CN112602860A - Artificial feed for feeding wide-band excellent aphids and preparation method and application thereof - Google Patents
Artificial feed for feeding wide-band excellent aphids and preparation method and application thereof Download PDFInfo
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- CN112602860A CN112602860A CN202011495704.2A CN202011495704A CN112602860A CN 112602860 A CN112602860 A CN 112602860A CN 202011495704 A CN202011495704 A CN 202011495704A CN 112602860 A CN112602860 A CN 112602860A
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- 241001124076 Aphididae Species 0.000 title claims abstract description 111
- 238000002360 preparation method Methods 0.000 title claims abstract description 6
- 241001142294 Chamaemyiidae Species 0.000 claims abstract description 81
- 238000009395 breeding Methods 0.000 claims abstract description 38
- 230000001488 breeding effect Effects 0.000 claims abstract description 38
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 23
- 238000000034 method Methods 0.000 claims abstract description 17
- 229920001817 Agar Polymers 0.000 claims abstract description 14
- 241000382353 Pupa Species 0.000 claims abstract description 14
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 14
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims abstract description 14
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 14
- 229930006000 Sucrose Natural products 0.000 claims abstract description 14
- 239000008272 agar Substances 0.000 claims abstract description 14
- 229960004793 sucrose Drugs 0.000 claims abstract description 14
- 235000013601 eggs Nutrition 0.000 claims description 52
- 241000238631 Hexapoda Species 0.000 claims description 44
- 241000721621 Myzus persicae Species 0.000 claims description 38
- 230000003203 everyday effect Effects 0.000 claims description 18
- 230000032669 eclosion Effects 0.000 claims description 13
- 230000012447 hatching Effects 0.000 claims description 13
- 230000000384 rearing effect Effects 0.000 claims description 12
- 239000000843 powder Substances 0.000 claims description 10
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims description 9
- 244000061176 Nicotiana tabacum Species 0.000 claims description 8
- 235000012907 honey Nutrition 0.000 claims description 8
- 230000037237 body shape Effects 0.000 claims description 6
- 230000017448 oviposition Effects 0.000 claims description 6
- 235000013305 food Nutrition 0.000 claims description 5
- 239000005720 sucrose Substances 0.000 claims description 3
- 238000009835 boiling Methods 0.000 claims description 2
- 239000008187 granular material Substances 0.000 claims description 2
- 238000005303 weighing Methods 0.000 claims description 2
- 241001305034 Aphidius gifuensis Species 0.000 claims 1
- 241000255925 Diptera Species 0.000 claims 1
- 230000019617 pupation Effects 0.000 abstract description 12
- 230000007774 longterm Effects 0.000 abstract description 2
- 230000018109 developmental process Effects 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 2
- 241000208125 Nicotiana Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
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- Sustainable Development (AREA)
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- Microbiology (AREA)
- Animal Behavior & Ethology (AREA)
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- Feed For Specific Animals (AREA)
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- Fodder In General (AREA)
Abstract
The invention discloses an artificial feed for feeding wide-band superior aphid flies, a preparation method and application thereof, wherein the artificial feed comprises the following components in parts by weight: 25-35 parts of bee pupa; agar 0.5-1 min; 4-6 parts of beer yeast, 4-6 parts of cane sugar and 55-65 parts of water. The wide-band excellent-feeding aphid fly artificial feed provided by the invention can realize the replacement of wide-band excellent-feeding aphid fly foodstuff, can reduce the artificial scale breeding cost of wide-band excellent-feeding aphid flies, and can reach 72% of pupation rate and 94.4% of pupation rate in the long-term feeding process.
Description
Technical Field
The invention relates to the field of artificial scale breeding of natural enemy insects, in particular to an artificial feed for breeding wide-band superior aphid flies.
Background
Predatory natural enemy insects are widely applied to biological control of agricultural and forestry pests, and play a great role in protecting the yield and quality safety of agricultural and forestry products and protecting the environment from pesticide pollution.
The wide-band excellent aphid fly can control aphids on various agricultural and forestry crops, and is a good predatory natural enemy insect.
At present, the artificial breeding of the broad band superior aphid flies is mainly to feed the aphids, but the breeding of the aphids is labor-consuming and time-consuming, the labor cost is high, and the temperature and humidity regulation and control difficulty in the large-scale breeding process of the aphids is high, so that the aphids are easily parasitized.
Compared with aphid feeding, the artificial feed feeding technology is simple, the feeding cost is low, large-scale feeding can be realized in any area and laboratory, the pupation rate can reach 72%, and the pupation rate can reach 94.4%.
Disclosure of Invention
In view of the problems, the invention provides the artificial feed more suitable for artificial large-scale feeding of the wide-band superior aphid flies and the method for feeding the wide-band superior aphid flies by using the artificial feed.
In order to achieve the purpose, the invention adopts the following technical scheme:
an artificial feed for feeding wide-band superior aphid flies comprises the following components in parts by weight: 25-35 parts of bee pupa; agar 0.5-1 min; 4-6 parts of beer yeast, 4-6 parts of cane sugar and 55-65 parts of water.
Preferably, the composition comprises the following components in parts by weight: 30 parts of bee pupa powder, 0.5 part of agar, 5 parts of beer yeast, 4.5 parts of cane sugar and 60 parts of water.
Adopt above-mentioned technical scheme's beneficial effect: the bee pupa powder and the beer yeast provide nutrient substances required by development for the broad-band superior aphid fly, the sucrose provides energy for the broad-band superior aphid fly and stimulates the broad-band superior aphid fly to take food, and the agar and the water ensure that the artificial feed is semisolid, so that the broad-band superior aphid fly can conveniently take food.
The preparation method of the artificial feed for feeding wide-band superior aphid flies comprises the following steps:
(1) weighing the required bee pupa powder, agar, beer yeast, cane sugar and water according to the weight parts;
(2) mixing bee pupa powder, beer yeast and sucrose into half volume of 25-30 deg.C water to obtain solution A; dissolving agar in the other half volume of boiling water to obtain solution B;
(3) after the solution B was cooled to 60 ℃, A, B solution was mixed and left to stand until the artificial feed became semisolid.
The invention also discloses a method for feeding wide-band superior aphid flies by taking the artificial feed as a food source.
Preferably, the 3-year-old broad-band excellent aphid flies are fed by taking the artificial feed as a food source.
Because the death rate of the wide-band excellent aphid flies of 1-2 ages fed with the artificial feed is higher, the wide-band excellent aphid flies of 1-2 ages are fed with the living body myzus persicae, and the wide-band excellent aphid flies of 3-5 ages are fed with the artificial feed.
Preferably, the method for feeding wide-band myzus persicae comprises the following steps:
(1) wild collection and breeding of wide-band excellent aphids: collecting wild adult wide-band myzus persicae, putting into an insect rearing cage I, and feeding with 10% honey water and rape pollen;
(2) broad band optimal aphid fly oviposition pairing: placing the tobacco plant with the myzus persicae in an insect rearing cage II, then inoculating 1 pair of adult wide-band superior myzus persicae, and collecting egg granules every day when the wide-band superior myzus persicae lay eggs;
(3) hatching wide-band excellent aphid fly eggs: after the wide-band superior aphid flies lay eggs, taking out wide-band superior aphid fly eggs, placing the wide-band superior aphid fly eggs into an insect breeding box padded with filter paper, and placing the wide-band superior aphid fly eggs into an artificial climate box for hatching;
(4) feeding wide-band excellent aphid flies of 1-2 ages: when wide-band excellent aphid fly eggs begin to hatch, putting myzus persicae into the insect breeding box for breeding, and adding myzus persicae every day;
(5) feeding 3-year-old wide-band excellent aphids: after the wide-band superior aphid flies grow to 3 years old, feeding the wide-band superior aphid flies with artificial feed, and replacing the artificial feed every day, wherein the artificial feed is added according to 1.5 times of the body shape of the wide-band superior aphid flies;
(6) collecting and eclosion of broad band superior aphid fly pupae: and after the broad-band superior aphid flies pupate, taking out the broad-band superior aphid fly pupae, putting the broad-band superior aphid fly pupae into an insect breeding cage III, then putting the broad-band superior aphid fly pupae into an artificial climate chamber, and waiting for the broad-band superior aphid flies to eclosion.
Preferably, in the above feeding method, the feeding temperature of the wide-band myzus persicae is 26 ± 1 ℃.
Preferably, the length, width and height of the first insect cage and the second insect cage are both 120cm by 120 cm; the length, width and height of the insect cage III are 30cm x 30cm-60cm x 60 cm.
Preferably, the number of the individual myzus persicae in the tobacco plant with myzus persicae in the step (2) is more than 3000.
Preferably, in the step (4), myzus persicae is thrown into the insect breeding box according to the head ratio of broad band excellent aphid flies to myzus persicae of 1:100-1: 150.
Compared with the prior art, the invention has the beneficial effects that:
the artificial feed has simple preparation method, can replace aphids in the 3-year stage of wide-band excellent aphid fly mass feeding, can reduce the artificial scale breeding cost of wide-band excellent aphid fly, and the pupation rate of wide-band excellent aphid fly can reach 72% and the pupation rate can reach 94.4% in the long-term feeding process.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
(1) Preparing artificial feed: 30 parts of bee pupa powder, 0.5 part of agar, 5 parts of beer yeast, 4.5 parts of cane sugar and 60 parts of water;
(2) wild collection of wide-band optimized aphids: collecting adult wide-band superior aphid flies in the field by using an insect catching net, and feeding the adult wide-band superior aphid flies indoors in an insect raising cage with the length, width and height of 120cm x 120cm by using 10% of honey water and rape pollen at the feeding temperature of 26 +/-1 ℃;
(3) broad band optimal aphid fly oviposition pairing: putting a tobacco plant with more than 3000 aphids in an insect rearing cage with the length, width and height of 120cm x 120cm, then inoculating 1 pair of adult wide-band excellent aphids, collecting egg grains every day when the wide-band excellent aphids lay eggs, and rearing at the temperature of 26 +/-1 ℃;
(4) hatching wide-band excellent aphid fly eggs: after wide-band excellent aphid flies lay eggs, taking out wide-band excellent aphid fly eggs, placing the wide-band excellent aphid fly eggs into an insect breeding box padded with filter paper, and placing the wide-band excellent aphid fly eggs into an artificial climate box for hatching, wherein the breeding temperature is 26 +/-1 ℃;
(5) feeding wide-band excellent aphid flies of 1-2 ages: when wide-band superior aphid fly eggs begin to hatch, feeding myzus persicae in an insect breeding box according to the ratio of the wide-band superior aphid flies to the myzus persicae of 1:150, and adding the myzus persicae every day, wherein the feeding temperature is 26 +/-1 ℃;
(6) feeding 3-year-old wide-band excellent aphids: after the wide-band excellent aphid flies grow to 3 years old, feeding the wide-band excellent aphid flies with artificial feed, feeding the wide-band excellent aphid flies 1.5 times of the body shape of the wide-band excellent aphid flies, and replacing the artificial feed every day, wherein the feeding temperature is 26 +/-1 ℃;
(7) collecting and eclosion of broad band superior aphid fly pupae: and after the wide-band superior aphid flies pupate, taking out the wide-band superior aphid fly pupae, placing the wide-band superior aphid fly pupae into an insect raising cage with the length, width and height of 30cm x 30cm, then placing the cage into an artificial climate chamber, and waiting for the wide-band superior aphid flies to eclose.
The number of samples in this example is 100, the pupation rate is 72%, the pupal eclosion rate is 94.4%, the development history of the larvae is 8.3 + -0.5 days, the development history of the pupae is 8.5 + -0.5 days, the length of the adult is 9.7 + -0.3 cm, and the wingspan is 8.2 + -0.4 cm.
Example 2
(1) Preparing artificial feed: 25 parts of bee pupa powder, 0.5 part of agar, 5.5 parts of beer yeast, 4 parts of cane sugar and 65 parts of water;
(2) wild collection of wide-band optimized aphids: collecting adult wide-band superior aphid flies in the field by using an insect catching net, and feeding the adult wide-band superior aphid flies indoors in an insect raising cage with the length, width and height of 120cm x 120cm by using 10% of honey water and rape pollen at the feeding temperature of 26 +/-1 ℃;
(3) broad band optimal aphid fly oviposition pairing: putting a tobacco plant with more than 3000 aphids in an insect rearing cage with the length, width and height of 120cm x 120cm, then inoculating 1 pair of adult wide-band excellent aphids, collecting egg grains every day when the wide-band excellent aphids lay eggs, and rearing at the temperature of 26 +/-1 ℃;
(4) hatching wide-band excellent aphid fly eggs: after wide-band excellent aphid flies lay eggs, taking out wide-band excellent aphid fly eggs, placing the wide-band excellent aphid fly eggs into an insect breeding box padded with filter paper, and placing the wide-band excellent aphid fly eggs into an artificial climate box for hatching, wherein the breeding temperature is 26 +/-1 ℃;
(5) feeding wide-band excellent aphid flies of 1-2 ages: when wide-band optimal-feeding aphid eggs begin to hatch, feeding the myzus persicae in an insect breeding box according to the ratio of the wide-band optimal-feeding aphid eggs to the myzus persicae of 1:100, and adding the myzus persicae every day, wherein the feeding temperature is 26 +/-1 ℃;
(6) feeding 3-year-old wide-band excellent aphids: after the wide-band excellent aphid flies grow to 3 years old, feeding the wide-band excellent aphid flies with artificial feed, feeding the wide-band excellent aphid flies 1.5 times of the body shape of the wide-band excellent aphid flies, and replacing the artificial feed every day, wherein the feeding temperature is 26 +/-1 ℃;
(8) collecting and eclosion of broad band superior aphid fly pupae: and after the wide-band superior aphid flies pupate, taking out the wide-band superior aphid fly pupae, placing the wide-band superior aphid fly pupae into an insect raising cage with the length, width and height of 45cm x 45cm, then placing the cage into an artificial climate chamber, and waiting for the wide-band superior aphid flies to eclose.
The number of samples in this example is 100, the pupation rate is 70%, the pupal eclosion rate is 90%, the development period of the larva is 8.5 + -0.4 days, the development period of the pupa is 8.8 + -0.7 days, the adult length is 9.8 + -0.4 cm, and the wingspan is 8.3 + -0.5 cm.
Example 3
(1) Preparing artificial feed: 35 parts of bee pupa powder, 1 part of agar, 4 parts of beer yeast, 5 parts of cane sugar and 55 parts of water;
(2) wild collection of wide-band optimized aphids: collecting adult wide-band superior aphid flies in the field by using an insect catching net, and feeding the adult wide-band superior aphid flies indoors in an insect raising cage with the length, width and height of 120cm x 120cm by using 10% of honey water and rape pollen at the feeding temperature of 26 +/-1 ℃;
(3) broad band optimal aphid fly oviposition pairing: putting a tobacco plant with more than 3000 aphids in an insect rearing cage with the length, width and height of 120cm x 120cm, then inoculating 1 pair of adult wide-band excellent aphids, collecting egg grains every day when the wide-band excellent aphids lay eggs, and rearing at the temperature of 26 +/-1 ℃;
(4) hatching wide-band excellent aphid fly eggs: after wide-band excellent aphid flies lay eggs, taking out wide-band excellent aphid fly eggs, placing the wide-band excellent aphid fly eggs into an insect breeding box padded with filter paper, and placing the wide-band excellent aphid fly eggs into an artificial climate box for hatching, wherein the breeding temperature is 26 +/-1 ℃;
(5) feeding wide-band excellent aphid flies of 1-2 ages: when wide-band optimal-feeding aphid eggs begin to hatch, feeding the myzus persicae in an insect breeding box according to the ratio of the wide-band optimal-feeding aphid eggs to the myzus persicae of 1:125, adding the myzus persicae every day, and keeping the feeding temperature at 26 +/-1 ℃;
(6) feeding 3-year-old wide-band excellent aphids: after the wide-band excellent aphid flies grow to 3 years old, feeding the wide-band excellent aphid flies with artificial feed, feeding the wide-band excellent aphid flies 1.5 times of the body shape of the wide-band excellent aphid flies, and replacing the artificial feed every day, wherein the feeding temperature is 26 +/-1 ℃;
(9) collecting and eclosion of broad band superior aphid fly pupae: and after the wide-band superior aphid flies pupate, taking out the wide-band superior aphid fly pupae, placing the wide-band superior aphid fly pupae into an insect raising cage with the length, width and height of 60cm x 60cm, then placing the cage into an artificial climate chamber, and waiting for the wide-band superior aphid flies to eclose.
The number of samples in this example is 100, the pupation rate is 70%, the pupal eclosion rate is 88.6%, the development history of the larvae is 8.6 + -0.4 days, the development history of the pupae is 8.5 + -0.7 days, the length of the adult is 9.5 + -0.4 cm, and the wingspan is 8.1 + -0.5 cm.
Comparative example 1
(1) Preparing artificial feed: 5 parts of cane sugar, 20 parts of honey, 0.5 part of agar, 5 parts of beer yeast and 70 parts of water;
(2) wild collection of wide-band optimized aphids: collecting adult wide-band superior aphid flies in the field by using an insect catching net, and feeding the adult wide-band superior aphid flies indoors in an insect raising cage with the length, width and height of 120cm x 120cm by using 10% of honey water and rape pollen at the feeding temperature of 26 +/-1 ℃;
(3) broad band optimal aphid fly oviposition pairing: putting a tobacco plant with more than 3000 aphids in an insect rearing cage with the length, width and height of 120cm x 120cm, then inoculating 1 pair of adult wide-band excellent aphids, collecting egg grains every day when the wide-band excellent aphids lay eggs, and rearing at the temperature of 26 +/-1 ℃;
(4) hatching wide-band excellent aphid fly eggs: after wide-band excellent aphid flies lay eggs, taking the wide-band excellent aphid fly eggs out of an insect breeding box filled with filter paper, and placing the wide-band excellent aphid fly eggs into an artificial climate box for hatching, wherein the breeding temperature is 26 +/-1 ℃;
(5) feeding wide-band excellent aphid flies of 1-2 ages: when wide-band superior aphid fly eggs begin to hatch, feeding myzus persicae in an insect breeding box according to the ratio of the wide-band superior aphid flies to the myzus persicae of 1:150, and adding the myzus persicae every day, wherein the feeding temperature is 26 +/-1 ℃;
(6) feeding 3-year-old wide-band excellent aphids: after the wide-band excellent aphid flies grow to 3 years old, feeding the wide-band excellent aphid flies with artificial feed, feeding the wide-band excellent aphid flies 1.5 times of the body shape of the wide-band excellent aphid flies, and replacing the artificial feed every day, wherein the feeding temperature is 26 +/-1 ℃;
(7) collecting and eclosion of broad band superior aphid fly pupae: and after the wide-band superior aphid flies pupate, taking out the wide-band superior aphid fly pupae, placing the wide-band superior aphid fly pupae into an insect breeding cage with the length, width and height of 30cm x 30cm-60cm x 60cm, then placing the cage into an artificial climate chamber, and waiting for the wide-band superior aphid flies to eclose.
The number of samples of this comparative example was 100, the pupation rate was 67%, the pupal eclosion rate was 85.1%, the growth history of the larvae was 6.4. + -. 0.4 days, the growth history of the pupae was 9.0. + -. 0.7 days, the length of the adult was 9.1. + -. 0.6cm, and the wingspan was 9.0. + -. 0.7 cm.
Comparative example 2
(1) And (3) myzus persicae breeding: breeding myzus persicae by using tobacco seedlings;
(2) broad-band excellent aphid fly breeding: putting the egg collecting cup with the wide-band excellent aphid fly eggs into an artificial climate incubator for incubation; when the wide-band superior aphid fly eggs begin to hatch, feeding newly hatched larvae with myzus persicae until pupation occurs, and determining the feeding amount according to the breeding number of wide-band superior aphid flies; after pupating of broad band superior aphid fly larvae, taking the pupae out for centralized feeding, placing the pupae in an artificial climate box for hatching, and feeding the adult with 10% honey water and rape pollen.
The number of samples of this comparative example was 100, the pupation rate was 74%, the pupal eclosion rate was 90.5%, the growth history of the larvae was 9.0 + -1.6 days, the growth history of the pupae was 8.2 + -0.3 days, the length of the adult was 11.2 + -0.7 cm, and the wingspan was 10.8 + -0.5 cm.
The aphid breeding is labor-consuming and time-consuming, the labor cost is high, and the difficulty in regulating and controlling the temperature and humidity in the large-scale aphid breeding process is high, so that the aphid is easy to parasitize; compared with aphid feeding, the artificial feed feeding technology is simple, the feeding cost is low, and large-scale feeding can be realized in any area and laboratory.
The method for breeding wide-band optimized aphid fly provided by the invention is described in detail, the principle and the implementation mode of the invention are explained by applying specific examples, and the description of the examples is only used for helping to understand the method and the core idea of the invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, there may be variations in the specific embodiments and the application scope, and in summary, the content of the present specification should not be construed as a limitation to the present invention.
Claims (10)
1. An artificial feed for feeding wide-band superior aphid flies is characterized by comprising the following components in parts by weight: 25-35 parts of bee pupa; agar 0.5-1 min; 4-6 parts of beer yeast, 4-6 parts of cane sugar and 55-65 parts of water.
2. An artificial feed for feeding wide-band superior aphid flies is characterized by comprising the following components in parts by weight: 30 parts of bee pupa powder, 0.5 part of agar, 5 parts of beer yeast, 4.5 parts of cane sugar and 60 parts of water.
3. A preparation method of artificial feed for feeding wide-band superior aphid flies is characterized by comprising the following steps:
(1) weighing the bee pupa powder, agar, beer yeast, cane sugar and water according to the parts by weight of the claims 1-2;
(2) mixing bee pupa powder, beer yeast and sucrose into water with half volume of 25-30 ℃ to obtain solution A; dissolving agar in the other half volume of boiling water to obtain solution B;
(3) after the solution B was cooled to 60 ℃, A, B solution was mixed and left to stand until the artificial feed became semisolid.
4. A method for feeding wide-band superior aphid flies, characterized in that the wide-band superior aphid flies are fed by using the artificial feed of claim 1 as a food source.
5. The method for feeding wide-band superior aphid flies according to claim 4, wherein 3-year-old wide-band superior aphid flies are fed with the artificial feed of claim 1 as a feeding source.
6. The method for feeding wide-band myzus persicae as claimed in claim 5, comprising the steps of:
(1) wild collection and breeding of wide-band excellent aphids: collecting wild adult wide-band myzus persicae, putting into an insect rearing cage I, and feeding with 10% honey water and rape pollen;
(2) broad band optimal aphid fly oviposition pairing: placing the tobacco plant with the myzus persicae in an insect rearing cage II, then inoculating 1 pair of adult wide-band superior myzus persicae, and collecting egg granules every day when the wide-band superior myzus persicae lay eggs;
(3) hatching wide-band excellent aphid fly eggs: after the wide-band superior aphid flies lay eggs, taking out wide-band superior aphid fly eggs, placing the wide-band superior aphid fly eggs into an insect breeding box padded with filter paper, and placing the wide-band superior aphid fly eggs into an artificial climate box for hatching;
(4) feeding wide-band excellent aphid flies of 1-2 ages: when wide-band excellent aphid fly eggs begin to hatch, putting myzus persicae into the insect breeding box for breeding, and adding myzus persicae every day;
(5) feeding 3-year-old wide-band excellent aphids: after the wide-band superior aphid flies grow to 3 years old, artificial feed is put in for the wide-band superior aphid flies to take, the artificial feed is put in according to 1.5 times of the body shape of the wide-band superior aphid flies, and the artificial feed is replaced every day;
(6) collecting and eclosion of broad band superior aphid fly pupae: and after the broad-band superior aphid flies pupate, taking out the broad-band superior aphid fly pupae, putting the broad-band superior aphid fly pupae into an insect breeding cage III, then putting the broad-band superior aphid fly pupae into an artificial climate chamber, and waiting for the broad-band superior aphid flies to eclosion.
7. The method for feeding wide band superior aphid flies according to claim 6, wherein the feeding temperature of wide band superior aphid flies is 26 ± 1 ℃.
8. The method for feeding Aphidius gifuensis Ashmaed in claim 6, wherein the length, width and height of the first insect cage and the second insect cage are both 120cm by 120 cm; the length, width and height of the insect cage III are 30cm x 30cm-60cm x 60 cm.
9. The method for feeding wide-band superior aphid flies according to claim 6, wherein the number of the tobacco plant with myzus persicae in step (2) is more than 3000.
10. The method for feeding wide-band superior aphids and flies according to claim 6, wherein in step (4), myzus persicae is fed into the insect-raising box according to the ratio of heads of wide-band superior aphids and myzus persicae of 1:100 to 1: 150.
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