CN111066732A - Method for breeding orius sauteri - Google Patents
Method for breeding orius sauteri Download PDFInfo
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- CN111066732A CN111066732A CN201911284236.1A CN201911284236A CN111066732A CN 111066732 A CN111066732 A CN 111066732A CN 201911284236 A CN201911284236 A CN 201911284236A CN 111066732 A CN111066732 A CN 111066732A
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- 238000009395 breeding Methods 0.000 title claims abstract description 60
- 230000001488 breeding effect Effects 0.000 title claims abstract description 60
- 238000000034 method Methods 0.000 title claims abstract description 22
- 241001635523 Orius sauteri Species 0.000 title claims description 39
- 235000013601 eggs Nutrition 0.000 claims abstract description 71
- 241001477931 Mythimna unipuncta Species 0.000 claims abstract description 49
- 241000238631 Hexapoda Species 0.000 claims abstract description 48
- 241001635528 Orius minutus Species 0.000 claims abstract description 32
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 20
- 235000021332 kidney beans Nutrition 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 235000012907 honey Nutrition 0.000 claims description 15
- 229920000742 Cotton Polymers 0.000 claims description 9
- 230000017448 oviposition Effects 0.000 claims description 8
- 238000004519 manufacturing process Methods 0.000 claims description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 6
- 238000004321 preservation Methods 0.000 claims description 6
- 230000019617 pupation Effects 0.000 claims description 6
- 238000012216 screening Methods 0.000 claims description 6
- 239000002689 soil Substances 0.000 claims description 6
- 239000000758 substrate Substances 0.000 claims description 6
- 241001635529 Orius Species 0.000 claims description 5
- 240000008042 Zea mays Species 0.000 claims description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 5
- 240000008067 Cucumis sativus Species 0.000 claims description 4
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 claims description 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 4
- 235000005822 corn Nutrition 0.000 claims description 4
- 241001494191 Myxobolus Species 0.000 claims description 3
- 241001494184 Myxozoa Species 0.000 claims description 3
- 241000721621 Myzus persicae Species 0.000 claims description 3
- 238000012136 culture method Methods 0.000 claims description 3
- 238000005520 cutting process Methods 0.000 claims description 3
- 230000000249 desinfective effect Effects 0.000 claims description 3
- 230000032669 eclosion Effects 0.000 claims description 3
- 230000003203 everyday effect Effects 0.000 claims description 3
- 230000012447 hatching Effects 0.000 claims description 3
- 230000013011 mating Effects 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 238000005516 engineering process Methods 0.000 abstract description 4
- 235000016709 nutrition Nutrition 0.000 description 5
- 241001124076 Aphididae Species 0.000 description 4
- 241000238876 Acari Species 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 230000003031 feeding effect Effects 0.000 description 3
- 241000258937 Hemiptera Species 0.000 description 2
- 241001414989 Thysanoptera Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 235000013312 flour Nutrition 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 241000238421 Arthropoda Species 0.000 description 1
- 208000003643 Callosities Diseases 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000122098 Ephestia kuehniella Species 0.000 description 1
- 241000256257 Heliothis Species 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 241000255777 Lepidoptera Species 0.000 description 1
- 241001467460 Myxogastria Species 0.000 description 1
- 241000819999 Nymphes Species 0.000 description 1
- 241000595629 Plodia interpunctella Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 1
- 241001454295 Tetranychidae Species 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- MYSWGUAQZAJSOK-UHFFFAOYSA-N ciprofloxacin Chemical compound C12=CC(N3CCNCC3)=C(F)C=C2C(=O)C(C(=O)O)=CN1C1CC1 MYSWGUAQZAJSOK-UHFFFAOYSA-N 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
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- 150000004665 fatty acids Chemical class 0.000 description 1
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- 230000007774 longterm Effects 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 244000062645 predators Species 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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- Food Science & Technology (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Birds (AREA)
- Insects & Arthropods (AREA)
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Abstract
The invention relates to the field of plant protection and insect breeding technology, in particular to a method for breeding orius minutus by using armyworm eggs as feed.
Description
Technical Field
The invention belongs to the technical field of insect science, and particularly relates to a method for breeding orius sauteri.
Background
Orius sauteri (Poppius) belonging to family Hemiptera of family Oridonicaceae, Anthocidae, is an egg of many small arthropods (e.g., thrips, aphids, spider mites, whiteflies) and various lepidoptera insects (e.g., Heliothis gossypii, armyworms, etc.) and a natural enemy of their first-hatched larvae. Has the characteristics of early occurrence, large amount, long time, miscellaneous eating property, wide distribution, strong activity and the like. Therefore, the orius sauteri has extremely high application value as a natural predator insect of omnivory in a farmland ecosystem.
In order to improve the utilization value of the orius sauteri in the aspect of biological control, scholars at home and abroad research the aspects of life regularity, life habits, biological characteristics, growth and development of the orius sauteri, and particularly try to feed the orius sauteri by using plant source feeds such as artificial ooecium rubrum pupae, thrips, pest mites, aphids and the like, plant pollen, tender corns, beer yeast solution and the like in the aspect of the alternative feed of the orius sauteri, but the feeding effect of the orius sauteri is not ideal, and the large-scale feeding of the orius sauteri cannot be realized. In foreign countries, researchers have implemented large-scale breeding of orius parviflorus by using indian corn borer Plodia interpunctella eggs and mediterranean meal borer Ephestiakuehniella eggs as alternative feeds. Due to the propagation conditions and the limitations of host plants, the two lepidopteran insects do not form a breeding scale in China. The application of the orius minutus in preventing and controlling pests in China is relatively late, the technical research foundation in the aspects of feeding and releasing is still very weak, and no effective feed exists at present.
Aiming at various problems existing in the existing artificial feeding of orius sauteri, in order to better develop the orius sauteri and realize the large-scale feeding of the orius sauteri, the inventor provides a method for feeding the orius sauteri by taking armyworm eggs as feed through long-term experiments.
Disclosure of Invention
In order to solve the problems, the invention provides a method for breeding orius sauteri.
The technical scheme adopted by the invention is as follows:
a method for breeding orius sauteri adopts armyworm eggs as feed to breed the orius sauteri, and mainly comprises the following steps:
(1) selection of oviposition substrates: disinfecting fresh and complete kidney beans purchased in a supermarket by using alcohol on the surface, and putting the kidney beans in an insect breeding box for later use after the alcohol is completely volatilized;
(2) screening of orius sauteri species: breeding adult Oridous avidus collected from a cucumber field with myzus persicae in an insect breeding box to establish a stable population, screening 50 pairs of robust adults which are just emerged, enabling the adults to lay eggs after natural mating, and collecting eggs of the Oridous avidus as production insect seeds for later use;
(3) feeding orius miniatus nymphs: the orius minutus nymphs are raised in a bug breeding room with the temperature of 25 +/-1 ℃, the relative humidity of 70 +/-5% and the photoperiod of 16L:8D as armyworm eggs, the orius minutus nymphs are placed in a bug breeding box, and 5-10 pieces of fan-shaped filter paper are placed in the bug breeding box; each box contains about 500 nymphs, and the nymphs in the insect breeding boxes are consistent in age;
(4) feeding of orius sauteri adults: feeding adults in a phytotron with the temperature of 25 +/-1 ℃, the relative humidity of 70 +/-5% and the photoperiod of 16L:8D by using armyworm eggs, taking kidney beans as an egg laying substrate, putting adult orius minutus in an insect feeding box, and putting 5-10 pieces of fan-shaped filter paper in the insect feeding box, wherein each box contains about 500 adults;
(5) collecting the orius minutus eggs: and (3) moving out the kidney beans which have spawned every other day, transferring the kidney beans into an insect breeding box, placing the insect breeding box into an insect breeding chamber with the temperature of 25 +/-1 ℃, the relative humidity of 70 +/-5% and the photoperiod of 16L:8D for hatching, placing 3-5 kidney beans which have spawned in each box, and continuing to propagate.
The insect-culturing box in the steps (3) and (4) is a sealed plastic preservation box with the specification of 32cm multiplied by 22cm multiplied by 10cm, and the middle of the preservation box cover is sealed by a 100-mesh insect-proof net with the thickness of 15 cm multiplied by 10 cm; the filter paper is qualitative filter paper, is cut into strips, and the strips are folded into a fan shape and are placed in the east Asian orius minutus feeding box, so that the movement space is increased.
The armyworm eggs collected in the current day in the step (3) and the step (4) are used for feeding nymphs and adults of orius minutus, wherein egg strips are placed on the nymphs in an amount of 4 armyworm eggs per day, and egg strips are placed on the adults in an amount of 6 armyworm eggs per day.
The culture method of the myxobolus eggs comprises the following steps:
(1) collecting and feeding armyworm larvae: collecting armyworm larvae from corn, and feeding with artificial feed in an armyworm breeding room with temperature of 25 + -1 deg.C, relative humidity of 70 + -5% and photoperiod of 16L: 8D; breeding 1-3 instar larva in plastic basket, transferring into insect cage after 3 instar, and breeding until the larva is mature;
(2) culturing the armyworm pupae: transferring the mature nymphs into a tray paved with fine sandy soil with the thickness of 10cm, digging the nymphs out of the soil after 3 d, putting the nymphs into a pupation box for eclosion, and sealing the pupation box by using gauze for about 100 heads of each box;
(3) feeding adult myxozoa: feeding adult insects with 10% of honey water by volume ratio, feeding adult insects in an insect breeding cage, and replacing the honey water once a day;
(4) collecting the myxoworm eggs: randomly placing spawning ropes in an adult insect cage for raising adults in the spawning period, replacing the spawning ropes and honey water every day, collecting fresh armyworm eggs, and cutting the eggs into egg strips containing 10-20 eggs.
The size of the plastic basket in the step (1) is 20 cmX20 cm in diameter.
The step (3) of feeding with honey water is to drop honey water onto the absorbent cotton, the absorbent cotton is completely soaked, and the absorbent cotton is placed in a culture dish and then put into an adult feeding cage.
The invention has the beneficial effects that: the armyworm eggs utilized in the method can meet the nutritional requirements of the orius minutus in the whole growth period, the problems existing in the feeding of the orius minutus by using the flour mites, the aphids and the like as feeds are solved, the armyworm large-scale feeding technology is mature, the armyworm eggs are easy to obtain and store, the requirements on production environment are low and the like, the basis on the feeds is provided for the large-scale feeding of the orius minutus, and the feeding effect is good.
Detailed Description
A method for breeding orius sauteri adopts armyworm eggs as feed to breed the orius sauteri, and mainly comprises the following steps:
(1) selection of oviposition substrates: disinfecting fresh and complete kidney beans purchased in a supermarket by using alcohol on the surface, and putting the kidney beans in an insect breeding box for later use after the alcohol is completely volatilized;
(2) screening of orius sauteri species: breeding adult Oridous avidus collected from a cucumber field with myzus persicae in an insect breeding box to establish a stable population, screening 50 pairs of robust adults which are just emerged, enabling the adults to lay eggs after natural mating, and collecting eggs of the Oridous avidus as production insect seeds for later use;
(3) feeding orius miniatus nymphs: the orius minutus nymphs are raised in a bug breeding room with the temperature of 25 +/-1 ℃, the relative humidity of 70 +/-5% and the photoperiod of 16L:8D as armyworm eggs, the orius minutus nymphs are placed in a bug breeding box, and 5-10 pieces of fan-shaped filter paper are placed in the bug breeding box; each box contains about 500 nymphs, and the nymphs in the insect breeding boxes are consistent in age;
(4) feeding of orius sauteri adults: feeding adults in a phytotron with the temperature of 25 +/-1 ℃, the relative humidity of 70 +/-5% and the photoperiod of 16L:8D by using armyworm eggs, taking kidney beans as an egg laying substrate, putting adult orius minutus in an insect feeding box, and putting 5-10 pieces of fan-shaped filter paper in the insect feeding box, wherein each box contains about 500 adults;
(5) collecting the orius minutus eggs: and (3) moving out the kidney beans which have spawned every other day, transferring the kidney beans into an insect breeding box, placing the insect breeding box into an insect breeding chamber with the temperature of 25 +/-1 ℃, the relative humidity of 70 +/-5% and the photoperiod of 16L:8D for hatching, placing 3-5 kidney beans which have spawned in each box, and continuing to propagate.
The insect-culturing box in the steps (3) and (4) is a sealed plastic preservation box with the specification of 32cm multiplied by 22cm multiplied by 10cm, and the middle of the preservation box cover is sealed by a 100-mesh insect-proof net with the thickness of 15 cm multiplied by 10 cm; the filter paper is qualitative filter paper, is cut into strips, and the strips are folded into a fan shape and are placed in the east Asian orius minutus feeding box, so that the movement space is increased.
The armyworm eggs collected in the current day in the step (3) and the step (4) are used for feeding nymphs and adults of orius minutus, wherein egg strips are placed on the nymphs in an amount of 4 armyworm eggs per day, and egg strips are placed on the adults in an amount of 6 armyworm eggs per day.
When the armyworm eggs are adopted to feed the orius minutus, the collected armyworm eggs are used for feeding the nymphs and adults of the orius minutus; the design is mainly that the armyworm eggs collected on the same day have the most sufficient nutrients, and can well meet the growth and development nutritional requirements of the orius minutus.
The oviposition matrix used in the step (1) is the whole complete kidney bean, 1-year-old and 2-year-old orius minutus hardly eat, and the kidney bean pods are absorbed by the thorns to supplement water.
The orius sauteri originally adopted in the step (2) is collected from a cucumber field.
The culture method of the myxobolus eggs comprises the following steps:
(1) collecting and feeding armyworm larvae: collecting armyworm larvae from corn, and feeding with artificial feed in an armyworm breeding room with temperature of 25 + -1 deg.C, relative humidity of 70 + -5% and photoperiod of 16L: 8D; breeding 1-3 instar larva in plastic basket, transferring into insect cage after 3 instar, and breeding until the larva is mature;
(2) culturing the armyworm pupae: transferring the mature nymphs into a tray paved with fine sandy soil with the thickness of 10cm, digging the nymphs out of the soil after 3 d, putting the nymphs into a pupation box for eclosion, and sealing the pupation box by using gauze for about 100 heads of each box;
(3) feeding adult myxozoa: feeding adult insects with 10% of honey water by volume ratio, feeding adult insects in an insect breeding cage, and replacing the honey water once a day;
(4) collecting the myxoworm eggs: randomly placing spawning ropes in an adult insect cage for raising adults in the spawning period, replacing the spawning ropes and honey water every day, collecting fresh armyworm eggs, and cutting the eggs into egg strips containing 10-20 eggs.
The size of the plastic basket in the step (1) is 20cm multiplied by 20cm in diameter.
The step (3) of feeding the honey water refers to that the honey water is dripped on the absorbent cotton, the absorbent cotton is completely soaked, and the absorbent cotton is placed in a culture dish and is placed in an adult feeding cage.
The insect breeding cage used in the feeding and collecting of the myxomatosis eggs selects a 100-mesh insect-proof net to be sewn into a gauze with the specification of 60cm multiplied by 60cm, and takes a solid material as a frame.
The invention adopts armyworm eggs as feed to feed orius sauteri, and has the following beneficial effects:
1. the armyworm is used as a feed for a plurality of natural enemy insects, the requirement on the rising environment is low, the production cost is low, the growth and development are not influenced by seasons, the artificial feeding technology is mature, the mass production of armyworm eggs is easy to realize, and the armyworm egg feed can be a feed for a long time for raising orius minutus;
2. the armyworm eggs contain rich nutritional ingredients such as protein, amino acid and fatty acid, can meet the nutritional requirements of the orius minutus in the whole growth period, and realize large-scale breeding of the orius minutus.
In conclusion, the armyworm eggs utilized in the method can meet the nutritional requirements of the orius sauteri in the whole growth period, the problems existing in the feeding of the orius sauteri by utilizing the flour mites, the aphids and the like as the feed are solved, the characteristics that the armyworm large-scale feeding technology is mature, the armyworm eggs are easy to obtain and store, the requirement on the production environment is low and the like are utilized, the basis on the feed is provided for the large-scale feeding of the orius sauteri, and the feeding effect is good.
Example 1
Feeding orius miniatus nymphs with mythic eggs:
the nymph stage of the orius minutus bred by the myxomycete egg is 13.7d, and the survival rate of the orius minutus bred from the low age to the old age is as follows: nymphs at 1 st (94%), nymphs at 2 st (97%), nymphs at 3 st (96%), nymphs at 4 st (98%), nymphs at 5 th (99%).
Example 2
Feeding the adult orius sauteri with myxoid eggs:
the average life of the adult Ornitus parvifolius bred by the myxozoon ovum is 17.1d, and the average egg laying amount per female is 72.5.
Claims (6)
1. A method for breeding orius sauteri is characterized by comprising the following steps: the method for feeding orius sauteri by using the myxoworm eggs as the feed mainly comprises the following steps:
(1) selection of oviposition substrates: disinfecting fresh and complete kidney beans purchased in a supermarket by using alcohol on the surface, and putting the kidney beans in an insect breeding box for later use after the alcohol is completely volatilized;
(2) screening of orius sauteri species: breeding adult Oridous avidus collected from a cucumber field with myzus persicae in an insect breeding box to establish a stable population, screening 50 pairs of robust adults which are just emerged, enabling the adults to lay eggs after natural mating, and collecting eggs of the Oridous avidus as production insect seeds for later use;
(3) feeding orius miniatus nymphs: the orius minutus nymphs are raised in a bug breeding room with the temperature of 25 +/-1 ℃, the relative humidity of 70 +/-5% and the photoperiod of 16L:8D as armyworm eggs, the orius minutus nymphs are placed in a bug breeding box, and 5-10 pieces of fan-shaped filter paper are placed in the bug breeding box; each box contains about 500 nymphs, and the nymphs in the insect breeding boxes are consistent in age;
(4) feeding of orius sauteri adults: feeding adults in a phytotron with the temperature of 25 +/-1 ℃, the relative humidity of 70 +/-5% and the photoperiod of 16L:8D by using armyworm eggs, taking kidney beans as an egg laying substrate, putting adult orius minutus in an insect feeding box, and putting 5-10 pieces of fan-shaped filter paper in the insect feeding box, wherein each box contains about 500 adults;
(5) collecting the orius minutus eggs: and (3) moving out the kidney beans which have spawned every other day, transferring the kidney beans into an insect breeding box, placing the insect breeding box into an insect breeding chamber with the temperature of 25 +/-1 ℃, the relative humidity of 70 +/-5% and the photoperiod of 16L:8D for hatching, placing 3-5 kidney beans which have spawned in each box, and continuing to propagate.
2. The method for breeding orius sauteri according to claim 1, wherein the method comprises the following steps: the insect-culturing box in the steps (3) and (4) is a sealed plastic preservation box with the specification of 32cm multiplied by 22cm multiplied by 10cm, and the middle of the preservation box cover is sealed by a 100-mesh insect-proof net with the thickness of 15 cm multiplied by 10 cm; the filter paper is qualitative filter paper, is cut into strips, and the strips are folded into a fan shape and are placed in the east Asian orius minutus feeding box, so that the movement space is increased.
3. The method for breeding orius sauteri according to claim 1, wherein the method comprises the following steps: the armyworm eggs collected in the current day in the step (3) and the step (4) are used for feeding nymphs and adults of orius minutus, wherein egg strips are placed on the nymphs in an amount of 4 armyworm eggs per day, and egg strips are placed on the adults in an amount of 6 armyworm eggs per day.
4. The method for breeding orius sauteri according to claim 1, wherein the method comprises the following steps: the culture method of the myxobolus eggs comprises the following steps:
(1) collecting and feeding armyworm larvae: collecting armyworm larvae from corn, and feeding with artificial feed in an armyworm breeding room with temperature of 25 + -1 deg.C, relative humidity of 70 + -5% and photoperiod of 16L: 8D; breeding 1-3 instar larva in plastic basket, transferring into insect cage after 3 instar, and breeding until the larva is mature;
(2) culturing the armyworm pupae: transferring the mature nymphs into a tray paved with fine sandy soil with the thickness of 10cm, digging the nymphs out of the soil after 3 d, putting the nymphs into a pupation box for eclosion, and sealing the pupation box by using gauze for about 100 heads of each box;
(3) feeding adult myxozoa: feeding adult insects with 10% of honey water by volume ratio, feeding adult insects in an insect breeding cage, and replacing the honey water once a day;
(4) collecting the myxoworm eggs: randomly placing spawning ropes in an adult insect cage for raising adults in the spawning period, replacing the spawning ropes and honey water every day, collecting fresh armyworm eggs, and cutting the eggs into egg strips containing 10-20 eggs.
5. The method for breeding orius sauteri according to claim 4, wherein the method comprises the following steps: the size of the plastic basket in the step (1) is 20 cmX20 cm in diameter.
6. The method for breeding orius sauteri according to claim 4, wherein the method comprises the following steps: the step (3) of feeding with honey water is to drop honey water onto the absorbent cotton, the absorbent cotton is completely soaked, and the absorbent cotton is placed in a culture dish and then put into an adult feeding cage.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112997972A (en) * | 2021-03-30 | 2021-06-22 | 河南省农业科学院烟草研究所 | Ornitus avidus egg preservation method suitable for large-scale breeding |
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