CN115530099A - Acipenser parvum female fish and huso male fish hybrid breeding method - Google Patents

Acipenser parvum female fish and huso male fish hybrid breeding method Download PDF

Info

Publication number
CN115530099A
CN115530099A CN202211327432.4A CN202211327432A CN115530099A CN 115530099 A CN115530099 A CN 115530099A CN 202211327432 A CN202211327432 A CN 202211327432A CN 115530099 A CN115530099 A CN 115530099A
Authority
CN
China
Prior art keywords
fish
female
feeding
artificial
male
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202211327432.4A
Other languages
Chinese (zh)
Inventor
张颖
曹顶臣
孙志鹏
吕伟华
韩世成
刘霞飞
丰超杰
王若愚
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences
Original Assignee
Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences filed Critical Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences
Priority to CN202211327432.4A priority Critical patent/CN115530099A/en
Publication of CN115530099A publication Critical patent/CN115530099A/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00—Culture of aquatic animals
    • A01K61/10—Culture of aquatic animals of fish
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/174—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish

Abstract

A method for breeding young sturgeon female fish and huso dauricus male fish by hybridization belongs to the technical field of aquaculture. The method comprises the following steps: (1) Selecting healthy acipenser parvum female fish and huso dauricus male fish, wherein the ages of the acipenser parvum female fish and the huso dauricus male fish are respectively more than 4 ages and 14 ages; (2) parent fish breeding; (3) feeding parent fishes; strengthening nutrition; reproductive regulation and daily management; step two: artificial propagation; comprises artificial induced spawning; extruding eggs and collecting semen; artificial fertilization; washing eggs and hatching fertilized eggs; and step three, breeding the fish larvae and the juvenile fish. The hybrid sturgeon fries cultivated by the method have the characteristics of high fertility rate and hatchability, fine and smooth meat quality, fast growth, wide adaptability, strong stress resistance and other excellent growth characters, can cultivate new sturgeon species, can realize large-scale cultivation of new species fries, and is suitable for sturgeon cultivation in different areas of China.

Description

Acipenser parvum female fish and huso male fish hybrid breeding method
Technical Field
The invention belongs to the technical field of aquaculture, and particularly relates to a method for hybrid breeding of female acipenser baeri and male huso sturgeon.
Background
At present, china has become the biggest sturgeon breeding country and caviar producing country in the world, the annual output reaches 9 thousand tons, and accounts for more than 80 percent of the sturgeon breeding output in the world. With the rapid development of sturgeon breeding industry in China, the problems of mixed breeding varieties, lack of excellent varieties and the like are increasingly prominent, and the healthy development of sturgeon industry in China is severely restricted. At present, sturgeon breeding varieties in China are mostly wild domesticated species, breeding varieties in production are disordered, breeding varieties are mostly disordered hybrid species in small farms, and breeding performance is low. At present, two certified hybrid sturgeon varieties exist in China, production of caviar is mainly used, new sturgeon varieties mainly selected according to meat quality characters are lacked, and the output of the sturgeon for egg accounts for about 30% of the output of the sturgeon in China. Therefore, the new sturgeon variety suitable for local culture in China is urgently needed to be cultured in China, and the structure of the sturgeon culture variety in China is optimized and improved.
The acipenser parvum is a sturgeon variety introduced earlier in China and has the advantages of early sexual maturity, fine and smooth meat quality, good open domestication effect and the like. Huso beluga belongs to sturgeon huso in sturgeon family, is one of the only huso in the world, has the advantages of large individuals, strong disease resistance, high growth speed and the like, is one of important sturgeon germplasm resources in the world, but has the defects of difficult acceptance of artificial feed, long sexual maturation time and the like, and has little breeding amount in China. In the last 90 s of the last century, hybrid sturgeons BESTER (huso male parent and small male parent) are introduced in China, but the variety has low achievement rate and slow growth in China and gradually quits the sturgeon culture market in China.
Disclosure of Invention
The invention aims to provide a method for cross breeding of female acipenser dauricus and male huso sturgeon, which provides a new high-quality sturgeon breeding variety for the sturgeon industry and solves the problem of few sturgeon good varieties in China.
The technical scheme adopted by the invention is as follows:
a method for breeding seedlings of Acipenser parvum female fishes and huso luo male fishes in a hybrid mode comprises the following steps:
step one, selecting, cultivating and reproductive control of parent fishes;
(1) Selecting parent fishes: selecting healthy acipenser parvum female fish and huso dauricus male fish, wherein the ages of the acipenser parvum female fish and the huso dauricus male fish are respectively more than 4 ages and 14 ages;
(2) Parent fish breeding: placing the selected female sturgeon and huso dauricus male fish into a parent fish culture pond, wherein the number ratio of the female and male parent fish is 3:1, feeding, namely feeding artificially matched sturgeon feed, wherein the feeding times per day are 2 times, and the daily feeding amount is 1.5% -2% of the total weight of male and female parent fishes;
when the gonad grows to the early stage IV, starting to perform nutrition-enhanced cultivation on the parent fish, feeding enhanced cultivation bait for 2 times a day, wherein the feeding amount per day is 1.5-2% of the total weight of the parent fish; feeding 2 times a day when gonad development reaches stage V, wherein the feeding amount is 1.5-2% of the total weight of parent fish; breeding parent fish before spawning and hatching;
(3) And (3) reproductive regulation: carrying out reproduction control on parent fish in the parent fish gonad development process;
step two, artificial propagation;
(1) Artificial hastening parturition: when the gonad grows to the V stage, injecting oxytocic into the female sturgeon and the huso sturgeon in water at the temperature of 16-18 ℃;
(2) Egg extruding and semen collection:
(3) Artificial fertilization:
(4) And (3) debonding: pouring the washed fertilized eggs into a debonding device containing 10% of talcum powder aqueous solution, and debonding for 40-60min;
(5) Hatching fertilized eggs: after the debonded fertilized eggs are washed clean, the fertilized eggs are incubated, the water temperature is controlled to be 17.5-18 ℃, the pH value is 7-9, and after the fertilized eggs are incubated for 6-7 days, the young and the larval fish are hatched out by stripping;
step three, breeding the larvae and the juvenile fish;
culturing the newly-emerged young fishes in a culture pond for 8-10 days without feeding, controlling the water temperature to be 17-20 ℃, controlling the pH to be 7-9 and the dissolved oxygen to be more than or equal to 6mg/L, and culturing by running water or circulating water; after the larvae are opened, feeding tubificidae segments, wherein the feeding amount is 10wt% of the weight of the larvae each time, and feeding 8 times a day; after 7 days of opening, adopting special artificial mixed feed for sturgeon and tubificidae segments to make a feed group, wherein the weight ratio of the artificial mixed feed to the tubificidae segments is 20:80, starting artificial bait domestication, feeding 8 times a day, wherein the feeding amount is 10wt% of the weight of the larvae and the young fishes each time; after 5-7 days of domestication, increasing the weight ratio of the artificial mixed feed, simultaneously reducing the weight ratio of tubificidae sections, domesticating for 4 weeks, and then completely feeding the artificial mixed feed.
Further, in the step one (1), parent fish selection is carried out at the beginning of 5 months every year, female sturgeon with normal shape, normal body color and health and no injury is selected, and the weight reaches 4-6 kg/tail; selecting huso dauricus male fish with normal body color, large size, and health, and weight of 60-80 kg/tail.
Further, in the step one (2), the reinforced cultivation bait is prepared from the following raw materials in parts by weight: 10 parts of tenebrio molitor powder, 84 parts of artificially-mixed sturgeon feed, 0.5 part of vitamin C, 0.2 part of vitamin E, 0.3 part of taurine and 5 parts of vegetable powder.
Further, in the step one (3), the reproduction control of the parent fish is specifically as follows: when the natural water temperature is reduced to be below 4 ℃, maintaining the water temperature of 4 ℃ to 30-60 days, then starting to rise from 4 ℃, slowly rising 1 ℃ every 3 days until the temperature rises to 14 ℃, and then maintaining the water temperature unchanged, regularly observing the gonad development condition of the parent fish, and meeting the cultivation condition and carrying out daily management.
Further, in the step two (1), the oxytocic is LHRH-A 2 And a DOM; the injection dosage of the female sturgeon is as follows: LHRH-A 2 6-10 mug/kg body weight and DOM 4mg/kg body weight, mixing and injecting; the injection dosage of the huso sturgeon oxytocic is reduced by half.
Further, in the second step (2), the specific operation steps of egg extruding and semen collecting are as follows:
fishing out huso dauricus male fish after injecting oxytocic for 10-12 hours, squeezing while collecting semen with a sealing bag, storing at low temperature of 2-4 deg.C for use, performing semen microscopic examination before artificial fertilization, selecting semen with strong sperm activity as qualified semen by microscopic examination, and waiting for artificial fertilization; then fishing the female sturgeon, extruding the eggs by a pressing method, and flowing into an egg containing basin to wait for fertilization.
Further, in the second step (3), the specific operation steps of artificial insemination are as follows:
adopting dry artificial insemination, examining qualified semen by using 15-20ml of microscope for each kilogram of roes, putting the semen into an egg containing basin, uniformly stirring the semen by using feathers, then adding hatching water with the amount 4 times that of the roes, continuously and rapidly stirring the semen for 15s, activating the semen to fully combine the sperm and the roes, standing the fertilized roes for 3-5min, discarding sewage, and rinsing the roes clean.
Further, in the third step, the weight ratio of the artificial compound feed to the tubificidae added in 4 weeks is as follows:
in week 1, the weight ratio of the artificial compound feed to the tubificidae segments is 40:60, adding a solvent to the mixture;
in week 2, the weight ratio of the artificial compound feed to the tubificidae segments is 60:40;
in week 3, the weight ratio of the artificial compound feed to the tubificidae segments is 80:20;
in the 4 th week, the weight ratio of the artificial mixed feed to the tubificidae sections is 100:0;
feeding the fish fries for 8 times every day, wherein the feeding amount of each time is 10wt% of the weight of the fish fries; after domestication for 4 weeks, the fish larvae grow into juvenile fish, and the artificial mixed feed is completely ingested; normally managing the fry in the fry stage, measuring the weight of the fry every 10 days, feeding the fry with feed according to 5wt% of the weight of the fry, and feeding the fry 3 times a day.
Compared with the prior art, the invention has the beneficial effects that: the hybrid sturgeon fingerlings cultivated by the method have high fertilization rate (up to more than 75%) and hatching rate (up to more than 80%), and have the characteristics of fine and smooth meat quality, fast growth, wide adaptability, strong stress resistance and other excellent growth traits. The method can cultivate new sturgeon species, realize large-scale cultivation of new species fingerlings, and is suitable for sturgeon cultivation in different areas of China.
Detailed Description
The first specific implementation way is as follows: the embodiment discloses a method for breeding seedlings by hybridizing female acipenser parvus and male huso dauricus, which comprises the following steps:
step one, selecting, cultivating and reproductive control of parent fishes;
(1) Selecting parent fishes: selecting healthy acipenser parvum female fish and huso dauricus male fish, wherein the ages of the acipenser parvum female fish and the huso dauricus male fish are respectively more than 4 years old and 14 years old;
(2) Parent fish breeding: placing the selected female acipenser parvum and male huso dauricus into a parent fish culture pond (length multiplied by width: 6m multiplied by 3 m), wherein the number ratio of the female and male parent fish is 3:1 (feeding density 25 kg/m) 2 ) Feeding, namely feeding artificially-matched sturgeon feed (the sturgeon feed is a Shandong Shengsuo fish feed produced by Shandong Shengsuo fish feed research center), wherein the feeding times are 2 times (2 times with 12-hour intervals) every day, and the daily feeding amount is 1.5-2% of the total weight of male and female parent fish;
when the gonads develop to the early stage IV (in 4 months), starting to perform nutrition-enhanced cultivation of the parent fishes, feeding enhanced cultivation baits (improving reproductive performance), and feeding for 2 times a day (2 times with 12-hour intervals), wherein the daily feeding amount is 1.5-2% of the total weight of the parent fishes;
feeding 2 times per day (2 times with 12 hr interval) until gonad development reaches stage V, wherein the daily feeding amount is 1.5-2% of the total weight of parent fish; breeding parent fish before spawning and hatching;
(3) And (3) reproductive regulation: carrying out reproduction control on parent fish in the parent fish gonad development process;
step two, artificial propagation;
(4) Artificial hastening parturition: when the gonad grows to the V stage (in the middle of 5 months), injecting oxytocic into the female acipenser baeri and the male huso sturgeon in water of 16-18 ℃;
(5) Egg extruding and semen collection:
(6) Artificial fertilization:
(4) And (3) debonding: pouring the washed fertilized eggs into a debonding device containing 10% of talcum powder aqueous solution, and debonding for 40-60min;
(5) Hatching fertilized eggs: after the debonded fertilized eggs are washed clean, a barrel-type incubator is adopted for incubation, the water temperature is controlled to be 17.5-18 ℃, the pH is 7-9, and after the fertilized eggs are incubated for 6-7 days, the young fishes are hatched out by stripping;
step three, breeding the fish larvae and the juvenile fish;
the young fish just emerged from the membrane is not fed for 8-10 days and cultured at 10m 2 Circular glass fiber reinforced plastic culture pond (culture density of 5000 tails/m) 2 Water depth of 30-40 cm), water temperature of 17-20 deg.C (preferably 18-20 deg.C, or 17-19 deg.C), pH of 7-9, dissolved oxygen amount of more than or equal to 6mg/L, and running water or circulating water culture; after the larvae are opened, feeding water earthworm segments (after chopping live baits), wherein the feeding amount is 10wt% of the weight of the larvae per time, and feeding 8 times per day (feeding 1 time every 3 hours); after 7 days from the opening (the body length of the fish larvae is about 2.2cm, at this time, the cultivation density becomes 2500 tails/m 2 ) The special artificial compound feed for sturgeons (Acipenser sinensis feed, produced by a research center for Shandong Acipenser sinensis feed, and is sturgeon microparticle initial feed) and the tubificidae section are adopted to make a feed group, and the weight ratio of the artificial compound feed to the tubificidae section is 20:80, starting artificial bait domestication, feeding 8 times a day (feeding 1 time every 3 hours), wherein the feeding amount is 10wt% of the weight of the larvae and juvenile fish each time; after 5-7 days of domestication, increasing the weight ratio of the artificial mixed feed, simultaneously reducing the weight ratio of tubificidae sections, domesticating for 4 weeks, and then completely feeding the artificial mixed feed.
Further, in the step one (1), selecting female sturgeon with normal shape, normal body color and health without injury, wherein the weight of the female sturgeon is 4-6 kg/tail; selecting huso dauricus male fish with normal body color, large size, and health, and weight of 60-80 kg/tail.
Parent fish selection was performed at the beginning of 4 months per year.
Further, in the step one (2), the reinforced cultivation bait is prepared from the following raw materials in parts by weight: 10 parts of tenebrio molitor powder, 84 parts of artificially-mixed sturgeon feed (Acipenser sinensis feed), 0.5 part of vitamin C, 0.2 part of vitamin E, 0.3 part of taurine and 5 parts of vegetable powder.
Further, in the step one (3), the reproduction control of the parent fish is specifically as follows: when the natural water temperature is reduced to below 4 ℃, maintaining the water temperature of 4 ℃ to 30-60 days, then raising the water temperature from 4 ℃, slowly raising the water temperature by 1 ℃ every 3 days until the water temperature is raised to 14 ℃, then maintaining the water temperature unchanged, regularly observing the gonad development condition of the parent fish, and meeting the cultivation condition (continuously filling oxygen into the parent fish cultivation pond and cultivating in running water) and carrying out daily management (regularly cleaning the pond and changing water).
Further, in the step two (1), the oxytocic is LHRH-A 2 (luteinizing hormone releasing hormone for injection) and DOM (diosdone); the injection dosage of the acipenser baeri female fish is as follows: LHRH-A 2 6-10 mug/kg body weight and DOM 4mg/kg body weight, mixing and injecting; the injection dosage of the huso sturgeon oxytocic is reduced by half.
Further, in the second step (2), the specific operation steps of egg extruding and semen collecting are as follows:
fishing out huso dauricus male fish 10-12 hours after injection of oxytocic, squeezing while collecting semen with a sealing bag, storing at low temperature of 2-4 deg.C in a refrigerator for use, performing semen microscopic examination before artificial fertilization, selecting semen with strong sperm activity as qualified semen by microscopic examination, and waiting for artificial fertilization; then fishing the female sturgeon, extruding the eggs by a pressing method, and flowing into an egg containing basin to wait for fertilization.
Further, in the second step (3), the specific operation steps of artificial insemination are as follows:
adopting dry artificial insemination, performing microscopic examination on each kilogram of roe by using 15-20ml of qualified semen, putting the semen into an egg containing basin, uniformly stirring the semen with feathers, then adding hatching water (purified water) with the amount of 4 times that of the roe, continuously and rapidly stirring the mixture for 15s, activating the semen to fully combine the semen and the roe, standing the fertilized roe for 3-5min, removing sewage, and rinsing the roe (rinsing the roe for 3 times).
Further, in the third step, the weight ratio of the artificial compound feed to the tubificidae added in 4 weeks is as follows:
in week 1, the weight ratio of the artificial compound feed to the tubificidae segments is 40:60, adding a solvent to the mixture;
in week 2, the weight ratio of the artificial compound feed to the tubificidae segments is 60:40;
in week 3, the weight ratio of the artificial mixed feed to the tubificidae segments is 80:20;
in the 4 th week, the weight ratio of the artificial compound feed to the tubificidae sections is 100:0;
the feeding amount is 10wt% of the weight of the larvae and the young fishes each time, and the feeding is carried out for 8 times every day (the feeding is carried out once every 3 hours); after domestication for 4 weeks (the body length of the fish larvae is about 5 cm), the fish larvae grow into fish larvae, and the fish larvae completely eat artificial compound feed (the breeding density is changed into 800 tails/m) 2 ) (ii) a And (4) normally managing the fry in the fry stage, measuring the weight of the fry every 10 days, feeding the fry with feed according to 5wt% of the weight of the fry, and feeding the fry 3 times every day (feeding every 8 hours).
The invention adopts the huso bred in the second generation of China and the acipenser parvus as parents to breed hybrid sturgeons (male sturgeon and male huso sturgeon). Cultivation production practices show that the hybrid sturgeon fries of the small sturgeons and the huso have the advantages of fast growth, early sexual maturity, fine and smooth meat quality, easy domestication, strong disease resistance and the like, and show excellent meat quality hybrid dominant traits. Therefore, the hybrid seed production technology and the large-scale seed culture of the female acipenser parvum and the male huso have important research significance for promoting the healthy and sustainable development of the acipenser parvum industry in China.

Claims (8)

1. A method for breeding seedlings by hybridizing Acipenser parvum female fish and huso male fish is characterized in that: the method comprises the following steps:
step one, selecting, cultivating and reproductive control of parent fishes;
(1) Selecting parent fishes: selecting healthy acipenser parvum female fish and huso dauricus male fish, wherein the ages of the acipenser parvum female fish and the huso dauricus male fish are respectively more than 4 ages and 14 ages;
(2) Parent fish breeding: placing the selected female sturgeon and huso dauricus male fish into a parent fish culture pond, wherein the number ratio of the female and male parent fish is 3:1, feeding, namely feeding artificially-mixed sturgeon feed, wherein the feeding times are 2 times per day, and the daily feeding amount is 1.5% -2% of the total weight of male and female parent fishes;
when the gonad grows to the early stage IV, starting to perform nutrition-enhanced cultivation on the parent fish, feeding enhanced cultivation bait for 2 times a day, wherein the feeding amount per day is 1.5-2% of the total weight of the parent fish; feeding 2 times a day when gonad development reaches stage V, wherein the feeding amount is 1.5-2% of the total weight of parent fish; breeding parent fish before spawning and hatching;
(3) And (3) reproductive regulation: carrying out reproduction control on the parent fish in the parent fish gonad development process;
step two, artificial propagation;
(1) Artificial hastening parturition: when the gonad grows to the V stage, injecting oxytocic into the female sturgeon and the huso sturgeon in water at the temperature of 16-18 ℃;
(2) Egg extruding and semen collection:
(3) Artificial fertilization:
(4) And (3) debonding: pouring the washed fertilized eggs into a debonding device containing 10% of talcum powder aqueous solution, and debonding for 40-60min;
(5) Hatching fertilized eggs: after the debonded fertilized eggs are washed clean, the fertilized eggs are incubated, the water temperature is controlled to be 17.5-18 ℃, the pH value is 7-9, and after the fertilized eggs are incubated for 6-7 days, the young and the larval fish are hatched out by stripping;
step three, breeding the fish larvae and the juvenile fish;
culturing the newly-emerged fish larvae without feeding for 8-10 days in a culture pond, controlling the water temperature to be 17-20 ℃, controlling the pH to be 7-9 and controlling the dissolved oxygen to be more than or equal to 6mg/L, and carrying out running water or circulating water culture; after the larvae are opened, feeding tubificidae segments, wherein the feeding amount is 10wt% of the weight of the larvae every time, and feeding 8 times a day; after 7 days of opening, adopting special artificial mixed feed for sturgeon and tubificidae segments to make a feed group, wherein the weight ratio of the artificial mixed feed to the tubificidae segments is 20:80, starting artificial bait domestication, feeding 8 times a day, wherein the feeding amount is 10wt% of the weight of the larvae and the young fishes each time; after 5-7 days of domestication, increasing the weight ratio of the artificial mixed feed, simultaneously reducing the weight ratio of tubificidae sections, domesticating for 4 weeks, and then completely feeding the artificial mixed feed.
2. The method for cross breeding of Acipenser aethiopterus female fish and huso dauricus male fish according to claim 1, wherein the method comprises the following steps: in the first step (1), parent fish selection is carried out at the beginning of 5 months every year, and female sturgeon with normal shape, normal body color and health and no injury is selected, and the body weight reaches 4-6 kg/tail; selecting the huso dauricus male fish with normal body color, large size and health without injury, and the weight of the huso dauricus male fish reaches 60-80 kg/tail.
3. The method for cross breeding of Acipenser aethiopterus female fish and huso dauricus male fish according to claim 1, wherein the method comprises the following steps: in the step one (2), the reinforced cultivation bait is prepared from the following raw materials in parts by weight: 10 parts of tenebrio molitor powder, 84 parts of artificially-mixed sturgeon feed, 0.5 part of vitamin C, 0.2 part of vitamin E, 0.3 part of taurine and 5 parts of vegetable powder.
4. The method for cross breeding of Acipenser aethiopterus female fish and huso dauricus male fish according to claim 1, wherein the method comprises the following steps: in the step one (3), the reproduction control is carried out on the parent fish, and the method specifically comprises the following steps: when the natural water temperature is reduced to be below 4 ℃, maintaining the water temperature of 4 ℃ for 30-60 days, then raising the water temperature from 4 ℃ again, slowly raising the water temperature by 1 ℃ every 3 days until the water temperature is raised to 14 ℃, then maintaining the water temperature unchanged, regularly observing the gonad development condition of the parent fish, and meeting the cultivation condition and carrying out daily management.
5. The method for cross breeding of Acipenser aethiopterus female fish and huso dauricus male fish according to claim 1, wherein the method comprises the following steps: in the step two (1), the oxytocic is LHRH-A 2 And a DOM; the injection dosage of the acipenser baeri female fish is as follows: LHRH-A 2 Mixing 6-10 μ g/kg body weight, DOM 4mg/kg body weight, and injecting; the injection dosage of the huso sturgeon oxytocic is reduced by half.
6. The method for cross breeding of Acipenser aethiopterus female fish and huso dauricus male fish according to claim 1, wherein the method comprises the following steps: in the second step (2), the specific operation steps of egg extruding and semen collecting are as follows:
fishing out the huso dauricus male fish after injecting the oxytocic for 10-12 hours, squeezing and collecting semen with a sealing bag, storing at low temperature of 2-4 ℃ for later use, performing semen microscopic examination before artificial insemination, selecting the semen with strong sperm activity as the qualified semen through microscopic examination, and waiting for artificial insemination; then fishing the female sturgeon, extruding the eggs by a pressing method, and flowing into an egg containing basin to wait for fertilization.
7. The method for cross breeding of Acipenser parvum female fish and huso dauricus male fish according to claim 1, wherein: in the second step (3), the specific operation steps of artificial insemination are as follows:
adopting dry artificial insemination, examining qualified semen by using 15-20ml of microscope for each kilogram of roes, putting the semen into an egg containing basin, uniformly stirring the semen by using feathers, then adding hatching water with the amount 4 times that of the roes, continuously and rapidly stirring the semen for 15s, activating the semen to fully combine the sperm and the roes, standing the fertilized roes for 3-5min, discarding sewage, and rinsing the roes clean.
8. The method for cross breeding of Acipenser aethiopterus female fish and huso dauricus male fish according to claim 1, wherein the method comprises the following steps: in the third step, the weight ratio of the artificial compound feed to the tubificidae added in 4 weeks is as follows:
in week 1, the weight ratio of the artificial mixed feed to the tubificidae segments is 40:60, adding a solvent to the mixture;
in week 2, the weight ratio of the artificial compound feed to the tubificidae segments is 60:40;
in week 3, the weight ratio of the artificial compound feed to the tubificidae segments is 80:20;
in the 4 th week, the weight ratio of the artificial compound feed to the tubificidae sections is 100:0;
feeding the fish fries for 8 times every day, wherein the feeding amount of each time is 10wt% of the weight of the fish fries; after domestication for 4 weeks, the larvae and the juveniles grow into juvenile fishes, and the artificial compound feed is completely ingested; normally managing the fry in the fry stage, measuring the weight of the fry every 10 days, feeding the fry with feed according to 5wt% of the weight of the fry, and feeding the fry 3 times a day.
CN202211327432.4A 2022-10-27 2022-10-27 Acipenser parvum female fish and huso male fish hybrid breeding method Pending CN115530099A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202211327432.4A CN115530099A (en) 2022-10-27 2022-10-27 Acipenser parvum female fish and huso male fish hybrid breeding method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202211327432.4A CN115530099A (en) 2022-10-27 2022-10-27 Acipenser parvum female fish and huso male fish hybrid breeding method

Publications (1)

Publication Number Publication Date
CN115530099A true CN115530099A (en) 2022-12-30

Family

ID=84718652

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202211327432.4A Pending CN115530099A (en) 2022-10-27 2022-10-27 Acipenser parvum female fish and huso male fish hybrid breeding method

Country Status (1)

Country Link
CN (1) CN115530099A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116369250A (en) * 2023-03-16 2023-07-04 中国长江三峡集团有限公司中华鲟研究所 Acipenser dabryanus feeding training method

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116369250A (en) * 2023-03-16 2023-07-04 中国长江三峡集团有限公司中华鲟研究所 Acipenser dabryanus feeding training method
CN116369250B (en) * 2023-03-16 2023-12-12 中国长江三峡集团有限公司中华鲟研究所 Acipenser dabryanus feeding training method

Similar Documents

Publication Publication Date Title
CN102047851B (en) Construction and stock breeding method for cultured grass carp families
CN107549059B (en) Hybrid breeding method for female sturgeon and male paddlefish
CN101103706B (en) Reproduction method for American reeves shad artificial cultivation parent fish
CN110352877B (en) All-season artificial propagation method for micropterus salmoides
CN104719199A (en) Cross breeding method for pelteobagrus fulvidraco and pseudobagrus vachelli
CN103493757B (en) Method for breeding and cultivating four generations of fish and shrimp seedlings in pond in one year
CN102017921A (en) Siniperca chuatsi and siniperca kneri hybridization and culture method
CN101223865A (en) Seed production and culture method for cyprinoid with short growth circle
CN115530099A (en) Acipenser parvum female fish and huso male fish hybrid breeding method
CN1327763C (en) Method of establishing bred prawn pedigree and selectively breeding improved prawn variety
CN107155971A (en) Yi Zhong Gan fishes and black carp quasi aromatic hybrid rice breeding method
CN100512639C (en) Plecoglossus altivelis artificial reproduction method
CN106376500B (en) The thin squama schizothoracin artificial fecundation of Yalongjiang River releases method
US11547097B2 (en) Hybridization method of Sinocyclocheilus grahami and carp
CN112136728B (en) Artificial breeding method for first-filial generation parent fish of Gymnocypris duringii in circulating water system
CN111183929B (en) Erythroculter ilishaeformis induction pengze crucian gynogenesis method
CN104604790A (en) Method of breeding giant salamander in tunnels
CN110178766B (en) Artificial breeding method of Acipenser lapipenser
CN113331087A (en) Artificial propagation method of Gymnocypris ventricosa
CN106417117A (en) Yalong River schizothorax wangchiachii artificial proliferation and release method
US20220175507A1 (en) Artificial Propagation Method Of Sinocyclocheilus Rhinocerous
CN103518649A (en) Hephaestus fuliginosus and scortum barcoo outbreeding technology
CN104488781A (en) Breeding method for Chaohuwhitebait
CN106259126B (en) Yalongjiang River perch carp artificial fecundation releases method
CN104285869A (en) Method for improving female ratio of parabramis pekinensis

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination