CN104996366B - A kind of Trichogramma efficient propagation method - Google Patents
A kind of Trichogramma efficient propagation method Download PDFInfo
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- CN104996366B CN104996366B CN201510470852.1A CN201510470852A CN104996366B CN 104996366 B CN104996366 B CN 104996366B CN 201510470852 A CN201510470852 A CN 201510470852A CN 104996366 B CN104996366 B CN 104996366B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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Abstract
A kind of snout moth's larva trichogramma efficient propagation method, collection rice moth egg, which is sticked on cardboard, is made ovum card；Trichogramma kind honeybee and rice moth egg card are pressed 1:10~14 ratio is placed in darkroom, and kind of a bee eclosion parasitic environment temperature is adjusted into 23-25 DEG C, humidity 70%-80%；After ovum card more than 90% is parasitic by honeybee kind, according to honeybee kind and ovum card 1:6~10 ratio adds fresh rice moth egg card again, and appropriate rise darkroom temperature is to 25-28 DEG C, and humidity is constant；After ovum card more than 90% is parasitic by honeybee kind, according to honeybee kind and ovum card 1:3~7 ratio third time to be added and spray sucrose nutrition liquid on fresh rice moth egg card, this deutovum card, continues to breed.The present invention can large area realize Trichogramma preventing and treating rice leaf roller and striped rice borer, pesticide dosage is greatly decreased, agricultural is promoted to green, organic, ecological agriculture development.
Description
Technical field
The invention belongs to rice disease and pest technical field of biological control, and in particular to a kind of efficient side of breeding of Trichogramma
Method.
Background technology
Trichogramma belongs to Hymenoptera, Trichogrammatidae, hobby move in rice field or similar palustrine in, can parasitic snout moth's larva
The worm's ovum of some insects such as moth section, Noctuidae, Hesperiidae, small Lycaenidae, Arctiidae, at the same be also striped rice borer, rice leaf roller,
The ovum phase natural enemy of the Insect Pests in Rice such as rice green caterpillar, rice plant skipper, has certain preventive and therapeutic effect to rice pest.
The Ministry of Agriculture proposes：To the year two thousand twenty, in terms of China's agricultural will realize " it is basic that a control two subtracts three ", agricultural chemicals, agriculture is promoted
Crop disease and insect Unifying control and green prevention and control, preferentially using Ecological Control, physical control and bio-control method, carry out low toxicity
The Demonstration And Extension of less-persistent pesticide, to the year two thousand twenty, staple crops pest and disease damage green prevention and control coverage rate reaches 30%, chemical pesticide
Usage amount realizes zero growth rate.In recent years, striped rice borer and rice leaf roller green prevention and control technology are prevented and treated at me using Trichogramma
The South Rice Region of China such as state Hunan, Jiangxi, Sichuan, Guangxi large area is successfully demonstrated, and greatly reduces the use of chemical pesticide.Hunan Province
Rice research, which is studied, to be shown, Trichogramma reaches respectively to the large area control effect of rice leaf roller and striped rice borer
69.34%-87.10% and 69.61%-77.29%, compared with chemical pesticide, preventive effect is suitable.
As this green prevention and control technology constantly expands in South Rice Region of China promoted extension, the demand to Trichogramma is got over
Come bigger, existing trichogramma reproduction technique and efficiency have been difficult to meet current market demand, seriously govern this technology more
The popularization and application of large area.The art of this patent is given birth on the basis that forefathers trichogramma cultivates with reference to the physiology of Trichogramma
Long characteristic, it is proposed that be suitable for the parasitic dark surrounds of Trichogramma breeding, improve the in batches parasitic method of breeding coefficient, and
The nutrient solution of bee hatching rate and eclosion rate is improved, the breeding coefficient and honeybee kind quality of Trichogramma is effectively increased, can be paddy rice
The large scale application of green cultivation technique is taken a firm foundation.
The content of the invention
The technical problems to be solved by the invention are：For asking that chemical agent in south rice production process is largely used
Topic, and there is provided a kind of Trichogramma efficient propagation method, realize that Trichogramma prevents and treats rice leaf roller to large area
With the purpose of striped rice borer, be greatly decreased South Rice Region of China chemical pesticide consumption, promote China's agricultural to green agriculture, organic agriculture,
The development of the ecological agriculture.
The embodiment of this technology is：Rice moth egg is collected to be sticked on cardboard and ovum card is made；By Trichogramma kind
Honeybee presses 1 with rice moth egg card:10~14 ratio is placed in darkroom, and kind of a bee eclosion parasitic environment temperature is adjusted into 23-25 DEG C, humidity
70%-80%；After ovum card more than 90% is parasitic by honeybee kind, according to honeybee kind and ovum card 1:6~10 ratio is added fresh again
Rice moth egg card, appropriate rise darkroom temperature is to 25-28 DEG C, and humidity is constant；After ovum card more than 90% is parasitic by honeybee kind, according to honeybee
Plant and ovum card 1:3~7 ratio third time to be added and spray sucrose nutrition liquid on fresh rice moth egg card, this deutovum card, continues to breed.
In above-mentioned technical proposal, the sucrose nutrition liquid is 5% sucrose nutrition liquid.
Compared with prior art, beneficial effects of the present invention are：
First, Trichogramma illumination and it is dark two kinds in the case of can parasitic rice moth egg, prior art is typically to exist
It is parasitic under illumination condition, the present invention by experimental studies have found that, under dark condition, the life cycle of Trichogramma, which slightly has, prolongs
Long, the parasitic effects to rice moth egg are better than illumination condition, can greatly improve the fertility of Trichogramma.
Second, the method that fresh rice moth egg card successively decreases by quantity is allowed Trichogramma honeybee kind parasitic by the present invention in three times,
Compared with existing disposable Parasite technology, parasitism can guarantee that the freshness of rice moth egg by several times, so that rice moth egg is easier
It is parasitic.
3rd, the parasitic cycle of existing trichogramma reproduction technique is short, can complete whole parasitic processes within general 2-3 days, because
This, the nutritional supplementation in parasitic processes is not affected by attention always.The present invention passes through parasitic by parasitic cycle stretch-out by several times, it is ensured that
The parasitic ability of later stage Trichogramma, sprays 5% sucrose nutrition liquid on honeybee card and ovum card, can improve the work of Trichogramma
Power, so as to further improve the breeding coefficient of Trichogramma.
Test effect：
It can be seen from upper table, the result of the test on May 14th, 2015 and June 4 shows, with the existing breeding of Trichogramma
Technology is compared, and the present invention can effectively improve the breeding coefficient of Trichogramma, and breeding coefficient reaches as high as 1:27.9.
Embodiment
Embodiment 1：
Collect rice moth egg and ovum card is made；Trichogramma honeybee kind and ovum card are pressed 1:12 ratio is placed in darkroom, and will
Darkroom temperature is adjusted to 23 DEG C, humidity 70%；After ovum card more than 90% is parasitic by honeybee kind, according to honeybee kind and ovum card 1:8 ratio
Fresh ovum card is added, appropriate rise darkroom temperature is to 25 DEG C, and humidity is constant；After ovum card more than 90% is parasitic by honeybee kind, according to
Honeybee kind and ovum card 1:5 ratio to be added spray 5% sucrose nutrition liquid on fresh ovum card, this deutovum card again, continues to breed.
Embodiment 2：
Collect rice moth egg and ovum card is made；Trichogramma honeybee kind and ovum card are pressed 1:10 ratio is placed in darkroom, and will
Darkroom temperature is adjusted to 24 DEG C, humidity 75%；After ovum card more than 90% is parasitic by honeybee kind, according to honeybee kind and ovum card 1:6 ratio
Fresh ovum card is added, appropriate rise darkroom temperature is to 26 DEG C, and humidity is constant；After ovum card more than 90% is parasitic by honeybee kind, according to
Honeybee kind and ovum card 1:3 ratio to be added spray 5% sucrose nutrition liquid on fresh ovum card, this deutovum card again, continues to breed.
Embodiment 3：
Collect rice moth egg and ovum card is made；Trichogramma honeybee kind and ovum card are pressed 1:14 ratio is placed in darkroom, and will
Darkroom temperature is adjusted to 25 DEG C, humidity 80%；After ovum card more than 90% is parasitic by honeybee kind, according to honeybee kind and ovum card 1:10 ratio
Fresh ovum card is added, appropriate rise darkroom temperature is to 28 DEG C, and humidity is constant；After ovum card more than 90% is parasitic by honeybee kind, according to
Honeybee kind and ovum card 1:7 ratio to be added spray 5% sucrose nutrition liquid on fresh ovum card, this deutovum card again, continues to breed.
Claims (2)
1. a kind of snout moth's larva trichogramma efficient propagation method, it is characterised in that collect rice moth egg and sticked on cardboard and ovum card is made；
Trichogramma kind honeybee and rice moth egg card are pressed 1:10~14 ratio is placed in darkroom, and will plant bee eclosion parasitic environment temperature tune
To 23-25 DEG C, humidity 70%-80%；After ovum card more than 90% is parasitic by honeybee kind, according to honeybee kind and ovum card 1:6~10 ratio
Example adds fresh rice moth egg card again, and appropriate rise darkroom temperature is to 25-28 DEG C, and humidity is constant；Treat ovum card more than 90% by honeybee
Plant after parasitism, according to honeybee kind and ovum card 1:3~7 ratio third time to be added and spray sucrose on fresh rice moth egg card, this deutovum card
Nutrient solution, continues to breed.
2. snout moth's larva trichogramma efficient propagation method according to claim 1, it is characterised in that the sucrose nutrition liquid is 5%
Sucrose nutrition liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510470852.1A CN104996366B (en) | 2015-08-04 | 2015-08-04 | A kind of Trichogramma efficient propagation method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510470852.1A CN104996366B (en) | 2015-08-04 | 2015-08-04 | A kind of Trichogramma efficient propagation method |
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| Publication Number | Publication Date |
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| CN104996366A CN104996366A (en) | 2015-10-28 |
| CN104996366B true CN104996366B (en) | 2017-07-14 |
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| CN201510470852.1A Active CN104996366B (en) | 2015-08-04 | 2015-08-04 | A kind of Trichogramma efficient propagation method |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106689063B (en) * | 2016-12-07 | 2020-03-17 | 中国科学院动物研究所 | Artificial breeding production method of trichogramma canker of rice borer |
| CN108056070B (en) * | 2018-01-16 | 2021-11-12 | 吉林农业大学 | Method for breeding trichogramma by using snowflake moth |
| CN111011313B (en) * | 2019-12-30 | 2022-04-19 | 中国医学科学院药用植物研究所云南分所 | Method for improving female proportion of trichogramma offspring and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1647626A (en) * | 2005-01-26 | 2005-08-03 | 北京市农林科学院 | Artificial breeding and producing method for trichogramma |
| CN101438691A (en) * | 2007-11-23 | 2009-05-27 | 北京市农林科学院 | Mass propagation method of Oophagous trichogrammae from artificial ovum |
| CN102106319A (en) * | 2011-01-18 | 2011-06-29 | 西北农林科技大学 | Method for propagating trichogramma pintoi voegele indoors by grapholitha molesta busck egg |
-
2015
- 2015-08-04 CN CN201510470852.1A patent/CN104996366B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1647626A (en) * | 2005-01-26 | 2005-08-03 | 北京市农林科学院 | Artificial breeding and producing method for trichogramma |
| CN101438691A (en) * | 2007-11-23 | 2009-05-27 | 北京市农林科学院 | Mass propagation method of Oophagous trichogrammae from artificial ovum |
| CN102106319A (en) * | 2011-01-18 | 2011-06-29 | 西北农林科技大学 | Method for propagating trichogramma pintoi voegele indoors by grapholitha molesta busck egg |
Non-Patent Citations (2)
| Title |
|---|
| "中间寄主对甘蓝夜蛾赤眼蜂寄生行为的影响";王卫华 等;《植物保护》;20030430;第29卷(第2期);第23-25页 * |
| "以米蛾卵为寄主繁殖玉米螟赤眼蜂的质量控制技术";陈红印 等;《昆虫天敌》;20001231;第22卷(第4期);第145-150页 * |
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| CN104996366A (en) | 2015-10-28 |
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