CN110235860A - The large-scale breeding method of Syrkhus corollae - Google Patents

The large-scale breeding method of Syrkhus corollae Download PDF

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CN110235860A
CN110235860A CN201910510513.XA CN201910510513A CN110235860A CN 110235860 A CN110235860 A CN 110235860A CN 201910510513 A CN201910510513 A CN 201910510513A CN 110235860 A CN110235860 A CN 110235860A
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corollae
syrkhus
aphid
breeding method
scale breeding
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CN110235860B (en
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李慧
吴孔明
罗淑萍
孙玉凤
贾慧茹
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Institute of Plant Protection of Chinese Academy of Agricultural Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates

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  • Animal Behavior & Ethology (AREA)
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Abstract

The invention belongs to natural enemy insect large-scale breeding fields, and in particular to the large-scale breeding method of Syrkhus corollae.The present invention feeds Syrkhus corollae different larval instar larva using different types of aphid, and is collected to wasp fly pupa, and packaging is sold, to achieve the purpose that Syrkhus corollae the factorial production.Invention achieves easy, efficient feeding objectives, realize the demand of natural enemy insect mass production, and the application for Syrkhus corollae in biological control provides a large amount of worm sources demands.

Description

The large-scale breeding method of Syrkhus corollae
Technical field
The invention belongs to natural enemy insect large-scale breeding field, and in particular to the large-scale breeding side of Syrkhus corollae Method.
Background technique
Syrkhus corollae (Metasyrphus corollae (Fabricius)) belongs to Diptera (Diptera) Hover fly (Syrphidae) insect is China's important resource insect and natural enemy insect.Syrkhus corollae larva is insect predator, is The important predator of the Homoptera insects such as aphid, aleyrodid.Studies have shown that Syrkhus corollae larval phase, preys on cotten aphid 840- 1500, soybean aphid 1500-2000 head is preyed on, 3 age Syrkhus corollae larvas are to the average daily predatory number of brevicoryne brassicae, black peach aphid 217,167 illustrate that Syrkhus corollae is stronger to the control potentiality of phytophthira pest.Meanwhile Syrkhus corollae adult is also The important insect pollinator of nature, research find that Syrkhus corollae is the advantage pollinator of parsley of reserving seed for planting, the kind of wasp fly pollination Sub- increase yield significantly, in addition, Syrkhus corollae pollination is remarkably improved the percentage of fertile fruit of medicinal plant Chinese tamarisk, therefore as pest natural enemy The foundation of insect and insect pollinator, Syrkhus corollae large-scale breeding technology has important economic significance.
In recent years, the prior art studies the growth and development of wasp fly and artificial breeding, and has obtained laboratory The method of interior raising Syrkhus corollae, and a kind of method for only having artificial mass production wasp fly at present can be used for wasp fly factory Change raising, which is sold using wasp fly adult as product.But in production application, using wasp fly adult as product shape State sell there are various disadvantages, if the survival of wasp fly adult is influenced vulnerable to the external world, is only suitable for the transportation range of half a day, if fortune The defeated time is longer, even if supplementing the nutrients, the survival rate of adult can also be seriously affected;When winter ambient temperature is lower, transportational process The survival rate of middle wasp fly is almost nil;Production cost can be dramatically increased if using customization insulating box transport adult.
Each soil individual development of Syrkhus corollae has different characteristics, no matter with the big ash food aphid of which stage of development Fly is to sell form, there are problems.For example, adult often lays eggs to blade, chorion is relatively thin, and the subtle change of environment will Influence its normal incubation;Wasp fly larva is per a large amount of aphid of feeding day by day, and wasp fly larva often rolls up in blade or pierces soil It inside pupates, causes to be difficult to collect wasp fly pupa.
In addition, there are still other difficult points for the extensive raising of Syrkhus corollae, if egg laying amount is low, indoor mass rearing eats aphid When fly, the egg laying amount of single head adult is about 80, far below 600 egg laying amounts of field wild-type adults;Wasp fly larva is certainly Mutually cannibalisation phenomenon is extremely serious, and up to 50% or more, degree increases with larva quantity to be aggravated larval mortality, is such as intended to reduce food aphid Fly larvae is killed each other, then needs to supplement a large amount of aphid, when addition carries aphid plant, the superposition of plant is often resulted in compared with high humidity Degree, seriously affects wasp fly larvae pupation, percentage of pupation is caused to significantly reduce.
Summary of the invention
The purpose of the present invention is to provide a kind of methods of Syrkhus corollae large-scale breeding.
The large-scale breeding method of the Syrkhus corollae of specific embodiment according to the present invention comprising following steps:
(1) appropriate Syrkhus corollae pupa is transferred in dependent insect cage, places pollen mixture and hydromel in dependent insect cage, supplies Feeding mates after pupa is sprouted wings;
(2) the Syrkhus corollae adult to mate in step (1) is transferred in pairs in new dependent insect cage, supply mixing Pollen and hydromel;By in the semen viciae fabae Miao Fangzhi dependent insect cage for carrying aphid, lay eggs for the Syrkhus corollae female adult of post-coitum;
(3) the semen viciae fabae seedling for the carrying Syrkhus corollae ovum that step (2) obtains is transferred on feeding worm frame, until Syrkhus corollae Egg hatching is larva, and 1 age Syrkhus corollae larva on semen viciae fabae seedling is transferred in insect box, adds aphid into insect box daily Worm, until larvae development is to pupating;
(4) Syrkhus corollae pupa obtained by collection step (3), and be put into packing box.
The large-scale breeding method of the Syrkhus corollae of specific embodiment according to the present invention, pollen mixture are that weight ratio is The rape pollen of 3:1: zasiokaurin.
The large-scale breeding method of the Syrkhus corollae of specific embodiment according to the present invention, the hydromel are that concentration is 10% acacia honey.
The large-scale breeding method of the Syrkhus corollae of specific embodiment according to the present invention, in step (1), (2), big ash The condition of culture of wasp fly be temperature be 25 ± 1 DEG C, light application time L:D=16:8, intensity of illumination 22000Lux, humidity are 20%-50%.
The large-scale breeding method of the Syrkhus corollae of specific embodiment according to the present invention, in step (2), on semen viciae fabae seedling The aphid of carrying is aphis craccivora.
The large-scale breeding method of the Syrkhus corollae of specific embodiment according to the present invention in step (3), shifts larva When, shake fills the dew hole plastic crate of semen viciae fabae seedling, falls to 1 age Syrkhus corollae larva in insect box, while adding aphid When feeding wasp fly larva, also fed using the method.
The large-scale breeding method of the Syrkhus corollae of specific embodiment according to the present invention, in step (3), the aphid of addition Worm includes pea tail reparing aphid and aphis craccivora.
The large-scale breeding method of the Syrkhus corollae of specific embodiment according to the present invention, in step (3), big ash food aphid The rearing conditions of fly larvae are that temperature is 25 ± 1 DEG C, and intensity of illumination is natural light, humidity 20%-50%.
The large-scale breeding method of the Syrkhus corollae of specific embodiment according to the present invention, in step (3), insect box Top surface is equipped with opening, is covered with gauze in the opening, sand grains is painted on the inner wall of the insect box, the mesh number of sand grains is 120 mesh.
The large-scale breeding method of the Syrkhus corollae of specific embodiment according to the present invention, in step (4), in packing box Bottom be laid with two layers of filter paper, offer air hole on the top surface of the packing box;Syrkhus corollae pupa is placed on the filter paper On, fresh semen viciae fabae seedling is layered on to the top of Syrkhus corollae pupa.
Beneficial effects of the present invention:
Present invention discover that pupa is the most conformal state that Syrkhus corollae is sold as product, can not only meet up to 6 days Haulage time, and do not influenced vulnerable to extraneous low temperature or high temperature, packing cost is cheap, in addition, Syrkhus corollae adult institute's postpartum Pupa turns into female adult in generation, the ratio of male worm is 1-2, and female-male proportion is stablized, and sells pupa as product, and male and female proportion can also expire Sufficient field greenhouse pest pest control effect.
The present invention provides a kind of large-scale breeding method of Syrkhus corollae, solves practical the factorial production and sells food aphid Each difficult point during fly, realizes the demand of natural enemy insect mass production, has reached easy, has efficiently raised mesh Mark realizes to utilize a large amount of worm sources demands of the phytophthira pest on Syrkhus corollae biological control crops and fruits and vegetables.
Specific embodiment
Syrkhus corollae ontogeny is divided into ovum, larva, 4 worm state phases of pupa and adult, wherein larval stage can be divided into again 1 instar larvae, 2 instar larvaes and 3 instar larvaes.Larval phase Syrkhus corollae is insect predator, main all kinds of aphids of feeding;Adult stage For phytophagous, feeding pollen and nectar are to continue the service life.
Embodiment 1
1. kind of plant host plant
Semen viciae fabae seedling is planted in the stronger glasshouse room of illumination in factory.It plants specification and process is as follows:
Semen viciae fabae is planted using nutritive cube (24cm × 5cm × 12cm), matrix is small vermiculite, plantation 24 in each nutritive cube Broad Bean Seeds uniformly sprinkle the small vermiculite of thickness about 0.5cm in the surface of the seed.
Nutritive cube is placed in feeding seedling box (50cm × 35cm × 15cm), the watering of Xiang Yangmiao cassette bottom portion, Broad Bean Seeds hair Bud when height of seedling about 2cm, is rested seedling box and is transferred in aphid receptacle and carry out inoculation aphid.
It is not necessarily to supplementary light in greenhouse, keeps room temperature, humidity no requirement (NR).
2. raising variety classes aphid
Pea tail reparing aphid worm, clover aphid are raised respectively in the stronger glass insectary of natural light.
Aphis craccivora greenhouse: temperature: 25 DEG C, humidity: 30%-60%, L:D=16:8, luminous intensity 5000Lux, semen viciae fabae height of seedling When about 5cm-8cm, shearing carries the semen viciae fabae seedling of a large amount of aphids, is laid on semen viciae fabae seedling, Aphed population on seedling is observed, when germination On semen viciae fabae seedling after uniform fold aphid, the semen viciae fabae seedling laid flat is removed.
Pea tail reparing aphid greenhouse: temperature: 19 DEG C, humidity: 30%-60%, L:D=16:8, luminous intensity 5000Lux, semen viciae fabae When height of seedling about 3cm-5cm, the inoculation of aphid is carried out.
3. being inoculated with Syrkhus corollae
The a large amount of Syrkhus corollae pupas of (55cm × 33cm × 40cm, 120 mesh) transfer into dependent insect cage, 100-200, on pupa Side covers appropriate semen viciae fabae seedling leaf, to its emergence.The culture dish that 2 bottoms are covered with pollen mixture is placed in cage, 2 soak bee The culture dish of mulse absorbent cotton, for the imago feeding come out of sprouting wings.
4. breeding wasp fly inoculation
It takes the 10 pairs of wasp fly adults to mate in dependent insect cage to be transferred in new dependent insect cage, mixing is placed in dependent insect cage Pollen and the absorbent cotton for soaking hydromel, the interior nutritive cube for placing about 10cm 4 high of dependent insect cage, interior place of nutritive cube carry clover The semen viciae fabae seedling of aphid is for wasp fly oviposition, and replacement carries the semen viciae fabae seedling of aphis craccivora daily.
Wasp fly lays eggs after mating 3 days, observes wasp fly daily and breeds situation, 10 pairs of wasp flys are every in breeding period dependent insect cage It produces about 10 ovum on every plant of semen viciae fabae seedling, and the semen viciae fabae seedling for carrying wasp fly ovum is taken out, is transferred on feeding worm frame and waits ovum Hatching, while shifting and continuing to lay eggs for wasp fly in new semen viciae fabae seedling to dependent insect cage, it is recycled with this.
Wasp fly adult copulation chamber, transfer room condition: temperature: 25 ± 1 DEG C, humidity: 20%-50%, illumination: 16L:8D, Intensity of illumination is 22000Lux.
5. raising wasp fly larva
Insect box (44cm × 30cm × 15cm, 30cm × 20cm opening above box cover 120 mesh gauzes in opening), outside box Yellow cardboard is pasted in all bottoms, smears finely ground particle substance to four wall of box daily, guarantees that aphid stays in insect box bottom for wasp fly Feeding.Larva raising room condition: temperature: 25 ± 1 DEG C;Humidity: 20%-50%.Intensity of illumination is natural light, without supplement Illumination.
The hatching situation for supporting wasp fly ovum on worm frame semen viciae fabae seedling is observed, after about 3 days, egg hatching is 1 instar larvae, and semen viciae fabae seedling When upper Aphed population is substantially reduced, shearing semen viciae fabae seedling is transferred to bottom and four walls dew hole, and hole size is for aphid and wasp fly It in the plastic crate that larva passes through, closes the lid, shakes plastic crate, wasp fly larva and aphid is made to fall to insect box bottom, The raising of wasp fly larva is carried out, double pump fills the modeling for carrying pea tail reparing aphid and aphis craccivora semen viciae fabae seedling stalk daily morning and evening Charging basket falls to aphid in insect box, for wasp fly larval feeding.
In addition, daily using small brushes in insect box four walls smear 120 mesh finely ground particle substances, prevent aphid swash and and When aphid is added into box, after about 4 days, Syrkhus corollae larva entered for 3 latter stages in age, and Syrkhus corollae larva body is long to be shortened, back Portion's decorative pattern is reduced, and body colour bleaches, pupates into pupa time.
6. collecting wasp fly
Wasp fly larvae pupation situation in daily observation insect box gently will with tweezers daily after larva enters pupa time The wasp fly to pupate on the same day is chosen, and is transferred in wasp fly pupa packing box, clears up aphid in insect box in time and sloughs off, to wasp fly children After worm all pupates, the new first raising for incubating progress next group wasp fly larva in wasp fly larva to insect box is shifted.
7. packing wasp fly
Two layers of filter paper or gauze are spread in the bottom paper package box (13cm × 8cm × 9cm), tile wasp fly pupa on filter paper About 200, top covers multilayer semen viciae fabae seedling leaf and keeps humidity, uses gauze (120 at packing box top drilling (6cm × 6cm) Mesh) sealing.
8. the utilization of experimental material
The utilization of semen viciae fabae seedling: Broad Bean Leaves after shaking off aphid to insect box, can be covered on by feeding wasp fly larva On wasp fly pupa, the packaging of wasp fly product is carried out.
The recycling of matrix: after the shearing of semen viciae fabae seedling, vermiculite small in nutritive cube can be poured out, is crushed, after high-temperature sterilization It can reuse.
Embodiment 2 investigates influence of each cultivation parameter to Syrkhus corollae
2.1 investigating the influence for carrying the plant pair Syrkhus corollae reproductive capacity of aphid group in daily replacement dependent insect cage
In the mating, oviposition and 1 instar larvae stage of wasp fly, investigates replacement carrying aphid group's semen viciae fabae seedling and wasp fly is bred Influence, setting experimental group and two processing groups of control group, experimental group is that daily replacement carries aphid group's semen viciae fabae seedling, and control group is not Semen viciae fabae seedling is replaced, the results are shown in Table 1:
The replacement daily of table 1 carries the influence of aphid plant pair Syrkhus corollae development
The results are shown in Table 1, and total egg laying amount of experimental group single head wasp fly is 604.4, and egg hatching rate reaches 90.1%, Because the oviposition of wasp fly can be stimulated in the mating stage replacement plant of wasp fly, the oviposition of single head wasp fly is significantly improved Amount, and ensure that wasp fly instar larvae is consistent on homophyletic plant in larval stage replacement plant, avoid high instar larvae to ovum and The feeding of low instar larvae significantly reduces degree of killing each other.
Influence of 2.2 the feeding Aphid Species, age to wasp fly larva
Three processing groups are set, the semen viciae fabae seedling for carrying different Aphid Species, wasp fly are provided it in wasp fly breeding period It lays eggs on semen viciae fabae seedling, larva is retransferred after egg hatching development is 1 instar larvae and carries out subsequent raising, control group 1 is to carry pea Beans repair the semen viciae fabae seedling of tail aphid, and control group 2 is the semen viciae fabae seedling for carrying pea tail reparing aphid and aphis craccivora mixed population, and experimental group is to carry The semen viciae fabae seedling of aphis craccivora.Experimental result is as shown in table 2:
The influence that 2 Aphid Species of table breed wasp fly
The results are shown in Table 2, and wasp fly egg laying amount and egg hatching rate are higher in aphis craccivora group.Wasp fly lays eggs mechanism by more Kind factor influences, and wherein it is fairly obvious to breed influence on it for Aphid Species, quantity.In daily breeding process, pea tail reparing aphid with For aphis craccivora when mixing raising on semen viciae fabae seedling, pea tail reparing aphid often shows apparent competitive advantage, and quantity is much higher than aphis craccivora.
The influence that Syrkhus corollae quantity breeds it is launched in 2.3 dependent insect cages
The wasp fly adult that 5 pairs, 10 pairs, 20 pairs different numbers are shifted into dependent insect cage, records single head wasp fly egg laying amount. The results are shown in Table 3:
The influence that the supply volume of 3 Syrkhus corollae of table breeds it
Studies have shown that since dependent insect cage space and Aphed population limit, when transfer wasp fly adult quantity is more, single head food Aphid fly reproductive capacity reduces, and daily transfer semen viciae fabae seedling inconvenience operation, wasp fly adult can flee from dependent insect cage;Wasp fly adult quantity When less, if single head wasp fly unusual death, the total egg laying amount of wasp fly on semen viciae fabae seedling can be significantly affected in cage.Therefore, exist When placing 10 pairs of wasp fly adults in cage, the egg laying amount of single head wasp fly adult is higher.
The influence that 2.4 insect box methods feeding wasp fly larva develops it
It is investigated in the semen viciae fabae seedling to insect box for shaking off aphid on semen viciae fabae seedling, shearing carrying aphid daily respectively and feeds wasp fly Larva records survival rate of larvae, hundred pupa weights, as a result as indicated at 4:
The influence that 4 aphid addition manner of table develops Syrkhus corollae
The results are shown in Table 4, shakes off on semen viciae fabae seedling in aphid to insect box daily, and hundred pupa weights are shaken off up to 3.01g The addition manner of aphid is obviously improved survival rate of larvae, while guaranteeing that wasp fly larva often daily can obtain enough nutrition in box, Rate of development is consistent, improves wasp fly larva, pupa and adult quality.
2.5 investigate influence of the haulage time to form pupa is sold
During wasp fly raising, selects at random 5 months, 1 insect box is randomly selected in every month, totally 5 insect box, After wasp fly pupates in insect box, pupa is transferred in dependent insect cage, after pupa emergence, records wasp fly adult male and female quantity, And ratio is calculated, the results are shown in Table 5:
Female-male proportion after 5 wasp fly pupa of table is sprouted wings
The result shows that the form of wasp fly pupa is more convenient for transporting compared to adult, and it can avoid adult during transportation Death, and transportation cost is lower, furthermore wasp fly pupa is hatched as female, male imago ratio in a certain range, has no significance difference It is different.
In wasp fly pupa transportational process, packing box uses different fillings, and sprouting wings on wasp fly pupa, it is different to influence, as a result such as Shown in table 6:
6 different fillings of table, which sprout wings on wasp fly pupa, to be influenced
The experimental results showed that using semen viciae fabae seedling as filler when, the normal eclosion rate highest of wasp fly pupa.
To sum up, raising each stage technique parameter of Syrkhus corollae is improved, as shown in the table:
7 wasp fly of table raises each stage technique parameter improvement and compares
Single pair wasp fly adult obtains offspring's wasp fly adult quantity and significantly improves after improvement, and correlated results is as follows:

Claims (10)

1. the large-scale breeding method of Syrkhus corollae, which is characterized in that the described method comprises the following steps:
(1) Syrkhus corollae pupa is transferred in dependent insect cage, places pollen mixture and hydromel in dependent insect cage, eats aphid for big ash Feeding mates after fly pupa is sprouted wings;
(2) the Syrkhus corollae adult to mate in step (1) is transferred in pairs in new dependent insect cage, supplies pollen mixture And hydromel;By in the semen viciae fabae Miao Fangzhi dependent insect cage for carrying aphid, and semen viciae fabae seedling is replaced daily, for the Syrkhus corollae of post-coitum Female adult oviposition;
(3) the semen viciae fabae seedling for the carrying Syrkhus corollae ovum that step (2) obtains is transferred on feeding worm frame, Syrkhus corollae egg hatching After 1 instar larvae, 1 instar larvae on semen viciae fabae seedling is transferred in insect box, adds aphid into insect box daily, until larva It is developed to and pupates;
(4) Syrkhus corollae pupa obtained by collection step (3), and be put into packing box.
2. the large-scale breeding method of Syrkhus corollae according to claim 1, which is characterized in that pollen mixture is weight Than the rape pollen for 3:1: zasiokaurin.
3. the large-scale breeding method of Syrkhus corollae according to claim 1, which is characterized in that the hydromel is dense The acacia honey that degree is 10%.
4. the large-scale breeding method of Syrkhus corollae according to claim 1, which is characterized in that in step (1), (2), Condition of culture be temperature be 25 ± 1 DEG C, light application time L:D=16:8, intensity of illumination 22000Lux, humidity 20%- 50%.
5. the large-scale breeding method of Syrkhus corollae according to claim 1, which is characterized in that in step (2), semen viciae fabae The aphid carried on seedling is aphis craccivora.
6. the large-scale breeding method of Syrkhus corollae according to claim 1, which is characterized in that in step (3), transfer When larva, 1 instar larvae on semen viciae fabae seedling is shaken off to insect box.
7. the large-scale breeding method of Syrkhus corollae according to claim 1, which is characterized in that in step (3), addition Aphid include pea tail reparing aphid and aphis craccivora.
8. the large-scale breeding method of Syrkhus corollae according to claim 1, which is characterized in that in step (3), big ash The rearing conditions of wasp fly larva are that temperature is 25 ± 1 DEG C, and intensity of illumination is natural light, humidity 20%-50%.
9. the large-scale breeding method of Syrkhus corollae according to claim 1, which is characterized in that in step (3), support worm The top surface of box is equipped with opening, is covered with gauze in the opening, sand grains, the mesh of sand grains are painted on the inner wall of the insect box Number is 120 mesh.
10. the large-scale breeding method of Syrkhus corollae according to claim 1, which is characterized in that in step (4), packet Bottom in mounted box is laid with two layers of filter paper, offers air hole on the top surface of the packing box;Syrkhus corollae pupa is placed on institute It states on filter paper, fresh semen viciae fabae seedling is layered on to the top of Syrkhus corollae pupa.
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CN112471082A (en) * 2020-12-17 2021-03-12 湖南省烟草公司长沙市公司 Broad-band optimized aphid fly breeding method
CN112602860A (en) * 2020-12-17 2021-04-06 湖南省烟草公司长沙市公司 Artificial feed for feeding wide-band excellent aphids and preparation method and application thereof
CN112471082B (en) * 2020-12-17 2023-04-25 湖南省烟草公司长沙市公司 Broadband preferred aphid breeding method
CN112602860B (en) * 2020-12-17 2023-10-20 湖南省烟草公司长沙市公司 Artificial feed for feeding broadband eudipleus aphis, and preparation method and application thereof
CN113973784A (en) * 2021-12-09 2022-01-28 河南农业大学 Method for preventing insects, ant lions, from killing each other by artificial feeding and application thereof
CN115349580A (en) * 2022-09-08 2022-11-18 湖南农业大学 Breeding substrate and artificial breeding method for cyenopsis griseus
CN115349580B (en) * 2022-09-08 2024-01-09 湖南农业大学 Feeding substrate and artificial feeding method for aphids with tube

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