CN107114281A - A kind of Culter Yu Yu Gan fish quasi aromatic hybrid rice breeding methods - Google Patents

A kind of Culter Yu Yu Gan fish quasi aromatic hybrid rice breeding methods Download PDF

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Publication number
CN107114281A
CN107114281A CN201710475263.1A CN201710475263A CN107114281A CN 107114281 A CN107114281 A CN 107114281A CN 201710475263 A CN201710475263 A CN 201710475263A CN 107114281 A CN107114281 A CN 107114281A
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China
Prior art keywords
culter
parent
fish
gan
fishes
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CN201710475263.1A
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Chinese (zh)
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杨品红
周波兰
杨春英
夏虎
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Hunan University of Arts and Science
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Hunan University of Arts and Science
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Priority to CN201710475263.1A priority Critical patent/CN107114281A/en
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00—Culture of aquatic animals
    • A01K61/10—Culture of aquatic animals of fish
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/22—Animal feeding-stuffs from material of animal origin from fish
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/174—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y02A40/818—Alternative feeds for fish, e.g. in aquacultures

Abstract

The invention discloses a kind of Culter Yu Yu Gan fish quasi aromatic hybrid rice breeding methods, by the selection of parent, the reinforced cultivating of parent, hasten parturition, artificial insemination, hatch these steps and realize.Li Yong Gan fishes of the present invention hybridize with Culter fishes, the merit of Zong He Gan fishes and Culter fishes, through culture experiment, hybridization F1The features such as generation shows individual big, fast growth, lower oxygen concentration resistance, good meat strong to the resistivity of disease and fresh mouthfeel, with important application value in production, has promoted the sound development of China's culture fishery.

Description

A kind of Culter Yu Yu Gan fish quasi aromatic hybrid rice breeding methods
Technical field
The present invention relates to fish new lines breeding technical field, and in particular to a kind of Culter Yu Yu Gan fish quasi aromatic hybrid rices breeding side Method.
Background technology
Culter fishes, belong to Cypriniformes, Cyprinidae, including stick up mouth Culter, Mongolia Culter, tip Culter etc., be China four big fresh water name fish it Head, Culter fish Individual Size great disparity, the maximum mouth Culter that sticks up can grow to more than 10 kilograms, and Mongolia Culter only has about 1 kilogram, and some Being referred to as of side " green grass or young crops ties up " up to formula Culter then less than 500 grams, disease is few, can lower oxygen concentration resistance, parent population lays eggs in the 5-7 months.Culter fish nutrition It is abundant, it is in good taste, but fishbone is more.
Gan fishes, belong to Cypriniformes, and Cyprinidae , Gan category, body is elongated, sub- cylindrical shape, kiss point, in beak.Body carries on the back taupe, belly Silvery white, dorsal fin, tail fin Dark grey are a kind of large-scale food meat fish, and natural disposition is violent, and fast growth, individual is big, and meat is fresh U.S., disease resistance is weak, not lower oxygen concentration resistance.Sexal maturity is 3-4 ages, and parent population lays eggs in the 4-6 months in rivers torrent.
Therefore be necessary that Culter Yu, Gan fishes are studied and tested, to obtain that fast growth, meat are good, disease resistance is strong, The new varieties of energy lower oxygen concentration resistance.By literature search, do not find and the same or analogous open report of the present invention.
The content of the invention
The deficiency existed for above-mentioned prior art, it is an object of the invention to provide a kind of Culter Yu Yu Gan fish quasi aromatic hybrid rices Breeding method.
To achieve the above object, the technical solution adopted by the present invention：A kind of Culter Yu Yu Gan fish quasi aromatic hybrid rice breeding methods, By the selection of parent, the reinforced cultivating of parent, hasten parturition, artificial insemination, hatch these steps and realize, it is characterised in that
The reinforced cultivating concrete operations of above-mentioned parent are：The Culter fish female parents that will be singled out are put into Culter fish parent cultures pond, Start to feed including barrenwort 8-15%, cornu bubali 5-10%, motherwort 10-20%, B group vitamin 2- within 2-3 months before hybridization 5%th, the bait including coconut juice 5-10%, zymosan 3-5%, Semen Cuscutae powder 5-10%, eel bone meal 10-30%, soft-shelled turtle egg 5-10%, with Percent by weight, day throwing amount is the tails of 1000-2000kg/ ten thousand, and controls culture pond water temperature to be 22-25 DEG C, promotes sexual gland Development；It will be singled out in Gan fish male parent's Fang Ru Gan fish parent cultures pond, feed including fresh fish within 2-3 months before hybridization Block 85-95%, B B-complex 5-15% bait, day throwing amount are the tails of 500-1000kg/ ten thousand, and reinforced cultivating is carried out to parent；
The concrete operations of above-mentioned artificial insemination are：Parent after conscientiously observation is hastened parturition, when both of which full maturity, adopts Culter fishes Mature egg is Ji the mature sperm of Gan fishes, essence:The volume ratio of ovum is 1:3-5 is sufficiently mixed, i.e., the quantity ratio of smart ovum is more than 5 Hundred million：1, temperature control is at 22-24 DEG C, and time of insemination 3-5min is stand-by；
The concrete operations of above-mentioned hatching are：The embryonated egg after artificial insemination is taken, is put into circular hatching channal and carries out Lotic hatching, is hatched With the control of water pH value in 8.0-8.4, dissolved oxygen is 6.5-8.0mg/L, and water temperature control is 25-27 DEG C, and water velocity is 3-5m/min； When hatching is arrived 2-3 hours, embryonated egg is put into 0-1 DEG C, 3-4min in the cold water added with herbal mediciment extract, in cold water Dissolved oxygen is maintained at more than 5.0mg/L；Then take out and hatched once again in the water body of incubator, until emerging grows up to spray.
Preferably, in above-mentioned incubation step herbal mediciment extract is mainly prepared from by the raw material of following parts by weight： 20-30 parts of taxol, 10-15 parts of nightshade total alkali, 8-10 parts of angelica extract, 8-10 parts of Astragalus Root P.E.
Compared with prior art, the beneficial effects of the invention are as follows：Li Yong Gan fishes of the present invention and Culter fishes hybridize, Zong He Gan fishes and The merit of Culter fishes, through culture experiment, hybridization F1In generation, shows individual big, fast growth, lower oxygen concentration resistance, to disease Resistivity is strong, meat is good and the features such as fresh mouthfeel, has important application value in production, has promoted China's aquaculture The sound development of industry.
Embodiment
In conjunction with specific embodiment, next the invention will be further elaborated.
Embodiment one
Mouth Culter Yu Gan fish quasi aromatic hybrid rice breeding methods are stuck up, are comprised the following steps：
（1）The selection of parent：The Mongolian red Culter females for choosing 4-5 ages, body weight 500-5000g, gonadal maturation and health are made For female parent；Choose 3 more than age, body weight 7-20kg, gonadal maturation and Jian Kang Gan fishes male as male parent, 12 before hybridization Individual month, just allow female, male in parent independent cultivate；
（2）The reinforced cultivating of parent：By step（1）The Mongolian Culter fishes female parent picked out is put into Culter fish parent cultures pond, Start within 2 months before hybridization to feed by barrenwort 15%, cornu bubali 10%, motherwort 10%, B groups vitamin 5%, coconut juice 10%, yeast The bait that polysaccharide 3%, Semen Cuscutae powder 5-%, eel bone meal 30%, soft-shelled turtle egg 5% are formulated, day throwing amount is the tails of 1000kg/ ten thousand, and is controlled Culture pond water temperature processed is 25 DEG C, promotes gonad development；It will be singled out Gan fish male parent's Fang Ru Gan fish parent cultures pond In, the bait being made up of fresh fish block 95%, B B-complex 5% is fed within 2 months before hybridization, day throwing amount is the tails of 500kg/ ten thousand, Reinforced cultivating is carried out to parent；
（3）Hasten parturition：To passing through step（2）The maternal Mongolia Culter fishes injection ocyodinic LRH-A 0.25ug+HCG100 IU/ of processing Kg fish body weights, Luteinizing hormone releasing hormone A2 60ug/ are promoted to the injection of this Gan of Fu fishes by carp pituitary 0.9mg/kg fish bodies weight, injection The ocyodinic of kg fish bodies weight, the IU/kg fish bodies of injection chorionic gonadotrophin 3 weight；
（4）Artificial insemination：Conscientious observation of steps（3）Parent, when both of which full maturity, adopt the mature egg of Culter fishes Ji Gan The mature sperm of fish, essence:The volume ratio of ovum is 1:3 are sufficiently mixed, i.e., the quantity ratio of smart ovum is more than 500,000,000：1, temperature control At 24 DEG C, time of insemination 5min is stand-by；
（5）Hatching：Take step（4）In embryonated egg, be put into circular hatching channal and carry out Lotic hatching, Spawning water pH value control exist 8.4, dissolved oxygen is 8.0mg/L, and water temperature control is 27 DEG C, and water velocity is 5m/min；When hatching was by 2 hours, embryonated egg is put Enter 0 DEG C, 3min in the cold water added with herbal mediciment extract, herbal mediciment extract prepared by the raw material of following parts by weight and Into：30 parts of taxol, 10 parts of nightshade total alkali, 8 parts of angelica extract, 10 parts of Astragalus Root P.E；Cold dissolved oxygen in water is maintained at More than 5.0mg/L, suppresses its chromosome separation, it is smoothly doubled fusion, forms tetraploid；Then take out once again in incubator Water body in hatched, until emerging and growing up to spray.
Embodiment two
Mouth Culter Yu Gan fish quasi aromatic hybrid rice breeding methods are stuck up, are comprised the following steps：
（1）The selection of parent：The erythroculter ilishaeformis female for choosing 4-5 ages, body weight 500-5000g, gonadal maturation and health is made For female parent；Choose 3 more than age, body weight 7-20kg, gonadal maturation and Jian Kang Gan fishes male as male parent, 6 before hybridization Individual month, just allow female, male in parent independent cultivate；
（2）The reinforced cultivating of parent：By step（1）The mouth Culter fish female parents of sticking up picked out are put into Culter fish parent cultures pond, Start within 3 months before hybridization to feed many by barrenwort 8%, cornu bubali 5%, motherwort 20%, B groups vitamin 2%, coconut juice 5%, yeast The bait that sugar 5%, Semen Cuscutae powder 10%, eel bone meal 10%, soft-shelled turtle egg 10% are prepared, day throwing amount is the tails of 2000kg/ ten thousand, and controls to cultivate Water temperature is 22 DEG C, promotes gonad development；It will be singled out in Gan fish male parent's Fang Ru Gan fish parent cultures pond, miscellaneous The bait for feeding for first 3 months and being made up of fresh fish block 85%, B B-complex 15% is handed over, day throwing amount is the tails of 1000kg/ ten thousand, to parent Carry out reinforced cultivating；
（3）Hasten parturition：To passing through step（2）The female parent of processing sticks up mouth Culter fishes injection ocyodinic LRH-A 0.25ug+HCG100 IU/ Kg fish body weights, Luteinizing hormone releasing hormone A2 60ug/ are promoted to the injection of this Gan of Fu fishes by carp pituitary 0.6mg/kg fish bodies weight, injection The ocyodinic of kg fish bodies weight, the IU/kg fish bodies of injection chorionic gonadotrophin 1.5 weight；
（4）Artificial insemination：Conscientious observation of steps（3）Parent, when both of which full maturity, adopt the mature egg of Culter fishes Ji Gan The mature sperm of fish, essence:The volume ratio of ovum is 1:5 are sufficiently mixed, i.e., the quantity ratio of smart ovum is more than 500,000,000：1, temperature control At 22 DEG C, time of insemination 5min is stand-by；
（5）Hatching：Take step（4）In embryonated egg, be put into circular hatching channal and carry out Lotic hatching, Spawning water pH value control exist 8.0, dissolved oxygen is 6.5mg/L, and water temperature control is 25 DEG C, and water velocity is 3m/min；When hatching was by 3 hours, embryonated egg is put Enter 1 DEG C, 4min in the cold water added with herbal mediciment extract, herbal mediciment extract prepared by the raw material of following parts by weight and Into：20 parts of taxol, 15 parts of nightshade total alkali, 10 parts of angelica extract, 8 parts of Astragalus Root P.E；Cold dissolved oxygen in water is maintained at More than 5.0mg/L, suppresses its chromosome separation, it is smoothly doubled fusion, forms tetraploid；Then take out once again in incubator Water body in hatched, until emerging and growing up to spray.
Inventor's the method according to the invention is obtained to continue to track after spray and fed to 3 ages, and 1 is randomly selected during feeding Age, 2 ages, each 20 tail of hybridization fish in 3 ages, and randomly choose the correspondence age of a fish stick up mouth Culter, Gan fish, every kind of each tail of the age of a fish 20, point Ce Ding not body length, live body the quantitative character parameter such as again.Specific data refer to following table one.
Table one：
It was found from the above-mentioned data of table one, under cultivating condition, using stick up mouth Culter as maternal , Gan fishes be male parent obtain new product of the present invention Kind, the 1-3 age speeds of growth fall between, and total length ratio sticks up mouth Culter growths fast 15.3%, 38.3%, 16.8%, and weight ratio sticks up mouth Culter Fast 0.66 times, 1.76 times, 0.48 times.

Claims (2)

1. a kind of Culter Yu Yu Gan fish quasi aromatic hybrid rice breeding methods, by the selection of parent, the reinforced cultivating of parent, hasten parturition, people Work is inseminated, hatches these steps to realize, it is characterised in that
The reinforced cultivating concrete operations of above-mentioned parent are：The Culter fish female parents that will be singled out are put into Culter fish parent cultures pond, Start to feed including barrenwort 8-15%, cornu bubali 5-10%, motherwort 10-20%, B group vitamin 2- within 2-3 months before hybridization 5%th, the bait including coconut juice 5-10%, zymosan 3-5%, Semen Cuscutae powder 5-10%, eel bone meal 10-30%, soft-shelled turtle egg 5-10%, with Percent by weight, day throwing amount is the tails of 1000-2000kg/ ten thousand, and controls culture pond water temperature to be 22-25 DEG C, promotes sexual gland Development；It will be singled out in Gan fish male parent's Fang Ru Gan fish parent cultures pond, feed including fresh fish within 2-3 months before hybridization Block 85-95%, B B-complex 5-15% bait, day throwing amount are the tails of 500-1000kg/ ten thousand, and reinforced cultivating is carried out to parent；
The concrete operations of above-mentioned artificial insemination are：Parent after conscientiously observation is hastened parturition, when both of which full maturity, adopts Culter fishes Mature egg is Ji the mature sperm of Gan fishes, essence:The volume ratio of ovum is 1:3-5 is sufficiently mixed, i.e., the quantity ratio of smart ovum is more than 5 Hundred million：1, temperature control is at 22-24 DEG C, and time of insemination 3-5min is stand-by；
The concrete operations of above-mentioned hatching are：The embryonated egg after artificial insemination is taken, is put into circular hatching channal and carries out Lotic hatching, is hatched With the control of water pH value in 8.0-8.4, dissolved oxygen is 6.5-8.0mg/L, and water temperature control is 25-27 DEG C, and water velocity is 3-5m/min； When hatching is arrived 2-3 hours, embryonated egg is put into 0-1 DEG C, 3-4min in the cold water added with herbal mediciment extract, in cold water Dissolved oxygen is maintained at more than 5.0mg/L；Then take out and hatched once again in the water body of incubator, until emerging grows up to spray.
2. Culter Yu Yu Gan fish quasi aromatic hybrid rice breeding methods according to claim 1, it is characterised in that：Above-mentioned incubation step In herbal mediciment extract be mainly prepared from by the raw material of following parts by weight：20-30 parts of taxol, nightshade total alkali 10-15 Part, 8-10 parts of angelica extract, 8-10 parts of Astragalus Root P.E.
CN201710475263.1A 2017-06-21 2017-06-21 A kind of Culter Yu Yu Gan fish quasi aromatic hybrid rice breeding methods Pending CN107114281A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108925472A (en) * 2018-07-02 2018-12-04 华中农业大学 A kind of Bambusa and megalobrama amblycephala cross breeding method

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106172117A (en) * 2016-07-14 2016-12-07 浙江省海洋水产研究所 Carnis Pseudosciaenae raun and Carnis Pseudosciaenae milter cross breeding method

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106172117A (en) * 2016-07-14 2016-12-07 浙江省海洋水产研究所 Carnis Pseudosciaenae raun and Carnis Pseudosciaenae milter cross breeding method

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108925472A (en) * 2018-07-02 2018-12-04 华中农业大学 A kind of Bambusa and megalobrama amblycephala cross breeding method

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