鱼体，杂交母本第二次注射外源性激素时，外源性激素由鱼用促黄体素释放激素类似物、绒毛膜促性腺激素和马来酸地欧酮组成，其各组分用量如下：鱼用促黄体素释放激素类似物20ug/kg鱼体、绒毛膜促性腺激素2000IU/kg鱼体、马来酸地欧酮4mg/kg鱼体，杂交父本注射一次外源性激素，杂交父本注射的外源性激素由鱼用促黄体素释放激素类似物、绒毛膜促性腺激素和马来酸地欧酮组成，其各组分用量如下：鱼用促黄体素释放激素类似物5ug/kg鱼体、绒毛膜促性腺激素600IU/kg鱼体、马来酸地欧酮4mg/kg鱼体。The hybrid parents are temporarily raised in the oxytocin pool for 3-5 hours, and artificial oxytocin is started. The exogenous hormones are injected at the base of the abdominal pectoral fins of the hybrid female parent and the hybrid male parent, and the hybrid female parent is injected twice with an interval of 12 hours. When the hybrid female parent is injected with exogenous hormone for the first time, the exogenous hormone is composed of fish luteinizing hormone releasing hormone analogue and chorionic gonadotropin, and the dosage of each component is as follows: fish luteinizing hormone releasing hormone analogue 10ug /kg fish body, chorionic gonadotropin 150IU/kg fish body, when the hybrid female parent is injected with exogenous hormone for the second time, the exogenous hormone is composed of fish luteinizing hormone releasing hormone analogue, chorionic gonadotropin and Malay Composition of dioconone acid, the dosage of each component is as follows: luteinizing hormone releasing hormone analogue for fish 20ug/kg fish body, chorionic gonadotropin 2000IU/kg fish body, dioconone maleate 4mg/kg fish body , the hybrid male parent is injected with exogenous hormone once, and the exogenous hormone injected by the hybrid male parent is composed of fish luteinizing hormone releasing hormone analogue, chorionic gonadotropin and dioconone maleate, and the dosage of each component is as follows: Fish use luteinizing hormone releasing hormone analog 5ug/kg fish body, chorionic gonadotropin 600IU/kg fish body, dioconone maleate 4mg/kg fish body.
4、人工杂交受精4. Artificial cross fertilization
杂交母本第二次注射外源性激素12小时后，每隔半小时抽样检测杂交母本性腺排卵状况，待60-70％的个体都能达到顺利完成人工挤卵时，即可开始人工挤卵收卵，每次收集约100尾雌鱼鱼卵，即可开始将事先制备好的1尾杂交父母的精液加入到鱼卵中进行人工授精操作。12 hours after the second injection of exogenous hormones in the hybrid female parent, sample the ovulation status of the hybrid female parent's gonads every half hour, and when 60-70% of the individuals can successfully complete the artificial egg extraction, the artificial egg extraction can begin To collect eggs, collect about 100 female fish eggs each time, and start adding the pre-prepared semen of one hybrid parent to the eggs for artificial insemination.
5、人工孵化：5. Artificial hatching:
将受精卵置于室内孵化箱内进行孵化，孵化密度为60-70万受精卵/m3水体，孵化箱内保持流水环境，使受精卵处于流水环境中，流速为0.3m/s，调节水流保证受精卵被刚好冲至水表面；Place the fertilized eggs in an indoor incubator for incubation. The hatching density is 600,000 to 700,000 fertilized eggs/m 3 water body. The incubator maintains a flowing water environment so that the fertilized eggs are in a flowing water environment. The flow rate is 0.3m/s and the water flow is adjusted. Ensure that the fertilized eggs are just flushed to the surface of the water;
受精卵开始脱膜后4-8小时，用80目的筛网将受精卵分批次捞至不锈钢脸盆(直径40cm)中，利用正常发育脱膜的受精卵的密度要高于死卵和发霉的受精卵的这一特性，剔除掉死卵和发霉的受精卵，之后将正常发育的受精卵转移至另外已准备好的孵化箱中继续进行孵化，可以防止正常受精卵被霉菌感染，显著性提高受精卵的孵化率。4-8 hours after the fertilized eggs start to demembrane, use an 80-mesh screen to scoop the fertilized eggs into stainless steel basins (40cm in diameter) in batches. The density of fertilized eggs with normal development is higher than that of dead eggs and mold This feature of the fertilized eggs, remove the dead eggs and moldy fertilized eggs, then transfer the fertilized eggs that are normally developed to another prepared incubator for continued incubation, which can prevent the normal fertilized eggs from being infected by mold, significantly Improve the hatching rate of fertilized eggs.
6、苗种的培育：6. Cultivation of seedlings:
当孵化箱内95％以上的受精卵脱膜12小时后，将卵黄苗投放入水泥池中进行暂时培养，水泥池长10m、宽1.5m、高0.8m，水深0.4-0.6m，放养密度为5-10万尾/m2水体，卵黄苗暂养2-3天后开始出现少量爬墙现象，此时将卵黄苗进行出售或投放入专用池塘进行苗种的培育，专用池塘面积4亩，水深0.6-1.0m，放养密度为20-25万尾/亩，杂交苗种经培育后，获得大规格黄优1号黄颡鱼。When more than 95% of the fertilized eggs in the incubator have been removed for 12 hours, put the yolk seedlings into the cement pool for temporary cultivation. The cement pool is 10m long, 1.5m wide, 0.8m high, and the water depth is 0.4-0.6m. 50,000 to 100,000 tails/m 2 of water body, after 2-3 days of temporary storage, the egg yolk seedlings began to appear a small amount of wall-climbing phenomenon. At this time, the egg yolk seedlings were sold or put into a special pond for seedling cultivation. 0.6-1.0m, the stocking density is 200,000-250,000/mu, and the hybrid seedlings are cultivated to obtain large-scale Huangyou No. 1 yellow catfish.
实验三、本实施例繁育的黄优1号黄颡鱼的生长性能和抗感染能力评测实验Experiment 3. The growth performance and anti-infection ability evaluation experiment of Huangyou No. 1 yellow catfish bred in this example
实验方法：experimental method:
1、当黄优1号黄颡鱼、全雄黄颡鱼和普通黄颡鱼生长至5-8cm时，随机取黄优1号黄颡鱼、全雄黄颡鱼和普通黄颡鱼各400-500尾，挑选规格整齐，体表无外伤，体质健壮的黄优1号黄颡鱼、全雄黄颡鱼和普通黄颡鱼各90尾，测量其平均体长和体重，作为放养时的初始体长和体重，再将挑选好的90尾黄优1号黄颡鱼、全雄黄颡鱼和普通黄颡鱼各随机放养在3个玻璃纤维养殖缸(270L)中，每缸30条，注意调整每缸鱼的重量至各缸之间无显著差异。养殖8周后对黄优1号黄颡鱼、全雄黄颡鱼和普通黄颡鱼的体长和体重进行采样测量，计算黄优1号黄颡鱼、全雄黄颡鱼、普通黄颡鱼的体长和体重的平均值，利用特定增长率和体重增重率来对优1号黄颡鱼、全雄黄颡鱼和普通黄颡鱼的生长性能进行比较评定。1. When Huangyou No. 1 yellow catfish, all male yellow catfish and common yellow catfish grow to 5-8cm, randomly take Huangyou No. 1 yellow catfish, all male yellow catfish and common yellow catfish 400-500 each 90 yellow catfish, all-male yellow catfish and common yellow catfish were selected, and their average body length and weight were measured as the initial body length when stocking. and body weight, then the selected 90 Huangyou No. 1 yellow catfish, all male yellow catfish and common yellow catfish were randomly stocked in 3 fiberglass breeding tanks (270L), 30 in each tank, and attention was paid to adjusting each tank. There was no significant difference in the weight of tank fish between tanks. After 8 weeks of breeding, the body length and weight of Huangyou No. 1 yellow catfish, all male yellow catfish and common yellow catfish were sampled and measured, and the weight of Huangyou No. 1 yellow catfish, all male yellow catfish and common yellow catfish were calculated. The average length and weight of body length and body weight were used to compare and evaluate the growth performance of You 1 yellow catfish, all male yellow catfish and common yellow catfish by using the specific growth rate and body weight gain rate.
2、当黄优1号黄颡鱼、全雄黄颡鱼和普通黄颡鱼生长至5-8cm时，随机取黄优1号黄颡鱼、全雄黄颡鱼和普通黄颡鱼各400-500尾鱼苗，挑选规格整齐，体表无外伤，体质健壮的黄优1号黄颡鱼、全雄黄颡鱼和普通黄颡鱼各180尾，各随机放养在6个玻璃纤维养殖缸(270L)中，每缸30条。将培养好的病原嗜水气单胞菌(Aeromonas hydrophila)用生理盐水稀释成浓度为1×106CFU/ml的菌液，在胸鳍基部注射菌液0.005ml/g鱼体重，攻毒试验各设一对照组(注射相同剂量的生理盐水)，3个平行，实验周期为1周，每天观察记录实验鱼死亡情况。计算各组实验鱼的存活率，利用存活率来对”黄优1号”黄颡鱼、全雄黄颡鱼和普通黄颡鱼的抗感染能力进行比较评定。2. When Huangyou No. 1 yellow catfish, all male yellow catfish and common yellow catfish grow to 5-8cm, randomly take Huangyou No. 1 yellow catfish, all male yellow catfish and common yellow catfish 400-500 each 180 yellow catfish, all male yellow catfish and common yellow catfish were selected, with neat specifications, no trauma on the body surface, and healthy physique. They were randomly stocked in 6 glass fiber culture tanks (270L). , 30 bars per cylinder. The cultured pathogenic Aeromonas hydrophila (Aeromonas hydrophila) was diluted with physiological saline to a concentration of 1×10 6 CFU/ml bacterial solution, and 0.005ml/g fish body weight of the bacterial solution was injected at the base of the pectoral fin. Set up a control group (injected with the same dose of normal saline), 3 parallels, the experimental period is 1 week, observe and record the death of experimental fish every day. The survival rate of experimental fish in each group was calculated, and the survival rate was used to compare and evaluate the anti-infection ability of "Huangyou No. 1" yellow catfish, all male yellow catfish and common yellow catfish.
实验结果：黄优1号黄颡鱼、全雄黄颡鱼、普通黄颡鱼的生长性能和抗感染能力评测结果如下表3所示：Experimental results: The evaluation results of the growth performance and anti-infection ability of Huangyou No. 1 yellow catfish, all male yellow catfish, and common yellow catfish are shown in Table 3 below:
表3黄优1号、全雄黄颡鱼和普通黄颡鱼生长性能和抗感染能力测定Table 3 Determination of growth performance and anti-infection ability of Huangyou 1, whole male yellow catfish and common yellow catfish
注：表中数值(平均值±标准差)为3个重复的平均值，数值后不同的上标字母代表差异显著(P<0.05)。Note: The values in the table (mean ± standard deviation) are the average values of 3 repetitions, and different superscript letters after the values represent significant differences (P<0.05).
综上所述，与全雄黄颡鱼和普通黄颡鱼相比，黄优1号黄颡鱼的生长优势极为明显，抗逆性强。In summary, compared with all male yellow catfish and common yellow catfish, Huangyou No. 1 yellow catfish has obvious growth advantages and strong stress resistance.
Claims (5)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611154851.7A CN106577388B (en) | 2016-12-14 | 2016-12-14 | A hybrid breeding method of Huangyou No. 1 peltrelative catfish with improved growth traits |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611154851.7A CN106577388B (en) | 2016-12-14 | 2016-12-14 | A hybrid breeding method of Huangyou No. 1 peltrelative catfish with improved growth traits |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106577388A CN106577388A (en) | 2017-04-26 |
| CN106577388B true CN106577388B (en) | 2019-08-06 |
Family
ID=58802522
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611154851.7A Active CN106577388B (en) | 2016-12-14 | 2016-12-14 | A hybrid breeding method of Huangyou No. 1 peltrelative catfish with improved growth traits |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106577388B (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107155959B (en) * | 2017-04-28 | 2020-08-11 | 南京师范大学 | A compound breeding method for improving growth traits of yellow catfish |
| CN107278981A (en) * | 2017-07-07 | 2017-10-24 | 佛山市蓝科养殖科技有限公司 | A kind of mating system for hybridizing yellow bone fish |
| CN109479760A (en) * | 2018-10-29 | 2019-03-19 | 南宁学院 | A kind of breeding method of mudskipper fry |
| CN109566488A (en) * | 2018-12-20 | 2019-04-05 | 安徽省农业科学院水产研究所 | A method of hybridization Pelteobagrus fulvidraco artificial propagation |
| CN109673540A (en) * | 2019-01-09 | 2019-04-26 | 湖北太湖港水产产业技术研究院有限公司 | A kind of hybridization Pelteobagrus fulvidraco batch artificial fecundation method |
| CN111387095B (en) * | 2020-04-29 | 2021-09-24 | 黄骅市宏润水产养殖有限公司 | Breeding method for improved variety parent of mullet |
| CN112335582A (en) * | 2020-11-05 | 2021-02-09 | 安徽省农业科学院水产研究所 | Artificial breeding method for hybrid pelteobagrus fulvidraco |
| CN113678760B (en) * | 2021-07-14 | 2023-09-05 | 内江师范学院 | A kind of artificial breeding, selective breeding method for crossbreeding of yellow catfish with disease-resistant traits |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101390503A (en) * | 2008-11-03 | 2009-03-25 | 水利部中国科学院水工程生态研究所 | Method for establishing supermale yellow catfish breeding population by mating supermale fish with physiological female fish |
| CN101707986A (en) * | 2009-11-26 | 2010-05-19 | 江苏省淡水水产研究所 | Pelteobagrus vachelli and pseudobagrus ussuriensis cross breeding method |
| CN104719199A (en) * | 2015-03-15 | 2015-06-24 | 青岛农业大学 | Cross breeding method for pelteobagrus fulvidraco and pseudobagrus vachelli |
| CN105360031A (en) * | 2015-11-23 | 2016-03-02 | 武汉百瑞生物技术有限公司 | Method for quickly establishing cross-bred XY holandric pelteobagrus fulvidraco |
| CN105684962A (en) * | 2015-11-17 | 2016-06-22 | 浙江省淡水水产研究所 | A culter alburnus and triangular bream crossbreed preparation method |
-
2016
- 2016-12-14 CN CN201611154851.7A patent/CN106577388B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101390503A (en) * | 2008-11-03 | 2009-03-25 | 水利部中国科学院水工程生态研究所 | Method for establishing supermale yellow catfish breeding population by mating supermale fish with physiological female fish |
| CN101707986A (en) * | 2009-11-26 | 2010-05-19 | 江苏省淡水水产研究所 | Pelteobagrus vachelli and pseudobagrus ussuriensis cross breeding method |
| CN104719199A (en) * | 2015-03-15 | 2015-06-24 | 青岛农业大学 | Cross breeding method for pelteobagrus fulvidraco and pseudobagrus vachelli |
| CN105684962A (en) * | 2015-11-17 | 2016-06-22 | 浙江省淡水水产研究所 | A culter alburnus and triangular bream crossbreed preparation method |
| CN105360031A (en) * | 2015-11-23 | 2016-03-02 | 武汉百瑞生物技术有限公司 | Method for quickly establishing cross-bred XY holandric pelteobagrus fulvidraco |
Non-Patent Citations (3)
| Title |
|---|
| 黄颡鱼♀与瓦氏黄颡鱼♂的杂交研究;王卫民 等;《淡水渔业》;20020531;第32卷(第3期);第3-5页 |
| 黄颡鱼与瓦氏黄颡鱼杂交技术研究;王明宝 等;《现代农业科技》;20121231(第24期);第273,278页 |
| 黄颡鱼与瓦氏黄颡鱼的规模化杂交繁殖;唐忠林 等;《江苏农业科学》;20161130;第44卷(第10期);第303-304页 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106577388A (en) | 2017-04-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106577388B (en) | A hybrid breeding method of Huangyou No. 1 peltrelative catfish with improved growth traits | |
| CN101066046B (en) | Curled-lip mandarin fish and spot and spotted mandarin fish hybridizing propagation process | |
| CN102047851B (en) | Construction and stock breeding method for cultured grass carp families | |
| CN101940184A (en) | Breeding and culturing method of improved crucian carp | |
| CN102972319A (en) | Method for establishment of hybrid strain between bluntsnout bream and topmouth culter and breeding method of topmouth bream | |
| CN104719199A (en) | Cross breeding method for pelteobagrus fulvidraco and pseudobagrus vachelli | |
| CN103329833A (en) | Cross breeding method for Japanese crucian carp and cyprinus carpio red | |
| CN108377936B (en) | A kind of large-scale production method of whole female mandarin fish | |
| CN108967278A (en) | A kind of artificial fecundation method of Scatophagus argus (Linnaeus) | |
| CN101884311B (en) | Method for constructing tongue sole families and breeding superior families | |
| CN100542402C (en) | A method of inducing natural gynogenetic fish | |
| CN111226839B (en) | Method for breeding hypoxia-resistant hybrid pelteobagrus fulvidraco | |
| CN109329122B (en) | Breeding method of improved Japanese white crucian carp and establishment method of strain thereof | |
| CN115735810A (en) | Method for cultivating full-male opsariichthys bidens with high growth speed | |
| CN101341859B (en) | The Method of Distant Hybridization Between Bolobream and Yellow-tailed Pomfret | |
| CN116210627B (en) | Artificial induced spawning and hatching method for tinca | |
| CN110150192B (en) | Method for breeding siganus oramin parent fish | |
| CN107155971A (en) | Yi Zhong Gan fishes and black carp quasi aromatic hybrid rice breeding method | |
| CN110771538A (en) | Method for constructing large yellow croaker family and breeding excellent family | |
| CN114375912B (en) | Large-scale breeding method for all-male freshwater shrimps | |
| CN202680227U (en) | Loach germ cell incubating device | |
| CN111183929B (en) | Erythroculter ilishaeformis induction pengze crucian gynogenesis method | |
| CN101103713A (en) | A kind of artificial reproduction method of sweetfish | |
| CN114128649A (en) | A selective breeding method for the progeny of distant crosses between the bream and the black-tailed bream | |
| KR101476032B1 (en) | Embryo production method for mackerel |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |