CN112471082A - Broad-band optimized aphid fly breeding method - Google Patents
Broad-band optimized aphid fly breeding method Download PDFInfo
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- CN112471082A CN112471082A CN202011496048.8A CN202011496048A CN112471082A CN 112471082 A CN112471082 A CN 112471082A CN 202011496048 A CN202011496048 A CN 202011496048A CN 112471082 A CN112471082 A CN 112471082A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a breeding method of broad-band superior aphid flies. The wide-band optimal aphid fly breeding method provided by the invention is suitable for artificial large-scale breeding of wide-band optimal aphid flies, the death rate of the wide-band optimal aphid fly breeding process can be reduced, the pupation rate and the eclosion rate of the wide-band optimal aphid flies are improved, and the survival rate of the wide-band optimal aphid fly larvae and imagoes in the long-term breeding process can reach 95%.
Description
Technical Field
The invention relates to the field of artificial large-scale breeding of natural enemy insects, in particular to a wide-band excellent aphid fly breeding method.
Background
Predatory natural enemy insects are widely applied to biological control of agricultural and forestry pests, and play a great role in protecting the yield and quality safety of agricultural and forestry products and protecting the environment from pesticide pollution.
The wide-band excellent aphid fly can control aphids on various agricultural and forestry crops, and is a good predatory natural enemy insect.
At present, the artificial breeding of the broad band superior aphid flies is mainly to feed the aphids, but the breeding of the aphids is labor-consuming and time-consuming, the labor cost is high, and the temperature and humidity regulation and control difficulty in the large-scale breeding process of the aphids is high, so that the aphids are easily parasitized.
Compared with aphid feeding, the rice moth larva feeding technology is simple, the feeding cost is low, and large-scale feeding can be realized in any area and laboratory.
Disclosure of Invention
In view of the problems, the invention provides a prey and a feeding method which are more suitable for artificial large-scale feeding of wide-band excellent aphid flies, the prey and the feeding method can reduce the death rate of the wide-band excellent aphid flies in the breeding process, improve the pupation rate and the eclosion rate of the wide-band excellent aphid flies, and obtain more natural enemy commodities.
In order to achieve the purpose, the invention adopts the following technical scheme:
a broad-band optimal aphid fly breeding method is used for breeding broad-band optimal aphid flies by taking rice moth larvae as food sources.
Preferably, the breeding method of the rice moth larvae comprises the following steps: the rice moth larvae are obtained by breeding the rice moth with the corn meal and the wheat bran in a weight ratio of 1:2, and the breeding temperature is 26 +/-1 ℃.
Preferably, the 23-year-old broad-band excellent aphid flies are bred by taking rice moth larvae as a food source.
Preferably, the breeding method comprises the following steps:
(1) breeding rice moth larvae;
(2) wild collection and breeding of wide-band excellent aphids: collecting wild adult wide-band myzus persicae, putting into an insect rearing cage I, and feeding with 10% honey water and rape pollen;
(3) broad band optimal aphid fly oviposition pairing: placing the tobacco plant with the myzus persicae in an insect rearing cage II, then inoculating 1 pair of adult wide-band superior myzus persicae, and collecting egg granules every day when the wide-band superior myzus persicae lay eggs;
(4) hatching wide-band excellent aphid fly eggs: after the wide-band superior aphid flies lay eggs, taking out wide-band superior aphid fly eggs, placing the wide-band superior aphid fly eggs into an insect breeding box padded with filter paper, and placing the wide-band superior aphid fly eggs into an artificial climate box for hatching;
(5) 1-year-old wide-band excellent aphid fly breeding: when wide-band superior aphid fly eggs begin to hatch, putting myzus persicae into a culture dish for feeding, and adding myzus persicae every day;
(6) feeding wide-band excellent aphid flies of 2-3 ages: after the wide-band superior aphid flies grow to 2 years old, throwing the rice moth larvae into a wide-band superior aphid fly culture box according to the standard that 8-12 heads of each head of the wide-band superior aphid flies are fed by the wide-band superior aphid flies, and replacing the rice moth larvae every day;
(7) collecting and eclosion of broad band superior aphid fly pupae: and after the broad-band superior aphid flies pupate, taking out the broad-band superior aphid fly pupae, putting the broad-band superior aphid fly pupae into an insect breeding cage III, then putting the broad-band superior aphid fly pupae into an artificial climate chamber, and waiting for the broad-band superior aphid flies to eclosion.
Preferably, the feeding temperature of the wide-band optimized aphid fly is 26 +/-1 ℃.
Preferably, the length, width and height of the first insect cage and the second insect cage are both 120cm by 120 cm; the length, width and height of the insect cage III are 30cm x 30cm-60cm x 60 cm.
Preferably, the number of the individual myzus persicae in the tobacco plant with myzus persicae in the step (2) is more than 3000.
Preferably, in the step (4), myzus persicae is put into the culture dish according to the ratio of the number of the wide-band excellent aphid flies to the number of the myzus persicae of 1:100-1: 150.
Compared with the prior art, the invention has the beneficial effects that:
1. the rice moth larvae are rich in nutrition, and compared with aphids, the rice moth larvae can also be used as a living feed for breeding broad-band superior aphid flies, the rice moth larvae can be used as food for the broad-band superior aphid flies to ensure that the growth and development of the broad-band superior aphid flies are completed, and the survival rate of the broad-band superior aphid fly larvae and adults in the long-term feeding process can reach 95%.
2. The rice moth is bred by adopting the corn flour and the wheat bran in a weight ratio of 1:1.5-1:2.5, the corn flour and the wheat bran can be directly purchased on the market, a large number of plants for breeding aphids are not needed, the manual input cost is low, and the labor cost for breeding the broad-band excellent aphids can be reduced.
3. The breeding technology of the rice moth larvae is simple, and the rice moth larvae can be bred in a large scale in a short period.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
(1) Breeding rice moth larvae: taking corn flour and wheat bran in a weight ratio of 1:2 as food, carrying out artificial feeding on rice moths in a rice moth feeding box, wherein the feeding temperature is 26 +/-1 ℃, after the rice moths fed in the rice moth feeding box lay eggs, after rice moth eggs are incubated for 24 hours, screening out rice moth larvae, putting the rice moth larvae into a plastic box for later use, placing the plastic box in which the rice moth larvae are placed in a low-temperature environment for cold storage, wherein the cold storage temperature is 4 ℃, the cold storage humidity is 40%, and the storage time is less than or equal to 48 hours;
(2) wild collection of wide-band optimized aphids: collecting adult wide-band superior aphid flies in the field by using an insect catching net, and feeding the adult wide-band superior aphid flies indoors in an insect raising cage with the length, width and height of 120cm x 120cm by using 10% of honey water and rape pollen at the feeding temperature of 26 +/-1 ℃;
(3) broad band optimal aphid fly oviposition pairing: putting a tobacco plant with more than 3000 aphids in an insect rearing cage with the length, width and height of 120cm x 120cm, then inoculating 1 pair of adult wide-band excellent aphids, collecting egg grains every day when the wide-band excellent aphids lay eggs, and rearing at the temperature of 26 +/-1 ℃;
(4) hatching wide-band excellent aphid fly eggs: after wide-band excellent aphid flies lay eggs, taking the wide-band excellent aphid fly eggs out of an insect breeding box filled with filter paper, and placing the wide-band excellent aphid fly eggs into an artificial climate box for hatching, wherein the breeding temperature is 26 +/-1 ℃;
(5) 1-year-old wide-band excellent aphid fly breeding: when wide-band superior aphid fly eggs begin to hatch, feeding myzus persicae in a culture dish according to the ratio of the wide-band superior aphid flies to the myzus persicae of 1:150, and adding the myzus persicae every day, wherein the feeding temperature is 26 +/-1 ℃;
(6) feeding wide-band excellent aphid flies of 2-3 ages: after the wide-band superior aphid flies grow to 2 years old, taking out the rice moth larvae, putting the rice moth larvae into a wide-band superior aphid fly cultivation box according to the standard that 10 heads of each head of the superior aphid flies are used for feeding the wide-band superior aphid flies, and replacing the rice moth larvae every day, wherein the feeding temperature is 26 +/-1 ℃;
(7) collecting and eclosion of broad band superior aphid fly pupae: and after the wide-band superior aphid flies pupate, taking out the wide-band superior aphid fly pupae, placing the wide-band superior aphid fly pupae into an insect raising cage with the length, width and height of 30cm x 30cm, then placing the cage into an artificial climate chamber, and waiting for the wide-band superior aphid flies to eclose.
The number of samples in this example is 300, and 3 times of repetition are performed, and the pupation rate is 95%, the pupation rate is 100%, and the survival rate is 95% for each 100 repeated samples.
Example 2
(1) Breeding rice moth larvae: taking corn flour and wheat bran with a weight ratio of 1:1.5 as food, carrying out artificial feeding on rice moths in a rice moth feeding box, wherein the feeding temperature is 26 +/-1 ℃, after the rice moths fed in the rice moth feeding box lay eggs, after rice moth eggs are incubated for 24 hours, screening out rice moth larvae, putting the rice moth larvae into a plastic box for later use, placing the plastic box in which the rice moth larvae are placed in a low-temperature environment for cold storage, wherein the cold storage temperature is 4 ℃, the cold storage humidity is 40%, and the storage time is less than or equal to 48 hours;
(2) wild collection of wide-band optimized aphids: collecting adult wide-band superior aphid flies in the field by using an insect catching net, and feeding the adult wide-band superior aphid flies indoors in an insect raising cage with the length, width and height of 120cm x 120cm by using 10% of honey water and rape pollen at the feeding temperature of 26 +/-1 ℃;
(3) broad band optimal aphid fly oviposition pairing: putting a tobacco plant with more than 3000 aphids in an insect rearing cage with the length, width and height of 120cm x 120cm, then inoculating 1 pair of adult wide-band excellent aphids, collecting egg grains every day when the wide-band excellent aphids lay eggs, and rearing at the temperature of 26 +/-1 ℃;
(4) hatching wide-band excellent aphid fly eggs: after wide-band excellent aphid flies lay eggs, taking the wide-band excellent aphid fly eggs out of an insect breeding box filled with filter paper, and placing the wide-band excellent aphid fly eggs into an artificial climate box for hatching, wherein the breeding temperature is 26 +/-1 ℃;
(5) 1-year-old wide-band excellent aphid fly breeding: when wide-band superior aphid fly eggs begin to hatch, feeding myzus persicae in a culture dish according to the ratio of the wide-band superior aphid flies to the myzus persicae of 1:150, and adding the myzus persicae every day, wherein the feeding temperature is 26 +/-1 ℃;
(6) feeding wide-band excellent aphid flies of 2-3 ages: after the wide-band superior aphid flies grow to 2 years old, taking out the rice moth larvae, putting the rice moth larvae into a wide-band superior aphid fly cultivation box according to the standard that 5 heads of each head of the superior aphid flies are fed, and replacing the rice moth larvae every day, wherein the feeding temperature is 26 +/-1 ℃;
(8) collecting and eclosion of broad band superior aphid fly pupae: and after the wide-band superior aphid flies pupate, taking out the wide-band superior aphid fly pupae, placing the wide-band superior aphid fly pupae into an insect raising cage with the length, width and height of 60cm x 60cm, then placing the cage into an artificial climate chamber, and waiting for the wide-band superior aphid flies to eclose.
The number of samples in this example is 300, and 3 times of repetition are performed, and the pupation rate is 92%, the pupation rate is 100%, and the survival rate is 92% when the number of samples per repetition is 100.
Example 3
(1) Breeding rice moth larvae: taking corn flour and wheat bran with a weight ratio of 1:2.5 as food, carrying out artificial feeding on rice moths in a rice moth feeding box, wherein the feeding temperature is 26 +/-1 ℃, after the rice moths fed in the rice moth feeding box lay eggs, after rice moth eggs are incubated for 24 hours, screening out rice moth larvae, putting the rice moth larvae into a plastic box for later use, placing the plastic box in which the rice moth larvae are placed in a low-temperature environment for cold storage, wherein the cold storage temperature is 4 ℃, the cold storage humidity is 40%, and the storage time is less than or equal to 48 hours;
(2) wild collection of wide-band optimized aphids: collecting adult wide-band superior aphid flies in the field by using an insect catching net, and feeding the adult wide-band superior aphid flies indoors in an insect raising cage with the length, width and height of 120cm x 120cm by using 10% of honey water and rape pollen at the feeding temperature of 26 +/-1 ℃;
(3) broad band optimal aphid fly oviposition pairing: putting a tobacco plant with more than 3000 aphids in an insect rearing cage with the length, width and height of 120cm x 120cm, then inoculating 1 pair of adult wide-band excellent aphids, collecting egg grains every day when the wide-band excellent aphids lay eggs, and rearing at the temperature of 26 +/-1 ℃;
(4) hatching wide-band excellent aphid fly eggs: after wide-band excellent aphid flies lay eggs, taking the wide-band excellent aphid fly eggs out of an insect breeding box filled with filter paper, and placing the wide-band excellent aphid fly eggs into an artificial climate box for hatching, wherein the breeding temperature is 26 +/-1 ℃;
(5) 1-year-old wide-band excellent aphid fly breeding: when wide-band superior aphid fly eggs begin to hatch, feeding myzus persicae in a culture dish according to a ratio of the wide-band superior aphid fly to the myzus persicae of 1:100, and adding the myzus persicae every day, wherein the feeding temperature is 26 +/-1 ℃;
(6) feeding wide-band excellent aphid flies of 2-3 ages: after the wide-band superior aphid flies grow to 2 years old, taking out the rice moth larvae, putting the rice moth larvae into a wide-band superior aphid fly cultivation box according to the standard that 12 heads of each head of the superior aphid flies are fed, and replacing the rice moth larvae every day, wherein the feeding temperature is 26 +/-1 ℃;
(9) collecting and eclosion of broad band superior aphid fly pupae: and after the wide-band superior aphid flies pupate, taking out the wide-band superior aphid fly pupae, placing the wide-band superior aphid fly pupae into an insect raising cage with the length, width and height of 45cm x 45cm, then placing the cage into an artificial climate chamber, and waiting for the wide-band superior aphid flies to eclose.
The number of samples in this example is 300, and 3 times of repetition are performed, and the pupation rate is 91%, the pupation rate is 100%, and the survival rate is 91% for each 100 repeated samples.
Comparative example 1
(1) And (3) myzus persicae breeding: breeding myzus persicae by using tobacco seedlings;
(2) wild collection of wide-band optimized aphids: collecting adult wide-band superior aphid flies in the field by using an insect catching net, and feeding the adult wide-band superior aphid flies indoors in an insect raising cage with the length, width and height of 120cm x 120cm by using 10% of honey water and rape pollen at the feeding temperature of 26 +/-1 ℃;
(3) broad band optimal aphid fly oviposition pairing: putting a tobacco plant with more than 3000 aphids in an insect rearing cage with the length, width and height of 120cm x 120cm, then inoculating 1 pair of adult wide-band excellent aphids, collecting egg grains every day when the wide-band excellent aphids lay eggs, and rearing at the temperature of 26 +/-1 ℃;
(4) hatching wide-band excellent aphid fly eggs: after wide-band excellent aphid flies lay eggs, taking the wide-band excellent aphid fly eggs out of an insect breeding box filled with filter paper, and placing the wide-band excellent aphid fly eggs into an artificial climate box for hatching, wherein the breeding temperature is 26 +/-1 ℃;
(5) 1-year-old wide-band excellent aphid fly breeding: when wide-band superior aphid fly eggs begin to hatch, feeding myzus persicae in a culture dish according to the ratio of the wide-band superior aphid flies to the myzus persicae of 1:150, and adding the myzus persicae every day, wherein the feeding temperature is 26 +/-1 ℃;
(6) feeding wide-band excellent aphid flies of 2-3 ages: after the wide-band superior aphid flies grow to 2 years old, taking out the rice moth larvae, putting the rice moth larvae into a wide-band superior aphid fly culture box for feeding the wide-band superior aphid flies, and replacing the rice moth larvae every day at the feeding temperature of 26 +/-1 ℃;
(10) collecting and eclosion of broad band superior aphid fly pupae: and after the wide-band superior aphid flies pupate, taking out the wide-band superior aphid fly pupae, placing the wide-band superior aphid fly pupae into an insect breeding cage with the length, width and height of 30cm x 30cm-60cm x 60cm, then placing the cage into an artificial climate chamber, and waiting for the wide-band superior aphid flies to eclose.
The number of samples in this example is 300, and 3 times of repetition are performed, and the pupation rate is 94%, the pupation rate is 100%, and the survival rate is 94% when the number of samples per repetition is 100.
The aphid breeding is labor-consuming and time-consuming, the labor cost is high, and the difficulty in regulating and controlling the temperature and humidity in the large-scale aphid breeding process is high, so that the aphid is easy to parasitize; compared with aphid feeding, the rice moth larva feeding technology is simple, the feeding cost is low, and large-scale feeding can be realized in any area and laboratory.
The method for breeding wide-band optimized aphid fly provided by the invention is described in detail, the principle and the implementation mode of the invention are explained by applying specific examples, and the description of the examples is only used for helping to understand the method and the core idea of the invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, there may be variations in the specific embodiments and the application scope, and in summary, the content of the present specification should not be construed as a limitation to the present invention.
Claims (8)
1. A broad-band optimal aphid fly breeding method is characterized in that broad-band optimal aphid fly breeding is carried out by taking rice moth larvae as food sources.
2. The wide-band superior aphid fly breeding method according to claim 1, wherein the feeding method of the rice moth larvae comprises the following steps: the rice moth larvae are obtained by breeding the rice moth with the corn meal and the wheat bran in a weight ratio of 1:1.5-1:2.5, wherein the breeding temperature is 26 +/-1 ℃.
3. The wide-band superior aphid fly breeding method according to claim 2, wherein 2-3-year-old wide-band superior aphid flies are bred with rice moth larvae as a feeding source.
4. The method for breeding the wide-band superior aphid flies according to claim 3, wherein the method for breeding the wide-band superior aphid flies comprises the following steps:
(1) breeding rice moth larvae;
(2) wild collection and breeding of wide-band excellent aphids: collecting wild adult wide-band myzus persicae, putting into an insect rearing cage I, and feeding with 10% honey water and rape pollen;
(3) broad band optimal aphid fly oviposition pairing: placing the tobacco plant with the myzus persicae in an insect rearing cage II, then inoculating 1 pair of adult wide-band superior myzus persicae, and collecting egg granules every day when the wide-band superior myzus persicae lay eggs;
(4) hatching wide-band excellent aphid fly eggs: after the wide-band superior aphid flies lay eggs, taking out wide-band superior aphid fly eggs, placing the wide-band superior aphid fly eggs into an insect breeding box padded with filter paper, and placing the wide-band superior aphid fly eggs into an artificial climate box for hatching;
(5) 1-year-old wide-band excellent aphid fly breeding: when wide-band superior aphid fly eggs begin to hatch, putting myzus persicae into a culture dish for feeding, and adding myzus persicae every day;
(6) feeding wide-band excellent aphid flies of 2-3 ages: after the wide-band superior aphid flies grow to 2 years old, throwing the rice moth larvae into a wide-band superior aphid fly culture box according to the standard that 8-12 heads of each head of wide-band superior aphid fly are fed by the wide-band superior aphid fly, and replacing the rice moth larvae every day;
(7) collecting and eclosion of broad band superior aphid fly pupae: and after the broad-band superior aphid flies pupate, taking out the broad-band superior aphid fly pupae, putting the broad-band superior aphid fly pupae into an insect breeding cage III, then putting the broad-band superior aphid fly pupae into an artificial climate chamber, and waiting for the broad-band superior aphid flies to eclosion.
5. The method for breeding wide-band excellent aphid flies according to claim 4, wherein the feeding temperature of the wide-band excellent aphid flies is 25-27 ℃.
6. The method for breeding the wide-band myzus persicae as claimed in claim 4, wherein the length, width and height of the first insect-raising cage and the second insect-raising cage are both 120cm by 120 cm; the length, width and height of the insect cage III are 30cm x 30cm-60cm x 60 cm.
7. The method for breeding the wide-band superior aphid fly according to claim 4, wherein the number of the individual aphids of the tobacco plant with the myzus persicae in the step (2) is more than 3000.
8. The wide-band superior aphid fly breeding method according to claim 4, wherein in the step (4), myzus persicae is added into the culture dish according to the ratio of wide-band superior aphid fly to myzus persicae of 1:100-1: 150.
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| CN115349581A (en) * | 2022-09-08 | 2022-11-18 | 湖南农业大学 | Breeding substrate and artificial breeding method for myiasis palmaris |
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| 董坤,董艳,罗佑珍: "温度对黑带食蚜蝇生长发育的影响" * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115349581A (en) * | 2022-09-08 | 2022-11-18 | 湖南农业大学 | Breeding substrate and artificial breeding method for myiasis palmaris |
| CN115349581B (en) * | 2022-09-08 | 2024-01-09 | 湖南农业大学 | Feeding substrate and artificial feeding method for aphis palmaris |
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| CN112471082B (en) | 2023-04-25 |
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