KR101924753B1 - Production mathod of hybrids of fishes and goldfish for fishing - Google Patents

Abstract

The present invention relates to a method for producing a hybrid of Carassius auratus and Cyprinus carpio nudus for fishing. The hybrid production method includes the steps of: (a) preparing multiple water tanks individually configured in accordance with usage conditions in order to produce the hybrid of Carassius auratus and Cyprinus carpio nudus by enabling the fertilization of the eggs of Cyprinus carpio nudus and sperm of Carassius auratus; (b) growing the hybrid fish in the water tanks until the breeding period of the Carassius auratus and Cyprinus carpio nudus starts and selecting only the female Cyprinus carpio nudus and male Carassius auratus exceeding predetermined weight and size standards; (c) simultaneously collecting eggs and sperm of the selected female Cyprinus carpio nudus and male Carassius auratus; (d) mixing the collected eggs and sperm of the selected female Cyprinus carpio nudus and male Carassius auratus by spraying the sperm in order to prepare fertilized eggs; (e) inputting the fertilized eggs in water tanks with artificial reef parts in order to enable the fertilization and hatching of the young of the hybrid; and (f) supplying feed for the swimming young of the hybrid after (e) to grow the same.

Description

[0001] The present invention relates to a production method of hybrids of goldfish for fishing,

The present invention relates to a method for producing a hybrid of a fish for fishing and a goldfish, and more particularly, to a method for producing a new varieties of orange color through the crossing of a goldfish consisting of a fish, The present invention relates to a method for producing a hybrid of a fishing fish and a goldfish which can be utilized as a fishing fish species.

The leather carp is freshwater fish with carp koi. In Germany, artificial improvement of natural carp has been brought to the aquaculture. It is also called a German carp or an Israeli carp. It is the representative fish for fishing in Korea. The water temperature is about 25 degrees and the life span is about 40 years. Spawning occurs in May-June. The ratio of body height to body height is 2: 1, and the growth rate is 2 to 2.5 times faster than carp. The body is similar, but the weight is heavier than the carp. It is cultivated in the same way as native carp. It mainly uses fish meal but does not depend on specific bait.

In addition, goldfish is carp fish with freshwater fish. It is a goldfish modified for ornamental purposes, similar in shape to goldfish, with the same number of chromosomes and the same type. Then, when the fish are separated from the net and repeatedly breed, goldfish are produced and they are variants of goldfish. These goldfish are composed of various species such as goldfish, goldfish, comet, oranda, pearl lint,

Here, the goldfish is called a Japanese goldfish and is a common goldfish-shaped goldfish. It is the first species to be improved from a red goldfish. Its tail fin is bifurcated or quadrupled. In addition, Comet is a varieties of mutations found in the nets of the Washington Fisheries Commission of the United States of America, which were flown from Japan, and immobilized with goldfish. Comet is named after comet, as if swinging around in a swollen tail. It is characterized by the formation of long multiple fins.

Hybridization, on the other hand, is a technique to produce new species for growth, disease resistance, meat quality, and environmental adaptation, which are two economically important traits of crossing between two species with different genotypes and phenotypes.

In particular, interspecific hybridization has a high probability of obtaining a hybrid stress through a combination of desired traits, and various attempts have been made (Korean Patent Publication No. 10-1999-0045938, Korean Patent Registration No. 10-0844339, 10-2012-0083990).

Korean Patent Publication No. 10-1999-0045938 Korean Patent No. 10-0844339 Korean Patent Publication No. 10-2012-0083990

In order to solve such problems, the present invention was devised by the above-mentioned background arts, and it is an object of the present invention to provide a method for producing new varieties of orange series through the crossing of the sea cucumber and goldfish, And to provide a method for producing a hybrid of a fishing fish and a goldfish that can be utilized.

It is another object of the present invention to provide a method for producing a cross between a fish for a fish and a goldfish, in which crossing is carried out in a nature-friendly manner without using a separate hormone.

It should be understood, however, that the scope of the present invention is not limited thereto, but includes any object or effect which can be understood from the solution or the embodiment of the present invention without explicitly mentioning it.

According to an embodiment of the present invention, there is provided a method of preparing a fish tank, comprising the steps of: (a) preparing a plurality of water tanks individually prepared according to use conditions to produce a crossbred fish, step; (b) breeding in the water tank until the time of scattering of the fish and the goldfish arrives, and selecting only male and female fishes whose size and weight exceed a certain standard; (c) simultaneously collecting eggs and semen from the selected female facial and male goldfish, respectively; (d) injecting the goldfish's semen into the egg of the fish so as to form an embryo; (e) injecting the embryo into a water tank provided with an artificial fish candle so as to embryo the embryo and hatch the embryo; And (f) after the step (e), growing the frog fishes swimming while feeding the feed.

According to an embodiment of the present invention, in the step (a), the spermatozoa and the spermatozoa to be sampled are separated and the goldfishes are separated from each other, and four days before the fermentation and the goldfish are reared, And the temperature of the water is maintained at 18 to 22 ° C for 4 days, and a water tank for feeding the embryo, and the temperature of the ground water is kept at 22 ° C to 24 ° C so that the embryo can be implanted and hatched And the spermatozoa collected from the fishes and the goldfish are adjusted so that the spermatozoa collected from the fishes and the goldfishes are collected, 30% by weight of distilled water, and 70% by weight to 85% by weight of distilled water.

According to an embodiment of the present invention, in step (c), the egg of the fish is washed with physiological saline and collected by abdominal compression method, and the semen of the goldfish is used to collect the goldfish After anesthetizing, the fish is characterized by collecting from the genitalia of a male goldfish using a syringe.

According to an embodiment of the present invention, the step (d) further comprises the step of separating an unfixed egg and a semen from the embryo.

According to an embodiment of the present invention, the pellet feed is characterized in that the pellet feed comprises a syrup mixed with 15g to 20g of boiled egg yolk crushed per 100g of powdered milk of a newborn child 100 days old.

According to the embodiment of the present invention, new varieties can be produced through crossing between the sea bream and the goldfish, and the new varieties can be utilized as fishing fish species and cultured fish species, thereby creating economic power for the fishing industry There is an effect that can be.

In addition, the various and advantageous advantages and effects of the present invention are not limited to the above description, and can be more easily understood in the course of describing a specific embodiment of the present invention.

FIG. 1 is a flowchart illustrating a process of hybridizing a fish with a goldfish according to an embodiment of the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. In the drawings, the same reference numerals are used to designate the same or similar components throughout the drawings. In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear.

In describing the components of the present invention, terms such as first, second, A, B, (a), and (b) may be used. These terms are intended to distinguish the constituent elements from other constituent elements, and the terms do not limit the nature, order or order of the constituent elements. When a component is described as being "connected", "coupled", or "connected" to another component, the component may be directly connected to or connected to the other component, It should be understood that an element may be "connected," "coupled," or "connected."

The method of producing a hybrid of a fish of the present invention and a goldfish is an invention capable of producing a new variety through hybridization of a fish with a fish and goldfish and utilizing the fish as a fish species and aquaculture for a new variety produced. As described above, the step of improving the ground water temperature and water quality (S110), sorting and separating the korean / goldfish (S120), collecting sperm from the fish eggs and goldfish (S130), adjusting the fertilization step (S140) S150), and a breeding step (S160).

In step S110 of improving the ground water temperature and water quality, a water tank for raising a sole and a goldfish to produce a hybrid of a sea fish and a goldfish, a conching and hatching tank for conceiving and hatching, The spermatozoa of the spermatozoa collected from a goldfish consisting of eggplant, corn, or comet is filled with water according to the use condition of each group.

In this case, in the case of the water tank for the breeding tank, the ground water was previously received 4 days before the rearing of the fish and goldfish, and the water temperature was maintained at 18 to 22 ° C for 4 days, Goldfish provide the optimal environment to live until ovulation.

In addition, the embryo and hatching water tanks are provided with fertilization eggs in which fertilized eggs are fertilized in a crystal water tank and spermatozoa of spermatozoa of goldfish are put into the fertilization water tank, and the fertilized eggs are managed until hatching is performed. And the clean groundwater is introduced before picking eggs from the ovary where the ovulation is carried out. The groundwater is supplied at a temperature of 22 ° C to 24 ° C so that implantation and hatching can be performed.

At this time, the conception and hatching water tanks may be composed of a plurality of hatching tanks, and one of the plurality of hatching and hatching water tanks is formed with artificial fish shafts made of polypropylene so that the hatching hybrids can be raised .

In addition, a fertilization water tank is used to fertilize the sperm egg and the sperm of the goldfish, and a mixture of a certain amount of distilled water and physiological saline is injected.

Here, the mixed liquid composed of the distilled water and the physiological saline is mixed in a total weight of 15% by weight to 30% by weight of physiological saline and 70% by weight to 85% by weight of distilled water. Preferably, 25% by weight of physiological saline and 75% by weight of distilled water are mixed.

The step of improving the ground water temperature and water quality (S110) is to make hybrids without adding any artificial medicines by using sperm of egg and goldfish.

Accordingly, it is possible to produce hybrids between a fish and a goldfish without using a medicine such as a scattering-inducing agent which is not marketed in the past as in the past.

In step S120, the size and weight of female fishes and male goldfishes fed from a farm or a fishing ground are measured, and only female fishes and male goldfishes exceeding a predetermined standard are selected and when the spawning season arrives And the breeding is carried out in a tank for breeding.

In this step S120, the female fishes weighing 3 kg to 5 kg and the male goldfishes weighing 20 g to 200 g are individually separated to allow breeding until the spawning season arrives.

The sperm retrieval step (S130) of fetal oocytes and goldfishes is a step of collecting oocytes and semen, respectively, when the female fetus is reared in the breeding tank and the male goldfish arrives at the spawn time.

The sperm retrieval step (S130) of the oocyte and the goldfish is such that only the female fry that can be scattered among the female fry during the breeding is moved to a separate crystal water tank and then the oocyte is collected.

At this time, it is preferable that the oocyte is collected by abdominal compression after washing with physiological saline, and the oocyte collected is directly supplied to the crystal water tank.

In addition, among male goldfish in breeding, only male goldfish that can spawn are moved to a separate crystal water tank, and semen is collected.

In this case, when the semen is sampled, the sample is washed with physiological saline and then anesthesia is performed. When the anesthesia is confirmed, semen is sampled from the genitalia of the male goldfish using a syringe, So that the input is made by the inserted crystal water tank.

At this time, it is preferable that the semen of the female fetus and the male goldfish are sampled at the same time. This is to prevent sperm liquefaction and to maintain the motility of semen in sperm as much as possible.

In the modification step (S140), the fertilized eggs of the sperm and the sperm contained in the semen of the goldfish, respectively, are supplied to the fertilizer tank. After the eggs of the sampled female fish are put into a crystal water tank, The eggs are evenly sprinkled on the egg.

At this time, the semen collected from the male goldfish can be diluted with physiological saline to separate the impurities and spermatozoa, and only the separated spermatozoa can be evenly distributed to the female egg.

In addition, the artificial fish made of polypropylene is placed in the crystal water tank in which the fertilized oocyte and the goldfish are mixed, and the artificial fish is left for 12 hours and 24 hours so as to be corrected around the artificial fish.

Thereafter, the fertilized eggs, which have been fertilized in the fertilization tank, are transferred to the fertilization and incubation tank, and the fertilized eggs and semen can be separated from fertilized eggs.

If the fertilized oocytes and sperm are separated from fertilized eggs, it is preferable to carry them before transferring to fertilization and incubation tanks. Only fertilized fertilized eggs should be transferred to fertilization and incubation tanks.

At this time, it was judged that the fertilized eggs were fertilized in the fertilized water tank after the fertilization was induced, and those that were sedimented to the bottom of the fertilized water tank were judged not fertilized, .

In the conception and hatching step (S150), the embryo is fertilized and the embryo is hatched. The embryo is inserted into the area where the artificial fish is placed, and the embryo is implanted for 4 to 5 days The temperature in the conception and incubation tanks is kept constant.

Then, when the embryo is implanted and the orange-flavored carnivorous hatching hatches, the hatchlings are incubated until they are able to swim in the conception and hatching tanks. At this time, the swimming time of the incense fish is 3 days, and when the fish is over 3 days, the incense fish are moved to the conception and hatching tanks for rearing.

The step of raising the poultry (S160) is a step of raising the incubated fish in the incubation tanks and the incubation tanks for breeding until the incubated rabbits are incubated.

Here, the fry feed can be a mixture of powdered milk and egg yolk.

At this time, it is preferable to use only powdered milk of infant that is 100 days old and eat egg powder, and egg yolk should be only crushed after removing the whites from boiled eggs.

In addition, the mixture may be prepared by mixing 15 g to 20 g of egg yolk with 100 g of powdered milk, but is not limited thereto.

In addition, the fry feed was fed three times a day for 3 to 5 days immediately after the fermented fish was transferred to a conception and hatching tank for rearing, and 3 times a day after 5 days, It can be supplied with but not limited to poultry feed.

Also, it is preferable that the breeding is carried out while the temperature of the breeding tank is maintained at a temperature of 18 to 24 ° C at the time of breeding the breeding, but it is not limited thereto, and after the feeding date of the breeding feed exceeds 5 days, In other words, starting from the 6th day, it can be made to grow in a nature-friendly manner outdoors.

It is to be understood that the terms "comprises", "comprising", or "having" as used in the foregoing description mean that the constituent element can be implanted unless specifically stated to the contrary, And all terms including technical or scientific terms are to be construed as further including other elements unless the context clearly dictates otherwise as generally understood by one of ordinary skill in the art to which this invention belongs. Have the same meaning.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or essential characteristics thereof. . Therefore, the embodiments disclosed in the present invention are intended to illustrate rather than limit the scope of the present invention, and the scope of the technical idea of the present invention is not limited by these embodiments. The scope of protection of the present invention should be construed according to the following claims, and all technical ideas within the scope of equivalents should be construed as falling within the scope of the present invention.

Claims (5)

(a) The spermatozoa of the goldfish consisting of the ovary of the sea urchin, the sea bream or the comet are modified so as to breed the female fishes and the male goldfishes to be separated from each other to collect the eggs and sperm to produce the crossbred fishes And the embryos between the male and female goldfishes are fed into the water tank for breeding to maintain the water temperature at 18 to 22 ° C for 4 days before the watering of the fishes and goldfishes is carried out four days before, The temperature of the groundwater is maintained at 22 ° C to 24 ° C so that conception and hatching can be carried out and the breeding is carried out until the time when the hatchlings of the hatching fish are swimming, The total weight of the water tank, the sperm sampled from the fish, and the sperm collected from the goldfish, Preparing a modified tank that is a mixture of a mixture consisting of salt water is added 15% by weight to 30% by weight, distilled water 70% by weight to 85% by weight;
(b) selecting female fishes weighing from 3 Kg to 5 Kg and male fishes having a body weight of 20 g to 200 g, separately and separately, and raising in the breeding tanks until the time of scattering is reached;
(c) washing only the female facial hair that can be scattered among the female facial being raised by the step (b) with physiological saline, collecting the oocyte by the abdominal compression method, putting it into the crystal water tank, Anspruch [en] Anesthesia of an available male goldfish, collecting sperm from the genitalia of the male goldfish using a syringe and injecting it into a separate crystal water tank, simultaneously collecting the oocyte and semen;
(d) diluting the semen collected in the step (c) with physiological saline to separate impurities and spermatozoa, and then spraying the separated spermatozoa to the fertilized water tank into which the egg is put, Leaving for 12 to 24 hours to form fertilized eggs by fertilization;
(e) After the step (d), it is judged that the correction of the floating in the correction water tank has been made, and the ones having settled down to the lower part of the correction water tank, And separating the sperm from the embryo and injecting the embryo formed in the embryo and hatching tank with the artificial fish into the embryo and hatching the embryo of the orange series; And
(f) After the step (e), immediately after moving the frying pellets comprising the mixture of 15g to 20g of boiled egg yolk crushed per 100g of powdered milk to the conching and hatching tanks, The liver is supplied three times a day, and after 5 days, three times a day are supplied, and growing outdoors from the sixth day;
The method of producing a hybrid of a fish for fishing and a goldfish.
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