CN102017921A - Siniperca chuatsi and siniperca kneri hybridization and culture method - Google Patents
Siniperca chuatsi and siniperca kneri hybridization and culture method Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention relates to the technical field of aquaculture and aquatic breeding, in particular to a siniperca chuatsi and siniperca kneri hybridization and culture method which can be applied to home and collective siniperca culture, especially large-scale culture and breeding. The hybridization and culture method includes the steps of artificial insemination, fry rearing, fostering in pool, culture in pond and the like in details as follows: selecting siniperca chuatsi as female parent fish and siniperca kneri as male parent fish, and strengthening fostering, hatching fries by adopting artificial propagation technical measures of exogenous hormone injection for expediting delivery, running water stimulation for promoting delivery, artificial dry insemination and circular road running water hatching, and then breeding and culturing. The invention provides a novel hybrid siniperca kneri production method, and hybrid siniperca kneri produced by the method has a different shape from that of the female parent, has obvious growth advantage compared with the male parent and keeps the characteristics of fine and smooth meat quality and delicious taste of siniperca kneri.
Description
Affiliated technical field
The present invention relates to fish culture propagation technique field, belong to aquaculture propagation technique field, relate in particular to and stick up mouth mandarin fish and hybridization of big eye mandarin fish and cultural method, can be widely used in family, collective's mandarin fish breed, particularly large-scale cultivation is bred.
Background technology
Stick up mouth mandarin fish (Siniperca chuatsi) and a big eye mandarin fish (Siniperca kneri) is all Perciformes ， Sushi section, mandarin fish subfamily, mandarin fish belongs to fish, 7 ℃-32 ℃ of life thermophilics, 18 ℃-25 ℃ of optimum temperatures, from opening food is food with the live fish and shrimp throughout one's life, is the predacious fish of dwelling at the bottom of the fresh water in the Perciformes.Stick up the mouth mandarin fish and to the east of the Qinling Mountains is supported in middle part, Yunnan to Sichuan, cross the Yellow River to northeast Heilongjiang river from Vietnam of low latitudes distribution is all arranged, extend to the Korea peninsula eastwards.Big eye mandarin fish is now known a provinces and regions to the east of Guangxi of only being distributed on the south the water system of Huaihe River, Guizhou, the Sichuan, is the southern kind of China.Stick up behind the mouth mandarin fish head and back of the body front humps higher, eye less (head length is 5.4-6.8 a times of eye footpath), the maxilla rear end is stretched and is reached under the trailing edge or more, last lower jaw leading portion canine tooth is obvious, and behind the big eye mandarin fish head and back of the body front humps lower, eye big (head length be the eye footpath 4.4-5.8 doubly), after the maxilla rear end is not stretched and is reached under the eyes-affinity, last lower jaw front end canine tooth a little less than.The used big eye mandarin fish of this method is the wild predacious fish that is grown in reservoir, compares with sticking up the mouth mandarin fish, and this flesh of fish exquisiteness is soft, taste is more delicious, but growth is slow, and is individual less.Stick up the mouth mandarin fish from national seed multiplication farm, fast growth, individuality is bigger.If can cultivate the fish that have different qualities and advantage concurrently, then can satisfy the demand of vast consumption.
For addressing the above problem, people have carried out broad research and experiment, such as
Patentee: China Aquatic Science Research Academy Fresh Water Fishery Research Center, the patent (patent No.: CN 200410044894.0) of the title " cross method of a kind of Oreochromis aureus and mandarin fish " of application on May 25th, 2004, open (bulletin) day: 2005.02.23, open (bulletin) number: CN1582650), this invention relates to the cross method of a kind of Oreochromis aureus and mandarin fish, belongs to technical field of living necessaries of humans.It mainly adopts purebred Oreochromis aureus maternal as hybridization, and the hybridization male parent is the male mandarin fish that has the milky seminal fluid to flow out; Adopt ocyodinic to carry out the manual injection, female, the male individuality of Oreochromis aureus all adopts a shot, and male mandarin fish injection ocyodinic also can not injected ocyodinic; , milter female Oreochromis aureus separately raised, and when Tilapia mossambica is discharged ovum, clamp-ons the mandarin fish seminal fluid rapidly, in hatching process, the ovum suction of death is gone, and the wave and culture ware, behind the hybridization fish membrane, change in the aquarium and raise, move into open-air pond again and continue to cultivate the acquisition hybridization fish.The hybridization fish comparison that this invention cultivates is grown fast more than 10% according to Oreochromis aureus, build is short, thick, is suitable for processing.
Suchlike innovation and creation and fish artificial insemination hybridization and breeding method, correlation technique also has a lot, just do not enumerate one by one, in aquaculture propagation technique field, these technical schemes success beyond doubt are feasible, but species variation obviously exists, according to the hybrid vigour principle, to stick up mouth mandarin fish and the hybridization of big eye mandarin fish, by genetic reorganization, through directive breeding, be expected to obtain to possess the hybrid new breed of parent both sides' merit, can obtain fast growth, meat is good, the new varieties that disease-resistant proterties makes moderate progress.Report and yet there are no the technology of sticking up mouth mandarin fish and big eye mandarin fish crossbreeding aspect now.
Summary of the invention
The objective of the invention is to these defectives at the existing mandarin fish of eye greatly, the production method of the big eye of a kind of novel hybridization mandarin fish is provided, the big eye of the hybridization that this method produces mandarin fish is different on form with female parent, compares with male parent to have the obvious growth advantage; Kept simultaneously that big eye mandarin fish meat exquisiteness is soft, the characteristics of delicious flavour.
For developing high-grade fresh water aquaculture, the cultivation mandarin fish is cultured good new varieties a kind of brand-new cost-effective method is provided, and can improve cultured output and quality.
The present invention is achieved in that a kind of mouth mandarin fish and hybridization of big eye mandarin fish and cultural method of sticking up, comprise the steps: (1) artificial insemination: select and stick up that the mouth mandarin fish does maternal parent population, a big eye mandarin fish does the male parent parent population and carries out reinforced cultivating, artificial propagation techniques measures such as exogenous hormone is hastened parturition, stimulation by running water is promoted production by injecting, artificial dry method insemination, the hatching of circuit flowing water hatch fry;
(2) fry rearing: above-mentioned hybridization mandarin fish seedling of hatching two days, the dace of the firm membrane of throwing something and feeding, megalobrama amblycephala fry opening, the grass carp of 3 days firm membranes of throwing something and feeding, silver carp flathead, dace fry behind the opening;
(3) cultivate in the pond: forward in the washing basin about above-mentioned hybridization mandarin fish seedling, growth body length to 1 centimetre and cultivate, carry out sterilization, oxygenation, bait throwing in management;
(4) pond culture: above-mentioned hybridization mandarin fish seedling, about cultivating growth body length to 3 centimetre, the pond forwards pond culture to, before the hybridization mandarin fish is put into the pond, the bait fry of cultivating about 2 centimetres in the pool is as the bait of hybridizing big eye mandarin fish, in time replenish according to the quantity of forage fiss in the pool and the quantity of hybridization mandarin fish later on, carry out sterilization, oxygenation management work simultaneously, after cultivating half a year, therefrom select the big eye of the hybridization mandarin fish that obtains to have the improvement growth traits.
The ocyodinic that described artificial insemination stage injection exogenous hormone is hastened parturition: adopt carp hypophysis (PG), fish to generate releasing hormone analog (LHRH-A with human chorionic gonadtropin (HCG) and short corpus luteum
2) as oxytocic drug, injected dose is PG 3-6 milligram+HCG 800-1200IU+LHRH-A
25-15 microgram/kilogram (raun body weight), injection at twice last time was 1/10th inferior dosage of back, and the milter consumption reduces by half, and only injection is once.Perhaps adopt maleic acid DOM (DOM), LHRH-A
2With HCG as oxytocic drug, injected dose is DOM 3-8 milligram+LHRH-A
25-15 microgram+HCG 1000-1500IU/ kilogram (raun body weight), a shot, the milter consumption reduces by half.
Described artificial insemination stage circuit flowing water hatching, the circuit flow velocity is controlled at about 0.2 meter per second, and water quality meets the regulation of water quality standard for fishery GB11607, PH 6.5-8.5 wherein, dissolved oxygen remains on more than 6 mg/litre, and biochemical oxygen demand (BOD) is bred 23 ℃-29 ℃ of water temperatures less than 4.
Female-male proportion is 2-3 during described artificial insemination: 1, and institute's extracting extract mixes with fish-egg, adds 0.85% salt solution then and stirs 1-2 minute, makes it fertilization.
During described fry rearing, the bait fish length generally is controlled at the 30%-90% of hybridization mandarin fish length, guarantees that the forage fiss height is controlled at the 25%-100% of hybridization mandarin fish bore, and feeding volume is according to the situation decision of ingesting of hybridization mandarin fish.
When cultivated in described pond, hybridization mandarin fish seedling specification reached about 2.0 centimetres, should in time size specification be divided and support, in order to avoid from killing and devouring mutually.
Of the present inventionly a kind ofly stick up the mouth mandarin fish and have following beneficial effect with hybridization of big eye mandarin fish and cultural method:
1. the big eye of the hybridization mandarin fish of the inventive method generation is with maternal different on form, compare with male parent and to have the obvious growth advantage, kept simultaneously that big eye mandarin fish meat exquisiteness is soft, the characteristics of delicious flavour, cultivated growth rate far faster than common big eye mandarin fish (2.25 times), the big eye of individual improvement mandarin fish new varieties greater than common big eye mandarin fish (1.34 times);
2. the present device requirement is low, and operation being easy to realize, can be used for general fish reproduction place; for developing high-grade fresh water aquaculture; the cultivation mandarin fish is cultured good new varieties a kind of brand-new cost-effective method is provided, and can improve cultured output and quality, and suitable large-scale cultivation is bred operation.
Summary of the invention
Specific embodiment
The invention will be further described below by case study on implementation in 2009.
1) parent population and parent fish rearing
1. parent population is chosen: introduce female parent from national mandarin fish seed farm in October, 2008 and stick up mouth mandarin fish parent population, the big eye of male parent is selected from natural waters by mandarin fish according to qualifications; External form should meet the morphological feature of sticking up mouth mandarin fish and big eye mandarin fish, physique stalwartness, anosis, nothing wound; Big eye mandarin fish milter is more than 2 ages, and body weight is more than 0.75 kilogram; Stick up mouth mandarin fish raun more than 3 ages, body weight is more than 1 kilogram.
2. parent fish rearing: the parent population that sticks up mouth mandarin fish and big eye mandarin fish is placed on the pond cultivation of 1-3 mu, depth of water 1.5-1.8 rice respectively.Water quality meets the regulation of water quality standard for fishery GB11607, and wherein dissolved oxygen remains on more than 5 mg/litre.Specially cultivate in the pond.Put in a suitable place to breed about 60 kilograms for every mu.Female milter was with collocation in 1: 1.The forage fiss that sticks up mouth mandarin fish and big eye mandarin fish also can be other similar juvenile fish based on one or more of fish such as elongated dace, wild dace, wheat dace, silver carp, flathead, crucian carp, carp, and specification is less than the bore of parent population.The bait throwing in of parent population drops into forage fiss when putting parent population in a suitable place to breed.Daily ration, feeding quantity is the twice of parent population weight, makes regular check in the pool forage fiss later on and deposits pool situation, look forage fiss storage what, adjust daily ration, feeding quantity, keep that the ratio of forage fiss and mandarin fish parent population weight is about 1: 1.2 in the pond.
3. cultivating and managing: adhere to early, patrol the pool evening, notes observing change of water quality and parent population active situation, carries out the raising daily record; Find fish disease, in time treatment; Every the new water of 15-20 Tianchong once; Laid eggs previous month, and should keep water quality pure and fresh every bath in 7 days.
2) artificial propagation
1. determine the breeding time: Delta of the Pearl River area mandarin fish mating season is 4-6 month, and the breeding water temperature is advisable with 23 ℃-29 ℃.Mating season gonad maturity stick up mouth mandarin fish raun belly expand, soft, the ovary profile is obvious, gonopore lax, the hand belly is soft and high resilience, ventrimeson are recessed, mobile shape, anus redness are arranged after the ovary tenesmus.Big eye mandarin fish male mating season of belly expands, the middle indent of anus is blush, gently press belly to have the milky seminal fluid to flow out, and entry is promptly loose.
2. began artificial propagation on May 6th, 2009, injection ocyodinic: adopt PG, HCG and LHRH-A
2As oxytocic drug, injected dose is PG 3-6 milligram+HCG 800-1200IU+LHRH-A
25-15 microgram/kilogram (raun body weight), injection at twice last time was 1/10th inferior dosage of back, and the milter consumption reduces by half, and only injection is once.Perhaps adopt DOM, LHRH-A
2With HCG as oxytocic drug, injected dose is DOM 3-8 milligram+LHRH-A
25-15 microgram+HCG 1000-1500IU/ kilogram (raun body weight), a shot, the milter consumption reduces by half.
The parent population situation of hastening parturition
3. artificial insemination: female-male proportion is 2-3 when adopting artificial insemination: 1.According to water temperature height and parent population maturity of fish gonads, checked once in 30 minutes at every interval in the time in effect.When light pressure fish belly portion, when gonopore has the ovum grain to flow out, promptly desirable ovum, get essence.Institute's extracting extract is mixed with fish-egg, add 0.85% salt solution then and stirred 1-2 minute, make it fertilization.The apparatus that holds essence, ovum should be dry, clean, and smart, ovum is avoided the sunlight direct projection.
4. hatching: the hatching water adopts storehouse water, and water quality meets the regulation of water quality standard for fishery GB11607, and wherein dissolved oxygen remains on more than 6 mg/litre.The water that enters hatching keeps water quality pure and fresh by the close net filtration of 60 purposes, is strictly on guard against that harmful animal enters.With the circuit hatching, every cube of water body is gone into ten thousand of fertilized egg 10-15.Before circuit is gone into ovum, check filtering window, duckbilled,, regulate current with the potassium permanganate sterilization.For preventing water mold, splash 1-2 time with salt every day in the hatching process, and making the liquor strength of hatching water is 0.3%, and cuts off the water three minutes.Must there be the special messenger on duty between the incubation period, the intact situation of observation and inspection incubation equipment, the floating situation of water quality, streamflow regime, fish-egg; In time scrub filtering window, keep drainage unimpeded, and perform record on duty, pinpointing the problems in time solves.
The hatching situation
5. bait supply: the hybridization mandarin fish seedling behind the membrane adopts fine bourn to cultivate in incubation equipment.Move about when fry is in level, the agreeable to the taste forage fiss of the firm membrane of should in time throwing something and feeding during initial feeding is as megalobrama amblycephala, carp or dace seedling etc., generally two days openings behind membrane.Second and third sky after mandarin fish lays eggs, the megalobrama amblycephala of a collection of capacity of respectively hastening parturition or dace are with fish nest collection ovum.When hybridization mandarin fish seedling reaches two ages in days, there are the fish nest of above-mentioned two batches of megalobrama amblycephalas of hastening parturition, carp ovum or fertilized egg that will membrane to place the incubation equipment of hatching hybridization mandarin fish ovum to hatch collection, make its membrane synchronous with hybridization mandarin fish seedling initial feeding, for hybridization mandarin fish seedling provides the agreeable to the taste bait of capacity, go out incubation equipment until fry.When hybridization mandarin fish seedling reaches 8 ages in days (about 1 centimetre of total length), shift out incubation equipment and change cement pit over to and carry out fry rearing.
3) fry rearing
1. fry rearing mode: 20-40 square metre of small-sized cement pool area, depth of water 0.8-1 rice.Sterilize in advance in the pond of growing seedlings.The water of growing seedlings storehouse water, water quality meets the regulation of water quality standard for fishery GB11607, and wherein dissolved oxygen remains on more than 6.0 mg/litre, and biochemical oxygen demand (BOD) is less than 4, and water temperature is 24 ℃-30 ℃.Little cement pit cultivation is put the 2000-3000 tail for every square metre;
2. bait feeding and supply: the forage fiss specification of throwing something and feeding is controlled at the 30%-90% of hybridization mandarin fish total length, guarantee that the forage fiss height is controlled at the 25%-100% of hybridization mandarin fish bore, throw something and feed and want in good time, an amount of, throw something and feed 1-2 every day nurturing period, and each feeding volume is 3-4 times of hybridization mandarin fish seedling quantity.In time adjust according to forage fiss volume residual in the fishpond when throwing something and feeding.In a planned way be arranged in and cultivate forage fiss in the pond, economy, agreeable to the taste forage fiss supply are arranged to guarantee the later stage.
3. cultivating and managing: the raising through 8-12 days, hybridization mandarin fish seedling specification reaches about 2.0 centimetres, should in time size specification be divided and support, in order to avoid from killing and devouring mutually.Between nursery stage, there is the special messenger to be responsible for daily management, timely adjustment current and inflated size, blowdown in time, cleaning maintain sufficient dissolved oxygen and good water quality condition.Survey sample the fish body every day, the discovery disease is in time treated.Through cultivating big eye mandarin fish cross hybrid seedling length about this 14 days stages to about the 3cm, forward the pond to and culture this moment.
4) pond culture (specially cultivate in the pond)
1. type of rearing: cultivate in special pond, pond, pond area 2.5-3 mu, and 1.5 meters-1.8 meters of the depth of waters, row irrigation is convenient, the strictness sterilization of cleaning up the pond before use in the fishpond.Breeding density should be looked the state of supply and the raise competence of pond condition, forage fiss and determines, puts 4000 tails in a suitable place to breed for general every mu.
2. bait is equipped with and throws something and feeds: after big eye mandarin fish hybridization fish is put in a suitable place to breed, sampling is regularly measured its growth rate, survival rate and is deposited pool amount, on this basis, and simultaneously with reference to factors such as temperature Change, 5%-10% by the big eye of pond hybridization mandarin fish gross weight is a daily ration, feeding quantity, calculates the quantity of throwing in forage fiss.Also can throw something and feed according to the density of checking residual bait fish in the hybridization mandarin fish pond.Throwing something and feeding of forage fiss to throw to well in 5 days one.Cultivate the required a large amount of forage fiss of hybridization mandarin fish for solving, take following two approach: 1. before putting hybridization mandarin fish seedling 10-15 days, earlier put into fries such as triangular bream, silver carp, dace, grass one or more are main in batches, also can be other similar juvenile fish, every mu of density is 20-35 ten thousand tails, send out the pool and every day with rich water and sprinkle soya-bean milk, when forage fiss grows to 2 centimetres of left and right sides, just in time be the agreeable to the taste bait the during pool under the hybridization mandarin fish summer flower.2. with 1: 1-2 prepares forage fiss and cultivates the pond, and one or more are main to put easily numerous, easy breeding classes such as silver carp, triangular bream, dace, wheat dace in a suitable place to breed, also can be other similar juvenile fish, put 50,000 tail summer flowers in a suitable place to breed for every mu and raise together with.To draw in the net by stages, repeatedly be principle on a small quantity then, agreeable to the taste specification fingerling thrown something and fed hybridize mandarin fish.
3. cultivating and managing: every day the early, middle and late pond of patrolling, situation such as observe fish movement, ingest is regularly measured water temperature, dissolved oxygen, pH value, performs record.Carry out water quality management, the new water of in good time annotating keeps water quality pure and fresh, and transparency is about 40 centimetres.Regular (every 30 days) lime water of splashing, concentration is 20 mg/litre, to regulate the pH value.In time open aerator according to weather, water temperature, fish condition.Guarantee water body dissolved oxygen abundance (24 hours more than 5 mg/litre).Rely mainly on prevention, carry out disease control work, on the basis of the clear pond of strictness sterilization, the breeding water body of regularly sterilizing externally advances forage fiss and will adhere to quarantine, sterilization, prevents that pathogene from bringing hybridization mandarin fish breeding water body into.
5) artificial selection
In April, 2010 in pond culture 11 months big eye mandarin fish hybridization fish carry out artificial selection, obtain the big eye of hybridization mandarin fish 678 tails when cleaning up the pond, select individuality 100 tails that wherein body length is bigger to measure the long scope of body at 27.24 ± 18.8cm, weight range is at 456.3 ± 120.2g, compare, a wild age big eye mandarin fish also just has the body weight of 205 ± 88.27g, 20.41 ± 2.57cm (sample 276 tails) in the reservoir.The weight average growth rate of the big eye of hybridization mandarin fish is 2.25 times of wild big eye mandarin fish, and average body length is 1.34 times of wild big eye mandarin fish.Therefore the big eye of a hybridization mandarin fish individuality has obvious growth proterties advantage, selects that build is big, growth rate obviously continues to culture faster than the big eye of the hybridization mandarin fish of common big eye mandarin fish.
Certainly, the invention is not restricted to the foregoing description, modification and equivalent transformation that the spirit of Fan Yiben creation is done, or adopt various deformation on this basis, all belong in the protection domain of the present invention.
Claims (6)
1. one kind sticks up mouth mandarin fish and hybridization of big eye mandarin fish and cultural method, and its feature comprises the steps:
(1) artificial insemination: select and stick up that the mouth mandarin fish does maternal parent population, a big eye mandarin fish does the male parent parent population and carries out reinforced cultivating, by the injection exogenous hormone hasten parturition, artificial propagation techniques measures such as stimulation by running water is promoted production, artificial dry method insemination, the hatching of circuit flowing water, hatch fry;
(2) fry rearing: above-mentioned hybridization mandarin fish seedling of hatching two days, the dace of the firm membrane of throwing something and feeding, megalobrama amblycephala fry opening, the grass carp of 3 days firm membranes of throwing something and feeding, silver carp flathead, dace fry behind the opening;
(3) cultivate in the pond: forward in the washing basin about above-mentioned hybridization mandarin fish seedling, growth body length to 1 centimetre and cultivate, carry out sterilization, oxygenation, bait throwing in management;
(4) pond culture: above-mentioned hybridization mandarin fish seedling, about cultivating growth body length to 3 centimetre, the pond forwards pond culture to, before the hybridization mandarin fish is put into the pond, the bait fry of cultivating about 2 centimetres in the pool is as the bait of hybridizing big eye mandarin fish, in time replenish according to the quantity of forage fiss in the pool and the quantity of hybridization mandarin fish later on, carry out sterilization, oxygenation management work simultaneously, after cultivating half a year, therefrom select the hybridization mandarin fish that obtains to have the improvement growth traits.
2. mouth mandarin fish and hybridization of big eye mandarin fish and the cultural method of sticking up according to claim 1 is characterized in that: the ocyodinic that described artificial insemination stage injection exogenous hormone is hastened parturition: adopt carp hypophysis (PG), fish to generate releasing hormone analog (LHRH-A with human chorionic gonadtropin (HCG) and short corpus luteum
2) as oxytocic drug, injected dose is PG 3-6 milligram+HCG 800-1200IU+ LHRH-A
25-15 microgram/kilogram (raun body weight), injection at twice last time was 1/10th inferior dosage of back, and the milter consumption reduces by half, and only injection is once.Perhaps adopt maleic acid DOM (DOM), LHRH-A
2With HCG as oxytocic drug, injected dose is DOM 3-8 milligram+LHRH-A
25-15 microgram+HCG 1000-1500IU/ kilogram (raun body weight), a shot, the milter consumption reduces by half.
3. mouth mandarin fish and hybridization of big eye mandarin fish and the cultural method of sticking up according to claim 1, it is characterized in that: described artificial insemination stage circuit flowing water hatching, the circuit flow velocity is controlled at about 0.2 meter per second, water quality meets the regulation of water quality standard for fishery GB11607, PH 6.5-8.5 wherein, dissolved oxygen remains on more than 6 mg/litre, and biochemical oxygen demand (BOD) is bred 23 ℃-29 ℃ of water temperatures less than 4.
4. mouth mandarin fish and hybridization of big eye mandarin fish and the cultural method of sticking up according to claim 1 is characterized in that: female-male proportion is 2-3 during described artificial insemination: 1, and institute's extracting extract mixes with fish-egg, adds 0.85% salt solution stirring then 1-2 minute, makes it to be fertilized.
5. mouth mandarin fish and hybridization of big eye mandarin fish and the cultural method of sticking up according to claim 1, it is characterized in that: during described fry rearing, the bait fish length generally is controlled at the 30%-90% of hybridization mandarin fish length, guarantee that the forage fiss height is controlled at the 25%-100% of hybridization mandarin fish bore, feeding volume determines according to the hybridization mandarin fish situation of ingesting.
6. mouth mandarin fish and hybridization of big eye mandarin fish and the cultural method of sticking up according to claim 1 is characterized in that: when cultivated in described pond, hybridization mandarin fish seedling specification reached about 2.0 centimetres, should in time size specification be divided and support, in order to avoid from killing and devouring mutually.
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| CN110419470A (en) * | 2019-08-05 | 2019-11-08 | 华中农业大学 | A kind of indoor industrially propagation method of mandarin fish |
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