CN102144621A - Induced scleroderma guani breeding method - Google Patents
Induced scleroderma guani breeding method Download PDFInfo
- Publication number
- CN102144621A CN102144621A CN 201110102239 CN201110102239A CN102144621A CN 102144621 A CN102144621 A CN 102144621A CN 201110102239 CN201110102239 CN 201110102239 CN 201110102239 A CN201110102239 A CN 201110102239A CN 102144621 A CN102144621 A CN 102144621A
- Authority
- CN
- China
- Prior art keywords
- host
- honeybee
- substitute
- hosts
- low temperature
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention concretely relates to an induced scleroderma guani breeding method, wherein the problems that when the conventional scleroderma guani breeds, the obtainment of substitute hosts is limited by time and the heavy demand of the scleroderma guani on production cannot be satisfied because of limited sources are solved. The induced scleroderma guani breeding method comprises the following steps of: a, buying breeding scleroderma guani and preserving at low temperature; b, buying inducing hosts and preserving at low temperature; c, buying the substitute hosts and carrying out chemical coma and then processing at low temperature, wherein the substitute hosts are first hatched pupae of barley pests; d, inoculating the scleroderma guani on the inducing hosts to carry out induced spawning; e, putting the substitute hosts and culturing; and f, storing at low temperature and transporting. By means of the induced scleroderma guani breeding method provided by the invention, the problems that when the conventional scleroderma guani breeds, the obtainment of the substitute hosts is limited by time and the heavy demand of the scleroderma guani on production cannot be satisfied because of the limited sources are solved; the breeding yield of the scleroderma guani can be greatly increased through the method of culturing the scleroderma guani by utilizing the substitute hosts after the induced spawning by utilizing the inducing hosts; and in the meantime, the influences of cost and time limits when the substitute hosts are selected are reduced.
Description
Technical field
The present invention relates to the mating system of natural enemy, relate in particular to the mating system of swollen leg honeybee, be specially the swollen leg honeybee mating system of a kind of induction type.
Background technology
Trunk borer is the important worm kind of in the forestry pest, and annual China's forest ecological environment of all can giving causes huge economy and ecological loss.Propagated by Monochamus alternatus as pine nematode, in a single day pine tree catches an illness and just can't cure, and China has added up dead pine tree several hundred million strains because of infecting pine nematode, and economic loss is above 100,000,000,000 yuan; Red turpentine beetle also is a kind of trunk borer, has caused nearly ten million strain Chinese pine death; China's willow planting area reaches 100,000,000 mu, and is also more than other national willow planting area summation of the world, based on the trunk borer of anoplophora glabripennis willow caused grave danger, area takes place every year up to 9,000,000 mu; Trunk borer harm such as red-edge longicorn, semanotus bifasciatus, mountain longicorn, batocera horsfieldi are also very big in addition.
The control of trunk borer is world-famous puzzle always, because trunk borer is sought hidden life, the most of the time all bores moth and endangers in pin main body in its whole life, so general chemical control poor effect.A large amount of simultaneously use chemical pesticides also can cause serious pollution and destruction to ecotope.
Utilize natural enemy control trunk borer have with worm control worm, biotic population internal balance, free from environmental pollution, can be for a long time, the advantage of lasting, stable control disaster, discharge a day enemy's rear area, natural enemy can be sought also attack trunk borer automatically, control efficiency is fine.Production is at present gone up trunk borer natural enemy commonly used and is swollen leg honeybee, generally adopts Cathay poplar longicorn pupa to be alternative host and breeds the control that swollen leg honeybee is used for trunk borer.Yet because the source of Cathay poplar longicorn pupa is limited, it is higher to obtain cost, can't satisfy and produce the wilderness demand of going up swollen leg honeybee.Simultaneously, the annual time basic fixed that obtains Cathay poplar longicorn pupa, usually the best period with the control trunk borer misfits, and causes control efficiency undesirable, also can miss Best Times sometimes and can't adopt natural enemy to prevent and treat.
Summary of the invention
The present invention for solve substitute when existing swollen leg honeybee breeds the host obtain be subjected to time restriction, source limited can't satisfy produce the problem that goes up swollen leg honeybee wilderness demand, a kind of induction type leg honeybee mating system that swells is provided.
The present invention adopts following technical scheme to realize: the induction type leg honeybee mating system that swell, and step is as follows: a, buy swollen leg honeybee kind honeybee low temperature preservation; B, buy and induce host's low temperature to preserve; C, buy and substitute the host and carry out chemistry stupor back low temperature treatment, described alternative host is the first pupa of incubating of barley worm; D, the leg honeybee of will swelling are seeded in to induce and carry out induced spawning on the host; E, put into and cultivate after substituting the host; Transport behind f, the cryopreservation.That induces the host is chosen for that those skilled in the art realize easily.The leg honeybee of will swelling earlier in this method inoculates the back induced spawning with inducing the host, put into then and substitute host's cultivation, bee yield is significantly improved, impel alternative host to lie in a comatose condition fast by chemistry stupor mode simultaneously, help swollen leg larva of bee to get food, further improved bee yield, solved the easy epidermis of alternative host simultaneously and hardened easily and influence the problem of culture effect; Substituting choosing through a large amount of repetition tests of host draws.
Inducing the host is that bumblebee just incubates pupa, adopts bumblebee just to incubate pupa and does and induce the host to induce the swollen leg honeybee effect of laying eggs fine, and bumblebee just incubates pupa and more easily obtains, and cost is also lower, passes through a large amount of repetition tests and draws.
Substituting host's chemistry stupor is to have narcotic cotton ball to be placed in the airtight glassware with dripping alternative host, substitute host's stupor in 5-10 minute, anaesthetic is that concentration is the medical ether of 3.6%-6.5%, adopt medical ether to anaesthetize to substituting the host, effect is better, and can not destroy the characteristic that substitutes the host.
Substituting host's chemistry stupor is to get swollen leg honeybee to become honeybee 1000-2000 head, be placed in the abrasive body and grind, getting the pure shrend of 10ml gets, filter, extract to quench to get to inject behind the liquid with small size syringe and substitute the host and make its stupor, adopt the venom of swollen leg honeybee that it is anaesthetized, anaesthetic effect does not influence the amount of breeding of swollen leg honeybee preferably simultaneously.
The present invention overcome substitute when existing swollen leg honeybee breeds the host obtain be subjected to time restriction, the source is limited can't satisfy the problem that goes up swollen leg honeybee wilderness demand of producing, induce behind host's induced spawning with substituting host's best cultivation by employing, the amount of breeding of swollen leg honeybee is increased substantially, reduced the influence that is subjected to cost and time restriction when alternative host chooses simultaneously.
Embodiment
The induction type leg honeybee mating system that swells, step is as follows: a, buy swollen leg honeybee kind honeybee low temperature and preserve; B, buy and induce host's low temperature to preserve; C, buy and substitute the host and carry out chemistry stupor back low temperature treatment, described alternative host is the first pupa of incubating of barley worm; D, the leg honeybee of will swelling are seeded in to induce and carry out induced spawning on the host; E, put into and cultivate after substituting the host; Transport behind f, the cryopreservation.
Buy to select for use when swollen leg honeybee kind honeybee low temperature is preserved and grow the apterergate healthy and strong, that fertility is strong, preserve swollen leg honeybee kind honeybee and should place stored refrigerated under 2-5 ℃ the temperature as kind of a honeybee.
Induce the host to select for use Cathay poplar longicorn pupa or honeybee just to incubate pupa: the insect gall that has Cathay poplar longicorn pupa that will buy is cut, and puts into stored refrigerated under 2-8 ℃ the temperature; The honey of buying back that has on the honeybee spleen that honeybee just incubates pupa is cleaned out, removed the honeybee frame, whole plate honeybee spleen is put into stored refrigerated under 2-5 ℃ the temperature.
The leg honeybee of will swelling is seeded in to induce and carries out induced spawning on the host, adopt following dual mode: first kind is that the host that induces that will refrigerate earlier takes out, put under 20-26 ℃ the environment improvements 4-8 hour, the swollen leg honeybee of refrigeration is taken out, put under 20-26 ℃ the environment improvements 1-2 hour, put the host that induces in vitro bottom through getting warm again after a cold spell, to insert test tube through the swollen leg honeybee that gets warm again after a cold spell, swollen leg honeybee is 20-40:1 with inducing host's inoculative proportion, after having connect kind the test tube mouth is clogged with cotton, the test tube bottom is put into does not outwardly have the test tube of top cover box, and the height of test tube box is lower than test tube, to expose the test tube bottom, the inner painted black of each test tube box is used for lucifuge, puts into 50-100 test tube in each test tube box, again the test tube box is kept flat and carries out induced spawning in the insulating box, with test tube bottom towards the place that light is arranged.Thermostat Temperature Control is at 24-30 ℃, and humidity is controlled at 50%-70%, induces 2-4 days;
Second kind is that the host that induces that will refrigerate earlier takes out, put under 20-26 ℃ the environment improvements 4-8 hour, the swollen leg honeybee of refrigeration is taken out, put under 20-26 ℃ the environment improvements 1-2 hour, the host that induces through getting warm again after a cold spell is put into the cultivation box, one deck toilet paper or fine sand cloth are put in the bottom of cultivating the box internal layer, put one deck then uniformly and induce the host, insert swollen leg honeybee kind honeybee again, swollen leg honeybee is 20-40:1 with inducing host's inoculative proportion, will cultivate the box upper end cover again and cover, to cultivate box puts into insulating box and carries out induced spawning, the constant humidity cabinet temperature is controlled at 24-30 ℃, and humidity is controlled at 50%-70%, induces 2-4 days;
Cultivate box by two ectonexine box bodys and exist the gap to be nested together to form on every side, be installed with lead screw between the two box body bottom faces, be screwed with the packing ring of being close to the internal box lower surface on the lead screw, washer height is greater than 20 millimeters, scribble Fan Silin on the packing ring, prevent that swollen leg honeybee from escaping, outside box body upper end cover has the upper end cover that husky net is housed, be used to observe swollen leg honeybee and breed situation and ventilation, husky mesh is less than the size of swollen leg honeybee individuality.
Cultivate after putting into alternative host, after treating that swollen leg honeybee lays eggs, put into to substitute before the host earlier and will and just incubate pupa through the chemistry stupor and be fixed on and breed on the fixator through the barley worm after the low temperature treatment, the barley worm just incubates and has reduced the twisting that the barley worm just incubates the pupa polypide after pupa is fixed, reduce its mobility, the barley worm is just incubated the pupa afterbody to be inserted in the pore of breeding fixator, head is contained in the annulus, with cotton ball jam-pack annulus, polypide is fixed, after improvements 1-2 hour, put in vitro or cultivate box and cultivate; Test tube box or cultivation box are put into climatic chamber, and temperature is controlled at 24-30 ℃, and humidity is controlled at 50%-70%.Optimum temperature is 26 ℃, can finish a life from generation to generation in about 6-20 days.The average weight that barley worm just incubates pupa is about 20 times of Cathay poplar longicorn pupa average weight, and its bee yield can reach more than 5 times of Cathay poplar longicorn pupa, can breed out swollen leg honeybee of 300-500 chieftain's generation, and numerous honeybee efficient is improved greatly.
Transport behind the cryopreservation, treated behind the swollen leg bee eclosion of filial generation that swollen leg honeybee became the abundant post-coitum of honeybee 3-5 days, with the test tube box or cultivate box and put into 2-5 ℃ environment refrigeration down; During pest control, test tube box or cultivation box are placed on the polarstream, temperature remains on 2-10 ℃ and transports to the destination.Before discharging the refrigerator car temperature is heightened 15-20 ℃, promote swollen leg honeybee steadily to revive 1-2 hour, guarantee swollen leg honeybee survival rate and good activity, just can discharge pest control then.
Inducing the host is that bumblebee just incubates pupa, the bumblebee of buying back is just incubated pupa put into the plastic casing that top cover has aperture, the plastic casing bottom is put one deck toilet paper or crocus cloth earlier, evenly put one deck bumblebee more thereon and just incubate pupa, lid layer toilet paper or crocus cloth again, put one deck bumblebee again and just incubate pupa, total number of plies surpasses the 5-6 layer, then plastic casing is put into stored refrigerated under 2-5 ℃ the temperature.
Substituting host's chemistry stupor is to have narcotic cotton ball to be placed in the airtight glassware with dripping alternative host, substitutes host's stupor in 5-10 minute, and anaesthetic is that concentration is the medical ether of 3.6%-6.5%.
Substituting host's chemistry stupor is to get swollen leg honeybee to become honeybee 1000-2000 head, is placed in the abrasive body and grinds, and gets the pure shrend of 5-15ml and gets, filters, and extracts to quench to get with small size syringe and injects alternative host behind the liquid and make its stupor.
Claims (4)
1. the swollen leg honeybee mating system of an induction type, it is characterized in that: step is as follows: a, buy swollen leg honeybee kind honeybee low temperature and preserve; B, buy and induce host's low temperature to preserve; C, buy and substitute the host and carry out chemistry stupor back low temperature treatment, described alternative host is the first pupa of incubating of barley worm; D, the leg honeybee of will swelling are seeded in to induce and carry out induced spawning on the host; E, put into and cultivate after substituting the host; Transport behind f, the cryopreservation.
2. the induction type according to claim 1 leg honeybee mating system that swells, it is characterized in that: inducing the host is that bumblebee just incubates pupa.
3. according to claim 1 and the swollen leg honeybee mating system of 2 described induction types, it is characterized in that: substituting host's chemistry stupor is to have narcotic cotton ball to be placed in the airtight glassware with dripping alternative host, substitute host's stupor in 5-10 minute, anaesthetic is that concentration is the medical ether of 3.6%-6.5%.
4. the induction type according to claim 1 and 2 leg honeybee mating system that swells, it is characterized in that: substituting host's chemistry stupor is to get swollen leg honeybee to become honeybee 1000-2000 head, be placed in the abrasive body and grind, get the pure shrend of 5-15ml and get, filter, extract to quench to get to inject behind the liquid with small size syringe and substitute the host and make its stupor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110102239 CN102144621A (en) | 2011-04-22 | 2011-04-22 | Induced scleroderma guani breeding method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110102239 CN102144621A (en) | 2011-04-22 | 2011-04-22 | Induced scleroderma guani breeding method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102144621A true CN102144621A (en) | 2011-08-10 |
Family
ID=44419259
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110102239 Pending CN102144621A (en) | 2011-04-22 | 2011-04-22 | Induced scleroderma guani breeding method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102144621A (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102524184A (en) * | 2011-12-22 | 2012-07-04 | 段东红 | Preparation method of composite natural enemy spray |
| CN103004704A (en) * | 2013-01-05 | 2013-04-03 | 北京市西山试验林场 | Method for breeding Scleroderma guani Xiao et Wu by using barley pests as intermediate hosts |
| CN104920308A (en) * | 2015-06-25 | 2015-09-23 | 中国林业科学研究院森林生态环境与保护研究所 | Method for artificial breeding of larch sclerodermus sp. and application of larch sclerodermus sp. for prevention of massicus raddei |
| CN104996362A (en) * | 2015-06-25 | 2015-10-28 | 中国林业科学研究院森林生态环境与保护研究所 | Method for artificially breeding sclerodermus pupariae yang et yao and applying sclerodermus pupariae yang et yao to control trunk-boring insects |
| CN104996236A (en) * | 2015-07-24 | 2015-10-28 | 贵州师范大学 | Method for preventing and treating dendroctonus valens LeConte by using scleroderma guani Xiao et Wus |
| CN106417190A (en) * | 2016-09-28 | 2017-02-22 | 天津拓华生物科技有限责任公司 | Method for pretreating sleroderma-guani alternate hosts |
| CN111149772A (en) * | 2020-01-14 | 2020-05-15 | 南京农业大学 | Method for extracting parasitic bee venom by using artificial host |
| CN111990344A (en) * | 2020-09-18 | 2020-11-27 | 贾自峰 | Manual bee receiving tube for scleroderma guani |
| CN112167174A (en) * | 2020-10-07 | 2021-01-05 | 三江县连兴蛇业有限公司 | Rapid wasp breeding method |
| CN115024283A (en) * | 2022-06-29 | 2022-09-09 | 贵州师范大学 | Method for preventing and treating dendrobe cyrtotrachelossis larva and pupa by using scleroderma guani and application of method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1465239A (en) * | 2002-07-31 | 2004-01-07 | 北京市植物园 | Scaled breeding for semanotus bifasciatus and application in biological preventing and controlling pest-insect |
| CN1669415A (en) * | 2005-04-05 | 2005-09-21 | 周祖基 | Scleroderma sichuanensis Xiao artificial reproduction method |
-
2011
- 2011-04-22 CN CN 201110102239 patent/CN102144621A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1465239A (en) * | 2002-07-31 | 2004-01-07 | 北京市植物园 | Scaled breeding for semanotus bifasciatus and application in biological preventing and controlling pest-insect |
| CN1669415A (en) * | 2005-04-05 | 2005-09-21 | 周祖基 | Scleroderma sichuanensis Xiao artificial reproduction method |
Non-Patent Citations (2)
| Title |
|---|
| 《中国生物防治》 20080831 姚万军等 人工繁殖管氏肿腿蜂的替代寄主研究 220-226 1-4 第24卷, 第3期 2 * |
| 《昆虫学报》 20050630 黄琼等 繁育川硬皮肿腿蜂替代寄主的筛选 375-379 1-4 第48卷, 第3期 2 * |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102524184A (en) * | 2011-12-22 | 2012-07-04 | 段东红 | Preparation method of composite natural enemy spray |
| CN103004704A (en) * | 2013-01-05 | 2013-04-03 | 北京市西山试验林场 | Method for breeding Scleroderma guani Xiao et Wu by using barley pests as intermediate hosts |
| CN104920308A (en) * | 2015-06-25 | 2015-09-23 | 中国林业科学研究院森林生态环境与保护研究所 | Method for artificial breeding of larch sclerodermus sp. and application of larch sclerodermus sp. for prevention of massicus raddei |
| CN104996362A (en) * | 2015-06-25 | 2015-10-28 | 中国林业科学研究院森林生态环境与保护研究所 | Method for artificially breeding sclerodermus pupariae yang et yao and applying sclerodermus pupariae yang et yao to control trunk-boring insects |
| CN104996362B (en) * | 2015-06-25 | 2018-01-26 | 中国林业科学研究院森林生态环境与保护研究所 | A kind of swollen leg honeybee of artificial breeding Chinese wax Ji fourth and the method applied to preventing and treating trunk borer |
| CN104996236A (en) * | 2015-07-24 | 2015-10-28 | 贵州师范大学 | Method for preventing and treating dendroctonus valens LeConte by using scleroderma guani Xiao et Wus |
| CN106417190A (en) * | 2016-09-28 | 2017-02-22 | 天津拓华生物科技有限责任公司 | Method for pretreating sleroderma-guani alternate hosts |
| CN111149772A (en) * | 2020-01-14 | 2020-05-15 | 南京农业大学 | Method for extracting parasitic bee venom by using artificial host |
| CN111149772B (en) * | 2020-01-14 | 2020-11-24 | 南京农业大学 | Method for extracting parasitic bee venom by using artificial host |
| CN111990344A (en) * | 2020-09-18 | 2020-11-27 | 贾自峰 | Manual bee receiving tube for scleroderma guani |
| CN112167174A (en) * | 2020-10-07 | 2021-01-05 | 三江县连兴蛇业有限公司 | Rapid wasp breeding method |
| CN115024283A (en) * | 2022-06-29 | 2022-09-09 | 贵州师范大学 | Method for preventing and treating dendrobe cyrtotrachelossis larva and pupa by using scleroderma guani and application of method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102144621A (en) | Induced scleroderma guani breeding method | |
| CN101785445B (en) | Scleroderma guani breeding method | |
| CN108056070B (en) | Method for breeding trichogramma by using snowflake moth | |
| CN105766803B (en) | Egg of oak silkworm mixes breeding trichogramma dendrolimi and Trichogramma Ostriniae method for host | |
| CN103004704A (en) | Method for breeding Scleroderma guani Xiao et Wu by using barley pests as intermediate hosts | |
| CN102144620B (en) | Method for cultivating earthworms for experiment | |
| CN102550489B (en) | Artificial propagation method for Ooencyrtus kuvanae | |
| Mies et al. | Giant clam aquaculture: a review on induced spawning and larval rearing | |
| CN107439491A (en) | Strengthen the pupa parasitic wasp artificial breeding production method of drosophila class biological control effect | |
| CN105028333A (en) | Method for artificially and largely raising Dioryctria pryeri indoors | |
| CN102630534B (en) | Method for controlling pine wood nematode diseases by utilizing body surface bacteria of American pine wood nematodes | |
| CN103975903B (en) | The raising method of a kind of butterfly section hairstreak | |
| Cock | The use of parasitoids for augmentative biological control of pests in the People's Republic of China. | |
| CN109042542B (en) | Method for breeding propylaea japonica by using semi-artificial feed | |
| CN102550490B (en) | Method of raising ectropis oblique warren through sambucus chinensis and Ectropis grisescens nucleopolyhedrovirus (EcobNPV) propagation method utilizing same | |
| Moghadasi et al. | Prey stages preference of different stages of Typhlodromus bagdasarjani (Acari: Phytoseiidae) to Tetranychus urticae (Acari: Tetranychidae) on rose | |
| Dindo et al. | Production of dipteran parasitoids | |
| CN103704178B (en) | A kind of regulate and control full skirt chase after the method for posting fly diapause | |
| CN105532583B (en) | A kind of living body propagation method of entomopathogenic nematode | |
| CN114391515B (en) | Evaluation method for indoor control effect of Sciadopitys yunnanensis by using scleroderma guani and application of evaluation method | |
| US20220272984A1 (en) | Feed Stuff for Beneficial Organisms that can be used in Integrated Pest Management | |
| Shields et al. | Multi-year biological control of black vine weevil, Otiorhynchus sulcatus, with persistent entomopathogenic nematodes | |
| CN104920066B (en) | The method for improving host of Cordyceps sinensis infection rate | |
| CN106259085A (en) | A kind of Trionyx sinensis (Wiegmann) propagation method | |
| Whitman | Laboratory biology of Taeniopoda eques (Orthoptera: Acrididae) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20110810 |