CN106962235B - Early propagation method of siganus guttatus - Google Patents
Early propagation method of siganus guttatus Download PDFInfo
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- 241000317936 Siganus guttatus Species 0.000 title claims abstract description 18
- 238000000034 method Methods 0.000 title claims abstract description 16
- 238000009395 breeding Methods 0.000 claims abstract description 27
- 230000001488 breeding effect Effects 0.000 claims abstract description 24
- 241000251468 Actinopterygii Species 0.000 claims description 43
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 23
- 235000013601 eggs Nutrition 0.000 claims description 11
- 150000001875 compounds Chemical class 0.000 claims description 10
- 239000013535 sea water Substances 0.000 claims description 10
- 238000004321 preservation Methods 0.000 claims description 9
- 239000008239 natural water Substances 0.000 claims description 8
- 239000002689 soil Substances 0.000 claims description 8
- XNOPRXBHLZRZKH-UHFFFAOYSA-N Oxytocin Natural products N1C(=O)C(N)CSSCC(C(=O)N2C(CCC2)C(=O)NC(CC(C)C)C(=O)NCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(CCC(N)=O)NC(=O)C(C(C)CC)NC(=O)C1CC1=CC=C(O)C=C1 XNOPRXBHLZRZKH-UHFFFAOYSA-N 0.000 claims description 7
- 101800000989 Oxytocin Proteins 0.000 claims description 7
- 102100031951 Oxytocin-neurophysin 1 Human genes 0.000 claims description 7
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- XNOPRXBHLZRZKH-DSZYJQQASA-N oxytocin Chemical compound C([C@H]1C(=O)N[C@H](C(N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CSSC[C@H](N)C(=O)N1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(C)C)C(=O)NCC(N)=O)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 XNOPRXBHLZRZKH-DSZYJQQASA-N 0.000 claims description 7
- 229960001723 oxytocin Drugs 0.000 claims description 7
- CPKVUHPKYQGHMW-UHFFFAOYSA-N 1-ethenylpyrrolidin-2-one;molecular iodine Chemical compound II.C=CN1CCCC1=O CPKVUHPKYQGHMW-UHFFFAOYSA-N 0.000 claims description 6
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 6
- 229920000153 Povidone-iodine Polymers 0.000 claims description 6
- 239000004568 cement Substances 0.000 claims description 6
- 230000002354 daily effect Effects 0.000 claims description 6
- 201000010099 disease Diseases 0.000 claims description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 6
- 230000003203 everyday effect Effects 0.000 claims description 6
- 229960001621 povidone-iodine Drugs 0.000 claims description 6
- 235000016709 nutrition Nutrition 0.000 claims description 5
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- 238000010521 absorption reaction Methods 0.000 claims description 4
- 230000033228 biological regulation Effects 0.000 claims description 4
- 230000006378 damage Effects 0.000 claims description 4
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- 239000004576 sand Substances 0.000 claims description 4
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- AYIRNRDRBQJXIF-NXEZZACHSA-N (-)-Florfenicol Chemical compound CS(=O)(=O)C1=CC=C([C@@H](O)[C@@H](CF)NC(=O)C(Cl)Cl)C=C1 AYIRNRDRBQJXIF-NXEZZACHSA-N 0.000 claims description 2
- PZJWYUDBXNNVLZ-UHFFFAOYSA-N 1-cyclopropyl-7-(4-ethylpiperazin-1-yl)-6-fluoro-4-oxoquinoline-3-carboxylic acid;hydrochloride Chemical compound Cl.C1CN(CC)CCN1C(C(=C1)F)=CC2=C1C(=O)C(C(O)=O)=CN2C1CC1 PZJWYUDBXNNVLZ-UHFFFAOYSA-N 0.000 claims description 2
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 claims description 2
- 241000392375 Sinonovacula constricta Species 0.000 claims description 2
- 241000630524 Taractes rubescens Species 0.000 claims description 2
- 239000004744 fabric Substances 0.000 claims description 2
- 229960003760 florfenicol Drugs 0.000 claims description 2
- 238000005286 illumination Methods 0.000 claims description 2
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 claims description 2
- 229910052740 iodine Inorganic materials 0.000 claims description 2
- 239000011630 iodine Substances 0.000 claims description 2
- 210000000006 pectoral fin Anatomy 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- 230000003449 preventive effect Effects 0.000 claims description 2
- 210000002149 gonad Anatomy 0.000 claims 1
- 230000008901 benefit Effects 0.000 abstract description 8
- 238000005516 engineering process Methods 0.000 abstract description 3
- 238000009360 aquaculture Methods 0.000 abstract 1
- 244000144974 aquaculture Species 0.000 abstract 1
- 230000017448 oviposition Effects 0.000 description 16
- 229940088597 hormone Drugs 0.000 description 9
- 230000012447 hatching Effects 0.000 description 8
- 239000005556 hormone Substances 0.000 description 8
- 230000018109 developmental process Effects 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 241001274978 Siganus Species 0.000 description 2
- 241001274981 Siganus canaliculatus Species 0.000 description 2
- 241000237538 Solenidae Species 0.000 description 2
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- 230000002196 ecbolic effect Effects 0.000 description 2
- 230000033764 rhythmic process Effects 0.000 description 2
- 241001474374 Blennius Species 0.000 description 1
- 102000011022 Chorionic Gonadotropin Human genes 0.000 description 1
- 108010062540 Chorionic Gonadotropin Proteins 0.000 description 1
- 108700012941 GNRH1 Proteins 0.000 description 1
- 239000000579 Gonadotropin-Releasing Hormone Substances 0.000 description 1
- 241000276618 Perciformes Species 0.000 description 1
- 241001290310 Sciaenops Species 0.000 description 1
- 229940015047 chorionic gonadotropin Drugs 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 230000026109 gonad development Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000009364 mariculture Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002863 oxytocic agent Substances 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention relates to the field of aquaculture, in particular to a method for early breeding siganus guttatus. The invention has the advantages that: the method has the advantages of strong operability, high spawning rate, little influence by seasons, early seedling emergence, capability of being listed in advance and high seedling price; the cultured siganus guttatus early-breeding seedlings have long growth period and large marketing specification, and can obviously improve the economic benefit. The technology is mature, safe and easy to master, can be quickly digested and mastered by farmers, and is suitable for developing large-scale artificial breeding of seedlings.
Description
Technical Field
The invention relates to the field of fish culture, in particular to a method for early propagation of siganus guttatus.
Background
Siganus guttatus (commonly known as sciaenops aurea) belongs to the family of Perciformes, the family of Siganus, the genus of Siganus, and has delicious meat, high protein content, rich n-3 highly unsaturated fatty acid and high nutritional value, and is deeply loved by consumers. The siganus oramin has the characteristics of omnivorous property, euryhaline property and the like, is eaten by macroalgae under natural conditions, is also a rare omnivorous fish in mariculture varieties, and meets the requirement of the development of the current healthy culture mode in China. The siganus oramin is used as a good new breeding object, and has a wide development and utilization prospect. The egg laying period of the siganus guttatus is 4-7 months per year under natural conditions, and the seven to nine lunar calendars lay eggs every month. If the siganus guttatus is produced according to the natural spawning rhythm, the following problems can be caused; 1. the seedlings are listed in batches and intensively, and the price of the seedlings is low; 2. the produced fry grows about half a year and is listed before spring festival, and the price of the concentrated adult fishes listed is not high; 3. short growth period, small finished fish marketing specification, low yield and low economic benefit.
Therefore, the research shows that the seed has high spawning rate, little influence by seasons, early emergence of seedlings, early marketing and high seed price; the cultivation period is long, the market specification is large, and a method capable of obviously improving economic benefits is very necessary.
Disclosure of Invention
The invention aims to provide a method for early breeding siganus guttatus, which cultures early breeding seedlings of siganus guttatus required by cultivation and production by technical means of parent fish selection, temperature regulation, nutrition enhancement, hormone induced spawning and the like. The method has strong operability, the cultured early-breeding seedlings of the siganus guttatus have long growth period and large marketing specification, and can obviously improve the economic benefit. The technology is mature, safe and easy to master, can be quickly digested and mastered by farmers, and is suitable for developing large-scale artificial breeding of seedlings.
In a first aspect of the present invention, a method for early propagation of siganus guttatus is provided, which comprises the following steps:
(A) preparation of a culture pond: an indoor semi-buried cement pond is adopted as a parent fish culture pond, a heat-preservation mulching film is covered on the top of the pond, the area of the pond accounts for 4/5 of the bottom of the whole culture pond, an air head is placed, seawater filtered by sand is injected into the culture pond after the culture pond is disinfected, the height of the water level is 1m, the salinity is 27-32 per mill, and a thermostat is equipped for one machine;
(B) selection of early breeding parent fish: selecting early breeding parent fish of siganus guttatus after the natural water temperature is lower than 22 ℃ every year; selecting individuals without injury, with smooth body surfaces and weight of more than 400g as parents, and transferring the individuals to an early breeding parent breeding pool after disinfection treatment, wherein the number of the parents in each pool is 200;
(C) and (3) nutrition fortification: adding fresh and live baits on the basis of artificial compound feed, and feeding twice a day;
(D) temperature regulation and control: when the natural water temperature is lower than 22 ℃, raising the temperature to 29 ℃ in a range of 0.5-1 ℃ per day, and then maintaining constant-temperature cultivation until the water temperature of the soil pond for outdoor seedling raising is raised to more than 24 ℃; then, the temperature is reduced to be consistent with the water temperature in the soil pond for outdoor seedling culture within the range of 0.5-1 ℃ in a day, and the heat-preservation film is opened to keep natural illumination;
(E) parent fish breeding management: in the cultivation process, water is changed after dirt absorption every day, the water changing amount is 20%, the difference between the temperature of the added seawater and the temperature of the cultivation pool is not more than 1 ℃, disease control is carried out every half month, and the cultivation pools are alternately changed every month;
(F) injection of oxytocin: after the natural water temperature is stabilized above 24 ℃, injecting oxytocic hormone into parent fish with mature gonad development in the sixth lunar calendar of every month to induce spawning, allowing female parents and male parents to lay eggs in a culture pond under the dual effects of hormone induction and natural breeding rhythm to obtain fertilized eggs, and collecting the fertilized eggs to carry out conventional artificial seedling culture.
And (C) adopting an indoor semi-buried cement pond as the parent fish culture pond in the step (A), and installing an inflating device with 1 air head per square meter standard, wherein the culture pond keeps natural light.
The heat preservation film in the step (A) consists of two layers of black mulching film cloth.
The thermostat in the step (A) can be a Sensen brand thermostat.
The disinfection method in the step (A) is to sprinkle 1g/m of water in the whole pool3Povidone iodine, the content of effective iodine of povidone iodine is 1%.
The artificial compound feed in the step (C) can be a No. 5 material of a Jianma brand golden pomfret compound feed, the fresh bait is the shell-removed sinonovacula constricta, the feeding ratio of the compound feed to the fresh bait is 3:1 (mass ratio), and the daily feeding amount is 2% of the fish body weight.
And (D) covering a heat preservation shed on the soil pool for outdoor seedling culture in the step (D) to increase the water temperature to 24 ℃ as soon as possible.
The amount of the preventive drug for controlling the disease in the step (E) is 1g/m3～2g/m3The enrofloxacin hydrochloride, the florfenicol or the povidone iodine or the formaldehyde is 30g/m3It can be used alternatively, and the dosage can be increased if the disease is attacked.
In the step (F), the oxytocin is luteinizing hormone releasing hormone analogue (L HRH-A)2) Chorionic Gonadotropin (HCG) and Diosdone (DOM), selected from Ningbo Sansheng hormone Co., Ltd, prepared by injecting oxytocic agent into the basal part of the pectoral fin at a dose of L HRH-A2+ HCG + DOM (5.0. mu.g +500IU +1.0mg) per kg fish body weight.
The invention has the advantages that:
the invention cultivates early breeding seedlings of siganus guttatus by the technical means of parent fish selection, temperature regulation, nutrition enhancement, hormone induced spawning and the like. The invention has high spawning rate, little influence by seasons, early emergence of seedlings, capability of appearing on the market in advance and high seedling price; the cultured siganus guttatus early-breeding seedlings have long growth period and large marketing specification, and can obviously improve the economic benefit. The technology is mature, safe and easy to master, can be quickly digested and mastered by farmers, is suitable for carrying out large-scale artificial breeding of the seeds, and is expected to promote the healthy and quick development of the artificial seed breeding work of siganus guttatus in China.
Detailed Description
The following examples are provided to illustrate specific embodiments of the present invention.
Example 1
(1) In 11/10 days in 2013, four parent fish culture ponds are prepared in the research center of the Hainan Jones, institute of offshore production, Hainan Seawall, wherein each culture pond is an indoor semi-buried cement pond, a heat preservation mulching film is covered on the top of the culture pond, seawater filtered by sand is injected, the height of the water level is 80cm, the salinity is 27-32 per thousand, and each pond is provided with one thermostat. Two incubation wells (A, B) are used for incubation and are ready for use.
(2) And in 2013, the fish is selected in 11 months and 24 days, and the natural water temperature is 22 ℃. Selecting an individual with no injury, smooth body surface and weight of more than 400g as a parent, 1g/m3And transferring the sterilized povidone iodine to an early breeding parent culture pond, wherein the number of parents in each pond is 150.
(3) After the parent is selected and placed in a culture pond to be stabilized for one day, the temperature of a culture pond A is raised to 29 ℃ within 11 months and 26 days, and is raised to 28-30 ℃ within 12 months and 3 days. And the temperature of the pond B is raised to 25 ℃ within 11 months and 26 days, and is raised to 25 ℃ within 3 days after 12 months and 12 months, and the temperature is kept at 25-27 ℃. Feeding compound feed and shelled razor clams every day, wherein the daily feeding amount is 1.5%; and changing water after dirt absorption, wherein the water changing amount is 20% every day, and the newly-entering seawater is the seawater preheated in the standby tank. And in 2014, in 28 days 1 and 28 months, after the water temperature of the soil pond for seedling culture is increased to 24.5 ℃, the temperature of the parent fish culture pond is decreased to the water temperature of the seedling culture pond by 0.5-1 ℃ in a daily range.
(4) The hormone is injected into a pool A of a afternoon culture pool in 2, 4 and 4 months in 2014 to induce spawning, and the dosage is L HRH-A2+ HCG (5.0. mu.g +1000IU) per kg fish body weight. 2, 5 days in the morning, a small amount of parent fish lay eggs, the egg laying amount is 380g, and the hatchability is 60.52%; the egg laying amount per 6 days is 770g, hatchability 75.85%; the egg laying amount in 7 days is 740g, the hatching rate is 72.59%, and the total egg laying amount is 1890 g. The conventional seedling raising method is adopted, and after 35 days of cultivation, 24.5 thousands of fish fries are produced.
The breeding pool B is injected with hormone to induce spawning in 2/4 afternoon in 2014, and the dosage is L HRH-A2+ HCG (5.0. mu.g +1000IU) per kg fish weight, parent fish failed to lay eggs.
Example 2
(1) In 11/15 days in 2014, six parent fish culture ponds are prepared in the research center of the Hainan Joghai of the institute of offshore production, the culture ponds are indoor semi-buried cement ponds, a heat preservation mulching film is covered on the top of each pond, seawater filtered by sand is injected, the height of the water level is 100cm, the salinity is 27-32 per thousand, and each pond is provided with one constant temperature machine. Four wells were used for incubation (A, B, C, D), two for use.
(2) And 6, 11 and 29 days in 2014, and selecting parent fishes at the natural water temperature of 21.4 ℃. Selecting an individual with no injury, smooth body surface and weight of more than 400g as a parent, 1g/m3And transferring the sterilized povidone iodine to an early breeding parent culture pond, wherein the number of parents in each pond is 200.
(3) After the parent is selected and placed in a culture pond to be stabilized for one day, the temperature is raised to 29 ℃ from 1 day to 12 months and 8 days, and the temperature is kept at 28-30 ℃. Feeding compound feed and shelled razor clams every day, wherein the daily feeding amount is 2.0%; and changing water after dirt absorption, wherein the water changing amount is 20% every day, and the newly-entering seawater is the seawater preheated in the standby tank. And in 2014, 2, 10 days, after the water temperature of the soil pond for seedling culture is increased to 24.0 ℃, the temperature of the parent fish culture pond is decreased to the water temperature of the seedling culture pond by 0.5-1 ℃ in a daily range.
(4) Injecting hormone to induce spawning in 24 pm of 2 months and 24 months in 2014, wherein the dosage of the A pool of the culture pool is L HRH-A2+ HCG + DOM (5.0. mu.g +500IU +1.0mg) per kg fish body weight. 2, 25 months and 25 days, the parent fish spawns, the spawning amount is 380g, and the hatchability is 65.52%; the egg laying amount in 26 days is 970g, and the hatching rate is 82.85%; the egg laying amount in 27 days is 840g, the hatching rate is 76.59 percent, and the total egg laying amount is 2190 g.
The dosage of the culture pond B is L HRH-A2+ HCG + DOM (5.0. mu.g +1000IU +1.0mg) per kg fish body weight. 2 month and 25 morning fish productEggs, wherein the egg laying amount is 420g, and the hatching rate is 60.52%; the egg laying amount in 26 days is 880g, and the hatching rate is 76.05%; the egg laying amount in 27 days is 640g, the hatching rate is 70.55%, and the total egg laying amount is 1940 g.
The dosage of the culture pond C is L HRH-A2+ HCG (5.0. mu.g +500IU) per kg fish body weight. 2, 25 months and 25 days, the parent fish lay eggs, the egg laying amount is 440g, and the hatchability is 61.22%; the egg laying amount in 26 days is 770g, and the hatching rate is 76.85%; the egg laying amount in 27 days is 640g, the hatching rate is 70.89%, and the total egg laying amount is 1850 g.
No oxytocic hormone is injected into the D pool of the culture pool, and the D pool cannot lay eggs in the month. The conventional seedling raising method is adopted to produce 85.5 thousands of fish fries in two 3 mu ponds after 35 days of cultivation.
While the preferred embodiments of the present invention have been described in detail, it will be understood by those skilled in the art that the invention is not limited thereto, and that various changes and modifications may be made without departing from the spirit of the invention, and the scope of the appended claims is to be accorded the full range of equivalents.
Claims (1)
1. A method for early propagation of siganus guttatus is characterized by comprising the following steps:
(A) preparation of a culture pond: an indoor semi-buried cement pond is used as a parent fish culture pond, a heat-insulating film covers the top of the pond, the area of the heat-insulating film accounts for 4/5 of the whole culture pond, an air head is placed, seawater filtered by sand is injected into the parent fish culture pond after the parent fish culture pond is disinfected, the water level height is 1m, and the salinity is 27-32 per mill; the parent fish culture pond adopts an indoor semi-buried cement pond, an inflation device is arranged in a standard manner of 1 air head per square meter, and the culture pond keeps natural light; the parent fish culture pond disinfection method comprises sprinkling 1g/m3Povidone iodine, wherein the content of effective iodine of the povidone iodine is 1 percent; the heat preservation film consists of two layers of black mulching film cloth;
(B) selection of early breeding parent fish: selecting early breeding parent fish of siganus guttatus after the natural water temperature is lower than 22 ℃ every year; selecting individuals without injury, with smooth body surfaces and weight of more than 400g as parents, and transferring the individuals to an early breeding parent breeding pool after disinfection treatment, wherein the number of the parents in each pool is 200;
(C) and (3) nutrition fortification: adding fresh and live baits on the basis of artificial compound feed, and feeding twice a day; the artificial compound feed is a No. 5 compound feed for the golden pomfret, the fresh bait is the shell-removed sinonovacula constricta, the feeding ratio of the compound feed to the fresh bait is 3:1, and the daily feeding amount is 2% of the fish body weight;
(D) temperature regulation and control: when the natural water temperature is lower than 22 ℃, raising the temperature to 29 ℃ in a range of 0.5-1 ℃ per day, and then maintaining constant-temperature cultivation until the water temperature of the soil pond for outdoor seedling raising is raised to more than 24 ℃; then, the temperature is reduced to be consistent with the water temperature in the soil pond for outdoor seedling culture within the range of 0.5-1 ℃ in a day, and the heat-preservation film is opened to keep natural illumination; covering a heat preservation shed on an outdoor seedling raising soil pond to enable the water temperature to be increased to 24 ℃ as soon as possible;
(E) parent fish breeding management: in the cultivation process, water is changed after dirt absorption every day, the water changing amount is 20%, the difference between the temperature of the added seawater and the temperature of the cultivation pool is not more than 1 ℃, disease control is carried out every half month, and the cultivation pools are alternately changed every month; the dosage of the preventive medicine for preventing and treating diseases is 1g/m3～2g/m3The enrofloxacin hydrochloride, the florfenicol or the povidone iodine or the formaldehyde is 30g/m3The medicine is used alternately, and the dosage can be increased if the diseases occur;
(F) injecting oxytocin, namely injecting the oxytocin into parent fish with mature gonad in the sixth lunar calendar of a month after the natural water temperature is stabilized to be more than 24 ℃ to induce the oxytocin, wherein the oxytocin is injected from the basal part of the pectoral fin, and the oxytocin is L HRH-A2HCG and DOM in a dose of L HRH-A per kg fish body weight25.0 mu g + HCG 500IU + DOM 1.0mg, and the female parent and the male parent lay eggs in a breeding pond to obtain fertilized eggs, and then the fertilized eggs are collected to carry out conventional artificial seedling culture.
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| CN108142328B (en) * | 2018-01-11 | 2020-05-05 | 浙江省海洋水产研究所 | Early propagation and rapid seedling raising method for small yellow croakers |
| CN111149735B (en) * | 2020-01-21 | 2021-07-27 | 中国水产科学研究院南海水产研究所深圳试验基地 | Large-scale cultivation method of siganus oramin fry |
| CN112219758B (en) * | 2020-10-19 | 2023-03-31 | 中国水产科学研究院南海水产研究所 | Method for controlling sexual maturity of siganus oramin |
| CN112913728A (en) * | 2021-02-02 | 2021-06-08 | 中国水产科学研究院东海水产研究所 | Efficient artificial reinforced cultivation method for pseudosciaena crocea parents |
| CN113303251A (en) * | 2021-04-21 | 2021-08-27 | 中国水产科学研究院东海水产研究所 | Efficient artificial reinforcement cultivation method for siganus longipes parents |
| CN113303255A (en) * | 2021-06-23 | 2021-08-27 | 中国水产科学研究院东海水产研究所 | Method for artificially cultivating siganus longipes parents for large-scale natural propagation |
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|---|---|---|---|---|
| CN102487866B (en) * | 2011-12-06 | 2013-06-05 | 中国水产科学研究院东海水产研究所 | Large-scale natural propagation method for artificially culturing siganus guttatus parents |
| CN104335938B (en) * | 2014-09-09 | 2016-05-11 | 中国水产科学研究院东海水产研究所 | A kind of method of siganus guttatus seed cultivation |
| CN104839070B (en) * | 2015-06-09 | 2017-10-13 | 广东海洋大学 | Method for massively culturing and facility outside a kind of many squama Xi parent populations rooms |
| CN105432524B (en) * | 2015-12-07 | 2018-10-30 | 内江师范学院 | A kind of Parabotia fasciata Dabry de Thiersant offspring seed cultivation method |
| CN105532520B (en) * | 2015-12-07 | 2019-06-04 | 内江师范学院 | A kind of expanded letter sand loach seedlings kind propagation method |
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2017
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