CN101223865A - Seed production and culture method for cyprinoid with short growth circle - Google Patents
Seed production and culture method for cyprinoid with short growth circle Download PDFInfo
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Abstract
The invention discloses a seed production and culture method of a carp with short growth period and comprises the steps of the selection of breeding parent, which determines Jinxin female carp and Ukrainian scaled male carp as seed production parents through the RAPD technology, grouping of the male and female parents with a proportion of 1 to 1.2-1.5, reproduction of fish seeds by the methods of artificial flushing, natural spawning or artificial insemination, incubation in the step of which artificial fish egg collectors with naturally produced eggs and artificial fish egg collectors with fertilized ovum through artificial dousing are delivered to a pond for natural incubation or the fertilized ovum through artificial insemination is doused in the waxed pond bottom and then placed into a ring channel for incubation after being collected and washed, and initial culture and commercial fish culture: each Mu of pond can culture 2,000-2,200 pieces of summer fingerlings or 1200-1400 pieces of spring fingerlings and is matched with silver carp fingerlings or spring fingerlings which account for 20 percent of the main culture objects. The fish can be cultured in the current year. The carp with short growth period cultured by the method of the invention has the advantages of unique shape, rapid growth, strong disease resistance and high yield.
Description
Technical field
The present invention relates to aquaculture field, the production of hybrid seeds and the cultural method of the carp that especially a kind of growth cycle is short.
Background technology
Carp is traditional govern-house-variety that culturing area is the widest, area is maximum, gross yield is the highest in China's culture fishery, it also is the first kind of Tianjin freshwater aquiculture, according to statistics, Tianjin municipalization freshwater cultured area was more than 40 ten thousand mu in 2005, culturing gross yield is 27.7 ten thousand tons, and wherein carp output is 9.5 ten thousand tons, accounts for to culture 34% of gross yield, 12 districts all relate to carp culture, about 80,000 mu of intensive culture area.
But existing in the market carp fry kind quality is uneven, and kind is assorted, and proterties is degenerated, and has influenced the breeding production sale to a certain extent, and this just needs one can substitute local carp, colory carp new varieties.
Summary of the invention
Technical problem to be solved by this invention is, the production of hybrid seeds and the cultural method of the short carp of a kind of growth cycle are provided, by the genetic distance between 3 different cultivars carps of RAPD technical Analysis colony, therefrom find out a best of breed by artificial induced spawning, fertilization, hatching, seed rearing, the short carp of growth cycle of numerous advantages such as obtain form uniqueness, stabilization characteristics of genetics, growth is fast, premunition is strong and feeding habits are extensive.
In order to solve the problems of the technologies described above, the technical solution used in the present invention is: the production of hybrid seeds and the cultural method of the carp that a kind of growth cycle is short, comprise: production of hybrid seeds parent's seed selection, the combo of female male parent, seed production, hatching, the young pool of crow and marketable fish breed comprise following process successively:
(1) production of hybrid seeds parent's seed selection:, determine that therefrom two new carp rauns in carp kind Tianjin and Ukraine squama carp milter that genetic distance is far away are production of hybrid seeds parent by the genetic distance between 3 different cultivars carps of RAPD technical Analysis colony;
(2) combo of female male parent: the male and female ratio is 1: 1.2~1.5;
(3) seed produces: adopt artificial propagation, natural spawning or test-tube method
Artificial propagation:, obtain fully-developed ovum and sperm to parent population injection oxytocic drug;
Natural spawning: the parent population that will inject oxytocic drug is put into spawning pond, and miniflow water stimulates, and puts into artificial fish nest, induced spawning;
Or artificial insemination: when light pressure Tianjin new carp raun belly has the ovum grain to flow out parent population is caught out, extruding raun belly is extruded fully-developed ovum in the abdominal cavity, clamp-ons the seminal fluid of Ukraine squama carp milter, and smart ovum mixes fertilization;
(4) hatching: will be stained with the artificial fish nest of natural spawning and, change the pond over to and hatch naturally through manually splashing, be stained with the artificial fish nest of fertilized egg; Perhaps test-tube fertilized egg is splashed at the bottom of the spawning pond of handling through waxing, after collecting, washing, put into the circuit hatching;
(5) the young pool of sending out of crow: mu is put fry 200~3,000,000 tails, the soya bean of splashing slurry, and it is young to breed crow, and black son cultivates into summer flower or spring sheet;
(6) marketable fish is cultured: mu is put summer flower 2000~2200 tails or spring sheet fingerling 1200~1400 tails, and collocation is main breed fish 20% flower silver carp crow son or spring sheet fingerling formed then.
Described step (3) oxytocic drug is selected luteotropin releasing hormone d-ala analog LRH-A for use
2With maleic acid DOM DOM, the new carp raun in the every kg body weight of injected dose Tianjin is LRH-A
26.0ug+DOM4.0mg; Every kg body weight Ukraine squama carp milter consumption is 1/2 of a raun consumption.
Artificial insemination is in the described step (3): fully-developed ovum in the raun abdominal cavity is clamp-oned in the ceramic whiteware basin, clamp-oned the seminal fluid of milter, shake the porcelain basin smart ovum is fully contacted, fertilization 20~30s, pour in the water of temperature adjustment, make fertilized egg mix fertilization, hatch behind 3~10min.
Described step (5) is: black young high density is sent out the soya-bean milk of splashing during the pool, and every mu with soya bean 1.5~2.0kg, per 4~6 days new water 5~10cm that annotate.
The 750g adult fish is formed then, and mu is put summer flower 2000~2200 tails, per mu yield 1500~1800kg; Or the 1600g adult fish forms then, and mu is put spring sheet fingerling 1200~1400 tails, per mu yield 1800~2000kg; Daily ration of feeding is 3.5~6% of a pond fish TBW, raises 5~6 months.
Described step (1) requires more than the new carp raun body weight 2000g of Tianjin, more than the Ukraine squama carp milter body weight 1500g.
Described step (3) artificial propagation method is: to parent population injection oxytocic drug, the new carp raun in Tianjin is adopted double injection, and first pin is injected every kg body weight LRH-A
22.0ug second pin is injected every kg body weight LRH-A
24.0ug.Ukraine squama carp milter is adopted a shot, by every kg body weight LRH-A
26.0ug+DOM4.0mg mixed liquor, the position of injectable drug is the thoracic cavity of pectoral fin base portion, selects inject time, is injected at about 6 of mornings for the first time, injection for the second time was selected in about 6 pm, artificial insemination time o'clock is carried out the 2nd day morning 2~4.
The invention has the beneficial effects as follows: the short carp of growth cycle of the present invention has particular shape, biological character, growth is very fast, premunition is strong and feeding habits are extensive, the output advantages of higher, and through duplication of production and pond, different regions main support and raise together with in big waters, show stabilization characteristics of genetics, adaptability is strong, survival rate is high, its production of hybrid seeds parent's seed selection, the combo of female male parent and seed produce, cultural technique all is with a wide range of applications.
Embodiment
The production of hybrid seeds and the cultural method of the carp that growth cycle of the present invention is short comprise: production of hybrid seeds parent's seed selection, the combo of female male parent, seed production, hatching, crow son send out the pool and marketable fish breed, comprise following process successively:
(1) production of hybrid seeds parent's seed selection:, determine that therefrom two new carp rauns in carp kind Tianjin and Ukraine squama carp milter that genetic distance is far away are production of hybrid seeds parent by the genetic distance between 3 different cultivars carps of RAPD technical Analysis colony;
(2) combo of female male parent: the male and female ratio is 1: 1.2~1.5;
(3) seed produces: adopt artificial propagation, natural spawning or test-tube method
Artificial propagation:, obtain fully-developed ovum and sperm to parent population injection oxytocic drug;
Natural spawning: the parent population that will inject oxytocic drug is put into spawning pond, and miniflow water stimulates, and puts into artificial fish nest, induced spawning;
Or artificial insemination: when light pressure Tianjin new carp raun belly has the ovum grain to flow out parent population is caught out, extruding raun belly is extruded fully-developed ovum in the abdominal cavity, clamp-ons the seminal fluid of Ukraine squama carp milter, and smart ovum mixes fertilization;
(4) hatching: will be stained with the artificial fish nest of natural spawning and, change the pond over to and hatch naturally through manually splashing, be stained with the artificial fish nest of fertilized egg; Perhaps test-tube fertilized egg is splashed at the bottom of the spawning pond of handling through waxing, after collecting, washing, put into the circuit hatching;
(5) the young pool of sending out of crow: mu is put fry 200~3,000,000 tails, the soya bean of splashing slurry, and it is young to breed crow, and black son cultivates into summer flower or spring sheet;
(6) marketable fish is cultured: mu is put summer flower 2000~2200 tails or spring sheet fingerling 1200~1400 tails, and collocation is main breed fish 20% flower silver carp crow son or spring sheet fingerling formed then.
Described step (3) oxytocic drug is selected luteotropin releasing hormone d-ala analog LRH-A for use
2With maleic acid DOM DOM, the new carp raun in the every kg body weight of injected dose Tianjin is LRH-A
26.0ug+DOM4.0mg; Every kg body weight Ukraine squama carp milter consumption is 1/2 of a raun consumption.
Artificial insemination is in the described step (3): fully-developed ovum in the raun abdominal cavity is clamp-oned in the ceramic whiteware basin, clamp-oned the seminal fluid of milter, shake the porcelain basin smart ovum is fully contacted, fertilization 20~30s, pour in the water of temperature adjustment, make fertilized egg mix fertilization, hatch behind 3~10min.
Described step (5) is: black young high density is sent out the soya-bean milk of splashing during the pool, and every mu with soya bean 1.5~2.0kg, per 4~6 days new water 5~10cm that annotate.
The 750g adult fish is formed then, and mu is put summer flower 2000~2200 tails, per mu yield 1500~1800kg; Or the 1600g adult fish forms then, and mu is put spring sheet fingerling 1200~1400 tails, per mu yield 1800~2000kg; Daily ration of feeding is 3.5~6% of a pond fish TBW, raises 5~6 months.
Described step (1) requires more than the new carp raun body weight 2000g of Tianjin, more than the Ukraine squama carp milter body weight 1500g.
Described step (3) artificial propagation method is: to parent population injection oxytocic drug, the new carp raun in Tianjin is adopted double injection, and first pin is injected every kg body weight LRH-A
22.0ug second pin is injected every kg body weight LRH-A
24.0ug.Ukraine squama carp milter is adopted a shot, by every kg body weight LRH-A
26.0ug+DOM4.0mg mixed liquor, the position of injectable drug is the thoracic cavity of pectoral fin base portion, selects inject time, is injected at about 6 of mornings for the first time, injection for the second time was selected in about 6 pm, artificial insemination time o'clock is carried out the 2nd day morning 2~4.
Be elaborated below in conjunction with specific embodiment:
Example 1
The short carp production of hybrid seeds and the cultural method of growth cycle comprises:
1, production of hybrid seeds parent's seed selection
By the genetic distance between 3 different cultivars carps of RAPD technical Analysis colony, determine that therefrom--new carp raun in----Tianjin and Ukraine squama carp milter are production of hybrid seeds parent to genetic distance two carp kinds far away; The male and female production of hybrid seeds parent who selects the healthy and strong anosis nothing of physique to hinder from the reserve parent fish colony that cultivates requires more than the new carp raun body weight 2000g of Tianjin, more than the Ukraine squama carp milter body weight 1500g, carries out the raising of single pond and cultivates.
2, the combo of female male parent: the male and female ratio is 1: 1.2~1.5;
3, seed produces: adopt artificial propagation, the method that natural spawning or artificial insemination combine
(1) artificial propagation method: to parent population injection oxytocic drug, so that obtain fully-developed ovum and sperm, the new carp raun in Tianjin is adopted double injection, and first pin is injected every kg body weight LRH-A
22.0ug second pin is injected every kg body weight LRH-A
24.0ug.Ukraine squama carp milter is adopted a shot, by every kg body weight LRH-A
26.0ug+DOM4.0mg mixed liquor.The position of injectable drug is thoracic cavity (a pectoral fin base portion).Select inject time, is injected at about 6 of mornings for the first time; Injection for the second time was selected in about 6 pm, and artificial insemination time o'clock is carried out the 2nd day morning 2~4.
The key of artificial induced spawning is selection, reinforced cultivating and the artificial induced spawning technology of sexual maturity parent population.
(2) natural spawning: the parent population that will inject oxytocic drug (injecting method is with following) is put into spawning pond, and miniflow water stimulates, and puts into artificial fish nest, induced spawning;
(3) artificial insemination method: behind the parent population injection oxytocic drug, under the effect of oxytocic drug, the former intensive ovum that is wrapped in the fish nest is reached maturity and is fallen into the abdominal cavity, when having the ovum grain to flow out, light pressure Tianjin new carp raun belly should in time parent population be caught out, put into the fish folder, one people wraps tightly the fish folder, make fish belly portion down, head makes progress, and another people dries the water on the fish folder, and with the hands push the raun belly gently, fully-developed ovum in the abdominal cavity is clamp-oned in the clean ceramic whiteware basin smoothly, and the 3rd people clamp-ons the seminal fluid of Ukraine squama carp milter, and the 4th people shakes the ceramic whiteware basin gently makes smart ovum mix fertilization, be fertilized approximately behind 20~30s, pour into and meet the water supply standard and through the well water of temperature adjustment of breeding fish, another hand stretches in the basin water simultaneously, and water is rotated, fertilized egg is also rotated with water and in the floating water, smart ovum is fully contacted.Behind about 3~5min fertilized egg evenly splashed on the artificial fish nest at the bottom of being laid on the about 50cm of depth of water pond in advance or at the bottom of the spawning pond pond that waxing is handled.
4, scale hatching technique
Fertilized egg is adopted two kinds of hatching techniques: a kind of is to be stained with the artificial fish nest of natural spawning and through manually splashing, be stained with the artificial fish nest of fertilized egg, to change the pond over to and hatch naturally; Another kind is that fertilized egg is evenly splashed at the bottom of handling the spawning pond pond through waxing, again with fertilized egg with the bar broom sweep from, collect egg-collecting box reuse cluster current take off glutinous after, put into circuit and hatch.This dual mode, every batch of production, all more than 6000~8,000 ten thousand tails, once hatching all can reach 5000~7,000 ten thousand tails to every batch of production fry (spray).
5, the young pool of sending out of crow: mu is put fry 200~3,000,000 tails, and black young high density is sent out the soya-bean milk of splashing during the pool, and every mu with soya bean 1.5~2.0kg, per 4~6 days new water 5~10cm that annotate., it is young to breed crow, and black son cultivates into summer flower or spring sheet;
6, marketable fish is cultured: the 750g adult fish is formed then, and mu is put summer flower 2000~2200 tails, per mu yield 1500~1800kg; Or the 1600g adult fish forms then, and mu is put spring sheet fingerling 1200~1400 tails, per mu yield 1800~2000kg; Daily ration of feeding is 3.5~6% of a pond fish TBW, raises 5~6 months.
Example 2
Main form, the biological property of the carp that growth cycle is short are as follows:
(1) main morphological feature
The morphological feature of the carp that table 1 growth cycle is short
Project | Carp (the body long 291.0~445.0mm) that growth cycle is short | Project | Carp (the body long 291.0~445.0mm) that growth cycle is short |
Body length/height | 3.04±0.25 | Dorsal fin bar number | iii,18~21 |
Body length/body is wide | 5.62±0.56 | Pectoral fin bar number | i,15~18 |
Body length/head length | 4.56±0.47 | Abdomeinal fin bar number | ii,7~9 |
Head length/kiss is long | 3.87±0.54 | Stern fin ray number | iii,4~5 |
Head length/eye footpath | 4.31±0.38 | Left side mandibular arch gill raker number | 19~23 |
Head length/eye spacing | 5.74±0.58 | ||
Caudal peduncle length/caudal peduncle height | 1.37±0.15 |
(2) the main biological property of the carp of growth cycle weak point
The main biological property of the carp that table 2 growth cycle is short
Project | The carp that growth cycle is short |
Adapt to water temperature | 0~38℃ |
The optimum growh water temperature | 23~29℃ |
Oxygen-resistant ability | Can lower oxygen concentration resistance (it is fine to ingest when oxygen content 2~3mg/L) |
Premunition | Premunition is stronger |
Feeding habits | Adapt to artifical compound feed fully |
The short carp of growth cycle of the present invention is to hybridize the carp new varieties of the fast growth that obtains through the repetition tests in 3 years with two carp kinds of different affiliations.Novelty that the present invention has and superiority:
1. determine that by the RAPD technology new carp raun in Tianjin and Ukraine squama carp milter are production of hybrid seeds parent;
2. carry out hybrid seeding, obtain a stabilization characteristics of genetics, carp new varieties that premunition is strong, be applied to produce and obtained favorable economic benefit and social benefit;
3. short carp inheritance stability, the germplasm excellence of growth cycle.The carp that the growth cycle bred is short, filial generation is stable, growth is very fast, premunition is strong, raise survival rate height, lower oxygen concentration resistance, can the high density intensive culture, per mu yield can reach 1600~2000kg;
4. seed large-scale production.Present technique accompanies by the artificial induced spawning technology and makes it reach the synchronism maturation according to the natural law of female male parent gonad development, obtains the high fertilized egg of fertilization rate with technology of artificial insemination again.In order to reach this purpose, from the reserve parent fish colony that cultivates, to select goodly should produce the parent, and impose reinforced cultivating, this is the key that can seed large-scale production; Simultaneously, for the scale seed produces, adopt the hatching of circuit flowing water;
In sum, among the embodiment that content of the present invention is not confined to, the knowledgeable people in the same area can propose other embodiment easily within technological guidance's thought of the present invention, but this embodiment comprises within the scope of the present invention
Claims (7)
1. the production of hybrid seeds and the cultural method of the short carp of a growth cycle comprise: production of hybrid seeds parent's seed selection, the combo of female male parent, seed produce, hatching, crow are young sends out the pool and marketable fish is cultured, and it is characterized in that, comprises following process successively:
(1) production of hybrid seeds parent's seed selection:, determine that therefrom two new carp rauns in carp kind Tianjin and Ukraine squama carp milter that genetic distance is far away are production of hybrid seeds parent by the genetic distance between 3 different cultivars carps of RAPD technical Analysis colony;
(2) combo of female male parent: the male and female ratio is 1: 1.2~1.5;
(3) seed produces: adopt artificial propagation, natural spawning or test-tube method
Artificial propagation:, obtain fully-developed ovum and sperm to parent population injection oxytocic drug;
Natural spawning: the parent population that will inject oxytocic drug is put into spawning pond, and miniflow water stimulates, and puts into artificial fish nest, induced spawning;
Or artificial insemination: when light pressure Tianjin new carp raun belly has the ovum grain to flow out parent population is caught out, extruding raun belly is extruded fully-developed ovum in the abdominal cavity, clamp-ons the seminal fluid of Ukraine squama carp milter, and smart ovum mixes fertilization;
(4) hatching: will be stained with the artificial fish nest of natural spawning and, change the pond over to and hatch naturally through manually splashing, be stained with the artificial fish nest of fertilized egg; Perhaps test-tube fertilized egg is splashed at the bottom of the spawning pond of handling through waxing, after collecting, washing, put into the circuit hatching;
(5) the young pool of sending out of crow: mu is put fry 200~3,000,000 tails, the soya bean of splashing slurry, and it is young to breed crow, and black son cultivates into summer flower or spring sheet;
(6) marketable fish is cultured: mu is put summer flower 2000~2200 tails or spring sheet fingerling 1200~1400 tails, and collocation is main breed fish 20% flower silver carp crow son or spring sheet fingerling formed then.
2. according to the production of hybrid seeds and the cultural method of the short carp of the described growth cycle of claim 1, it is characterized in that described step (3) oxytocic drug is selected luteotropin releasing hormone d-ala analog LRH-A for use
2With maleic acid DOM DOM, the new carp raun in the every kg body weight of injected dose Tianjin is LRH-A
26.0ug+DOM4.0mg; Every kg body weight Ukraine squama carp milter consumption is 1/2 of a raun consumption.
3. according to the production of hybrid seeds and the cultural method of the short carp of the described growth cycle of claim 1, it is characterized in that, artificial insemination is in the described step (3): fully-developed ovum in the raun abdominal cavity is clamp-oned in the ceramic whiteware basin, clamp-on the seminal fluid of milter, shake the porcelain basin smart ovum is fully contacted, fertilization 20~30s pours in the water of temperature adjustment, make fertilized egg mix fertilization, hatch behind 3~10min.
4. according to the production of hybrid seeds and the cultural method of the short carp of the described growth cycle of claim 1, it is characterized in that described step (5) is: black young high density is sent out the soya-bean milk of splashing during the pool, and every mu with soya bean 1.5~2.0kg, per 4~6 days new water 5~10cm that annotate.
5. according to the production of hybrid seeds and the cultural method of the short carp of the described growth cycle of claim 1, it is characterized in that the 750g adult fish is formed then, mu is put summer flower 2000~2200 tails, per mu yield 1500~1800kg; Or the 1600g adult fish forms then, and mu is put spring sheet fingerling 1200~1400 tails, per mu yield 1800~2000kg; Daily ration of feeding is 3.5~6% of a pond fish TBW, raises 5~6 months.
6. according to the production of hybrid seeds and the cultural method of the short carp of the described growth cycle of claim 1, it is characterized in that described step (1) requires more than the new carp raun body weight 2000g of Tianjin, more than the Ukraine squama carp milter body weight 1500g.
7. according to the production of hybrid seeds and the cultural method of the short carp of the described growth cycle of claim 2, it is characterized in that described step (3) artificial propagation method is: to parent population injection oxytocic drug, the new carp raun in Tianjin is adopted double injection, and first pin is injected every kg body weight LRH-A
22.0ug second pin is injected every kg body weight LRH-A
24.0ug Ukraine squama carp milter is adopted a shot, by every kg body weight LRH-A
26.0ug+DOM4.0mg mixed liquor, the position of injectable drug is the thoracic cavity of pectoral fin base portion, selects inject time, is injected at about 6 of mornings for the first time, injection for the second time was selected in about 6 pm, artificial insemination time o'clock is carried out the 2nd day morning 2~4.
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Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
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CN102144591A (en) * | 2011-04-12 | 2011-08-10 | 中国水产科学研究院淡水渔业研究中心 | Method for breeding carp quickly and efficiently |
CN103168711A (en) * | 2011-12-24 | 2013-06-26 | 孔赟荣 | Breeding method of Russian carps |
CN103548743A (en) * | 2013-11-25 | 2014-02-05 | 永胜创意工贸有限公司 | Artificial propagation method for cyprinus chilia in Chenghai Lake |
CN105494208A (en) * | 2015-12-31 | 2016-04-20 | 山东省海洋生物研究院 | Sebastes schlegeli artificial squeezing fry breeding method |
CN107439424A (en) * | 2017-08-14 | 2017-12-08 | 天津市换新水产良种场 | A kind of production of hybrid seeds of Jin Xinwu crucian carps and cultural method |
CN108338096A (en) * | 2018-01-30 | 2018-07-31 | 平南县正达农业发展有限公司 | A method of improving carp Quality of Seedlings |
CN109845667A (en) * | 2019-04-17 | 2019-06-07 | 刘福安 | A kind of selection of carp new varieties |
CN112385585A (en) * | 2020-09-22 | 2021-02-23 | 杭州市农业科学研究院 | Artificial fish nest |
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2007
- 2007-12-29 CN CN2007100606064A patent/CN101223865B/en not_active Expired - Fee Related
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
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CN102144591A (en) * | 2011-04-12 | 2011-08-10 | 中国水产科学研究院淡水渔业研究中心 | Method for breeding carp quickly and efficiently |
CN102144591B (en) * | 2011-04-12 | 2012-09-05 | 中国水产科学研究院淡水渔业研究中心 | Method for breeding carp quickly and efficiently |
CN103168711A (en) * | 2011-12-24 | 2013-06-26 | 孔赟荣 | Breeding method of Russian carps |
CN103548743A (en) * | 2013-11-25 | 2014-02-05 | 永胜创意工贸有限公司 | Artificial propagation method for cyprinus chilia in Chenghai Lake |
CN105494208A (en) * | 2015-12-31 | 2016-04-20 | 山东省海洋生物研究院 | Sebastes schlegeli artificial squeezing fry breeding method |
CN107439424A (en) * | 2017-08-14 | 2017-12-08 | 天津市换新水产良种场 | A kind of production of hybrid seeds of Jin Xinwu crucian carps and cultural method |
CN108338096A (en) * | 2018-01-30 | 2018-07-31 | 平南县正达农业发展有限公司 | A method of improving carp Quality of Seedlings |
CN109845667A (en) * | 2019-04-17 | 2019-06-07 | 刘福安 | A kind of selection of carp new varieties |
CN112385585A (en) * | 2020-09-22 | 2021-02-23 | 杭州市农业科学研究院 | Artificial fish nest |
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