CN108142328B - Early propagation and rapid seedling raising method for small yellow croakers - Google Patents
Early propagation and rapid seedling raising method for small yellow croakers Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
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- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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Abstract
The invention relates to the technical field of breeding and breeding of marine culture fishes, in particular to a method for early breeding and rapid breeding of small yellow croakers. By the method for early breeding the small yellow croakers, the spawning and fertilization time of the small yellow croakers can be advanced by 1 month compared with the natural condition, the spawning and fertilization time is 88.2 +/-0.2%, the fertilization rate is 78.4 +/-0.1%, the hatching rate is 76.7 +/-0.1%, the breeding time of the small yellow croakers with the body length of 5cm is shortened by 50 days compared with the breeding time under the natural condition, the average body length of the small yellow croakers at the beginning of 6 months reaches 7.8cm, the survival rate is improved by 20%, the purposes of early emergence and early marketing of the small yellow croakers are achieved, the price of the small yellow croakers is high, the economic benefit is good, the market demand on large-size small yellow croakers products can be relieved, and a way for protecting the fishery resources of the east China sea small yellow cro.
Description
Technical Field
The invention relates to the technical field of breeding and seedling raising of marine culture fishes, in particular to a method for early breeding and quickly raising small yellow croakers.
Background
The small yellow croaker, the large yellow croaker, the hairtail and the cuttlefish are called four marine products in China, belong to Osteichthyes, Perciformes, Scypharochadae and yellow croaker, and are important economic fishes in China coastal region. The distribution range is wide, and the method is mainly concentrated in sea areas with the water depth of less than 100 m in Bohai sea, yellow sea, east sea and West shore sea areas of Korean peninsula. With the continuous development of fishery equipment and fishing technology, the small yellow croaker resources are seriously threatened by over-fishing, and the influence of factors such as environmental deterioration and the like causes the fishing yield of the small yellow croakers to be rapidly reduced, and the small yellow croaker fishery population structure also has changes such as low age, miniaturization, premature sexual maturity and the like. In addition, the yellow croakers can be sexually mature at the age of 1, the yellow croakers in the Zhoushan fishing place begin to lay eggs and breed in the middle ten days of April, the weight of most of the yellow croakers is less than 100 g/fish after the growth of one season in the fishing season to the market, the specification is small, the market price is low, the economic effect is not obvious, and the market price of a few of the yellow croakers with the weight of more than 150 g/fish is 2-3 times higher than that of the yellow croakers. Although the existing method for the full-artificial propagation of the small yellow croakers exists, the method has the defects of time and labor consumption, high production cost, low survival rate, long time for putting the fry on the market, great influence of seasons and the like, and is not beneficial to realizing early emergence, early sale and high fry price. Therefore, the cultivation of the artificially cultured small yellow croaker with rapid growth and large specification is a technical problem which needs to be overcome at present.
Chinese patent CN201410475021.9, the patent name of which is a breeding method for breeding and cultivating large-size labiatae herba in advance, discloses a method for breeding large-size labiatae herba by breeding and cultivating early, which comprises the processes of parent fish breeding, parent fish ripening promotion, artificial spawning promotion, artificial insemination, incubation, fry breeding and the like, but the method is only suitable for the labiatae herba and has poor applicability to small yellow croakers.
Disclosure of Invention
Aiming at the problems that most of the existing small yellow croakers are small in emergence specification and unobvious in economic benefit, the invention provides a method for early breeding and quick breeding of the small yellow croakers.
The invention provides the following technical scheme:
a method for early breeding and rapid breeding of little yellow croaker comprises the following steps:
(1) disinfecting the parent fish pond and the hatching tank, then washing the parent fish pond and the hatching tank clean by sand-filtered seawater, and then injecting the sand-filtered seawater;
(2) selecting male parent fish and female parent fish which have no trauma on body surface and normally eat and have the weight of 60-100 g in the last ten days of 1 month, polyculturing in a parent fish pond according to the proportion of 1:1, and gradually replacing artificial mixed feed fed before polyculture with clamworm;
(3) gradually heating the water temperature to 15.5 ℃ in the middle ten days of the month, keeping the temperature constant, quantitatively changing the seawater in the parent fish pond every day, and prolonging the illumination;
(4) checking the gonad maturity of the parent fish from the first 3 th to the middle 3 th of the month, and injecting oxytocin injection into the parent fish after the requirements are met so that the parent fish spawns and fertilizes in the parent fish pond;
(5) collecting fertilized eggs in the parent fish pond, and placing the fertilized eggs in an incubation cylinder for conventional incubation to obtain fish fries;
(6) after 10-15 days of incubation, gradually raising the temperature of the seawater in the incubation tank to 28 ℃ and keeping, and simultaneously carrying out split-tank seedling culture along with the increase of the body length of the fry.
As an improvement of the method, the clamworm completely replaces the artificial compound feed within 5 days in the step (2), and the daily feeding mass ratio of the clamworm to the artificial compound feed is as follows in sequence: 1:4, 2:3, 1:1, 4:1 and 5: 0.
As an improvement of the method of the invention, the temperature rise rate in the step (3) is 0.5 ℃/day, 30-40% of the volume of seawater in the parent fish pond is replaced by 16 ℃ sand-filtered seawater every day, and the total illumination time of the illumination until the parent fish pond receives the illumination including natural light illumination is prolonged to 13.5 hours.
As an improvement of the method of the invention, the gonad maturity of the parent fish injectable oxytocin injection in the step (4) is as follows: the gonads of the female fishes are full, and granular egg cells can be seen in the gonads; the gonads of the male fishes are full and milk white, and sperm overflows when the gonads are slightly squeezed.
As an improvement of the method, the oxytocin injection in the step (4) is a compound amino acid solution of luteinizing hormone releasing hormone and human chorionic gonadotropin, the concentration of the luteinizing hormone releasing hormone is 1.2 mu g/kg, the concentration of the human chorionic gonadotropin is 500IU/kg, the injection dosage of female fish is 0.2mL, and the injection dosage of male fish is 0.1 mL.
As an improvement of the method of the invention, in the step (5), the stocking density of the fries in the hatching tank is 2 tails per liter of seawater.
As an improvement of the method, the time interval between the step (4) of injecting the oxytocin injection into the parent fish and the step (5) of collecting the fertilized eggs is 36-48 h.
As an improvement of the method, the temperature rise rate of the step (5) is 1 ℃/day, and the method for raising seedlings by cylinders comprises the following steps: dividing the fry into 1cm long cylinders, and stocking with seawater of 1 tail/L density; when the seedling body grows to 3cm, the pots are separated, and the stocking density is 1 tail/2L of seawater.
As an improvement of the method, the parent fish pond is an indoor semi-buried cement pond, the pond depth of the parent fish pond is 1.8-2 m, the water depth is 1m, and the water capacity is 20m3The top of the parent fish pond is provided with a heat-insulating film and a lighting lamp, the heat-insulating film covers the area of the pond surface 4/5, the power of the lighting lamp is 12W, and the arrangement density is 1/10 m2。
As an improvement of the method, the salinity of the sand-filtered seawater is 28-32 per mill.
The method for quickly breeding the small yellow croakers realizes the early emergence of the seedlings of the small yellow croakers through the early propagation of the small yellow croakers and the reasonable culture of fish fries.
Selecting male parent fish and female parent fish of little yellow croaker which have no trauma on body surface, normally eat and actively move in 1 month to perform nutrition enhancement in the hydrophilic pond. In the process of nutrition enhancement, the clamworm is used for gradually replacing the artificial mixed feed fed before polyculture, thereby enhancing the physique of parent fish, promoting the growth of little yellow croaker, and gaining weight. Heating the seawater in the parent fish pond to 15.5 ℃ at the heating rate of 0.5 ℃/day in the middle ten (2) month for further intensive culture for 10-15 days, wherein the gonads of the small yellow croakers are mature during the period from the first ten (3) month to the middle ten (3) month for induced spawning, and collecting and hatching fertilized eggs. Under natural conditions, the water temperature of the offshore sea area in 1 month is 10 ℃, the small yellow croakers keep a certain activity degree, the water temperature of the seawater is reduced to 8 ℃ after the small yellow croakers enter 2 months, the movement and the food intake of the small yellow croakers are further reduced, and the nutrition enhancement of the small yellow croakers is not facilitated, so that the nutrition enhancement feeding of parent small yellow croakers is selected in 1 month. The weight of parent fish of the small yellow croaker is 60-100 g, the small yellow croaker with small weight is relatively weak, and the fish with large weight can be 2-year-old fish. After feeding and strengthening for a period of time, raising the temperature of the water in the parent fish pond to 15.5 ℃ in the middle ten days of 2 months at a gradient of 0.5 ℃/day, gradually adapting the body of the little yellow croaker to the water temperature through slow temperature rise and fully developing, after the illumination time is prolonged and the nutrition is continuously strengthened for 10-15 days to the middle ten days of 3 months, the gonad of the little yellow croaker reaches maturity, and then injecting oxytocin injection liquid to perform spawning induction on the little yellow croaker and fertilization to form fertilized eggs. Compared with the fertilization of the yellow croaker starting spawning in the middle ten days of 4 months under natural conditions, the fertilization time is advanced by about 1 month. The oxytocin injection is a compound amino acid solution of luteinizing hormone releasing hormone (Lhrh-A2) and human chorionic gonadotropin (HGG), wherein HCG directly stimulates the gonad of the yellow croaker to release follicle stimulating hormone and luteinizing hormone, and promotes the maturation and discharge of ova and sperms, Lhrh-A2 promotes ovulation by acting on the pituitary of the yellow croaker, and Lhrh-A2 and HGG are compounded to effectively improve the spawning rate of exogenous hormone insensitive parent fish, the spawning rate of the yellow croaker obtained by the process is 88%, the fertilization rate is 78%, and the hatching rate of transferring fertilized eggs into a hatching tank is 76%. And after incubation for 10-15 days, the natural seawater temperature reaches 11 ℃, the seawater temperature in the incubation cylinder is increased to 28 ℃ at the speed of 1 ℃/day, the time is 4 in the middle of ten days, the fry of the yellow croaker at 28 ℃ is kept to grow rapidly, and the fry is cultivated in different cylinders in the growth process. Through the early propagation and seedling raising processes, the average body length of the fry of the small yellow croaker is 7.8cm in the last ten days of 6 months, compared with the natural condition, the breeding time of the fry with the body length of 5cm is shortened by 50 days, the survival rate is improved by 20 percent, thereby shortening the fry emergence time of the small yellow croaker and realizing the aims of early fry emergence, early marketing and high price of the small yellow croaker.
The invention has the following beneficial effects:
the invention provides an artificial early fry breeding method for small yellow croakers, the spawning and fertilization time of the small yellow croakers is advanced by 1 month compared with the natural condition, the spawning and fertilization rate is 88.2 +/-0.2%, the fertilization rate is 78.4 +/-0.1%, the hatching rate is 76.7 +/-0.1%, the average length of the fry in the first ten days of 6 months reaches 7.8cm, the fry breeding time of the small yellow croakers with the length of 5cm is shortened by 50 days compared with the fry breeding under the natural condition, the survival rate is improved by 20%, so that the aims of early emergence of the small yellow croakers and early marketing are fulfilled, the fry price of the small yellow croakers is high, the economic benefit is good, meanwhile, the market demand on large-size small yellow croakers is relieved, and a way for protecting fishery resources of the small yellow croakers in the east China is provided.
Drawings
FIG. 1 is a technical scheme of the process of the present invention.
FIG. 2 is a graph showing the comparison of the body length of fry at different incubation temperatures.
FIG. 3 is a statistical representation of the effect of different incubation temperatures on fry length.
Detailed Description
The following further describes the embodiments of the present invention.
The starting materials used in the present invention are commercially available or commonly used in the art, unless otherwise specified, and the methods in the following examples are conventional in the art, unless otherwise specified.
The salinity of the sand-filtered seawater used by the method is 28-32 per mill.
The parent fish pond used in the method is located in a Xixuan fishery science and technology island in the Zhoushan sea area and is an indoor semi-buried cement pond, the depth of the parent fish pond is 1.8-2 m, the water depth is 1m, and the water capacity is 20m3The top of the parent fish pond is provided with a heat preservation film and a lighting lamp, the heat preservation film covers the area of the pond surface 4/5, the heat preservation film is two layers of black mulching films, the power of the lighting lamp is 12W, and the arrangement density of the lighting lamp is 1/10 m2。
The heating device used in the method is a heating rod, and the density of the heating rod arranged in the parent fish pond is 1 heating rod/2 m2。
In the method, the time interval between the injection of the oxytocin injection to the parent fish and the collection of the fertilized eggs is 36-48 h.
As shown in FIG. 1, the technical route of the method of the invention is as follows: selecting male parent fish and female parent fish of the yellow croaker in the first ten days of 1 month to perform mixed culture in the parent fish pond according to the quantity of 1:1, keeping the seawater salinity of 28-32 per mill in the parent fish pond, feeding clamworm for nutrition-enhanced culture, then raising the water temperature to 15.5 ℃ at the rate gradient of 0.5 ℃/day and prolonging the illumination time to 13.5 hours to enable the parent fish to form an early breeding group, fertilizing 1 month ahead of the natural state, incubating and culturing fertilized eggs in an incubation tank, raising the seawater temperature to 28 ℃ at the rate gradient of 1 ℃/day, culturing for two months to the first 6 months, transferring the natural seawater temperature to the culture pond to perform conventional culture to form adult fish of the yellow croaker when the natural seawater temperature reaches 28 ℃, enabling the adult fish to have a large size compared with the natural condition, and then selecting female and male fish of the yellow croaker which are actively moving and normally eating as the male parent fish and female fish of the yellow croaker from the female fish to perform nutrition-enhanced culture in the parent fish pond again, and carrying out early propagation and rapid seedling culture of the yellow croaker in the next round.
Examples
A method for early breeding and rapid breeding of little yellow croaker comprises the following steps:
(1) preparing a parent fish pond and an incubation tank in the last 12 th month each year, sprinkling 700mg/L sodium hypochlorite solution in the parent fish pond and the incubation tank for disinfection, then washing the parent fish pond and the incubation tank clean by sand-filtered seawater, and then injecting the sand-filtered seawater;
(2) the temperature of the seawater in the Zhoushan sea area in the first ten days of 1 month is 10 ℃, male parent fishes and female parent fishes which have no trauma on the body surface and are normally fed and actively move are selected to be mixedly cultured in the parent fish pond according to the proportion of 1:1, the weight of the parent fishes is 80 g/fish, the stocking density is 600 tails/parent fish pond, clamworms are used for gradually replacing artificial mixed feed which is fed before mixotropy within 5 days, and the daily feeding mass ratio of the clamworms to the artificial mixed feed is sequentially as follows: 1:4, 2:3, 1:1, 4:1 and all clamworms, and the feeding is carried out for 3 times per day with the full feeding, and the total feeding amount is 1.5 percent of the weight of the small yellow croaker;
(3) 2, in the middle ten days of the month, the temperature of the sea water in the Zhoushan area is 8 ℃, the water temperature is increased to 15.5 ℃ at the rate of 0.5 ℃/day for 15 days, the temperature is kept constant, 30% of the volume of the sea water in the parent fish pond is replaced by 16 ℃ of sand-filtered sea water every day, and the illuminating lamp is turned on to prolong the illumination time so that the total illumination time, including natural light illumination, received by the parent fish pond is 13.5 hours;
(4) checking the gonad maturity of the parent fish in the middle 3 th of the month, when the gonad of a female fish is full, granular egg cells visible in the gonad and the gonad of a male fish are full and milky, and the gonad is slightly extruded and the sperm overflow is visible, injecting oxytocin injection liquid to the basal part of the pectoral fin of the parent fish at 18:00 of the first day, collecting fertilized eggs at 6:00 of the third day, wherein the oxytocin injection liquid is a compound amino acid solution of luteinizing hormone releasing hormone and human chorionic gonadotropin, the concentration of the luteinizing hormone releasing hormone is 1.2 mu g/kg, the concentration of the human chorionic gonadotropin is 500IU/kg, the injection dosage of the female fish is 0.2mL, and the injection dosage of the male fish is 0.1 mL;
(5) putting the fertilized eggs collected in the parent fish pond into an incubation cylinder for conventional incubation to obtain fish fries, wherein the stocking density of the fish fries is 2 tails per liter of seawater;
(6) after 10 days of incubation, the temperature of the seawater in the incubation tank is raised to 28 ℃ at 1 ℃/day to cultivate the fry, and the fry is divided into tanks along with the increase of the body length of the fry, wherein the method comprises the following steps: the fry is grown for 1cm and is put in a jar once, and the stocking density is 1 tail/L seawater; the seedlings grow to 3cm in the jar once, and the stocking density is 1 tail/2L of seawater;
(7) transferring the fry to a culture pond for conventional culture when the water temperature of natural seawater in the Zhoushan sea area reaches 28 ℃ in 6 months to obtain adult small yellow croakers, and using the adult small yellow croakers as a parent fish source for early breeding and rapid seedling culture of the next small yellow croakers.
The spawning and fertilization time of the small yellow croakers obtained by the method is 1 month earlier than that of the small yellow croakers obtained by the natural condition, the spawning and fertilization rate is 88.2 +/-0.2%, the fertilization rate is 78.4 +/-0.1%, the hatching rate is 76.7 +/-0.1%, the fry breeding time of the small yellow croakers with the body length of 5cm is shortened by 50 days compared with that of the small yellow croakers cultured under the natural condition, the average body length of the fry in the first 6 months reaches 7.8cm, and the survival rate is improved by 20%.
It should be noted that, in the above embodiments and not limited to the above embodiments, in step (2), the other suitable stocking density for putting the parent fish in the parent fish pond is less than 600 tails/parent fish pond and not less than 400 tails/parent fish pond, the weight of the other suitable parent fish is less than 80 g/strip but not less than 60 g/strip or more than 80 g/strip but not more than 100 g/strip, and the other suitable times of feeding for satiety is 2 times per day; in the step (3), the other proper volume for replacing the parent fish pond seawater every day is more than 30% but not more than 40%; in the step (4), the time for checking the gonad maturity of other parent fishes is 3 ten days; the difference between the statistic values of the spawning rate, the fertility rate and the hatchability of the parent fish obtained by early breeding of the young yellow croaker by adopting the parameters and the embodiment is not more than 2 percent. And (3) the temperature of the natural seawater after incubation for 10-15 days in the step (6) is 11 ℃, and the influence of the seawater temperature rise for 11 days, 12 days, 13 days, 14 days or 15 days after incubation on the fry growth by the seawater temperature rise for 1 ℃/day and the seawater temperature rise for 10 days after incubation is not more than 1%. In the step (7), the fry can be transferred to a culture pond for conventional culture when the water temperature of the natural seawater reaches 22 ℃.
Influence of fry rearing temperature on growth of fish body
Control group: the difference from the above-mentioned embodiment (raising the incubation temperature at a rate of 1 ℃/day up to 28 ℃ and maintaining it after incubation 10) is that the temperature of seawater in the incubation tank after fry incubation is kept in synchronization with the natural seawater temperature;
16 ℃ group: the difference from the above-described example (raising the incubation temperature at a rate of 1 ℃/day up to 28 ℃ and maintaining it after 10 days of incubation) is that the temperature of seawater in the incubation tank is raised at 1 ℃/day up to 16 ℃ after 10 days of incubation of the fry;
22 ℃ group: the above-mentioned example (raising the rearing temperature at a rate of 1 ℃/day up to 28 ℃ and maintaining after the hatching of 10) differs in that the rearing of the fry is maintained after 10 days of the fry hatching with the seawater temperature in the hatching tank raised at a rate of 1 ℃/day up to 22 ℃.
Example comparison of individual body length of fry after 40 days incubation with control, 16 ℃ and 22 ℃ groups is shown in fig. 2, and statistical analysis of body length characteristics of different incubation temperature populations is shown in fig. 3, using a one-way ANOVA followed by Duncan's post hoc test.
As can be seen from FIG. 1, the length of the fry of yellow croaker raised to 28 ℃ after 10 days of incubation is longest, followed by the fry raised to 22 ℃, followed by the fry kept at the natural seawater temperature, and then the length of the fry raised to 16 ℃ is smallest.
As can be seen from fig. 2, the body length of the small yellow croaker fry bred at 28 ℃ is significantly higher than that of the small yellow croaker fry bred at 22 ℃; the body length of the fry of the yellow croaker cultured at 22 ℃ is obviously higher than that of the fry of the control group; the body length of the fish fry of the control group is obviously higher than that of the fish fry of the yellow croaker cultured at 16 ℃; the different lower case letters on the error bars represent statistical differences at P <0.05 values.
Claims (7)
1. A method for early breeding and rapid breeding of little yellow croaker comprises the following steps:
(1) disinfecting the parent fish pond and the hatching tank, then washing the parent fish pond and the hatching tank clean by sand-filtered seawater, and then injecting the sand-filtered seawater;
(2) selecting male parent fish and female parent fish which have no trauma on body surface and normally eat and have the weight of 60-100 g in the last ten days of 1 month, polyculturing in a parent fish pond according to the proportion of 1:1, and gradually replacing artificial mixed feed fed before polyculture with clamworm;
(3) gradually heating the water temperature to 15.5 ℃ in the middle ten days of the month, keeping the temperature constant, quantitatively changing the seawater in the parent fish pond every day, and prolonging the illumination;
(4) checking the gonad maturity of the parent fish from the first 3 th to the middle 3 th of the month, and injecting oxytocin injection into the parent fish after the requirements are met so that the parent fish spawns and fertilizes in the parent fish pond;
(5) collecting fertilized eggs in the parent fish pond, and placing the fertilized eggs in an incubation cylinder for conventional incubation to obtain fish fries;
(6) after 10-15 days of incubation, the natural seawater reaches 11 ℃, gradually raising the temperature of the seawater in the incubation tank to 28 ℃ and keeping the temperature for the middle and upper ten days in 6 months, transferring the seawater to a culture pond for conventional culture when the temperature of the natural seawater reaches 28 ℃, and simultaneously carrying out split-tank seedling culture along with the increase of the body length of the fry;
the temperature rise rate in the step (3) is 0.5 ℃/day, 30-40% of seawater in the parent fish pond is replaced by 16 ℃ sand-filtered seawater every day, and the total illumination time of illumination until the parent fish pond receives the illumination including natural light illumination is prolonged to 13.5 hours;
the temperature rise rate of the step (6) is 1 ℃/day, and the method for raising seedlings in different jars comprises the following steps: dividing the fry into 1cm long cylinders, and stocking with seawater of 1 tail/L density; dividing the fry into 3cm long, stocking with seawater of density 1 tail/2L;
the salinity of the sand-filtered seawater is 28-32 per mill.
2. The method for the early propagation and the rapid seedling raising of the small yellow croaker according to claim 1, wherein the clamworm completely replaces the artificial mixed feed within 5 days in the step (2), and the mass ratio of the clamworm to the artificial mixed feed every day is as follows: 1:4, 2:3, 1:1, 4:1 and 5: 0.
3. The method for early breeding and fast breeding of small yellow croakers according to claim 1, wherein the gonad maturity of the parent fish injected with oxytocin injection in the step (4) is as follows: the gonads of the female fishes are full, and granular egg cells can be seen in the gonads; the gonads of the male fishes are full and milk white, and sperm overflows when the gonads are slightly squeezed.
4. The method for early breeding and fast breeding of yellow croaker according to claim 1, wherein the oxytocin injection in step (4) is a compound amino acid solution of luteinizing hormone releasing hormone and human chorionic gonadotropin, the luteinizing hormone releasing hormone concentration is 1.2 μ g/kg, the human chorionic gonadotropin concentration is 500IU/kg, the female fish injection dosage is 0.2mL, and the male fish injection dosage is 0.1 mL.
5. The early propagation and rapid seedling raising method for yellow croaker according to claim 1, wherein the stocking density of the fry in the hatching tank in step (5) is 2 fish/L seawater.
6. The method for early propagation and rapid seedling raising of the small yellow croaker according to the step 1 or 4 is characterized in that the time interval between the step (4) of injecting oxytocin injection into the parent fish and the step (5) of collecting fertilized eggs is 36-48 h.
7. The method for early breeding and rapid breeding of yellow croaker according to claim 1, wherein the parent fish pond is an indoor semi-buried cement pond, the depth of the parent fish pond is 1.8-2 m, the depth of water is 1m, and the water capacity is 20m3The top of the parent fish pond is provided with a heat-insulating film and a lighting lamp, the heat-insulating film covers the area of the pond surface 4/5, the power of the lighting lamp is 12W, and the arrangement density is 1/10 m2。
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| CN112243896A (en) * | 2020-10-20 | 2021-01-22 | 中国水产科学研究院淡水渔业研究中心 | Artificial breeding method for freshwater grouper |
| CN112586408B (en) * | 2020-11-19 | 2022-06-21 | 浙江省农业科学院 | Method for identifying optimal spawning induction time of small yellow croakers |
| CN112998158B (en) * | 2021-03-19 | 2024-02-06 | 象山港湾水产苗种有限公司 | Wet feed for improving reproductive performance of parent fish of small yellow croaker and feeding method |
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| CN104904636B (en) * | 2015-06-10 | 2018-06-19 | 浙江省海洋水产研究所 | A kind of induced spawning method of little yellow croaker |
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| CN108142328A (en) | 2018-06-12 |
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