CN102293182B - Orius sauteri raising method - Google Patents
Orius sauteri raising method Download PDFInfo
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- CN102293182B CN102293182B CN 201110200775 CN201110200775A CN102293182B CN 102293182 B CN102293182 B CN 102293182B CN 201110200775 CN201110200775 CN 201110200775 CN 201110200775 A CN201110200775 A CN 201110200775A CN 102293182 B CN102293182 B CN 102293182B
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Abstract
The invention relates to the field of plant protection and the insect raising technology, in particular to an orius sauteri raising method adopting drosophila carcinophila as feedstuff. The method changes the traditional method of adopting aphid or frankliniella occidentalis as feedstuff, the defect of the orius sauteri raising caused by the problems of the two kinds of feedstuff per se is changed, the characteristics of short growth period of the drosophila carcinophila and low production environment requirement are utilized, the feedstuff basis is provided for the large-scale raising of the orius sauteri, and in addition, the feeding effect is very excellent.
Description
Technical field
The invention belongs to plant protection field and cultured insect technology, relate to a kind of raising method of Orius.
Background technology
Orius belongs to Semiptera section, is a kind of fore wing tool key, and tool is simple eye, the small-sized Lu Sheng stinkbug class insect of digitus 3 joints, and its adult and nymph can prey on the software small worms such as aphid, thrips, small-sized lepidopterous larvae, and mite class and various worm's ovum.Because Orius is widely distributed, the characteristics such as strong adaptability, quantity is many, predatism is strong, be considered to a class predatory natural enemy insect than the good utilisation prospect is arranged, therefore, in recent years, for realizing the extensive raising of Orius, its biology, oviposition substrate, feed and raising instrument etc. a series of researchs have been done both at home and abroad, although obtained certain achievement, raised in addition larger gap apart from scale.
The breeding of Orius has two conditions essential, and one is feed, and live body feed preferably can be edible for them as the mini pest of aphid, this class of Tetranychus cinnabarinus; Another condition is oviposition substrate exactly: Orius is liked laying eggs the tender shoots of bean seedlings, so select oviposition substrate to select the kidney bean seedling that just grows tender shoots.Wherein be used for feeding the feed of Orius, general aphid, Tetranychus cinnabarinus or the Frankliniella occidentalis of adopting is as main feed now, but in the above-mentioned feed, the cultivation overlong time of Frankliniella occidentalis, the Frankliniella occidentalis that the whole time approximately need to could obtain as the Orius feed in about one month, and the condition of cultivating is higher, the rising that can bring cost; And when adopting aphid and Tetranychus cinnabarinus as feed, the cultivation time is also longer, and is general all more than 20 days, and the required condition of the cultivation of aphid is harsher, and sanitary condition is poor, and the as easy as rolling off a log pollution that causes feeding environment causes the death of aphid; And when adopting Tetranychus cinnabarinus, have too above-mentioned problem, and when adopting above-mentioned two kinds of feeds, the lethality of Orius is higher, can't satisfy the requirement of mass rearing.
Summary of the invention
Problems for existing Orius artificial feeding existence, in order better to develop the extensive raising of East Asia Orius, the inventor is through long-term experiment, a kind of method that adopts drosophila larvae to raise Orius as feed is provided, before changing, this method adopt aphid or Frankliniella occidentalis as the method for feed, changed owing to these two kinds of feed built-in problems and the drawback that raising brings to Orius, utilize the drosophila larvae growth cycle short, production environment requires low characteristics, for the extensive raising of Orius provides the basis on the feed, and the effect of feeding is splendid.
The raising method of Orius of the present invention mainly comprises the steps:
(1) cultivation of oviposition substrate is packed the Nutrition Soil for preparing in the pot for growing seedlings into, after watering, imbed the embryo of kidney bean seed in the Nutrition Soil downwards, 1/4 is exposed at outside the soil, watered one time water in per 24 hours, take out when treating kidney bean seedling plant height 5cm, root is put into box for feeding pests with the absorbent cotton moisturizing of soaking and is laid eggs for Orius.
(2) screening of worm kind will gather the Orius adult that comes in the insectary with bean aphid raise set up stablize population after, filter out 1000 pairs of Orius, each box for feeding pests is inserted female male imago 20-25 pair of firm emergence, allow and lay eggs after its natural mating, collect the Orius ovum and use the worm kind as producing, changed 1 bean aphid feed in per 24 hours and changed 1 oviposition substrate in per 12 hours, collect continuously 5 days ovum, the band ovum matrix of collection places under the 3-5 ℃ of low temperature and saves backup;
(3) raising of Orius will place the box of laying eggs with the oviposition substrate of Orius ovum, under 25 ± 1 ℃, RH=75 ± 5%, 16L:8D condition, cultivate, can hatch in ovum 5-6 days, transfer to nymph in the plastics box for feeding pests of side opening with dactylethrae every day.The corrugated paper fringe is placed in the box for feeding pests bottom, and fresh clean kidney bean seedling, drosophila larvae and feed box thereof are placed on (single head adult Orius day predation amount is about: 30 of 10 of 60 of Frankliniella occidentalis or drosophila larvaes or bean aphids) on the corrugation paper slip.In feeding process, the drosophila larvae feed that more renewed in 2-3 days enters the adult stage after Orius experience 4-5 the length of time (10-12 days) stand-by.
Nutrition Soil described in the step (1) is to be that 1: 1: 1 ratio mixes with black earth, vermiculite and peat composed of rotten mosses ash according to volume ratio.Because Orius is liked laying eggs the tender shoots of bean seedlings, so oviposition substrate is selected the kidney bean seedling that just grows tender shoots, in whole feeding process, the kidney bean seedling not only can be used as oviposition substrate, also can be used as the water source of raising Orius.
The Orius of original adoption picks up from the field in the step (2).
The box of laying eggs in the step (3) generally adopts the specification of 20cm*13cm*7cm, and the specification of box for feeding pests is 60cm*50cm*30cm, and the corrugated paper fringe in the box for feeding pests can separate nymph effectively, reduces internecine chance.In order to keep the vigor of drosophila larvae, need to place the drosophila larvae feed in the box for feeding pests, simultaneously creep into the problem that Orius in the feed be can't see drosophila larvae for fear of drosophila larvae, only spread very thin one deck feed in the drosophila larvae feed box, changed once in general 2-3 days.
When adopting drosophila larvae to raise Orius, adopt the Orius of the drosophila larvae raising 1-2 age in days of 1 age in days; Adopt the Orius of the drosophila larvae raising 3-5 age in days of 2-3 age in days.Why like this design mainly is to consider that the drosophila larvae growth rate is very fast, so that growth rate and predation that can the balance Orius, the size of drosophila larvae when just coming the regulation raising according to the age in days of Orius.In order to play better effect, general drosophila larvae carries out independent cultivation, and its cultural method is as follows:
(1) configuration of medium: by weight, main component comprises: 600 parts in water, sugared 20-25 part, agar 8-10 part, corn flour or oatmeal 40-50 part, preservative 0.5-1 part, dusty yeast 10-15 part;
Its preparation method is: level is divided into two parts, a agar and the sugar of adding, another part adds corn flour or oatmeal, respectively heating and constantly stirring, treat that agar and sugar dissolve in water and is solution than thickness, corn flour or oatmeal dissolve in water and evenly distribute in the pasty state, again with the two admixed together stirring, slowly are heated to and boil, and keep boiling state 5 minutes, the medium that is homogeneous, stopped heating, slowly cooling;
When treating that the medium temperature is down to 74-76 ℃, add the preservative of formula ratio, stir, and divide while hot the Cans that install to sterilization;
Medium cools off but before not solidifying, spreads one deck dusty yeast on medium, to absorb the moisture on top layer, uses the bottle cap with thin copper mesh to seal bottleneck, and the medium cooled and solidified can be stand-by.
Wherein, sugar can be selected soft white sugar or brown sugar, and preservative is a kind of in sorbic acid, propionic acid or the benzoic acid.
(2) raising of fruit bat: open medium bottleneck for subsequent use, clean a bottle inwall with 70% cotton ball soaked in alcohol, dry in the rear bottle and access 10-15 to kind of a fly, build bottle cap, be placed under 25 ± 1 ℃, RH=75 ± 5%, 16L:8D illumination condition and cultivated 24 hours, female fly post-coitum produces ovum in medium, will plant the fly taking-up after laying eggs and put into other blake bottle, hatches in the insectary approximately with the medium of ovum and can obtain larva in 24 hours;
Larva hatches rear raising 2-3 days, adding distil water to 2/3 place in the blake bottle, and larva climbs out of to enter in the water body and with screen filtration from medium isolates larva;
With the tweezers that go sharp passivation with the cotton ball soaked in alcohol sterilization after, drosophila larvae is taken out from feed, put into 0-4 ℃ of refrigerating box refrigeration 15-20 minute, put into the box for feeding pests of raising Orius after reducing the mobility of larva.
When treating that fruit bat grows into adult, support in the worm bottle and put into fresh culture, lay eggs for it, the medium with ovum is collected, place 4-8 ℃ of refrigerator conservation; The pupa of fruit bat also can be used for conservation, and drosophila larvae experience just enters pupa time after three length of times, with pupa take out place 4-8 ℃ stand-by, fruit bat pupa time is four days under 25 ± 1 ℃ condition, can obtain rapidly adult.
The present invention adopts drosophila larvae to feed Orius as feed, has following beneficial effect:
1, because drosophila larvae just can be ripe about 1 week, than existing Orius animal feed (bean aphid, Frankliniella occidentalis), the production cycle shortens significantly;
2, drosophila larvae contains the nutrient components such as rich in protein, fatty acid, amino acid, chitin, vitamin, mineral element, macroelement and micronutrient levels are abundant, detected more than 17 kinds, fat content is about 12% in its dry powder, unsaturated fatty acid accounts for 68%, and essential fatty acid accounts for 36%, and nutrient component is comprehensive, contain Orius needed each seed amino acid that grows, can satisfy preferably the nutritional need that Orius grows and breeds;
3, fruit bat secretes the antibacterial substances such as a large amount of antibacterial peptides, can effectively prevent feed mold;
4, the production environment of fruit bat require low, production cost is low, breeding technology is ripe, growing is not subjected to seasonal effect, non-vulnerable to pollution, can be for a long time provides feed for the raising of Orius.
In sum, before changing, technical scheme of the present invention adopt aphid or Frankliniella occidentalis as the method for feed, changed owing to these two kinds of feed built-in problems and the drawback that raising brings to Orius, utilize the drosophila larvae growth cycle short, production environment requires low characteristics, for the extensive raising of Orius provides the basis on the feed, and the effect of feeding is splendid.
Embodiment
Embodiment 1
Raise the Orius adult with drosophila larvae, the cultural method of drosophila larvae is:
(1) configuration of medium: by weight, main component comprises: 600 parts in water, 22 parts in brown sugar, 10 parts in agar, 45 parts of oatmeals, 1 part of sorbic acid, 15 parts of dusty yeasts;
Its preparation method is: level is divided into two parts, a agar and the brown sugar of adding, another part adds oatmeal, respectively heating and constantly stirring, treat that agar and brown sugar dissolve in water and is solution than thickness, oatmeal dissolves in water and evenly distributes in the pasty state, again with the two admixed together stirring, slowly is heated to and boils, and keep boiling state 5 minutes, the medium that is homogeneous, stopped heating, slowly cooling;
When treating that the medium temperature is down to 74 ℃, add the sorbic acid of formula ratio, stir, and divide while hot the Cans that install to sterilization;
Medium cools off but before not solidifying, spreads one deck dusty yeast on medium, to absorb the moisture on top layer, uses the bottle cap with thin copper mesh to seal bottleneck, and the medium cooled and solidified can be stand-by;
(2) raising of fruit bat: open medium bottleneck for subsequent use, clean a bottle inwall with 70% cotton ball soaked in alcohol, dry 12 pairs of kind flies of access in the rear bottle, build bottle cap, be placed under 25 ± 1 ℃, RH=75 ± 5%, 16L:8D illumination condition and cultivated 24 hours, female fly post-coitum produces ovum in medium, will plant the fly taking-up after laying eggs and put into other blake bottle, hatches in the insectary approximately with the medium of ovum and can obtain larva in 24 hours;
Larva hatches rear raising 2 days, adding distil water to 2/3 place in the blake bottle, and larva climbs out of to enter in the water body and with screen filtration from medium isolates larva;
With the tweezers that go sharp passivation with the cotton ball soaked in alcohol sterilization after, drosophila larvae is taken out from feed, put into 2 ℃ of refrigerating boxes refrigerations 17 minutes, put into the box for feeding pests of raising Orius after reducing the mobility of larva.
The drosophila larvae of above-mentioned cultivation is used for the raising of Orius, mainly comprises the steps:
(1) cultivation of oviposition substrate is with 1: 1: 1 by volume ratio mixing of black earth, vermiculite and turfy soil, pack in the pot for growing seedlings, after watering, imbed the embryo of kidney bean seed in the Nutrition Soil downwards, 1/4 is exposed at outside the soil, watered once in per 24 hours, and took out when treating kidney bean seedling plant height 5cm, root is put into box for feeding pests with the absorbent cotton moisturizing of soaking and is laid eggs for Orius;
(2) screening of worm kind will gather the Orius adult that comes in the insectary with bean aphid raise set up stablize population after, filter out 1000 pairs of Orius, each box for feeding pests is inserted 20 pairs of the female male imagos of firm emergence, allow and lay eggs after its natural mating, collect the Orius ovum and use the worm kind as producing, changed 1 bean aphid feed in per 24 hours and changed 1 oviposition substrate in per 12 hours, collect continuously 5 days ovum, the band ovum matrix of collection places under 4 ℃ of low temperature and saves backup;
(3) raising of Orius will place the box of laying eggs with the oviposition substrate of Orius ovum, under 25 ± 1 ℃, RH=75 ± 5%, 16L:8D condition, cultivate, ovum can be hatched in 5 days, transfer to nymph in the plastics box for feeding pests of side opening every day with dactylethrae, the corrugated paper fringe is placed in the box for feeding pests bottom, and fresh clean kidney bean seedling, drosophila larvae and feed box thereof are placed on (single head adult Orius day predation amount is about: 30 of 10 of 60 of Frankliniella occidentalis or drosophila larvaes or bean aphids) on the corrugation paper slip.The drosophila larvae feed that more renewed in per 2 days enters the adult stage after Orius experiences 4 length of times stand-by.
Embodiment 2
Raise the Orius adult with drosophila larvae, the cultural method of drosophila larvae is:
(1) configuration of medium: by weight, main component comprises: 600 parts in water, 20 parts of soft white sugars, 8 parts in agar, 40 parts of corn flour, 0.7 part in benzoic acid, 10 parts of dusty yeasts;
Its preparation method is: level is divided into two parts, a agar and the soft white sugar of adding, another part adds corn flour, respectively heating and constantly stirring, treat that agar and soft white sugar dissolve in water and be solution than thickness, corn flour dissolves in water and evenly distributes in the pasty state, again with the two admixed together stirring, slowly is heated to and boils, and keep boiling state 5 minutes, the medium that is homogeneous, stopped heating, slowly cooling;
When treating that the medium temperature is down to 76 ℃, add the benzoic acid of formula ratio, stir, and divide while hot the Cans that install to sterilization;
Medium cools off but before not solidifying, spreads one deck dusty yeast on medium, to absorb the moisture on top layer, uses the bottle cap with thin copper mesh to seal bottleneck, and the medium cooled and solidified can be stand-by;
(2) raising of fruit bat: open medium bottleneck for subsequent use, clean a bottle inwall with 70% cotton ball soaked in alcohol, dry 15 pairs of kind flies of access in the rear bottle, build bottle cap, be placed under 25 ± 1 ℃, RH=75 ± 5%, 16L:8D illumination condition and cultivated 24 hours, female fly post-coitum produces ovum in medium, will plant the fly taking-up after laying eggs and put into other blake bottle, hatches in the insectary approximately with the medium of ovum and can obtain larva in 24 hours;
Larva hatches rear raising 3 days, adding distil water to 2/3 place in the blake bottle, and larva climbs out of to enter in the water body and with screen filtration from medium isolates larva;
With the tweezers that go sharp passivation with the cotton ball soaked in alcohol sterilization after, drosophila larvae is taken out from feed, put into 4 ℃ of refrigerating boxes refrigerations 20 minutes, put into the box for feeding pests of raising Orius after reducing the mobility of larva.
The drosophila larvae of above-mentioned cultivation is used for the raising of Orius, mainly comprises the steps:
(1) cultivation of oviposition substrate with black earth, vermiculite and turfy soil in 1: 1: 1 ratio mixing, pack in the pot for growing seedlings, after watering, imbed the embryo of kidney bean seed in the Nutrition Soil downwards, 1/4 is exposed at outside the soil, watered water one time in per 24 hours, and took out when treating kidney bean seedling plant height 5cm, root is put into box for feeding pests with the absorbent cotton moisturizing of soaking and is laid eggs for Orius;
(2) screening of worm kind will gather the Orius adult that comes in the insectary with bean aphid raise set up stablize population after, filter out 1000 pairs of Orius, each box for feeding pests is inserted 25 pairs of the female male imagos of firm emergence, allow and lay eggs after its natural mating, collect the Orius ovum and use the worm kind as producing, changed 1 bean aphid feed in per 24 hours and changed 1 oviposition substrate in per 12 hours, collect continuously 5 days ovum, the band ovum matrix of collection places under 3 ℃ of low temperature and saves backup;
(3) raising of Orius will place the box of laying eggs with the oviposition substrate of Orius ovum, at 25 ± 1 ℃, RH=75 ± 5%, cultivate under the 16L:8D condition, ovum can be hatched in 6 days, transfer to nymph in the plastics box for feeding pests of side opening every day with dactylethrae, the corrugated paper fringe is placed in the box for feeding pests bottom, with fresh clean kidney bean seedling, drosophila larvae and feed box be placed on (single head adult Orius day predation amount is about: 30 of 10 of 60 of Frankliniella occidentalis or drosophila larvaes or bean aphids) on the corrugation paper slip, the drosophila larvae feed that more renewed in per 3 days enters the adult stage after Orius experiences 5 length of times stand-by.
Embodiment 3
Raise the Orius adult with drosophila larvae, the cultural method of drosophila larvae is:
(1) configuration of medium: by weight, main component comprises: 600 parts in water, 25 parts in brown sugar, 9 parts in agar, 50 parts of oatmeals, 0.5 part of propionic acid, 12 parts of dusty yeasts;
Its preparation method is: level is divided into two parts, a agar and the brown sugar of adding, another part adds oatmeal, respectively heating and constantly stirring, treat that agar and brown sugar dissolve in water and is solution than thickness, oatmeal dissolves in water and evenly distributes in the pasty state, again with the two admixed together stirring, slowly is heated to and boils, and keep boiling state 5 minutes, the medium that is homogeneous, stopped heating, slowly cooling;
When treating that the medium temperature is down to 75 ℃, add the propionic acid of formula ratio, stir, and divide while hot the Cans that install to sterilization;
Medium cools off but before not solidifying, spreads one deck dusty yeast on medium, to absorb the moisture on top layer, uses the bottle cap with thin copper mesh to seal bottleneck, and the medium cooled and solidified can be stand-by;
(2) raising of fruit bat: open medium bottleneck for subsequent use, clean a bottle inwall with 70% cotton ball soaked in alcohol, dry 10 pairs of kind flies of access in the rear bottle, build bottle cap, be placed under 25 ± 1 ℃, RH=75 ± 5%, 16L:8D illumination condition and cultivated 24 hours, female fly post-coitum produces ovum in medium, will plant the fly taking-up after laying eggs and put into other blake bottle, hatches in the insectary approximately with the medium of ovum and can obtain larva in 24 hours;
Larva hatches rear raising 2 days, adding distil water to 2/3 place in the blake bottle, and larva climbs out of to enter in the water body and with screen filtration from medium isolates larva;
With the tweezers that go sharp passivation with the cotton ball soaked in alcohol sterilization after, drosophila larvae is taken out from feed, put into 0 ℃ of refrigerating box refrigeration 15 minutes, put into the box for feeding pests of raising Orius after reducing the mobility of larva.
The drosophila larvae of above-mentioned cultivation is used for the raising of Orius, mainly comprises the steps:
(1) cultivation of oviposition substrate with black earth, vermiculite and turfy soil in 1: 1: 1 ratio mixing, pack in the pot for growing seedlings, after watering, imbed the embryo of kidney bean seed in the Nutrition Soil downwards, 1/4 is exposed at outside the soil, watered water one time in per 24 hours, and took out when treating kidney bean seedling plant height 5cm, root is put into box for feeding pests with the absorbent cotton moisturizing of soaking and is laid eggs for Orius;
(2) screening of worm kind will gather the Orius adult that comes in the insectary with bean aphid raise set up stablize population after, filter out 1000 pairs of Orius, each box for feeding pests is inserted 22 pairs of the female male imagos of firm emergence, allow and lay eggs after its natural mating, collect the Orius ovum and use the worm kind as producing, changed 1 bean aphid feed in per 24 hours and changed 1 oviposition substrate in per 12 hours, collect continuously 5 days ovum, the band ovum matrix of collection places under 5 ℃ of low temperature and saves backup;
(3) raising of Orius will place the box of laying eggs with the oviposition substrate of Orius ovum, under 25 ± 1 ℃, RH=75 ± 5%, 16L:8D condition, cultivate, ovum can be hatched in 5 days, transfer to nymph in the plastics box for feeding pests of side opening every day with dactylethrae, the corrugated paper fringe is placed in box for feeding pests bottom, with fresh clean kidney bean seedling, drosophila larvae and feed box be placed on (single head adult Orius day predation amount is about: 30 of 10 of 60 of Frankliniella occidentalis or drosophila larvaes or bean aphids) on the corrugation paper slip.The drosophila larvae feed that more renewed in per 2 days enters the adult stage after Orius experiences 5 length of times stand-by.
Comparative example 1
Raise the Orius adult with bean aphid, bean aphid adopts prior art to cultivate, and approximately can supply in 4-5 days to raise general 8-10 days generation.Raising step according to the Orius described in the embodiment 1 is fed.
Comparative example 2
With Frankliniella occidentalis raising Orius adult, Frankliniella occidentalis adopts prior art to cultivate, and approximately can for raising, generally breed a generation in 25-30 days in 5-7 days.Raising step according to the Orius described in the embodiment 1 is fed.
Embodiment 1 is compared with comparative example 1 and 2, finds after two generations that fruit bat and Frankliniella occidentalis breeding grub feeding effect are best, bean aphid during as feed effect relatively poor.The Orius nymph lethality that drosophila larvae is raised is low, 10%-15% only, and it is long for about 12.21d that nymphal development is gone through the phase, can be the enough nutriments of adult stage accumulation, prolonged life-span and the egg-laying period of adult.The adult mean lifetime is about 19d, the longlyest reaches 26 days.The first generation Adult worms producting eggs phase, egg production peak time was at the 2nd to 6 day about 14 days, and single female average egg laying amount is 98.The second generation egg-laying period is elongated, and about 17 days, the egg laying amount of every day is average, and between 5 to 9, but single female egg laying amount does not reduce.
The Orius nymph lethality that Frankliniella occidentalis is raised is 10%-15%, and it is 12.19d that nymphal development is gone through the phase.The adult mean lifetime is about 19d, the longlyest reaches 24 days, and egg production peak time concentrates on the 2nd to 6 day, and single female average egg laying amount is 101, about average egg-laying period 14d.
The Orius nymphal development that bean aphid is raised is gone through shorter only 11.72d of phase, lethality about 20%, and single female egg laying amount is about 66.About average egg-laying period 11d, mean lifetime is about 14d.
Frankliniella occidentalis is that present feeding effect is best, but operating difficulties, cost compare is high, and the feeding effect of drosophila larvae of the present invention and Frankliniella occidentalis is suitable, and than the technology that Frankliniella occidentalis is fed, reduced the environmental pollution and the production cost that cause when the live body feed is raised, simple to operate, production cycle shortens significantly, is enough to a large amount of promotion and application.
Claims (6)
1. the raising method of an Orius is characterized in that: adopt drosophila larvae to raise Orius as feed, it mainly comprises the steps:
(1) cultivation of oviposition substrate
The Nutrition Soil for preparing is packed in the pot for growing seedlings, after watering, imbed the embryo of kidney bean seed in the Nutrition Soil downwards, 1/4 is exposed at outside the soil, watered water one time in per 24 hours, and took out when treating kidney bean seedling plant height 5cm, root is put into box for feeding pests with the absorbent cotton moisturizing of soaking and is laid eggs for Orius;
(2) screening of worm kind
With the Orius adult that gathers in the insectary with bean aphid raise set up stablize population after, filter out 1000 pairs of Orius, each box for feeding pests is inserted female male imago 20-25 pair of firm emergence, allow and lay eggs after its natural mating, collect the Orius ovum and use the worm kind as producing, changed 1 bean aphid feed in per 24 hours and changed 1 oviposition substrate in per 12 hours, collect continuously 5 days ovum, the band ovum matrix of collection places under the 3-5 ℃ of low temperature and saves backup;
(3) raising of Orius
To place the box of laying eggs with the oviposition substrate of Orius ovum, under 25 ± 1 ℃, RH=75 ± 5%, 16L:8D condition, cultivate, can hatch in ovum 5-6 days, transfer to nymph in the plastics box for feeding pests of side opening every day with dactylethrae, the corrugated paper fringe is placed in the box for feeding pests bottom, fresh clean kidney bean seedling, drosophila larvae and feed box thereof are placed on the corrugation paper slip, and the drosophila larvae feed that more renewed in 2-3 days enters the adult stage after Orius experiences 4-5 the length of time stand-by.
2. the raising method of Orius according to claim 1 is characterized in that: the Orius that adopts the drosophila larvae raising 1-2 age in days of 1 age in days; Adopt the Orius of the drosophila larvae raising 3-5 age in days of 2-3 age in days.
3. the raising method of Orius according to claim 2, it is characterized in that: described drosophila larvae cultural method mainly comprises the steps:
(1) configuration of medium: by weight, main component comprises: 600 parts in water, sugared 20-25 part, agar 8-10 part, corn flour or oatmeal 40-50 part, preservative 0.5-1 part, dusty yeast 10-15 part;
Its preparation method is: level is divided into two parts, a agar and the sugar of adding, another part adds corn flour or oatmeal, respectively heating and constantly stirring, treat that agar and sugar dissolve in water and is solution than thickness, corn flour or oatmeal dissolve in water and evenly distribute in the pasty state, again with the two admixed together stirring, slowly are heated to and boil, and keep boiling state 5 minutes, the medium that is homogeneous, stopped heating, slowly cooling;
When treating that the medium temperature is down to 74-76 ℃, add the preservative of formula ratio, stir, and divide while hot the Cans that install to sterilization;
Medium cools off but before not solidifying, spreads one deck dusty yeast on medium, to absorb the moisture on top layer, uses the bottle cap with thin copper mesh to seal bottleneck, and the medium cooled and solidified can be stand-by;
(2) raising of fruit bat: open medium bottleneck for subsequent use, clean a bottle inwall with 70% cotton ball soaked in alcohol, dry in the rear bottle and access 10-15 to kind of a fly, build bottle cap, be placed under 25 ± 1 ℃, RH=75 ± 5%, 16L:8D illumination condition and cultivated 24 hours, female fly post-coitum produces ovum in medium, will plant the fly taking-up after laying eggs and put into other blake bottle, hatches in the insectary approximately with the medium of ovum and can obtain larva in 24 hours;
Larva hatches rear raising 2-3 days, adding distil water to 2/3 place in the blake bottle, and larva climbs out of to enter in the water body and with screen filtration from medium isolates larva;
With the tweezers that go sharp passivation with the cotton ball soaked in alcohol sterilization after, drosophila larvae is taken out from feed, put into 0-4 ℃ of refrigerating box refrigeration 15-20 minute, put into the box for feeding pests of raising Orius after reducing the mobility of larva.
4. the raising method of Orius according to claim 3 is characterized in that: described preservative is a kind of in sorbic acid, propionic acid or the benzoic acid.
5. the raising method of Orius according to claim 3 is characterized in that: described sugar is a kind of in soft white sugar or the brown sugar.
6. the raising method of Orius according to claim 1, it is characterized in that: the Nutrition Soil described in the step (1) is that 1:1:1 mixes with black earth, vermiculite and turfy soil according to volume ratio.
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| CN103444632B (en) * | 2012-06-01 | 2015-12-09 | 株式会社易可盈 | Utilize the raising method of the predator of large wax moth ovum |
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