CN102440204B - Mixed breeding method for spotted maigre and large yellow croaker - Google Patents
Mixed breeding method for spotted maigre and large yellow croaker Download PDFInfo
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- CN102440204B CN102440204B CN2011102957339A CN201110295733A CN102440204B CN 102440204 B CN102440204 B CN 102440204B CN 2011102957339 A CN2011102957339 A CN 2011102957339A CN 201110295733 A CN201110295733 A CN 201110295733A CN 102440204 B CN102440204 B CN 102440204B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention relates to an artificial mixed breeding method for spotted maigre and large yellow croaker in marine aquaculture industry, and belongs to the technical field of marine culture. The breeding method includes the following steps, firstly pond-separated breeding maturation accelerating and parturition hastening of parent fish; secondly, same-pond breeding of spotted maigre and large yellow croaker fry; and thirdly, fry separating that's carried out through siphonage after the fry grows to be 2 to 3 cm in length. The pond-separated breeding maturation accelerating and parturition hastening of parent fish includes the maturation accelerating of the spotted maigre and the maturation accelerating of the large yellow croaker, and both the maturation accelerating of the spotted maigre and the maturation accelerating of the large yellow croaker include parent fish breeding and gonad maturation accelerating and parturition hastening. The same-pond breeding of spotted maigre and large yellow croaker fry includes same-pond hatching of the fertilized eggs of the spotted maigre and the large yellow croaker, prelarva prior period growing management, prelarva later perior growing management, postlarva period growing management and water quality regulation and control. 1.5 kg of fertilized eggs of the spotted maigre and the large yellow croaker are thrown into a mud pond of 32m<2>, and 696, 7000 juvenile fishes of 2 to 3 mm are bred at last, and the breeding rate per unit area is 2300/m<2>, as a result, the utilization rate of the breeding pond is greatly improved and the breeding cost is reduced.
Description
Technical field
The present invention relates to that spotted maigre and large yellow Crocker manually mix seedling-cultivating method in the mariculture industry, belong to agriculture mariculture technology field.
Background technology
Spotted maigre (Nibea albiflora) is commonly called as yellow mother-in-law chicken, belongs to Perciformes, Sciaenidae, Nibea, the bodily form is comparatively similar to large yellow Crocker, be lower floor fish in the coastal waters, being distributed in South China Sea, the East Sea and southern Yellow Sea, mainly inhabiting the littoral sea of sand mud substrate, is food with zoobenthos such as small-sized shell-fish and small fish, 8 ℃～33 ℃ of comfort zones, the suitableeest growth water temperature is 24 ℃～29 ℃, and age at sexual maturity was 2 ages in week, the suitable salt scope 10 ‰ of growth～40 ‰.Large yellow Crocker (Pseud osciaena cocea) is commonly called as yellow croaker, belongs to Perciformes, Sciaenidae, yellow croaker belongs to, and is lower floor fish in the coastal waters, mainly be distributed in China's southern Yellow Sea, the East Sea, the Taiwan Straits, and the South Sea, float when dawn, dusk or spring tide more, sink when daytime or neap, be food with zoobenthos such as small-sized shell-fish and small fish, 10 ℃～32 ℃ of comfort zones, the suitableeest growth water temperature is 18 ℃～25 ℃, age at sexual maturity was 2 ages in week, the suitable salt scope 17 ‰ of growth～32 ‰.Spotted maigre and large yellow Crocker all are desirable kinds that China's southeastern coast is cultured.At present, the breed that China Fujian, zhejiang and other places have been carried out these two kinds of fish has obtained favorable economic benefit.But, owing to lack systematic research, the seed rearing of spotted maigre and large yellow Crocker mainly still adopts conventional indoor cement pit (or tank) or the single variety breeding method of outdoor earth pond, namely begin till the young of long 2～3 cm of body from fertilized egg, being a certain fish in seedling water cultivates separately, the shortcoming that exists is that bait utilization is lower in the artificial breeding process, and the unit of water body emergence rate is lower, and seedling cost is higher.
These two kinds of fishes belong to equal with order, spotted maigre and large yellow Crocker are when the young of total length 20-30mm, has similitude that 20 ℃～25 ℃ of temperature and salinity 20 ‰～30 ‰ are adapted to and to the different life habits of light, be that the spotted maigre juvenile fish of total length 20-30mm perches the middle lower floor at water body, and the large yellow Crocker juvenile fish of same size perches at water body at the middle and upper levels, utilize the different habits of phase Sihe of above-mentioned two kinds of fish juvenile fish, provide possibility for the present invention mixes to grow seedlings.
Summary of the invention
At the drawback of above-mentioned prior art and spotted maigre and the large yellow Crocker habit total length 20-30mm juvenile fish, the indoor mixing seedling growing process of spotted maigre to be solved by this invention and large yellow Crocker should be set about from the following aspect: the first, and parent population branch pond is cultivated accelerating and is hastened parturition.Comprise: the parent population accelerating of (one) spotted maigre: 1, parent fish rearing; 2, gonad maturity accelerating and hastening parturition.(2) the parent population accelerating of large yellow Crocker: 1, parent fish rearing; 2, gonad maturity accelerating and hastening parturition.The second, cultivate in the same pond of spotted maigre and large yellow Crocker seed.Comprise: 1, the same pond hatching of spotted maigre and large yellow Crocker fertilized egg; 2, prelarva seedling management in early stage comprises density, changes water and bait feeding; 3, prelarva later stage seedling management comprises density, changes water and bait feeding; 4, postlarva phase seedling management comprises density, changes water and bait feeding; 5, regulating and controlling water quality.The 3rd, seed separates: when treating that seed grows to 2～3cm, adopt siphonage to carry out seed and separate.
Main summary of the invention is as follows:
One, parent population branch pond is cultivated accelerating and is hastened parturition
(1) the parent population accelerating of spotted maigre
1, the cultivation of parent population
Preceding 70 days of artificial induced spawning selects that body weight is suitable, physique is healthy and strong, not damaged, no parasitic spotted maigre, cultivates the pond by male and female than the cement that 2:1 moves into 1.5 meters of the depth of waters, and density is 1.5kg/m
3The husky filter of seawater, per 4～5m
2Floor space is put 1 gas charging stone.With around the parent fish rearing pond and top with the black cloth shading, 2 60 W fluorescent lamps are installed at 1 meter above 1.5 meters of every cultivation pond depth of waters, initial day illumination is 8 hours, it is initial day illumination time+0.5 hour after 5 days, it is initial day illumination time+0.5 hour+0.5 hour after 10 days, it is initial day illumination time+0.5 hour+0.5 hour+0.5 hour after 15 days, be every mistake 5 light light application time+0.5 hour, by that analogy ... when the same day, light application time reached 13 hours every days, keep day 13 hours every days of illumination time constant, intensity of illumination is controlled at 50 Lx～100 Lx; Parent population goes into Chi Houxian and cultivated 2 days under 10 ℃ of water temperatures, played intensification on the 3rd day, from day counting of intensification, water temperature is initial water temperature+1 ℃ after 6 days, and water temperature is initial water temperature+1 ℃+1 ℃ after 12 days, water temperature is initial water temperature+1 ℃+1 ℃+1 ℃ after 18 days, be 6 days water temperature+1 of every mistake ℃, by that analogy ..., kept 7 when waiting to rise to 14 ℃, 6 days water temperature+1 of every mistake ℃ keep constant temperature when going straight up to 22 ℃～23 ℃ again; Bait adds vitamin E for the ice fresh fish, and day throws something and feeds 1～2 time, and morning or dusk throw something and feed, and feeding rate is controlled in 3%～5% of fish body weight, and a day quantity of exchanged water is 100%;
2, gonad maturity accelerating and hastening parturition
Water temperature rises to 14 ℃, the parent population sexual gland germinates, water temperature rises to 20 ℃, check parent population gonad development situation, this moment, gonad development was the fourth phase, from pectoral fin base portion or dorsal fin base portion injection ocyodinic, the ocyodinic hormotone of LHRH-A and HCG, the raun injected dose: per kilogram fish body weight LHRH-A consumption 14 μ g add HCG consumption 130 IU; The injected dose of milter all reduces by half; Hormotone is made suspension with vitamin C and is injected.The illuminance of environment of hastening parturition should remain on 100Lx, keeps quiet around the spawning pond, and the ecbolic effect time is 35 hours, waits to lay eggs and draws in the net to collect fertilized egg after finishing.
(2) the parent population accelerating of large yellow Crocker
1, the cultivation of parent population
Preceding 50 days of artificial induced spawning is selected the large yellow Crocker of physique stalwartness, moves into the culture pond of 1.5 meters of the depth of waters by male and female respectively than 2:1, and density is 1.5kg/m
3, the husky filter of seawater, per 4～5m
2Water body is put 1 gas stone; With around the parent fish rearing pond and top with the black cloth shading, 2 60 W fluorescent lamps are installed at 1 meter above 1.5 meters of every cultivation pond depth of waters, initial day illumination is 8 hours, it is initial day illumination time+0.5 hour after 5 days, it is initial day illumination time+0.5 hour+0.5 hour after 10 days, it is initial day illumination time+0.5 hour+0.5 hour+0.5 hour after 15 days, be every mistake 5 light light application time+0.5 hour, by that analogy ... light application time remains unchanged when being increased to 13 hours every days, and intensity of illumination is controlled at 50 Lx～100 Lx; Parent population goes into Chi Houxian and cultivated 2 days under 10 ℃ of water temperatures, played intensification on the 3rd day, from day counting of intensification, water temperature is initial water temperature+1 ℃ after 7 days, and water temperature is initial water temperature+1 ℃+1 ℃ after 14 days, water temperature is initial water temperature+1 ℃+1 ℃+1 ℃ after 21 days, be 7 days water temperature+1 of every mistake ℃, by that analogy ..., kept 10 when waiting to rise to 14 ℃, 7 days water temperature+1 of every mistake ℃ keep constant temperature when going straight up to 21 ℃ again; The ice fresh fish of throwing something and feeding adds vitamin E, day throws something and feeds 1～2 time, and feeding rate control in 3%～5% of fish body weight, morning or throw something and feed at dusk, day quantity of exchanged water 100%.
2, gonad maturity accelerating and hastening parturition
Water temperature rises to 14 ℃, the parent population sexual gland germinates, water temperature rises to 20 ℃, from pectoral fin base portion or dorsal fin base portion injection ocyodinic, ocyodinic LHRH-A hormone, the raun injected dose: per kilogram fish body weight LHRH-A consumption 600 μ g, the injected dose of milter reduces by half, and the LHRH-A hormone is made suspension with vitamin C and is injected.The illuminance of environment of hastening parturition should remain on 100Lx, and the ecbolic effect time is 30 hours, waits to lay eggs and draws in the net to collect fertilized egg after finishing.
Two, with the pond seed rearing
1, the same pond hatching of spotted maigre and large yellow Crocker fertilized egg
Will be through washing, sterilize and removing the equivalent amount of bad ovum or the spotted maigre of weight and the fertilized egg of large yellow Crocker and put into same nursery pond, the water temperature of control nursery pond is 20 ℃ ± 0.5 ℃, and salinity is 23 ‰～30 ‰, and total hatching density is 15～200,000/m
3, in the hatching process, sinkings of turning white of dead ovum, therefore, the dead ovum sucking-off bottom should sinking to every day, and add 20% new water, in case water quality degenerates.To inflate in right amount in the hatching process, but when soil pick-up, to stop the inflation, treat that prelarva hatches after, stop the supple of gas or steam, dead ovum is removed in siphon;
2, prelarva in earlier stage
The water temperature of nursery pond is 21.5 ℃～22.5 ℃, the spotted maigre that hatches and large yellow Crocker prelarva is adopted add water planting and educate, and cultivating density is 10 ~ 150,000 tails/m
3, adding water every day is 10% ~ 20% of Chi Shui, till filling it up with, and inflation, prelarva is the endogenous trophophase in earlier stage, need not to throw something and feed;
3, the prelarva later stage
The water temperature of nursery pond is 22.5 ℃～23.5 ℃, the 3rd day the prelarva in hatching back, the yolk sac absorption finishes but still has oily ball, begins to ingest, the 7th day prelarva in hatching back, phototaxis is strong, and the swimming ability is strong, and oily ball absorbs and finishes, rely on the existence of external source food fully, enter the prelarva later stage, yolk sac and oily ball absorb to each fin fin ray growth complete, and body length is less than 10mm; Change water twice every day, each quantity of exchanged water is 30% ~ 50% of Chi Shui, and cultivating density is 8 ~ 100,000 tails/m
3, every day soil pick-up once, the prelarva later stage large yellow Crocker very fast than spotted maigre growth, the wheel animalcule of strengthening through nutrition so throw something and feed earlier when throwing something and feeding 20 days, began in the 12nd day the to throw something and feed artemia nauplii strengthened through nutrition 4 days began the copepoda of throwing something and feeding on the 12nd day, began the domestication manufactured feed of throwing something and feeding on the 15th day;
4, the postlarva phase
The water temperature of nursery pond is 23.5 ℃～24.5 ℃, postlarva during the phase each fin fin ray grow and completely form to squamation, the long 10.0 ~ 20.0mm of body changes water twice every day, each quantity of exchanged water is 50% of Chi Shui, inflation; Cultivate density 5 ~ 80,000 tails/m
3, every day, soil pick-up twice, mainly based on the manufactured feed of throwing something and feeding, the unsuitable sunlight direct projection of postlarva stage and night light-illuminating, in order to avoid cluster anoxic or accuse each other;
5, regulating and controlling water quality
According to life habit and the occurrence regularity of spotted maigre and large yellow Crocker, note adjusting quantity of exchanged water in good time, keep good water quality environment, reduce morbidity risk rate; Cultivate the later stage along with water temperature raises, daily ration, feeding quantity increases, and damage by disease and insect very easily takes place in the metabolite accumulation.Wherein mainly based on the prevention ichthyophthirius, in cultivating process, use fresh chlorella, keep density 40 * 10
4Individual/ml carries out regulating and controlling water quality.
Three, seed separates
When the spotted maigre in the nursery pond and large yellow Crocker grow to total length 20～30mm the time, can carry out seed as required separates, the method of separating is siphonage, namely above nursery pond, hide with black cloth, one jiao of openning of reserving one 1 square metre at nursery pond, utilize the phototaxis of large yellow Crocker seed, with the plastic flexible pipe of diameter 5～8cm the large yellow Crocker juvenile fish siphon on upper strata is separated.
In same nursery pond, the space layout of the juvenile fish of spotted maigre and two kinds of fish of large yellow Crocker complementation in water body, improved the output of seedling of bait utilization and unit of water body, quantity of exchanged water and the abundance of food have been reduced, human and material resources and financial resources have been saved, the green production theory that meets current energy-saving low-carbon has very important meaning to the sustainable development that promotes spotted maigre and large yellow Crocker aquaculture.
Specific embodiment
The invention provides a kind of indoor mixing seedling-cultivating method of spotted maigre and large yellow Crocker, this cultural method mainly hand operated from following technology.
One, parent population branch pond is cultivated accelerating and is hastened parturition
(1) the parent population accelerating of spotted maigre
1, the cultivation of parent population
Preceding 70 days of artificial induced spawning is selected the spotted maigre of physique stalwartness, moves into the culture pond of 1.5 meters of the depth of waters by male and female respectively than 2:1, and density is 1.5kg/m
3, the husky filter of seawater, per 4～5m
2Water body is put 1 gas charging stone; With around the parent fish rearing pond and top with the black cloth shading, 2 60 W fluorescent lamps are installed at 1 meter above 1.5 meters of every cultivation pond depth of waters, initial day illumination is 8 hours, it is initial day illumination time+0.5 hour after 5 days, it is initial day illumination time+0.5 hour+0.5 hour after 10 days, it is initial day illumination time+0.5 hour+0.5 hour+0.5 hour after 15 days, be every mistake 5 light light application time+0.5 hour, by that analogy ... when the same day, light application time reached 13 hours every days, keep day 13 hours every days of illumination time constant, intensity of illumination is in 50 Lx～100 Lx scopes; Parent population goes into Chi Houxian and cultivated 2 days under 10 ℃ of water temperatures, played intensification on the 3rd day, from day counting of intensification, water temperature is initial water temperature+1 ℃ after 6 days, and water temperature is initial water temperature+1 ℃+1 ℃ after 12 days, water temperature is initial water temperature+1 ℃+1 ℃+1 ℃ after 18 days, be 6 days water temperature+1 of every mistake ℃, by that analogy ..., kept 7 when waiting to rise to 14 ℃, 6 days water temperature+1 of every mistake ℃ keep constant temperature when going straight up to 22 ℃～23 ℃ again; Bait adds vitamin E for the ice fresh fish, and day throws something and feeds 1～2 time, and morning or dusk throw something and feed, and feeding rate is controlled in 3%～5% of fish body weight, and a day quantity of exchanged water is 100%.
2, gonad maturity accelerating and hastening parturition
Water temperature rises to 20 ℃, from pectoral fin base portion or dorsal fin base portion injection hormone, the ocyodinic hormotone of LHRH-A and HCG, the raun injected dose: per kilogram fish body weight LHRH-A consumption 14 μ g add HCG consumption 130 IU, the injected dose of milter all reduces by half, and hormotone is made suspension with vitamin C and injected; The illuminance of environment of hastening parturition should remain on 100Lx, and the ecbolic effect time is 35 hours, waits to lay eggs and draws in the net to collect fertilized egg after finishing.
(2) the parent population accelerating of large yellow Crocker
1, the cultivation of parent population
Preceding 50 days of artificial induced spawning is selected the spotted maigre of physique stalwartness, moves into the culture pond of 1.5 meters of the depth of waters by male and female respectively than 2:1, and density is 1.5kg/m
3, the husky filter of seawater, per 4～5m
2Water body is put 1 gas stone; With around the parent fish rearing pond and top with the black cloth shading, 2 60 W fluorescent lamps are installed at 1 meter above 1.5 meters of every cultivation pond depth of waters, initial day illumination is 8 hours, it is initial day illumination time+0.5 hour after 5 days, it is initial day illumination time+0.5 hour+0.5 hour after 10 days, it is initial day illumination time+0.5 hour+0.5 hour+0.5 hour after 15 days, be every mistake 5 light light application time+0.5 hour, by that analogy ... light application time remains unchanged when being increased to 13 hours every days, and intensity of illumination is in 50 Lx～100 Lx scopes; Parent population goes into Chi Houxian and cultivated 2 days under 10 ℃ of water temperatures, played intensification on the 3rd day, from day counting of intensification, water temperature is initial water temperature+1 ℃ after 7 days, and water temperature is initial water temperature+1 ℃+1 ℃ after 14 days, water temperature is initial water temperature+1 ℃+1 ℃+1 ℃ after 21 days, be 7 days water temperature+1 of every mistake ℃, by that analogy ..., kept 10 when waiting to rise to 14 ℃, 7 days water temperature+1 of every mistake ℃ keep constant temperature when going straight up to 21 ℃ again; The ice fresh fish of throwing something and feeding adds vitamin E, day throws something and feeds 1～2 time, and feeding rate control in 3%～5% of fish body weight, morning or throw something and feed at dusk, day quantity of exchanged water 100%.
2, gonad maturity accelerating and hastening parturition
Water temperature rises to 20 ℃, from pectoral fin base portion or dorsal fin base portion injection ocyodinic, and ocyodinic LHRH-A hormone, the raun injected dose: per kilogram fish body weight LHRH-A consumption 600 μ g, the injected dose of milter reduces by half; The LHRH-A hormone is made suspension with vitamin C and is injected.The illuminance of environment of hastening parturition should remain on 100Lx, and the ecbolic effect time is 30 hours, waits to lay eggs and draws in the net to collect fertilized egg after finishing.
Two, with the pond seed rearing
1, the same pond hatching of spotted maigre and large yellow Crocker fertilized egg
The nursery pond area is 30m
2, the dark 1.8m in pond, depth of water 1m during hatching, the control temperature of fry nursing pool is 20 ℃ ± 0.5 ℃, and salinity is 23 ‰～30 ‰, puts into spotted maigre and the large yellow Crocker fertilized egg of equivalent amount or weight respectively, and total hatching density is 10～150,000/m
3, should be in time in the hatching process will sink to the dead ovum sucking-off of bottom, and add 20% new water and inflation, but when soil pick-up, will stop inflation, treat that prelarva hatches after, stop the supple of gas or steam, dead ovum is removed in siphon, the inclination incubator box allows prelarva enter in the pond naturally.
2, prelarva in earlier stage
The water temperature of nursery pond is 22.5 ℃～23.5 ℃, the spotted maigre of hatching and large yellow Crocker prelarva is adopted add water planting and educate, and cultivation density is 100,000 tails/m
3, adding water every day is 10% ~ 20% of Chi Shui, adds to till the depth of water 1.5m, inflation need not to throw something and feed.
3, the prelarva later stage
The water temperature of nursery pond is 22.5 ℃～23.5 ℃, and the 3rd day the prelarva in hatching back begins to ingest, and the 7th day young fish length in hatching back changes water twice every day less than 10mm, and each quantity of exchanged water is 30% ~ 50% of Chi Shui, and density 8 ~ 100,000 tails/m is cultivated in inflation
3Every day soil pick-up once, throw something and feed earlier when the throwing something and feeding wheel animalcule of strengthening through nutrition 20 days, density is 10-25/ml, began in the 12nd day the to throw something and feed artemia nauplii strengthened through nutrition 4 days, density is 1-2/ml, begins the copepoda of throwing something and feeding on the 12nd day, density is 1-2/ml, begins the domestication manufactured feed of throwing something and feeding on the 15th day.
4, the postlarva phase
The water temperature of nursery pond is 23.5 ℃～24.5 ℃, and the long 10.0 ~ 20.0mm of body changes water twice every day, and each quantity of exchanged water is 50% of Chi Shui, and density 5 ~ 80,000 tails/m is cultivated in inflation
3, every day, soil pick-up twice, based on the manufactured feed of throwing something and feeding.
5, regulating and controlling water quality
Use fresh chlorella in cultivating process, keeping density is 40 * 10
4Individual/ml.
Three, seed separates
When the spotted maigre in the nursery pond and large yellow Crocker grow to total length 20～30mm the time, above nursery pond, hide with black cloth, reserve one 1 square metre openning, utilize the phototaxis of large yellow Crocker seed, with the plastic flexible pipe employing siphon method of diameter 5～8cm the large yellow Crocker on upper strata is separated.
Four, cultivate the result
At 30m
2Cement pit in put into the fertilized egg of 1.5kg spotted maigre, quantity is 2,200,000 ovum, incubation rate is 85.0%, obtains newly hatched larvae 1,870,000 tails, through cultivating, finally obtains 2～3 centimetres about 24.31 ten thousand tails of juvenile fish, incubation rate is 13.0%; In the large yellow Crocker fertilized egg that Tong Chizhong puts into 1.5kg, incubation rate is 90.0%, obtains newly hatched larvae 1,620,000 tails, through cultivating, finally obtains 2-3 centimetre juvenile fish 45.36 ten thousand tails, and incubation rate is 28.0%.In other words, at 30m
2Cement pit in carry out spotted maigre and large yellow Crocker mixing grow seedlings, total output of seedling of two kinds of fish reaches 69.67 ten thousand tails, the unit are incubation rate reaches 2300 tails/m
2, improved the availability of nursery pond greatly, reduced seedling cost.
Claims (1)
1. a spotted maigre and large yellow Crocker mix the method for growing seedlings, and it is characterized in that this mixings seedling-cultivating method comprises: the branch pond cultivation accelerating of (I), spotted maigre and large yellow Crocker parent population and hastening parturition; Cultivate in the same pond of (II), spotted maigre and large yellow Crocker seed; (III), seed separate; Wherein:
The branch pond of (I) described spotted maigre and large yellow Crocker parent population is cultivated accelerating and is hastened parturition, and follows these steps to operation:
One, parent population branch pond is cultivated accelerating and is hastened parturition
(1) the parent population accelerating of spotted maigre
(1), the cultivation of parent population
Preceding 70 days of artificial induced spawning is selected the spotted maigre of physique stalwartness, moves into the culture pond of 1.5 meters of the depth of waters by male and female respectively than 2:1, and density is 1.5kg/m
3, the husky filter of seawater, per 4～5m
2Water body is put 1 gas charging stone; With around the parent fish rearing pond and top with the black cloth shading, 2 60 W fluorescent lamps are installed at 1 meter above 1.5 meters of every cultivation pond depth of waters, initial day illumination is 8 hours, it is initial day illumination time+0.5 hour after 5 days, it is initial day illumination time+0.5 hour+0.5 hour after 10 days, it is initial day illumination time+0.5 hour+0.5 hour+0.5 hour after 15 days, be every mistake 5 light light application time+0.5 hour, by that analogy ... when the same day, light application time reached 13 hours every days, keep day 13 hours every days of illumination time constant, intensity of illumination is in 50 Lx～100 Lx scopes; Parent population goes into Chi Houxian and cultivated 2 days under 10 ℃ of water temperatures, played intensification on the 3rd day, from day counting of intensification, water temperature is initial water temperature+1 ℃ after 6 days, and water temperature is initial water temperature+1 ℃+1 ℃ after 12 days, water temperature is initial water temperature+1 ℃+1 ℃+1 ℃ after 18 days, be 6 days water temperature+1 of every mistake ℃, by that analogy ..., kept 7 when waiting to rise to 14 ℃, 6 days water temperature+1 of every mistake ℃ keep constant temperature when going straight up to 22 ℃～23 ℃ again; Bait adds vitamin E for the ice fresh fish, and day throws something and feeds 1～2 time, and morning or dusk throw something and feed, and feeding rate is controlled in 3%～5% of fish body weight, and a day quantity of exchanged water is 100%;
(2), gonad maturity accelerating and hastening parturition
Water temperature rises to 20 ℃, from pectoral fin base portion or dorsal fin base portion injection hormone, the ocyodinic hormotone of LHRH-A and HCG, the raun injected dose: per kilogram fish body weight LHRH-A consumption 14 μ g add HCG consumption 130 IU, the injected dose of milter all reduces by half, and hormotone is made suspension with vitamin C and injected; The illuminance of environment of hastening parturition should remain on 100Lx, and the ecbolic effect time is 35 hours, waits to lay eggs and draws in the net to collect fertilized egg after finishing;
(2) the parent population accelerating of large yellow Crocker
(1), the cultivation of parent population
Preceding 50 days of artificial induced spawning is selected the large yellow Crocker of physique stalwartness, moves into the culture pond of 1.5 meters of the depth of waters by male and female respectively than 2:1, and density is 1.5kg/m
3, the husky filter of seawater, per 4～5m
2Water body is put 1 gas stone; With around the parent fish rearing pond and top with the black cloth shading, 2 60 W fluorescent lamps are installed at 1 meter above 1.5 meters of every cultivation pond depth of waters, initial day illumination is 8 hours, it is initial day illumination time+0.5 hour after 5 days, it is initial day illumination time+0.5 hour+0.5 hour after 10 days, it is initial day illumination time+0.5 hour+0.5 hour+0.5 hour after 15 days, be every mistake 5 light light application time+0.5 hour, by that analogy ... light application time remains unchanged when being increased to 13 hours every days, and intensity of illumination is in 50 Lx～100 Lx scopes; Parent population goes into Chi Houxian and cultivated 2 days under 10 ℃ of water temperatures, played intensification on the 3rd day, from day counting of intensification, water temperature is initial water temperature+1 ℃ after 7 days, and water temperature is initial water temperature+1 ℃+1 ℃ after 14 days, water temperature is initial water temperature+1 ℃+1 ℃+1 ℃ after 21 days, be 7 days water temperature+1 of every mistake ℃, by that analogy ..., kept 10 when waiting to rise to 14 ℃, 7 days water temperature+1 of every mistake ℃ keep constant temperature when going straight up to 21 ℃ again; The ice fresh fish of throwing something and feeding adds vitamin E, day throws something and feeds 1～2 time, and feeding rate control in 3%～5% of fish body weight, morning or throw something and feed at dusk, day quantity of exchanged water 100%;
(2), gonad maturity accelerating and hastening parturition
Water temperature rises to 20 ℃, from pectoral fin base portion or dorsal fin base portion injection ocyodinic, and ocyodinic LHRH-A hormone, the raun injected dose: per kilogram fish body weight LHRH-A consumption 600 μ g, the injected dose of milter reduces by half; The LHRH-A hormone is made suspension with vitamin C and is injected.The illuminance of environment of hastening parturition should remain on 100Lx, and the ecbolic effect time is 30 hours, waits to lay eggs and draws in the net to collect fertilized egg after finishing;
Cultivate in the same pond of (II) described spotted maigre and large yellow Crocker seed, follows these steps to operation:
(1), the same pond hatching of spotted maigre and large yellow Crocker fertilized egg
The nursery pond area is 30m
2, the dark 1.8m in pond, depth of water 1m during hatching, the control temperature of fry nursing pool is 20 ℃ ± 0.5 ℃, and salinity is 23 ‰～30 ‰, puts into spotted maigre and the large yellow Crocker fertilized egg of equivalent amount or weight respectively, and total hatching density is 10～150,000/m
3, should in time will sink to the dead ovum sucking-off of bottom in the hatching process, and add 20% new water and inflation, but when soil pick-up, will stop inflation; After treating that prelarva hatches, stop the supple of gas or steam, dead ovum is removed in siphon, and the inclination incubator box allows prelarva enter in the pond naturally;
(2), prelarva in earlier stage
The water temperature of control nursery pond is 22.5 ℃～23.5 ℃, the spotted maigre of hatching and large yellow Crocker prelarva is adopted add water planting and educate, and cultivation density is 100,000 tails/m
3, adding water every day is 10%～20% of Chi Shui, adds to till the depth of water 1.5m, inflation need not to throw something and feed;
(3), the prelarva later stage
The water temperature of control nursery pond is 22.5 ℃～23.5 ℃, and the 3rd day the prelarva in hatching back begins to ingest, and the 7th day young fish length in hatching back changes water twice every day less than 10mm, and each quantity of exchanged water is 30%～50% of Chi Shui, and density 8～100,000 tails/m is cultivated in inflation
3Every day soil pick-up once, throw something and feed earlier when the throwing something and feeding wheel animalcule of strengthening through nutrition 20 days, density is 10-25/ml, began in the 12nd day the to throw something and feed artemia nauplii strengthened through nutrition 4 days, density is 1-2/ml, begins the copepoda of throwing something and feeding on the 12nd day, density is 1～2/ml, begins the domestication manufactured feed of throwing something and feeding on the 15th day;
(4), the postlarva phase
The water temperature of control nursery pond is 23.5 ℃～24.5 ℃, and the long 10.0～20.0mm of body changes water twice every day, and each quantity of exchanged water is 50% of Chi Shui, and density 5～80,000 tails/m is cultivated in inflation
3, every day, soil pick-up twice, based on the manufactured feed of throwing something and feeding;
(5), regulating and controlling water quality
Use fresh chlorella in cultivating process, keeping density is 40 * 10
4Individual/ml;
(III) described seed separates, and follows these steps to operation:
When the spotted maigre in the nursery pond and large yellow Crocker grow to total length 20～30mm the time, above nursery pond, hide with black cloth, reserve one 1 square metre openning, utilize the phototaxis of large yellow Crocker seed, with the plastic flexible pipe employing siphon method of diameter 5～8cm the large yellow Crocker on upper strata is separated.
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| CN102613120A (en) * | 2012-04-16 | 2012-08-01 | 浙江省海洋水产研究所 | Acquiring method for high-quality germ cells of spotted maigre |
| CN102870719B (en) * | 2012-10-29 | 2014-06-18 | 浙江省海洋水产研究所 | Artificial induced spawning, fertilization and family establishing method of spotted maigre |
| CN104054597B (en) * | 2014-03-17 | 2016-01-20 | 浙江省海洋水产研究所 | A kind of spotted maigre fingerlings artificial manufactured feed domesticating method |
| CN104126535B (en) * | 2014-08-18 | 2016-03-09 | 海南大学 | The artificial fecundation method of a kind of ripple lip fish |
| CN104304117B (en) * | 2014-10-22 | 2017-01-11 | 浙江海洋学院 | Hatched fry separating device |
| CN105594638A (en) * | 2015-12-17 | 2016-05-25 | 中国水产科学研究院东海水产研究所 | Method for obtaining illumination intensity suitable for growth and development of juvenile pomfret |
| CN106172117B (en) * | 2016-07-14 | 2019-08-06 | 浙江省海洋水产研究所 | Little yellow croaker raun and Larimichthys crocea milter cross breeding method |
| CN115474563B (en) * | 2022-09-02 | 2023-06-06 | 中国水产科学研究院东海水产研究所 | Cultivation method for improving anti-current capability of fry of large yellow croaker |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101283677A (en) * | 2008-06-04 | 2008-10-15 | 厦门大学 | Artificial insemination process of large yellow croaker |
| CN102124978A (en) * | 2011-04-10 | 2011-07-20 | 浙江海洋学院 | Large yellow croaker breeding method |
| CN102124974A (en) * | 2011-03-15 | 2011-07-20 | 浙江省海洋水产研究所 | Method for artificially breeding seed of spotted maigre |
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| CN101283677A (en) * | 2008-06-04 | 2008-10-15 | 厦门大学 | Artificial insemination process of large yellow croaker |
| CN102124974A (en) * | 2011-03-15 | 2011-07-20 | 浙江省海洋水产研究所 | Method for artificially breeding seed of spotted maigre |
| CN102124978A (en) * | 2011-04-10 | 2011-07-20 | 浙江海洋学院 | Large yellow croaker breeding method |
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| Title |
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| 楼宝，史会来，毛国民."黄姑鱼全人工繁育及大规格苗种培育技术研究".《现代渔业信息》.2011,第26卷(第3期),20-23页. |
| 楼宝,史会来,毛国民."黄姑鱼全人工繁育及大规格苗种培育技术研究".《现代渔业信息》.2011,第26卷(第3期),20-23页. * |
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