CN105766804A - Artificial breeding method of cotesia chilonis matsumura - Google Patents
Artificial breeding method of cotesia chilonis matsumura Download PDFInfo
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- CN105766804A CN105766804A CN201610155896.XA CN201610155896A CN105766804A CN 105766804 A CN105766804 A CN 105766804A CN 201610155896 A CN201610155896 A CN 201610155896A CN 105766804 A CN105766804 A CN 105766804A
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- 238000009395 breeding Methods 0.000 title claims abstract description 38
- 241000033688 Cotesia chilonis Species 0.000 title abstract 8
- 241000256844 Apis mellifera Species 0.000 claims abstract description 99
- 238000000034 method Methods 0.000 claims abstract description 38
- 230000001488 breeding effect Effects 0.000 claims abstract description 35
- 230000005058 diapause Effects 0.000 claims abstract description 31
- 210000004894 snout Anatomy 0.000 claims description 29
- 230000003071 parasitic effect Effects 0.000 claims description 23
- 238000005286 illumination Methods 0.000 claims description 14
- 241000033690 Cotesia ruficrus Species 0.000 claims description 13
- 230000008569 process Effects 0.000 claims description 12
- 230000024241 parasitism Effects 0.000 claims description 8
- 230000027326 copulation Effects 0.000 claims description 7
- 230000003203 everyday effect Effects 0.000 claims description 6
- 238000011081 inoculation Methods 0.000 claims description 4
- 241000256816 Braconidae Species 0.000 claims description 3
- 229920000742 Cotton Polymers 0.000 claims description 3
- 102000002322 Egg Proteins Human genes 0.000 claims description 3
- 108010000912 Egg Proteins Proteins 0.000 claims description 3
- 241000382353 Pupa Species 0.000 claims description 3
- 229920000297 Rayon Polymers 0.000 claims description 3
- 230000032669 eclosion Effects 0.000 claims description 3
- 210000004681 ovum Anatomy 0.000 claims description 3
- 239000002964 rayon Substances 0.000 claims description 3
- 230000015572 biosynthetic process Effects 0.000 claims description 2
- 230000027272 reproductive process Effects 0.000 claims description 2
- 239000000463 material Substances 0.000 abstract description 4
- 241001464311 Evergestis extimalis Species 0.000 abstract 1
- 241000257303 Hymenoptera Species 0.000 abstract 1
- 235000015097 nutrients Nutrition 0.000 abstract 1
- 241000894007 species Species 0.000 abstract 1
- 241000238631 Hexapoda Species 0.000 description 16
- 241000607479 Yersinia pestis Species 0.000 description 5
- 235000016709 nutrition Nutrition 0.000 description 5
- 230000035764 nutrition Effects 0.000 description 5
- 230000017448 oviposition Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 235000013601 eggs Nutrition 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000009825 accumulation Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000012447 hatching Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000011514 reflex Effects 0.000 description 2
- 241000426497 Chilo suppressalis Species 0.000 description 1
- 241000008892 Cnaphalocrocis patnalis Species 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241001147381 Helicoverpa armigera Species 0.000 description 1
- 206010023126 Jaundice Diseases 0.000 description 1
- 241000409991 Mythimna separata Species 0.000 description 1
- 241001249129 Scirpophaga incertulas Species 0.000 description 1
- 241000985245 Spodoptera litura Species 0.000 description 1
- 241000931752 Spodoptera mauritia Species 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000006399 behavior Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 210000004392 genitalia Anatomy 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000000266 injurious effect Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 231100000225 lethality Toxicity 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000001932 seasonal effect Effects 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 238000010200 validation analysis Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Feed For Specific Animals (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention relates to an artificial breeding method of cotesia chilonis matsumura.The method includes the steps of breeding hosts, prolonging the life of the species, breeding cotesia chilonis matsumura, delaying the propagation and storage of bred cotesia chilonis matsumura, and prolonging the life of host cotesia chilonis matsumura with Evergestis extimalis Scopoli larvae three to four years old as the intermediate hosts by means of external nutrients, thereby increasing the host search frequency of male and female cotesia chilonis matsumura.In addition, by means of the diapause of female cotesia chilonis matsumura, cotesia chilonis matsumura can be stored for a long time in the set environment; by means of lots of exploration, diapause is removed at any time for application under proper conditions.Materials for the method are easy to obtain, the breeding rate is high, stability is strong, and the problems that in an artificial transgenerational breeding method, the bee species is short in life and bees can hardly meet demands are solved.
Description
Technical field
The invention belongs to forestry pest technical field of biological control, be specifically related to the artificial fecundation method of a kind of Cotesia ruficrus.
Background technology
Along with the improvement of social development and economic technology condition, the Biological control of farm-forestry crop insect, comprehensive control are that to change traditional Natural control indispensable.The feature of Biological control is the developing direction utilizing natural enemy insect to be forest pest preventing and treating from now on, not only adapts to the growth requirement of new period forestry, also gradually forms theoretical system, and extensively admitted.
In the nearly stage, utilize the method for nature regulation and control, ecology etc. to become the trend of Harmful preventing and treating to prevent and treat woods insect, and utilize parasitic wasp to the important component part that the regulation and control of harmful insect population are also Harmful comprehensive controls.
The research of insect parasite is also relatively backward relative to the research of other agriculture and forestry injurious insect natural enemies for a long time.This is likely due to when selecting chemical agent and does not take into full account this medicament lethality to pest natural enemy, while controlling insect, high-efficiency low-toxicity need to be selected to avoid injury pest natural enemy and contaminated environment as far as possible.Additionally in natural contral, owing to the life-span of parasitic wasp is relatively short, greatly reduce the approach frequency of parasitic wasp and host, in addition, the generation of parasitic wasp has seasonality, and prior art cannot ensure the seriality of preventing and treating, high efficiency when insect is broken out in a large number, namely in insect peak period, cannot effectively discharge the parasitic wasp of indoor artificial propagation in quasi-ground on time, thus reaching the purpose of insect protected.
The artificial propagation of current cocoon honeybee faces host to be difficult to obtain, and the breeding of host does not have seriality；Exogenous nutrition is not good；Parasitic wasp is small, and inoculation technique difficulty is big, it is difficult to find suitable, propagating materials to carry out the problems such as breeding.
Summary of the invention
Present invention aim to overcome that the drawback that tradition subculture is bred, make Cotesia ruficrus can carry out annual accumulation and breed, there is seriality, solve worm honeybee not to lip reflex；And the present invention is by substantial amounts of Experimental Research, by selecting the exogenous nutrition of the best, extends the life-span of honeybee.Achieve the parasitic wasp of the release indoor artificial propagation on punctual quasi-ground, reach the purpose of real-time insect protected.
In order to solve above-mentioned four problems faced, the technical scheme is that the artificial fecundation method providing a kind of Cotesia ruficrus, what the method included host's cultivation, honeybee kind life-time dilatation, the inoculation of honeybee and diapause honeybee breeds the management process with honeybee；Wherein, with three to four open country, short comb angle in age snout moth's larva larvas for host, the hydromel of 15% is used to extend the parasitic wasp life-span, to increase female drone to New host records frequency；In 29 × 115mm flat based tubes, carry out numerous honeybee, adopt collection emergence interior female drone copulation in 2 hours and parasitism to be transferred to next flat based tubes after being not more than 2 hours.
In honeybee kind life-time dilatation process, temperature is preferably 28 ± 1 DEG C.
In honeybee kind life-time dilatation process, relative humidity is preferably (75.0 ± 5.0).
In honeybee kind life-time dilatation process, illumination is preferably 14L:10D.
Preferred scheme, in honeybee kind life-time dilatation process, collects and just sprouts wings, open country, healthy and of the same size short comb angle snout moth's larva braconid wasp, and every day feeds the hydromel 1 time of 15% with absorbent cotton.
Open country, host short comb angle snout moth's larva watch support carry out preferably at 24-30 DEG C of temperature.
Watching of open country, host short comb angle snout moth's larva is supported preferably in relative humidity as carrying out under (75.0 ± 5.0).
The watching to support when being 14L:10D preferably in illumination of open country, host short comb angle snout moth's larva carries out.
The worm raising open country, the host short comb angle snout moth's larva obtained is identical for age.
Preferred scheme, when the worm of host differs age, preserves the pupa of open country, short comb angle snout moth's larva at 12-14 DEG C, 15-25 days holding time；Or the ovum of open country, short comb angle snout moth's larva is preserved by individually/cooperation at 8-10 DEG C, 15-25 days holding time.
Preferred scheme, the seeded process of honeybee carries out at 24-30 DEG C, breeding bee tube accesses artificial breeding age consistent 3 open country, short comb angle in age snout moth's larva larvas, and press the honeybee worm of 1:5 than accessing the firm emergence queen bee through copulation, be placed horizontally in artificial temperature case after the sealing of breeding bee tube double gauze；After 2h, being drawn by queen bee, continuation accesses in the breeding bee tube placing larva and blade and carries out next round breeding；Again 25-29 DEG C, relative humidity raise for (75.0 ± 5.0) and to urge cocoon, produce honeybee kind.
Preferred scheme, in the reproductive process of diapause honeybee, by parasitic open country, short comb angle snout moth's larva, part being placed in 15-20 DEG C, illumination is 10-11 hour/day, intensity of illumination is 5000-7000Lx, until producing diapause bee source when relative humidity is (75.0 ± 5.0).
Preferred scheme, takes different management to the honeybee for breeding and diapause honeybee, after being used for the bee eclosion bred, dips, with rayon balls, the hydromel that concentration is 15% and raises；For the honeybee bred, being first depending on the phallic difference of female drone, distinguish male and female, then be divided in Tibetan honeybee pipe by sex ration 1:1 under Stereo microscope, queen bee is drawn and is used for breeding by post-coitum 2h；Diapause honeybee is kept separately at 2-8 DEG C and preserves.
Preferred scheme, diapause honeybee is termination of diapause at 24-29 DEG C.
Preferred scheme, the mean temperature of the formation of diapause honeybee is 16-19 DEG C, and every day, illumination length was 10-11 hour.
It is an advantage of the current invention that:
Open country, the host short comb angle snout moth's larva larva of the present invention, though a kind of solitary parasitism forestry pest, but have the advantage that host exists throughout the year in the present invention, easily obtain, easily cultivation, easily survive, easily breeding, generation overlap is obvious, and parasitic wasp mainly based on 3 age insect parasitic, make insect substantially still lose before gluttony in 4 ages and take food ability and dead, control population quantity and the harm in the present age of insect to a certain extent, and Cotesia ruficrus tool many hosts property, to mythimna separata, lawn armyworm, striped corn earworm and striped rice borer, yellow rice borer, rice leaf roller etc. also have and well control effect.
The present invention is before breeding, by substantial amounts of Experimental Research, dexterously working concentration be 15% hydromel feed, substantially prolongs the life-span of honeybee.
The present invention carries out numerous honeybee in 29 × 115mm flat based tubes, adopts collection just emergence interior female drone copulation in 2 hours and parasitism to be transferred to next flat based tubes in 2 hours.
Inventor is through lot of experiment validation, and the method can avoid the copulation under the time naturally allowed of female drone and the superparasitization behavior that honeybee kind is laid eggs, the efficient breeding of reliability with parasitic wasp to guarantee experiment.
Instant invention overcomes the drawback that tradition subculture is bred, make Cotesia ruficrus can carry out annual accumulation and breed, solve worm honeybee not to lip reflex, can according to demand termination of diapause at any time.
The present invention, without dissecting the genitals of honeybee to distinguish female drone, can distinguish by naked eyes.
The present invention is not more than 2 hours by restricting the parasitic wasp parasitism time, it is to avoid the superparasitization of parasitic wasp.
The present invention includes host's cultivation, honeybee kind life-time dilatation, educates breeding and preserving of honeybee step and diapause honeybee, with three to four open country, short comb angle in age snout moth's larva larvas for vector, utilizes exogenous nutrition to extend the life-span of parasitic wasp, thus increasing female drone to New host records frequency.
Additionally, the present invention utilizes the Diapause of female raw Cotesia ruficrus, it is possible not only to preserve for a long time under the environment that the present invention sets, and inventor is explored by a large amount of, termination of diapause and application at any time relatively suitable when.
The inventive method material is prone to obtain, and breeding potential is high, and stability is strong, solves the problem that the honeybee kind life-span in artificial subculture mating system is short and honeybee worm is difficult to boil on the nape opposite the mouth.
The present invention can endanger termination of diapause at any time according to the woodland of insect and control the outburst of insect.
Accompanying drawing explanation
Fig. 1 is the example of the indoor artificial propagation method flow chart of the raw Cotesia ruficrus of mother of the present invention.
Fig. 2 is that indoor same concentrations difference exogenous nutrition is to parasitic wasp life situation.
Fig. 3 is that variable concentrations hydromel is to parasitic wasp life situation.
Note: 5%, 10%, 15%, 20% represents the hydromel of variable concentrations respectively, 0 is experiment contrast group.
Illustrate the propagation method of the present invention below with embodiment, but the present invention is not limited to following embodiment.
Embodiment 1
Watching of open country, host short comb angle snout moth's larva is foster
At 24-30 DEG C of temperature, relative humidity is 14L:10D for (75.0 ± 5.0) illumination.Open country, the short comb angle snout moth's larva pieces of an egg taking 3-4d (being about to hatching) are some, are placed on fresh female leave sheet and wait to hatch；After hatching, fresh female leave sheet is added in timing every day, changes the blade that jaundice becomes withered in time, cultivates 8-9 days, and open country, short comb angle snout moth's larva larva length, to three ages, is ready to use in parasitic wasp parasitism.In the process for guaranteeing the concordance in worm age, the pupa of open country, short comb angle snout moth's larva can preserve at 12-14 DEG C, about 20 days holding times, and the ovum of open country, short comb angle snout moth's larva can preserve at 8-10 DEG C, about 20 days holding times.
Honeybee life-time dilatation
Being at 28 ± 1 DEG C of temperature in temperature, relative humidity is 14L:10D for (75.0 ± 5.0) illumination.Choose external source sugar 4 kinds common: respectively glucose, sucrose, fructose, Mel.Then it is diluted with distilled water into, by same ratio, the stand-by concentration that concentration is identical.Clear water process group is set simultaneously, using without feeding as comparison.Observe and record the life-span of honeybee, for exogenous nutrition distilled water, it is diluted to respectively 5%, 10%, 15% and 20% 4 Concentraton gradient with above-mentioned best carbohydrate (Mel).Collecting (in 2h) open country, healthy and of the same size short comb angle snout moth's larva braconid wasp of just sprouting wings, every day dips, with defat cotton swab, the concentration configured and feeds 1 time, with water for comparison, observes and record the life-span of honeybee.
The inoculation of honeybee
In 24-30 DEG C, 3 open country, short comb angle in the age snout moth's larva larvas that access artificial breeding age is consistent in breeding bee tube, and the honeybee worm ratio pressing 1:5 accesses a firm emergence (in 2h) through the queen bee of copulation, it is placed horizontally in artificial temperature case after breeding bee tube double gauze is sealed；After 2h, being drawn by queen bee, continuation accesses in the breeding bee tube placing larva and blade and carries out next round breeding；Again 25-29 DEG C, relative humidity raise for (75.0 ± 5.0) and to urge cocoon, produce honeybee kind；
The breeding of diapause bee source
Above-mentioned part is placed in 15-20 DEG C by parasitic open country, short comb angle snout moth's larva, illumination is 10-11 hour/day, intensity of illumination is 5000-8000Lx, relative humidity is under (75.0 ± 5.0) environment until producing diapause bee source, in nature, the cocoon that Cotesia ruficrus is formed has two types, and a kind of is pistac for developmental pattern, a kind of is diapause type, in yellowish-brown.
The management of honeybee: honeybee and diapause honeybee for breeding take different management
For, after the bee eclosion bred, dipping, with rayon balls, the hydromel that concentration is 15% and raise；For the honeybee bred, being first depending on the phallic difference of female drone, distinguish male and female, then be divided in Tibetan honeybee pipe by sex ration 1:1 under Stereo microscope, queen bee is drawn and is used for breeding by post-coitum 2h；Diapause honeybee be kept separately be placed under 2-8 DEG C of condition preserve, be 1 year, diapause honeybee can at 23-28 DEG C termination of diapause.
It is demonstrated experimentally that carry out the breeding of Cotesia ruficrus by the inventive method, parasitic material is easy to get, the honeybee life-span is extended, breeding potential is high, the cycle is short, population quantity is stable, be not subject to seasonal restrictions.
Table 1 for, after using the propagation method described in embodiment 1, breeding the cocoon honeybee quantity after generation and the contrast of the situation under natural conditions continuously from field acquisition to indoor:
Table 1
Note: above-mentioned 0th generation honeybee source, from field acquisition, by sex ration 1:1 mate and oviposit, is raised respectively under two conditions, the egg laying amount of single female egg laying amount and open country, parasitic wasp mean parasitized one short comb angle snout moth's larva.
Under natural environment, life-span and the egg laying amount of female raw Cotesia ruficrus are lower than general solitary parasitism cocoon honeybee, general female raw female drone average life 3d of Cotesia ruficrus, and the cocoon honeybee average life of solitary parasitism Spodoptera litura larvae is up to 5d, in its natural state, the single female reproductive frequency of the cocoon honeybee of the present invention is low, and single female eggs on average amount is few.But by the improvement of the present invention, egg laying amount, offspring's honeybee amount are greatly increased, and offspring's honeybee average life is greatly prolonged.
Table 2 cultivates diapause honeybee experimental result for method described in embodiment 1:
Table 2.
Claims (10)
1. the artificial fecundation method of a Cotesia ruficrus, it is characterised in that what the method included host's cultivation, honeybee kind life-time dilatation, the inoculation of honeybee and diapause honeybee breeds the management process with honeybee；Wherein, with three to four open country, short comb angle in age snout moth's larva larvas for host, the hydromel of 15% is used to extend the parasitic wasp life-span, to increase female drone to New host records frequency；In 29 × 115mm flat based tubes, carry out numerous honeybee, adopt collection emergence interior female drone copulation in 2 hours and parasitism to be transferred to next flat based tubes after being not more than 2 hours.
2. propagation method according to claim 1, it is characterised in that in honeybee kind life-time dilatation process, temperature is 28 ± 1 DEG C, and relative humidity is (75.0 ± 5.0), and illumination is 14L:10D.
3. propagation method according to claim 3, it is characterised in that in honeybee kind life-time dilatation process, collects and just sprouts wings, open country, healthy and of the same size short comb angle snout moth's larva braconid wasp, and every day feeds the hydromel 1 time of 15% with absorbent cotton.
4. propagation method according to claim 1, it is characterised in that watching of open country, host short comb angle snout moth's larva is supported 24-30 DEG C of temperature, and relative humidity is (75.0 ± 5.0), carries out when illumination is 14L:10D.
5. propagation method according to claim 4, it is characterised in that raise the worm of open country, host short comb angle snout moth's larva obtained identical for age, when the worm of host differs age, preserves the pupa of open country, short comb angle snout moth's larva at 12-14 DEG C, 15-25 days holding time；Or the ovum of open country, short comb angle snout moth's larva is preserved by individually/cooperation at 8-10 DEG C, 15-25 days holding time.
6. propagation method according to claim 1, it is characterized in that, the seeded process of honeybee carries out at 24-30 DEG C, 3 open country, short comb angle in the age snout moth's larva larvas that access artificial breeding age is consistent in breeding bee tube, and the honeybee worm ratio pressing 1:5 accesses the firm emergence queen bee through copulation, it is placed horizontally in artificial temperature case after breeding bee tube double gauze is sealed；After 2h, being drawn by queen bee, continuation accesses in the breeding bee tube placing larva and blade and carries out next round breeding；Again 25-29 DEG C, relative humidity raise for (75.0 ± 5.0) and to urge cocoon, produce honeybee kind.
7. propagation method according to claim 1, it is characterized in that, in the reproductive process of diapause honeybee, part is placed in 15-20 DEG C by parasitic open country, short comb angle snout moth's larva, illumination is 10-11 hour/day, intensity of illumination is 5000-7000Lx, until producing diapause bee source when relative humidity is (75.0 ± 5.0).
8. propagation method according to claim 1, it is characterised in that the honeybee for breeding and diapause honeybee are taked different management, after being used for the bee eclosion bred, dips, with rayon balls, the hydromel that concentration is 15% and raises；For the honeybee bred, being first depending on the phallic difference of female drone, distinguish male and female, then be divided in Tibetan honeybee pipe by sex ration 1:1 under Stereo microscope, queen bee is drawn and is used for breeding by post-coitum 2h；Diapause honeybee is kept separately at 2-8 DEG C and preserves.
9. propagation method according to claim 8, it is characterised in that diapause honeybee is termination of diapause at 24-29 DEG C.
10. the propagation method according to claim 1 or 8 or 9, it is characterised in that the mean temperature of the formation of diapause honeybee is 16-19 DEG C, and every day, illumination length was 10-11 hour.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107581159A (en) * | 2017-09-28 | 2018-01-16 | 沈阳农业大学 | A kind of figured cloth moths attracted by lamplight natural enemy hangs the artificial fecundation method of cocoon honeybee |
| CN109874752A (en) * | 2019-04-03 | 2019-06-14 | 中国农业科学院植物保护研究所 | A kind of diapause regulation of the endoparasitoid Microcentrus cingulum and storage method |
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| CN109874752B (en) * | 2019-04-03 | 2021-04-09 | 中国农业科学院植物保护研究所 | Diapause regulation and storage method for waistband long body hornets |
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