CN107279475A - A kind of method using the prey artificial feeding predatism stinkbug kept in cold storage - Google Patents

A kind of method using the prey artificial feeding predatism stinkbug kept in cold storage Download PDF

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Publication number
CN107279475A
CN107279475A CN201710681834.7A CN201710681834A CN107279475A CN 107279475 A CN107279475 A CN 107279475A CN 201710681834 A CN201710681834 A CN 201710681834A CN 107279475 A CN107279475 A CN 107279475A
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China
Prior art keywords
cold storage
kept
plant
stinkbug
prey
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CN201710681834.7A
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Chinese (zh)
Inventor
郭义
陈红印
张礼生
刘晨曦
王孟卿
唐艺婷
潘明真
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Institute of Plant Protection of Chinese Academy of Agricultural Sciences
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Institute of Plant Protection of Chinese Academy of Agricultural Sciences
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Priority to CN201710681834.7A priority Critical patent/CN107279475A/en
Publication of CN107279475A publication Critical patent/CN107279475A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/90Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/36Freezing; Subsequent thawing; Cooling

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  • Life Sciences & Earth Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Animal Husbandry (AREA)
  • Biotechnology (AREA)
  • Physiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Nutrition Science (AREA)
  • Insects & Arthropods (AREA)
  • Birds (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present invention relates to a kind of method using the prey artificial feeding predatism stinkbug kept in cold storage, comprise the following steps:Plant-feed insect is kept in cold storage, the plant-feed insect kept in cold storage is fed into predatism stinkbug after defrosting.Of the right age plant-feed insect is saved for a long time using low-temperature freezing; to feed predatism stinkbug; or when plant-feed insect live body is insufficient; the plant-feed insect for taking out freezing is supplemented; to ensure the lasting supply of predatism stinkbug food; provided safeguard for biological control predatism stinkbug, and it is cost-effective.

Description

A kind of method using the prey artificial feeding predatism stinkbug kept in cold storage
Technical field
Caught the invention belongs to the raising field of predatism stinkbug, more particularly to the prey artificial feeding that a kind of use keeps in cold storage The method of feeding habits stinkbug.
Background technology
Predatism stinkbug is a class omnivorousness or carnivorous natural enemy insect, can prey on the various pests of harm agriculture and forestry, such as Lepidopterous larvae, coleoptera larvae and adult, aphid, leafhopper and thrips etc..Therefore, predatism stinkbug has many types to be agricultural evil The Important Natural Enemy of worm, is used for biological control, meets the demand for development of green agriculture, with very big economic value and Ecological Effect Benefit.China's predatory bug insect resources enriches, and developable potentiality are very big, such as Candle-sticks stinkbugs, beneficial stinkbug, hunts stinkbug, Harpactor fuscipes, cigarette Fleahopper, Orius etc. have been applied to the insect on preventing and treating crops and forest, and pest control effect is notable.
The method of current artificial feeding predatism stinkbug is divided into two classes, and one is lived with the plant-feed insect of natural or artificial feeding Body raises predatism stinkbug, such as Harpactor fuscipes and beneficial stinkbug;Two be directly to raise predatism stinkbug using man-made feeds, and such as East Asia is small Flower stinkbug and Candle-sticks stinkbugs.
There is very big problem in actual large-scale production in two methods.The former is related to tritrophic interactions, phytophagous Quantity has certain proportionate relationship between insect and predatory bug, and plant-feed insect will typically develop certain age Predatism stinkbug could be fed, otherwise it is excessive or it is too small can increase feeding cost, appetite also not phase of the predatism stinkbug in different larval instar Together, in actual feeding process, the shortage of plant-feed insect live body or superfluous situation often occurs, predatism stinkbug can be caused Inanition, population quantity increasess slowly, or food surplus forms the wasting of resources.Predatism is directly raised using man-made feeds Stinkbug is simple and convenient, but the successful case that China individually raises predation stinkbug progress large-scale production using man-made feeds is few, mainly Reason is that the proportioning of each nutritional ingredient in man-made feeds is still defective, causes predatism stinkbug death rate height, the phenomenon of population deterioration, The man-made feeds general currently without most of predatism stinkbugs are adapted to, and the utilization rate of man-made feeds is low, cost of material is high, easy Rotten, technology of the package is complicated.So in actual production, it is still to use plant-feed insect live body method for breeding more.
The content of the invention
In view of the problems of prior art, the present invention provides a kind of using the prey artificial feeding kept in cold storage predation The method of property stinkbug, is saved of the right age plant-feed insect using low-temperature freezing for a long time, to feed predatism stinkbug, or When plant-feed insect live body is insufficient, the plant-feed insect for taking out freezing is supplemented, to ensure holding for predatism stinkbug food Continuous supply, the food security of abundance is provided for biological control predatism stinkbug.
The technical scheme that the present invention solves above-mentioned technical problem is as follows:
A kind of method using the prey artificial feeding predatism stinkbug kept in cold storage, comprises the following steps:By phytophagous elder brother The chilled storage of worm, predatism stinkbug is fed by the plant-feed insect kept in cold storage after defrosting.
The beneficial effects of the invention are as follows:
The study find that, when plant-feed insect live body is insufficient, the prey that predatism stinkbug takes food freezing can also complete Normally grow.So can be in suitable opportunity (when such as summer or man-made feeds are sufficient) mass rearing phytophagous elder brother Worm, is then saved of the right age plant-feed insect using low-temperature freezing, to feed predatism stinkbug, or in herbivore for a long time Property insect live body it is insufficient when, the plant-feed insect for taking out freezing is supplemented, to ensure the lasting confession of predatism stinkbug food Should, the food security of abundance is provided for biological control predatism stinkbug.Its principle such as government keeps in cold storage a part of meat, Suitable opportunity, which delivers, arrives in the market.Under cryogenic, most microorganisms are irreproducible, thus, in cryogenic freezing bar Under part plant-feed insect also imputrescibility go bad;Again because polypide is smaller, easily thawed after taking-up, feeding habits of being caught stinkbug sucks dry quickly Only, it is fully utilized before rotten.
The plant-feed insect of freezen protective is fed into predatism stinkbug after defrosting, is conducive to stimulating taking food for predatism stinkbug. If not thawing, the lancet of predatism stinkbug can not be pierced in suction polypide immediately, and reduce predatism stinkbug takes food enthusiasm.
On the basis of above-mentioned technical proposal, the present invention can also do following improvement.
Further, before keeping in cold storage, in addition to using hot water scald plant-feed insect the step of.
It is using the beneficial effect of such scheme:To prevent the polypide melanism of later stage plant-feed insect, if polypide melanism It will likely result in the problem of rotten speed is accelerated.
Further, the scald condition of plant-feed insect of hot water is:Scalded 5-10 minutes using 40 DEG C of -70 DEG C of hot water.
It is using the beneficial effect of such scheme:Suitable temperature and time can make melanism enzyme lose vigor, it is to avoid The generation of polypide melanism problem.
Further, after plant-feed insect is scalded with hot water, plant-feed insect is spread out and dries or dry up polypide surface water Point.
It is using the beneficial effect of such scheme:Probability mutually cohesive between polypide when advantageously reducing freezing.
Further, the plant-feed insect is to raise to obtain using man-made feeds or plant, and its age is suitable to raise Predatism stinkbug is supported, through collecting, cleaning and drying body surface moisture solution.
It is using the beneficial effect of such scheme:Suitable age can reduce feeding cost, and cleaning can remove polypide Surface excrement and swill.
Further, the temperature kept in cold storage is -10 DEG C to -80 DEG C.
It is using the beneficial effect of such scheme:Long-term storage is conducive to using above-mentioned cryogenic temperature, if temperature mistake Height is easily caused rotten rotten problem, and temperature is too low, wastes power cost.
Further, the specific species of the plant-feed insect is lepidopteran insect larvae, such as mythimna separata, corn borer, twill night Moth, rice moth, greenish brown hawk moth and bollworm etc..
It is using the beneficial effect of such scheme:Predatism stinkbug likes taking food the larva of lepidopterous insects.
Further, the age of the plant-feed insect is 2-5 ages.Suitable age can be selected depending on the species of predatism stinkbug Phase.
It is using the beneficial effect of such scheme:Predatism stinkbug is different in different larval instar content, the age of plant-feed insect Difference, body size is different, and suitable age advantageously reduces food waste, is conducive to growing for predatism stinkbug.
Further, the condition of feeding predatism stinkbug is:Temperature is 25 ± 1 DEG C, and humidity is 65 ± 5%, periodicity of illumination L:D= 14h:10h, wherein L write a Chinese character in simplified form for bright phase (Light's), and D writes a Chinese character in simplified form for dark phase (Dark's).
It is using the beneficial effect of such scheme:Be conducive to preying on growing for stinkbug using above-mentioned condition, if warm Spending the low developmental rate that is easily caused reduces, if temperature is too high to be easily caused death rate rising;Easily led if humidity is too low Predatism stinkbug body surface moisture content excessive vaporization is caused, if humidity is too high easy to induce germ;Hold if periodicity of illumination is improper It is easily caused predatism stinkbug growth rhythm disorderly.
Embodiment
The principles and features of the present invention are described below, and the given examples are served only to explain the present invention, is not intended to limit Determine the scope of the present invention.
Using the method for the prey artificial feeding predatism stinkbug kept in cold storage, comprise the following steps:Manually feed or plant Thing plant carrys out mass rearing plant-feed insect, when reaching the age that can feed predatism stinkbug, collects, and body surface is dried in cleaning Plant-feed insect, directly is fitted into container to be placed in -10 DEG C to -80 DEG C refrigerators and preserves for a long time by moisture content without hot water treatment.Make Used time takes out naturally to thaw or thawed with warm water, then feeds predatism stinkbug.
Using hot water plant-feed insect can also be scalded before freezing, specifically include following steps:First, employment Work feed or plant carry out mass rearing plant-feed insect, when reaching the age that can feed predatism stinkbug, collect, clearly Wash, scalded 5-10 minutes using 40 DEG C of -70 DEG C of hot water, to prevent later stage polypide melanism;Then spread out and dry or dry up polypide surface Moisture, freezing;Finally collect in a reservoir, be placed in -10 DEG C to -80 DEG C refrigerators and preserve for a long time.Naturally to thaw is taken out when using Or thawed with warm water, then feed predatism stinkbug.
It is specifically introduced below by some specific embodiments.
Embodiment 1
Manually feed raises mythimna separata, when armyworm larvae reached for 5 age, picks, and is eluriated with running water and removes excrement and food Residue, is put into the water-bath equipped with 60 DEG C of hot water and soaks 8 minutes, to prevent later stage polypide melanism, during immersion, stir 2-3 times, Then pull out to spread out and be placed on rapid airing on blotting paper, collect in plastic casing, be placed in freezen protective in -20 DEG C of refrigerators.Use When take out after expense, thaw at RT, deliver feeding Candle-sticks stinkbugs.Feeding condition is:Temperature is 25 ± 1 DEG C, and humidity is 65 ± 5%, Periodicity of illumination L:D=14h:10h.
Embodiment 2
Manually feed raises mythimna separata, when armyworm larvae reached for 2 age, picks, and is eluriated with running water and removes excrement and food Residue, is put into the water-bath equipped with 40 DEG C of hot water and soaks 10 minutes, to prevent later stage polypide melanism, during immersion, stir 2-3 It is secondary, then pull out to spread out and be placed on rapid airing on blotting paper, collect in plastic casing, be placed in freezen protective in -10 DEG C of refrigerators.Make Used time is taken out after expense, thaw at RT, delivers feeding Candle-sticks stinkbugs.Feeding condition is:Temperature be 25 ± 1 DEG C, humidity be 65 ± 5%, periodicity of illumination L:D=14h:10h.
Embodiment 3
Manually feed raises mythimna separata, when armyworm larvae reached for 3 age, picks, and is eluriated with running water and removes excrement and food Residue, is put into the water-bath equipped with 70 DEG C of hot water and soaks 5 minutes, to prevent later stage polypide melanism, during immersion, stir 2-3 times, Then pull out to spread out and be placed on rapid airing on blotting paper, collect in plastic casing, be placed in freezen protective in -80 DEG C of refrigerators.Use When take out after expense, thaw at RT, deliver feeding Candle-sticks stinkbugs.Feeding condition is:Temperature is 25 ± 1 DEG C, and humidity is 65 ± 5%, Periodicity of illumination L:D=14h:10h.
Embodiment 4
Manually feed raises mythimna separata, when armyworm larvae reached for 4 age, picks, and is eluriated with running water and removes excrement and food Residue, armyworm larvae is collected in plastic casing, body surface moisture content is dried, is placed in freezen protective in -20 DEG C of refrigerators.Taken out when using After expense, thaw at RT, feeding Candle-sticks stinkbugs are delivered.Feeding condition is:Temperature is 25 ± 1 DEG C, and humidity is 65 ± 5%, illumination week Phase L:D=14h:10h.
Experimental result
I.e. feeding freezes mythimna separata since Candle-sticks stinkbug egg hatchings, during to adult, the development time average out to of Candle-sticks stinkbugs 25.84 days, Body weight female adult pest is 87.44 milligrams, and male imago is 62.20 milligrams, the suitable (hair with feeding live body armyworm larvae under equal conditions Educate time average out to 25.57 days, body weight is that female adult pest is 84.51 milligrams, male imago is 63.70 milligrams), but compare experiment at present The development time that room is generally raised using tussah chrysalis is short 1.64 days (average out to 27.48 days);It is viscous that adult breeding period also feeds freezing Worm, its single head Adult worms producting eggs amount average out to 391.67, suitable (the single head adult with feeding live body armyworm larvae under equal conditions Egg laying amount average out to 406.21, statistically there was no significant difference, the Candle-sticks generally raised than current laboratory using tussah chrysalis Stinkbug egg laying amount is wanted high 61.15 (330.52).To sum up, Candle-sticks stinkbugs are raised with being raised using live body mythimna separata using the method for freezing mythimna separata Support Candle-sticks stinkbugs to compare with method, difference that there are no significant in terms of nymphal development time, adult body weight and Adult worms producting eggs amount, and adopt Cost of labor and feed cost can be saved with the method for freezing mythimna separata, because can be with the breeding mythimna separata of scale without to examine Consider the demand for whether being more than Candle-sticks stinkbugs, then unnecessary mythimna separata freezing is got up, supplemented on suitable opportunity.
So, it can raise predatism stinkbug (such as with the plant-feed insect (such as mythimna separata) kept in cold storage in actual production Candle-sticks stinkbugs), or the plant-feed insect that supplement feeding keeps in cold storage when live body plant-feed insect is insufficient, to meet predatism stinkbug The food supply of biological control, and it is cost-effective.
The foregoing is only presently preferred embodiments of the present invention, be not intended to limit the invention, it is all the present invention spirit and Within principle, any modification, equivalent substitution and improvements made etc. should be included in the scope of the protection.

Claims (10)

1. a kind of method using the prey artificial feeding predatism stinkbug kept in cold storage, it is characterised in that comprise the following steps:Will Plant-feed insect is kept in cold storage, and the plant-feed insect kept in cold storage is fed into predatism stinkbug after defrosting.
2. a kind of using the method for prey artificial feeding predatism stinkbug kept in cold storage according to claim 1, its feature exists In, before keeping in cold storage, in addition to using hot water scald plant-feed insect the step of.
3. a kind of using the method for prey artificial feeding predatism stinkbug kept in cold storage according to claim 2, its feature exists It is in the scald condition of plant-feed insect of, hot water:Scalded 5-10 minutes using 40 DEG C of -70 DEG C of hot water.
4. a kind of method using the prey artificial feeding predatism stinkbug kept in cold storage according to Claims 2 or 3, its feature It is, after plant-feed insect is scalded with hot water, plant-feed insect is spread out and dries or dry up polypide surface moisture.
5. according to a kind of any one of claim 1-3 methods using the prey artificial feeding predatism stinkbug kept in cold storage, Characterized in that, the plant-feed insect is to raise to obtain using man-made feeds or plant, and its age catches suitable for raising Feeding habits stinkbug, through collecting, cleaning and drying body surface moisture solution.
6. according to a kind of any one of claim 1-3 methods using the prey artificial feeding predatism stinkbug kept in cold storage, Characterized in that, the temperature kept in cold storage is -10 DEG C to -80 DEG C.
7. according to a kind of any one of claim 1-3 methods using the prey artificial feeding predatism stinkbug kept in cold storage, Characterized in that, the plant-feed insect is the larva of lepidopterous insects.
8. a kind of using the method for prey artificial feeding predatism stinkbug kept in cold storage according to claim 7, its feature exists In the plant-feed insect is mythimna separata, corn borer, prodenia litura, rice moth, greenish brown hawk moth or bollworm.
9. according to a kind of any one of claim 1-3 methods using the prey artificial feeding predatism stinkbug kept in cold storage, Characterized in that, the age of the plant-feed insect kept in cold storage is 2-5 ages.
10. according to a kind of any one of claim 1-3 methods using the prey artificial feeding predatism stinkbug kept in cold storage, Characterized in that, the condition of feeding predatism stinkbug is:Temperature is 25 ± 1 DEG C, and humidity is 65 ± 5%, periodicity of illumination L:D=14h: 10h。
CN201710681834.7A 2017-08-10 2017-08-10 A kind of method using the prey artificial feeding predatism stinkbug kept in cold storage Pending CN107279475A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109042542A (en) * 2018-09-12 2018-12-21 山东农业大学 A kind of method of meridic diet breeding Propylaea japonica
CN111066732A (en) * 2019-12-13 2020-04-28 河南省农业科学院烟草研究所 Method for breeding orius sauteri
CN114794019A (en) * 2021-06-01 2022-07-29 浙江省农业科学院 Method for breeding orius miri

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CN102125002A (en) * 2011-04-13 2011-07-20 中国农业科学院植物保护研究所 Method for artificially raising plant bug parasitic wasps
CN102293182A (en) * 2011-07-18 2011-12-28 山东省农业科学院植物保护研究所 Orius sauteri raising method
CN105309393A (en) * 2015-12-01 2016-02-10 贵州省植物保护研究所 Novel ecological system breeding method for orius minutus
CN106071055A (en) * 2016-08-25 2016-11-09 徐功达 Insecticide pellet refrigerating process
CN106306366A (en) * 2016-08-25 2017-01-11 徐功达 Live insect freezing technology

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102125002A (en) * 2011-04-13 2011-07-20 中国农业科学院植物保护研究所 Method for artificially raising plant bug parasitic wasps
CN102293182A (en) * 2011-07-18 2011-12-28 山东省农业科学院植物保护研究所 Orius sauteri raising method
CN105309393A (en) * 2015-12-01 2016-02-10 贵州省植物保护研究所 Novel ecological system breeding method for orius minutus
CN106071055A (en) * 2016-08-25 2016-11-09 徐功达 Insecticide pellet refrigerating process
CN106306366A (en) * 2016-08-25 2017-01-11 徐功达 Live insect freezing technology

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109042542A (en) * 2018-09-12 2018-12-21 山东农业大学 A kind of method of meridic diet breeding Propylaea japonica
CN109042542B (en) * 2018-09-12 2020-07-03 山东农业大学 Method for breeding propylaea japonica by using semi-artificial feed
CN111066732A (en) * 2019-12-13 2020-04-28 河南省农业科学院烟草研究所 Method for breeding orius sauteri
CN114794019A (en) * 2021-06-01 2022-07-29 浙江省农业科学院 Method for breeding orius miri

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Application publication date: 20171024