CN100370895C - Artificial mature-promoting culturing method for epinephelus lanceolatus parent fish - Google Patents
Artificial mature-promoting culturing method for epinephelus lanceolatus parent fish Download PDFInfo
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- CN100370895C CN100370895C CNB2005100334709A CN200510033470A CN100370895C CN 100370895 C CN100370895 C CN 100370895C CN B2005100334709 A CNB2005100334709 A CN B2005100334709A CN 200510033470 A CN200510033470 A CN 200510033470A CN 100370895 C CN100370895 C CN 100370895C
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- 241000251468 Actinopterygii Species 0.000 title claims abstract description 24
- 241001217936 Epinephelus lanceolatus Species 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title claims abstract description 15
- 238000012258 culturing Methods 0.000 title claims abstract description 8
- 108010011619 6-Phytase Proteins 0.000 claims abstract description 9
- 238000002347 injection Methods 0.000 claims abstract description 9
- 239000007924 injection Substances 0.000 claims abstract description 9
- 229940085127 phytase Drugs 0.000 claims abstract description 9
- 210000005253 yeast cell Anatomy 0.000 claims abstract description 9
- 210000004907 gland Anatomy 0.000 claims abstract description 8
- GCKMFJBGXUYNAG-UHFFFAOYSA-N 17alpha-methyltestosterone Natural products C1CC2=CC(=O)CCC2(C)C2C1C1CCC(C)(O)C1(C)CC2 GCKMFJBGXUYNAG-UHFFFAOYSA-N 0.000 claims abstract description 6
- GCKMFJBGXUYNAG-HLXURNFRSA-N Methyltestosterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@](C)(O)[C@@]1(C)CC2 GCKMFJBGXUYNAG-HLXURNFRSA-N 0.000 claims abstract description 6
- 229960001566 methyltestosterone Drugs 0.000 claims abstract description 6
- 230000000638 stimulation Effects 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 14
- 230000032696 parturition Effects 0.000 claims description 11
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 10
- 239000004568 cement Substances 0.000 claims description 9
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- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 8
- 239000011707 mineral Substances 0.000 claims description 8
- -1 salt compound Chemical class 0.000 claims description 8
- 241000237502 Ostreidae Species 0.000 claims description 7
- 229930003427 Vitamin E Natural products 0.000 claims description 7
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 7
- 210000002149 gonad Anatomy 0.000 claims description 7
- 235000020636 oyster Nutrition 0.000 claims description 7
- 235000019165 vitamin E Nutrition 0.000 claims description 7
- 229940046009 vitamin E Drugs 0.000 claims description 7
- 239000011709 vitamin E Substances 0.000 claims description 7
- 230000001939 inductive effect Effects 0.000 claims description 6
- 230000001568 sexual effect Effects 0.000 claims description 6
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 5
- 229920002472 Starch Polymers 0.000 claims description 5
- 229930003268 Vitamin C Natural products 0.000 claims description 5
- 235000019698 starch Nutrition 0.000 claims description 5
- 239000008107 starch Substances 0.000 claims description 5
- 235000019154 vitamin C Nutrition 0.000 claims description 5
- 239000011718 vitamin C Substances 0.000 claims description 5
- 210000000006 pectoral fin Anatomy 0.000 claims description 4
- 229930003231 vitamin Natural products 0.000 claims description 4
- 239000011782 vitamin Substances 0.000 claims description 4
- 235000013343 vitamin Nutrition 0.000 claims description 4
- 229940088594 vitamin Drugs 0.000 claims description 4
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 4
- 239000002775 capsule Substances 0.000 claims description 2
- 230000003203 everyday effect Effects 0.000 claims description 2
- 230000036541 health Effects 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 230000001360 synchronised effect Effects 0.000 abstract description 4
- 229910017053 inorganic salt Inorganic materials 0.000 abstract 1
- 239000011833 salt mixture Substances 0.000 abstract 1
- 230000035938 sexual maturation Effects 0.000 abstract 1
- 238000005728 strengthening Methods 0.000 abstract 1
- 235000013601 eggs Nutrition 0.000 description 8
- 241001417495 Serranidae Species 0.000 description 5
- 230000000384 rearing effect Effects 0.000 description 5
- 230000035800 maturation Effects 0.000 description 4
- 102000002322 Egg Proteins Human genes 0.000 description 2
- 108010000912 Egg Proteins Proteins 0.000 description 2
- 241000357439 Epinephelus Species 0.000 description 2
- 241000276618 Perciformes Species 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 210000004681 ovum Anatomy 0.000 description 2
- 210000000582 semen Anatomy 0.000 description 2
- 208000027877 Disorders of Sex Development Diseases 0.000 description 1
- 241000448486 Epinephelinae Species 0.000 description 1
- 241000246500 Gentianella aspera Species 0.000 description 1
- 101800000989 Oxytocin Proteins 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000013270 controlled release Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 201000005611 hermaphroditism Diseases 0.000 description 1
- 230000010196 hermaphroditism Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- XNOPRXBHLZRZKH-DSZYJQQASA-N oxytocin Chemical compound C([C@H]1C(=O)N[C@H](C(N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CSSC[C@H](N)C(=O)N1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(C)C)C(=O)NCC(N)=O)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 XNOPRXBHLZRZKH-DSZYJQQASA-N 0.000 description 1
- 229940030215 pitocin Drugs 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000005070 ripening Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 208000013327 true hermaphroditism Diseases 0.000 description 1
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The present invention relates to an artificial mature-promoting culturing method for epinephelus lanceolatus parent fish. The present invention is characterized in that bait feed is added with yeast cell walls, inorganic salt mixture, phytase, methyltestosterone, etc., the mature-promoting cultivation of male parent fish in sea area net cages and the indoor tank strengthening cultivation are carried out, the mature of parent fish sex glands is promoted by DOM injection and ecological stimulation, and the parent fish naturally spawn. The method of the present invention can realize the manual control of the sex of epinephelus lanceolatus parent fish, the sex controlling success ratio can reach more than 70%, the obtained functional male fish of low age can be applied to the production; more than 50% of the parent fish can reach the synchronous sexual maturation, the natural spawning is realized, a large quantity of gametes of high quality can be obtained, and thereby, the utilization rate and the productive capacity of the parent fish can be improved.
Description
Technical field
The present invention relates to a kind of epinephelus lanceolatus parent fish rearing method, especially artificial mature-promoting culturing method for epinephelus lanceolatus parent fish.
Background technology
Epinephelus lanceolatus fish (Epinephelus lanceolatus) is subordinate to Perciformes, and (Epinephelus (Epinephelus) also claims the rough gentian lithosporic for Perciformes) ， Sushi section (Serranidae), grouper subfamily (Epinephelinae).This is a kind of of build maximum in the ablen, and lithosporic king's title is arranged.
Because grouper is the protogynous fish of hermaphroditism, the process of sexual conversion, advanced age, fish was just changed milter into, and the milter source is difficulty comparatively.Therefore obtaining to induce epinephelus lanceolatus piscinity gland to grow and synchronous mature technology, is the basis of realizing epinephelus lanceolatus artificial propagation and fry rearing.
Inducing epinephelus lanceolatus piscinity gland to grow and mature technology (preparation and the operation technique that comprise the high-effect controlled release hormone system of induced spawning) synchronously both at home and abroad; The screening of the agreeable to the taste bait of seedling (feed), the cultivation of high-quality biological feed, and the kind and the requirement thereof of the bait of definite seedling different developmental phases (or feed); The foundation of seed rearing production model; Aspects such as disease control technology in parent fish rearing and the seedling production process are not seen system's report as yet.Have the research that aspects such as the artificial propagation of other groupers, seed rearing, the conversion of hormone induction sex have been carried out in some countries and regions in the world, but the mature technology that can use is few on producing.The main at present inducing agent for fish, pitocin accelerating that relies on exists epinephelus lanceolatus fish parent fish synchronism maturation undesirable, problems such as the ropy deficiency of gamete (sperm and ovum).
Summary of the invention
The objective of the invention is to develop a kind of cultural method that can make the synchronous preferably maturation of grouper parent population and obtain the high-quality gamete.
The present invention is by adding yeast cell wall in bait, mineral salt compound, phytase, Methyltestosterone etc. carry out the male parent population accelerating of sea area net cage and cultivate and the tank interior reinforced cultivating, carry out the parent population gonad maturity accelerating and hasten parturition by injection DOM and ecological stimulation, obtain a kind of cultural method that can realize the synchronous maturation of epinephelus lanceolatus fish parent fish and obtain a large amount of high-quality gametes, thereby realized purpose of the present invention.
Artificial mature-promoting culturing method for epinephelus lanceolatus parent fish of the present invention is characterized in that comprising the steps:
(1) net cage accelerating in sea area is cultivated: the male parent population that selects physical health is fed separately in net cage, the water temperature of net cage and salinity are controlled in the normal ranges, feed with bait, and in bait, press per kilogram bait and add yeast cell wall 6～8g, mineral salt compound 6～8g, phytase 2～4g, Methyltestosterone 0.5～2mg, vitamin 2～4g and the bonding feed of alphalise starch 10～15g, obtain the functional milter of low age;
(2) tank interior reinforced cultivating: the male and female parent population is moved on to indoor cement pit carry out reinforced cultivating, feed with bait, and in bait, press per kilogram bait and add yeast cell wall 6～8g, mineral salt compound 6～8g, phytase 2～4g, vitamin E capsule 2～4g, the water temperature of cement pit, salinity, pH value and D0 value all are controlled in the conventional scope, the raun belly that obtains obviously expands, and the milter nuptial coloration is obvious;
(3) select belly parent population big, that appear the sexual gland profile and put into the darker spawning inducing pool of light, pectoral fin base portion injection DOM carries out the parent population gonad maturity accelerating and parent population is hastened parturition, the DOM injected dose of parent population gonad maturity accelerating is according to per kilogram parent population 1.0～1.2 μ g, a shot; The DOM injected dose that parent population is hastened parturition is according to per kilogram parent population 6.0～7.0 μ g, and injection at twice accounts for for the first time 1/3 of total amount, accounts for 2/3 of total amount for the second time; Soft to some bellies, after the tangible parent population of sexual gland profile is put into spawning inducing pool, adopt short its natural spawning of ecological stimulation.
Cage size described in the described step (1) is 5m * 3m * 3m, the sea area water temperature range is 13～27 ℃, salinity range is 2.5%～3.0%, 50～60 days cultivation time, can adopt common bait and feeding method, preferably fresh oyster and crab, every day, bait throwing in was twice, feeding rate is 3%～5%, and described vitamin mainly contains vitamin C and vitamin E etc.
Cement pit size described in the described step (2) is 30～50m
3Described bait is fresh oyster and crab, feeding rate is 4%～5%, 25～28 ℃ of water temperature control ranges, the salinity control range is a mass fraction 2.7%～3.1%, pH value control range 7.9～8.5, D0 value control range 6.0～6.8mg/L, day quantity of exchanged water is controlled at 100%～200%, the low light level, inflation.
Ecological stimulation described in the described step (3) is that water temperature is controlled at 25～28 ℃, and salinity is controlled at mass fraction 2.7%～3.1%, and the pH value is controlled at 7.9～8.5, the bath of miniflow water, day quantity of exchanged water is controlled at 100%～200%, the low light level, inflation, and the D0 value is controlled at 6.0～6.8mg/L.
Cultivate by parent population artificial ripening of the present invention, realize other Artificial Control of epinephelus lanceolatus piscinity, sex regulation and control success rate reaches more than 70%, obtains the functional milter of low age and can be applicable to produce; Make the parent population more than 50% reach the synchronism maturation and realize natural spawning, and obtained a large amount of high-quality gametes, thereby improved availability and the production capacity of parent population.
Description of drawings
Fig. 1: the parent population that indoor cement pit is cultivated;
Fig. 2: the raun belly is obvious;
Fig. 3: epinephelus lanceolatus fertilized egg;
Fig. 4: hatch 24 hours epinephelus lanceolatus prelarva;
Embodiment
Following operational instances is further to explanation of the present invention, should not be used as limitation of the present invention.
Embodiment 1
1999～calendar year 2001 from Hainan, Taiwan buys 2～3kg epinephelus lanceolatus of catching in natural sea, totally 627 tails.Through domestication, artificial culture in a few years, by 2004, can select the individuality as parent population was 213 tails, and body weight is 8～63kg.Carry out the sea area net cage of parent population cultivates the fishing row of Tai Pang Wan sea area, Shenzhen Long Qi village industry development Co., Ltd, cage size is 5m * 3m * 3m, year April cultivation time from February, 2004 to 2004, with fresh oyster and crab is bait, day throws twice, feeding rate is 4%, adds yeast cell wall 7g, mineral salt compound 7g, phytase 3g, Methyltestosterone 1.5mg, multivitamin (mainly containing vitamin C, vitamin E) 3g and the bonding feed of alphalise starch 12.5g according to per kilogram bait.The sea area water temperature range is 13～27 ℃, and salinity range is a mass fraction 2.5%～3.0%.
In March, 2004, select the male parent population of 76 tails and in the net cage of sea area, feed (can not squeezing seminal fluid at that time) separately, with fresh oyster and crab is bait, day throws twice, feeding rate is 4%, add yeast cell wall 7g, mineral salt compound 7g, phytase 3g, Methyltestosterone 1.5mg, multivitamin (mainly containing vitamin C, vitamin E) 3g and the bonding feed of alphalise starch 12.5g according to per kilogram bait, carry out the accelerating of male parent population and cultivate.
Move on to 30m to May with parent population in April, 2004
3Indoor cement pit carry out reinforced cultivating, feed with fresh oyster and crab, feeding rate is 4.5%, adds yeast cell wall 7g, mineral salt compound 7g, phytase 3g, multivitamin (mainly containing vitamin C, vitamin E) 3g and the bonding feed of alphalise starch 12.5g according to per kilogram bait.25～28 ℃ of water temperature control ranges, salinity control range are mass fraction 2.7%～3.1%, pH value control range 7.9～8.5, and D0 value control range 6.0～6.8mg/L, a day quantity of exchanged water is controlled at 100%～200%, the low light level, inflation.Impel the male and female parent population further ripe.
Parent population is cultivated through 3～4 years sea area net cages, the average body 17kg that weighs.Male parent population has 57 tails to change male through 2 months tank interior reinforced cultivating, and has seminal fluid to flow out when being squeezed, and changeing male rate is 75%.Raun is cultivated at indoor cement pit, and sexual gland is further ripe, and belly obviously expands, and the milter nuptial coloration is obvious, has reached the requirement that parent population is hastened parturition.
On May 2nd, 2004 carried out the parent population gonad maturity accelerating and parent population is hastened parturition.Male and female parent population gonad maturity accelerating is to inject 1.1 μ g, a shot at pectoral fin base portion injection DOM dosage by the per kilogram parent population; It is pectoral fin base portion injection DOM that parent population is hastened parturition, and dosage is injected 6.5 μ g by the per kilogram parent population, and injection at twice accounts for for the first time 1/3 of total amount, accounts for 2/3 of total amount for the second time.25～28 ℃ of water temperature control ranges, salinity control range are mass fraction 2.7%～3.1%, pH value control range 7.9～8.5, and D0 value control range 6.0～6.8mg/L, a day quantity of exchanged water is controlled at 100%～200%, the low light level, inflation.Epinephelus lanceolatus fish parent fish 82 tails of hastening parturition are hastened parturition after begin about 30h to lay eggs, and milter chases raun.May, 11 morning, 7:00 dragged for ovum, obtained fertilized egg 23.7kg, fertilized egg 18.6kg wherein, and more than 1,500 ten thousand in fertilized egg, fertilization rate is 78.5%.May 11,19:00 began to hatch prelarva (brooding time 24-26h), about 1,070 ten thousand tails of newly hatched larvae, and incubation rate is 71.3%.
-12 days on the 7th June in 2004, epinephelus lanceolatus fish parent fish 76 tails of hastening parturition obtain fertilized egg 21.5kg, fertilized egg 16.2kg wherein, and more than 1,400 ten thousand, fertilization rate is 75.3%, about 7,800,000 tails of newly hatched larvae, and incubation rate is 55.7%.
The developmental state in each stage of parent population and the situation of laying eggs are seen accompanying drawing 1～4.
Claims (3)
1. an artificial mature-promoting culturing method for epinephelus lanceolatus parent fish is characterized in that comprising the steps:
(1) net cage accelerating in sea area is cultivated: the male parent population that selects physical health is fed separately in net cage, 13～27 ℃ of the sea area water temperatures of control net cage, salinity is a mass fraction 2.5%～3.0%, feed with bait, and in bait, press per kilogram bait and add yeast cell wall 6～8g, mineral salt compound 6～8g, phytase 2～4g, Methyltestosterone 0.5～2mg, vitamin 2～4g and the bonding feed of alphalise starch 10～15g, obtain the functional milter of low age;
(2) tank interior reinforced cultivating: the male and female parent population is moved on to indoor cement pit carry out reinforced cultivating, feed with bait, and in bait, press per kilogram bait and add yeast cell wall 6～8g, mineral salt compound 6～8g, phytase 2～4g, vitamin E capsule 2～4g, feeding rate is 4%～5%, 25～28 ℃ of the water temperatures of control cement pit, salinity is a mass fraction 2.7%～3.1%, pH value 7.9～8.5, D0 value 6.0～6.8mg/L, day quantity of exchanged water 100%～200%, the low light level, inflation, the raun belly that obtains obviously expands, and the milter nuptial coloration is obvious;
(3) select the spawning inducing pool that belly is big, parent population that appear the sexual gland profile is put into the low light level, pectoral fin base portion injection DOM carries out the parent population gonad maturity accelerating and parent population is hastened parturition, the DOM injected dose of parent population gonad maturity accelerating is according to per kilogram parent population 1.0～1.2 μ g, a shot; The DOM injected dose that parent population is hastened parturition is according to per kilogram parent population 6.0～7.0 μ g, and injection at twice accounts for for the first time 1/3 of total amount, accounts for 2/3 of total amount for the second time; Soft to some bellies, after the tangible parent population of sexual gland profile is put into spawning inducing pool, adopt short its natural spawning of ecological stimulation, described ecological stimulation is 25～28 ℃ of water temperatures, and salinity is a mass fraction 2.7%～3.1%, pH value 7.9～8.5, the bath of miniflow water, day quantity of exchanged water 100%～200%, the low light level, inflation, D0 value 6.0～6.8mg/L.
2. according to the artificial mature-promoting culturing method for epinephelus lanceolatus parent fish of claim 1, it is characterized in that the cage size described in the step (1) is 5m * 3m * 3m, 50～60 days cultivation time, described bait is fresh oyster and crab, every day, bait throwing in was twice, feeding rate is 3%～5%, and described vitamin contains vitamin C and vitamin E.
3. according to the artificial mature-promoting culturing method for epinephelus lanceolatus parent fish of claim 1 or 2, it is characterized in that the cement pit size described in the step (2) is 30～50m
3, described bait is fresh oyster and crab.
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| CN101933476B (en) * | 2010-08-09 | 2014-03-05 | 广东省大亚湾水产试验中心 | Method for breeding cross-bred 'Hulongban' fish |
| CN103098737B (en) * | 2013-02-26 | 2015-03-11 | 浙江师范大学 | Manpower hasten parturition method of acrossocheilus fasciatus |
| CN103493759B (en) * | 2013-09-12 | 2015-10-07 | 宁德市南海水产科技有限公司 | A kind of grouper scale artificial seedling rearing method |
| CN104285866A (en) * | 2014-10-23 | 2015-01-21 | 福建省水产研究所 | Technique for masculinizing large groupers by surgical implanting |
| CN109804951B (en) * | 2019-03-26 | 2022-02-18 | 海南晨海水产有限公司 | Artificial crossbreeding method for large-body mackerel and camel-back bass |
| CN109924149B (en) * | 2019-04-22 | 2021-05-28 | 宁德师范学院 | Method for promoting conversion of epinephelus akaara parents to males |
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| CN1444848A (en) * | 2003-01-27 | 2003-10-01 | 广东省大亚湾水产试验中心 | Industrial breeding method for bevel-band rockfish |
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| CN1444848A (en) * | 2003-01-27 | 2003-10-01 | 广东省大亚湾水产试验中心 | Industrial breeding method for bevel-band rockfish |
Non-Patent Citations (3)
| Title |
|---|
| 17α-甲基睾酮对赤点石斑鱼性逆转的影响. 方永强，林秋明，齐襄，洪桂英.水产学报，第16卷第2期. 1992 * |
| 点带石斑鱼的亲鱼培育、产卵受精和胚胎发育. 邹记兴，常林，向文洲，胡超群，林坚士.水生生物学报，第27卷第4期. 2003 * |
| 鞍带石斑鱼 Epinephelus lanceolatus (Bloch)繁殖生物学的初步研究. 杨洪志，梁荣峰.现代渔业信息，第17卷第7期. 2002 * |
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