CN104285866A - Technique for masculinizing large groupers by surgical implanting - Google Patents
Technique for masculinizing large groupers by surgical implanting Download PDFInfo
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- CN104285866A CN104285866A CN201410567078.1A CN201410567078A CN104285866A CN 104285866 A CN104285866 A CN 104285866A CN 201410567078 A CN201410567078 A CN 201410567078A CN 104285866 A CN104285866 A CN 104285866A
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- grouper
- hormone
- medicinal strip
- groupers
- fish
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- 241001417495 Serranidae Species 0.000 title claims abstract description 48
- 238000000034 method Methods 0.000 title abstract description 5
- 230000001356 masculinizing effect Effects 0.000 title abstract 2
- 241000251468 Actinopterygii Species 0.000 claims abstract description 26
- 229940088597 hormone Drugs 0.000 claims abstract description 17
- 239000005556 hormone Substances 0.000 claims abstract description 17
- 210000003205 muscle Anatomy 0.000 claims abstract description 14
- 239000007943 implant Substances 0.000 claims abstract description 8
- 108010010803 Gelatin Proteins 0.000 claims description 12
- 239000003814 drug Substances 0.000 claims description 12
- 229920000159 gelatin Polymers 0.000 claims description 12
- 239000008273 gelatin Substances 0.000 claims description 12
- 235000019322 gelatine Nutrition 0.000 claims description 12
- 235000011852 gelatine desserts Nutrition 0.000 claims description 12
- 230000037396 body weight Effects 0.000 claims description 10
- 229920000084 Gum arabic Polymers 0.000 claims description 9
- 241000978776 Senegalia senegal Species 0.000 claims description 9
- 235000010489 acacia gum Nutrition 0.000 claims description 9
- 239000000205 acacia gum Substances 0.000 claims description 9
- 238000005516 engineering process Methods 0.000 claims description 9
- RRAFCDWBNXTKKO-UHFFFAOYSA-N eugenol Chemical compound COC1=CC(CC=C)=CC=C1O RRAFCDWBNXTKKO-UHFFFAOYSA-N 0.000 claims description 6
- 239000011521 glass Substances 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 6
- 239000002245 particle Substances 0.000 claims description 6
- 239000000243 solution Substances 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 230000013011 mating Effects 0.000 claims description 4
- NPBVQXIMTZKSBA-UHFFFAOYSA-N Chavibetol Natural products COC1=CC=C(CC=C)C=C1O NPBVQXIMTZKSBA-UHFFFAOYSA-N 0.000 claims description 3
- 239000005770 Eugenol Substances 0.000 claims description 3
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 claims description 3
- 229930182566 Gentamicin Natural products 0.000 claims description 3
- UVMRYBDEERADNV-UHFFFAOYSA-N Pseudoeugenol Natural products COC1=CC(C(C)=C)=CC=C1O UVMRYBDEERADNV-UHFFFAOYSA-N 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 239000003153 chemical reaction reagent Substances 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 229960002217 eugenol Drugs 0.000 claims description 3
- 229960002518 gentamicin Drugs 0.000 claims description 3
- 238000007496 glass forming Methods 0.000 claims description 3
- 238000002513 implantation Methods 0.000 claims description 3
- 238000002347 injection Methods 0.000 claims description 3
- 239000007924 injection Substances 0.000 claims description 3
- 238000003780 insertion Methods 0.000 claims description 3
- 230000037431 insertion Effects 0.000 claims description 3
- 235000011837 pasties Nutrition 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 238000007711 solidification Methods 0.000 claims description 3
- 230000008023 solidification Effects 0.000 claims description 3
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 239000010935 stainless steel Substances 0.000 claims description 3
- 235000015112 vegetable and seed oil Nutrition 0.000 claims description 3
- 239000008158 vegetable oil Substances 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- GCKMFJBGXUYNAG-UHFFFAOYSA-N 17alpha-methyltestosterone Natural products C1CC2=CC(=O)CCC2(C)C2C1C1CCC(C)(O)C1(C)CC2 GCKMFJBGXUYNAG-UHFFFAOYSA-N 0.000 abstract description 3
- GCKMFJBGXUYNAG-HLXURNFRSA-N Methyltestosterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@](C)(O)[C@@]1(C)CC2 GCKMFJBGXUYNAG-HLXURNFRSA-N 0.000 abstract description 3
- 206010049290 Feminisation acquired Diseases 0.000 abstract 1
- 208000034793 Feminization Diseases 0.000 abstract 1
- 208000027418 Wounds and injury Diseases 0.000 abstract 1
- 238000011109 contamination Methods 0.000 abstract 1
- 230000006378 damage Effects 0.000 abstract 1
- 208000014674 injury Diseases 0.000 abstract 1
- 238000001356 surgical procedure Methods 0.000 abstract 1
- 241000357444 Epinephelus coioides Species 0.000 description 2
- 241000246500 Gentianella aspera Species 0.000 description 2
- 210000000582 semen Anatomy 0.000 description 2
- 208000027877 Disorders of Sex Development Diseases 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241001596774 Epinephelus akaara Species 0.000 description 1
- 206010015150 Erythema Diseases 0.000 description 1
- 206010067268 Post procedural infection Diseases 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 231100000321 erythema Toxicity 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 201000005611 hermaphroditism Diseases 0.000 description 1
- 230000010196 hermaphroditism Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 231100000225 lethality Toxicity 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000007758 mating behavior Effects 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000019100 sperm motility Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 208000013327 true hermaphroditism Diseases 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Veterinary Medicine (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Biodiversity & Conservation Biology (AREA)
- Reproductive Health (AREA)
- Animal Husbandry (AREA)
- Marine Sciences & Fisheries (AREA)
- Wood Science & Technology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Medicinal Preparation (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention relates to a technique for masculinizing large groupers by surgical implanting. According to the technique, individual large groupers more than four years old are selected, manually prepared hormone sticks containing 17Alpha-methyltestosterone are implanted into dorsal muscles of the selected groupers in a surgical manner 6 months before a natural propagation season so as to masculinize the individual large groupers, the individual large groupers mature on the propagation season and become male parent fishes required by artificial grouper propagation. The technique has the advantages that by surgically implanting the hormone sticks, the large groupers can be masculinized at a higher rate up to 100%, with no re-feminization occurring; dosage of the surgically implanted hormone sticks is easy to control, implants act for a lifetime after being implanted, no enhanced supplementary implanting is required, the surgery is simple and reliable, and little injury is caused; the hormone sticks are simple to make, controlling is easy, no contamination occurs, and the hormone sticks made can be stored for a long period of time.
Description
Technical field
The present invention relates to a kind of large-scale grouper Operation formula masculine technology.
Background technology
Grouper is the kind that in seawater fish, economic worth is the highest.In more than 100 kind, whole world grouper, by its adult specification, large-scale and small-sized two classes can be divided into.Small-sized grouper is exactly the grouper of the such small individual of our common epinephelus akaara (erythema) and green hata (native spot), and its surface is, individual little, known maximum individuality is generally at below 1-2kg; Growth is slow, propagates two anniversary maximum whose body weights artificially and be generally no more than 1kg from fingerling.The kind of large-scale grouper is a lot, and common economic species has Epinephelus coioides, saladifish, brown grouper, rough gentian grouper etc., and individuality is all comparatively large, and known maximum individuality generally can reach tens even hundreds of kg; Growth is fast, cultivates general 1 year and can reach 1kg even larger from fingerling.Due to the success of grouper artificial propagation and large-scale artificial nursery, its cultivation increases very fast in recent years.This growth mainly relies on the growth of large-scale Grouper cultivating amount.But the grouper protogynous kind that is hermaphroditism, small-sized grouper is easy to realize masculine and can occurs the male of a greater number in colony, and large-scale grouper male not easily obtains.All large-scale grouper kinds, at least needed just may occur male 7 ages vegetative period; In a locality microcommunity, a male can only be had to exist.Lacking male parent population, is the main restricting factor of large-scale grouper workman breeding.
Summary of the invention
Object of the present invention to provide a kind of large-scale grouper Operation formula masculine technology, adopts the mode of Operation hormone medicinal strip, make female individuals realize masculine, and producing for the artificial propagation of large-scale grouper and nursery provides male parent population.
The present invention realizes like this, described large-scale grouper Operation formula masculine technology, it is characterized in that: select large-scale grouper individuality in four ages more than, in first 6 months of season of natural propagation, with the hormone medicinal strip manually prepared, implant in grouper dorsal muscles with modus operandi, make grouper individuality realize masculine, reach maturity when mating season arrives, become the male parent population needed for grouper artificial propagation.
The way of the hormone medicinal strip of artificial preparation of the present invention is, get two 100ml beakers, 18ml distilled water is added respectively with pipette, a beaker adds 2g gelatin particle, another beaker adds 2g gum Arabic particle, make it dissolve with alcolhol burner heating respectively, stir gently with glass bar during heating and make it be heated to be uniformly dissolved, to make the gelatin solution of 10% and the gumwater of 10%; Get a 50ml beaker again, add 10g hormone pulvis, i.e. 17 α-Methyltestosterone, 17 Alpha-Methyl stosterones, purity >=97%, and a vegetable oil, when gelatin and gum Arabic are cooled to close to normal temperature, get 2.5ml 10% aqueous gelatin solution and the 2.5ml 10% gum Arabic aqueous solution with pipette to add and place in the beaker of hormone, stir with glass bar and make it evenly become pasty state, then medicine group being moved into volume is in the glass forming disk of long 2cm, wide 2cm, thick 0.2cm; Medicament is paved, makes it in forming disk, become the tabular of long 2cm, wide 2cm, thick 0.2cm; After one hour, remove solidification web frame, with knife blade, tabular medicament not yet completely crued in forming disk is pressed interval crosscut 9 cutter of 0.2cm, made medicament long, the cross sections of 10 2cm be the medicinal strip of 0.2cm × 0.2cm, can load after medicinal strip solidifies completely that airtight in 50ml reagent bottle to put into refrigerator stand-by.
The way that modus operandi of the present invention is implanted in grouper dorsal muscles is, selecting needs the large-scale grouper of the health of masculine individual, moves in the small-sized net cage of pool side convenient operation, fish is first anaesthetized with eugenol by operation consent, with fish folder, fish is clamped, be placed on operating table, expose fish dorsal; On dorsal muscles, cut the edge of a knife of a 0.5cm with knife blade, then use poker: long 10cm, diameter 0.3cm, stainless steel, one end is sharp-pointed, and one holds blunt needle end level with both hands, become 30 ° ~ 45 ° angles to insert dorsal muscles from the edge of a knife with the fish body longitudinal axis, the degree of depth of insertion is generally 5cm; Take out poker, insert dorsal muscles with the passage that tweezers folder medicinal strip inserts from knife-edge part along poker, with the flat blunt end of poker, medicinal strip is headed into passage deep; Medicinal strip consumption is relevant to fish body weight, and fish body body weight is less than 5kg only needs implantation half root, the medicinal strip that namely 1cm is long; Fish body body weight 5 ~ 10kg need implant 1, i.e. the medicinal strip of long 2cm; The scalpel edge region of perioperatively fish body need be cleaned with gentamicin injection.
The invention has the beneficial effects as follows, large-scale grouper adopts Operation medicinal strip mode, and male rate is high, can reach 100%, and there will not be again female phenomenon; The pharmaceutical quantities of Operation medicinal strip mode is easy to control, and once implants throughout one's life effectively, implanting, performing the operation simple and reliable, damaging little without the need to strengthening to supplement; Medicinal strip preparation method is simple, is easy to control, and not contaminated, the medicinal strip made can long storage time.
Embodiment
Large-scale grouper Operation formula masculine technology of the present invention, the steps include:
1, the making of hormone medicinal strip: get two 100ml beakers, 18ml distilled water is added respectively with pipette, a beaker adds 2g gelatin particle, another beaker adds 2g gum Arabic particle, it is made to dissolve with alcolhol burner heating respectively, stir gently with glass bar during heating and make it be heated to be uniformly dissolved, to make the gelatin solution of 10% and the gumwater of 10%.Get a 50ml beaker again, add 10g hormone pulvis (17 α-Methyltestosterone, 17 Alpha-Methyl stosterones, purity >=97%, imported from America) and a vegetable oil, when gelatin and gum Arabic are cooled to close to normal temperature, get 2.5ml 10% aqueous gelatin solution and the 2.5ml 10% gum Arabic aqueous solution with pipette to add and place in the beaker of hormone, stirring with glass bar makes it evenly become pasty state, and then medicine group being moved into volume is that 2cm (length) × 2cm(is wide) × 0.2cm(is thick) glass forming disk in; Paved by medicament, 2cm (length) × 2cm(is wide to make it become in forming disk) × 0.2cm(is thick) tabular; After one hour, remove solidification web frame, with knife blade, tabular medicament not yet completely crued in forming disk press interval crosscut 9 cutter of 0.2cm, made medicament 10 2cm grow, cross sections are the medicinal strip of 0.2cm × 0.2cm.Can load after medicinal strip solidifies completely that airtight in 50ml reagent bottle to put into refrigerator stand-by.All manufacturing process need be carried out in aseptic operation box above.
2, implant fish body: in mating season first about 6 months, select to need the large-scale grouper adult of the health of masculine, move in the small-sized net cage of pool side convenient operation.Fish is first anaesthetized with eugenol by operation consent, is clamped by fish, be placed on operating table, expose fish dorsal with fish folder; On dorsal muscles, cut the edge of a knife of a 0.5cm with knife blade, then use poker (long 10cm, diameter 0.3cm, stainless steel, one end is sharp-pointed, one hold level with both hands blunt) needle end become 30 ° ~ 45 ° angles to insert dorsal muscles from the edge of a knife with the fish body longitudinal axis, the degree of depth of insertion is generally 5cm; Take out poker, insert dorsal muscles with the passage that tweezers folder medicinal strip inserts from knife-edge part along poker, with the passivity end of poker, medicinal strip is headed into passage deep.Medicinal strip consumption is relevant to fish body weight, and fish body body weight is less than 5kg only needs implantation half root (1cm is long) medicinal strip; Fish body body weight 5 ~ 10kg need implant 1 (long 2cm) medicinal strip.The scalpel edge region of perioperatively fish body need be cleaned with gentamicin injection.
The tens of example tests of the present invention on the large-scale groupers such as Epinephelus coioides, saladifish, brown grouper, rough gentian grouper, result shows: after medicinal strip is planted and buried, and the male rate of this season reaches 100%; Postoperative infection rate and lethality are 0.The observation of underwent operative 5-8, not yet finds multiple female phenomenon.After masculine there is mating look at reproductive stage body surface in sexually matured parent population, has mating behavior to occur; Extruding belly gonopore can discharge seminal fluid, and under sediments microscope inspection, sperm motility enlivens; The seminal fluid that milter produces of masculine when raun lays eggs can make ovum complete fertilization process, and normal development.
Claims (3)
1. large-scale grouper Operation formula masculine technology, it is characterized in that: select large-scale grouper individuality in four ages more than, in first 6 months of season of natural propagation, with the hormone medicinal strip manually prepared, implant in grouper dorsal muscles with modus operandi, make grouper individuality realize masculine, reach maturity when mating season arrives, become the male parent population needed for grouper artificial propagation.
2. large-scale grouper Operation formula masculine technology according to claim 1, it is characterized in that: the way of the hormone medicinal strip of described artificial preparation is, get two 100ml beakers, 18ml distilled water is added respectively with pipette, a beaker adds 2g gelatin particle, and another beaker adds 2g gum Arabic particle, makes it dissolve respectively with alcolhol burner heating, stir gently with glass bar during heating and make it be heated to be uniformly dissolved, to make the gelatin solution of 10% and the gumwater of 10%; Get a 50ml beaker again, add 10g hormone pulvis, i.e. 17 Alpha-Methyl stosterones, purity >=97%, with a vegetable oil, when gelatin and gum Arabic are cooled to close to normal temperature, get 2.5ml 10% aqueous gelatin solution and the 2.5ml 10% gum Arabic aqueous solution with pipette and add and place in the beaker of hormone, stirring with glass bar makes it evenly become pasty state, and then medicine group being moved into volume is in the glass forming disk of long 2cm, wide 2cm, thick 0.2cm; Medicament is paved, makes it in forming disk, become the tabular of long 2cm, wide 2cm, thick 0.2cm; After one hour, remove solidification web frame, with knife blade, tabular medicament not yet completely crued in forming disk is pressed interval crosscut 9 cutter of 0.2cm, made medicament long, the cross sections of 10 2cm be the medicinal strip of 0.2cm × 0.2cm, can load after medicinal strip solidifies completely that airtight in 50ml reagent bottle to put into refrigerator stand-by.
3. large-scale grouper Operation formula masculine technology according to claim 1, it is characterized in that: the way that described modus operandi is implanted in grouper dorsal muscles is, select to need the large-scale grouper of the health of masculine individual, move in the small-sized net cage of pool side convenient operation, fish is first anaesthetized with eugenol by operation consent, with fish folder, fish is clamped, be placed on operating table, expose fish dorsal; On dorsal muscles, cut the edge of a knife of a 0.5cm with knife blade, then use poker: long 10cm, diameter 0.3cm, stainless steel, one end is sharp-pointed, and one holds blunt needle end level with both hands, become 30 ° ~ 45 ° angles to insert dorsal muscles from the edge of a knife with the fish body longitudinal axis, the degree of depth of insertion is generally 5cm; Take out poker, insert dorsal muscles with the passage that tweezers folder medicinal strip inserts from knife-edge part along poker, with the flat blunt end of poker, medicinal strip is headed into passage deep; Medicinal strip consumption is relevant to fish body weight, and fish body body weight is less than 5kg only needs implantation half root, the medicinal strip that namely 1cm is long; Fish body body weight 5 ~ 10kg need implant 1, i.e. the medicinal strip of long 2cm; The scalpel edge region of perioperatively fish body need be cleaned with gentamicin injection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410567078.1A CN104285866A (en) | 2014-10-23 | 2014-10-23 | Technique for masculinizing large groupers by surgical implanting |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410567078.1A CN104285866A (en) | 2014-10-23 | 2014-10-23 | Technique for masculinizing large groupers by surgical implanting |
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| Publication Number | Publication Date |
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| CN104285866A true CN104285866A (en) | 2015-01-21 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410567078.1A Pending CN104285866A (en) | 2014-10-23 | 2014-10-23 | Technique for masculinizing large groupers by surgical implanting |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104808005A (en) * | 2015-04-15 | 2015-07-29 | 中山大学 | Method for identifying male giant grouper |
| CN105900864A (en) * | 2016-04-13 | 2016-08-31 | 集美大学 | Large grouper operation interpolative masculine method |
| CN107197792A (en) * | 2017-05-10 | 2017-09-26 | 中山大学 | A kind of method for controlling Population quickly to obtain grouper feature milter |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104808005A (en) * | 2015-04-15 | 2015-07-29 | 中山大学 | Method for identifying male giant grouper |
| CN105900864A (en) * | 2016-04-13 | 2016-08-31 | 集美大学 | Large grouper operation interpolative masculine method |
| CN107197792A (en) * | 2017-05-10 | 2017-09-26 | 中山大学 | A kind of method for controlling Population quickly to obtain grouper feature milter |
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