CN106577388B - A kind of cross breeding method of excellent No. 1 Pelteobagrus fulvidraco of Huang of improvement growth traits - Google Patents
A kind of cross breeding method of excellent No. 1 Pelteobagrus fulvidraco of Huang of improvement growth traits Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Marine Sciences & Fisheries (AREA)
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- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a kind of cross breeding methods of excellent No. 1 Pelteobagrus fulvidraco of Huang of improvement growth traits, and its step are as follows: 1, the screening of fast-growth hybrid parent: 1.1, maternal screening；1.2, the screening of male parent；2, the reinforced cultivating of parent；3, artificial induced spawning；4, artificial hybridization is fertilized；5, artificial incubation；6, the cultivation of seed.This method has cultivated Pelteobagrus fulvidraco ♀ and Pelteobagrus vachelli
Description
Technical field
The invention belongs to technical field of aquaculture, and in particular to a kind of excellent No. 1 Pelteobagrus fulvidraco of Huang of improvement growth traits
Cross breeding method is that a kind of generated using fish interspecific hybridization breeding technique, parental line selection and cultivation innovative technology has life
Yellow excellent No. 1 Pelteobagrus fulvidraco of the new varieties of long quick, degeneration-resistant and disease-resistant advantage.
Background technique
Pelteobagrus fulvidraco Pelteobagrus fulvidraco (Richardson) is subordinate to siluriformes (Sliuriformes), Chang
Section (Bagridae), Pelteobagrus (Pelteobagrus), popular name spotted maigre, yellow loud, high-pitched sound, yellow wax etc..Pelteobagrus vachelli
Pelteobagrus vachehi (Richardson) is subordinate to siluriformes (Sliuriformes), Chang section (Bagridae), yellow forehead
Fish category (Pelteobagrus), popular name youth silk, yellow wax, river forehead etc..Pelteobagrus fulvidraco Pelteobagrus fulvidraco and Wa Shi
Pelteobagrus fulvidraco Pelteobagrus vachehi is to belong to fish, is widely distributed in ChangJiang River, is that the more important bottom in China is small
Type economic fish.Pelteobagrus fulvidraco is suitable for cultivating in pond, but its individual is smaller, and the speed of growth is slower, Pelteobagrus vachelli individual
Greatly, growth is rapid, but its hypoxia-resistant capacity and resistance are poor, it is more difficult to be suitable for pond culture.In addition, the most body of Pelteobagrus vachelli
Color is in canescence, is traditionally not easy to receive in the market comsupton of most area.
In recent years, domestic Yellow cartfish adult cultured output was more than 400,000 tons, and more than 80 hundred million yuan of annual value of production.But with yellow forehead
The fast development of fish culture industry, some problems occur in succession, for example, inbred phenomenon is serious, Pelteobagrus fulvidraco germplasm is caused to be degenerated,
The speed of growth reduces, premunition declines, and disease is on the rise.In seed rearing stage parasite, bacterial gill rot disease, Beancurd sheet
The normal cultivation that Pelteobagrus fulvidraco seed has been seriously affected with diseases such as enteritis makes Pelteobagrus fulvidraco seed form rate lower.Every year due to disease
Evil causes the economic loss of Pelteobagrus fulvidraco aquaculture heavy, and the cultivation for having seriously affected Pelteobagrus fulvidraco is promoted.Grow slack-off and disease
Taking place frequently is the bottleneck factor for limiting China Pelteobagrus fulvidraco aquaculture sustainable development, also affect Pelteobagrus fulvidraco coastal provinces and cities into
One step is promoted and cultivation.Therefore, the development of Pelteobagrus fulvidraco aquaculture is strong there is an urgent need to cultivate fast growing, resistance and disease resistance
Improved Varieties.
Hybridization refers between variety classes or carries out between same kind of Different population the side that offspring is cultivated in mating
Method.Since two parents differ farther out on genetic distance, thus the offspring hybridized, i.e. hybridization first filial generation in growth performance and resist
Disease etc. is typically superior to parents, and this phenomenon is known as heterosis, hybrid vigor.Crossbreeding is most important in Crop Genetic Breeding
One of method is cultivated by variety classes hybridization in some cereal crops and some is had on growth performance so far
The Hybrid having a clear superiority, such as hybrid rice and corn etc..It is domestic in recent years in terms of Pelteobagrus fulvidraco genetic breeding research,
Genetically engineered cell line and fish sex control breeding technique (Liu Han duty etc. 2007 are mainly carried out；Xu Hui, 2007；Chen Rongguo etc.,
2013).But in relation to screening and cultivating growth and the strong Pelteobagrus fulvidraco of resistance and Pelteobagrus vachelli carry out hybridization and cultivate growth and anti-
The strong improved Varieties of sick power, have not been reported yet.
Summary of the invention
To solve the above-mentioned problems of the prior art, the present invention provides a kind of excellent No. 1 Huangs of Huang of improvement growth traits
The cross breeding method of forehead fish, this method has cultivated Pelteobagrus fulvidraco ♀ and the first-filial generation F1 of Pelteobagrus vachelli ♂ crossbreed is yellow
Excellent No. 1, body colour is yellowish-brown, and growth vigor is extremely obvious, and resistance is strong.
Realize technical solution used by above-mentioned purpose of the present invention are as follows:
A kind of cross breeding method of excellent No. 1 Pelteobagrus fulvidraco of Huang of improvement growth traits, includes the following steps:
1, the screening of fast-growth hybrid parent:
1.1, the maternal screening of hybridization:
Mid-April in the first year to by the end of November, select body surface without wound, weight 100g or more wild female Pelteobagrus fulvidraco as at the beginning of
Beginning Pelteobagrus fulvidraco is maternal；Select body surface without wound, weight 250g or more wild males Pelteobagrus fulvidraco as initial Pelteobagrus fulvidraco male parent, just
After beginning Pelteobagrus fulvidraco female parent and initial Pelteobagrus fulvidraco male parent choose, under the conditions of artificial breeding, is tamed and dociled and eat the hand fit's feeding that can ingest
Initial Pelteobagrus fulvidraco parent culture is carried out after material, observes the gonad development degree of initial Pelteobagrus fulvidraco parent population in Nian Hou 5-7 month, is selected
The initial Pelteobagrus fulvidraco parent to reach maturity carries out breeding and hastens parturition, hatches and nurture of fish fingerlings, closes in Pelteobagrus fulvidraco seed fast-growth
Key stage, i.e. the 7-9 month of current year, selection grow fast, high-output stress-resistance individual, and the individual for selecting and remain 4-6% is cultivated, the
2 years or reach sexal maturity in third year, female is selected to hybridize Pelteobagrus fulvidraco female parent breeding line, referred to as hybridization female parent as fast-growth；
1.2, the screening of parent sire of hybrid pigs:
Mid-April in the first year, wild female Pelteobagrus vachelli of the selection body surface without wound, weight in 300g or more was made to by the end of November
It is maternal for initial Pelteobagrus vachelli；Select body surface without wound, weight 500g or more wild males Pelteobagrus vachelli as initially
After Pelteobagrus vachelli male parent, initial Pelteobagrus vachelli female parent and initial Pelteobagrus vachelli male parent choose, in artificial breeding condition
Under, initial Pelteobagrus vachelli parent culture is carried out after taming and dociling and eating the artifical compound feed that can ingest, in Nian Hou 5-7 month, observation is initial
The gonad development degree of Pelteobagrus vachelli parent population selects the initial Pelteobagrus vachelli parent to reach maturity to carry out breeding and hastens parturition, incubates
Change and nurture of fish fingerlings, in Pelteobagrus vachelli seed fast-growth critical stage, i.e. select in the 7-9 month of current year growth comparatively fast,
The individual of high-output stress-resistance, the individual for selecting and remain 1-2% are cultivated, and third year reaches sexal maturity, select male as fast fast-growing
Long hybridization Pelteobagrus fulvidraco male parent breeding line, abbreviation parent sire of hybrid pigs；
2, the reinforced cultivating of hybrid parent:
2.1, the maternal reinforced cultivating of hybridization:
Select the well-developed hybridization of sexual gland is maternal to launch into reinforced cultivating pond A, breeding density is 260-300 pounds/acre,
After hybridizing maternal adaptation reinforced cultivating pond nurturing an environment, start to tame dedicated parent's mixed feed of ingesting, day feeding volume is miscellaneous
The 1-1.5% for handing over maternal total weight, feeds 2 times daily, and Feeding time is 6 points of the morning to 6 thirty, 6 pm half to 7 points, on
The feeding volume in noon and afternoon accounts for the 30-50% and 50-70% of day feeding volume respectively, 1 month before artificial induced spawning, matches in dedicated parent
It is maternal to close the fly maggot slurry hybridization in progress 2 weeks that addition quality specific gravity in feed is dedicated parent's mixed feed quality 3%-5%
Reinforced cultivating, while inspection is sampled to hybridization female parent every 1 week, check the developmental state for hybridizing maternal sexual gland；
2.2, the reinforced cultivating of parent sire of hybrid pigs:
The well-developed parent sire of hybrid pigs of sexual gland is selected to launch into reinforced cultivating pond B, breeding density is 260-300 pounds/acre,
After parent sire of hybrid pigs adapts to reinforced cultivating pond nurturing an environment, start to tame dedicated parent's mixed feed of ingesting, day feeding volume is miscellaneous
The 1-1.5% for handing over male parent total weight, feeds 2 times daily, and Feeding time is 6 points of the morning to 6 thirty, 6 pm half to 7 points, on
The feeding volume in noon and afternoon accounts for the 30-50% and 50-70% of day feeding volume respectively；
3, artificial induced spawning:
After hybridizing maternal and parent sire of hybrid pigs gonadal maturation, hybridization female parent and parent sire of hybrid pigs are launched respectively into two
It separates in a different spawning inducing pool and temporarily supports, the dispensing ratio of hybridization female parent and parent sire of hybrid pigs is calculated as 80-100:1 by tail, and two urge
Producing water temperature is 25-30 DEG C, and hybridizing maternal breeding density is 10-15kg/m2Water body, during hastening parturition, spawning inducing pool is in stream
Water environment, flowing water flow are 0.5-1.0m3/ h increases flowing water flow after hybridizing maternal second of injection of exogenous hormone, increases
Add flowing water flow to 2.0-3.0m3/h；
Hybrid parent, which is temporarily supported, to be started to carry out artificial induced spawning after spawning inducing pool 3-5 hours, in hybridization female parent and parent sire of hybrid pigs abdomen
Chamber pectoral fin base portion injection of exogenous hormone hybridizes maternal injection exogenous hormones twice, time interval 10-12 hours twice, miscellaneous
When handing over maternal first time injection of exogenous hormone, exogenous hormones are promoted by fish luteotropin releasing hormone d-ala analog and chorion
Gonadal hormone composition, dosage of each component are as follows: fish luteotropin releasing hormone d-ala analog 10-16ug/kg fish body, chorion
Promoting sexual gland hormone 100-200IU/kg fish body；When hybridizing maternal second of injection of exogenous hormone, exogenous hormones are promoted by fish
Luteinizing hormone releasing hormone analog, human chorionic gonadtropin and maleic acid dioxone composition, dosage of each component are as follows: fish is used
Luteotropin releasing hormone d-ala analog 16-20ug/kg fish body, human chorionic gonadtropin 1200-2000IU/kg fish body, Malaysia
Sour dioxone 4-8mg/kg fish body；Parent sire of hybrid pigs injects an exogenous hormones, and the exogenous hormones of parent sire of hybrid pigs injection are by fish
It is formed with luteotropin releasing hormone d-ala analog, human chorionic gonadtropin and maleic acid dioxone, dosage of each component is as follows:
Fish luteotropin releasing hormone d-ala analog 5-8ug/kg fish body, human chorionic gonadtropin 600-1000IU/kg fish body, Malaysia
Sour dioxone 2.0-4.0mg/kg fish body；
4, artificial hybridization is fertilized
After hybridization is second injection of exogenous hormone 8-12 hours maternal, hybridize maternal sexual gland every half an hour sampling Detection
Ovulation situation starts manually crowded ovum and receives ovum, collect every time when the individual of 60-70% is attained by and smoothly completes manually crowded ovum
After 80-100 tail raun fish-egg, the sperm of the 1 tail parent sire of hybrid pigs prepared in advance is added in the fish-egg of collection and is carried out manually
Insemination operation；
5, artificial incubation:
It will hatch in fertilized eggs incubator disposed within, hatching density is ten thousand fertilized eggs of 60-70/m3Water body, hatching
Flowing water environment is kept in case, is in fertilized eggs in flowing water environment, flow velocity 0.2-0.3m/s；
Fertilized eggs start after demoulding 4-8 hours, are fished out fertilized eggs into stainless steel washbowl in batches with sieve, using normal
The density for developing demoulding fertilized eggs is higher than this characteristic of dead ovum and mouldy fertilized eggs, weeds out dead ovum and mouldy fertilization
The fertilized eggs of normal development are transferred to and continue to hatch in the incubator being in addition ready for by ovum；
6, the cultivation of seed:
After when in incubator 95% or more fertilized eggs demoulding 12 is small, hybridization yolk seedling throwing is put into a cement pool progress
It temporarily supports and cultivates, breeding density is ten thousand tails of 5-10/m2Water body, yolk seedling start to occur climbing wall phenomenon on a small quantity after temporarily supporting 2-3 days, at this time
Yolk seedling is sold or is launched and carries out seed rearing into the dedicated pond that is ready for, breeding density be ten thousand tail of 20-25/
Mu, hybrid seedlings can be obtained yellow excellent No. 1 Pelteobagrus fulvidraco of big specification after cultivating.
Further, the initial Pelteobagrus fulvidraco female parent and initial Pelteobagrus fulvidraco male parent are the lake acquired from the Yangtze river basin, institute
The lake stated is Liangzi Lake, Honghu, Dongting Lake or Poyang Lake, and the wild Pelteobagrus vachelli comes from Yangtze river basin Yueyang section extremely
Wuhan section.
Further, the spawning inducing pool depth of water 0.5-0.6m.
Further, fertilized eggs are during incubator hatching, and primary every 1-2h cleaning filtering bolting silk net, fertilized eggs start to take off
It is primary every 0.5h cleaning filtering bolting silk net after film.
Further, the long 5-10m of the cement pit, width 1.5-2.0m, high 0.8-1.2m, depth of water 0.4-0.6m are described
2-4 mus of pool specialized pool surface product, depth of water 0.6-1.0m.
Compared with prior art, the present invention its advantages and advantage are:
1. the present invention takes the lead in being cultivated the speed of growth using technologies such as unexpected mass incident, interspecific hybridization and being substantially better than all-male Huang
Forehead fish and common Pelteobagrus fulvidraco, the yellow excellent growth rate that No. 1, Huang You 1 of the Pelteobagrus fulvidraco new varieties of degeneration-resistant and disease-resistant improvement is than common
Pelteobagrus fulvidraco improves 35.66% or more, improves 11.94% or more than all-male Pelteobagrus fulvidraco.
2. parent's cultivation method for enhancing that the present invention establishes, with easy to operate, management is easy, high-efficient, effect is obvious
The features such as, it ensure that the synchronization sexal maturity of parent, extension can be bred the time, 2-3 breeding of hastening parturition can be carried out within 1 year, greatly shortened
The time of Pelteobagrus fulvidraco single breeding, to substantially increase the breeding efficiency of Pelteobagrus fulvidraco, in terms of Pelteobagrus fulvidraco, solely
Carried out invasively limit food feed processing and added in parent culture feed fly maggot slurry Pelteobagrus fulvidraco parent is carried out it is effective strong
Change and cultivate and be succeeded, parent's sexal maturity hybridizes maternal sexual gland specific gravity and reach 13-15% 95% or more.
3. the cross breeding method for the excellent No. 1 yellow catfish growing character of Huang that the present invention establishes is the training of fish high-yield anti-adversity
It educates and opens new technological approaches, creating to abundant exploitation hybridization Pelteobagrus fulvidraco yellow excellent No. 1 as famous-particular-excellent breed variety most has
The Practice Measures and means of effect have great social and economic benefit.
Specific embodiment
The present invention is described in detail combined with specific embodiments below.
Embodiment 1
1, the screening of fast-growth hybrid parent:
1.1, maternal screening:
In mid-April, 2010 to by the end of November, collects from lake (such as Liangzi Lake, Honghu, the Dongting Lake on Yangtze river basin periphery
Lake and Poyang Lake) wild Pelteobagrus fulvidraco, select body surface without wound, weight 100g or more healthy and strong wild female Pelteobagrus fulvidraco as at the beginning of
Beginning Pelteobagrus fulvidraco is maternal；Select body surface without wound, weight 250g or more healthy and strong wild males Pelteobagrus fulvidraco as initial Pelteobagrus fulvidraco father
Originally, it after initial Pelteobagrus fulvidraco female parent and initial Pelteobagrus fulvidraco male parent choose, under the conditions of artificial breeding, is tamed and dociled food and can be ingested and manually matched
Initial Pelteobagrus fulvidraco parent culture is carried out after closing feed, observes the gonad development journey of initial Pelteobagrus fulvidraco parent population in 5-7 month in 2011
Degree selects the Pelteobagrus fulvidraco parent to reach maturity to carry out breeding and hastens parturition, hatches and nurture of fish fingerlings, in Pelteobagrus fulvidraco seed fast-growth
Critical stage (the long 5-15cm of body), i.e., 2011 7-9 months, selection grow fast, high-output stress-resistance individual, select and remain about 5%
Individual cultivated, until 3-4 month in 2012, most of individual attainability is mature, select female as fast-growth
It is maternal (A system) to hybridize Pelteobagrus fulvidraco, referred to as hybridizes maternal；
1.2, the screening of male parent:
In mid-April, 2009 to the Pelteobagrus vachelli from Yangtze river basin Yueyang section to Wuhan section by the end of October, is collected, selects
Healthy and strong wild female Pelteobagrus vachelli of the body surface without wound, weight in 300g or more is maternal as initial Pelteobagrus vachelli；Selective body
Table without wound, weight 500g or more healthy and strong wild males Pelteobagrus vachelli as initial Pelteobagrus vachelli male parent, initial Wa Shi
After Pelteobagrus fulvidraco female parent and initial Pelteobagrus vachelli male parent choose, under the conditions of artificial breeding, the hand fit that can ingest is eaten through taming and dociling
Initial Pelteobagrus vachelli parent culture is carried out after feed, observes the sexual gland hair of initial Pelteobagrus vachelli parent population in 5-7 month in 2010
Degree is educated, selects the initial Pelteobagrus vachelli parent to reach maturity to carry out breeding and hastens parturition, hatches and nurture of fish fingerlings, in Wa Shi Huang
Forehead fry kind fast-growth critical stage (the long 10-25cm of body), i.e., 2010 7-9 months, selection grow very fast, anti-adversity ability
Strong individual, the individual for selecting and remain about 1% are cultivated, until or so 3-4 month in 2012, most of individual reaches sexal maturity, choosing
Male is selected as fast-growth hybridization Pelteobagrus fulvidraco male parent (B system), abbreviation parent sire of hybrid pigs；
2, the reinforced cultivating of hybrid parent:
2.1, the maternal reinforced cultivating of hybridization:
It 3-4 month in 2012 selects the well-developed hybridization of sexual gland is maternal to launch into reinforced cultivating pond A, puts in a suitable place to breed close
Degree is 280 pounds/acre, after hybridizing maternal adaptation reinforced cultivating pond nurturing an environment, starts to tame dedicated parent's mixed feed of ingesting
(commercially available), day feeding volume are the 1-1.5% for hybridizing maternal total weight, are fed daily 2 times, and Feeding time is 6 points to 6 points of the morning
Half, the feeding volume in half to 7 points of 6 pm, the morning and afternoon accounts for the 40% and 60% of day feeding volume respectively, 1 before artificial induced spawning
Month, the fly maggot slurry that quality specific gravity is dedicated parent's mixed feed quality 5% is added in dedicated parent's mixed feed to carry out 2 weeks
Hybridize maternal reinforced cultivating, while inspection is sampled to hybridization female parent every 1 week, checks the development feelings for hybridizing maternal sexual gland
Condition；
2.2, the reinforced cultivating of parent sire of hybrid pigs:
It 3-4 month in 2012 selects the well-developed parent sire of hybrid pigs of sexual gland to launch into reinforced cultivating pond B, puts in a suitable place to breed close
Degree is 280 pounds/acre, after parent sire of hybrid pigs adapts to reinforced cultivating pond nurturing an environment, starts to tame dedicated parent's mixed feed of ingesting
(commercially available), day feeding volume are the 1-1.5% of parent sire of hybrid pigs total weight, are fed daily 2 times, and Feeding time is 6 points to 6 points of the morning
Half, the feeding volume in half to 7 points of 6 pm, the morning and afternoon accounts for the 40% and 60% of day feeding volume respectively；
3, artificial induced spawning:
In May, 2012, after hybridizing maternal and parent sire of hybrid pigs gonadal maturation, by hybridization female parent and parent sire of hybrid pigs point
It does not launch to separate in two different spawning inducing pools and temporarily support, the dispensing ratio of hybridization female parent and parent sire of hybrid pigs is calculated as 90 by tail:
1, the two spawning inducing pool depth of waters are 0.5-0.6m, and two spawning inducing pool water temperatures are 25-30 DEG C, hybridize maternal breeding density and are
12kg/m2Water body, during hastening parturition, spawning inducing pool is in flowing water environment, and flowing water flow is 0.5m3/ h is injected wait hybridize maternal second
After exogenous hormones, flowing water flow is increased, increases flowing water flow to 2.0m3/h；
Hybrid parent, which is temporarily supported, to be started to carry out artificial induced spawning after spawning inducing pool 3-5 hours, in hybridization female parent and parent sire of hybrid pigs abdomen
Chamber pectoral fin base portion injection of exogenous hormone hybridizes maternal injection exogenous hormones twice, and time interval 11 hours, hybridization are female twice
When this first time injection of exogenous hormone, exogenous hormones are by fish luteotropin releasing hormone d-ala analog and chorionic gonadotrophin
Hormone composition, dosage of each component are as follows: fish luteotropin releasing hormone d-ala analog 12ug/kg fish body, chorionic gonadotrophin
Hormone 175IU/kg fish body, when hybridizing maternal second of injection of exogenous hormone, exogenous hormones are discharged by fish with luteotropin
Hormone analogs, human chorionic gonadtropin and maleic acid dioxone composition, dosage of each component are as follows: fish is released with luteotropin
Hormone analogs 18ug/kg fish body, human chorionic gonadtropin 1750IU/kg fish body, maleic acid dioxone 7mg/kg fish body are put,
Parent sire of hybrid pigs injects an exogenous hormones, and the exogenous hormones of parent sire of hybrid pigs injection are similar with luteinizing hormone releasing hormone by fish
Object, human chorionic gonadtropin and maleic acid dioxone composition, dosage of each component are as follows: fish luteinizing hormone releasing hormone class
Like object 6ug/kg fish body, human chorionic gonadtropin 900IU/kg fish body, maleic acid dioxone 3.0mg/kg fish body.
4, artificial hybridization is fertilized
After hybridization is second injection of exogenous hormone 10 hours maternal, hybridize maternal sexual gland row every half an hour sampling Detection
Ovate condition can start manually to squeeze ovum receipts ovum, receive every time when the individual of 60-70% is attained by and smoothly completes manually crowded ovum
Collect 90 tail raun fish-eggs, the sperm that can start the 1 tail parent sire of hybrid pigs that will be prepared in advance, which is added in fish-egg, manually to be awarded
Essence operation.
5, artificial incubation:
It will hatch in fertilized eggs incubator disposed within, hatching density is ten thousand fertilized eggs of 60-70/m3Water body, hatching
Flowing water environment is kept in case, is in fertilized eggs in flowing water environment, and flow velocity 0.25m/s adjusts water flow and guarantees that fertilized eggs are rigid
It is good to rush to water surface；
Fertilized eggs start after demoulding 4-8 hours, are fished out fertilized eggs to stainless steel washbowl (diameter in batches with the sieve of 80 mesh
In 40cm), this characteristic of dead ovum and mouldy fertilized eggs is higher than using the density of the fertilized eggs of normal development demoulding, is weeded out
The fertilized eggs of normal development, are transferred in the incubator being in addition ready for continue later by dead ovum and mouldy fertilized eggs
Hatching, can prevent normal fertilization ovum by mycotic infection, and conspicuousness improves the hatching rate of fertilized eggs.
6, the cultivation of seed:
After when in incubator 95% or more fertilized eggs demoulding 12 is small, it is temporary that the throwing of yolk seedling is put into a cement pool progress
Culture, the long 10m of cement pit, width 1.5m, high 0.8m, depth of water 0.4-0.6m, breeding density are ten thousand tails of 5-10/m2Water body, yolk seedling
Start to occur climbing wall phenomenon on a small quantity after temporarily supporting 2-3 days, yolk seedling is sold or launched into dedicated pond progress seed at this time
Cultivation, pool specialized pool surface product 4 mu, depth of water 0.6-1.0m, breeding density be ten thousand tails of 20-25/mu, hybrid seedlings after cultivating,
Obtain yellow excellent No. 1 Pelteobagrus fulvidraco of big specification.
The growth performance of excellent No. 1 Pelteobagrus fulvidraco of Huang of experiment one, the present embodiment breeding and anti-infection ability evaluation and test experiment
Experimental method:
1, when yellow excellent No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco and common yellow catfish growing are to 5-8cm, No. 1 Huang of Huang You is taken at random
Forehead fish, all-male Pelteobagrus fulvidraco and each 400-500 tail of common Pelteobagrus fulvidraco select neat specification, and for body surface without wound, physical strong Huang is excellent
No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco and each 90 tail of common Pelteobagrus fulvidraco measure its average body length and weight, as initial when putting in a suitable place to breed
Body is long and weight, then by yellow excellent No. 1 Pelteobagrus fulvidraco of 90 tails of select, all-male Pelteobagrus fulvidraco and common Pelteobagrus fulvidraco is each puts in a suitable place to breed at random 3
In a glass fibre culturing jar (270L), 30, every cylinder, pay attention to adjusting the weight of every nautilus between each cylinder without significant difference.It supports
And weight long to the body of yellow excellent No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco and common Pelteobagrus fulvidraco carries out sampled measurements after growing 8 weeks, calculates yellow
The average value of the body length and weight of excellent No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco, common Pelteobagrus fulvidraco, utilizes specific growth rate and weight to increase
Rate to the growth performance of excellent No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco and common Pelteobagrus fulvidraco is compared evaluation again.
2, when yellow excellent No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco and common yellow catfish growing are to 5-8cm, No. 1 Huang of Huang You is taken at random
Forehead fish, all-male Pelteobagrus fulvidraco and each 400-500 tail fry of common Pelteobagrus fulvidraco, select neat specification, body surface is physical strong without wound
Yellow excellent No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco and each 180 tail of common Pelteobagrus fulvidraco, it is each to put in a suitable place to breed at random in 6 glass fibre culturing jars
In (270L), 30, every cylinder.Cultured cause of disease Aeromonas hydrophila (Aeromonas hydrophila) is used into physiological saline
Being diluted to concentration is 1 × 106The bacterium solution of CFU/ml injects bacterium solution 0.005ml/g fish body weight in pectoral fin base portion, and challenge test is respectively set
One control group (physiological saline of injection same dose), 3 parallel, and experimental period is 1 week, and it is dead to observe and record experiment fish daily
Situation.The survival rate for calculating each group experiment fish, using survival rate come to yellow excellent No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco and common yellow forehead
The anti-infection ability of fish is compared evaluation.
Experimental result:
Excellent No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco, the growth performance of common Pelteobagrus fulvidraco and anti-infection ability evaluation result such as following table
Shown in 1:
Table 1 is excellent No. 1 yellow, all-male Pelteobagrus fulvidraco and common yellow catfish growing performance and anti-infection ability measurement
Note: numerical value (mean+SD) is 3 duplicate average values in table, and different Superscript letters represent after numerical value
Significant difference (P < 0.05).
Embodiment 2
1, the screening of fast-growth hybrid parent:
1.1, maternal screening:
Lake (such as Liangzi Lake, Honghu, the Dongting Lake from Yangtze river basin periphery are collected to by the end of November in by the end of September, 2012
And Poyang Lake) wild Pelteobagrus fulvidraco, select body surface without wound, weight 100 or more healthy and strong wild female Pelteobagrus fulvidraco as initially
Pelteobagrus fulvidraco is maternal；Select body surface without wound, weight 250g or more healthy and strong wild males Pelteobagrus fulvidraco as initial Pelteobagrus fulvidraco male parent,
After initial Pelteobagrus fulvidraco female parent and initial Pelteobagrus fulvidraco male parent choose, under the conditions of artificial breeding, the hand fit that can ingest is eaten through taming and dociling
Initial Pelteobagrus fulvidraco parent culture is carried out after feed, observes the gonad development degree of initial Pelteobagrus fulvidraco parent population in 5-7 month in 2013,
It selects the Pelteobagrus fulvidraco parent that reaches maturity to carry out breeding to hasten parturition, hatch and nurture of fish fingerlings, be closed in Pelteobagrus fulvidraco seed fast-growth
Key stage (the long 5-15cm of body), i.e., 2013 7-9 months, selection grow fast, high-output stress-resistance individual, select and remain about 4%
Individual is cultivated, until 3-4 month in 2014, most of individual attainability is mature, selects female miscellaneous as fast-growth
It hands over Pelteobagrus fulvidraco maternal (A system), referred to as hybridizes maternal；
1.2, the screening of male parent:
In by the end of September, 2011 to the Pelteobagrus vachelli by the end of November, collected from Yangtze river basin Yueyang section to Wuhan section, selective body
Healthy and strong wild female Pelteobagrus vachelli of the table without wound, weight in 300g or more is maternal as initial Pelteobagrus vachelli；Select body surface
Without wound, weight 500g or more healthy and strong wild males Pelteobagrus vachelli as initial Pelteobagrus vachelli male parent, initial Wa Shi is yellow
After forehead fish female parent and initial Pelteobagrus vachelli male parent choose, under the conditions of artificial breeding, is tamed and dociled and eat the hand fit's feeding that can ingest
Initial Pelteobagrus vachelli parent culture is carried out after material, observes the gonad development of initial Pelteobagrus vachelli parent population in 5-7 month in 2012
Degree selects the initial Pelteobagrus vachelli parent to reach maturity to carry out breeding and hastens parturition, hatches and nurture of fish fingerlings, in Wa Shi Huang forehead
Fry kind fast-growth critical stage (the long 10-25cm of body), i.e., 2012 7-9 month, selection growth are very fast, high-output stress-resistance
Individual, the individual for selecting and remain about 1% are cultivated, until or so 3-4 month in 2014, most of individual reaches sexal maturity, and selection is male
Property as fast-growth hybridize Pelteobagrus fulvidraco male parent (B system), abbreviation parent sire of hybrid pigs；
2, the reinforced cultivating of hybrid parent:
2.1, the maternal reinforced cultivating of hybridization:
It 3-4 month in 2014 selects the well-developed hybridization of sexual gland is maternal to launch into reinforced cultivating pond A, puts in a suitable place to breed close
Degree is 260 pounds/acre, after hybridizing maternal adaptation reinforced cultivating pond nurturing an environment, starts to tame dedicated parent's mixed feed of ingesting
(commercially available), day feeding volume are the 1-1.5% for hybridizing maternal total weight, are fed daily 2 times, and Feeding time is 6 points to 6 points of the morning
Half, the feeding volume in half to 7 points of 6 pm, the morning and afternoon accounts for the 40% and 60% of day feeding volume respectively, 1 before artificial induced spawning
Month, the fly maggot slurry that quality specific gravity is dedicated parent's mixed feed quality 4% is added in dedicated parent's mixed feed to carry out 2 weeks
Hybridize maternal reinforced cultivating, while inspection is sampled to hybridization female parent every 1 week, checks the development feelings for hybridizing maternal sexual gland
Condition；
2.2, the reinforced cultivating of parent sire of hybrid pigs:
It 3-4 month in 2014 selects the well-developed parent sire of hybrid pigs of sexual gland to launch into reinforced cultivating pond B, puts in a suitable place to breed close
Degree is 260 pounds/acre, after parent sire of hybrid pigs adapts to reinforced cultivating pond nurturing an environment, starts to tame dedicated parent's mixed feed of ingesting
(commercially available), day feeding volume are the 1-1.5% of parent sire of hybrid pigs total weight, are fed daily 2 times, and Feeding time is 6 points to 6 points of the morning
Half, the feeding volume in half to 7 points of 6 pm, the morning and afternoon accounts for the 40% and 60% of day feeding volume respectively；
3, artificial induced spawning:
In May, 2014, after hybridizing maternal and parent sire of hybrid pigs gonadal maturation, by hybridization female parent and parent sire of hybrid pigs point
It does not launch to separate in two different spawning inducing pools and temporarily support, the dispensing ratio of hybridization female parent and parent sire of hybrid pigs is calculated as 80 by tail:
1, two spawning inducing pool water temperatures are 25-30 DEG C, and two spawning inducing pool depth of waters are 0.5-0.6m, hybridize maternal breeding density and are
15kg/m2Water body, during hastening parturition, spawning inducing pool is in flowing water environment, and flowing water flow is 0.7m3/ h is injected wait hybridize maternal second
After exogenous hormones, flowing water flow is increased, increases flowing water flow to 2.5m3/h；
Hybrid parent, which is temporarily supported, to be started to carry out artificial induced spawning after spawning inducing pool 3-5 hours, in hybridization female parent and parent sire of hybrid pigs abdomen
Chamber pectoral fin base portion injection of exogenous hormone hybridizes maternal injection exogenous hormones twice, and time interval 10 hours, hybridization are female twice
When this first time injection of exogenous hormone, exogenous hormones are by fish luteotropin releasing hormone d-ala analog and chorionic gonadotrophin
Hormone composition, dosage of each component are as follows: fish luteotropin releasing hormone d-ala analog 16ug/kg fish body, chorionic gonadotrophin
Hormone 200IU/kg fish body, when hybridizing maternal second of injection of exogenous hormone, exogenous hormones are discharged by fish with luteotropin
Hormone analogs, human chorionic gonadtropin and maleic acid dioxone composition, dosage of each component are as follows: fish is released with luteotropin
Hormone analogs 16ug/kg fish body, human chorionic gonadtropin 1200IU/kg fish body, maleic acid dioxone 8mg/kg fish body are put,
Parent sire of hybrid pigs injects an exogenous hormones, and the exogenous hormones of parent sire of hybrid pigs injection are similar with luteinizing hormone releasing hormone by fish
Object, human chorionic gonadtropin and maleic acid dioxone composition, dosage of each component are as follows: fish luteinizing hormone releasing hormone class
Like object 8ug/kg fish body, human chorionic gonadtropin 1000IU/kg fish body, maleic acid dioxone 2.0mg/kg fish body.
4, artificial hybridization is fertilized
After hybridization is second injection of exogenous hormone 8 hours maternal, hybridize maternal sexual gland row every half an hour sampling Detection
Ovate condition can start manually to squeeze ovum receipts ovum, receive every time when the individual of 60-70% is attained by and smoothly completes manually crowded ovum
Intensive 80 tail raun fish-egg, the sperm that can start 1 tail that will be prepared in advance hybridization parent, which is added in fish-egg, to carry out manually
Insemination operation.
5, artificial incubation:
It will hatch in fertilized eggs incubator disposed within, hatching density is ten thousand fertilized eggs of 60-70/m3Water body, hatching
Flowing water environment is kept in case, is in fertilized eggs in flowing water environment, and flow velocity 0.20m/s adjusts water flow and guarantees that fertilized eggs are rigid
It is good to rush to water surface；
Fertilized eggs start after demoulding 4-8 hours, are fished out fertilized eggs to stainless steel washbowl (diameter in batches with the sieve of 80 mesh
In 40cm), this characteristic of dead ovum and mouldy fertilized eggs is higher than using the density of the fertilized eggs of normal development demoulding, is rejected
Fall dead ovum and mouldy fertilized eggs, the fertilized eggs of normal development are transferred in the incubator being in addition ready for later continue into
Row hatching, can prevent normal fertilization ovum by mycotic infection, and conspicuousness improves the hatching rate of fertilized eggs.
6, the cultivation of seed:
After when in incubator 95% or more fertilized eggs demoulding 12 is small, it is temporary that the throwing of yolk seedling is put into a cement pool progress
Culture, the long 10m of cement pit, width 1.5m, high 0.8m, depth of water 0.4-0.6m, breeding density are ten thousand tails of 5-10/m2Water body, yolk seedling
Start to occur climbing wall phenomenon on a small quantity after temporarily supporting 2-3 days, yolk seedling is sold or launched into dedicated pond progress seed at this time
Cultivation, pool specialized pool surface product 3 mu, depth of water 0.6-1.0m, breeding density be ten thousand tails of 20-25/mu, hybrid seedlings after cultivating,
Obtain yellow excellent No. 1 Pelteobagrus fulvidraco of big specification.
The growth performance of excellent No. 1 Pelteobagrus fulvidraco of Huang of experiment two, the present embodiment breeding and anti-infection ability evaluation and test experiment
Experimental method:
1, when yellow excellent No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco and common yellow catfish growing are to 5-8cm, No. 1 Huang of Huang You is taken at random
Forehead fish, all-male Pelteobagrus fulvidraco and each 400-500 tail of common Pelteobagrus fulvidraco select neat specification, and for body surface without wound, physical strong Huang is excellent
No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco and each 90 tail of common Pelteobagrus fulvidraco measure its average body length and weight, as initial when putting in a suitable place to breed
Body is long and weight, then by yellow excellent No. 1 Pelteobagrus fulvidraco of 90 tails of select, all-male Pelteobagrus fulvidraco and common Pelteobagrus fulvidraco is each puts in a suitable place to breed at random 3
In a glass fibre culturing jar (270L), 30, every cylinder, pay attention to adjusting the weight of every nautilus between each cylinder without significant difference.It supports
And weight long to the body of yellow excellent No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco and common Pelteobagrus fulvidraco carries out sampled measurements after growing 8 weeks, calculates yellow
The average value of the body length and weight of excellent No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco, common Pelteobagrus fulvidraco, utilizes specific growth rate and weight to increase
Rate to the growth performance of excellent No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco and common Pelteobagrus fulvidraco is compared evaluation again.
2, when yellow excellent No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco and common yellow catfish growing are to 5-8cm, No. 1 Huang of Huang You is taken at random
Forehead fish, all-male Pelteobagrus fulvidraco and each 400-500 tail fry of common Pelteobagrus fulvidraco, select neat specification, body surface is physical strong without wound
Yellow excellent No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco and each 180 tail of common Pelteobagrus fulvidraco, it is each to put in a suitable place to breed at random in 6 glass fibre culturing jars
In (270L), 30, every cylinder.Cultured cause of disease Aeromonas hydrophila (Aeromonas hydrophila) is used into physiological saline
Being diluted to concentration is 1 × 106The bacterium solution of CFU/ml injects bacterium solution 0.005ml/g fish body weight in pectoral fin base portion, and challenge test is respectively set
One control group (physiological saline of injection same dose), 3 parallel, and experimental period is 1 week, and it is dead to observe and record experiment fish daily
Situation.The survival rate for calculating each group experiment fish, using survival rate come to " Huang You 1 " Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco and common yellow
The anti-infection ability of forehead fish is compared evaluation.
Experimental result: No. 1 Pelteobagrus fulvidraco of Huang You, all-male Pelteobagrus fulvidraco, the growth performance of common Pelteobagrus fulvidraco and anti-infection ability are commented
It is as shown in table 2 below to survey result:
Table 2 is excellent No. 1 yellow, all-male Pelteobagrus fulvidraco and common yellow catfish growing performance and anti-infection ability measurement
Note: numerical value (mean+SD) is 3 duplicate average values in table, and different Superscript letters represent after numerical value
Significant difference (P < 0.05).
Embodiment 3
1, the screening of fast-growth hybrid parent:
1.1, maternal screening:
Lake (such as Liangzi Lake, Honghu, the Dongting Lake from Yangtze river basin periphery are collected to by the end of November in by the end of April, 2013
And Poyang Lake) wild Pelteobagrus fulvidraco, select body surface without wound, weight 100g or more healthy and strong wild female Pelteobagrus fulvidraco as initially
Pelteobagrus fulvidraco is maternal；Select body surface without wound, weight 250g or more healthy and strong wild males Pelteobagrus fulvidraco as initial Pelteobagrus fulvidraco male parent,
After initial Pelteobagrus fulvidraco female parent and initial Pelteobagrus fulvidraco male parent choose, under the conditions of artificial breeding, the hand fit that can ingest is eaten through taming and dociling
Initial Pelteobagrus fulvidraco parent culture is carried out after feed, observes the gonad development degree of initial Pelteobagrus fulvidraco parent population in 5-7 month in 2014,
It selects the Pelteobagrus fulvidraco parent that reaches maturity to carry out breeding to hasten parturition, hatch and nurture of fish fingerlings, be closed in Pelteobagrus fulvidraco seed fast-growth
Key stage (the long 5-15cm of body), i.e., 2014 7-9 months, selection grow fast, high-output stress-resistance individual, select and remain about 5%
Individual is cultivated, until 3-4 month in 2015, most of individual reach sexal maturity, female is selected to hybridize as fast-growth
Pelteobagrus fulvidraco female parent (A system) referred to as hybridizes maternal；
1.2, the screening of male parent:
In by the end of April, 2012 to the Pelteobagrus vachelli by the end of November, collected from Yangtze river basin Yueyang section to Wuhan section, selective body
Healthy and strong wild female Pelteobagrus vachelli of the table without wound, weight in 300g or more is maternal as initial Pelteobagrus vachelli；Select body surface
Without wound, weight 500g or more healthy and strong wild males Pelteobagrus vachelli as initial Pelteobagrus vachelli male parent, initial Wa Shi is yellow
After forehead fish female parent and initial Pelteobagrus vachelli male parent choose, under the conditions of artificial breeding, is tamed and dociled and eat the hand fit's feeding that can ingest
Initial Pelteobagrus vachelli parent culture is carried out after material, observes the gonad development of initial Pelteobagrus vachelli parent population in 5-7 month in 2013
Degree selects the initial Pelteobagrus vachelli parent to reach maturity to carry out breeding and hastens parturition, hatches and nurture of fish fingerlings, in Wa Shi Huang forehead
Fry kind fast-growth critical stage (the long 10-25cm of body), i.e., 2013 7-9 month, selection growth are very fast, high-output stress-resistance
Individual, the individual for selecting and remain about 5% are cultivated, until or so 3-4 month in 2015, most of individual reaches sexal maturity, and selection is male
Property as fast-growth hybridize Pelteobagrus fulvidraco male parent (B system), abbreviation parent sire of hybrid pigs；
2, the reinforced cultivating of hybrid parent:
2.1, the maternal reinforced cultivating of hybridization:
It 3-4 month in 2015 selects the well-developed hybridization of sexual gland is maternal to launch into reinforced cultivating pond A, puts in a suitable place to breed close
Degree is 300 pounds/acre, after hybridizing maternal adaptation reinforced cultivating pond nurturing an environment, starts to tame dedicated parent's mixed feed of ingesting
(commercially available), day feeding volume are the 1-1.5% for hybridizing maternal total weight, are fed daily 2 times, and Feeding time is 6 points to 6 points of the morning
Half, the feeding volume in half to 7 points of 6 pm, the morning and afternoon accounts for the 30% and 70% of day feeding volume respectively, 1 before artificial induced spawning
Month, the fly maggot slurry that quality specific gravity is dedicated parent's mixed feed quality 3% is added in dedicated parent's mixed feed to carry out 2 weeks
Hybridize maternal reinforced cultivating, while inspection is sampled to hybridization female parent every 1 week, checks the development feelings for hybridizing maternal sexual gland
Condition；
2.2, the reinforced cultivating of parent sire of hybrid pigs:
It 3-4 month in 2015 selects the well-developed parent sire of hybrid pigs of sexual gland to launch into reinforced cultivating pond B, puts in a suitable place to breed close
Degree is 280 pounds/acre, after parent sire of hybrid pigs adapts to reinforced cultivating pond nurturing an environment, starts to tame dedicated parent's mixed feed of ingesting
(commercially available), day feeding volume are the 1-1.5% of parent sire of hybrid pigs total weight, are fed daily 2 times, and Feeding time is 6 points to 6 points of the morning
Half, the feeding volume in half to 7 points of 6 pm, the morning and afternoon accounts for the 30% and 70% of day feeding volume respectively；
3, artificial induced spawning:
In May, 2015, after hybridizing maternal and parent sire of hybrid pigs gonadal maturation, by hybridization female parent and parent sire of hybrid pigs point
It does not launch to separate in two different spawning inducing pools and temporarily support, the dispensing ratio of hybridization female parent and parent sire of hybrid pigs is calculated as 100 by tail:
1, two spawning inducing pool depth of waters are 0.5-0.6m, and two spawning inducing pool water temperatures are 25-30 DEG C, hybridize maternal breeding density and are
10kg/m2Water body, during hastening parturition, spawning inducing pool is in flowing water environment, and flowing water flow is 1.0m3/ h is injected wait hybridize maternal second
After exogenous hormones, flowing water flow is increased, increases flowing water flow to 3.0m3/h；
Hybrid parent, which is temporarily supported, to be started to carry out artificial induced spawning after spawning inducing pool 3-5 hours, in hybridization female parent and parent sire of hybrid pigs abdomen
Chamber pectoral fin base portion injection of exogenous hormone hybridizes maternal injection exogenous hormones twice, and time interval 12 hours, hybridization are female twice
When this first time injection of exogenous hormone, exogenous hormones are by fish luteotropin releasing hormone d-ala analog and chorionic gonadotrophin
Hormone composition, dosage of each component are as follows: fish luteotropin releasing hormone d-ala analog 10ug/kg fish body, chorionic gonadotrophin
Hormone 150IU/kg fish body, when hybridizing maternal second of injection of exogenous hormone, exogenous hormones are discharged by fish with luteotropin
Hormone analogs, human chorionic gonadtropin and maleic acid dioxone composition, dosage of each component are as follows: fish is released with luteotropin
Hormone analogs 20ug/kg fish body, human chorionic gonadtropin 2000IU/kg fish body, maleic acid dioxone 4mg/kg fish body are put,
Parent sire of hybrid pigs injects an exogenous hormones, and the exogenous hormones of parent sire of hybrid pigs injection are similar with luteinizing hormone releasing hormone by fish
Object, human chorionic gonadtropin and maleic acid dioxone composition, dosage of each component are as follows: fish luteinizing hormone releasing hormone class
Like object 5ug/kg fish body, human chorionic gonadtropin 600IU/kg fish body, maleic acid dioxone 4mg/kg fish body.
4, artificial hybridization is fertilized
After hybridization is second injection of exogenous hormone 12 hours maternal, hybridize maternal sexual gland row every half an hour sampling Detection
Ovate condition can start manually to squeeze ovum receipts ovum, receive every time when the individual of 60-70% is attained by and smoothly completes manually crowded ovum
Intensive 100 tail raun fish-egg, the sperm that can start 1 tail that will be prepared in advance hybridization parent, which is added in fish-egg, to carry out manually
Insemination operation.
5, artificial incubation:
It will hatch in fertilized eggs incubator disposed within, hatching density is ten thousand fertilized eggs of 60-70/m3Water body, hatching
Flowing water environment is kept in case, is in fertilized eggs in flowing water environment, and flow velocity 0.3m/s adjusts water flow and guarantees fertilized eggs by just
It rushes to water surface；
Fertilized eggs start after demoulding 4-8 hours, are fished out fertilized eggs to stainless steel washbowl (diameter in batches with the sieve of 80 mesh
In 40cm), this characteristic of dead ovum and mouldy fertilized eggs is higher than using the density of the fertilized eggs of normal development demoulding, is rejected
Fall dead ovum and mouldy fertilized eggs, the fertilized eggs of normal development are transferred in the incubator being in addition ready for later continue into
Row hatching, can prevent normal fertilization ovum by mycotic infection, and conspicuousness improves the hatching rate of fertilized eggs.
6, the cultivation of seed:
After when in incubator 95% or more fertilized eggs demoulding 12 is small, it is temporary that the throwing of yolk seedling is put into a cement pool progress
Culture, the long 10m of cement pit, width 1.5m, high 0.8m, depth of water 0.4-0.6m, breeding density are ten thousand tails of 5-10/m2Water body, yolk seedling
Start to occur climbing wall phenomenon on a small quantity after temporarily supporting 2-3 days, yolk seedling is sold or launched into dedicated pond progress seed at this time
Cultivation, pool specialized pool surface product 4 mu, depth of water 0.6-1.0m, breeding density be ten thousand tails of 20-25/mu, hybrid seedlings after cultivating,
Obtain yellow excellent No. 1 Pelteobagrus fulvidraco of big specification.
The growth performance of excellent No. 1 Pelteobagrus fulvidraco of Huang of experiment three, the present embodiment breeding and anti-infection ability evaluation and test experiment
Experimental method:
1, when yellow excellent No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco and common yellow catfish growing are to 5-8cm, No. 1 Huang of Huang You is taken at random
Forehead fish, all-male Pelteobagrus fulvidraco and each 400-500 tail of common Pelteobagrus fulvidraco select neat specification, and for body surface without wound, physical strong Huang is excellent
No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco and each 90 tail of common Pelteobagrus fulvidraco measure its average body length and weight, as initial when putting in a suitable place to breed
Body is long and weight, then by yellow excellent No. 1 Pelteobagrus fulvidraco of 90 tails of select, all-male Pelteobagrus fulvidraco and common Pelteobagrus fulvidraco is each puts in a suitable place to breed at random 3
In a glass fibre culturing jar (270L), 30, every cylinder, pay attention to adjusting the weight of every nautilus between each cylinder without significant difference.It supports
And weight long to the body of yellow excellent No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco and common Pelteobagrus fulvidraco carries out sampled measurements after growing 8 weeks, calculates yellow
The average value of the body length and weight of excellent No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco, common Pelteobagrus fulvidraco, utilizes specific growth rate and weight to increase
Rate to the growth performance of excellent No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco and common Pelteobagrus fulvidraco is compared evaluation again.
2, when yellow excellent No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco and common yellow catfish growing are to 5-8cm, No. 1 Huang of Huang You is taken at random
Forehead fish, all-male Pelteobagrus fulvidraco and each 400-500 tail fry of common Pelteobagrus fulvidraco, select neat specification, body surface is physical strong without wound
Yellow excellent No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco and each 180 tail of common Pelteobagrus fulvidraco, it is each to put in a suitable place to breed at random in 6 glass fibre culturing jars
In (270L), 30, every cylinder.Cultured cause of disease Aeromonas hydrophila (Aeromonas hydrophila) is used into physiological saline
Being diluted to concentration is 1 × 106The bacterium solution of CFU/ml injects bacterium solution 0.005ml/g fish body weight in pectoral fin base portion, and challenge test is respectively set
One control group (physiological saline of injection same dose), 3 parallel, and experimental period is 1 week, and it is dead to observe and record experiment fish daily
Situation.The survival rate for calculating each group experiment fish, using survival rate come to " Huang You 1 " Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco and common yellow
The anti-infection ability of forehead fish is compared evaluation.
Experimental result: No. 1 Pelteobagrus fulvidraco of Huang You, all-male Pelteobagrus fulvidraco, the growth performance of common Pelteobagrus fulvidraco and anti-infection ability are commented
It is as shown in table 3 below to survey result:
Table 3 is excellent No. 1 yellow, all-male Pelteobagrus fulvidraco and common yellow catfish growing performance and anti-infection ability measurement
Note: numerical value (mean+SD) is 3 duplicate average values in table, and different Superscript letters represent after numerical value
Significant difference (P < 0.05).
In conclusion the growth vigor of No. 1 Pelteobagrus fulvidraco of Huang You is extremely bright compared with all-male Pelteobagrus fulvidraco and common Pelteobagrus fulvidraco
Aobvious, resistance is strong.
Claims (5)
1. a kind of cross breeding method of excellent No. 1 Pelteobagrus fulvidraco of Huang of improvement growth traits, includes the following steps:
1.1, the screening of fast-growth hybrid parent:
1.1.1, the maternal screening of hybridization:
Mid-April in the first year to by the end of November, select body surface without wound, weight 100g or more wild female Pelteobagrus fulvidraco as initial Huang
Forehead fish is maternal；Select body surface without wound, weight 250g or more wild males Pelteobagrus fulvidraco as initial Pelteobagrus fulvidraco male parent, it is initial yellow
After forehead fish female parent and initial Pelteobagrus fulvidraco male parent choose, under the conditions of artificial breeding, after taming and dociling and eating the artifical compound feed that can ingest
Initial Pelteobagrus fulvidraco parent culture is carried out, observes the gonad development degree of initial Pelteobagrus fulvidraco parent population, selection development in Nian Hou 5-7 month
Mature initial Pelteobagrus fulvidraco parent carries out breeding and hastens parturition, hatches and nurture of fish fingerlings, in Pelteobagrus fulvidraco seed fast-growth key rank
Section, i.e. the 7-9 month of current year, selection grow fast, high-output stress-resistance individual, and the individual for selecting and remain 4-6% is cultivated, second year
Or third year reaches sexal maturity, selects the female individuals of fast-growth as hybridization Pelteobagrus fulvidraco female parent breeding line, referred to as hybridizes female
This；
1.1.2, the screening of parent sire of hybrid pigs:
Mid-April in the first year to by the end of November, select body surface without wound, weight 300g or more wild female Pelteobagrus vachelli as at the beginning of
Beginning Pelteobagrus vachelli is maternal；Select body surface without wound, weight 500g or more wild males Pelteobagrus vachelli as initial Wa Shi
After Pelteobagrus fulvidraco male parent, initial Pelteobagrus vachelli female parent and initial Pelteobagrus vachelli male parent choose, under the conditions of artificial breeding, warp
Tame and docile food, which can ingest, carries out initial Pelteobagrus vachelli parent culture after artifical compound feed, Nian Hou 5-7 month it is yellow to observe initial Wa Shi
The gonad development degree of forehead fish parent fish, select the initial Pelteobagrus vachelli parent to reach maturity carry out breeding hasten parturition, hatch and
Nurture of fish fingerlings, in Pelteobagrus vachelli seed fast-growth critical stage, i.e. the 7-9 month of current year, selection grows very fast, degeneration-resistant energy
The strong individual of power, the individual for selecting and remain 1-2% are cultivated, and third year reaches sexal maturity, and the male of fast-growth is selected to make
To hybridize Pelteobagrus fulvidraco male parent breeding line, abbreviation parent sire of hybrid pigs；
1.2, the reinforced cultivating of hybrid parent:
1.2.1, the maternal reinforced cultivating of hybridization:
Select the well-developed hybridization of sexual gland is maternal to launch into reinforced cultivating pond A, breeding density is 260-300 pounds/acre, to miscellaneous
After handing over maternal adaptation reinforced cultivating pond nurturing an environment, start to tame dedicated parent's mixed feed of ingesting, day feeding volume is that hybridization is female
The 1-1.5% of this total weight is fed 2 times daily, and Feeding time is half to 7 points of at 6 points in the morning to 6 thirty, 6 pm, the morning, under
The feeding volume at noon accounts for 30-50%, 50-70% of day feeding volume respectively, 1 month before artificial induced spawning, in dedicated parent's mixed feed
The maternal reinforced cultivating of the fly maggot slurry hybridization in progress 2 weeks that middle addition quality specific gravity is dedicated parent's mixed feed quality 3-5%,
Inspection is sampled to hybridization female parent every 1 week simultaneously, checks the developmental state for hybridizing maternal sexual gland；
1.2.2, the reinforced cultivating of parent sire of hybrid pigs:
The well-developed parent sire of hybrid pigs of sexual gland is selected to launch into reinforced cultivating pond B, breeding density is 260-300 pounds/acre, to miscellaneous
After handing over male parent to adapt to reinforced cultivating pond nurturing an environment, start to tame dedicated parent's mixed feed of ingesting, day feeding volume is hybridization father
The 1-1.5% of this total weight is fed 2 times daily, and Feeding time is half to 7 points of at 6 points in the morning to 6 thirty, 6 pm, the morning and
The feeding volume in afternoon accounts for the 30-50% and 50-70% of day feeding volume respectively；
1.3, artificial induced spawning:
After hybridizing maternal and parent sire of hybrid pigs gonadal maturation, hybridization female parent and parent sire of hybrid pigs are launched respectively into two not
It separates in same spawning inducing pool and temporarily supports, the dispensing ratio of hybridization female parent and parent sire of hybrid pigs is calculated as 80-100:1, two spawning inducing pools by tail
Water temperature is 25-30 DEG C, and hybridizing maternal breeding density is 10-15kg/m2Water body, during hastening parturition, spawning inducing pool is in flowing water ring
Border, flowing water flow are 0.5-1.0m3/ h increases flowing water flow after hybridizing maternal second of injection of exogenous hormone, increases stream
Water flow is to 2.0-3.0m3/h；
Hybrid parent, which is temporarily supported, to be started to carry out artificial induced spawning after spawning inducing pool 3-5 hours, in hybridization female parent and parent sire of hybrid pigs abdominal cavity chest
Fin base portion injection of exogenous hormone hybridizes maternal injection exogenous hormones twice, and time interval 10-12 hours twice, hybridization was female
When this first time injection of exogenous hormone, exogenous hormones are by fish luteotropin releasing hormone d-ala analog and chorionic gonadotrophin
Hormone composition, dosage of each component are as follows: fish luteotropin releasing hormone d-ala analog 10-16ug/kg fish body, chorion rush property
Glandular hormone 100-200IU/kg fish body；When hybridizing maternal second of injection of exogenous hormone, exogenous hormones are by fish rush corpus luteum
Hormone-releasing hormone analog, human chorionic gonadtropin and maleic acid dioxone composition, dosage of each component are as follows: fish is yellow with promoting
Voxel releasing hormone analog 16-20ug/kg fish body, human chorionic gonadtropin 1200-2000IU/kg fish body, maleic acid
Europe ketone 4-8mg/kg fish body；Parent sire of hybrid pigs injects an exogenous hormones, and the exogenous hormones of parent sire of hybrid pigs injection are promoted by fish
Luteinizing hormone releasing hormone analog, human chorionic gonadtropin and maleic acid dioxone composition, dosage of each component are as follows: fish is used
Luteotropin releasing hormone d-ala analog 5-8ug/kg fish body, human chorionic gonadtropin 600-1000IU/kg fish body, maleic acid
Europe ketone 2.0-4.0mg/kg fish body；
1.4, artificial hybridization is fertilized
After hybridization is second injection of exogenous hormone 8-12 hours maternal, hybridize maternal sexual gland ovulation every half an hour sampling Detection
Situation starts manually crowded ovum and receives ovum, collect 80- every time when the individual of 60-70% is attained by and smoothly completes manually crowded ovum
After 100 tail raun fish-eggs, the sperm of the 1 tail parent sire of hybrid pigs prepared in advance is added in the fish-egg of collection and carries out artificial insemination
Operation；
1.5, artificial incubation:
It will hatch in fertilized eggs incubator disposed within, hatching density is ten thousand fertilized eggs of 60-70/m3Water body, incubator is interior to be protected
Flowing water environment is held, is in fertilized eggs in flowing water environment, flow velocity 0.2-0.3m/s；
Fertilized eggs start after demoulding 4-8 hours, fish out into stainless steel washbowl fertilized eggs in batches with sieve, utilize normal development
The density of demoulding fertilized eggs is higher than this characteristic of dead ovum and mouldy fertilized eggs, weeds out dead ovum and mouldy fertilized eggs, will
The fertilized eggs of normal development, which are transferred to, to continue to hatch in the incubator being in addition ready for；
1.6, the cultivation of seed:
After when in incubator 95% or more fertilized eggs demoulding 12 is small, hybridization yolk seedling throwing is put into a cement pool and is temporarily supported
It cultivates, breeding density is ten thousand tails of 5-10/m2Water body, yolk seedling starts to occur climbing wall phenomenon on a small quantity after temporarily supporting 2-3 days, at this time by ovum
Huang Miao, which is sold or launched, carries out seed rearing into the dedicated pond being ready for, and breeding density is ten thousand tails of 20-25/mu, miscellaneous
It hands over seed after cultivating, can be obtained yellow excellent No. 1 Pelteobagrus fulvidraco of big specification.
2. the cross breeding method of excellent No. 1 Pelteobagrus fulvidraco of Huang of improvement growth traits according to claim 1, feature exist
In: the initial Pelteobagrus fulvidraco female parent and initial Pelteobagrus fulvidraco male parent be the lake acquired from Yangtze river basin periphery, the lake
For Liangzi Lake, Honghu, Dongting Lake or Poyang Lake, the wild Pelteobagrus vachelli is from Yangtze river basin Yueyang section to Wuhan section.
3. the cross breeding method of excellent No. 1 Pelteobagrus fulvidraco of Huang of improvement growth traits according to claim 1, feature exist
In: the spawning inducing pool depth of water 0.5-0.6m.
4. the cross breeding method of excellent No. 1 Pelteobagrus fulvidraco of Huang of improvement growth traits according to claim 1, feature exist
In: it is primary every 1-2h cleaning filtering bolting silk net during fertilized eggs incubator hatching indoors, after fertilized eggs start demoulding, often
It is primary that filtering bolting silk net is cleaned every 0.5h.
5. the cross breeding method of excellent No. 1 Pelteobagrus fulvidraco of Huang of improvement growth traits according to claim 1, feature exist
In: the long 5-10m of the cement pit, width 1.5-2.0m, high 0.8-1.2m, depth of water 0.4-0.6m, the pool specialized pool surface product
2-4 mus, depth of water 0.6-1.0m.
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| CN107155959B (en) * | 2017-04-28 | 2020-08-11 | 南京师范大学 | Compound breeding method for improving growth traits of pelteobagrus fulvidraco |
| CN107278981A (en) * | 2017-07-07 | 2017-10-24 | 佛山市蓝科养殖科技有限公司 | A kind of mating system for hybridizing yellow bone fish |
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| CN109566488A (en) * | 2018-12-20 | 2019-04-05 | 安徽省农业科学院水产研究所 | A method of hybridization Pelteobagrus fulvidraco artificial propagation |
| CN109673540A (en) * | 2019-01-09 | 2019-04-26 | 湖北太湖港水产产业技术研究院有限公司 | A kind of hybridization Pelteobagrus fulvidraco batch artificial fecundation method |
| CN111387095B (en) * | 2020-04-29 | 2021-09-24 | 黄骅市宏润水产养殖有限公司 | Breeding method for improved variety parent of mullet |
| CN112335582A (en) * | 2020-11-05 | 2021-02-09 | 安徽省农业科学院水产研究所 | Artificial breeding method for hybrid pelteobagrus fulvidraco |
| CN113678760B (en) * | 2021-07-14 | 2023-09-05 | 内江师范学院 | Cross breeding method for artificially breeding and breeding pelteobagrus fulvidraco with disease resistance |
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