CN107155971A - Yi Zhong Gan fishes and black carp quasi aromatic hybrid rice breeding method - Google Patents
Yi Zhong Gan fishes and black carp quasi aromatic hybrid rice breeding method Download PDFInfo
- Publication number
- CN107155971A CN107155971A CN201710477242.3A CN201710477242A CN107155971A CN 107155971 A CN107155971 A CN 107155971A CN 201710477242 A CN201710477242 A CN 201710477242A CN 107155971 A CN107155971 A CN 107155971A
- Authority
- CN
- China
- Prior art keywords
- parent
- gan
- fishes
- black carp
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000251468 Actinopterygii Species 0.000 title claims abstract description 57
- 241001275898 Mylopharyngodon piceus Species 0.000 title claims abstract description 32
- 238000009395 breeding Methods 0.000 title claims abstract description 10
- 240000007594 Oryza sativa Species 0.000 title claims abstract description 9
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 9
- 125000003118 aryl group Chemical group 0.000 title claims abstract description 9
- 235000009566 rice Nutrition 0.000 title claims abstract description 9
- 230000009027 insemination Effects 0.000 claims abstract description 11
- 238000009396 hybridization Methods 0.000 claims abstract description 9
- 230000032696 parturition Effects 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 37
- 230000012447 hatching Effects 0.000 claims description 13
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 10
- 229910052760 oxygen Inorganic materials 0.000 claims description 10
- 239000001301 oxygen Substances 0.000 claims description 10
- 239000000284 extract Substances 0.000 claims description 9
- 102000002322 Egg Proteins Human genes 0.000 claims description 8
- 108010000912 Egg Proteins Proteins 0.000 claims description 8
- 210000004681 ovum Anatomy 0.000 claims description 8
- 239000007921 spray Substances 0.000 claims description 5
- 241000205585 Aquilegia canadensis Species 0.000 claims description 4
- 241000756943 Codonopsis Species 0.000 claims description 4
- 239000009636 Huang Qi Substances 0.000 claims description 4
- 229930012538 Paclitaxel Natural products 0.000 claims description 4
- 240000006079 Schisandra chinensis Species 0.000 claims description 4
- 229920000392 Zymosan Polymers 0.000 claims description 4
- 239000003513 alkali Substances 0.000 claims description 4
- 229940107666 astragalus root Drugs 0.000 claims description 4
- 229940036811 bone meal Drugs 0.000 claims description 4
- 239000002374 bone meal Substances 0.000 claims description 4
- 235000013399 edible fruits Nutrition 0.000 claims description 4
- 230000004927 fusion Effects 0.000 claims description 4
- 230000026109 gonad development Effects 0.000 claims description 4
- 238000005286 illumination Methods 0.000 claims description 4
- 229960001592 paclitaxel Drugs 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 239000002994 raw material Substances 0.000 claims description 4
- 210000000582 semen Anatomy 0.000 claims description 4
- RCINICONZNJXQF-MZXODVADSA-N taxol Chemical compound O([C@@H]1[C@@]2(C[C@@H](C(C)=C(C2(C)C)[C@H](C([C@]2(C)[C@@H](O)C[C@H]3OC[C@]3([C@H]21)OC(C)=O)=O)OC(=O)C)OC(=O)[C@H](O)[C@@H](NC(=O)C=1C=CC=CC=1)C=1C=CC=CC=1)O)C(=O)C1=CC=CC=C1 RCINICONZNJXQF-MZXODVADSA-N 0.000 claims description 4
- 239000011782 vitamin Substances 0.000 claims description 4
- 229940088594 vitamin Drugs 0.000 claims description 4
- 229930003231 vitamin Natural products 0.000 claims description 4
- 235000013343 vitamin Nutrition 0.000 claims description 4
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 4
- 235000008406 SarachaNachtschatten Nutrition 0.000 claims description 3
- 235000004790 Solanum aculeatissimum Nutrition 0.000 claims description 3
- 235000008424 Solanum demissum Nutrition 0.000 claims description 3
- 235000018253 Solanum ferox Nutrition 0.000 claims description 3
- 235000000208 Solanum incanum Nutrition 0.000 claims description 3
- 240000002915 Solanum macrocarpon Species 0.000 claims description 3
- 235000013131 Solanum macrocarpon Nutrition 0.000 claims description 3
- 235000009869 Solanum phureja Nutrition 0.000 claims description 3
- 235000000341 Solanum ptychanthum Nutrition 0.000 claims description 3
- 235000017622 Solanum xanthocarpum Nutrition 0.000 claims description 3
- 208000035199 Tetraploidy Diseases 0.000 claims description 3
- 229910052761 rare earth metal Inorganic materials 0.000 claims description 3
- 150000002910 rare earth metals Chemical class 0.000 claims description 3
- 230000024321 chromosome segregation Effects 0.000 claims description 2
- 238000011534 incubation Methods 0.000 claims 1
- 238000011161 development Methods 0.000 abstract description 2
- 238000002474 experimental method Methods 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 235000013601 eggs Nutrition 0.000 description 13
- 230000037396 body weight Effects 0.000 description 6
- 238000002347 injection Methods 0.000 description 6
- 239000007924 injection Substances 0.000 description 6
- 230000002710 gonadal effect Effects 0.000 description 4
- 230000035800 maturation Effects 0.000 description 4
- 240000002045 Guettarda speciosa Species 0.000 description 3
- 235000001287 Guettarda speciosa Nutrition 0.000 description 3
- 241001482311 Trionychidae Species 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 235000013372 meat Nutrition 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 244000025254 Cannabis sativa Species 0.000 description 2
- 241000252210 Cyprinidae Species 0.000 description 2
- 241000252206 Cypriniformes Species 0.000 description 2
- 241000252233 Cyprinus carpio Species 0.000 description 2
- 108700012941 GNRH1 Proteins 0.000 description 2
- 239000000579 Gonadotropin-Releasing Hormone Substances 0.000 description 2
- 229960004407 chorionic gonadotrophin Drugs 0.000 description 2
- 230000005770 chromosome separation Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000001817 pituitary effect Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 241000209128 Bambusa Species 0.000 description 1
- 101100279441 Caenorhabditis elegans egg-5 gene Proteins 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241000237536 Mytilus edulis Species 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 210000003323 beak Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 239000004478 grain bait Substances 0.000 description 1
- 239000004512 granular bait Substances 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 238000013332 literature search Methods 0.000 description 1
- 235000020638 mussel Nutrition 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- 238000009304 pastoral farming Methods 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000001228 trophic effect Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Polymers & Plastics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physiology (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Birds (AREA)
- Biomedical Technology (AREA)
- Insects & Arthropods (AREA)
- Botany (AREA)
- Mycology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention discloses：Yi Zhong Gan fishes and black carp quasi aromatic hybrid rice breeding method, by the selection of parent, the reinforced cultivating of parent, hasten parturition, artificial insemination, hatch these steps and realize.Li Yong Gan fishes of the present invention hybridize with black carp, the merit of Zong He Gan fishes and black carp, through culture experiment, hybridization F1In generation, shows the features such as natural disposition is gentle, feeding habits are wide, has important application value in production, has promoted the sound development of China's culture fishery.
Description
Technical field
The present invention relates to fish new lines breeding technical field, and in particular to Yi Zhong Gan fishes and black carp quasi aromatic hybrid rice breeding method.
Background technology
Gan fishes, belong to Cypriniformes, and Cyprinidae , Gan category, body is elongated, sub- cylindrical shape, kiss point, in beak.Body carries on the back taupe, belly
Silvery white, dorsal fin, tail fin Dark grey are a kind of large-scale food meat fish, and natural disposition is violent, and fast growth, individual is big, and meat is fresh
U.S., disease resistance is weak, not lower oxygen concentration resistance, belongs to typical predacious fish, it is necessary to be classified simultaneously to Gan fishes during propagating artificially
Separately feed, nor other mild fish nursing of easily arranging in pairs or groups, it is very limited in actually cultivation.In addition the property of , Gan fishes into
Ripe is 3-4 ages, and parent population lays eggs in the 4-6 months in rivers torrent.
Black carp, belongs to Cypriniformes, and Cyprinidae, the head breadth is put down, mouth end position, without.Natural disposition is gentle, and individual is big, and growth is rapid, staple food
Spiral shell, freshwater mussel, shrimp and aquatic insect, 4-5 rheological properties are ripe, belong to the species for being easier to cultivation, and parent population lays eggs in the 5-7 months.
Therefore be necessary that Gan fishes, black carp are studied and tested, to obtain that temper is more gentle, that feeding habits scope is wider is new
Kind.By literature search, do not find and the same or analogous open report of the present invention.
The content of the invention
The deficiency existed for above-mentioned prior art, educates it is an object of the invention to provide Yi Zhong Gan fishes and black carp crosses
The method of kind.
To achieve the above object, the technical solution adopted by the present invention：Yi Zhong Gan fishes and black carp quasi aromatic hybrid rice breeding method,
By the selection of parent, the reinforced cultivating of parent, hasten parturition, artificial insemination, hatch these steps and realize, it is characterised in that
The reinforced cultivating concrete operations of above-mentioned parent are：It will be singled out in Gan fish female parent Fang Ru Gan fish parent cultures pond,
And controlling culture pond water temperature to be 20-24 DEG C, 2-3 months before hybridization start to feed the fruit of Chinese magnoliavine 8- including by weight percentage
15%th, honeysuckle 5-10%, Radix Codonopsis 10-20%, B group vitamin 2-5%, Radix Astragali 5-10%, zymosan 3-5%, Semen Cuscutae powder 5-
10%th, the bait including eel bone meal 10-30%, soft-shelled turtle egg 5-10%, day throwing amount is the tails of 1000-2000kg/ ten thousand, promotes gonad development；
The black carp male parent that will be singled out is put into black carp parent culture pond, and controls water temperature in culture pond to be 16-18 DEG C；
The concrete operations of above-mentioned artificial insemination are：Parent after conscientiously observation is hastened parturition, , Cai Gan fishes when both of which full maturity
Mature egg and the mature sperm of black carp, essence:The volume ratio of ovum is 1:3-5 is sufficiently mixed, i.e., the quantity ratio of smart ovum is more than 5
Hundred million：1, temperature control is at 22-24 DEG C, and time of insemination 3-5min is stand-by；
The concrete operations of above-mentioned hatching are：Take step（4）In embryonated egg, be put into incubator and carry out Lotic hatching, hatching use
Water pH value is controlled in 8.0-8.4, and dissolved oxygen is 6.5-8.0mg/L, and water temperature control is 25-27 DEG C, and illumination is controlled in 7000-
20000Lux, 8.2-8.5 ° of DH of water body hardness, water velocity is 3-5m/min；When hatching was by 2 hours, embryonated egg is put into 0-
1 DEG C, 3-4min in the cold water added with herbal mediciment extract, cold dissolved oxygen in water are maintained at more than 5.0mg/L, suppress its chromosome
Division, makes it smoothly double fusion, forms tetraploid；Then take out and hatched once again in the water body of incubator, until going out
Seedling grows up to spray.
Above-mentioned steps（5）In herbal mediciment be mainly prepared from by the raw material of following parts by weight：20-30 parts of taxol,
10-15 parts of nightshade total alkali, 8-10 parts of angelica extract, 8-10 parts of Astragalus Root P.E, 0.01-0.1 parts of light rare earth.
Compared with prior art, the beneficial effects of the invention are as follows：Li Yong Gan fishes of the present invention and black carp hybridize, Zong He Gan fishes and
The merit of black carp, through culture experiment, hybridization F1In generation, shows the features such as natural disposition is gentle, feeding habits are wide, has in production
There is important application value, promoted the sound development of China's culture fishery.
Embodiment
In conjunction with specific embodiment, next the invention will be further elaborated.
Embodiment one
Gan fishes and black carp quasi aromatic hybrid rice breeding method, comprise the following steps：
（1）The selection of parent：Choose 3 more than age, body weight 7-20kg, gonadal maturation and Jian Kang Gan fishes female be as female
This；The black carp male of 4-5 ages, body weight 500-5000g, gonadal maturation and health is chosen as male parent, 6 before hybridization
Month, just allow female, male in parent independent cultivate；
（2）The reinforced cultivating of parent：By step（1）Pick out in Gan fish female parent Fang Ru Gan fish parent cultures pond, and control
Culture pond water temperature processed is 20 DEG C, start within 2 months before hybridization to feed by the fruit of Chinese magnoliavine 8% by weight percentage, honeysuckle 5%,
Radix Codonopsis 10%, B groups vitamin 2%, the Radix Astragali 5%, zymosan 3%, Semen Cuscutae powder 5%, eel bone meal 10%, soft-shelled turtle egg 5% are formulated
Bait, day throwing amount is the tails of 1000kg/ ten thousand, promotes gonad development；The black carp male parent that will be singled out is put into black carp parent culture
Chi Zhong, and control water temperature in culture pond to be 16 DEG C；
（3）Hasten parturition：To passing through step（2）The injection of this Gan of the Mu of processing fishes is promoted by carp pituitary 0.6mg/kg fish bodies weight, injection
The mixing ocyodinic of Luteinizing hormone releasing hormone A2 60ug/kg fish bodies weight, the IU/kg fish bodies of injection chorionic gonadotrophin 1.5 weight；To male parent
Black carp injects ocyodinic LRH-A 0.25ug+HCG100 IU/kg fish body weights,；
（4）Artificial insemination：Conscientious observation of steps（3）Parent, the mature egg of , Cai Gan fishes and green grass or young crops when both of which full maturity
The mature sperm of fish, essence:The volume ratio of ovum is 1:3 are sufficiently mixed, i.e., the quantity ratio of smart ovum is more than 500,000,000：1, temperature control
At 22 DEG C, time of insemination 3min is stand-by；
（5）Hatching：Take step（4）In embryonated egg, be put into incubator and carry out Lotic hatching, Spawning water pH value control exist
8.0, dissolved oxygen is 6.5mg/L, and water temperature control is 25 DEG C, and illumination control is in 7000Lux, 8.2 ° of DH of water body hardness, water velocity
3m/min；When hatching was by 2 hours, embryonated egg is put into 0 DEG C, 4min in the cold water added with herbal mediciment, herbal mediciment is extracted
The addition of thing is 80g/ jins of fish weights, and herbal mediciment extract is prepared from by the raw material of following parts by weight：30 parts of taxol, dragon
10 parts of certain herbaceous plants with big flowers total alkali, 10 parts of angelica extract, 8 parts of Astragalus Root P.E, 0.01 part of light rare earth, cold dissolved oxygen in water is maintained at 8.0mg/L
More than, suppress its chromosome separation, it is smoothly doubled fusion, form tetraploid；Then take out the water body once again in incubator
It is middle to be hatched, until emerging grows up to spray.
Embodiment two
Gan fishes and black carp quasi aromatic hybrid rice breeding method, comprise the following steps：
（1）The selection of parent：Choose 3 more than age, body weight 7-20kg, gonadal maturation and Jian Kang Gan fishes female be as female
This；The black carp male of 4-5 ages, body weight 500-5000g, gonadal maturation and health is chosen as male parent, 12 before hybridization
Month, just allow female, male in parent independent cultivate；
（2）The reinforced cultivating of parent：By step（1）Pick out in Gan fish female parent Fang Ru Gan fish parent cultures pond, and control
Culture pond water temperature processed is 24 DEG C, starts within 3 months before hybridization to feed the fruit of Chinese magnoliavine 15%, the honeysuckle by by weight percentage
10%th, Radix Codonopsis 20%, B groups vitamin 5%, the Radix Astragali 10%, zymosan 5%, Semen Cuscutae powder 10%, eel bone meal 30%, soft-shelled turtle egg 10% are matched somebody with somebody
The bait of system（Granular bait for fish is made according to a conventional method）, day throwing amount is the tails of 2000kg/ ten thousand, promotes gonad development；It will select
The black carp male parent gone out is put into black carp parent culture pond, and controls water temperature in culture pond to be 18 DEG C；
（3）Hasten parturition：To passing through step（2）The injection of this Gan of the Mu of processing fishes is promoted by carp pituitary 0.9mg/kg fish bodies weight, injection
The mixing ocyodinic of Luteinizing hormone releasing hormone A2 60ug/kg fish bodies weight, the IU/kg fish bodies of injection chorionic gonadotrophin 3 weight；It is blue or green to male parent
Fish injects ocyodinic LRH-A 0.25ug+HCG100 IU/kg fish body weights,；
（4）Artificial insemination：Conscientious observation of steps（3）Parent, the mature egg of , Cai Gan fishes and green grass or young crops when both of which full maturity
The mature sperm of fish, essence:The volume ratio of ovum is 1:5 are sufficiently mixed, i.e., the quantity ratio of smart ovum is more than 500,000,000：1, temperature control
At 24 DEG C, time of insemination 5min is stand-by；
（5）Hatching：Take step（4）In embryonated egg, be put into incubator and carry out Lotic hatching, Spawning water pH value control exist
8.4, dissolved oxygen is 8.0mg/L, and water temperature control is 27 DEG C, and illumination control is in 20000Lux, 8.5 ° of DH of water body hardness, water velocity
5m/min；When hatching was by 2 hours, embryonated egg is put into 1 DEG C, 3min, Chinese herbal medicine in the cold water added with herbal mediciment extract
The addition of agent extract is 80g/ jins of fish weights, and herbal mediciment extract is prepared from by the raw material of following parts by weight：Taxol
20 parts, 15 parts of nightshade total alkali, 10 parts of angelica extract, 10 parts of Astragalus Root P.E, 0.1 part, cold dissolved oxygen in water is maintained at 5.0mg/L
More than, cold dissolved oxygen in water is maintained at more than 5.0mg/L, suppresses its chromosome separation, it is smoothly doubled fusion, and shape quadruples
Body；Then take out and hatched once again in the water body of incubator, until emerging grows up to spray.
Inventor's the method according to the invention is obtained to continue to track after spray and fed to 3 ages, the speed of growth between Bambusa fishes with
Between black carp.Its survival rate and trophic analysis are counted, specific data refer to following table one.
Table one：Hybridization fish is compared with parent
| Kind name | Survival rate | Feeding habits |
| The Hybrid of the present invention | 80.15-90.10% | Omnivorousness, meat and vegetables |
| Gan fishes | 65.82%-71.23% | Grazing eclipse, carnivority |
| Black carp | 75.14%-84.37% | Omnivorousness, staple food grain bait |
It was found from table one, strain fish of the invention is more preferably fed, and feeding habits are wide, and survival rate Xiang increases Bi Gan fish, black carp, value
It must widely popularize.
Claims (2)
1. Yi Zhong Gan fishes and black carp quasi aromatic hybrid rice breeding method, by the selection of parent, the reinforced cultivating of parent, hasten parturition, people
Work is inseminated, hatches these steps to realize, it is characterised in that
The reinforced cultivating concrete operations of above-mentioned parent are：It will be singled out in Gan fish female parent Fang Ru Gan fish parent cultures pond,
And control culture pond water temperature to be 20-24 DEG C, start within 2-3 months before hybridization to feed including fruit of Chinese magnoliavine 8-15%, honeysuckle 5-10%,
Radix Codonopsis 10-20%, B group vitamin 2-5%, Radix Astragali 5-10%, zymosan 3-5%, Semen Cuscutae powder 5-10%, eel bone meal 10-30%, first
Bait including fish egg 5-10%, day throwing amount is the tails of 1000-2000kg/ ten thousand, promotes gonad development；The black carp male that will be singled out
Parent is put into black carp parent culture pond, and controls water temperature in culture pond to be 16-18 DEG C；
The concrete operations of above-mentioned artificial insemination are：Parent after conscientiously observation is hastened parturition, , Cai Gan fishes when both of which full maturity
Mature egg and the mature sperm of black carp, essence:The volume ratio of ovum is 1:3-5 is sufficiently mixed, i.e., the quantity ratio of smart ovum is more than 5
Hundred million：1, temperature control is at 22-24 DEG C, and time of insemination 3-5min is stand-by；
The concrete operations of above-mentioned hatching are：The embryonated egg of artificial insemination is taken, is put into incubator and carries out Lotic hatching, Spawning water
PH value is controlled in 8.0-8.4, and dissolved oxygen is 6.5-8.0mg/L, and water temperature control is 25-27 DEG C, and illumination is controlled in 7000-
20000Lux, 8.2-8.5 ° of DH of water body hardness, water velocity is 3-5m/min；When hatching was by 2 hours, embryonated egg is put into 0-
1 DEG C, 3-4min in the cold water added with herbal mediciment extract, cold dissolved oxygen in water are maintained at more than 5.0mg/L, suppress its chromosome
Division, makes it smoothly double fusion, forms tetraploid；Then take out and hatched once again in the water body of incubator, until going out
Seedling grows up to spray.
2. according to claim 1 Suo Shu Gan fishes and black carp quasi aromatic hybrid rice breeding method, it is characterised in that：In incubation step
Herbal mediciment is mainly prepared from by the raw material of following parts by weight：20-30 parts of taxol, 10-15 parts of nightshade total alkali, Radix Angelicae Sinensis are extracted
8-10 parts of thing, 8-10 parts of Astragalus Root P.E, 0.01-0.1 parts of light rare earth.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710477242.3A CN107155971A (en) | 2017-06-21 | 2017-06-21 | Yi Zhong Gan fishes and black carp quasi aromatic hybrid rice breeding method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710477242.3A CN107155971A (en) | 2017-06-21 | 2017-06-21 | Yi Zhong Gan fishes and black carp quasi aromatic hybrid rice breeding method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107155971A true CN107155971A (en) | 2017-09-15 |
Family
ID=59819099
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710477242.3A Pending CN107155971A (en) | 2017-06-21 | 2017-06-21 | Yi Zhong Gan fishes and black carp quasi aromatic hybrid rice breeding method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107155971A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108401951A (en) * | 2018-03-30 | 2018-08-17 | 镇沅今晨山水产科技有限公司 | A kind of artificial breeding method of Homer loach |
| CN108925472A (en) * | 2018-07-02 | 2018-12-04 | 华中农业大学 | A kind of Bambusa and megalobrama amblycephala cross breeding method |
| CN114698575A (en) * | 2022-05-10 | 2022-07-05 | 湖南文理学院 | Breeding method for variegated carp heterogenous gynogenesis |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106172117A (en) * | 2016-07-14 | 2016-12-07 | 浙江省海洋水产研究所 | Carnis Pseudosciaenae raun and Carnis Pseudosciaenae milter cross breeding method |
-
2017
- 2017-06-21 CN CN201710477242.3A patent/CN107155971A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106172117A (en) * | 2016-07-14 | 2016-12-07 | 浙江省海洋水产研究所 | Carnis Pseudosciaenae raun and Carnis Pseudosciaenae milter cross breeding method |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108401951A (en) * | 2018-03-30 | 2018-08-17 | 镇沅今晨山水产科技有限公司 | A kind of artificial breeding method of Homer loach |
| CN108925472A (en) * | 2018-07-02 | 2018-12-04 | 华中农业大学 | A kind of Bambusa and megalobrama amblycephala cross breeding method |
| CN114698575A (en) * | 2022-05-10 | 2022-07-05 | 湖南文理学院 | Breeding method for variegated carp heterogenous gynogenesis |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100464633C (en) | Erythtoculer ilishaefornis and megajobrama amblycephala crossbreeding method | |
| Xie et al. | Grass carp: the fish that feeds half of China | |
| CN106577388B (en) | A kind of cross breeding method of excellent No. 1 Pelteobagrus fulvidraco of Huang of improvement growth traits | |
| CN101836607B (en) | Method for breeding macrobrachium nipponensis | |
| CN102047851A (en) | Construction and stock breeding method for cultured grass carp families | |
| CN101103706B (en) | Reproduction method for American reeves shad artificial cultivation parent fish | |
| CN110024722A (en) | A kind of artificial fecundation method of scavenger | |
| CN106212333A (en) | A kind of artificial fecundation method of north palpus loach | |
| CN101647412B (en) | Method for breeding tridentiger trigonocephalus by artificial induced spawning and insemination | |
| CN102017921A (en) | Siniperca chuatsi and siniperca kneri hybridization and culture method | |
| CN107155971A (en) | Yi Zhong Gan fishes and black carp quasi aromatic hybrid rice breeding method | |
| CN106172136A (en) | A kind of Puffer fish artificial fecundation method in winter | |
| CN103493757A (en) | Method for breeding and cultivating four generations of fish and shrimp seedlings in pond in one year | |
| CN104542478A (en) | Imitative ecological breeding and reproduction technology of andrias davidianus | |
| CN110100770A (en) | A kind of artificial fecundation method of basket fish | |
| Hu et al. | Developments in common carp culture and selective breeding of new varieties | |
| CN105265362A (en) | Cross breeding method for improving growth traits of slender mandarinfish | |
| CN111226839B (en) | Method for breeding hypoxia-resistant hybrid pelteobagrus fulvidraco | |
| Li et al. | The success of yellow catfish aquaculture in China: From rare wild fish to popular farmed fish | |
| CN100512639C (en) | Plecoglossus altivelis artificial reproduction method | |
| CN106376500B (en) | The thin squama schizothoracin artificial fecundation of Yalongjiang River releases method | |
| CN103518649B (en) | Fresh water black porgy and Jade Perch hybridizing propagation process | |
| CN111109166B (en) | Artificial breeding method for acrossocheilus fasciatus | |
| CN110178766B (en) | Artificial breeding method of Acipenser lapipenser | |
| CN107114281A (en) | A kind of Culter Yu Yu Gan fish quasi aromatic hybrid rice breeding methods |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170915 |