CN103960184B - A kind of loach industrial artificial breeding improves the seedling-cultivating method of survival rate - Google Patents
A kind of loach industrial artificial breeding improves the seedling-cultivating method of survival rate Download PDFInfo
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Abstract
The invention belongs to technical field of aquaculture, particularly relate to the rationally loach industrial artificial breeding that is simple, strong operability of a kind of method and improve the seedling-cultivating method of survival rate.The method the time of fertilization can adopt natural mating method, squeeze smart method, can also be cut open the belly to get seminal vesicle method, greatly can reduce the lethality of breeding parent population, improves spawning rate, fertilization rate, incubation rate.<b />
Description
Technical field
The invention belongs to technical field of aquaculture, particularly relate to the seedling-cultivating method that the breeding of a kind of loach industrial artificial improves survival rate.
Background technology
Loach fat-containing component is lower, and cholesterol is less, and high protein and low fat food, containing a kind of unsaturated fatty acid of similar twenty carbon penetenoic acid, the anti-angiogenic aging of favourable human body, is of value to the elderly and cardiovascular patient.Have tonifying middle-Jiao and Qi, dehumidifying removing jaundice, kidney-nourishing is supporing yang, eliminating dampness to stop diarrhea, warming spleen and stomach, treatment hemorrhoid, only abnormal sweating effect.Be called as " ginseng in water ".
Loach breeding scale develops rapidly in recent years, and Miao ethnic group becomes the bottleneck of restriction industry development.At present, the wild[l that the seed of China's loach breeding is mainly manually fished for, due to pollutions such as chemical fertilizer, agricultural chemicals, three industrial wastes, cause loach wild resource to fall sharply, wild seed resource can not meet the needs of large-scale cultivation.How to carry out biological control, improve fertilization rate and the incubation rate of loach ovum.Seed rearing survival rate, reduces labour intensity and reproductive-cost is its key point.
As a kind of loach fish fry artificial propagation method that Chinese Patent Application No. is " 201310074705.3 ", this method all reduces the lethality of breeding parent population to a certain extent, improves spawning rate, fertilization rate, incubation rate.But the hatching pail that the method is used is the cylinder of diameter 15cm, high 40cm, this kind of hatching pail water consumption is large, and there is hatching dead angle, incubation rate is not high simultaneously.And unsticking operation was not carried out to it before fertilized egg, easily cause fertilized egg adhesion, reduce the incubation rate of fertilized egg.
Summary of the invention
The present invention is directed to the deficiency of existing loach seedling propagation technique, a kind of method rationally simple, strong operability is provided, the loach industrial artificial breeding of large-scale cultivation can improve the seedling-cultivating method of survival rate.The method can reduce the lethality of breeding parent population greatly, improves spawning rate, fertilization rate, incubation rate.
Above-mentioned technical problem of the present invention is implemented by the following technical programs:
The breeding of loach industrial artificial improves a seedling-cultivating method for survival rate, it is characterized in that, comprises the following steps:
A. parent selects
Select physical health, color and luster is normal, movable strong parent, the feeding water earthworm of 3 ~ 5 days;
B. artificial induced spawning
At water temperature 24 ~ 28 DEG C, carry out artificial induced spawning to female parent, injectable drug is luteinizing hormone releasing hormone A
2, Pan Li ketone, physiological saline, adopt intramuscular injection;
C. artificial insemination
Natural mating method: the female male parent of having hastened parturition is put in 2 ﹕ 1 ratios net cage of laying eggs, then puts into the cement pit that five faces are all smooth, treat its natural spawning fertilization;
D. incubating oosperm
After fertilization, by fertilized egg unsticking, viscidity-removing method is: in fertilized egg, add talcum powder in the ratio of Hua Shi Fen ﹕ fertilized egg=1 ﹕ 3, and then after the water adding 1 ~ 2 times stirs, use 80 mesh screen, after washing away talcum powder, fertilized egg is put into taper hatching pail and hatches, each taper hatching pail can hatch 5,000,000 ~ 7,000,000 loach seedlings, and hatching water temperature requires at 23 ~ 28 DEG C;
E. loach seedling is raised
After 48 hours, spray scatters in pond, just can feed loach seedling open-mouthed bait when starting travelling food finding, feeding quantity calculates by 0.5% of loach seedling gross weight, and feeding method is: be placed on by open-mouthed bait in plate, the ratio of putting a dish in every square metre is put, take out plate after two hours, from feeding first day, will carry out every day changing water, changing water method is: first bleed off the Chi Shui of in cement pit 1/3rd, and then injected clear water; When loach seedling grows to 2cm, cut off open-mouthed bait, make live body silk earthworm feeding into, still will carry out every day changing water, cement pit seedling density will control at 3000 ~ 5000 tails/m
2;
F. loach seedling is ripe
When after 12 days, loach seedling grows up to 3cm, just loach seedling can be put in a suitable place to breed in the large pool, also can start to sell as seed; Large pool cultivation 15 ~ 20 days, loach seedling can grow up to 6 ~ 8cm, until become commodity loach, also can cultivate commodity loach and sell in cement pit.
As preferably, injecting method concrete in described step B is: on operating desk, pad the cotton felt that 0.5 ~ 1cm is thick, separately do the circle of a 15cm diameter, upper seam gauze, in order to cover the loach parent be placed on cotton felt, adopt the mode of intramuscular injection by oxytocin injections in loach body.
As preferably, the processing method of described loach seedling open-mouthed bait is: 60% earthworm is worn into slurry and 10% and newly squeeze fresh milk and mix, add the white young material of 30% eel stir with agglomerating then make little granular.
As preferably, the mesh of the net cage of laying eggs in described step B is 9 orders, the thick 1mm of nettle, the large 2mm in hole, and specification is long 2m × wide 1m × high 0.5m.Net cage of laying eggs can prevent loach parent from eating ovum.
As preferably, the back cut diameter of described taper hatching pail is 1 meter, and lower port diameter 0.2 meter is high 1 meter.
As preferably, the water in described step C is pH=6.5 ~ 7 of underground water, water, and the dissolved oxygen amount of water is at least 8mg/L.
As preferably, described five the smooth cement pit degree of depth are 70 ~ 90cm.
As preferably, described fertilization method can also be crowded smart method: the seminal fluid manually extruding a male loach, then the normal saline dilution of 0.2% ~ 0.3% is added, the consumption of the physiological saline added is 1/3rd of the volume of the ovum that 3 female loach produce, then seminal fluid is poured in the ovum of 3 female loach products, and stir with goose feather, make it evenly be fertilized.Adopt the feritilization of ovum that the sperm extruded when squeezing smart method can be produced with 3 female loach.
As preferably, described fertilization method can also for taking seminal vesicle method: get a public loach, cut belly open, take out seminal vesicle and extrude seminal fluid, then add the normal saline dilution of 0.2% ~ 0.3%, the consumption of the physiological saline added is 1/3rd of the volume of the ovum that 10 female loach produce, is then poured into by seminal fluid in the ovum of 10 female loach products, and stir with goose feather, make it evenly be fertilized.Adopt the feritilization of ovum that the sperm extruded when taking seminal vesicle method can be produced with 10 female loach.
The present invention compared with prior art, has following advantage.
1. the method for artificial insemination that what this method mainly adopted is, namely can be natural mating method, squeeze smart method, can also be cut open the belly to get seminal vesicle method, adopt natural mating method, male parent can be survived and be continued to raise, protection parent that can be best is injury-free, adopt and squeeze smart method, male parent also can also continue to survive several days, in in the past few days, male parent is sold as the loach that lives, also higher economic worth can be obtained, and adopt and cut open the belly and get seminal vesicle method, although the sperm of maximum can be obtained, but male parent will be dead, loach of seeking death can only be worked as sell, economic worth is lower.
2. when this method is to female male parent oxytocin injection, made specific purpose tool by oneself, prevent direct hand from grabbing the female male parent of damage, improve the spawning rate of female male parent, and the lethality of parent also can within 2%, parent's lethality reduces 13%.
3. the present invention is to the sperm the taken out normal saline dilution of 0.2% ~ 0.3%, its object is to activate sperm, increases sperm viability.
4. the water used in this method is underground water, pH=6.5 ~ 7 of water, and the dissolved oxygen amount of water is at least 8mg/L.The content of microorganisms of underground water is low, and the content of hydrogen sulphide, nitrogen oxide is also lower, is more conducive to the growth of seedling.
5. cement pit five face in the present invention is all smooth, is beneficial to clean, removes the pest in pond, can better control ph.
6. the benefit adopting taper hatching pail to have has: using water wisely, than the water consumption of conventional incubation case hatching saving 2/3rds; Increase the dissolved oxygen amount of water, make the dissolved oxygen amount of water all the time higher than 8mg/L; Add hatching density, a taper hatching pail is better than tens 20m
2the hatching amount in pond, a traditional 20m
2hatchery can put two teams parent and hatch about 1,000,000 loach seedlings, and a hatching pail just can hatch 500 ~ 7,000,000 loach seedlings, and the occupation of land of a hatching pail only has 1m
2, therefore saved land used greatly, improve the incubation rate of unit are; Taper hatching pail is compared and columniform hatching pail, decreases dead angle, increases to float to incubate momentum, improves incubation rate.
Be more than 98% by parent's survival rate after this mating system, improve 13%, spawning rate is more than 98%, improves 7%, and fertilization rate more than 98.5%, improves 1%, and incubation rate more than 99.2%, improves 7.8%, and seedling percent is more than 85%, improves 65%.
Embodiment
Below by embodiment, technical scheme of the present invention is described in further detail:
Embodiment 1: a kind of loach industrial artificial breeding improves the seedling-cultivating method of survival rate, comprises the following steps:
A. parent selects
Select physical health, color and luster is normal, movable strong parent, the feeding water earthworm of 3 ~ 5 days;
B. artificial induced spawning
At water temperature 24 ~ 28 DEG C, carry out artificial induced spawning to female parent, injectable drug is luteinizing hormone releasing hormone A
2125mg, Pan Li ketone (DOM) 100mg, physiological saline 80cc, three's hybrid injection, adopts intramuscular injection, must not by infusion of medicine abdominal cavity, and every endnote penetrates 0.5cc; During injection, the cotton felt that on operating desk pads, 0.5 ~ 1cm is thick, separately does the circle of a 15cm diameter, and upper seam gauze, in order to cover the loach parent be placed on cotton felt, is kept away manual-free and grabbed, damage loach parent;
C. artificial insemination
Natural mating method: the female male parent of having hastened parturition is put in 2 ﹕ 1 ratios net cage of laying eggs, then the cement pit that five faces are all smooth is put into, treat its natural spawning fertilization, the degree of depth of cement pit is 70 ~ 90cm, the mesh of net cage of laying eggs is 9 orders, the thick 1mm of nettle, the large 2mm in hole, specification is long 2m × wide 1m × high 0.5m;
D. incubating oosperm
After fertilization, by fertilized egg unsticking, viscidity-removing method is: in fertilized egg, add talcum powder in the ratio of Hua Shi Fen ﹕ fertilized egg=1 ﹕ 3, and then after the water adding 1 ~ 2 times stirs, uses 80 mesh screen, after washing away talcum powder, it is 1 meter that fertilized egg is put into back cut diameter, lower port diameter 0.2 meter, hatching in the taper hatching pail of high 1 meter, each taper hatching pail can hatch 5,000,000 ~ 7,000,000 loach seedlings, and hatching water temperature requires at 23 ~ 28 DEG C;
E. loach seedling is raised
After 48 hours, spray scatters in pond, just can feed loach seedling open-mouthed bait when starting travelling food finding, feeding quantity calculates by 0.5% of loach seedling gross weight, and feeding method is: be placed on by open-mouthed bait in plate, the ratio of putting a dish in every square metre is put, take out plate after two hours, from feeding first day, will carry out every day changing water, changing water method is: first bleed off the Chi Shui of in cement pit 1/3rd, and then injected clear water; When loach seedling grows to 2cm, cut off open-mouthed bait, make live body silk earthworm feeding into, will carry out every day changing water, cement pit seedling density will control at 3000 ~ 5000 tails/m
2;
F. loach seedling is ripe
When after 12 days, loach seedling grows up to 3cm, just loach seedling can be put in a suitable place to breed in the large pool, also can start to sell as seed; Large pool cultivation 15 ~ 20 days, loach seedling can grow up to 6 ~ 8cm, until become commodity loach, also can cultivate commodity loach and sell in cement pit.
The processing method of described loach seedling open-mouthed bait is: 60% earthworm is worn into slurry and 10% and newly squeeze fresh milk and mix, add the white young material of 30% eel stir with agglomerating then make little granular.
Water in cement pit is pH=6.5 ~ 7 of underground water, water, and the dissolved oxygen amount of water is at least 8mg/L.
Breed loach seedling by the method, greatly can reduce the lethality of parent, improve the survival rate of seedling.Parent's survival rate is 100%, and spawning rate is 98%, fertilization rate 98.5%, incubation rate 99.3%, and seedling percent is 86%.
The method can realize loach seedling factorial praluction, for loach breeding provides a large amount of seeds, simultaneously can maximized protection parent not dead, there is good economic benefit.
Embodiment 2: a kind of loach industrial artificial breeding improves the seedling-cultivating method of survival rate, comprises the following steps:
A. parent selects
Select physical health, color and luster is normal, movable strong parent, the feeding water earthworm of 3 ~ 5 days;
B. artificial induced spawning
At water temperature 24 ~ 28 DEG C, carry out artificial induced spawning to female parent, injectable drug is luteinizing hormone releasing hormone A
2125mg, Pan Li ketone (DOM) 100mg, physiological saline 80cc, three's hybrid injection, adopts intramuscular injection, must not by infusion of medicine abdominal cavity, and every endnote penetrates 0.5cc; During injection, the cotton felt that on operating desk pads, 0.5 ~ 1cm is thick, separately does the circle of a 15cm diameter, and upper seam gauze, in order to cover the loach parent be placed on cotton felt, is kept away manual-free and grabbed, damage loach parent;
C. artificial insemination
Squeeze smart method: the seminal fluid manually extruding a male loach, then add the normal saline dilution of 0.2% ~ 0.3%, the consumption of the physiological saline added is 1/3rd of the volume of the ovum that 3 female loach produce, is then poured into by seminal fluid in the ovum of 3 female loach products, and stir with goose feather, make it evenly be fertilized;
D. incubating oosperm
After fertilization, by fertilized egg unsticking, viscidity-removing method is: in fertilized egg, add talcum powder in the ratio of Hua Shi Fen ﹕ fertilized egg=1 ﹕ 3, and then after the water adding 1 ~ 2 times stirs, uses 80 mesh screen, after washing away talcum powder, it is 1 meter that fertilized egg is put into back cut diameter, lower port diameter 0.2 meter, hatching in the taper hatching pail of high 1 meter, each taper hatching pail can hatch 5,000,000 ~ 7,000,000 loach seedlings, and hatching water temperature requires at 23 ~ 28 DEG C;
E. loach seedling is raised
After 48 hours, spray scatters in pond, just can feed loach seedling open-mouthed bait when starting travelling food finding, feeding quantity calculates by 0.5% of loach seedling gross weight, and feeding method is: be placed on by open-mouthed bait in plate, the ratio of putting a dish in every square metre is put, take out plate after two hours, from feeding first day, will carry out every day changing water, changing water method is: first bleed off the Chi Shui of in cement pit 1/3rd, and then injected clear water; When loach seedling grows to 2cm, cut off open-mouthed bait, make live body silk earthworm feeding into, will carry out every day changing water, cement pit seedling density will control at 3000 ~ 5000 tails/m
2;
F. loach seedling is ripe
When after 12 days, loach seedling grows up to 3cm, just loach seedling can be put in a suitable place to breed in the large pool, also can start to sell as seed; Large pool cultivation 15 ~ 20 days, loach seedling can grow up to 6 ~ 8cm, until become commodity loach, also can cultivate commodity loach and sell in cement pit.
The processing method of described loach seedling open-mouthed bait is: 60% earthworm is worn into slurry and 10% and newly squeeze fresh milk and mix, add the white young material of 30% eel stir with agglomerating then make little granular.
Water in cement pit is pH=6.5 ~ 7 of underground water, water, and the dissolved oxygen amount of water is at least 8mg/L.
Adopted by the method the time of fertilization and squeeze smart method, while can obtaining relatively large sperm, parent can be made again to survive, be beneficial to sale, ensure that the economic worth of parent, parent's survival rate is 100%.See on the whole, by this propagation method, spawning rate is 98.4%, fertilization rate 98.8%, incubation rate 99.5%, and seedling percent is 89%.
The method can realize loach seedling factorial praluction, for loach breeding provides a large amount of seeds, has good economic benefit.
Embodiment 3: a kind of loach industrial artificial breeding improves the seedling-cultivating method of survival rate, comprises the following steps:
A. parent selects
Select physical health, color and luster is normal, movable strong parent, the feeding water earthworm of 3 ~ 5 days;
B. artificial induced spawning
At water temperature 24 ~ 28 DEG C, carry out artificial induced spawning to female parent, injectable drug is luteinizing hormone releasing hormone A
2125mg, Pan Li ketone (DOM) 100mg, physiological saline 80cc, three's hybrid injection, adopts intramuscular injection, must not by infusion of medicine abdominal cavity, and every endnote penetrates 0.5cc; During injection, the cotton felt that on operating desk pads, 0.5 ~ 1cm is thick, separately does the circle of a 15cm diameter, and upper seam gauze, in order to cover the loach parent be placed on cotton felt, is kept away manual-free and grabbed, damage loach parent;
C. artificial insemination
Take seminal vesicle method: get a public loach, cut belly open, take out seminal vesicle and extrude seminal fluid, then the normal saline dilution of 0.2% ~ 0.3% is added, the consumption of the physiological saline added is 1/3rd of the volume of the ovum that 10 female loach produce, then seminal fluid is poured in the ovum of 10 female loach products, and stir with goose feather, make it evenly be fertilized;
D. incubating oosperm
After fertilization, by fertilized egg unsticking, viscidity-removing method is: in fertilized egg, add talcum powder in the ratio of Hua Shi Fen ﹕ fertilized egg=1 ﹕ 3, and then after the water adding 1 ~ 2 times stirs, uses 80 mesh screen, after washing away talcum powder, it is 1 meter that fertilized egg is put into back cut diameter, lower port diameter 0.2 meter, hatching in the taper hatching pail of high 1 meter, each taper hatching pail can hatch 5,000,000 ~ 7,000,000 loach seedlings, and hatching water temperature requires at 23 ~ 28 DEG C;
E. loach seedling is raised
After 48 hours, spray scatters in pond, just can feed loach seedling open-mouthed bait when starting travelling food finding, feeding quantity calculates by 0.5% of loach seedling gross weight, and feeding method is: be placed on by open-mouthed bait in plate, the ratio of putting a dish in every square metre is put, take out plate after two hours, from feeding first day, will carry out every day changing water, changing water method is: first bleed off the Chi Shui of in cement pit 1/3rd, and then injected clear water; When loach seedling grows to 2cm, cut off open-mouthed bait, make live body silk earthworm feeding into, will carry out every day changing water, cement pit seedling density will control at 3000 ~ 5000 tails/m
2;
F. loach seedling is ripe
When after 12 days, loach seedling grows up to 3cm, just loach seedling can be put in a suitable place to breed in the large pool, also can start to sell as seed; Large pool cultivation 15 ~ 20 days, loach seedling can grow up to 6 ~ 8cm, until become commodity loach, also can cultivate commodity loach and sell in cement pit.
The processing method of described loach seedling open-mouthed bait is: 60% earthworm is worn into slurry and 10% and newly squeeze fresh milk and mix, add the white young material of 30% eel stir with agglomerating then make little granular.
Water in cement pit is pH=6.5 ~ 7 of underground water, water, and the dissolved oxygen amount of water is at least 8mg/L.
Breed loach seedling by the method, the acquisition sperm quantity of maximizing, but male parent is by death, can only processes as early as possible and sell.Spawning rate is 100%, fertilization rate 99.5%, incubation rate 99.5%, and seedling percent is 90%.
The method can realize loach seedling factorial praluction, for loach breeding provides a large amount of seeds, has good economic benefit.
Specific embodiment described herein is only to the explanation for example of the present invention's spirit.Those skilled in the art can make various amendment or supplement or adopt similar mode to substitute to described specific embodiment, but can't depart from spirit of the present invention or surmount the scope that appended claims defines.
Claims (9)
1. the breeding of loach industrial artificial improves a seedling-cultivating method for survival rate, it is characterized in that, comprises the following steps:
A. parent selects
Select physical health, color and luster is normal, movable strong parent, the feeding water earthworm of 3 ~ 5 days;
B. artificial induced spawning
At water temperature 24 ~ 28 DEG C, carry out artificial induced spawning to female male parent, injectable drug is luteinizing hormone releasing hormone A
2, Pan Li ketone, physiological saline, adopt intramuscular injection;
C. be fertilized
Natural mating method: the female male parent of having hastened parturition is put in 2 ﹕ 1 ratios net cage of laying eggs, then puts into the cement pit that five faces are all smooth, treat its natural spawning fertilization;
D. incubating oosperm
After fertilization, by fertilized egg unsticking, viscidity-removing method is: in fertilized egg, add talcum powder in the ratio of Hua Shi Fen ﹕ fertilized egg=1 ﹕ 3, and then after the water adding 1 ~ 2 times stirs, use 80 mesh screen, after washing away talcum powder, fertilized egg is put into taper hatching pail and hatches, each taper hatching pail can hatch 5,000,000 ~ 7,000,000 loach seedlings, and hatching water temperature requires at 23 ~ 28 DEG C;
E. loach seedling is raised
After 48 hours, spray scatters in pond, just can feed loach seedling open-mouthed bait when starting travelling food finding, feeding quantity calculates by 0.5% of loach seedling gross weight, and feeding method is: be placed on by open-mouthed bait in plate, the ratio of putting a dish in every square metre is put, take out plate after two hours, from feeding first day, will carry out every day changing water, changing water method is: first bleed off the Chi Shui of in cement pit 1/3rd, and then injected clear water; When loach seedling grows to 2cm, cut off open-mouthed bait, make live body silk earthworm feeding into, still will carry out every day changing water, cement pit seedling density will control at 3000 ~ 5000 tails/m
2;
F. loach seedling is ripe
When after 12 days, loach seedling grows up to 3cm, just loach seedling can be put in a suitable place to breed in the large pool, or starts to sell as seed; Large pool cultivation 15 ~ 20 days, loach seedling can grow up to 6 ~ 8cm, sell, or cultivation was sold to commodity loach in cement pit until become commodity loach.
2. loach industrial artificial breeding according to claim 1 improves the seedling-cultivating method of survival rate, it is characterized in that, injecting method concrete in described step B is: on operating desk, pad the cotton felt that 0.5 ~ 1cm is thick, separately do the circle of a 15cm diameter, upper seam gauze, in order to cover the loach parent be placed on cotton felt, adopt the mode of intramuscular injection by oxytocin injections in loach body.
3. loach industrial artificial breeding according to claim 1 improves the seedling-cultivating method of survival rate, it is characterized in that, the processing method of described loach seedling open-mouthed bait is: 60% earthworm is worn into slurry and 10% and newly squeeze fresh milk and mix, add the white young material of 30% eel stir with agglomerating then make little granular.
4. loach industrial artificial breeding according to claim 1 improves the seedling-cultivating method of survival rate, and it is characterized in that, the mesh of described net cage of laying eggs is 9 orders, the thick 1mm of nettle, the large 2mm in hole, and specification is long 2m × wide 1m × high 0.5m.
5. loach industrial artificial breeding according to claim 1 improves the seedling-cultivating method of survival rate, and it is characterized in that, the back cut diameter of described taper hatching pail is 1 meter, and lower port diameter 0.2 meter is high 1 meter.
6. loach industrial artificial according to claim 1 breeding improves the seedling-cultivating method of survival rate, and it is characterized in that, the water in described step e is pH=6.5 ~ 7 of underground water, water, and the dissolved oxygen amount of water is at least 8mg/L.
7. loach industrial artificial according to claim 1 breeding improves the seedling-cultivating method of survival rate, it is characterized in that, described five the smooth cement pit degree of depth are 70 ~ 90cm.
8. loach industrial artificial breeding according to claim 1 improves the seedling-cultivating method of survival rate, it is characterized in that, fertilization method is for squeezing smart method: the seminal fluid manually extruding a male loach, then the normal saline dilution of 0.2% ~ 0.3% is added, the consumption of the physiological saline added is 1/3rd of the volume of the ovum that 3 female loach produce, then seminal fluid is poured in the ovum of 3 female loach products, and stir with goose feather, make it evenly be fertilized.
9. loach industrial artificial breeding according to claim 1 improves the seedling-cultivating method of survival rate, it is characterized in that, fertilization method is for taking seminal vesicle method: get a public loach, cut belly open, take out seminal vesicle and extrude seminal fluid, then add the normal saline dilution of 0.2% ~ 0.3%, the consumption of the physiological saline added is 1/3rd of the volume of the ovum that 10 female loach produce, then seminal fluid is poured in the ovum of 10 female loach products, and stir with goose feather, make it evenly be fertilized.
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CN109699530B (en) * | 2019-01-25 | 2021-09-07 | 佛山市嘉沃农业科技合伙企业(有限合伙) | Artificial spawning induction method for loach culture |
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