CN101438691A - Mass propagation method of Oophagous trichogrammae from artificial ovum - Google Patents
Mass propagation method of Oophagous trichogrammae from artificial ovum Download PDFInfo
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- CN101438691A CN101438691A CNA2007101873683A CN200710187368A CN101438691A CN 101438691 A CN101438691 A CN 101438691A CN A2007101873683 A CNA2007101873683 A CN A2007101873683A CN 200710187368 A CN200710187368 A CN 200710187368A CN 101438691 A CN101438691 A CN 101438691A
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Abstract
The invention relates to a mass reproduction method of an oophagous trichogrammae by artificial eggs. The method comprises the following three steps: purifying parent oophagous trichogrammae after a plurality of reproductions of the oophagous trichogrammae; injecting a prepared artificial egg solution into the artificial eggs; and inoculating the parent oophagous trichogrammae into the artificial eggs to reproduce the oophagous trichogrammae. The method can be used in large scale industrialized production, and has simple processes and year-round production, which greatly saves labor and materials, and lowers the cost.
Description
Technical field
The present invention relates to the method for a large amount of breeding oophagous trichogrammaes of a kind of artificial host egg.
Background technology
The nature parasitic bee is with a long history in China, the existing many families of its numerous honeybee rules and target level of product quality formulate, before a large amount of numerous honeybees, at first to breed vector-insect ovum in a large number, what be most widely used at present is to utilize egg of oak silkworm to carry out a large amount of indoor propagations, expend a large amount of human and material resources, in a large amount of production applications, be subjected to the restriction of factors such as time, region, transportation.
In the research process of artificial ovum oophagous trichogrammae, many countries began just in research oophagous trichogrammae extracorporeal culture technology from the seventies.At first short tube oophagous trichogrammae (Trichogramma pretiosum) in-vitro breeding is successful in 1975 with cotton bollworm 5 instar larvae hemolymphs by American Hoffman.And then Switzerland's Ciba-Geigy company invests 2,500,000 dollars the eighties and cooperates to carry out at Weslaco " no insect material " artificial ovum breeding oophagous trichogrammae research with the United States Department of Agriculture.Because the raw material costliness, tool reality production meaning is not abandoned this research in nineteen ninety-five.In addition, states such as France, Australia, Brazil, Korea S, Japan, Thailand, Russia, Canada are also all carrying out this research, but also just rest on laboratory stage, do not set up production line as yet, and actual production and application are not arranged.
Summary of the invention
In order to overcome the defective of nature parasitic bee, the invention provides a kind of mass propagation method of Oophagous trichogrammae from artificial ovum, it can carry out large-scale industrial production, has reduced cost.
For solving the problems of the technologies described above, a kind of mass propagation method of Oophagous trichogrammae from artificial ovum of the present invention comprises the steps:
(1) parasitism is had the insect ovum of oophagous trichogrammae place under 23 ℃～27 ℃, the condition of humidity 65%～75% and grows, sprout wings honeybee after, be transferred in the vector ovum, bred for 1～3 generation continuously after impurity elimination purify, receive artificial ovum again and carry out 1-4 for the domestication breeding;
(2) make artificial ovum liquid in the ratio of egg 13～15%, milk powder 25～27%, tussah chrysalis homogenate 30～35% and water 25～30% after, add antibiotic, antibiotic concentration is 180～220mg/L; After injecting the artificial ovum liquid of 5～6 microlitres in every artificial ovum, with sealing around the artificial ovum card;
(3) carry out numerous honeybee in honeybee, ovum ratio for the ratio of 3-5:1, each numerous honeybee is changed 2-4 fresh ovum card; Every batch connects the honeybee time is 6-10 hour; Treat after numerous honeybee is finished the something lost honeybee on ovum surface to be disposed, have the artificial ovum card of oophagous trichogrammae to send into the growth chamber parasitism and grow; Grow 23～27 ℃ of indoor maintenance temperature, humidity 65～75%.
As a kind of improvement of the present invention, add the formaldehyde of 5-8 gram potassium permanganate and equivalent before numerous honeybee by every cubic metre of container, oophagous trichogrammae kind honeybee is carried out fumigation.
Further improve as the present invention, described artificial ovum card employing thickness is 0.25-0.4 micron the poly-third poly-second composite membrane, and every artificial ovum card has at least one artificial ovum recess.
Preferably, have the insect ovum of oophagous trichogrammae to place under the condition of 25 ℃ of temperature, humidity 70% parasitism and grow, sprout wings honeybee after, be transferred in the vector ovum, bred for 3 generations continuously after impurity elimination purify, receive artificial ovum again and carry out 3-4 for the domestication breeding.
Preferably, prepare artificial ovum liquid in the ratio of egg 14%, milk powder 26%, tussah chrysalis homogenate 32～35% and water 27～30%.
Preferably, than carrying out numerous honeybee for the ratio of 4:1, each numerous honeybee is changed 3-4 fresh ovum card in honeybee, ovum; Every batch connects the honeybee time is 8-10 hour.
Preferably, grow 25 ℃ of indoor maintenance temperature, humidity 70%.
The artificial ovum mass propagation method of Oophagous trichogrammae of the present invention has following technological merit: can carry out large-scale industrial production, program is simple, but and produce in the anniversary, saved the man power and material greatly, reduced cost.
Embodiment
A kind of mass propagation method of Oophagous trichogrammae from artificial ovum of the present invention comprises the steps:
(1) parasitism is had the insect ovum of oophagous trichogrammae place under 23 ℃～27 ℃, the condition of humidity 65%～75% and grows, sprout wings honeybee after, be transferred in the vector ovum, bred for 1～3 generation continuously after impurity elimination purify, receive artificial ovum again and carry out 1-4 for the domestication breeding;
(2) make artificial ovum liquid in the ratio of egg 13～15%, milk powder 25～27%, tussah chrysalis homogenate 30～35% and water 25～30% after, add antibiotic, antibiotic concentration is 180～220mg/L;
After injecting the artificial ovum liquid of 5～6 microlitres in each artificial ovum, with sealing around the artificial ovum card;
(3) carry out numerous honeybee in honeybee, ovum ratio for the ratio of 3-5:1, each numerous honeybee is changed 2-4 fresh ovum card; Every batch connects the honeybee time is 6-10 hour; Treat after numerous honeybee is finished the something lost honeybee on ovum surface to be disposed, have the artificial ovum card of oophagous trichogrammae to send into the growth chamber parasitism and grow; Grow 23～27 ℃ of indoor maintenance temperature, humidity 65～75%.
Embodiment 1
(1) planting honeybee purifies
Have the insect ovum of oophagous trichogrammae to place under 23 ℃, the condition of humidity 65% parasitism and grow, sprout wings honeybee after, be transferred in the vector rice moth egg, bred for 1 generation continuously after impurity elimination purify, determine the good species honeybee.With the good species honeybee of purification and rejuvenation, carry out the domestication breeding of 1 generation with artificial ovum, make that parasitic rate, eclosion rate, fertility, property compare, the life-span is suitable with natural egg parasite.
(2) artificial ovum card is produced
Prepare artificial ovum liquid by 13% egg, 25% milk powder, 32% tussah chrysalis homogenate and 30% water, every milliliter of ovum liquid adds the antibiotic of 180 micrograms.
Artificial ovum card adopts the poly-third poly-second composite membrane of 0.25 micron thickness.Every artificial ovum card has 10 artificial ovum recesses, after injecting the artificial ovum liquid of 5 microlitres in each artificial ovum, with sealing around the artificial ovum card.
(3) the commodity honeybee produces
Before numerous honeybee, will plant honeybee and be placed in the container, add 5 gram potassium permanganate and 5 milliliters of formaldehyde, oophagous trichogrammae kind honeybee will be carried out fumigation by every cubic metre.
Adopting glass infuser to carry out numerous honeybee in honeybee, ovum than the ratio for 3:1, obturages with black cloth in honeybee tube two ends.Replaceable 2 the fresh ovum cards of each numerous honeybee, every batch connects the honeybee time is 6 hours.Treat after whole numerous honeybees are finished the something lost honeybee on ovum surface to be disposed, have the artificial ovum card of oophagous trichogrammae to send into the growth chamber parasitism and grow.Grow 23 ℃ of indoor maintenance temperature, humidity 65%.
(4) the commodity honeybee stores
When artificial ovum endoparasitism honeybee grows to mature larva during the phase, the timely freezer stored for future use of 5-8 ℃ of shift-in temperature, humidity 55%-65%.Guarantee temperature, moisture stable in the freezer, not with poisonous, dangerous things mixed storage.
Embodiment 2
Comparing with embodiment 1, only is that above-mentioned steps (1) changes, and different is: have the insect ovum of oophagous trichogrammae to place under 25 ℃, the condition of humidity 70% parasitism and grow, sprout wings honeybee after, be transferred in the vector rice moth egg, breed after 2 generations impurity elimination continuously and purify, determine the good species honeybee.With the good species honeybee of purification and rejuvenation, carry out the domestication breeding of 3 generations with artificial ovum, make that parasitic rate, eclosion rate, fertility, property compare, the life-span is suitable with natural egg parasite.
Embodiment 3
Comparing with embodiment 2, only is that step (2) changes, and different is: prepare artificial ovum liquid by 15% egg, 27% milk powder, 30% tussah chrysalis homogenate and 28% water, every milliliter of ovum liquid adds the antibiotic of 200 micrograms.
Artificial ovum card adopts the poly-third poly-second composite membrane of 0.32 micron thickness.Every artificial ovum card has 80 artificial ovum recesses, inject the artificial ovum liquid of 5 microlitres in every artificial ovum after, will artificial ovum card around sealing.
Embodiment 4
Comparing with embodiment 3, only is that step (3) changes, and different is: will plant honeybee and be placed in the container before numerous honeybee, and add 7 gram potassium permanganate and 7 milliliters of formaldehyde by every cubic metre, oophagous trichogrammae kind honeybee is carried out fumigation.
Adopting glass infuser to carry out numerous honeybee in honeybee, ovum than the ratio for 4:1, obturages with black cloth in honeybee tube two ends.Replaceable 3 the fresh ovum cards of each numerous honeybee, every batch connects the honeybee time is 8 hours.Treat after whole numerous honeybees are finished the something lost honeybee on ovum surface to be disposed, have the artificial ovum card of oophagous trichogrammae to send into the growth chamber parasitism and grow.Grow 25 ℃ of indoor maintenance temperature, humidity 70%.
Embodiment 5
Comparing with embodiment 1, only is that step (1) changes, and different is: have the insect ovum of oophagous trichogrammae to place under 24 ℃, the condition of humidity 75% parasitism and grow, sprout wings honeybee after, be transferred in the vector egg of oak silkworm, breed after 3 generations impurity elimination continuously and purify, determine the good species honeybee.With the good species honeybee of purification and rejuvenation, carry out the domestication breeding of 4 generations with artificial ovum, make that parasitic rate, eclosion rate, fertility, property compare, the life-span is suitable with natural egg parasite.
Embodiment 6
Comparing with embodiment 5, only is that step (2) changes, and different is: prepare artificial ovum liquid by 14% egg, 26% milk powder, 35% tussah chrysalis homogenate and 25% water, every milliliter of ovum liquid adds the antibiotic of 220 micrograms.
Artificial ovum card adopts the poly-third poly-second composite membrane of 0.4 micron thickness.Every artificial ovum card has 140 artificial ovum recesses, after injecting the artificial ovum liquid of 6 microlitres in each artificial ovum, with sealing around the artificial ovum card.
Embodiment 7
Comparing with embodiment 6, only is that step (3) changes, and different is: will plant honeybee and be placed in the container before numerous honeybee, and add 8 gram potassium permanganate and 8 milliliters of formaldehyde by every cubic metre, oophagous trichogrammae kind honeybee is carried out fumigation.
Adopt aluminum alloy frame to carry out numerous honeybee in honeybee, ovum than ratio for 5:1.Replaceable 4 the fresh ovum cards of each numerous honeybee, every batch connects the honeybee time is 10 hours.Treat after whole numerous honeybees are finished the something lost honeybee on ovum surface to be disposed, have the artificial ovum card of oophagous trichogrammae to send into the growth chamber parasitism and grow.Grow 27 ℃ of indoor maintenance temperature, humidity 75%.
The testing result of the oophagous trichogrammae quality that the artificial ovum mass propagation method of Oophagous trichogrammae of the present invention is produced is as follows:
This method can be carried out large-scale industrial production and large-area applications.Oophagous trichogrammae with this method breeding can be widely used in the rural market, in order to agriculture primary pests such as control of maize snout moth's larva, cotton bollworm, soybean pod borer, lima bean pod borers.
Claims (7)
1, a kind of mass propagation method of Oophagous trichogrammae from artificial ovum comprises the steps:
(1) parasitism is had the insect ovum of oophagous trichogrammae place under the suitable temperature and humidity condition and grows, sprout wings honeybee after, be transferred in the vector ovum, bred for 1～3 generation continuously after impurity elimination purify, receive artificial ovum again and carry out 1-4 for the domestication breeding;
(2) make artificial ovum liquid in the ratio of egg 13～15%, milk powder 25～27%, tussah chrysalis homogenate 30～35% and water 25～30% after, add antibiotic, antibiotic concentration is 180～220mg/L, inject the artificial ovum liquid of 5～6 microlitres in each artificial ovum after, will artificial ovum card around sealing;
(3) carry out numerous honeybee in honeybee, ovum ratio for the ratio of 3-5:1, each numerous honeybee is changed 2-4 fresh ovum card; Every batch connects the honeybee time is 6-10 hour; Treat after numerous honeybee is finished the something lost honeybee on ovum surface to be disposed, have the artificial ovum card of oophagous trichogrammae to send into the growth chamber parasitism and grow; Grow the suitable temperature and humidity of indoor maintenance.
2, mass propagation method of Oophagous trichogrammae from artificial ovum according to claim 1 is characterized in that: add the formaldehyde of 5-8 gram potassium permanganate and equivalent before numerous honeybee by every cubic metre of container, oophagous trichogrammae kind honeybee is carried out fumigation.
3, mass propagation method of Oophagous trichogrammae from artificial ovum according to claim 2 is characterized in that: described artificial ovum card employing thickness is 0.25-0.4 micron the poly-third poly-second composite membrane, and every artificial ovum card has at least one artificial ovum recess.
4, mass propagation method of Oophagous trichogrammae from artificial ovum according to claim 1, it is characterized in that: have the insect ovum of oophagous trichogrammae to place under the condition of 25 ℃ of temperature, humidity 70% parasitism and grow, sprout wings honeybee after, be transferred in the vector ovum, impurity elimination is purified after breeding for 3 generations continuously, receives artificial ovum again and carries out 3-4 for the domestication breeding.
5, mass propagation method of Oophagous trichogrammae from artificial ovum according to claim 4 is characterized in that: prepare artificial ovum liquid in the ratio of egg 14%, milk powder 26%, tussah chrysalis homogenate 32～35% and water 27～30%.
6, mass propagation method of Oophagous trichogrammae from artificial ovum according to claim 5 is characterized in that: than carrying out numerous honeybee for the ratio of 4:1, each numerous honeybee is changed 3-4 fresh ovum card in honeybee, ovum; Every batch connects the honeybee time is 8-10 hour.
7, mass propagation method of Oophagous trichogrammae from artificial ovum according to claim 6 is characterized in that: grow 25 ℃ of indoor maintenance temperature, humidity 70%.
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| CNA2007101873683A CN101438691A (en) | 2007-11-23 | 2007-11-23 | Mass propagation method of Oophagous trichogrammae from artificial ovum |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102524112A (en) * | 2012-02-29 | 2012-07-04 | 广东省农业科学院植物保护研究所 | Device and method for breeding Trichogramma |
| CN102823550A (en) * | 2012-09-19 | 2012-12-19 | 吉林农业大学 | Light environment control method for breeding trichogramma dendrolimi with oak silkworm eggs |
| CN103503836A (en) * | 2013-08-22 | 2014-01-15 | 北京市农林科学院 | Plutella xylostella cotesia rubecula artificial propagation production method |
| CN103782969A (en) * | 2014-03-03 | 2014-05-14 | 广西壮族自治区林业科学研究院 | Method for breeding trichogramma pintoi indoors and corcyra cephalonica raising fodder |
| CN104255660A (en) * | 2014-09-01 | 2015-01-07 | 吉林农业大学 | Method for alternately storing trichogramma at intermediate and low temperature in two steps |
| CN104996366A (en) * | 2015-08-04 | 2015-10-28 | 湖南省水稻研究所 | Trichogramma japonicum high-efficiency reproduction method |
| CN105123353A (en) * | 2015-08-30 | 2015-12-09 | 常州思宇环保材料科技有限公司 | Biological rice disease and pest prevention system design |
| CN109169532A (en) * | 2018-10-11 | 2019-01-11 | 吉林农业大学 | Egg of oak silkworm is the method that host domestication breeds Trichogramma Ostriniae |
| CN109169531A (en) * | 2018-10-11 | 2019-01-11 | 吉林农业大学 | Armyworm trichogramma domestication mating system and control and application suitable for eating-core bean worm prevention and treatment |
| CN112119980A (en) * | 2020-08-31 | 2020-12-25 | 广东省科学院动物研究所 | Low-temperature propagation method for in vitro culture of trichogramma |
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2007
- 2007-11-23 CN CNA2007101873683A patent/CN101438691A/en active Pending
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102524112A (en) * | 2012-02-29 | 2012-07-04 | 广东省农业科学院植物保护研究所 | Device and method for breeding Trichogramma |
| CN102823550A (en) * | 2012-09-19 | 2012-12-19 | 吉林农业大学 | Light environment control method for breeding trichogramma dendrolimi with oak silkworm eggs |
| CN103503836A (en) * | 2013-08-22 | 2014-01-15 | 北京市农林科学院 | Plutella xylostella cotesia rubecula artificial propagation production method |
| CN103782969A (en) * | 2014-03-03 | 2014-05-14 | 广西壮族自治区林业科学研究院 | Method for breeding trichogramma pintoi indoors and corcyra cephalonica raising fodder |
| CN103782969B (en) * | 2014-03-03 | 2015-08-19 | 广西壮族自治区林业科学研究院 | A kind of method of indoor propagation Trichogramma pintoi and rice moth culturing feed |
| CN104255660A (en) * | 2014-09-01 | 2015-01-07 | 吉林农业大学 | Method for alternately storing trichogramma at intermediate and low temperature in two steps |
| CN104996366A (en) * | 2015-08-04 | 2015-10-28 | 湖南省水稻研究所 | Trichogramma japonicum high-efficiency reproduction method |
| CN104996366B (en) * | 2015-08-04 | 2017-07-14 | 湖南省水稻研究所 | A kind of Trichogramma efficient propagation method |
| CN105123353A (en) * | 2015-08-30 | 2015-12-09 | 常州思宇环保材料科技有限公司 | Biological rice disease and pest prevention system design |
| CN109169532A (en) * | 2018-10-11 | 2019-01-11 | 吉林农业大学 | Egg of oak silkworm is the method that host domestication breeds Trichogramma Ostriniae |
| CN109169531A (en) * | 2018-10-11 | 2019-01-11 | 吉林农业大学 | Armyworm trichogramma domestication mating system and control and application suitable for eating-core bean worm prevention and treatment |
| CN109169531B (en) * | 2018-10-11 | 2021-10-26 | 吉林农业大学 | Domestication breeding method and application of trichogramma armeniaca suitable for prevention and treatment of soybean pod borers |
| CN109169532B (en) * | 2018-10-11 | 2021-10-29 | 吉林农业大学 | Method for artificially domesticating and breeding corn borer trichogramma by taking tussah eggs as hosts |
| CN112119980A (en) * | 2020-08-31 | 2020-12-25 | 广东省科学院动物研究所 | Low-temperature propagation method for in vitro culture of trichogramma |
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Open date: 20090527 |