CN102870719B - Artificial induced spawning, fertilization and family establishing method of spotted maigre - Google Patents
Artificial induced spawning, fertilization and family establishing method of spotted maigre Download PDFInfo
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- CN102870719B CN102870719B CN201210420294.4A CN201210420294A CN102870719B CN 102870719 B CN102870719 B CN 102870719B CN 201210420294 A CN201210420294 A CN 201210420294A CN 102870719 B CN102870719 B CN 102870719B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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Abstract
An artificial induced spawning, fertilization and family establishing method of spotted maigre belongs to the technical field of fish culture. The method comprises the steps of 1, culturing parent fish and enhancing nutrition; 2, selecting the patent fish subjected to induced spawning; 3, selecting odinagogue and dosage; 4, selecting the induced spawning time and the induced spawning temperature; and 5, conducting artificial fertilization and establishing the family. According to the method, based on the gonad development law of the spotted maigre, the optimal induced spawning period of the patent fish and the optimal odinagogue are determined, the relationship between the culture temperature and the induced spawning effect time of the patent fish subjected to induced spawning is established, the success rate of the induced spawning is high, the spawning time is unified, the spawning quantity is high, the spawn supply to the patent fish according to needs is realized.
Description
Technical field
The present invention relates to the cultural technique field of fish, be specifically related to artificial induced spawning fertilization and the method for establishing stemma of a kind of spotted maigre.
Background technology
Spotted maigre (Nibea albiflora), is commonly called as yellow mother-in-law chicken, belongs to Perciformes, Sciaenidae, Nibea, lower water layer fish in coastal waters, for the important propagation of China's southeastern coast and breed variety, there is higher nutrition and medical value, market demand is in continuous rising.The at present breeding of spotted maigre relies on parent's physical maturity to realize to lay eggs mostly, due to the difference of spotted maigre parent maturity, cause the asynchronism of laying eggs, cannot realize the manual control to egg-laying time and egg laying amount, both be unfavorable for producing, also cannot have met the requirement of fish genetic breeding directional cross.The problem that the ovulation that the fish parental population maturity of fish gonads differences such as spotted maigre that solved artificial induced spawning to a certain extent cause is not concentrated, ovulatory cycle is grown, by artificial induced spawning, can impel that parent population is disposable lays eggs, and lays eggs many, emerges neat.As Chinese invention patent application " spotted maigre does sth. in advance mating system ", disclosure of the invention 102257978A, the mating system ahead of time of a kind of spotted maigre is disclosed, step comprises: select the above male parent population of 400g and the above female parent population of 600g, press 1:1 in culture pond, every cubic metre of water body is put 2 ~ 5 pairs of male and female parent populations in a suitable place to breed; Controlled light time and water temperature: in January, light application time was extended down to 14 hours/day from 10 hours/day, and intensity of illumination is at 300-500lux; Water temperature is from 10 DEG C of beginnings, and 1 DEG C/day rises to 18 DEG C ~ 19 DEG C and stablizes seven days; Then 0.5 DEG C ~ 1 DEG C/day rise to 22 DEG C stop heat up; Maintain 14 hours/day, 22 DEG C one month, obtain the male and female parent population of gonad maturity; Female parent population is hastened parturition by 0.3-1.5 microgram/kilogram dosage injection with hormone, male reducing by half; Parent population natural insemination under hormone induction obtains fertilized egg, then carries out conventional artificial breeding.But the method is not determined the best of parent hasten parturition phase and ocyodinic in detail, does not also set up clear and definite hasten parturition parents culture temperature and the relation of ecbolic effect time, cannot form the long-term superior families of a spotted maigre system.
Summary of the invention
For problems of the prior art, the present invention is on the basis of spotted maigre gonad development rule, and the best of determining parent hasten parturition phase and ocyodinic, set up parents culture temperature and the relation of ecbolic effect time of hastening parturition.
In order to solve the problems of the technologies described above, the object of the present invention is to provide artificial induced spawning fertilization and the method for establishing stemma of a kind of spotted maigre, it is characterized in that according to the following steps:
(1) spotted maigre parent is moved in indoor booth and cultivated, in breeding process, control water temperature between 14～19 DEG C, more than dissolved oxygen 5.0mg/L, cultivation density 10～15 tail/cubic meters, daily throwing something and feeding in material, added every 2～5 days the chilled small fish of throwing something and feeding once, feeding volume is controlled at quality of fishes 2～3%, and described chilled small fish is taking hairtail and little yellow croaker as main;
(2) the spotted maigre parent who chooses is proceeded to indoor cement pit and carry out fortification, during cultivation, control cultivation density 5～10 tail/cubic meters, adopt sand filter seawater, change water every morning more than 90%, 24h oxygenation, more than keeping Dissolved Oxygen in Water 5.0mg/L, daily throwing something and feeding in material, add the razor clam of hanging of throwing something and feeding 1 time every day, feeding volume is quality of fishes 4～7%, and adds 0.2% vitamin E in the razor clam of hanging of throwing something and feeding;
(3) select belly and have protuberance, more soft with have gentle hands light touches belly, cloacal aperture has slight projection and the reddish parent of color as parent population to be hastened parturition, be the parent of gonadal maturation to the IV phase, with injection luteinising hormone-releasing hormo as ocyodinic, adopt double injection method to carry out lumbar injection to spotted maigre raun, while hastening parturition for the first time, injected dose is that 1kg parent injects 1 μ g oxytocins, while injection for the second time, injected dose is that 1kg parent injects 1.5-2 μ g oxytocins, adopt shot method to carry out lumbar injection to spotted maigre milter, injected dose is that 1kg parent injects 1 μ g oxytocins,
(4) after parent population has been injected oxytocins, immediately they are put into respectively in different spawning inducing pool and are cultivated, the cultivation pond of raun covers Chi Kou with black cloth, after between equivalence is seasonable, to the milter semen collection after hastening parturition, rinse the cloacal aperture of milter with fresh water, clean, push gently belly, with suction pipe by seminal fluid collecting to temporary in centrifuge tube, again the raun after hastening parturition is got to ovum, rinse equally the cloacal aperture of raun with fresh water, clean, push gently belly, use plastic cup recovery of ova, by seminal fluid Hank ' the s liquid of having collected, according to 1:30 dilution proportion, then add the ratio of 10ml seminal fluid to mix with ovum according to every 100g ovum, and mix immediately, then add seawater to complete fertilization, described Hank ' s formula of liquid is: NaCl 8.01 g/l, KCl 0.4 g/l, CaCl
20.14 g/l, NaHCO
30.35 g/l, KH2PO
40.06 g/l, Glucose 0.34 g/l, pH 8.0,
(5) while collecting seminal fluid and ovum, respectively the ovum of the seminal fluid of each milter and raun is all preserved separately, observe respectively under the microscope, choose milter that sperm viability is good and the complete rule of ovum shape and transparent raun and carry out 1 pair of 1 artificial insemination, and the clip tail fin low temperature preservation respectively of female and male parent population, and make a record, for setting up family.
Artificial induced spawning fertilization and the method for establishing stemma of described a kind of spotted maigre, it is characterized in that in step (1) daily throwing something and feeding in material in the time that water temperature is more than 16 DEG C, added the chilled small fish of throwing something and feeding once every 2～3 days, feeding volume is controlled at quality of fishes 2～3%, when water temperature daily throwing something and feeding in material during lower than 16 DEG C, added every 3～5 days the chilled small fish of throwing something and feeding once, feeding volume is controlled at quality of fishes 2～3%.
Artificial induced spawning fertilization and the method for establishing stemma of described a kind of spotted maigre, it is characterized in that in step (2) at the surrounding of cement pit and top with black cloth shading, and 1m installs the fluorescent lamp of 2 60W up, make light be evenly distributed at the water surface of cultivating pond as far as possible, beginning Continuous irradiation every day 8 hours, and increased by 1 hour every 5 days, irradiate 14 hours to every day, after maintain 14 hours/day, intensity of illumination is 50～100Lx.
The artificial induced spawning fertilization of described a kind of spotted maigre and method for establishing stemma, is characterized in that the described injection luteinising hormone-releasing hormo of step (3) selects injection Luteinizing hormone releasing-hormone A2.
The artificial induced spawning fertilization of described a kind of spotted maigre and method for establishing stemma, is characterized in that the inject time for the second time of raun that step (3) is described and all will be selected in the 8:00 in morning the inject time of milter.
The artificial induced spawning fertilization of described a kind of spotted maigre and method for establishing stemma, is characterized in that the described effect time of step (4) is for be 31-32h when cultivating water temperature while being 20 DEG C the ecbolic effect time, and in the time that cultivation temperature is 22 DEG C, the ecbolic effect time is 29-30h.
Artificial induced spawning fertilization and the method for establishing stemma of described a kind of spotted maigre, what it is characterized in that described 1 couple of step (5) 1 artificial insemination employing is dry method fertilization.
Artificial induced spawning fertilization and the method for establishing stemma of a kind of spotted maigre of the present invention, the best of determining in detail parent hasten parturition phase and ocyodinic, set up clear and definite hasten parturition parents culture temperature and the relation of ecbolic effect time, and the success rate of hastening parturition is high, egg-laying time is unified, and egg laying amount is many, has solved spotted maigre nonsynchronous problem of ovulating, realize to parent supply as required ovum, be also convenient to set up the long-term superior families of spotted maigre system.
Embodiment
Below in conjunction with specific embodiment, the invention will be further described.
Embodiment: cultivate in spotted maigre parent is moved into indoor booth by late November, at the bottom of pool wall and pond, all renovate with cement, the cultivation depth of water is 1.5-2.0m, cultivation density 10～15 tail/cubic meters, control water temperature between 14～19 DEG C, adjust feeding volume according to the temperature of water body, in the time that water temperature is more than 16 DEG C, daily throwing something and feeding in material, added the chilled small fish of throwing something and feeding once every 2-3 days, taking hairtail and little yellow croaker as main.When water temperature is during lower than 16 DEG C, daily throwing something and feeding in material, added every 3-5 days the chilled small fish of throwing something and feeding once, and feeding volume is controlled at 2～3% of fish body weight, and in indoor booth, places two, aerator, keeps the dissolved oxygen abundance in water body.Be transported to parent population in indoor cement pit and cultivate and fortification mid-March next year, adopt sand filter seawater, change water every morning more than 90%, 24h oxygenation, more than keeping Dissolved Oxygen in Water 5.0mg/L, throw something and feed in material daily, add the razor clam of hanging of throwing something and feeding 1 time every day, feeding volume is 4～7% of fish body weight, and adds 0.2% vitamin E in the razor clam of hanging of throwing something and feeding.Cultivating pond surrounding and top with black cloth shading, and 1m installs the fluorescent lamp of 2 60W above parent fish rearing pond, make light be evenly distributed at the water surface of cultivating pond as far as possible, beginning Continuous irradiation every day 8 hours, and increased by 1 hour every 5 days, irradiate 14 hours to every day, after maintain 14 hours/day, intensity of illumination is 50～100Lx.
Parent, after fortification, to the first tenday period of a month May next year, selects good spotted maigre raun 200 tails of gonad development and milter 100 tails and is transferred to separately and cultivates in spawning inducing pool.While selecting raun, will select individual large, normal, the healthy parent of body colour, the belly of light groping for fish body can clearly be felt the profile of sexual gland, and belly is softer.While selecting milter, wrap the head of fish body with towel, the slightly milky seminal fluid of extruding belly gently flows out.Parent select with transfer process in, can be suitable with MS-222 anesthesia, to reduce stimulation and the injury to parent.
What ocyodinic was selected is injection Luteinizing hormone releasing-hormone A2 that Ningbo the second hormone factory produces, utilizes 0.7% normal saline dilution to hasten parturition and usually carries out lumbar injection.Select double injection method for raun, while hastening parturition for the first time, injected dose is that 1kg parent injects 1 μ g oxytocins, and while injection for the second time, injected dose is that 1kg parent injects 1.5-2 μ g oxytocins.Spotted maigre milter is adopted to shot method, and injected dose is that 1kg parent injects 1 μ g oxytocins.After parent has injected oxytocins, immediately they are put into respectively in different spawning inducing pool and are cultivated, after hastening parturition, raun and milter are separately to cultivate, and the cultivation pond of raun covers Chi Kou with black cloth.In the time that cultivation water temperature is 20 DEG C, parent's the ecbolic effect time is 31-32h, and when cultivate temperature while being 22 DEG C parent's the ecbolic effect time be 29-30h, inject time for the second time of raun and all will be selected in the 8:00 in morning the inject time of milter, parent is laid eggs before and after the 19:00 of second night, close with the spotted maigre natural spawning time.Mid-May is to mid-June, parent 11 batches altogether hastens parturition, hasten parturition altogether raun 120 tails and milter 80 tails, through the cultivation parent population of artificial induced spawning, the success rate of hastening parturition of milter reaches 100%, and the success rate of hastening parturition of raun reaches 60%-80%, has effectively improved the year of parent population for ovum amount, offer altogether nursery and reach 20000ml high quality fertilized egg of scophthatmus, realize economic benefit and can reach more than 300 ten thousand.
Parent after hastening parturition is carried out respectively to the collection of sperm and ovum.First rinsed the cloacal aperture of milter with fresh water, cleaned, pushed gently belly, with suction pipe by seminal fluid collecting in 2ml centrifuge tube, be placed in insulation ice chest temporary.After seminal fluid collecting is complete, then raun is got to ovum, will, by the cloacal aperture wiped clean of raun, prevent seawater pollution equally, extruding belly, uses plastic cup recovery of ova.By seminal fluid Hank ' the s liquid of having collected according to 1:30 dilution proportion, then add the ratio of 10ml seminal fluid to mix with ovum according to every 100g ovum, and mix immediately, then add a small amount of seawater to complete fertilization, described Hank ' s formula of liquid is: NaCl 8.01 g/l; KCl 0.4 g/l; CaCl
20.14 g/l; NaHCO
30.35 g/l; KH2PO
40.06 g/l; Glucose 0.34 g/l; PH 8.0.
While collecting seminal fluid and ovum, respectively the ovum of the seminal fluid of each milter and raun is all preserved separately, observe respectively under the microscope, choose milter that sperm viability is good and the complete rule of ovum shape and transparent raun and carry out 1 pair 1 artificial dry method fertilization, and the clip tail fin low temperature preservation respectively of female and male parent population, and make a record.Altogether the 16000ml ovum after artificial induced spawning is carried out to artificial insemination, fertilization rate reached to 60% in May to June; Build altogether 28 of spotted maigre family full-sibses, the method is specially adapted to the genetic breeding of spotted maigre.
Claims (1)
1. the artificial induced spawning of spotted maigre fertilization and a method for establishing stemma, is characterized in that according to the following steps:
(1) spotted maigre parent is moved in indoor booth and cultivated, in breeding process, control water temperature between 14～19 DEG C, more than dissolved oxygen 5.0mg/L, cultivation density 10～15 tail/cubic meters, daily throwing something and feeding in material in the time that water temperature is more than 16 DEG C, added the chilled small fish of throwing something and feeding once every 2～3 days, feeding volume is controlled at quality of fishes 2～3%, when water temperature daily throwing something and feeding in material during lower than 16 DEG C, added the chilled small fish of throwing something and feeding once every 3～5 days, feeding volume is controlled at quality of fishes 2～3%, and described chilled small fish is taking hairtail and little yellow croaker as main;
(2) the spotted maigre parent who chooses is proceeded to indoor cement pit and carry out fortification, the surrounding of cement pit and top are with black cloth shading, and 1m installs the fluorescent lamp of 2 60W up, make light be evenly distributed at the water surface of cultivating pond as far as possible, beginning Continuous irradiation every day 8 hours, and increased by 1 hour every 5 days, irradiate 14 hours to every day, after maintain 14 hours/day, intensity of illumination is 50～100Lx, during cultivation, control cultivation density 5～10 tail/cubic meters, adopt sand filter seawater, change water every morning more than 90%, 24h oxygenation, more than keeping Dissolved Oxygen in Water 5.0mg/L, daily throwing something and feeding in material, add the razor clam of hanging of throwing something and feeding 1 time every day, feeding volume is quality of fishes 4～7%, and in the razor clam of hanging of throwing something and feeding, add 0.2% vitamin E,
(3) select belly and have protuberance, more soft with have gentle hands light touches belly, cloacal aperture has slight projection and the reddish parent of color as parent population to be hastened parturition, be the parent of gonadal maturation to the IV phase, with injection luteinising hormone-releasing hormo A2 as ocyodinic, adopt double injection method to carry out lumbar injection to spotted maigre raun, while hastening parturition for the first time, injected dose is that 1kg parent injects 1 μ g oxytocins, while injection for the second time, injected dose is that 1kg parent injects 1.5-2 μ g oxytocins, and will be selected in for the second time 8:00 in morning inject time, adopt shot method to carry out lumbar injection to spotted maigre milter, injected dose is that 1kg parent injects 1 μ g oxytocins, be selected in 8:00 in morning inject time,
(4) after parent population has been injected oxytocins, immediately they are put into respectively in different spawning inducing pool and are cultivated, the cultivation pond of raun covers Chi Kou with black cloth, in the time that cultivation water temperature is 20 DEG C, the ecbolic effect time is 31-32h, in the time that cultivation water temperature is 22 DEG C, the ecbolic effect time is 29-30h, after between equivalence is seasonable, to the milter semen collection after hastening parturition, rinse the cloacal aperture of milter with fresh water, clean, push gently belly, with suction pipe by seminal fluid collecting to temporary in centrifuge tube, again the raun after hastening parturition is got to ovum, rinse equally the cloacal aperture of raun with fresh water, clean, push gently belly, use plastic cup recovery of ova, by seminal fluid Hank ' the s liquid of having collected, according to 1:30 dilution proportion, then add the ratio of 10ml seminal fluid to mix with ovum according to every 100g ovum, and mix immediately, then add a small amount of seawater to complete fertilization, described Hank ' s formula of liquid is: NaCl 8.01 g/l, KCl 0.4 g/l, CaCl
20.14 g/l, NaHCO
30.35 g/l, KH
2pO
40.06 g/l, Glucose 0.34 g/l, pH 8.0,
(5) while collecting seminal fluid and ovum, respectively the ovum of the seminal fluid of each milter and raun is all preserved separately, observe respectively under the microscope, choose milter that sperm viability is good and the complete rule of ovum shape and transparent raun and carry out 1 pair 1 artificial dry method fertilization, and the clip tail fin low temperature preservation respectively of female and male parent population, and make a record, for setting up family.
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| CN104839066A (en) * | 2015-06-02 | 2015-08-19 | 威海圣航水产科技有限公司 | Artificial insemination method of greenlings |
| CN105432531A (en) * | 2015-12-30 | 2016-03-30 | 贵州大学 | Efficient Pu'an silver prussian carp propagation and hatching method |
| CN106577382B (en) * | 2016-11-29 | 2019-08-20 | 浙江省海洋水产研究所 | A kind of building of little yellow croaker family and breeding superior families method |
| CN109315335A (en) * | 2018-11-28 | 2019-02-12 | 中国海洋大学 | Efficient fish culture spawning inducing pool |
| CN110622890A (en) * | 2019-10-28 | 2019-12-31 | 宁德市富发水产有限公司 | Method for rapid artificial fertilization of wild stone head fish |
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| JP3571033B2 (en) * | 2002-05-17 | 2004-09-29 | ヤマハニュートレコアクアテック株式会社 | Fish farming methods |
| JP2007074977A (en) * | 2005-09-14 | 2007-03-29 | Technica:Kk | Method for cultivating fish |
| CN101292636B (en) * | 2008-02-14 | 2010-09-29 | 浙江海洋学院海洋与渔业研究所 | Method for cage culture of spotted maigre |
| CN102124974B (en) * | 2011-03-15 | 2013-01-30 | 浙江省海洋水产研究所 | Method for artificially breeding seed of spotted maigre |
| CN102440204B (en) * | 2011-09-30 | 2013-07-10 | 浙江省海洋水产研究所 | Mixed breeding method for spotted maigre and large yellow croaker |
| CN102613120A (en) * | 2012-04-16 | 2012-08-01 | 浙江省海洋水产研究所 | Acquiring method for high-quality germ cells of spotted maigre |
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