KR20180047631A

KR20180047631A - Controlling method of the time of sea cucumber artificial seed production through management of parents sea cucumbers in land farm

Info

Publication number: KR20180047631A
Application number: KR1020160144147A
Authority: KR
Inventors: 이재두
Original assignee: 이재두
Priority date: 2016-11-01
Filing date: 2016-11-01
Publication date: 2018-05-10

Abstract

According to the present invention, an early sea cucumber production method comprises: (a) a step of lowering a breeding water temperature of adult sea cucumbers to 8°C for 12 weeks and the raising the breeding water temperature to 18°C for 14 weeks to manage the breeding water temperature; (b) a step of allowing the adult sea cucumbers in step (a) to spawn and release sperm by spawn stimulation using water temperature stimulation, surface stimulation, and dry stimulation; (c) a step of developing fertilized eggs obtained in step (b) in a water tank; (d) a step of raising baby sea cucumbers developed in step (c) on a corrugated sheet until the baby sea cucumbers grow to 0.5 g; and (e) a step of raising the baby sea cucumbers on a special mesh sheet when the baby sea cucumbers grow to 0.5 g in step (d). Superior sea cucumber seeds are stably secured, and a period during which sea cucumber seeds can be produced is extended. The farming period of sea cucumbers can be shortened to contribute to an increase in income of sea cucumber farms.

Description

TECHNICAL FIELD [0001] The present invention relates to a method for regulating the production period of sea cucumber artificial seedlings using a method for controlling the production period of sea cucumber artificial seeds

The present invention relates to a method for regulating the production time of a seedling through the management of a sea cucumber. More particularly, the present invention relates to a method for controlling the production time of a seed cucumber, Controlling the production of embryos and growing them into ginseng.

Sea cucumbers are classified as Phylum Echinodermate and belong to the class Holothuroidea. They are named as sea cucumbers because their efficacy is the same as that of ginseng. It is recorded in the ancient documents such as Antarctic fish, sea cucumbers, poultry, sea horses, and black bears, and in Japan, it is called nakara in the sense that it is like a rat as a nocturnal fish.

The shape of the body is a long cylindrical shape back and forth, and there are several lumps on the back. The front end of the body has a mouth open, several tentacles around it, and an anus at the back end. Most species also have a lot of tunnels with thin tubes on the underside, which crawls under the sea. A species with no legacy floats in the sea or buries in sandy mud. In the skin there are small fragments of calcareous bone fragments. There is a distinction between male and female, but it is difficult to distinguish by appearance.

Sea cucumbers repeat their lives every year according to changes in water temperature and season. At around 8 ~ 16 ℃, the appetite is active and the exercise is active. At 17 ~ 19 ℃, the exercise is not active, the appetite decreases and the digestive tract becomes ineffective. When the temperature exceeds 25 ℃, sleep. When eating food, tentacles around the mouth put mud or sand under the sea into the mouth to feed organic sediments such as seaweed, and the sand and excreta are sent out. When stimulated from the outside, the intestines are cut off and sent out of the anus, which is regenerated and regenerated.

The sea cucumber is rich in vitamins and minerals, so it relieves fatigue and boosts energy, restores the function of the appetite or tired body, smoothes the metabolism and encourages the child, the elderly, pregnant women It is known to have an effect of restoring. In addition, Holotheen in sea cucumber destroys bacteria, prevents blood from clotting, has anti-cancer effect, and fatty acids in sea cucumbers are effective for asthma, colon cancer and arthritis. The sea cucumbers are also considered as the best tonic medicine because they cleanse the blood and strengthen men's sexual function.

Since 1990, the National Fisheries Research and Development Institute has succeeded in producing sea cucumber artificial seeds for the first time in Korea, and the sea cucumber culture has been actively developed. From 2006, the development of the sperm culture, the festival culture and the aquaculture culture industrialization technology has been promoted.

Conventional production of artificial seeds of sea cucumbers is based on the method of inducing spawning and fertilization through spawning stimulation to naturally harvested sea cucumbers, obtaining seedlings by obtaining embryos, and the production period of artificial seedlings is from the end of May It was limited to July. In addition, there is a difficulty in developing a good seedling based on the selection of good traits while harvesting the natural ginseng that are necessary for artificial seed production year by year.

Therefore, in the present invention, by securing a good quality fresh ginseng, increasing the initial cultivation period of the sea cucumber seedlings and changing the necessary aquaculture device according to the growth of ginseng, the selection and management of the ginseng for the production of artificial seeds and the management of the ginseng It provides a method of controlling the production time of sea cucumber seedling seedlings so that the artificial seedling of sea cucumber can be produced early to early March before the natural spawning season.

Korean Patent No. 10-1397758 discloses a soybean meal comprising 20% by weight of fish meal, 20% by weight of wheat flour, 10% by weight of seaweed powder and 10% by weight of insect worms, 20% by weight of soybean meal or 20% by weight of fermented soybean meal, When the soybean meal is selected, 10% by weight of makgeolli is prepared, and the remaining composition is 100% by weight of 10 to 20% by weight of the mud powder. Korean Patent Laid-Open Publication No. 10-2012-0059718 discloses an artificial reed including a fishing net having a certain space floated in water and an artificial reed which is installed at a certain interval so as to stand in the water in the fishing net, Discloses an apparatus and a method for producing a sea cucumber seedling in a festival farm having an effect of easily securing a production space of a sea cucumber seedling growing in place of a land nursery that is expensive in facility cost and reducing production cost. In Korean Patent No. 10-1349056, a support member 10 fixed at an angle to a bottom of a farm or vertically; An upper plate 20 and a lower plate 30 rotatably provided on the upper and lower sides of the support member 10; A plurality of attachment substrates (40) positioned between the top plate and the bottom plate, one end of which is fixed to the support member (10); First connecting means (50) connecting the upper plate, the lower plate and the attachment substrate to each other so as to maintain a constant gap therebetween; And a second connection means (60) for connecting one end of the upper plate (20) and an upper end of the support member (10) to each other, and a method for manufacturing a sea cucumber seed production method . Korean Patent Laid-Open Publication No. 10-2012-0063618 discloses a first step of draining seawater containing red sea gins, a second step of drying red seaweed in an inverted state for a first predetermined time, And a third step of stimulating red sea gins in the water tank, and a fourth step of blocking light irradiated to the red sea gull for a second predetermined period of time. However, in the present invention, the present invention relates to a method for producing early seedling of the sea cucumber according to the present invention, which manages water temperature of breeding ginseng to induce early scattering, and transferring the embryo obtained in the water tank to a specific growth period, The method differs in its composition and effect.

The production of artificial seeds of sea cucumbers has been restricted from late May to July, because cultivars for the production of conventional sea cucumber artificial seeds were only supplied to natural harvesting season in accordance with the seedling production period, . It is an object of the present invention to provide a method for producing artificial seedling of sea cucumber early through the management and scattering of natural ginseng for the production of sea cucumber artificial seeds.

In order to solve the above-mentioned problems, the present invention provides a method of controlling the temperature of raising water of the ginseng to 8 캜 for 12 weeks and then raising the temperature to 18 캜 for 14 weeks, (a) (B), (c) generating the embryos obtained in step (b) in a water tank, (c) growing the ginseng in step (c) And (d) raising ginseng in a special mange when the ginseng is grown to 0.5 g in the step (d) and (d), thereby providing an early seedling production method of sea cucumber.

By using the method of producing early seedling of sea cucumber according to the present invention, it is possible to produce a superior artificial seedling of sea cucumber earlier than in the past, thereby shortening the cultivation period of sea cucumber to reduce costs and secure a good quality sea cucumber seedlings, The period in which seed production can be extended can contribute to the increase in income of the sea cucumber cultured fish.

Fig. 1 is a photograph showing the appearance of sea cucumber to be used as a raw ginseng.
FIG. 2 is a graph showing the water temperature management from fresh ginseng to fresh ginseng in the management method of the present invention, or to scattering in the next year.
Fig. 3 is a photograph showing an automatic temperature control facility required for water temperature management of a ginseng.
FIG. 4 is a photograph showing a scattering period management method in the method of managing fresh tomato according to the present invention.
FIG. 5 is a photograph showing a self-produced dry solid feed for the purpose of controlling the feed of the ginseng.
FIG. 6 is a photograph showing the production process of artificial seedlings produced by using the ginsengs managed by the method of the present invention.
FIG. 7 is a micrograph showing the embryo and hatching process obtained from the ginseng, which is managed according to the method of producing the sea cucumber early seed of the present invention. A represents embryo, B represents 2-16 years, C represents cysts, and D represents auroral.
FIG. 8 is a photograph showing that the sea cucumber larvae produced by the method of producing the sea cucumber early seed according to the present invention are in good condition.
FIG. 9 is a photograph showing the development management of ginseng according to the method of producing sea cucumber early seed according to the present invention.
Fig. 10 is a confirmation of the production of seedling of seedling produced through the control of the ginseng according to the present invention.

1. Conventional method

Maternal sea cucumber for artificial seedling is collected before and after the seedling production and is discharged for 3 ~ 4 days completely. After the excreta is completely discharged, the eggs are scattered and fixed by the method of head stimulation, water temperature stimulation, And the fertilized eggs are transferred to the incubation tank for hatching and growing.

The embryo hatches and undergoes the larval stage, where the water temperature is maintained at 22 ~ 24 ℃ and feeds on phytoplankton or concentrated food. The sea cucumbers grow through the larval stages of auricularia, doriolaria and pentactula, and grow on the waters of the pentactula larvae. After a few months, Growth.

Conventional sea cucumber artificial seed production uses naturally harvested sea cucumbers as raw eggs, and eggs harvested by natural harvesting are spawning irritation, which causes irregularities and irregularities at once regardless of maturity of eggs and sperm in the gonads Therefore, the fertilization rate is not as high as that of egg production. In addition, the number of fertilized eggs that can be obtained per individual population is limited because spontaneous harvesting is a single spawning and the spawning is completed.

In the past 1 to 7 days after harvesting with natural harvested natural ginseng, spawning and fertilization are induced by spawning stimulation, and spawning and fertilization are discarded, so that the next year for artificial seed production will depend on natural harvest The production period of the seedling is limited to natural habitat from late May to July, when the sea cucumber spawns and hatches and grows. Therefore, there is a problem that the survival rate of the farm is reduced when the seedling is stocked at this time and if it is cultured, it has three periods of lowering until it is shipped.

The present invention relates to a method for preparing artificial seedlings of sea cucumbers by increasing the initial cultivation period of sea cucumber seeds and changing the necessary cultivation methods according to the growth of ginseng seeds, We have established an early seedling production method of sea cucumber which can be produced early in the month. Hereinafter, the present invention will be described in detail with reference to specific examples.

2. Screening

Mothers managed for artificial seed production are first collected from nature and secured. In general, sea cucumbers are naturally hatched from late May to July, so the sea cucumbers used as forage cultivars are harvested by daughters between March and June. The selected moths were purchased from Baekryungdo and Chungnam Rhododendrons.

2.1 Screening by Outline

Fig. 1 is a photograph showing the appearance of sea cucumber to be used as a raw ginseng. Among the sea cucumbers collected from natural seawater, the surface is smooth and unscratched, the projections are large and aligned, the color is clear and the weight is more than 150 g per person.

2.2 On the feeding activity Selection by

Momam, who was naturally harvested, was allowed to fast for one week after the water tank was settled. During this fasting period, the feeding is stopped and the sea cucumbers emptied the guts by returning the filtered seawater. After that, the feed was fed at 3% of the weight of the sea cucumber.

2.3 Feed feed Selection by

Conventional sea cucumber feed is a form in which pearl is mixed in a powdery feed and sprayed on a water tank, and the sea cucumber is crawled while it is stacked on the bottom of the water tank. Therefore, it is difficult to confirm the amount of the food per sea cucumber, There was a difficulty. In the selection step of the fresh ginseng and the method of producing early sea cucumber seeds according to the present invention, a piece-shaped solid feedstuff is used in order to grasp the level of feeding activity at the time of feeding and to use the activity activity to select the ginseng. When the feed is fed to the bottom of the water tank, the sea cucumber on the bottom or the wall collects in feed for the feeding. At this time, the low activity sea cucumber is removed from the moths group, Only high individuals can be selected. In addition, since the slice solid feed can not be easily released in the feeding water, it is possible to feed the slice to the upper part of each animal, and it is easy to grasp the amount of each individual to feed, so the amount and continuous observation of the slice solid food Which is a high activity level.

3. Fox Controlling the timing of artificial seed production through land management

In the past, there was a problem that there was no preservability, such as natural disintegration after being collected. Therefore, since the individual weight of the sea cucumber used as the raw ginseng is also covered only by natural harvesting, more than 150 g is used. However, since the annual weight of the sea cucumber is 250 ~ 400g, the amount of eggs is increased and the efficiency of harvesting is increased.

The selected sea cucumbers after the natural harvest are used as a raw material for sea cucumber artificial seed production through management of water temperature, feed and water quality throughout the year, Among these, water temperature management is the most important for the management of the beans.

Sea cucumbers naturally spawn once in early May to July, and then in winter, when the water temperature increases in summer, they can spend the rest of the year and spawn again. It is a technical feature of the present invention to quickly regain the spawning ability of the sea cucumber through a certain change in water temperature while controlling the water temperature corresponding to the natural environment of this seasonal temperature change.

3.1 Low-temperature period management

FIG. 2 is a graph showing the water temperature management from the fresh ginseng collection or the fresh ginseng to the next year scattering in the method of producing the early ginseng seed according to the present invention. The water temperatures of sea cucumbers from A to C were shown in time order. From June 1 to December 5, shown in blue, it is managed to correspond to natural water temperature. In this period, the sea cucumbers are gradually exposed to low temperature environment since the water temperature is gradually lowered from summer, so the water temperature of the rearing water is slowly lowered to create an environment that passes through the winter after spawning. Filtration and sterilized breeding seawater are adjusted to have the appropriate dissolved oxygen, pH and salinity, and heated and cooled to the appropriate temperature through the heater and supplied.

(2) lowering to 10.3 캜 for 4 weeks from the step (1), lowering the water temperature to 8 캜 for 4 weeks from the step (2) ) To lower the water temperature by 8 ° C step by step. In normal winter sea cucumber culture, 20 to 25 ° C can be maintained in general seawater or lower temperature in natural environment. However, if the temperature is raised from 8 ° C to the spawning induction through the preliminary experiment of the present invention, Respectively.

FIG. 3 shows an automatic temperature control facility required for water temperature management of the freshwater ginseng. The water temperature of the breeding water is intended to advance the spawning cycle of natural ginseng more than the natural state, and it is important to raise the temperature of the water accurately because it includes the process of warming the water temperature despite the natural seasonal change. Therefore, it adopts automatic thermostat to precisely control the water temperature, and the temperature is lowered or increased within a certain period of time.

3.2 Pre-egg management

The general sea cucumber spawning is performed from late May to July, but the production of artificial seeds of sea cucumber using the method of the present invention is advantageous in early seed production. In the low-temperature maintenance period, the temperature of the raising water is lowered to 8 캜, and then the temperature is raised to 18 캜 before scattering induction.

As shown in FIGS. 2 (B) and 2 (C), the water temperature is raised at a temperature of 8 캜 for raising the temperature by 0.5 캜 every week for 3 weeks and then raised to 9.5 캜 for 3 weeks. (5) raising the temperature to 17.5 ° C to 18 ° C for 8 weeks, and (6) maintaining the temperature at 18 ° C from the step (5) to the induction of scattering.

During this period, sea cucumbers prepare for maturity in preparation for the coming of the spawning season, regardless of the natural season, by changing the temperature of the breeding water.

3.3 Spawning Time Management

FIG. 3 is a photograph showing a scattering period management method in the method of managing fresh eggs of the present invention. The sea cucumbers managed during the pre-spawning period can produce fertilized eggs by scattering and spinning through spawning stimulation. Spawning stimulation is performed by general overproduction, surface stimulation, and 5 ° C water temperature stimulation. Exhaust stimulation is done by draining the entire amount of seawater in the rearing tank and exposing it to room temperature for 30 to 40 minutes. Thereafter, the surface of the ginseng was sprayed with surface water to induce surface irritation, and water was added to the water tank at 22 to 23 ° C, which was 5 ° C higher than the control temperature for the spawning period of 18 ° C.

Spawning of a common sea cucumber occurs once a year, and eggs and sperm of the gonad are released regardless of maturity by spawning stimulation. The egg of the sea cucumber, which is easy to fertilize due to its maturity, is characterized by a transparent and smooth surface. However, the immature eggs have a characteristic of being white and uncooled. In the case of scattering through the management method of the fresh ginseng according to the present invention, only a part of the eggs are released by the spawning stimulation, and the maturity of the spawning eggs is high, so that the fertilization rate is much higher than that of the natural ginseng by natural harvesting.

Once the spawning is over, the spawning time is again 7 ~ 10 days in a temperature environment of 18 ℃, and then the spawning is repeated several times by repeated spawning stimulation. Through this experiment, we could observe the results of this study. In this way, one of the larvae spawned enough mature eggs up to 5 times,

Therefore, while the amount of eggs produced by general natural harvesting spawals ranged from 2 to 3 million eggs per egg, the eggs managed by the method of the present invention were scattered in an amount of 2 to 5 million eggs per egg, It was found that the flood efficiency was more than 160% than the conventional method. Also, it was confirmed that the high fertilization rate is favorable for mass production of good seedling.

According to the method for managing fresh ginseng according to the present invention, the period of production of sea cucumber seedlings can be extended over the period of the conventional method. In other words, the production of artificial seeds of sea cucumber from naturally-picked ginseng can be obtained from the end of May to July and the seedling can be released after November. However, Can be used to obtain embryos from the March to September of the sea cucumber at the desired time, and the seeds can be released from August to November.

According to the present invention, the seedling of 3 ~ 5 cm in length and 2 ~ 5 g in weight is produced in a large quantity .

After spawning about 5 times a year, the water is transferred to a natural water tank and managed according to natural water temperature without heating or cooling separately. After that, water temperature management will start from October or November according to the artificial seed production plan for the next year.

3.4 Feed management

FIG. 5 is a photograph showing a self-produced dry solid feed for the purpose of controlling the feed of the ginseng. Conventionally, for the production of artificial seeds of sea cucumbers, the seedling was used for natural harvesting, and after the harvesting, the seedless sea cucumber was not preserved. Therefore, there was no food management method for the preservation and management of. Therefore, the present inventors made and supplied a feed for general ginseng for gonadal growth and a feed for egg production for artificial seed production for supplementing spawning season protein, in comparison with a general sea cow farm designed for weight gain.

3.4.1 Feeding of common mothers

6% by weight of protein feed (abalone powder, scallop, pearl mash meat powder), 4% by weight of sea tangle, 4% by weight of seaweed, 3% by weight of seaweed, 2% by weight of auditory powder, 20% by weight of diatomaceous earth, 50% by weight of fine surface pearl on the west coast containing diatomaceous earth were mixed and kneaded for 12 hours for cutting to 5 ㅧ 7 cm. The protein content was 20% or more, and the nutrients were fortified with additives such as spirulina, appetite inducing yeast, digestive agent, and bithimine, and were fed at 3 to 6% of body weight twice a day.

3.4.2 Feeding of eggplant for spawning season

In the spawning season, sea cucumbers provide protein and nutrients more than other times to spawn healthy eggs in large quantities. Many preliminary experiments have been carried out to observe that 15 days before the spawning by spawning stimuli, a protein - supplemented spawner diet should be supplied for energy supply for maturity and spawning.

As a result of observing the behavior and feeding activity of the sea cucumber continuously during the pre-spawning period and comparing the gonads periodically, the behavior of the sea cucumbers showed that when the sea cucumber shakes its head on the water barrier, the water temperature is lowered and the protein is reinforced It is preferable to prepare feedstuffs of egg yolks for egg yolks and to change egg yolk feedstuffs that have been protein-augmented at the temperature of 16 ~ 18 ℃ in step (5) .

The egg yolks of boiled eggs were mixed with the whole feed mixture and egg yolk at a weight ratio of 1: 0.2. 50% of the surface pearl on the west coast containing mite, mackerel, kelp, seaweed, hearing powder, protein feed (abalone powder, scallop, mulberry meat powder), cultivated feed, Yeast, liver yeast, and marine red yeast were added and dried for 12 hours.

The protein content was 20% or more, and the nutrients were fortified with additives such as spirulina, appetite inducing yeast, digestive agent, and bithimine, and were fed at 3 to 6% of body weight twice a day.

4. Early seedling production of sea cucumber

4.1 Separation and Tiling of Embryos

FIG. 6 is a photograph showing the production process of artificial seedlings produced by using the ginsengs managed by the method of the present invention. The fertilized eggs that have been fertilized by spawning and spinning are collected in a net and separated, and then fertilized with fertilized eggs. From the next day after fertilization, fertilize the seawater daily to ensure that fresh breeding stock is continuously supplied.

FIG. 7 is a micrograph showing embryo and hatching process obtained according to the method of the present invention for producing sea cucumber early seed. A is a fertilized egg, B is a 2 to 16 year old abortion, C is a cystic exhaust, and D is a auriolaria state. In one experimental example of the present invention, embryos were obtained at least two months earlier than that in the production of common sea cucumber seedlings, and most of the produced embryos were generated as shown in FIG. This suggests that mature eggs are scattered about five times in comparison with the production of ordinary sea cucumber seedlings which are scattered once a year, so that the rate of fertilization is higher than that of egg production, and most of the fertilized eggs are grown as ginseng.

FIG. 8 is a photograph showing that the sea cucumber larvae produced by the method of producing the sea cucumber early seed according to the present invention are in good condition. The best way to identify the health of sea cucumbers in the sea cucumber culture is to observe the status of the sea cucumber, intestines and healthy eating activity.

4.2 Management of ginseng development

FIG. 9 is a photograph showing the development management of ginseng according to the method of producing sea cucumber early seed according to the present invention. By changing the necessary aquaculture equipment according to the growth of ginseng, it is possible to secure a stable sea cucumber seedlings.

The ginseng grown through the larval stage is adhered to the wafers and the initial wafers are managed by laying them horizontally on the bottom of the aquarium. At this time, small ginseng is easy to move to food. After about one month after hatching, the papan is placed vertically on the bottom of the aquarium to increase the movement of the sea cucumber. Through this process, the muscles of the protrusions of the sea cucumbers can develop and form a good shape, thus increasing the size and weight of the sea cucumber.

When the ginseng reaches about 0.5 g, it moves the ginseng from the papan to the special pusan. Special nets are made of several layers of nylon mesh and are seated when feeds are fed, and the sea cucumbers are attached to the nets to facilitate feeding. In addition, because it is easy to grasp the feeding activity and bowel activity status of the ginseng, it is possible to select more easily according to the intestinal health condition of the ginseng.

5. Comparison of common sea cucumber artificial seed with the sea cucumber early seed of the present invention

According to the control method of the sea cucumber artificial seed production time through the management of the sea cucumber of the present invention, the comparison between the artificial seeds produced by the management cucumbers produced by 2013 and the artificial seeds of the sea cucumbers produced by the one- As follows.

In the case of ordinary seedling, the weight of one seedling was 0.5 ~ 2g at the time of feeding, whereas the seedling produced by the same method of the present invention, which was supplied at the same time, was 2 ~ 5g in weight and larger in weight and size. The difference in size of seedlings affects the culture period and survival rate of sea cucumber.

In the case of the seedling produced by the management of the present invention, the cultivation period was shortened by 6 to 12 months by 16 to 30 months, while that of the ordinary seedling was increased by 36 months or more. In the case of souring, the cultivation period could be shortened from 24 to 30 months for the existing 36 months, and from 16 to 24 months for the festival type.

Through shortening of the cultivation period, by shortening the number of times that one to two times of performing three times from the grounding to shipment of the ginseng, the survival rate of the sea cucumber can be improved and the product can be shipped early. In other words, the seedling production period of Korean sea cucumber was conventionally from late May to July, but the artificial seedling of sea cucumber was produced by managing the fresh cucumber according to the management method of the present invention, It is now possible to produce seedlings. FIG. 7 is a seedling production confirmation form of seeds produced through the management of wild ginseng according to the present invention.

According to the method of producing the early seedling of the sea cucumber according to the present invention, it is possible to secure a good quality sea cucumber seedling by increasing the initial cultivation period by changing the production period of the artificial seedling of the sea cucumber, It is possible to realize the early seedling production of sea cucumber and not only to expand the period in which seed production can be made but also to shorten the cultivation period of sea cucumber seeds and thus to contribute to the increase in income of the sea cucumber cultured fish market.

Claims

Production of artificial seedlings using multi-yearly controlled ginseng while feeding the feeds from the aquaculture tank reduced the temperature of the raising water to 8 ℃ for 12 weeks and then raised to 18 ℃ for 14 weeks (a);
(B) scattering and stabilizing the raw ginseng in step (a) with scattering stimulation through water temperature stimulation, surface stimulation, and dry stimulation;
(C) generating the embryo obtained in the step (b) in a water tank;
(B) growing the ginseng in step (c) until it grows to 0.5 g; And
And (d) raising ginseng in a special mange when the ginseng is grown to 0.5 g in step (d). The method for controlling the production time of sea cucumber artificial seeds

The method of claim 1, wherein the step (a)
(A) lowering the natural water temperature to 16.5 ° C for 4 weeks;
(B) lowering to 10.3 캜 for 4 weeks from the step (A);
(C) lowering to 8 占 폚 for 4 weeks from the step (B);
(D) raising from 0.5 ° C to 9.5 ° C for 3 weeks each week from the step (C);
(E) raising from 17.5 占 폚 to 18 占 폚 for eight weeks at 1.0 占 폚 every week from the step (D);
And maintaining (F) the step (E) from the step (F) to the spawning induction at 18 ° C.

[2] The method of claim 2, wherein the step (d) comprises: (1) placing the wipes horizontally on the bottom of the water tank for one month;
After step (1), the step (2) of setting up the ripples vertically on the bottom of the water tank until the gills are grown to 0.5 g; A method for regulating the production timing of sea cucumber artificial seeds through the land management of the mother sea cucumber

[Claim 4] The method according to claim 3, wherein the crude ginseng feed is a dry solid feed obtained by mixing the feed composition and fermenting for 12 hours to cut and cut into a predetermined area, and feeding the ginseng to 3 to 6 wt% How to control the production time of sea cucumber seedlings through the management of sea cucumbers