CN116369250B - Acipenser dabryanus feeding training method - Google Patents
Acipenser dabryanus feeding training method Download PDFInfo
- Publication number
- CN116369250B CN116369250B CN202310276491.1A CN202310276491A CN116369250B CN 116369250 B CN116369250 B CN 116369250B CN 202310276491 A CN202310276491 A CN 202310276491A CN 116369250 B CN116369250 B CN 116369250B
- Authority
- CN
- China
- Prior art keywords
- feeding
- tubificidae
- acipenser dabryanus
- acipenser
- seq
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000876433 Acipenser dabryanus Species 0.000 title claims abstract description 150
- 238000000034 method Methods 0.000 title claims abstract description 63
- 238000012549 training Methods 0.000 title claims abstract description 7
- 241000132906 Tubificidae Species 0.000 claims abstract description 94
- 230000008569 process Effects 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 43
- 239000002773 nucleotide Substances 0.000 claims description 40
- 125000003729 nucleotide group Chemical group 0.000 claims description 40
- 230000001954 sterilising effect Effects 0.000 claims description 18
- 150000003839 salts Chemical class 0.000 claims description 12
- 108020004414 DNA Proteins 0.000 claims description 11
- 230000002068 genetic effect Effects 0.000 claims description 11
- 239000000203 mixture Substances 0.000 claims description 11
- 238000012408 PCR amplification Methods 0.000 claims description 10
- 230000003203 everyday effect Effects 0.000 claims description 10
- 238000004659 sterilization and disinfection Methods 0.000 claims description 10
- 241000251468 Actinopterygii Species 0.000 claims description 9
- 230000002354 daily effect Effects 0.000 claims description 9
- 241000881711 Acipenser sturio Species 0.000 claims description 8
- 238000006243 chemical reaction Methods 0.000 claims description 8
- 238000007621 cluster analysis Methods 0.000 claims description 7
- 108091092878 Microsatellite Proteins 0.000 claims description 6
- 230000000249 desinfective effect Effects 0.000 claims description 6
- 210000003608 fece Anatomy 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 claims description 5
- 241000883303 Acipenser sinensis Species 0.000 claims description 4
- 238000000137 annealing Methods 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- 208000002109 Argyria Diseases 0.000 claims description 3
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 claims description 3
- 230000002776 aggregation Effects 0.000 claims description 3
- 238000004220 aggregation Methods 0.000 claims description 3
- 239000008280 blood Substances 0.000 claims description 3
- 210000004369 blood Anatomy 0.000 claims description 3
- 238000001962 electrophoresis Methods 0.000 claims description 3
- 229920002401 polyacrylamide Polymers 0.000 claims description 3
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 3
- 239000012498 ultrapure water Substances 0.000 claims description 3
- 238000011144 upstream manufacturing Methods 0.000 claims description 3
- 210000001325 yolk sac Anatomy 0.000 claims description 3
- 238000004458 analytical method Methods 0.000 claims description 2
- 241000252335 Acipenser Species 0.000 claims 1
- 238000011161 development Methods 0.000 abstract description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 2
- 238000009360 aquaculture Methods 0.000 abstract 1
- 244000144974 aquaculture Species 0.000 abstract 1
- 230000006806 disease prevention Effects 0.000 abstract 1
- 230000001737 promoting effect Effects 0.000 abstract 1
- 241001233061 earthworms Species 0.000 description 7
- 238000009395 breeding Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 238000012258 culturing Methods 0.000 description 2
- 238000004925 denaturation Methods 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000012447 hatching Effects 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 208000026350 Inborn Genetic disease Diseases 0.000 description 1
- 208000024556 Mendelian disease Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012257 pre-denaturation Methods 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a feeding domestication method for Acipenser dabryanus, and belongs to the field of aquaculture. The method comprises a tubificidae feeding stage, a feeding promoting stage and an artificial feed feeding stage. In each stage, the cultivation is carried out according to a certain feeding mode and stocking density, and daily management work such as disease prevention and the like is carried out regularly. The method also provides a method for avoiding disorder of different families of Acipenser dabryanus in the feeding-training process, and preventing weak family individuals from being mixed into a family group with better development to influence feeding training of the family with better development. The method can realize the feeding domestication problem of the Acipenser dabryanus, provides technical support for the artificial release and protection of the Acipenser dabryanus, and has great popularization value.
Description
Technical Field
The invention belongs to the field of aquatic animal artificial breeding, and in particular relates to a feeding method for Acipenser dabryanus.
Background
Acipenser dabryanus (academic name: acipenser dabryanus) is a fish of the sturgeon family. The data record that the parent fish of the wild Acipenser dabryanus was bred in 1976. Although the artificial propagation of Acipenser dabryanus has been successful for many years, no detailed report has been made about the feeding of Acipenser dabryanus larvae. The young Acipenser dabryanus is weak in constitution, easy to be ill and high in death rate, and the high-efficiency feeding method of Acipenser dabryanus can greatly improve the survival rate of the young Acipenser dabryanus. In addition, the Acipenser dabryanus can be faced with a plurality of problems in actual production, such as the phenomenon that a plurality of families of Acipenser dabryanus are bred, confusion occurs in the breeding process, the growth speed of offspring among different families is different, and hereditary diseases exist in some families. If different families are mixedly bred, the evaluation of the reproductive performance of each family is caused to deviate, and the breeding plan of the next generation of Acipenser dabryanus is further influenced. How to efficiently raise young Acipenser dabryanus has become a part of the important work of protecting Acipenser dabryanus.
Disclosure of Invention
The invention aims to provide a feeding method for Acipenser dabryanus, which can effectively improve the success rate of the Acipenser dabryanus feeding.
The technical scheme of the invention is as follows:
a method for feeding Acipenser dabryanus comprises a tubificidae feeding stage, a feeding attraction stage and a synthetic feed feeding stage. The feeding method of the tubificidae feeding stage comprises the following steps: when the yolk sac of the young Acipenser dabryanus after hatching disappears, the smashed tubificidae is fed into the Acipenser dabryanus culture pond. The length of the smashed tubificidae is 0.8-1 mm, and then the length of the smashed tubificidae is increased by 5-10% every day. When feeding Acipenser dabryanus, the inside of the tubificidae cannot be doped with blood of the tubificidae. The cultivation density of Acipenser dabryanus is calculated according to the surface area of the bottom of the cultivation pond, and the cultivation density is 300-500 tails/m 2 . The daily feeding amount of the tubificidae is 5-9% of the total weight of the Acipenser dabryanus. The times of feeding the tubificidae are 6-10 times per day. According to the aggregation amount of the young Acipenser dabryanus, the water earthworms are dispersed and fed into the water body of the culture pond. When the tubificidae is fed, the tubificidae is guaranteed to sink to the water bottom and cannot float on the surface of the water body. After the tubificidae is fed for 15 minutes, the residual bait of the tubificidae is discharged out of the culture pond. Before feeding the tubificidae, the feces of the Acipenser dabryanus are discharged out of the culture pond. When feeding the tubificidae, water and air should be stopped. After the feeding of the tubificidae is finished, water and air are immediately fed, and the water flow speed in the culture pond is 0.05-01 m/s. The water exchange amount per day is 15-25 times. When the individual difference of the Acipenser dabryanus in the same culture pond reaches the maximum individual and the minimum individual ratio to exceed 1.5:1, carrying out pond-separating cultivation, namely cultivating large-individual Acipenser dabryanus in the same cultivation pond, and cultivating small-individual Acipenser dabryanus in the same cultivation pond. And when the death rate of the Acipenser dabryanus in the culture pond exceeds 3% in the same day, disinfecting. The specific disinfection method is that the industrial salt is placed in the culture pond, so that the density of the industrial salt in the culture pond reaches 5-10kg/m 3 Sterilizing for 0.5-3 hr, and continuously sterilizing for 3 days. If the mortality rate is not improved, the sterilization is continued for three days after three days of stopping. The water temperature is controlled at 18-22 ℃.
The feeding stage is characterized in that when the death rate of the Acipenser dabryanus is high (reaching 7%) or the length of the Acipenser dabryanus exceeds 5 cm in the feeding stage of the tubificidae, the Acipenser dabryanus starts to enter the feeding stage for feeding. Bait fed during domestication is a mixture of tubificidae and synthetic feed. When feeding Acipenser dabryanus, mixing the water earthworms and the synthetic feed in a ratio of 9:1, and gradually reducing the ratio of the water earthworms to the synthetic feed by 10% every day. The specific mixing method comprises the following steps: and (3) repeatedly mixing a small amount of synthetic feed and dehydrated tubificidae, drying the moisture by using an electric fan during the process, then repeatedly mixing the mixture after adding a small amount of synthetic feed, continuously mixing the mixture after uniformly mixing, and adding a small amount of tubificidae for mixing after the electric fan dries the moisture. The method is followed until the tubificidae and the synthetic feed are added. Feeding Acipenser dabryanus after the tubificidae and the synthetic feed are mixed. A small amount of methods are adopted for feeding Acipenser dabryanus for many times. Feeding is carried out once every two hours, and the feeding amount of each time is 0.5-1% of the weight of the Acipenser dabryanus. The feeding times per day are 6-10 times. The daily feeding amount of the tubificidae is 5-9% of the total weight of the Acipenser dabryanus. The ratio of the tubificidae to the synthetic feed is reduced by 10% in the next day if the feeding condition of the Acipenser dabryanus on the day is good, and the ratio of the tubificidae to the synthetic feed on the previous day is kept for feeding in the next day if the feeding condition of the Acipenser dabryanus on the day is poor. After 15 minutes of feeding, the residual bait of the tubificidae is discharged out of the culture pond. Before feeding the tubificidae, the feces of the Acipenser dabryanus are discharged out of the culture pond. Feeding with a feeding deviceWhen the earthworms are used, water and gas should be stopped. After the feeding of the tubificidae is finished, water and air are fed immediately, and the water flow speed in the culture pond is 0.1-0.2 m/s. When the individual difference of the Acipenser dabryanus in the same culture pond reaches the maximum individual and the minimum individual ratio to exceed 1.5:1, carrying out pond-separating cultivation, namely cultivating large-individual Acipenser dabryanus in the same cultivation pond, and cultivating small-individual Acipenser dabryanus in the same cultivation pond. And when the death rate of the Acipenser dabryanus in the culture pond exceeds 3% in the same day, disinfecting. The specific disinfection method is that the industrial salt is placed in the culture pond, so that the density of the industrial salt in the culture pond reaches 5-10kg/m 3 Sterilizing for 0.5-3 hr, and continuously sterilizing for 3 days. If the mortality rate is not improved, the sterilization is continued for three days after three days of stopping. Young fish which are not domesticated successfully should be selected for separate feeding, and domesticated after physical recovery. The water temperature is controlled at 18-22 ℃.
The artificial feed feeding stage is to completely realize food domestication of the Acipenser dabryanus when the ratio of the tubificidae to the synthetic feed is reduced to 0, namely, all Acipenser dabryanus can completely ingest the synthetic feed. Feeding the Acipenser dabryanus with the synthetic feed every day. Feeding Acipenser dabryanus twice a day. The daily feeding amount of the feed is 4-5% of the total weight of the Acipenser dabryanus. The cultivation density of Acipenser dabryanus after successful feeding is 80-100 tails/m 2 。
The method also provides a method for avoiding disorder of different families of Acipenser dabryanus in the feeding-training process, and preventing weak family individuals from being mixed into a family group with better development to influence feeding training of the family with better development. The specific method is that when the individual difference of the Acipenser dabryanus in the same culture pond reaches the maximum individual and the minimum individual ratio to exceed 1.5:1, carrying out pond-separating cultivation, namely cultivating large-individual Acipenser dabryanus in the same cultivation pond, and cultivating small-individual Acipenser dabryanus in the same cultivation pond. However, in actual production, different families are cultivated in different cultivation tanks, and when the individuals with different sizes are picked in actual production, the phenomenon of disordered placement of the individuals with different families often occurs. When this occurs, 6 Acipenser dabryanus are randomly selected from the group to be placed, and then are randomly selected from each culture tank to be placedAnd 6, performing genetic relationship analysis on all the individuals finally, and identifying the Acipenser dabryanus group to be placed and which culture tank are of the same family. The specific identification method comprises the following steps: 1) Extracting DNA of the Acipenser sinensis individuals in the population to be placed and DNA of the Acipenser sinensis individuals in each culture tank to be placed; 2) Performing PCR amplification on the DNA of the Acipenser dabryanus to be detected by using the microsatellite primer provided by the invention; 3) Electrophoresis is carried out on PCR amplified products by polyacrylamide gel, silver staining is carried out, and the size of the amplified products of each sample to be detected on each microsatellite primer is read; 4) And (3) measuring genetic distances among individuals by using genetic software, drawing a UPGMA cluster analysis chart according to the genetic distances, and distinguishing individuals of different families according to the cluster analysis result. The reaction system of the PCR amplification is 15ul:10 XPCR Buffer 1.5ul,2.5mmol/L dNTP 0.5ul, mgCl 2 1.5ul, each of the upstream and downstream primers of the two pairs of primers was 0.5ul, taq enzyme was 0.2ul, DNA template was 2ul, and ultrapure water was 7.3ul. The PCR amplification reaction procedure is as follows: pre-denaturation at 94 ℃ for 3 min; then denaturation at 94℃for 30 seconds, annealing at 56℃for 30 seconds and extension at 72℃for 45 seconds for 35 cycles; finally, the mixture is extended at 72 ℃ for 10 minutes and stored at 4 ℃. The genetic analysis software is MEGA software. The primers are 10 pairs, and form a 5-group Acipenser dabryanus double PCR reaction system, and the specific primer group information is as follows:
the specific primer set information is as follows:
group 1:
1F is a nucleotide sequence shown in SEQ ID NO. 1; CTGTGTCTTCTGCTCCTGGT;
1R is a nucleotide sequence shown in SEQ ID NO. 2; GCATGTCAGGGCCGGTATTA;
2F is a nucleotide sequence shown in SEQ ID NO. 3; CCTGAAGGCTACCAGACCAC;
2R is a nucleotide sequence shown as SEQ ID NO. 4; AAGAGAGCTTTACCGCCTGG;
group 2:
3F is the nucleotide sequence shown in SEQ ID NO. 5; TCCCATGCTGCATTAAGCCA;
3R is a nucleotide sequence shown in SEQ ID NO. 6; TCACAGAGTACTGCTCCAGC;
4F is the nucleotide sequence shown in SEQ ID NO. 7; TGCTCTACGGTGGGTTTGTC;
4R is a nucleotide sequence shown in SEQ ID NO. 8; CAGACACAAGCCTGTCCCTT;
group 3:
5F is the nucleotide sequence shown in SEQ ID NO. 9; CATGACTGGACTGCTGTGGA;
5R is a nucleotide sequence shown as SEQ ID NO. 10; CAAACCACTTTGCCACATGC;
6F is the nucleotide sequence shown in SEQ ID NO. 11; ACTGAGCACCTGAAAACGGT;
6R is a nucleotide sequence shown as SEQ ID NO. 12; ACAATGTGCTTGTCCCCAGG;
group 4:
7F is the nucleotide sequence shown in SEQ ID NO. 13; GGATTGTGGGGAACATGGAGA;
7R is the nucleotide sequence shown in SEQ ID NO. 14; GCAACTGGCTTCATCGTAATGG;
8F is the nucleotide sequence shown in SEQ ID NO. 15; ATACAGCTGTGTCGGTGTGG;
8R is the nucleotide sequence shown in SEQ ID NO. 16; ATGCCGGTACCCGTACATTG;
group 5:
9F is the nucleotide sequence shown in SEQ ID NO. 17; CATGTTCATTGGGTCGGGGA;
9R is the nucleotide sequence shown in SEQ ID NO. 18; GGAGATACACATTCAAAGACACGG;
10F is the nucleotide sequence shown in SEQ ID NO. 19; CTGTCAGGCAGCACAAAAGG;
10R is the nucleotide sequence shown in SEQ ID NO. 20; TCAGTGTGTGTGATGAAGGCA.
The primer annealing temperature was 57 ℃.
Compared with the prior art, the invention has the following advantages:
the method provided by the invention is developed in the process of raising Acipenser dabryanus in the team for years, can enable Acipenser dabryanus to successfully realize feeding, greatly improves the survival rate of Acipenser dabryanus young fish, and has a great popularization value. The method can also be used for identifying families, and avoids the phenomenon of family confusion of Acipenser dabryanus in the cultivation process.
Drawings
FIG. 1 results of individual cluster analysis of different families.
Detailed Description
In order to better understand the technical solutions of the present invention, the applicant will further describe the present invention in detail with reference to examples and drawings.
Example 1
The Acipenser dabryanus research institute of the Yangtze Sanxia group limited company of China successfully realizes the artificial reproduction of Acipenser dabryanus again in 4 months of 2021. Wherein, tens of thousands of young Acipenser dabryanus are transported to the Yangtze river rare fish conservation center for domestication and breeding, and the batch of fish is bred by 3 isotactic families. The culture pond is a culture pond with the diameter of 4.5 meters and the height of 1.5 meters. 0.8 ten thousand-tail Acipenser dabryanus young fish are raised in each culture pond. The water temperature is controlled at 18-22 ℃. Scientific researchers divide the feeding of Acipenser dabryanus into a tubificidae feeding stage, a feeding attracting stage and a synthetic feed feeding stage.
The feeding method in the feeding stage comprises the following steps: when the yolk sac of the young Acipenser dabryanus after hatching disappears, the smashed tubificidae is fed into the Acipenser dabryanus culture pond. The length of the smashed tubificidae is 0.8-1 mm, and then the length of the smashed tubificidae is increased by 5-10% every day. When feeding Acipenser dabryanus, the inside of the tubificidae cannot be doped with blood of the tubificidae. The cultivation density of Acipenser dabryanus is calculated according to the surface area of the bottom of the cultivation pond, and the cultivation density is 300-500 tails/m 2 . The daily feeding amount of the tubificidae is 5-9% of the total weight of the Acipenser dabryanus. The times of feeding the tubificidae are 6-10 times per day. According to the aggregation amount of the young Acipenser dabryanus, the water earthworms are dispersed and fed into the water body of the culture pond. When the tubificidae is fed, the tubificidae is guaranteed to sink to the water bottom and cannot float on the surface of the water body. After the tubificidae is fed for 15 minutes, the residual bait of the tubificidae is discharged out of the culture pond. Before feeding the tubificidae, the feces of the Acipenser dabryanus are discharged out of the culture pond. When feeding the tubificidae, water and gas are stopped. After the feeding of the tubificidae is finished, water and air are fed immediately, and the water flow speed in the culture pond is 0.05-0.1 m/s. The water exchange amount per day is 15-25 times. When the individual difference of the Acipenser dabryanus in the same culture pond reaches the maximum individual and the minimum individual ratio to exceed 1.5:1, then dividing the poolCulturing, namely culturing large-individual Acipenser dabryanus in the same culture pond, and culturing small-individual Acipenser dabryanus in the same culture pond. And when the death rate of the Acipenser dabryanus in the culture pond exceeds 3% in the same day, disinfecting. The specific disinfection method is that the industrial salt is placed in the culture pond, so that the density of the industrial salt in the culture pond reaches 5-10kg/m 3 Sterilizing for 0.5-3 hr, and continuously sterilizing for 3 days. If the mortality rate is not improved, the sterilization is continued for three days after three days of stopping. The water temperature is controlled at 18-22 ℃.
The feeding stage is that when the death rate of the Acipenser dabryanus is high (reaching 7%) or the length of the Acipenser dabryanus exceeds 5 cm in the feeding stage of the tubificidae, the Acipenser dabryanus starts to enter the feeding stage for feeding. Bait fed during domestication is a mixture of tubificidae and synthetic feed. When feeding Acipenser dabryanus, mixing the water earthworms and the synthetic feed in a ratio of 9:1, and gradually reducing the ratio of the water earthworms to the synthetic feed by 10% every day. The specific mixing method comprises the following steps: and (3) repeatedly mixing a small amount of synthetic feed and dehydrated tubificidae, drying the moisture by using an electric fan during the process, then repeatedly mixing the mixture after adding a small amount of synthetic feed, continuously mixing the mixture after uniformly mixing, and adding a small amount of tubificidae for mixing after the electric fan dries the moisture. The method is followed until the tubificidae and the synthetic feed are added. Feeding Acipenser dabryanus after the tubificidae and the synthetic feed are mixed. A small amount of methods are adopted for feeding Acipenser dabryanus for many times. Feeding is carried out once every two hours, and the feeding amount of each time is 0.5-1% of the weight of the Acipenser dabryanus. The feeding times per day are 6-10 times. The daily feeding amount of the tubificidae is 5-9% of the total weight of the Acipenser dabryanus. The ratio of the tubificidae to the synthetic feed is reduced by 10% in the next day if the feeding condition of the Acipenser dabryanus on the day is good, and the ratio of the tubificidae to the synthetic feed on the previous day is kept for feeding in the next day if the feeding condition of the Acipenser dabryanus on the day is poor. After 15 minutes of feeding, the residual bait of the tubificidae is discharged out of the culture pond. Before feeding the tubificidae, the feces of the Acipenser dabryanus are discharged out of the culture pond. When feeding the tubificidae, water and air should be stopped. After the feeding of the tubificidae is finished, water and air are fed immediately, and the water flow speed in the culture pond is 0.1-0.2 m/s. When the same culture pondThe individual difference of Acipenser dabryanus reaches the maximum individual to minimum individual ratio exceeding 1.5:1, carrying out pond-separating cultivation, namely cultivating large-individual Acipenser dabryanus in the same cultivation pond, and cultivating small-individual Acipenser dabryanus in the same cultivation pond. And when the death rate of the Acipenser dabryanus in the culture pond exceeds 3% in the same day, disinfecting. The specific disinfection method is that the industrial salt is placed in the culture pond, so that the density of the industrial salt in the culture pond reaches 5-10kg/m 3 Sterilizing for 0.5-3 hr, and continuously sterilizing for 3 days. If the mortality rate is not improved, the sterilization is continued for three days after three days of stopping. Young fish which are not domesticated successfully should be selected for separate feeding, and domesticated after physical recovery. The water temperature is controlled at 18-22 ℃.
The artificial feed feeding stage is to completely realize food domestication of the Acipenser dabryanus when the ratio of the tubificidae to the synthetic feed is reduced to 0, namely, all Acipenser dabryanus can completely ingest the synthetic feed. Feeding the Acipenser dabryanus with the synthetic feed every day. Feeding Acipenser dabryanus twice a day. The daily feeding amount of the feed is 4-5% of the total weight of the Acipenser dabryanus. The cultivation density of Acipenser dabryanus after successful feeding is 80-100 tails/m 2 . And finally, successfully realizing feeding domestication of all the Acipenser dabryanus.
6 Acipenser dabryanus and 18 Acipenser dabryanus are randomly extracted from the three families respectively, wherein 1-6 are the same family, 7-12 are the same family, and 13-18 are the same family. The 18-tail Acipenser dabryanus DNA is extracted, and the PCR amplification is carried out on each individual DNA by using the primers in the method, wherein the specific primer group information is as follows:
the reaction system for PCR amplification was 15ul:10 XPCR Buffer 1.5ul,2.5mmol/L dNTP 0.5ul, mgCl 2 1.5ul, each of the upstream and downstream primers of the two pairs of primers was 0.5ul, taq enzyme was 0.2ul, DNA template was 2ul, and ultrapure water was 7.3ul. The PCR amplification reaction procedure was: pre-transformation at 94 DEG CSex for 3 minutes; then denaturation at 94℃for 30 seconds, annealing at 56℃for 30 seconds and extension at 72℃for 45 seconds for 35 cycles; finally, the mixture is extended at 72 ℃ for 10 minutes and stored at 4 ℃.
And (3) electrophoresis is carried out on PCR amplification products by using polyacrylamide gel, silver staining is carried out, and the size of the amplified products of each sample to be detected on each microsatellite primer is read. And (3) measuring genetic distances among individuals by using genetic software MEGA, drawing a UPGMA cluster analysis chart according to the genetic distances, and distinguishing individuals of different families according to cluster analysis results. The specific results are shown in FIG. 1. From the results of the graph, it can be seen that individuals 1 to 6 are grouped into the same branch, individuals 7 to 12 are grouped into the same branch, and individuals 13 to 18 are grouped into the same branch. The result proves that the method can realize the identification of families and can avoid the phenomenon that different families are mixedly cultured.
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should make equivalent substitutions or modifications according to the technical scheme of the present invention and the inventive concept thereof, and should be covered by the scope of the present invention.
Claims (9)
1. The method for feeding Acipenser dabryanus is characterized by comprising a tubificidae feeding stage, a feeding attraction stage and a synthetic feed feeding stage; the feeding method of the tubificidae feeding stage comprises the following steps:
1.1 when the yolk sac of the young Acipenser dabryanus to be hatched is disappeared, feeding smashed tubificidae into an Acipenser dabryanus culture pond, wherein the smashed tubificidae is 0.8-1 mm in length, and the smashed tubificidae is increased by 5-10% every day later; when feeding Acipenser dabryanus, the inside of the tubificidae cannot be doped with blood of the tubificidae;
1.2 Acipenser dabryanus cultivation Density calculated according to the surface area of the cultivation pond bottom, cultivation Density of 300-500 tails/m 2 The method comprises the steps of carrying out a first treatment on the surface of the The daily feeding amount of the tubificidae is 5-9% of the total weight of the Acipenser dabryanus, and the daily feeding times of the tubificidae are 6-10;
1.3, feeding tubificidae in the water body of the culture pond in a dispersing way according to the aggregation amount of the young Acipenser dabryanus; when the tubificidae is fed, the tubificidae is guaranteed to sink at the bottom of water and cannot float on the surface of the water body; after the tubificidae is fed for 15 minutes, the residual bait of the tubificidae is discharged out of the culture pond, and the excrement of the Acipenser dabryanus is discharged out of the culture pond before the tubificidae is fed;
1.4, when the tubificidae is fed, water and gas should be stopped, water and gas are immediately fed after the feeding of the tubificidae is finished, the water flow speed in the culture pond is 0.05-0.1 m/s, and the water exchange amount per day is 15-25 times;
1.5 when the individual difference of Acipenser dabryanus in the same culture pond reaches the maximum individual and the minimum individual ratio to exceed 1.5:1, carrying out pond-separating cultivation, namely cultivating large-individual Acipenser dabryanus in the same cultivation pond, and cultivating small-individual Acipenser dabryanus in the same cultivation pond;
1.6 when the death rate of Acipenser dabryanus in the culture pond exceeds 3% on the same day, disinfecting: the specific disinfection method is that the industrial salt is placed in the culture pond, so that the density of the industrial salt in the culture pond reaches 5-10kg/m 3 Sterilizing for 0.5-3 hr, continuously sterilizing for 3 days, stopping for three days if death rate is not improved, and continuously sterilizing for three days with water temperature controlled at 18-22deg.C.
2. The method for feeding Acipenser dabryanus according to claim 1, wherein the feeding stage is started when the death rate of Acipenser dabryanus is more than or equal to 7% or the length of Acipenser dabryanus exceeds 5 cm in the feeding stage of tubificidae.
3. A method of feeding a sturgeon according to claim 2, wherein the method of the feeding stage comprises the following steps:
3.1 The bait fed during domestication is a mixture of tubificidae and synthetic feed, when the Acipenser dabryanus is fed, the tubificidae and the synthetic feed are mixed in a ratio of 9:1, the tubificidae and the synthetic feed are mixed in a ratio of 8:2 the next day, and the ratio of the tubificidae to the synthetic feed is gradually reduced by 10% every day after the ratio;
3.2, when feeding Acipenser dabryanus, adopting a few methods for many times: feeding once every two hours, wherein the feeding amount of each time is 0.5-1% of the weight of the Acipenser dabryanus, the feeding times per day is 6-10 times, and the feeding amount of tubificidae per day is 5-9% of the total weight of the Acipenser dabryanus; reducing the ratio of the tubificidae to the synthetic feed by 10% in the next day if the feeding condition of the Acipenser dabryanus in the day is good, and maintaining the ratio of the tubificidae to the synthetic feed in the previous day for feeding in the next day if the feeding condition of the Acipenser dabryanus in the day is poor; after 15 minutes of feeding, the residual bait of the tubificidae is discharged out of the culture pond; before feeding the tubificidae, the feces of the Acipenser dabryanus are discharged out of the culture pond;
3.3, when the mixture of the tubificidae and the synthetic feed is fed, water and air should be stopped, and after the mixture of the tubificidae and the synthetic feed is fed, water and air are immediately fed, and the water flow speed in the culture pond is 0.1-0.2 m/s;
3.4 when the individual difference of Acipenser dabryanus in the same culture pond reaches the maximum individual and the minimum individual ratio to exceed 1.5:1, carrying out pond-separating cultivation, namely cultivating large-individual Acipenser dabryanus in the same cultivation pond, and cultivating small-individual Acipenser dabryanus in the same cultivation pond;
3.5 when the death rate of the Acipenser dabryanus in the culture pond exceeds 3% on the same day, disinfecting: the specific disinfection method is that the industrial salt is placed in the culture pond, so that the density of the industrial salt in the culture pond reaches 5-10kg/m 3 Sterilizing for 0.5-3 hours, continuously sterilizing for 3 days, and continuously sterilizing for three days after stopping for three days if the death rate is not improved;
3.6, selecting young fish which are not domesticated successfully, feeding alone, domesticating after physical constitution is recovered, and controlling the water temperature at 18-22 ℃.
4. The method for feeding training sturgeon according to claim 3, wherein the mixing method of the tubificidae and the synthetic feed in the step 3.1 is as follows: repeatedly mixing synthetic feed and dehydrated tubificidae, drying moisture in the process, adding the synthetic feed, repeatedly mixing, adding tubificidae for continuous mixing after uniformly mixing, adding the synthetic feed for mixing after drying the moisture, and feeding Acipenser dabryanus after the tubificidae and the synthetic feed are mixed according to the method until the tubificidae and the synthetic feed are added.
5. The method for feeding the Acipenser dabryanus according to claim 1, wherein the artificial synthetic feed feeding stage is that when the ratio of tubificidae to synthetic feed is reduced to 0, namely, all the Acipenser dabryanus can fully ingest the synthetic feed, the food feeding of the Acipenser dabryanus is fully realized, the Acipenser dabryanus is fed with the synthetic feed every day, the Acipenser dabryanus is fed twice every day, the daily feeding amount of the feed is 4-5% of the total weight of the Acipenser dabryanus, and the cultivation density of the Acipenser dabryanus after successful feeding is 80-100 pieces/m 2 。
6. The method for feeding a sturgeon according to claim 1, further comprising a method for avoiding confusion of different families of the sturgeon during feeding, preventing weak family individuals from being mixed into a better-developed family group, and affecting feeding of the better-developed family.
7. The method for feeding a sturgeon according to claim 6, wherein the method for avoiding confusion of different sturgeon families is specifically that when the individual difference of the sturgeons in the same culture pond reaches the maximum individual to minimum individual ratio exceeding 1.5: when the method is used, large-individual Acipenser dabryanus is cultivated in the same cultivation pond, small-individual Acipenser dabryanus is cultivated in the same cultivation pond, but in actual production, different families are cultivated in different cultivation tanks, when the actual production of different-sized individuals is selected, the phenomenon that different families are placed in an individual mode often occurs, when the phenomenon occurs, 6 Acipenser dabryanus is randomly selected from a group to be placed, then 6 Acipenser dabryanus is randomly selected from each cultivation tank to be placed, and finally, all the individuals perform genetic relationship analysis to identify the Acipenser dabryanus group to be placed and which cultivation tank is the same family.
8. The method for feeding a sturgeon according to claim 7, wherein the specific identification method of the genetic relationship of the individual is as follows:
1) Extracting DNA of the Acipenser sinensis individuals in the population to be placed and DNA of the Acipenser sinensis individuals in each culture tank to be placed;
2) Performing PCR amplification on the Acipenser dabryanus DNA to be detected by using microsatellite primers;
3) Electrophoresis is carried out on PCR amplified products by polyacrylamide gel, silver staining is carried out, and the size of the amplified products of each sample to be detected on each microsatellite primer is read;
4) Measuring genetic distances among individuals by using genetic analysis software, drawing a UPGMA cluster analysis chart according to the genetic distances, and distinguishing individuals of different families according to cluster analysis results;
the reaction system of the PCR amplification is 15ul:10 XPCR Buffer 1.5ul,2.5mmol/L dNTP 0.5ul, mgCl 2 1.5ul, 0.5ul of each of the upstream and downstream primers of the two pairs of primers, 0.2ul of Taq enzyme, 2ul of DNA template, and 7.3ul of ultrapure water;
the PCR amplification reaction procedure is as follows: pre-denaturing at 94℃for 3 min, then denaturing at 94℃for 30 seconds, annealing at 56℃for 30 seconds, and extending at 72℃for 45 seconds for a total of 35 cycles; finally, the sample is extended at 72 ℃ for 10 minutes and stored at 4 ℃, and the genetic analysis software is MEGA software.
9. The feeding method for Acipenser dabryanus according to claim 8, wherein 10 pairs of microsatellite primers in the step 2) form 5 groups of Acipenser dabryanus double PCR reaction systems, and specific primer group information is as follows:
group 1:
1F is a nucleotide sequence shown in SEQ ID NO. 1;
1R is a nucleotide sequence shown in SEQ ID NO. 2;
2F is a nucleotide sequence shown in SEQ ID NO. 3;
2R is a nucleotide sequence shown as SEQ ID NO. 4;
group 2:
3F is the nucleotide sequence shown in SEQ ID NO. 5;
3R is a nucleotide sequence shown in SEQ ID NO. 6;
4F is the nucleotide sequence shown in SEQ ID NO. 7;
4R is a nucleotide sequence shown in SEQ ID NO. 8;
group 3:
5F is the nucleotide sequence shown in SEQ ID NO. 9;
5R is a nucleotide sequence shown as SEQ ID NO. 10;
6F is the nucleotide sequence shown in SEQ ID NO. 11;
6R is a nucleotide sequence shown as SEQ ID NO. 12;
group 4:
7F is the nucleotide sequence shown in SEQ ID NO. 13;
7R is the nucleotide sequence shown in SEQ ID NO. 14;
8F is the nucleotide sequence shown in SEQ ID NO. 15;
8R is the nucleotide sequence shown in SEQ ID NO. 16;
group 5:
9F is the nucleotide sequence shown in SEQ ID NO. 17;
9R is the nucleotide sequence shown in SEQ ID NO. 18;
10F is the nucleotide sequence shown in SEQ ID NO. 19;
10R is the nucleotide sequence shown in SEQ ID NO. 20.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310276491.1A CN116369250B (en) | 2023-03-16 | 2023-03-16 | Acipenser dabryanus feeding training method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310276491.1A CN116369250B (en) | 2023-03-16 | 2023-03-16 | Acipenser dabryanus feeding training method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN116369250A CN116369250A (en) | 2023-07-04 |
| CN116369250B true CN116369250B (en) | 2023-12-12 |
Family
ID=86962648
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310276491.1A Active CN116369250B (en) | 2023-03-16 | 2023-03-16 | Acipenser dabryanus feeding training method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116369250B (en) |
Citations (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004068941A1 (en) * | 2003-01-27 | 2004-08-19 | Davis Russell P | Integrated system of shellfish production and utilization |
| CN101326899A (en) * | 2007-06-22 | 2008-12-24 | 中国水产科学研究院渔业机械仪器研究所 | Cage culture method, net cage and system including the net cage |
| CN101647413A (en) * | 2008-08-11 | 2010-02-17 | 中国水产科学研究院东海水产研究所 | Method for transforming feeding habits of juvenile fishes of large acipenser sinensis |
| IE20080676A1 (en) * | 2008-08-22 | 2010-03-03 | Anthony Blowick | A self contained and structurally self supporting high density modular aquaculture system |
| CN101836612A (en) * | 2010-05-27 | 2010-09-22 | 中国长江三峡集团公司中华鲟研究所 | B ultrasonic genital gland identifying method of parent fish of Acipenser sinensis |
| CN101836611A (en) * | 2010-05-27 | 2010-09-22 | 中国长江三峡集团公司中华鲟研究所 | Method for regulating gonadogenesis of first-generation parent fish of Acipenser sinensis |
| CN102265805A (en) * | 2011-07-07 | 2011-12-07 | 马宁道 | Stage culture method of finless eel |
| CN102273571A (en) * | 2011-09-21 | 2011-12-14 | 马宁道 | Formula of food habit domestication feed for ricefield eel and food habit domestication method |
| CN103329830A (en) * | 2013-07-12 | 2013-10-02 | 中国水产科学研究院长江水产研究所 | Wild-training method for improving feeding ability of released Acipenser dabryanus |
| CN103461246A (en) * | 2013-09-23 | 2013-12-25 | 中国长江三峡集团公司中华鲟研究所 | Procypris rabaudi fry ecological pond cultivating method |
| CN103583431A (en) * | 2013-11-10 | 2014-02-19 | 华中农业大学 | Method for mainly culturing yellow catfishes and carrying out polyculture on other feeding fishes in pond |
| CN103931958A (en) * | 2014-05-13 | 2014-07-23 | 广东通威饲料有限公司 | Sturgeon fry mouth-opening bait |
| CN104171741A (en) * | 2014-08-24 | 2014-12-03 | 四川省农业科学院水产研究所 | Feed of adult acipenser dabryanus |
| CN104365518A (en) * | 2014-11-27 | 2015-02-25 | 四川省农业科学院水产研究所 | Acipenser dabryanus fry start feeding and domesticating method |
| CN105557595A (en) * | 2016-01-20 | 2016-05-11 | 中国水产科学研究院长江水产研究所 | Cultivation method for brachymystax lenok fry |
| CN106386586A (en) * | 2016-09-09 | 2017-02-15 | 成都市农林科学院 | Indoor large-scale offspring seed cultivation method for Micropterus salmoides |
| CN108668958A (en) * | 2018-04-02 | 2018-10-19 | 武汉中科瑞华生态科技股份有限公司 | A kind of artificial domestication method of mass migration wild fish |
| CN108866203A (en) * | 2018-07-06 | 2018-11-23 | 江汉大学 | A kind of hybrid sturgeon paternity test microsatellite marker primer and its hybrid sturgeon paternity test method |
| CN109182531A (en) * | 2018-07-06 | 2019-01-11 | 江汉大学 | A kind of hybrid sturgeon Individual identification kit and its application |
| CN109480076A (en) * | 2018-11-16 | 2019-03-19 | 湖北清江鲟龙渔业有限公司 | A kind of sturgeon feed preparation method |
| CN110169387A (en) * | 2019-06-05 | 2019-08-27 | 中国长江三峡集团有限公司中华鲟研究所 | A kind of fish-releasing device and application method suitable for a variety of landform |
| CN110169388A (en) * | 2019-06-05 | 2019-08-27 | 中国长江三峡集团有限公司中华鲟研究所 | A kind of flowing water culture pond and application method with water-saving dirt collection function |
| CN115530099A (en) * | 2022-10-27 | 2022-12-30 | 中国水产科学研究院黑龙江水产研究所 | Acipenser parvum female fish and huso male fish hybrid breeding method |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10080336B2 (en) * | 2014-04-16 | 2018-09-25 | Aquatree Global, Llc | Aquaponics system |
| US9873731B2 (en) * | 2014-09-15 | 2018-01-23 | The Lauridsen Group Incorporated | Method for increasing milk production by ruminants |
| US20220338453A1 (en) * | 2021-04-16 | 2022-10-27 | Auburn University | Engineered catfish and uses thereof |
-
2023
- 2023-03-16 CN CN202310276491.1A patent/CN116369250B/en active Active
Patent Citations (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004068941A1 (en) * | 2003-01-27 | 2004-08-19 | Davis Russell P | Integrated system of shellfish production and utilization |
| CN101326899A (en) * | 2007-06-22 | 2008-12-24 | 中国水产科学研究院渔业机械仪器研究所 | Cage culture method, net cage and system including the net cage |
| CN101647413A (en) * | 2008-08-11 | 2010-02-17 | 中国水产科学研究院东海水产研究所 | Method for transforming feeding habits of juvenile fishes of large acipenser sinensis |
| IE20080676A1 (en) * | 2008-08-22 | 2010-03-03 | Anthony Blowick | A self contained and structurally self supporting high density modular aquaculture system |
| CN101836612A (en) * | 2010-05-27 | 2010-09-22 | 中国长江三峡集团公司中华鲟研究所 | B ultrasonic genital gland identifying method of parent fish of Acipenser sinensis |
| CN101836611A (en) * | 2010-05-27 | 2010-09-22 | 中国长江三峡集团公司中华鲟研究所 | Method for regulating gonadogenesis of first-generation parent fish of Acipenser sinensis |
| CN102265805A (en) * | 2011-07-07 | 2011-12-07 | 马宁道 | Stage culture method of finless eel |
| CN102273571A (en) * | 2011-09-21 | 2011-12-14 | 马宁道 | Formula of food habit domestication feed for ricefield eel and food habit domestication method |
| CN103329830A (en) * | 2013-07-12 | 2013-10-02 | 中国水产科学研究院长江水产研究所 | Wild-training method for improving feeding ability of released Acipenser dabryanus |
| CN103461246A (en) * | 2013-09-23 | 2013-12-25 | 中国长江三峡集团公司中华鲟研究所 | Procypris rabaudi fry ecological pond cultivating method |
| CN103583431A (en) * | 2013-11-10 | 2014-02-19 | 华中农业大学 | Method for mainly culturing yellow catfishes and carrying out polyculture on other feeding fishes in pond |
| CN103931958A (en) * | 2014-05-13 | 2014-07-23 | 广东通威饲料有限公司 | Sturgeon fry mouth-opening bait |
| CN104171741A (en) * | 2014-08-24 | 2014-12-03 | 四川省农业科学院水产研究所 | Feed of adult acipenser dabryanus |
| CN104365518A (en) * | 2014-11-27 | 2015-02-25 | 四川省农业科学院水产研究所 | Acipenser dabryanus fry start feeding and domesticating method |
| CN105557595A (en) * | 2016-01-20 | 2016-05-11 | 中国水产科学研究院长江水产研究所 | Cultivation method for brachymystax lenok fry |
| CN106386586A (en) * | 2016-09-09 | 2017-02-15 | 成都市农林科学院 | Indoor large-scale offspring seed cultivation method for Micropterus salmoides |
| CN108668958A (en) * | 2018-04-02 | 2018-10-19 | 武汉中科瑞华生态科技股份有限公司 | A kind of artificial domestication method of mass migration wild fish |
| CN108866203A (en) * | 2018-07-06 | 2018-11-23 | 江汉大学 | A kind of hybrid sturgeon paternity test microsatellite marker primer and its hybrid sturgeon paternity test method |
| CN109182531A (en) * | 2018-07-06 | 2019-01-11 | 江汉大学 | A kind of hybrid sturgeon Individual identification kit and its application |
| CN109480076A (en) * | 2018-11-16 | 2019-03-19 | 湖北清江鲟龙渔业有限公司 | A kind of sturgeon feed preparation method |
| CN110169387A (en) * | 2019-06-05 | 2019-08-27 | 中国长江三峡集团有限公司中华鲟研究所 | A kind of fish-releasing device and application method suitable for a variety of landform |
| CN110169388A (en) * | 2019-06-05 | 2019-08-27 | 中国长江三峡集团有限公司中华鲟研究所 | A kind of flowing water culture pond and application method with water-saving dirt collection function |
| CN115530099A (en) * | 2022-10-27 | 2022-12-30 | 中国水产科学研究院黑龙江水产研究所 | Acipenser parvum female fish and huso male fish hybrid breeding method |
Non-Patent Citations (3)
| Title |
|---|
| 任华 ; 蓝泽桥 ; 孙宏懋 ; 李义勇 ; 兰大华.达氏鲟生物学特性及人工繁殖技术研究.《江西水产科技》.2014,第19-23页. * |
| 李明云编.《水产经济动物增养殖学》.海洋出版社,2011,(第1版),第199-203页. * |
| 陈细华编著.《海洋出版社》.海洋出版社,2017,第30-41页. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN116369250A (en) | 2023-07-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107287245B (en) | Construction method of Glrx1 gene knockout animal model based on CRISPR/Cas9 technology | |
| de Leon et al. | The application of microsatellite markers to breeding programmes in the sea bass, Dicentrarchus labrax | |
| Nielsen et al. | Genetic analysis of common carp (Cyprinus carpio) strains: I: Genetic parameters and heterosis for growth traits and survival | |
| Evans et al. | Loss of genetic variation at microsatellite loci in hatchery produced abalone in Australia (Haliotis rubra) and South Africa (Haliotis midae) | |
| Sekino et al. | Microsatellite-based pedigree tracing in a Japanese flounder Paralichthys olivaceus hatchery strain: implications for hatchery management related to stock enhancement program | |
| Stuart et al. | Replication, integration and stable germ-line transmission of foreign sequences injected into early zebrafish embryos | |
| Calsbeek et al. | Multiple paternity and sperm storage lead to increased genetic diversity in Anolis lizards | |
| CN101911918B (en) | Selective breeding method for disease-resistant superior strains of paralichthys olivaceus | |
| WO2017004936A1 (en) | Breeding method for obtaining heterosis for lined seahorse (hippocampus erectus) | |
| CN106818552B (en) | Method for inducing artificial gynogenesis of siniperca chuatsi by heterologous sperms | |
| Perera et al. | Xenogenesis-production of channel catfish× blue catfish hybrid progeny by fertilization of channel catfish eggs with sperm from triploid channel catfish males with transplanted blue catfish germ cells | |
| Coogan et al. | CRISPR/Cas-9 induced knockout of myostatin gene improves growth and disease resistance in channel catfish (Ictalurus punctatus) | |
| Argue et al. | Growth of channel catfish (Ictalurus punctatus), blue catfish (I. furcatus), and their F1, F2, F3, and F1 reciprocal backcross hybrids in earthen ponds | |
| Bongers et al. | Maternal influence on development of androgenetic clones of common carp, Cyprinus carpio L. | |
| Kato | Breeding studies on red sea bream Pagrus major: mass selection to genome editing | |
| Jerry et al. | Application of DNA parentage analyses for determining relative growth rates of Penaeus japonicus families reared in commercial ponds | |
| CN116369250B (en) | Acipenser dabryanus feeding training method | |
| Schiermeier | Fish farms’ threat to salmon stocks exposed | |
| Kato et al. | Viability, growth and external morphology of meiotic‐and mitotic‐gynogenetic diploids in red sea bream, Pagrus major | |
| CN112680447A (en) | Goldfish gene editing technology and method for creating new goldffish variety by using same | |
| 谷口順彦 et al. | High heterozygosity at Gpi-1 in gynogenetic diploids and triploids of ayu Plecoglossus altivelis. | |
| You et al. | Gynogenesis and sex determination in large-scale loach Paramisgurnus dabryanus (Sauvage) | |
| CN114451335B (en) | Breeding method of ternary hybrid scallop commercial seedlings | |
| AU2016354255B2 (en) | A WW homogametic male decapod crustacean and methods of using the same | |
| CN105746388A (en) | Double-screening and double-inducing technology system for building disease-resistant grass carp pure line |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |