CN111109166B - Artificial breeding method for acrossocheilus fasciatus - Google Patents
Artificial breeding method for acrossocheilus fasciatus Download PDFInfo
- Publication number
- CN111109166B CN111109166B CN202010168187.1A CN202010168187A CN111109166B CN 111109166 B CN111109166 B CN 111109166B CN 202010168187 A CN202010168187 A CN 202010168187A CN 111109166 B CN111109166 B CN 111109166B
- Authority
- CN
- China
- Prior art keywords
- water
- artificial
- fish
- cultivation
- feeding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000009395 breeding Methods 0.000 title claims abstract description 50
- 241001101885 Acrossocheilus fasciatus Species 0.000 title description 3
- 241000251468 Actinopterygii Species 0.000 claims abstract description 116
- 230000001488 breeding effect Effects 0.000 claims abstract description 42
- 230000009027 insemination Effects 0.000 claims abstract description 22
- 238000011534 incubation Methods 0.000 claims abstract description 21
- 230000005070 ripening Effects 0.000 claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 162
- 230000000366 juvenile effect Effects 0.000 claims description 29
- 235000013601 eggs Nutrition 0.000 claims description 28
- 238000000034 method Methods 0.000 claims description 23
- 235000019750 Crude protein Nutrition 0.000 claims description 21
- 230000003203 everyday effect Effects 0.000 claims description 20
- 230000001965 increasing effect Effects 0.000 claims description 20
- 230000006698 induction Effects 0.000 claims description 20
- 238000007667 floating Methods 0.000 claims description 19
- 241001275937 Acrossocheilus Species 0.000 claims description 17
- 230000032696 parturition Effects 0.000 claims description 17
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 16
- 229910052760 oxygen Inorganic materials 0.000 claims description 16
- 239000001301 oxygen Substances 0.000 claims description 16
- 210000000582 semen Anatomy 0.000 claims description 15
- 239000004568 cement Substances 0.000 claims description 11
- 210000002149 gonad Anatomy 0.000 claims description 11
- 210000001015 abdomen Anatomy 0.000 claims description 10
- 230000026109 gonad development Effects 0.000 claims description 10
- 229940088597 hormone Drugs 0.000 claims description 10
- 239000005556 hormone Substances 0.000 claims description 10
- 239000007924 injection Substances 0.000 claims description 10
- 238000002347 injection Methods 0.000 claims description 10
- 239000002689 soil Substances 0.000 claims description 10
- 239000008188 pellet Substances 0.000 claims description 9
- 238000004140 cleaning Methods 0.000 claims description 8
- 238000012258 culturing Methods 0.000 claims description 8
- 238000006213 oxygenation reaction Methods 0.000 claims description 8
- 230000001418 larval effect Effects 0.000 claims description 7
- 241001502127 Mullus barbatus Species 0.000 claims description 5
- XNOPRXBHLZRZKH-UHFFFAOYSA-N Oxytocin Natural products N1C(=O)C(N)CSSCC(C(=O)N2C(CCC2)C(=O)NC(CC(C)C)C(=O)NCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(CCC(N)=O)NC(=O)C(C(C)CC)NC(=O)C1CC1=CC=C(O)C=C1 XNOPRXBHLZRZKH-UHFFFAOYSA-N 0.000 claims description 5
- 101800000989 Oxytocin Proteins 0.000 claims description 5
- 102100031951 Oxytocin-neurophysin 1 Human genes 0.000 claims description 5
- 238000010521 absorption reaction Methods 0.000 claims description 5
- 230000002354 daily effect Effects 0.000 claims description 5
- 230000000249 desinfective effect Effects 0.000 claims description 5
- 238000007865 diluting Methods 0.000 claims description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 5
- 230000000694 effects Effects 0.000 claims description 5
- 210000003746 feather Anatomy 0.000 claims description 5
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 claims description 5
- 239000012528 membrane Substances 0.000 claims description 5
- XNOPRXBHLZRZKH-DSZYJQQASA-N oxytocin Chemical compound C([C@H]1C(=O)N[C@H](C(N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CSSC[C@H](N)C(=O)N1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(C)C)C(=O)NCC(N)=O)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 XNOPRXBHLZRZKH-DSZYJQQASA-N 0.000 claims description 5
- 229960001723 oxytocin Drugs 0.000 claims description 5
- 239000002504 physiological saline solution Substances 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 5
- 238000003825 pressing Methods 0.000 claims description 5
- 230000000638 stimulation Effects 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 230000009182 swimming Effects 0.000 claims description 5
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 claims description 5
- 230000006378 damage Effects 0.000 claims description 4
- 201000010099 disease Diseases 0.000 claims description 4
- 241000349701 Acrocarpus Species 0.000 claims description 3
- 102000011022 Chorionic Gonadotropin Human genes 0.000 claims description 3
- 108010062540 Chorionic Gonadotropin Proteins 0.000 claims description 3
- 108700012941 GNRH1 Proteins 0.000 claims description 3
- 239000000579 Gonadotropin-Releasing Hormone Substances 0.000 claims description 3
- 241000339989 Wolffia Species 0.000 claims description 3
- 229940015047 chorionic gonadotropin Drugs 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 2
- 238000009826 distribution Methods 0.000 abstract description 2
- 238000011031 large-scale manufacturing process Methods 0.000 abstract 1
- 239000003643 water by type Substances 0.000 abstract 1
- 210000000006 pectoral fin Anatomy 0.000 description 7
- 238000011161 development Methods 0.000 description 6
- 230000018109 developmental process Effects 0.000 description 6
- 241000252234 Hypophthalmichthys nobilis Species 0.000 description 5
- 230000012447 hatching Effects 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 4
- 241000252232 Hypophthalmichthys Species 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000004720 fertilization Effects 0.000 description 3
- 239000002434 gonadorelin derivative Substances 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 244000207740 Lemna minor Species 0.000 description 2
- 235000006439 Lemna minor Nutrition 0.000 description 2
- 241001560086 Pachyrhizus Species 0.000 description 2
- 235000001855 Portulaca oleracea Nutrition 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 230000008033 biological extinction Effects 0.000 description 2
- 239000013505 freshwater Substances 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 238000009342 intercropping Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 239000001763 2-hydroxyethyl(trimethyl)azanium Substances 0.000 description 1
- 206010000591 Acrochordon Diseases 0.000 description 1
- 240000002900 Arthrospira platensis Species 0.000 description 1
- 235000016425 Arthrospira platensis Nutrition 0.000 description 1
- 235000019743 Choline chloride Nutrition 0.000 description 1
- 241000703788 Cirrhinus molitorella Species 0.000 description 1
- 241000252210 Cyprinidae Species 0.000 description 1
- 235000019733 Fish meal Nutrition 0.000 description 1
- 241001600354 Hemibarbus Species 0.000 description 1
- 235000019779 Rapeseed Meal Nutrition 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 1
- 235000019764 Soybean Meal Nutrition 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229960003178 choline chloride Drugs 0.000 description 1
- SGMZJAMFUVOLNK-UHFFFAOYSA-M choline chloride Chemical compound [Cl-].C[N+](C)(C)CCO SGMZJAMFUVOLNK-UHFFFAOYSA-M 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000003750 conditioning effect Effects 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000004467 fishmeal Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 238000012214 genetic breeding Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910000150 monocalcium phosphate Inorganic materials 0.000 description 1
- 235000019691 monocalcium phosphate Nutrition 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000001007 puffing effect Effects 0.000 description 1
- 239000004456 rapeseed meal Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 201000000195 skin tag Diseases 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000004455 soybean meal Substances 0.000 description 1
- 235000012424 soybean oil Nutrition 0.000 description 1
- 239000003549 soybean oil Substances 0.000 description 1
- 229940082787 spirulina Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a thick lip fish (A)Acrossocheilius parallens) Artificial breeding method, which is to be captured from the fieldThe full-artificial breeding of the thick lipped lateral is realized by the steps of parent daily breeding, prenatal ripening, artificial spawning, artificial insemination, artificial incubation, fry breeding and the like. The invention provides a mode suitable for large-scale production of lateral thick lipped fry, breaks through the technical bottleneck of artificial breeding of lateral thick lipped fry, realizes the full-artificial breeding of the lateral thick lipped fry at home and abroad for the first time, provides technical support and guarantee for the germplasm resource conservation area of the thick lipped fry in national level aquatic products and other waters with natural population distribution, and lays a good foundation for the large-scale breeding, artificial breeding and other works of the lateral thick lipped fry.
Description
Technical Field
The invention belongs to the field of aquaculture, and particularly relates to an artificial breeding method of acrossocheilus laterosporus.
Background
The landform of China is in various states, the inland drainage basin is wide in area, and the method has rich local freshwater fish resources, wherein the variety with development and utilization values is more. The local characteristic fish resources have important ecological value and genetic breeding value, and have the characteristics of rich nutrition, delicious meat quality, unique flavor and the like. The aquatic germplasm resources are important material bases for aquatic breeding, aquaculture production, resource restoration and fishery scientific and technological development. Although protection, development and utilization works such as resource investigation, variety identification, seedling breeding, artificial domestication and the like are initially carried out on part of varieties at present, most varieties are still in the stage of to-be-researched and developed, the related research works progress slowly, and the foundation is relatively weak.
The thick lipped lateral is a small economic fish of the genus Acrossocheilus of the family Cyprinidae, and is a species specific to China; a blue-black longitudinal zone extending through the caudate base along the measuring line is an important morphological feature of the aphanidermia which is different from other fishes of the aphanidermia. The thick lipped lateral strip is a main protective species of the national aquatic germplasm resource protection region of the thick lipped fish of the Songxi river (approved and established by the Min's department of agriculture 2016), has high ecological value and economic value, is common edible fish in the mountainous regions of Jiangxing and Guangdong, is deeply favored by common people, and has wide market prospect.
The population quantity and quality of the acrossocheilus lateroscheilus are seriously exhausted due to the damage to the ecological environment of the original producing area, the capture in disorder, the water environment pollution and the like, and the risk of extinction of wild germplasm resources exists. In view of this, research works such as germplasm resource protection, artificial breeding, cultivation and development and the like need to be carried out on the variety to realize the recovery and continuation of germplasm resources and meet market demands. Related research data related to the acrossocheilus laterosporus is few, and researches in the aspects of breeding biology, nutriology, disease control technology and the like are basically blank; at present, the success of artificial breeding is not reported at home and abroad. In view of the above, the invention adopts the initiated prenatal cultivation, ripening and artificial spawning method, successfully realizes the artificial breeding of the acrochordon pachychia baronii for the first time at home and abroad, and lays a solid foundation for the research and development work of the fry scale production, germplasm resource protection, cultivation and development application and the like of the fish.
Disclosure of Invention
The invention provides an artificial breeding method of acrocarpus pachyrhizus, aiming at the current situation that the existing artificial breeding technology of the acrocarpus pachyrhizus is lack.
In order to achieve the purpose, the invention adopts the following technical scheme:
an artificial breeding method of acrossocheilus lateral banding comprises the following specific steps:
(1) parent breeding:
a) and (3) daily cultivation: using 2000 m2Cultivating wild lateral striped mullet parents in a soil pond with the depth of water within 1.2-1.6 m, wherein the stocking density is 4000-5000 tails/mu; intercropping 100-150 silver carps and 30-50 bighead carps per mu to regulate and control water quality; feeding floating granulated feed with crude protein content of 36-40%, wherein the feeding frequency is 2 times/d, and the feeding rate is 1-2%; maintaining micro-flowing water, and providing an oxygen increasing facility to ensure that the dissolved oxygen of the cultivation water body reaches more than 5 mg/L, the pH value is 7.0-8.5, and the transparency is more than 30 cm;
b) pre-partum cultivation: selecting individuals without diseases and injuries and with good gonad development in batches from a soil pond for breeding parent fishes at the end of 3-4 months every year, and putting the individuals into a container with the length of 30 m2～50 m2A depth of water of 0.5 to 0.6 mCarrying out prenatal intensified cultivation in a shaped cement pond (provided with an oxygenation facility), and putting 300-400 fish parents into each pond to keep running water stimulation; from 10-15 days before induction of labor, reducing the water level by 5-6 cm every 3 days until the water depth reaches 0.3-0.4 m; meanwhile, the water inflow and drainage amount is increased, so that the water inflow and drainage amount is 60-80 tons every day, and the gonads of the parent fishes are stimulated to rapidly and synchronously develop; feeding floating granulated feed with crude protein content of 36-38%, wherein the feeding frequency is 2 times/d, and the feeding rate is 1-2%; feeding green feed wolffia for 1-2 times every week;
c) pre-parturition ripening: before 6-7 days of induction, the measures for increasing the water intake and drainage are continuously adopted to stimulate the gonad development of the parent fish, and the method of artificial hormone intervention is also adopted to carry out the prenatal ripening of the female fish (the male fish is not ripened); the artificial hormone is 2 mg luteinizing hormone releasing hormone analog (LRH-A)2) Or 1 mg of diutanone maleate (DOM) + 1 mg of luteinizing hormone releasing hormone analog (LRH-A)2) The mixed preparation adopts a primary injection method at the base of the pectoral fin;
in the operation of the step (1), the inlet water is filtered by a 80-100-mesh bolting silk bag;
(2) artificial hastening parturition: selecting individuals with mature gonads from parent fishes subjected to prenatal intensive cultivation and ripening for artificial spawning induction, wherein the ratio of male to female is 5-8: 1; the oxytocin for female fish is 1000 IU chorionic gonadotropin (HCG) +6 mg diutanone maleate (DOM) +6 mg luteinizing hormone releasing hormone analog (LRH-A)2) The dosage of the male fish is reduced by half, and a one-time injection method is adopted at the base part of the pectoral fin; hasten parturition at the water temperature of 25-27 ℃ for 12-14 h;
(3) artificial insemination: adopts a dry insemination mode to perform insemination, and comprises the following specific operation steps: wiping off water on the abdomen of the female fish in the effect period, and slightly pressing the abdomen of the female fish to extrude eggs into a clean and dry container; meanwhile, diluting the semen extruded from the male fish with 1-2 times of volume of physiological saline (the concentration is 0.7 percent and the pH is 6.8-7.0), immediately pouring the diluted semen into the just extruded eggs, immediately and gently stirring the diluted semen with feathers for 1-2 min for artificial insemination, then cleaning the fertilized eggs with clear water and then hatching;
(4) artificial incubation: uniformly arranging the fertilized eggs after water absorption and expansion on an incubation device (ZL 201621366110.0) and adopting a micro-flowing water incubation mode; the dissolved oxygen of the water body is ensured to be more than 5 mg/L during incubation; incubating at the water temperature of 25-27 ℃, and taking the larval fish out of the membrane after about 55-60 h;
(5) and (3) seedling cultivation:
a) fry breeding: using 50 m2～100 m2The cement pond is used as a seedling culture pond and is provided with an oxygenation facility; cleaning and disinfecting the seedling culture pond before the water flower seedlings come out of the film, and adding clear water with the height of 20-30 cm every other day; the overflow holes are bound by a 100-mesh bolting silk net bag; the stocking density of the water flower seedlings is 800-1000 tails/m2(ii) a Feeding zooplankton sieved by 80 meshes after the flat swimming of the fry, wherein the abundance of the bait is 8-10 per meter2(ii) a Adding water for 4-5 cm every day from the opening of the larval fish, and adding water from 20-30 cm to 50-60 cm without changing water;
b) culturing the juvenile fishes: feeding the juvenile fishes of 12-30 days old with 60-mesh sieved zooplankton, wherein the abundance of the bait is 5-8 per m2(ii) a The juvenile fish culture density is 600-800 tails/m2(ii) a In the stage, the cultivation water level is kept at 50-60 cm, and 8-10 cm of water is changed every day;
feeding floating pellet feed with crude protein content of 40% -42% for domesticating young fishes at the age of 22 days, wherein the feeding frequency is 4-5 times/d, and the feeding rate is 6-8%; continuously keeping the cultivation water level of 50-60 cm at the stage, and changing water 10-20 cm every day;
c) and (3) juvenile fish cultivation: feeding floating pellet feed with 38% -40% of crude protein content from 30 days old, wherein the feeding frequency is 3-4 times/d, and the feeding rate is 4-6%; the juvenile fish culture density is 400-600 tails/m2(ii) a In the stage, the water level for cultivation is increased to 70-80 cm, and micro-flowing water is kept; culturing for 41-55 days to obtain juvenile fish fries with the specification of more than 3 cm;
and (5) filtering the inlet water by using a 60-80-mesh bolting silk bag in the operation of the step (5).
The invention has the beneficial effects that:
the acrossocheilus fasciatus is a unique protected species in China and has important ecological value and economic value. In recent years, due to the facts that the ecological environment of a production place is damaged, is captured disorderly, is polluted by a water environment and the like, the population quantity and the quality of the wild germplasm resources are seriously exhausted, and the wild germplasm resources are at risk of extinction. The invention adopts the original prenatal cultivation, ripening and artificial spawning method to realize the artificial breeding of the acrossocheilus laterosporus for the first time at home and abroad; by the prenatal ripening method, the maturity and the development synchronism of the gonads of the female fishes can be improved, and the ripening rate and the artificial breeding success rate of the thick lipped hemibarbus lateral are further improved. The method can provide technical support and seed guarantee for the resource restoration of the national-level aquatic germplasm resource protection area of the acrossocheilus crassipes and other water areas with natural population distribution, and lays a good foundation for the large-scale seedling culture and artificial culture of the acrossocheilus crassipes.
Detailed Description
In order to make the present invention more comprehensible, the technical solutions of the present invention are further described below with reference to specific embodiments, but the present invention is not limited thereto.
The buoyant pellet feed with the crude protein content of 36% -42% is prepared by mixing and blending 25-35 parts by weight of fish meal, 20-23 parts by weight of flour, 8-15 parts by weight of corn protein powder, 10-16 parts by weight of soybean meal, 7-13 parts by weight of rapeseed meal, 2-5 parts by weight of soybean oil, 4-6 parts by weight of beer yeast, 0.2-0.5 part by weight of multivitamin, 1.0-1.5 parts by weight of compound mineral, 0.2-0.5 part by weight of choline chloride, 1-2 parts by weight of monocalcium phosphate and 0.5-0.8 part by weight of spirulina powder, and then carrying out steam conditioning, puffing, granulating, drying and the like.
Example 1
In 6 months in 2017, in a fish breeding farm of McGastridae fingerling raising Co., Ltd, Shunchang county, Fujian province, the method is used for breeding the wild parent of the acrossocheilus laterosporus, the key technology of artificial breeding of the wild parent is broken through, and the artificial breeding of the acrossocheilus laterosporus is realized for the first time. 3 batches of spawning induction are carried out for 3 times, the spawning induction of the female fish is total 109 tails, the spawning induction is successful 64 tails, and the average spawning rate is 58.7%; 5.13 ten thousand eggs and 3.44 ten thousand fertilized eggs are obtained, and the average fertilization rate is 67.1 percent; 2.43 thousands of seedlings were hatched, and the average hatchability was 70.6%. After 45-55 d cultivation, 1.79 thousands of summer fingerlings of the acrossocheilus laterosporus with the average specification of more than 3.0 cm are obtained, and the average survival rate of fingerling cultivation reaches 73.7%.
The specific breeding steps are as follows:
(1) parent breeding:
a) and (3) daily cultivation: using 1000 m2Cultivating wild lateral striped mullet parents in a soil pond with the water depth of 1.2-1.4 m, wherein the stocking density is 5000 tails/mu; 1, intercropping 100 silver carps and 30 bighead carps per mu to regulate and control water quality; feeding floating granulated feed with 36 percent of crude protein content, wherein the feeding frequency is 2 times/d, and the feeding rate is 1 to 2 percent; maintaining micro-flowing water, and providing an oxygen increasing facility to ensure that the dissolved oxygen of the cultivation water body reaches more than 5 mg/L, the pH value is 7.0-8.5, and the transparency is more than 30 cm;
b) pre-partum cultivation: selecting individuals with good gonad development and no disease or injury from soil pond for breeding parent fish in batches at the beginning of 4 months, and placing into 30 m2Carrying out prenatal intensified cultivation in rectangular cement ponds (provided with oxygenation facilities) with the water depth of 0.5 m, and putting 300 fish of parent fish in each pond to keep running water stimulation; from 10 days before induction, the water level is reduced by 5 cm every 3 days until the water depth reaches 0.4 m; simultaneously, water inflow and drainage are increased, so that the water inflow and drainage are 60 tons every day, and the gonads of the parent fishes are stimulated to rapidly and synchronously develop; feeding floating granulated feed with 36 percent of crude protein content, wherein the feeding frequency is 2 times/d, and the feeding rate is 1 to 2 percent; feeding green feed wolffia for 1 time every week;
c) pre-parturition ripening: in addition to continuously adopting the measure of increasing water inflow and drainage quantity to stimulate the gonad development of the parent fish at 6 days before the spawning promotion, the method of artificial hormone intervention is also adopted to carry out the pre-spawning maturation promotion on the female fish (the male fish is not mature); the artificial hormone is 1 mg DOM + 1 mg LRH-A2The mixed preparation adopts a primary injection method at the base of the pectoral fin;
in the operation of the step (1), the inlet water is filtered by a 100-mesh bolting-silk bag;
(2) artificial hastening parturition: selecting individuals with mature gonads from parent fishes subjected to prenatal intensive cultivation and ripening for artificial spawning induction, wherein the ratio of male to female is 7-8: 1; the oxytocin for female fish is 1000 IU HCG +6 mg DOM +6 mg LRH-A2The dosage of the male fish is reduced by half, and a one-time injection method is adopted at the base part of the pectoral fin; hasten parturition at the water temperature of 25 ℃ for 14 h;
(3) artificial insemination: adopts a dry insemination mode to perform insemination, and comprises the following specific operation steps: wiping off water on the abdomen of the female fish in the effect period, and slightly pressing the abdomen of the female fish to extrude eggs into a clean and dry container; meanwhile, diluting the semen extruded from the male fish with 2 times of volume of physiological saline (the concentration is 0.7 percent and the pH is 6.8-7.0), immediately pouring the diluted semen into the eggs which are just extruded, immediately stirring the diluted semen for 1 min with feathers for artificial insemination, then washing the fertilized eggs with clear water and then incubating the fertilized eggs;
(4) artificial incubation: uniformly arranging the fertilized eggs after water absorption and expansion on an incubation device (ZL 201621366110.0) and adopting a micro-flowing water incubation mode; the dissolved oxygen of the water body is ensured to be more than 5 mg/L during incubation; incubating at the water temperature of 25 ℃, and enabling the larva fishes to come out of the membranes after 58-60 hours;
(5) and (3) seedling cultivation:
a) fry breeding: using 50 m2The cement pond is used as a seedling culture pond and is provided with an oxygenation facility; cleaning and disinfecting the seedling culture pond before the water flower seedlings come out of the film, and adding clear water with the height of 20 cm every other day; the overflow holes are bound by a 100-mesh bolting silk net bag; the stocking density of the water flower seedlings is 1000 tails/m2(ii) a Feeding zooplankton sieved by 80 meshes after the flat swimming of the fry, wherein the abundance of the bait is 8-9/m2(ii) a Adding water 4 cm from the opening of the fry every day to make the water level increase from 20 cm to 50 cm, and not changing water;
b) culturing the juvenile fishes: feeding the juvenile fishes of 12-22 days old with 60-mesh sieve zooplankton, wherein the abundance of the bait is 5-6 per m2(ii) a The young fish culture density is 800 tails/m2(ii) a In the stage, the water level is kept at 50 cm, and the water is changed by 8 cm every day;
feeding floating pellet feed with crude protein content of 40% for domesticating young fishes at 22 days, wherein the feeding frequency is 4 times/d, and the feeding rate is 6%; the stage continuously maintains the cultivation water level of 50 cm, and water is changed by 10 cm every day;
c) and (3) juvenile fish cultivation: feeding floating granulated feed with 38 percent of crude protein content from 30 days old, wherein the feeding frequency is 3 times/d, and the feeding rate is 4 percent; the juvenile fish culture density is 600 tails/m2(ii) a In the stage, the water level for cultivation is increased to 70 cm, and micro-flowing water is kept; passing through 45-55d, cultivating to obtain juvenile fish fries with the specification of more than 3 cm;
in the operation of the step (5), the inlet water is filtered by a 60-mesh bolting silk bag.
Under the same conditions, the group of breeding parents without prenatal ripening was used as a comparison, and the results are shown in Table 1.
TABLE 1
As can be seen from Table 1, the induction of the spawning rate of the acrossocheilus wisonii can be obviously improved by accelerating the ripening before the spawning, and the success rate of artificial breeding is improved.
Example 2
In 2018, 5-6 months, artificial breeding production work of the wild parent of the acrossocheilus laterosporus is developed by applying the method of the invention again in a fry breeding cooperative base of the fresh water aquatic research institute in Fujian province. 5 batches of spawning induction are performed in total, the female fish 233 tails are spawned together, the spawning induction is successful, and the average spawning rate reaches 72.5 percent; obtaining 13.32 ten thousand eggs and 9.24 ten thousand fertilized eggs, wherein the average fertilization rate is 69.4%; 7.17 thousands of hatched seedlings, and the average hatching rate is 77.6 percent. 5.48 thousands of summer fingerlings of the acrossocheilus laterosporus with the average specification of more than 3.0 cm are obtained after cultivation for 42-51 days, and the average survival rate of the fingerlings reaches 76.4%. The specific breeding steps are as follows:
(1) parent breeding:
a) and (3) daily cultivation: using 1667 m2Cultivating wild lateral striped mullet parents in a soil pond with the water depth of 1.3-1.6 m, wherein the stocking density is 4000 tails/mu; culturing silver carp 120/mu and bighead carp 40/mu in a mixed mode to regulate and control water quality; feeding floating granulated feed with 38 percent of crude protein content, wherein the feeding frequency is 2 times/d, and the feeding rate is 1 to 2 percent; maintaining micro-flowing water, and providing an oxygen increasing facility to ensure that the dissolved oxygen of the cultivation water body reaches more than 5 mg/L, the pH value is 7.0-8.5, and the transparency is more than 30 cm;
b) pre-partum cultivation: selecting individuals with good gonad development in batches from a soil pond for breeding parent fish at the end of 3 months, and putting the individuals into a place with a length of 40 m2In a rectangular cement pool (equipped with an oxygen increasing facility) with the water depth of 0.5 mCarrying out prenatal intensive cultivation, putting 350 tails of parent fishes in each pond, and keeping running water stimulation; from 12 d before induction, the water level is reduced by 6 cm every 3 d until the water depth reaches 0.3 m; meanwhile, the water inflow and drainage amount is increased, so that the water inflow and drainage amount is 70 tons every day, and the gonads of the parent fishes are stimulated to rapidly and synchronously develop; feeding floating granulated feed with 38 percent of crude protein content, wherein the feeding frequency is 2 times/d, and the feeding rate is 1 to 2 percent; green feed duckweed is fed for 2 times every week;
c) pre-parturition ripening: in addition to continuously adopting the measure of increasing water inflow and drainage quantity to stimulate the gonad development of the parent fish at 7 days before the induction of parturition, the antenatal ripening of the female fish is also carried out by adopting a method of artificial hormone intervention (the male fish is not induced to ripen); the artificial hormone is 2 mg LRH-A2Adopting a primary injection method at the base of the pectoral fin;
in the operation of the step (1), the inlet water is filtered by a 100-mesh bolting-silk bag;
(2) artificial hastening parturition: selecting individuals with mature gonads from parent fishes subjected to prenatal intensive cultivation and ripening for artificial spawning induction, wherein the ratio of male to female is 6-7: 1; the oxytocin for female fish is 1000 HCG +6 mg DOM +6 mg LRH-A2The dosage of the male fish is reduced by half, and a one-time injection method is adopted at the base part of the pectoral fin; hasten parturition at the water temperature of 26-27 ℃ for 13-14 h;
(3) artificial insemination: adopts a dry insemination mode to perform insemination, and comprises the following specific operation steps: wiping off water on the abdomen of the female fish in the effect period, and slightly pressing the abdomen of the female fish to extrude eggs into a clean and dry container; meanwhile, diluting the semen extruded from the male fish with 1 time volume of physiological saline (the concentration is 0.7 percent and the pH is 6.8-7.0), immediately pouring the diluted semen into the eggs which are just extruded, immediately stirring the diluted semen for 2 min with feathers for artificial insemination, then washing the fertilized eggs with clear water and then incubating the fertilized eggs;
(4) artificial incubation: uniformly arranging the fertilized eggs after water absorption and expansion on an incubation device (ZL 201621366110.0) and adopting a micro-flowing water incubation mode; the dissolved oxygen of the water body is ensured to be more than 5 mg/L during incubation; incubating at the water temperature of 26-27 ℃, and taking the larval fish out of the membrane after about 55-58 h;
(5) and (3) seedling cultivation:
a) fry cultureBreeding: using a 70 m2The cement pond is used as a seedling culture pond and is provided with an oxygenation facility; cleaning and disinfecting the seedling culture pond before the water flower seedlings come out of the film, and adding clear water with the height of 20-25 cm every other day; the overflow holes are bound by a 100-mesh bolting silk net bag; the stocking density of the water flower seedlings is 900 tails/m2(ii) a Feeding zooplankton sieved by 80 meshes after the flat swimming of the fry, wherein the abundance of the bait is 9-10 per meter2(ii) a Adding water for 5 cm every day from the opening of the larval fish, and adding the water from 20-25 cm to 50-55 cm without changing the water;
b) culturing the juvenile fishes: feeding juvenile fish of 15-25 days old with 60-mesh sieve zooplankton, wherein the abundance of the bait is 7-8 per m2(ii) a The juvenile fish culture density is 700 tails/m2(ii) a In the stage, the cultivation water level is kept at 50-55 cm, and water is changed by 10 cm every day;
feeding floating pellet feed with 42 percent of crude protein content for domesticating young fishes at the age of 22 days, wherein the feeding frequency is 5 times/d, and the feeding rate is 7 percent; in the stage, the cultivation water level of 55 cm is kept continuously, and water is changed by 10-15 cm every day;
c) and (3) juvenile fish cultivation: feeding floating granulated feed with crude protein content of 40% from 30 days old, wherein the feeding frequency is 4 times/d, and the feeding rate is 5%; the juvenile fish culture density is 500 tails/m2(ii) a In the stage, the water level of the cultivation is increased to 80 cm, and micro-flowing water is kept; culturing for 42-51 days to obtain juvenile fish fries with the specification of more than 3 cm;
in the operation of the step (5), the inlet water is filtered by a 80-mesh bolting silk bag.
Example 3
In 2019, 5-7 months, the method is applied to cultivate the wild parent of the acrossocheilus laterosporus in the Luojia province thick-lipped fry breeding base (Jizhou base of McGao breed Limited company in Shunchang county of Fujia province), so that the artificial batch seedling culture of the wild parent is realized. Accumulating 6 batches of induced spawning, totaling 582 tails of female fishes induced spawning, successfully inducing the spawning by 467 tails, and ensuring the average induced spawning rate to be 80.2 percent; 36.64 ten thousand eggs and 27.15 ten thousand fertilized eggs are obtained, and the average fertilization rate is 74.1 percent; the hatching water seedlings have 19.85 thousands of seedlings and the average hatching rate is 73.1 percent. After 41-53 days of cultivation, 16.54 thousands of summer fingerlings of the acrossocheilus laterosporus with the average specification of more than 3.0 cm are obtained, and the average survival rate of the fingerlings reaches 83.3%. The specific breeding steps are as follows:
1) parent breeding:
a) and (3) daily cultivation: using 1667 m2Cultivating wild lateral striped mullet parents in a soil pond with the depth of 1.5-1.6 m within, wherein the stocking density is 4500 fish/mu; 150 silver carps/mu and 50 bighead carps/mu are intercropped to regulate and control water quality; feeding buoyant pellet feed with crude protein content of 40%, wherein the feeding frequency is 2 times/d, and the feeding rate is 1% -2%; maintaining micro-flowing water, and providing an oxygen increasing facility to ensure that the dissolved oxygen of the cultivation water body reaches more than 5 mg/L, the pH value is 7.0-8.5, and the transparency is more than 30 cm;
b) pre-partum cultivation: selecting individuals with good gonad development in batches from a soil pond for breeding parent fish at the end of 3 months, and putting the individuals into a 50 m pond2Carrying out prenatal intensified cultivation in rectangular cement ponds (provided with oxygenation facilities) with the water depth of 0.6 m, and putting 400 tails of parent fishes in each pond to keep running water stimulation; starting at 15 d before induction, reducing the water level by 5 cm every 3 d until the water depth reaches 0.3 m; meanwhile, the water inflow and drainage amount is increased, so that the water inflow and drainage amount is 80 tons every day, and the gonads of the parent fishes are stimulated to rapidly and synchronously develop; feeding floating granulated feed with 38 percent of crude protein content, wherein the feeding frequency is 2 times/d, and the feeding rate is 1 to 2 percent; green feed duckweed is fed for 2 times every week;
c) pre-parturition ripening: in addition to continuously adopting the measure of increasing water inflow and drainage quantity to stimulate the gonad development of the parent fish at 6 days before the induction of parturition, the antenatal ripening of the female fish is also carried out by adopting a method of artificial hormone intervention (the male fish is not ripened); the artificial hormone is 2 mg LRH-A2Adopting a primary injection method at the base of the pectoral fin;
in the operation of the step (1), the inlet water is filtered by a 100-mesh bolting-silk bag;
(2) artificial hastening parturition: selecting individuals with mature gonads from parent fishes subjected to prenatal intensive cultivation and ripening for artificial spawning induction, wherein the ratio of male to female is 5-6: 1; the oxytocin for female fish is a mixed preparation of 1000 IU chorionic gonadotropin, 6 mg diutanone maleate and 6 mg luteinizing hormone releasing hormone analogue, the dosage of male fish is halved, and a chest fin base part one-time injection method is adopted; hasten parturition at the water temperature of 25-26 ℃ for 12-13 h;
(3) artificial insemination: adopts a dry insemination mode to perform insemination, and comprises the following specific operation steps: wiping off water on the abdomen of the female fish in the effect period, and slightly pressing the abdomen of the female fish to extrude eggs into a clean and dry container; meanwhile, diluting the semen extruded from the male fish with 2 times of volume of physiological saline, immediately pouring the diluted semen into the eggs just extruded, immediately and gently stirring the diluted semen for 2 min with feathers for artificial insemination, then cleaning the fertilized eggs with clear water and then hatching;
(4) artificial incubation: uniformly arranging the fertilized eggs after water absorption and expansion on an incubation device (ZL 201621366110.0) and adopting a micro-flowing water incubation mode; the dissolved oxygen of the water body is ensured to be more than 5 mg/L during incubation; incubating at the water temperature of 25-26 ℃, and taking out the larvae from the membrane after about 57-60 h;
(5) and (3) seedling cultivation:
a) fry breeding: using 100 m2The cement pond is used as a seedling culture pond and is provided with an oxygenation facility; cleaning and disinfecting the seedling culture pond before the water flower seedlings come out of the film, and adding clear water with the height of 25-30 cm every other day; the overflow holes are bound by a 100-mesh bolting silk net bag; the stocking density of the water flower seedlings is 800 tails/m2(ii) a Feeding zooplankton sieved by 80 meshes after the flat swimming of the fry, wherein the abundance of the bait is 9-10 per meter2(ii) a Adding water for 5 cm every day from the opening of the larval fish, and adding the water from 25-30 cm to 55-60 cm without changing the water;
b) culturing the juvenile fishes: feeding the juvenile fish of 15-30 days old with 60-mesh sieve zooplankton, wherein the abundance of the bait is 7-8 per m2(ii) a The juvenile fish culture density is 600 tails/m2(ii) a In the stage, the cultivation water level is kept at 55-60 cm, and water is changed by 10 cm every day;
feeding floating granulated feed with 42 percent of crude protein content for domesticating young fishes at the age of 22 days, wherein the feeding frequency is 5 times per day, and the feeding rate is 8 percent; in the stage, the cultivation water level of 55-60 cm is kept continuously, and the water is changed by 15-20 cm every day;
c) and (3) juvenile fish cultivation: feeding floating pellet feed with crude protein content of 40% from 30 days old, wherein the feeding frequency is 4 times/d, and the feeding rate is 6%; the juvenile fish culture density is 400 tails/m2(ii) a In the stage, the water level of the cultivation is increased to 80 cm, and micro-flowing water is kept; after 41-53 d of cultivation, the product is obtainedObtaining juvenile fish fries with the specification of more than 3 cm;
in the operation of the step (5), the inlet water is filtered by a 80-mesh bolting silk bag.
The above description is only a preferred embodiment of the present invention, and all equivalent changes and modifications made in accordance with the claims of the present invention should be covered by the present invention.
Claims (2)
1. An artificial breeding method of acrossocheilus lateral stripius is characterized by comprising the following specific steps:
(1) parent breeding:
a) and (3) daily cultivation: using 2000 m2Cultivating wild lateral striped mullet parents in a soil pond with the depth of water within 1.2-1.6 m, wherein the stocking density is 4000-5000 tails/mu; feeding floating granulated feed with crude protein content of 36-40%, wherein the feeding frequency is 2 times/d, and the feeding rate is 1-2%; maintaining micro-flowing water, and providing an oxygen increasing facility to ensure that the dissolved oxygen of the cultivation water body reaches more than 5 mg/L, the pH value is 7.0-8.5, and the transparency is more than 30 cm;
b) pre-partum cultivation: at the end of 3 months to 4 months in each year, selecting individuals without diseases and injuries and with good gonad development in batches from a soil pond for breeding parents, and putting the individuals into a container with the length of 30 m2～50 m2Carrying out prenatal intensified cultivation in rectangular cement ponds with the water depth of 0.5-0.6 m, and putting 300-400 fish parents in each cement pond to keep flowing water stimulation; from 10-15 days before induction of labor, reducing the water level by 5-6 cm every 3 days until the water depth reaches 0.3-0.4 m; meanwhile, the water inflow and drainage amount is increased, so that the water inflow and drainage amount is 60-80 tons every day, and the gonads of the parent fishes are stimulated to rapidly and synchronously develop; feeding floating granulated feed with crude protein content of 36-38%, wherein the feeding frequency is 2 times/d, and the feeding rate is 1-2%; feeding green feed wolffia for 1-2 times every week;
c) pre-parturition ripening: before 6-7 d of induction, measures for increasing water inflow and drainage are continuously adopted to stimulate gonad development of the parent fish, and a method of artificial hormone intervention is also adopted to carry out antepartum ripening on the female fish; the artificial hormone is a mixed preparation of 1 mg of diutanone maleate and 1 mg of luteinizing hormone releasing hormone analogue, and a chest fin base part one-time injection method is adopted;
in the operation of the step (1), the inlet water is filtered by a 80-100-mesh bolting silk bag;
(2) artificial hastening parturition: selecting individuals with mature gonads from parent fishes subjected to prenatal intensive cultivation and ripening for artificial spawning induction, wherein the ratio of male to female is 5-8: 1; the oxytocin for female fish is a mixed preparation of 1000 IU chorionic gonadotropin, 6 mg diutanone maleate and 6 mg luteinizing hormone releasing hormone analogue, the dosage of male fish is halved, and a chest fin base part one-time injection method is adopted; hasten parturition at the water temperature of 25-27 ℃ for 12-14 h;
(3) artificial insemination: insemination is carried out by adopting a dry insemination mode;
(4) artificial incubation: uniformly arranging the fertilized eggs after water absorption and expansion on an incubation device, and adopting a microflow water incubation mode; the dissolved oxygen of the water body is ensured to be more than 5 mg/L during incubation; incubating at the water temperature of 25-27 ℃, and taking the larval fish out of the membrane after 55-60 h;
(5) and (3) seedling cultivation:
a) fry breeding: using 50 m2～100 m2The cement pond is used as a seedling culture pond and is provided with an oxygenation facility; cleaning and disinfecting the seedling culture pond before the water flower seedlings come out of the film, and adding clear water with the depth of 20-30 cm every other day; the overflow holes are bound by a 100-mesh bolting silk net bag; the stocking density of the water flower seedlings is 800-1000 tails/m2(ii) a Feeding zooplankton sieved by 80 meshes after the flat swimming of the fry, wherein the abundance of the bait is 8-10 per meter2(ii) a Adding water for 4-5 cm every day from the opening of the larval fish, and adding water from 20-30 cm to 50-60 cm without changing water;
b) culturing the juvenile fishes: feeding the juvenile fishes of 12-30 days old with 60-mesh sieved zooplankton, wherein the abundance of the bait is 5-8 per m2(ii) a The juvenile fish culture density is 600-800 tails/m2(ii) a Maintaining a cultivation water level of 50-60 cm, and changing water 8-10 cm every day;
feeding floating pellet feed with crude protein content of 40% -42% for domesticating young fishes at the age of 22 days, wherein the feeding frequency is 4-5 times/d, and the feeding rate is 6-8%; continuously keeping the cultivation water level of 50-60 cm, and changing water 10-20 cm every day;
c) and (3) juvenile fish cultivation: the content of the crude protein of the domesticated and fed feed is 38 to 40 percent from 30 days old% of floating pellet feed, the feeding frequency is 3-4 times/d, and the feeding rate is 4-6%; the juvenile fish culture density is 400-600 tails/m2(ii) a Raising the cultivation water level to 70-80 cm, and keeping micro-flowing water; obtaining juvenile fish fries with the specification of more than 3 cm through cultivation for 41-55 days;
and (5) filtering the inlet water by using a 60-80-mesh bolting silk bag in the operation of the step (5).
2. The artificial breeding method of acrocarpus pachychizus as claimed in claim 1, wherein the specific operation steps of the dry insemination in step (3) are as follows: wiping off water on the abdomen of the female fish in the effect period, and slightly pressing the abdomen of the female fish to extrude eggs into a clean and dry container; meanwhile, diluting the semen extruded from the male fish with 1-2 times of volume of physiological saline, immediately pouring the diluted semen into the eggs just extruded, immediately and gently stirring the diluted semen for 1-2 min with feathers for artificial insemination, then cleaning the fertilized eggs with clear water, and then incubating.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010168187.1A CN111109166B (en) | 2020-03-11 | 2020-03-11 | Artificial breeding method for acrossocheilus fasciatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010168187.1A CN111109166B (en) | 2020-03-11 | 2020-03-11 | Artificial breeding method for acrossocheilus fasciatus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111109166A CN111109166A (en) | 2020-05-08 |
| CN111109166B true CN111109166B (en) | 2021-12-21 |
Family
ID=70493777
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010168187.1A Active CN111109166B (en) | 2020-03-11 | 2020-03-11 | Artificial breeding method for acrossocheilus fasciatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111109166B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111972324B (en) * | 2020-09-10 | 2022-02-22 | 四川律贝生物科技有限公司 | Breeding and artificial propagation method of schizothorax prenanti |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104663547A (en) * | 2015-03-11 | 2015-06-03 | 中国水产科学研究院长江水产研究所 | Percocypris scaled artificial propagation method |
| CN106489801A (en) * | 2016-12-12 | 2017-03-15 | 福建省淡水水产研究所 | A kind of half pierces thick lip fish artificial seedling rearing's method |
-
2020
- 2020-03-11 CN CN202010168187.1A patent/CN111109166B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104663547A (en) * | 2015-03-11 | 2015-06-03 | 中国水产科学研究院长江水产研究所 | Percocypris scaled artificial propagation method |
| CN106489801A (en) * | 2016-12-12 | 2017-03-15 | 福建省淡水水产研究所 | A kind of half pierces thick lip fish artificial seedling rearing's method |
Non-Patent Citations (1)
| Title |
|---|
| 半刺厚唇鱼人工繁育技术研究;秦志清等;《福建农业学报》;20170515;第32卷(第5期);第486-490页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111109166A (en) | 2020-05-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109090004B (en) | Block type bionic pond propagation seedling raising equipment and method | |
| CN103283652B (en) | Efficient ecological breeding method of giant freshwater prawns | |
| CN112243894B (en) | Artificial feed feeding method for hybrid mandarin fish | |
| CN100464633C (en) | Erythtoculer ilishaefornis and megajobrama amblycephala crossbreeding method | |
| CN107581106B (en) | A kind of artificial fecundation method of platax orbicularis | |
| CN103444610B (en) | Artificial propagation method for acrossocheilus yunnanensis | |
| CN111956783B (en) | Artificial spawning induction mixture for largehead black weever and artificial breeding method | |
| CN109819913A (en) | A kind of artificial breeding method of Porcupinefiss | |
| CN104170778A (en) | Large-scale common sucker breeding method capable of controlling temperature of circulating water | |
| CN110024722A (en) | A kind of artificial fecundation method of scavenger | |
| CN106212333A (en) | A kind of artificial fecundation method of north palpus loach | |
| CN110612930A (en) | Artificial breeding method for rainbow trout | |
| CN117044662A (en) | Method for breeding Furui carps by terrace structure through winter leisure fields and application | |
| CN111109166B (en) | Artificial breeding method for acrossocheilus fasciatus | |
| CN107155971A (en) | Yi Zhong Gan fishes and black carp quasi aromatic hybrid rice breeding method | |
| CN115251010B (en) | Hybridization breeding method for female salmon and male wide fin | |
| CN107439424A (en) | A kind of production of hybrid seeds of Jin Xinwu crucian carps and cultural method | |
| CN112136728B (en) | Artificial breeding method for first-filial generation parent fish of Gymnocypris duringii in circulating water system | |
| CN111226839B (en) | Method for breeding hypoxia-resistant hybrid pelteobagrus fulvidraco | |
| CN106577376A (en) | Hybrid gurnard fry breeding technology | |
| CN107372225A (en) | The artificial breeding method of jaw fish is shoveled in Taiwan | |
| CN113331087A (en) | Artificial propagation method of Gymnocypris ventricosa | |
| CN208987556U (en) | A kind of block type Ecology pond breeding and seedling equipment | |
| CN112352717A (en) | River crab temporary breeding method in winter | |
| CN112219758A (en) | Method for controlling sexual maturity of siganus oramin |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |