CN107439491A - Strengthen the pupa parasitic wasp artificial breeding production method of drosophila class biological control effect - Google Patents
Strengthen the pupa parasitic wasp artificial breeding production method of drosophila class biological control effect Download PDFInfo
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Abstract
The present invention relates to agricultural pests field of biological control, it is desirable to provide a kind of pupa parasitic wasp artificial breeding production method for strengthening drosophila class biological control effect.This method includes：The parasitic Drosophila melanogaster pupa of hair angle clavicorn serphid is collected, sprouts wings and the parasitism that continuous three generations after honeybee is used for extra large De Shi drosophilas pupa, collect honeybee kind；To extra large De Shi drosophilas inoculation reproduction, progress of then being pupated with the queen bee of parasitic wasp to extra large De Shi drosophilas is parasitic, culture, collects the parasitic wasp after sprouting wings and preservation is reserved seed for planting.The present invention a mao parasitic time for angle clavicorn serphid is controlled in seeded process produce, avoid because low parasitic rate caused by the parasitic time is too short or because excessively parasitism and caused by low bee hatching rate；More convenient reasonable inoculation and utilization in parasitic wasp is cultivated to hair angle clavicorn serphid；Large-scale continuous production can be carried out, improves advantage Tropical area and numerous bee technology, high-quality, efficient, high-volume breeding hair angle clavicorn serphid is realized, good basis has been established for the breeding utilization of hair angle clavicorn serphid.
Description
Technical field
The present invention relates to agricultural pests field of biological control, the pupa for more particularly to strengthening drosophila class biological control effect is parasitic
Honeybee artificial breeding production method, belong to the rearing method of advantage host and to strengthening drosophila class pest biological control effect
Pupa parasitic wasp artificial breeding production method.
Background technology
Drosophila feeding habits are extensive, and growing environment is special, like dark moist habitat, have to sour-sweet and corrupt smell bright
Aobvious taxis, can cause harm a variety of gourd, fruit and vegetables and the succulence organ of many plants, be important agricultural pests.When drosophila endangers farming
Exist during thing generally in the form of hybrid, including Drosophila melanogaster Drosophila melanogaster, her rice drosophila
D.immigrans, spotted wing drosophila D.suzukii etc..Parasitic wasp can control the population quantity of host, be important biological control
The factor.Therefore, Population Control is carried out to drosophila class pest using natural enemy insect more and more in integrated pest control management.
Hair angle clavicorn serphid Trichopria drosophilae Perkins, belong to Hymenoptera Diapriidae, are drosophilas
The main parasitic honeybee in pupa time, host range are wide.In laboratory conditions, its parasitic efficiency is higher than ring abdomen cynipidae Figitida's
Two kinds of main drosophila larvae parasitic wasps, but drosophila pupa time parasitic wasp is seldom directed in the research of drosophila-parasitic wasp system at present,
And hair angle clavicorn serphid is even more to be rarely reported before spotted wing drosophila turns into global insect.
Application number CN201610213523.3 Chinese patent discloses a kind of interior and breeds a mao method for angle clavicorn serphid,
But because host's spotted wing drosophila colony worm state development employed in embodiment differs, and artificial breeding complicated condition, it is unfavorable for
Standardization and extensive raising, therefore do not offered convenience to largely obtaining hair angle clavicorn serphid in actual production；Application number
A kind of method that fly pupa tiny golden wasp is cultivated using Hosts of CN201210448362.8 Introduction To Cn Patent, the embodiment
In come from advantage host parasitic wasp there is stronger control effect to drosophila, but the survival ability of fly pupa tiny golden wasp and parasitic imitate
Every biological characteristics such as rate is not as good as hair angle clavicorn serphid.
From the hair angle clavicorn serphid eclosion rate of Drosophila melanogaster pupa and parasitic efficiency high, long lifespan, fertility is strong, is for development
Prevent and treat the good source in terms of drosophila.But compared to Drosophila melanogaster, the extra large De Shi drosophilas D.hydei life-spans are longer, and reproductive capacity is stronger,
It is the advantage Hosts for breeding parasitic wasp and each stage of development individual is bigger.There has been no biological control hair angle hammer for the existing country
Angle serphid and the research report in terms of applying it to preventing and treating drosophila class pest, if can vast propagation hair angle clavicorn serphid, it is right
The effect for improving control of insect in actual production has important reference significance.
The content of the invention
The technical problem to be solved in the present invention is to overcome deficiency of the prior art, there is provided one kind strengthens drosophila class biology
The pupa parasitic wasp artificial breeding production method of prevention effect.
In order to solve the above technical problems, the solution of the present invention is：
A kind of pupa parasitic wasp artificial breeding production method for strengthening drosophila class biological control effect, including following steps are provided
Suddenly：
(1) collect and expand numerous honeybee kind
The parasitic Drosophila melanogaster pupa of hair angle clavicorn serphid is collected, it is permanent to be placed in the constant temperature that temperature is 25 ± 1 DEG C, RH is 50%
Developed in wet incubator；After the pupa being collected into is sprouted wings and honeybee, first it is fully mated, be then 1: 10 by queen bee and fly pupa
Ratio be placed in the blake bottle equipped with hair angle clavicorn serphid food carry out it is parasitic expand numerous, treat that the parasitic spawning of queen bee finishes and move queen bee
Go out；
By obtained hair angle clavicorn serphid offspring in the same way continuous three generations be used for extra large De Shi drosophilas pupa parasitism, make
Hair angle clavicorn serphid population from extra large De Shi drosophilas pupa is stabilized, and finally collects 50 or so bodies and grows at 4.5~4.9 millimeters
Drone and 200 or so bodies grow in the honeybee kind of 3.6~4.0 millimeters of queen bee as artificial propagation, it is 18 to raise in temperature
± 1 DEG C, the interior preservation of the constant temperature and humidity incubator that RH is 50%；
(2) inoculation reproduction of extra large De Shi drosophilas
Take sprout wings latter week left and right and the extra large De Shi drosophilas 25 that fully mate, the ratio of female drosophila and male drosophila is 20:
5；With CO2Anaesthetize and be put into the blake bottle equipped with extra large De Shi drosophilas food, cultivated in 25 DEG C of insulating box to spawning 100-
Drosophila is taken out after 120；Blake bottle is placed in the constant temperature and humidity incubator that temperature is 25 ± 1 DEG C, RH is 50%, observes and remember
Record starts the time of pupating, number of pupating and Growth of Drosophila Melanogaster situation；
(3) clavicorn serphid in hair angle is inoculated with
After extra large De Shi drosophilas pupate 2 days in step (2), chosen in the honeybee kind of the hair angle clavicorn serphid preserved from step (1)
The queen bee of 1/10 pupa number is put into the former cultivation bottle；Observation queen bee pupates to extra large De Shi drosophilas carries out parasitism, takes out and posts after 12h
Raw honeybee；
(4) culture of hair angle clavicorn serphid and collection
Trained the constant temperature and humidity that temperature is 25 ± 1 DEG C, RH is 50% is placed in by the extra large De Shi drosophilas pupa of parasitism in step (3)
Support in case, when parasitic wasp is developed to the 15th day in Host Pupae, parasitic wasp starts to sprout wings successively, collects the parasitism after sprouting wings
Honeybee；
(5) reserve seed for planting
The hair angle clavicorn serphid being collected into step (4) is put into the culture tube equipped with parasitic wasp food, culture tube is put
In preservation in the constant temperature and humidity incubator that temperature is 18 ± 1 DEG C, RH is 50%；100 or so, during for next breeding are preserved altogether
The inoculation of hair angle clavicorn serphid.
In the present invention, the collocation method of the parasitic wasp food is：Take 27g agar to add in 1000ml water, be sufficiently stirred
High-temp steam sterilizing is carried out after mixing；Then 33g brown sugar and the pure ciders of 330ml are added, is sufficiently stirred natural cooling after mixing
To 60 DEG C；100g methyl hydroxybenzoates are dissolved in 900ml 95% ethanol, then adds in the mixture cooled down, fully stirs
Mixing is mixed, 4 DEG C of refrigerations are standby.
In the present invention, the collocation method of the extra large De Shi drosophilas food is：Dry ferment 80g is taken to add molten in 300ml warm water
Solution；Weigh agar 10g, tryptone 20g, yeast extract 20g, sucrose 30g, glucose 60g to add in 700ml warm water, fill
Divide after stirring and evenly mixing and add the 300ml yeast solns dissolved, heat and stir；Thing to be mixed sequentially adds 0.5g when cooked
MgSO4·7H2O and CaCl2·2H2O, when 50~60 DEG C are naturally cooled to after boiling, add 6ml propionic acid, 10ml methyl hydroxybenzoates
Solution and 1g sodium benzoates simultaneously stir, and are sealed after cooling down 6~7h, it is standby to be put into 4 DEG C of refrigerator cold-storages；The methyl hydroxybenzoate
Solution is that 95% ethanol that 900ml is dissolved in 100g methyl hydroxybenzoates forms.
It is described to collect the parasitic Drosophila melanogaster pupa of hair angle clavicorn serphid in the present invention, operate in the steps below：Choosing
Select that insect is active and the season of rare extreme weather, be placed around drosophila parasitic wasp trap at the water source that vegetation is enriched, three
Reclaimed after week, collect the Drosophila melanogaster pupa that hair angle clavicorn serphid is parasitic in trap.
Inventive principle describes：
Hair angle clavicorn serphid parasitic scope is wide, not the obligatory parasitism honeybee of Drosophila melanogaster, while outdoor trapping honeybee kind,
It is also possible to capture other kinds of Drosophila simultaneously.Selection wherein individual is big, the wild-type Drosophila of high sensitivity, through morphology and
Molecular biology identification is extra large De Shi drosophilas, as host.It is if (beautiful with the conventional food formula of laboratory rearing Drosophila melanogaster
Ground rice-yeast-agar) the extra large De Shi drosophilas of raising, larva energy is low, and ability of creeping is weak, is often embedded in food bottom and pupates, this is not
Only it is unfavorable for the Reproductive development of host, and is made troubles for the parasitism of hair angle clavicorn serphid and emergence.
The present invention have adjusted the main nutrient composition ratio of former food preparation, that is, increase egg by constantly testing improvement
The intake of white matter and sugar, so as to not only extend life-span of host drosophila, shorten its life cycle, while spawning rate and pupate
Rate also significantly improves, and this provides basic technical support for the artificial biological control effect for strengthening hair angle clavicorn serphid.
Compared with the hair angle clavicorn serphid bred using Drosophila melanogaster fly pupa, the hair angle of extra large De Shi drosophilas fly pupa acquisition is used
The morphological feature of clavicorn serphid and in the control to Drosophila melanogaster, efficiency is more prominent, two kinds of propagation method comparing results
It is as follows：Compared with the hair angle clavicorn serphid bred by Drosophila melanogaster fly pupa of experimental determination, the hair angle that the present invention obtains is hammered into shape
Angle serphid has higher eclosion rate and produces female rate, and with individual life span is significantly longer, individual morphology is significantly larger, queen bee is fertile
Time significantly extends, stores up the features such as smart ability significantly increases, anti-adversity ability significantly improves；In addition, with using Drosophila melanogaster fly pupa
Compare, in parasitic Drosophila melanogaster fly pupa, parasitic rate and parasitic efficiency significantly increase the hair angle clavicorn serphid obtained with the present invention
Greatly.
Compared with prior art, the beneficial effects of the invention are as follows：
1st, the present invention cultivates more convenient reasonable inoculation and utilization in parasitic wasp to hair angle clavicorn serphid；
2nd, the present invention controls a mao parasitic time for angle clavicorn serphid in seeded process is produced, and avoids because of the parasitic time
Low parasitic rate caused by too short or because it is excessively parasitic and caused by low bee hatching rate；
3rd, the present invention is required the inoculative proportion of hair angle clavicorn serphid, host's drosophila pupa, by host when being inoculated with parasitic
Concentrate in blake bottle, a mao time for angle clavicorn serphid host-location is greatly shortened, so as to improve the parasitic rate of parasitic wasp；
4th, the present invention can carry out large-scale continuous production, improve advantage Tropical area and numerous bee technology, realize high-quality, high
Effect, high-volume breeding hair angle clavicorn serphid are the breeding of hair angle clavicorn serphid using having established good basis；
5th, breeding, storage and release of the present invention to other honeybees has reference role；
6th, the present invention improves the prevention effect to the berry class pest such as drosophila, can be more applied to Agricultural pest control field
Meet agricultural production needs in big degree.
Brief description of the drawings
Fig. 1 is the hair angle clavicorn serphid artificial progagation schematic flow sheet of the present invention.
Embodiment
The present invention is described in further detail with embodiment below in conjunction with the accompanying drawings：
As shown in figure 1, the inoculation expanding propagation method of hair angle clavicorn serphid comprises the steps：
(1) collect and expand numerous honeybee kind；
Step (1) specifically includes：
A, honeybee kind is collected：Selection insect enlivens and the season of rare extreme weather, is placed around at the water source that vegetation is enriched
Drosophila parasitic wasp trap (application reference number is CN201621171038.6 Chinese patent), reclaims after three weeks, collects quilt
The parasitic Drosophila melanogaster pupa of hair angle clavicorn serphid, is placed in the constant temperature and humidity incubator that temperature is 25 ± 1 DEG C, RH is 50% and (is purchased from
Hangzhou Lai Bo bio tech ltd) in develop.
The determination methods of the parasitic Drosophila melanogaster pupa of hair angle clavicorn serphid：The pupa time of normal Drosophila melanogaster is 4 days, so
Aftershaft dissolves drosophila；Drosophila melanogaster pupa after the clavicorn serphid parasitism of hair angle needs 15-20 talent to have honeybee therefrom to sprout wings, and
During young honeybee is developed in drosophila pupa, it can be observed have the gradual color burn blackening of polypide in translucent puparium.Treat hair
Angle clavicorn serphid is collected after sprouting wings out of Drosophila melanogaster pupa, and it is carried out by approach such as taxology and molecular biology
Identification, it is hair angle clavicorn serphid to confirm the parasitic wasp.The present invention hammers into shape in the numerous grade operation of the expansion after the collection step to hair angle
The collection of angle serphid directly expands first generation parasitic wasp derived above numerous without using field acquisition mode.
B, parasitic wasp food formulation (1L)：Weigh 27g agar to add in 1000ml water, high temperature is carried out after being sufficiently stirred mixing
Steam sterilizing；33g brown sugar is then added, the pure ciders of 330ml, is sufficiently stirred mixing；, will after food naturally cools to 60 DEG C
100g methyl hydroxybenzoates are dissolved in 900ml 95% ethanol, are fully dissolved and are added in the food cooled down, are sufficiently stirred mixed
It is even；It is standby to saturating benzene drosophila pipe and plastics raising bottle (buying in Zhong Tai experiment equipments factory of Haimen City), 4 DEG C of refrigerations to dispense food.
C, honeybee kind expands numerous：After the pupa being collected into step A is sprouted wings and honeybee, first it is fully mated, press queen bee and fly afterwards
Pupa number is 1:(drone is not put in the blake bottle that 10 ratio is placed in step B), and it is numerous to carry out parasitic expansion.Treat the parasitic production of queen bee
Ovum, which finishes, to be moved out.
D, honeybee kind is preferably stable with population：By the obtained hair angle clavicorn serphid offspring obtained in step C with same side
The continuous three generations of method is used for the parasitism of extra large De Shi drosophilas pupa, enables the hair angle clavicorn serphid population from extra large De Shi drosophilas pupa steady
It is fixed；Final choice body is grown grows the female peak at 3.6~4.0 millimeters as artificial propagation in 4.5~4.9 millimeters of drone and body
Honeybee kind, the hair angle clavicorn serphid Xiong Feng and the hair angle female peak of clavicorn serphid of 200 or so of collection 50 or so.
E, honeybee kind preserves：The hair angle clavicorn serphid that will sprout wings in step D, raises in step B parasitic wasp food, Yu Wen
Spend in 18 ± 1 DEG C, the laboratory constant temperature and humidity incubator (being purchased from the Si He bio tech ltd of Nanjing hundred) that RH is 50%
Preserve；
(2) inoculation reproduction of extra large De Shi drosophilas；
Step (2) specifically includes：
F, extra large De Shi drosophilas food formulation (1L)：Dry ferment 80g is weighed, is dissolved with 300ml warm water；Weigh agar 10g, pancreas
Peptone 20g, yeast extract 20g, sucrose 30g, glucose 60g is in 700ml warm water, after being sufficiently stirred mixing, adds
The 300ml yeast solns of dissolving, heat and stir energetically；0.5g is separately added into when above-mentioned 1000ml foods are substantially cooked
MgSO4·7H2O and CaCl2·2H2O, small fire is changed after boiling, then boil 1-2min, Guan Huo, treat that food naturally cools to 50~60 DEG C
When, add 6ml propionic acid, 10ml Metagins ester solution (100g methyl hydroxybenzoates are dissolved in 900ml 95% ethanol) and 1g benzene first
Sour sodium, after stirring, in culture vessel of the packing food in step C, sealed afterwards with gauze；Treat that food cools down 6~7h
Afterwards, sealed, stuck on a piece of adhesive tape with packaging bag, be put into 4 DEG C of refrigerator cold-storages, with take with.
G, host drosophila inoculation reproduction：Take sprout wings latter week left and right and 25 (the female drosophilas of extra large De Shi drosophilas fully to mate：
Male drosophila=20:5), with CO2The blake bottle dispensed in step F is anaesthetized and be put into, is taken after 2h of being laid eggs in 25 DEG C of insulating box
Go out, make its 100-120 head of laying eggs.
H, the development of fly pupa：Drosophila bottle in 10 bottles of step G is placed in the Intelligent culture case in step A, observes and remembers
Record starts the time of pupating, number of pupating and Growth of Drosophila Melanogaster situation；
(3) clavicorn serphid in hair angle is inoculated with；
Step (3) specifically includes：
After the extra large De Shi drosophilas in step H pupate 2 days, the queen bee of 1/10 pupa number is chosen in the honeybee kind preserved from step E, is put
Carried out in Intelligent culture case in step A parasitic (not putting drone), parasitic wasp is taken out after 12h.
When carrying out parasitic inoculation, it is ensured that hair angle clavicorn serphid has been subjected to abundant mating, and energy is strong；Hair angle clavicorn is thin
Honeybee was in for two age in days pupa time.
(4) culture of hair angle clavicorn serphid and collection；
Step (4) specifically includes：
It will be placed in step (3) by the drosophila pupa of parasitism in the Intelligent culture case in step (1) A, treat parasitic wasp in host
When being developed to the 15th day in pupa, parasitic wasp starts to sprout wings successively；The parasitic wasp of emergence is collected into the food training in step (1) B
Support in pipe, and in the climatic chamber being stored in step (1) E.
(5) reserve seed for planting；
Step (5) specifically includes：
Take about 100 conditions according to step (1) E of hair angle clavicorn serphid in step (4) to preserve honeybee kind, educated for next time
Hair angle clavicorn serphid inoculation during kind.
Technique effect is summarized：
Compared with the hair angle clavicorn serphid bred using Drosophila melanogaster fly pupa, using the hair of technical scheme acquisition
Angle clavicorn serphid is respectively 100% (N=10), 96.50% (N to the parasitic rate of Drosophila melanogaster fly pupa, bee hatching rate and the female rate of production
=10) and 84.97% (N=10), with the hair angle clavicorn serphid of Drosophila melanogaster fly pupa breeding to the parasitic rate of Drosophila melanogaster fly pupa,
Bee hatching rate and the female rate of production are respectively 99.50% (N=10), 85.93% (N=10) and 59.65% (N=10).Both are posting
There was no significant difference in raw rate, but in bee hatching rate (P<0.05) and female rate (P is produced<0.05) on, the hair angle clavicorn of the invention obtained
Serphid has significant advantage.
The hair angle clavicorn serphid individual that the present invention obtains is bigger, no matter male and female, its feeler length is (male：P<0.01；It is female：P<
0.01), body length is (male：P<0.01；It is female：P<0.01), wing length is (male：P<0.01；It is female：P<0.01) development is noticeably greater than from black abdomen
The hair clavicorn serphid individual of drosophila fly pupa.
The hair angle clavicorn serphid fecundity that the present invention obtains is stronger：The maximum fish brood amount of single head queen bee is 133.5 ± 8.0
Individual (N=20), make its single head parasitic in the case where only mating once, its egg-laying season is 23.3 ± 3.1 days (N=20), average
Life-span is 26.2 ± 3.3 days (N=20)；Using the hair angle clavicorn serphid maximum fish brood amount of Drosophila melanogaster fly pupa breeding as 105.0 ±
9.1 (N=20), egg-laying season are 10.3 ± 0.5 days (N=20), and average life span is 12.2 ± 0.8 days (N=20).Thus may be used
, the hair angle clavicorn serphid life-span that the present invention obtains significantly extends (P<0.01), the egg-laying season also significantly extends (P<0.01).This hair
The single head queen bee of the hair angle clavicorn serphid of bright acquisition after only mating once, can postpartum generation sum be 198.0 ± 18.5 (N
=20), wherein queen bee is 74.0 ± 3.3 (N=20)；With the single head queen bee of the hair angle clavicorn serphid of Drosophila melanogaster fly pupa breeding
In the same circumstances can postpartum generation sum be 119.0 ± 18.7 (N=20), wherein queen bee is 30.0 ± 7.8 (N=20).
It can thus be concluded that the hair angle smart ability of clavicorn serphid queen bee storage that the present invention obtains is stronger, offspring individuals number (P<0.05) with queen bee (P<
0.05) number is significantly more shared by.
The hair angle clavicorn serphid that the present invention obtains also has significant advantage in terms of anti-adversity ability：In 4 DEG C of low temperature and 37 DEG C
Can survive at least 25 days (N=100) and 16 days (N=100) respectively under high temperature, in the case of starvation, can at most survive about 15
My god (N=100)；And with the hair angle clavicorn serphid of Drosophila melanogaster fly pupa breeding in 4 DEG C of low temperature with that can be survived respectively under 37 DEG C of high temperature
At least 15 days (N=100) and 8 days (N=100), in the case of starvation, it can at most survive about 7 days (N=100).Thus may be used
, the hair angle clavicorn serphid that the present invention obtains is in low temperature (P<0.05), high temperature (P<And starvation (P 0.05)<0.05) adverse circumstance such as
Under survival rate be all remarkably higher than with Drosophila melanogaster fly pupa breed hair angle clavicorn serphid.
The hair angle clavicorn serphid from Drosophila melanogaster fly pupa is developed at 25 DEG C, and RH is under 50% laboratory condition, if nothing is posted
Raw behavior, survival 40 days or so is only capable of, and by the hair angle clavicorn serphid of the invention obtained under the same conditions, the 40th after its emergence
It parasitic rate, bee hatching rate and the female rate of production still may be up to 97.78 ± 3.14% (N=90), 96.50 ± 2.91% (N=90)
With 72.85 ± 4.58% (N=90).
In summary, the hair angle clavicorn serphid that the present invention is bred has stronger prevention effect to Drosophila melanogaster.
Finally it should be noted that listed above is only the specific embodiment for illustrating the present invention.Obviously, the present invention not
It is limited to above example, there can also be many variations.One of ordinary skill in the art can be straight from present disclosure
Export or all deformations associated are connect, are considered as protection scope of the present invention.
Claims (4)
1. a kind of pupa parasitic wasp artificial breeding production method for strengthening drosophila class biological control effect, it is characterised in that including under
State step：
(1) collect and expand numerous honeybee kind
The parasitic Drosophila melanogaster pupa of hair angle clavicorn serphid is collected, is placed in the constant temperature and humidity training that temperature is 25 ± 1 DEG C, RH is 50%
Support and developed in case；After the pupa being collected into is sprouted wings and honeybee, first it is set fully to mate, then in the ratio that queen bee and fly pupa are 1: 10
Be placed in the blake bottle equipped with hair angle clavicorn serphid food carry out it is parasitic expand numerous, treat that the parasitic spawning of queen bee finishes and remove queen bee；
By obtained hair angle clavicorn serphid offspring in the same way continuous three generations be used for extra large De Shi drosophilas pupa parasitism, make to come from
The hair angle clavicorn serphid population of extra large De Shi drosophilas pupa is stabilized；Finally 50 or so bodies of collection grow the hero at 4.5~4.9 millimeters
Honeybee and 200 or so bodies are grown in the honeybee kind of 3.6~4.0 millimeters of queen bee as artificial propagation, and it is 18 ± 1 to raise in temperature
DEG C, RH be 50% constant temperature and humidity incubator in preserve；
(2) inoculation reproduction of extra large De Shi drosophilas
Take 25 sprout wings latter week left and right and the extra large De Shi drosophilas that fully mate, the ratio of female drosophila and male drosophila is 20: 5；With
CO2Anaesthetize and be put into the blake bottle equipped with extra large De Shi drosophilas food, cultivated in 25 DEG C of insulating box to spawning 100-120 heads
After take out drosophila；Blake bottle is placed in the constant temperature and humidity incubator that temperature is 25 ± 1 DEG C, RH is 50%, observes and record out
Begin time, number of pupating and the Growth of Drosophila Melanogaster situation of pupating；
(3) clavicorn serphid in hair angle is inoculated with
After extra large De Shi drosophilas pupate 2 days in step (2), 1/10 is chosen in the honeybee kind of the hair angle clavicorn serphid preserved from step (1)
The queen bee of pupa number is put into the former cultivation bottle；Observation queen bee pupates to extra large De Shi drosophilas, and progress is parasitic, and taking-up is parasitic after 12h
Honeybee；
(4) culture of hair angle clavicorn serphid and collection
The constant temperature and humidity incubator that temperature is 25 ± 1 DEG C, RH is 50% will be placed in by the extra large De Shi drosophilas pupa of parasitism in step (3)
Interior, when parasitic wasp is developed to the 15th day in Host Pupae, parasitic wasp starts to sprout wings successively, and the hair angle clavicorn collected after sprouting wings is thin
Honeybee；
(5) reserve seed for planting
The hair angle clavicorn serphid being collected into step (4) is put into the culture tube equipped with parasitic wasp food, culture tube is placed in temperature
Spend to be preserved in 18 ± 1 DEG C, the constant temperature and humidity incubator that RH is 50%；100 or so are preserved altogether, hair during for next breeding
Angle clavicorn serphid inoculation.
2. according to the method for claim 1, it is characterised in that the collocation method of the parasitic wasp food is：Take 27g agar
Add in 1000ml water, high-temp steam sterilizing is carried out after being sufficiently stirred mixing；Then 33g brown sugar and the pure ciders of 330ml are added,
60 DEG C are naturally cooled to after being sufficiently stirred mixing；100g methyl hydroxybenzoates are dissolved in 900ml 95% ethanol, then added cold
In good mixture, mixing is sufficiently stirred, 4 DEG C of refrigerations are standby.
3. according to the method for claim 1, it is characterised in that the collocation method of the extra large De Shi drosophilas food is：Take dry
Yeast 80g, which is added in 300ml warm water, to be dissolved；Weigh agar 10g, tryptone 20g, yeast extract 20g, sucrose 30g, grape
Sugared 60g is added in 700ml warm water, is added the 300ml yeast solns dissolved after being sufficiently stirred mixing, is heated and stir；Wait to mix
Compound sequentially adds 0.5g MgSO when cooked4·7H2O and CaCl2·2H2O, when 50~60 DEG C are naturally cooled to after boiling, add
Enter 6ml propionic acid, 10ml Metagins ester solution and 1g sodium benzoates and stir, sealed after cooling down 6~7h, be put into 4 DEG C of ice
Case refrigeration is standby；The Metagin ester solution is that 95% ethanol that 900ml is dissolved in 100g methyl hydroxybenzoates forms.
4. according to the method for claim 1, it is characterised in that described to collect the parasitic Drosophila melanogaster of hair angle clavicorn serphid
Pupa, operate in the steps below：Selection insect enlivens and the season of rare extreme weather, is put near the water source that vegetation is enriched
Drosophila parasitic wasp trap is put, is reclaimed after three weeks, collects the Drosophila melanogaster pupa that hair angle clavicorn serphid is parasitic in trap.
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