CN101213948A - Disease-resistant grass carp hybrid seeds production and cultivation method - Google Patents
Disease-resistant grass carp hybrid seeds production and cultivation method Download PDFInfo
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- CN101213948A CN101213948A CNA200710060605XA CN200710060605A CN101213948A CN 101213948 A CN101213948 A CN 101213948A CN A200710060605X A CNA200710060605X A CN A200710060605XA CN 200710060605 A CN200710060605 A CN 200710060605A CN 101213948 A CN101213948 A CN 101213948A
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- 241000252230 Ctenopharyngodon idella Species 0.000 title 1
- 238000012364 cultivation method Methods 0.000 title 1
- 201000010099 disease Diseases 0.000 title 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title 1
- 238000004519 manufacturing process Methods 0.000 title 1
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a fry production and culture method of disease-resistant grass carp. The invention consists of the breeding of fry production parents: excellent Squalibarbus curriculus spawner and grass carp milter are selected from rearing parents fish colony as the fry production parents and be reared in a single pool; the combination of female and male fish: the female and male ratio is 6-8:1; fry production and hatching: the method of combining artificial estrus induction with artificial insemination is adopted, and fertilized eggs are put in a circular hatchery; the cultivation of fish fry in ponds: 2-3 million fries are reared for each Mu, and the fries are reared by soybean milk; commercial fish cultivation: 12000 summer fingerlings or 2000 Common fingerlings are reared in each Mu, and collocating with main cultivated fish, 20 percent of bighead carps and silver carps fingerlings or Common fingerlings are reared. All the fish can grow up in the same year. The disease-resistant grass carp produced by the invention has the advantages of an unique form, a rapid growth speed, a strong disease-resistant capability, a wide food habit and a high survival rate.
Description
Technical field
The present invention relates to technical field of aquaculture, especially a kind of production of hybrid seeds of disease-resistant grass carp and cultural method.
Background technology
For many years; the disease of grass carp is to dampen the principal element of culturing peasant's enthusiasm, hindering the grass carp industry development all the time; therefore protect good germplasm and advanced breeding technique that the grass carp premunition is strengthened, make raiser's increasing both production and income, become the main task of breeding grass carp.
Summary of the invention
Technical problem to be solved by this invention is, a kind of production of hybrid seeds and cultural method of disease-resistant grass carp are provided, Squaliobarbus curriculus raun and the grass carp milter of selecting the healthy and strong anosis nothing of stabilization characteristics of genetics, physique to hinder from parental population carry out artificial induced spawning, insemination, hatching, seed rearing, thereby obtain form uniqueness, stabilization characteristics of genetics, the disease-resistant grass carp of numerous advantages such as growth is very fast, premunition is strong and feeding habits are extensive.
In order to solve the problems of the technologies described above, the technical solution used in the present invention is: a kind of production of hybrid seeds of disease-resistant grass carp and cultural method, comprise: production of hybrid seeds parent's seed selection, the combo of female male parent, seed produce and hatching, the young pool of crow and marketable fish are cultured, and comprise following process successively:
(1) production of hybrid seeds parent's seed selection: selecting good Squaliobarbus curriculus raun and grass carp milter from the reserve parent fish colony that cultivates is production of hybrid seeds parent, carries out single pond and raises cultivation;
(2) combo of female male parent: the male and female ratio is 6～8: 1;
(3) seed produces and hatching: the method that adopts artificial propagation and artificial insemination to combine
Artificial propagation:, obtain fully-developed ovum and sperm to parent population injection oxytocic drug;
Artificial insemination: when light pressure raun belly has the ovum grain to flow out parent population is caught out, extruding raun belly is extruded fully-developed ovum in the abdominal cavity, clamp-ons the seminal fluid of milter, and smart ovum mixes fertilization;
Hatching: test-tube fertilized egg is put into the circuit hatching;
(4) the black young pool of sending out: 5～10 mu of a pool pool areas, mu is put fry 200～3,000,000 tails, the soya bean slurry of splashing during the pool, the new water of annotating was bred the crow son in 10～14 days, and black son cultivates into summer flower or spring sheet;
(5) marketable fish is cultured: 10～20 mu of pond areas, the depth of water 0.8～1.0m in early stage, later stage 1.5～2.2m, mu is put summer flower 12000 tails or spring sheet fingerling 4000 tails, collocation is main breed fish 20% flower silver carp crow son or spring sheet fingerling, based on manufactured feed, the green forage of regularly throwing something and feeding was formed in 5～6 months during the breed.
Oxytocic drug is selected luteotropin releasing hormone d-ala analog LRH-A for use in the described step (3)
2With maleic acid DOM DOM, the every kg body weight of injected dose Squaliobarbus curriculus raun is luteotropin releasing hormone d-ala analog 6.0ug+ maleic acid DOM 4.0mg; Every kg body weight grass carp milter consumption is luteotropin releasing hormone d-ala analog 6.0ug.
Described step (3) is: fully-developed ovum in the raun abdominal cavity is clamp-oned in the ceramic whiteware basin, clamp-oned the seminal fluid of milter, shake the porcelain basin smart ovum is fully contacted, insemination 20～30s, pour in the water of temperature adjustment, it is floating that fertilized egg is rotated with water, behind 3～10min fertilized egg put into circular hatching channal and hatch.
Described step (4) is: black young high density is sent out the soya-bean milk of splashing during the pool, and every mu with soya bean 1.5～2.0kg, per 4～6 days new water 5～10cm that annotate.
The 100g disease-resistant grass carp is formed then, and mu is put young 12000 tails of crow, per mu yield 1000kg; The 1000g disease-resistant grass carp is formed method then, and mu is put spring sheet fingerling 2000 tails, per mu yield 1600～1800kg; Daily ration of feeding is 3.5～6% of a pond fish TBW, raises 5～6 months.
Described step (1) requires more than the Squaliobarbus curriculus raun body weight 300g, more than the grass carp milter body weight 5000g.
To parent population injection oxytocic drug, the Squaliobarbus curriculus raun is adopted a shot, by every kg body weight LRH-A in the described step (3)
26.0ug+DOM4.0mg mixed liquor; The grass carp milter is adopted double injection, and first pin is injected every kg body weight LRH-A
22.0ug second pin is injected every kg body weight LRH-A
24.0ug the position of injectable drug is the thoracic cavity of pectoral fin base portion; Select inject time, is injected at about 6 of mornings for the first time; Injection for the second time was selected in about 6 pm, and artificial insemination time o'clock is carried out the 2nd day morning 2～4.
The invention has the beneficial effects as follows: disease-resistant grass carp of the present invention has particular shape, biological character, growth is very fast, premunition strong and feeding habits characteristics widely, and through duplication of production and pond, different regions main support and raise together with in big waters, show stabilization characteristics of genetics, adaptability is strong, survival rate is high, the selection of its breeding parent, the combo of female male parent and seed produce, cultural technique all is with a wide range of applications.
Embodiment
The production of hybrid seeds of disease-resistant grass carp of the present invention and cultural method comprise: production of hybrid seeds parent's seed selection, the combo of female male parent, seed produce and hatching, the young pool of crow and marketable fish are cultured, and comprise following process successively:
(1) production of hybrid seeds parent's seed selection: selecting good Squaliobarbus curriculus raun and grass carp milter from the reserve parent fish colony that cultivates is production of hybrid seeds parent, carries out single pond and raises cultivation;
(2) combo of female male parent: the male and female ratio is 6～8: 1;
(3) seed produces and hatching: the method that adopts artificial propagation and artificial insemination to combine
Artificial propagation:, obtain fully-developed ovum and sperm to parent population injection oxytocic drug;
Artificial insemination: when light pressure raun belly has the ovum grain to flow out parent population is caught out, extruding raun belly is extruded fully-developed ovum in the abdominal cavity, clamp-ons the seminal fluid of milter, and smart ovum mixes fertilization;
Hatching: test-tube fertilized egg is put into the circuit hatching;
(4) the black young pool of sending out: 5～10 mu of a pool pool areas, mu is put fry 200～3,000,000 tails, the soya bean slurry of splashing during the pool, the new water of annotating was bred the crow son in 10～14 days, and black son cultivates into summer flower or spring sheet;
(5) marketable fish is cultured: 10～20 mu of pond areas, the depth of water 0.8～1.0m in early stage, later stage 1.5～2.2m, mu is put summer flower 12000 tails or spring sheet fingerling 4000 tails, collocation is main breed fish 20% flower silver carp crow son or spring sheet fingerling, based on manufactured feed, the green forage of regularly throwing something and feeding was formed in 5～6 months during the breed.
Oxytocic drug is selected luteotropin releasing hormone d-ala analog LRH-A for use in the described step (3)
2With maleic acid DOM DOM, the every kg body weight of injected dose Squaliobarbus curriculus raun is luteotropin releasing hormone d-ala analog 6.0ug+ maleic acid DOM 4.0mg; Every kg body weight grass carp milter consumption is luteotropin releasing hormone d-ala analog 6.0ug.
Described step (3) is: fully-developed ovum in the raun abdominal cavity is clamp-oned in the ceramic whiteware basin, clamp-oned the seminal fluid of milter, shake the porcelain basin smart ovum is fully contacted, insemination 20～30s, pour in the water of temperature adjustment, it is floating that fertilized egg is rotated with water, behind 3～10min fertilized egg put into circular hatching channal and hatch.
Described step (4) is: black young high density is sent out the soya-bean milk of splashing during the pool, and every mu with soya bean 1.5～2.0kg, per 4～6 days new water 5～10cm that annotate.
The 100g disease-resistant grass carp is formed then, and mu is put young 12000 tails of crow, per mu yield 1000kg; The 1000g disease-resistant grass carp is formed method then, and mu is put spring sheet fingerling 2000 tails, per mu yield 1600～1800kg; Daily ration of feeding is 3.5～6% of a pond fish TBW, raises 5～6 months.
Described step (1) requires more than the Squaliobarbus curriculus raun body weight 300g, more than the grass carp milter body weight 5000g.
To parent population injection oxytocic drug, the Squaliobarbus curriculus raun is adopted a shot, by every kg body weight LRH-A in the described step (3)
26.0ug+DOM4.0mg mixed liquor; The grass carp milter is adopted double injection, and first pin is injected every kg body weight LRH-A
22.0ug second pin is injected every kg body weight LRH-A
24.0ug the position of injectable drug is the thoracic cavity of pectoral fin base portion; Select inject time, is injected at about 6 of mornings for the first time; Injection for the second time was selected in about 6 pm, and artificial insemination time o'clock is carried out the 2nd day morning 2～4.
Be elaborated below in conjunction with specific embodiment:
Example 1
The production of hybrid seeds and the cultural method of disease-resistant grass carp comprise:
1. production of hybrid seeds parent's seed selection
The male and female production of hybrid seeds parent who selects the healthy and strong anosis nothing of physique to hinder from the reserve parent fish colony that cultivates requires more than the Squaliobarbus curriculus raun body weight 300g, more than the grass carp milter body weight 5000g, carries out the raising of single pond and cultivates.
2. the combo of female male parent: the male and female ratio is 6～8: 1;
3. seed produces
The method that adopts artificial propagation and artificial insemination to combine:
(1) artificial propagation method: to parent population injection oxytocic drug, so that obtain fully-developed ovum and sperm, the Squaliobarbus curriculus raun is adopted a shot, by every kg body weight LRH-A
26.0ug+DOM4.0mg mixed liquor.The grass carp milter is adopted double injection, and first pin is injected every kg body weight LRH-A
22.0ug second pin is injected every kg body weight LRH-A
24.0ug.The position of injectable drug is thoracic cavity (a pectoral fin base portion).Select inject time, is injected at about 6 of mornings for the first time; Injection for the second time was selected in about 6 pm, and artificial insemination time o'clock is carried out the 2nd day morning 2～4.
The key of artificial induced spawning is selection, reinforced cultivating and the artificial induced spawning technology of sexual maturity parent population.
(2) artificial insemination method: behind the parent population injection oxytocic drug, under the effect of oxytocic drug, the former intensive ovum that is wrapped in the fish nest is reached maturity and is fallen into the abdominal cavity, when having the ovum grain to flow out, light pressure raun belly should in time parent population be caught out, one human towel wraps tightly the fish body and blocks gonopore with finger, make fish belly portion down, head upwards, another people with the hands pushes the raun belly after drying the water that wraps up on the fish chaeta towel gently, and fully-developed ovum in the abdominal cavity is clamp-oned in the clean ceramic whiteware basin smoothly, the 3rd people clamp-ons the seminal fluid of grass carp milter, the 4th people shakes the ceramic whiteware basin gently fully contacts smart ovum, behind the 20～30s that inseminates approximately, pours into and meets the water supply standard and through the well water of temperature adjustment of breeding fish, another hand stretches in the basin water simultaneously, water is rotated, make fertilized egg, make smart ovum mix fertilization also with the water rotation and in the floating water.
4. scale hatching technique
Fertilized egg is adopted the circuit hatching, produce fry (spray) all at 6000～8,000 ten thousand tails for every batch, once hatching all can reach 6000～8,000 ten thousand tails, in the fertilized egg hatching process, note embryo development and water quality situation, the medical treatment of in time splashing is early molten, harmful organisms etc.
5. high density is sent out the pool
Send out 5～10 mu of pool pool areas, mu is put fry (spray) 200～3,000,000 tails, and every mu with soya bean 1.5～2.0kg, per 4～6 days new water 5～10cm that annotate, and it is young to breed crow in 10～14 days, and black son cultivates into summer flower or spring sheet;
6. marketable fish is cultured
Extremely strong, the lower oxygen concentration resistance of disease-resistant grass carp premunition adapts to artifical compound feed fully, is suitable for the crossbreed of high yielding culture.When culturing small dimension (100g) marketable fish, mu is put in a suitable place to breed down and is spent more than 12000 tails, and per mu yield can reach about 1000kg; When culturing the 1000g marketable fish, mu is put in a suitable place to breed more than spring sheet fingerling 2000 tails, and per mu yield can reach 1600～1800kg.Daily ration of feeding is 3.5～6% of a pond fish TBW, raises 5～6 months.
Fish disease did not take place during the breed, and the survival rate height is the new varieties of a suitable high-density breeding.
Example 2
The main form of disease-resistant grass carp, biological property are as follows:
(1) main morphological feature
The morphological feature at the disease-resistant careless angle of table 1
| Project | Disease-resistant grass carp (body long 155.9～224.2mm) | Project | Disease-resistant grass carp (body long 155.9～224.2mm) |
| Body length/height | 4.51±0.14 | Dorsal fin bar number | iii，7 |
| Body length/body is wide | 6.83±0.34 | Pectoral fin bar number | i，16～18 |
| Body length/head length | 4.78±0.20 | Abdomeinal fin bar number | ii，8 |
| Head length/kiss is long | 4.03±0.26 | Stern fin ray number | iii，8 |
| Head length/eye footpath | 4.31±0.38 | Left side mandibular arch gill raker number | 10～13 |
| Head length/eye spacing | 2.07±0.08 | ||
| Caudal peduncle length/caudal peduncle height | 1.46±0.09 |
(2) the main biological property of disease-resistant grass carp
The main biological property of table 2 disease-resistant grass carp
| Project | Disease-resistant grass carp |
| Adapt to water temperature | 0～38℃ |
| The optimum growh water temperature | 23～29℃ |
| Oxygen-resistant ability | Can lower oxygen concentration resistance (it is fine to ingest when oxygen content 2～3mg/L) |
| Premunition | Premunition is stronger |
| Feeding habits | Adapt to artifical compound feed fully |
The present invention is hybridized with two kinds of different affiliations, the strong grass carp of premunition that obtains through the repetition tests in 3 years.
Novelty that the present invention has and superiority:
1. two production of hybrid seeds parents' of disease-resistant grass carp selection.The application choice breeding technique selects the Squaliobarbus curriculus raun and the grass carp milter of stabilization characteristics of genetics, the healthy and strong anosis nothing wound of physique from parental population.
2. cross-breeding obtains a stabilization characteristics of genetics, grass carp new varieties that premunition is strong, is applied to produce and obtained favorable economic benefit and social benefit.
3. disease-resistant grass carp inheritance stability, germplasm excellence.The disease-resistant grass carp of breeding, filial generation is stable, growth is very fast, premunition is strong, raise survival rate height, lower oxygen concentration resistance, can the high density intensive culture, per mu yield can reach 1000～1800kg.
4. seed large-scale production.Present technique accompanies by the artificial induced spawning technology and makes it reach the synchronism maturation according to the natural law of female male parent gonad development, obtains the high fertilized egg of fertilization rate with technology of artificial insemination again.In order to reach this purpose, will from the reserve parent fish colony that cultivates, select goodly should produce the parent, and impose reinforced cultivating, this is the key that can seed large-scale production; Simultaneously, for the scale seed produces, adopt the hatching of circuit flowing water.
In sum, among the embodiment that content of the present invention is not confined to, the knowledgeable people in the same area can propose other embodiment easily within technological guidance's thought of the present invention, but this embodiment comprises within the scope of the present invention.
Claims (7)
1. the production of hybrid seeds of a disease-resistant grass carp and cultural method comprise: production of hybrid seeds parent's seed selection, the combo of female male parent, seed produce and hatching, crow are young sends out the pool and marketable fish is cultured, and it is characterized in that, comprises following process successively:
(1) production of hybrid seeds parent's seed selection: selecting good Squaliobarbus curriculus raun and grass carp milter from the reserve parent fish colony that cultivates is production of hybrid seeds parent, carries out single pond and raises cultivation;
(2) combo of female male parent: the male and female ratio is 6～8: 1;
(3) seed produces and hatching: the method that adopts artificial propagation and artificial insemination to combine
Artificial propagation:, obtain fully-developed ovum and sperm to parent population injection oxytocic drug;
Artificial insemination: when light pressure raun belly has the ovum grain to flow out parent population is caught out, extruding raun belly is extruded fully-developed ovum in the abdominal cavity, clamp-ons the seminal fluid of milter, and smart ovum mixes fertilization;
Hatching: test-tube fertilized egg is put into the circuit hatching;
(4) the black young pool of sending out: 5～10 mu of a pool pool areas, mu is put fry 200～3,000,000 tails, the soya bean slurry of splashing during the pool, the new water of annotating was bred the crow son in 10～14 days, and black son cultivates into summer flower or spring sheet;
(5) marketable fish is cultured: 10～20 mu of pond areas, the depth of water 0.8～1.0m in early stage, later stage 1.5～2.2m, mu is put summer flower 12000 tails or spring sheet fingerling 4000 tails, collocation is main breed fish 20% flower silver carp crow son or spring sheet fingerling, based on manufactured feed, the green forage of regularly throwing something and feeding was formed in 5～6 months during the breed.
2. according to the production of hybrid seeds and the cultural method of the described disease-resistant grass carp of claim 1, it is characterized in that oxytocic drug is selected luteotropin releasing hormone d-ala analog LRH-A for use in the described step (3)
2With maleic acid DOM DOM, the every kg body weight of injected dose Squaliobarbus curriculus raun is luteotropin releasing hormone d-ala analog 6.0ug+ maleic acid DOM 4.0mg; Every kg body weight grass carp milter consumption is luteotropin releasing hormone d-ala analog 6.0ug.
3. according to the production of hybrid seeds and the cultural method of the described disease-resistant grass carp of claim 1, it is characterized in that, described step (3) is: fully-developed ovum in the raun abdominal cavity is clamp-oned in the ceramic whiteware basin, clamp-on the seminal fluid of milter, shake the porcelain basin smart ovum is fully contacted, insemination 20～30s pours in the water of temperature adjustment, it is floating that fertilized egg is rotated with water, behind 3～10min fertilized egg put into circular hatching channal and hatch.
4. according to the production of hybrid seeds and the cultural method of the described disease-resistant grass carp of claim 1, it is characterized in that described step (4) is: black young high density is sent out the soya-bean milk of splashing during the pool, and every mu with soya bean 1.5～2.0kg, per 4～6 days new water 5～10cm that annotate.
5. according to the production of hybrid seeds and the cultural method of the described disease-resistant grass carp of claim 1, it is characterized in that: the 100g disease-resistant grass carp is formed then, and mu is put young 12000 tails of crow, per mu yield 1000kg; The 1000g disease-resistant grass carp is formed method then, and mu is put spring sheet fingerling 2000 tails, per mu yield 1600～1800kg; Daily ration of feeding is 3.5～6% of a pond fish TBW, raises 5～6 months.
6. according to the production of hybrid seeds and the cultural method of the described disease-resistant grass carp of claim 1, it is characterized in that described step (1) requires more than the Squaliobarbus curriculus raun body weight 300g, more than the grass carp milter body weight 5000g.
7. according to the production of hybrid seeds and the cultural method of the described disease-resistant grass carp of claim 1, it is characterized in that to parent population injection oxytocic drug, the Squaliobarbus curriculus raun is adopted a shot, by every kg body weight LRH-A in the described step (3)
26.0ug+DOM4.0mg mixed liquor; The grass carp milter is adopted double injection, and first pin is injected every kg body weight LRH-A
22.0ug second pin is injected every kg body weight LRH-A
24.0ug the position of injectable drug is the thoracic cavity of pectoral fin base portion; Select inject time, is injected at about 6 of mornings for the first time; Injection for the second time was selected in about 6 pm, and artificial insemination time o'clock is carried out the 2nd day morning 2～4.
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| CNA200710060605XA CN101213948A (en) | 2007-12-29 | 2007-12-29 | Disease-resistant grass carp hybrid seeds production and cultivation method |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102870730A (en) * | 2012-10-24 | 2013-01-16 | 衡东县土尚生态农业发展有限公司 | Grass carp breeding pond and method of breeding crisp grass carp in pond |
| CN103168711A (en) * | 2011-12-24 | 2013-06-26 | 孔赟荣 | Breeding method of Russian carps |
| CN105557570A (en) * | 2015-01-10 | 2016-05-11 | 赣州市水产研究所 | Method for increasing brood amount and spawning rate of parent ctenopharyngodon idellus |
| CN105613377A (en) * | 2016-01-08 | 2016-06-01 | 王芳 | Culture method of crisped grass carp |
| CN106912408A (en) * | 2017-03-03 | 2017-07-04 | 中国水产科学研究院黑龙江水产研究所 | A kind of salmon trout water resistant mildew new lines construction method |
| CN107439424A (en) * | 2017-08-14 | 2017-12-08 | 天津市换新水产良种场 | A kind of production of hybrid seeds of Jin Xinwu crucian carps and cultural method |
| CN111543390A (en) * | 2020-06-01 | 2020-08-18 | 湖南海博水产种业科技有限公司 | Preparation method and application of disease-resistant grass carp |
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2007
- 2007-12-29 CN CNA200710060605XA patent/CN101213948A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103168711A (en) * | 2011-12-24 | 2013-06-26 | 孔赟荣 | Breeding method of Russian carps |
| CN102870730A (en) * | 2012-10-24 | 2013-01-16 | 衡东县土尚生态农业发展有限公司 | Grass carp breeding pond and method of breeding crisp grass carp in pond |
| CN105557570A (en) * | 2015-01-10 | 2016-05-11 | 赣州市水产研究所 | Method for increasing brood amount and spawning rate of parent ctenopharyngodon idellus |
| CN105613377A (en) * | 2016-01-08 | 2016-06-01 | 王芳 | Culture method of crisped grass carp |
| CN106912408A (en) * | 2017-03-03 | 2017-07-04 | 中国水产科学研究院黑龙江水产研究所 | A kind of salmon trout water resistant mildew new lines construction method |
| CN106912408B (en) * | 2017-03-03 | 2020-04-21 | 中国水产科学研究院黑龙江水产研究所 | Construction method of novel salmon and trout anti-saprolegniasis strain |
| CN107439424A (en) * | 2017-08-14 | 2017-12-08 | 天津市换新水产良种场 | A kind of production of hybrid seeds of Jin Xinwu crucian carps and cultural method |
| CN111543390A (en) * | 2020-06-01 | 2020-08-18 | 湖南海博水产种业科技有限公司 | Preparation method and application of disease-resistant grass carp |
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