CN105028333A - Method for artificially and largely raising Dioryctria pryeri indoors - Google Patents
Method for artificially and largely raising Dioryctria pryeri indoors Download PDFInfo
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- CN105028333A CN105028333A CN201510357871.3A CN201510357871A CN105028333A CN 105028333 A CN105028333 A CN 105028333A CN 201510357871 A CN201510357871 A CN 201510357871A CN 105028333 A CN105028333 A CN 105028333A
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- cone
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- dioryctria
- berry size
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- 238000000034 method Methods 0.000 title claims abstract description 31
- 241000937887 Dioryctria Species 0.000 title abstract 9
- 241000238631 Hexapoda Species 0.000 claims abstract description 13
- 230000013011 mating Effects 0.000 claims abstract description 13
- 235000013601 eggs Nutrition 0.000 claims abstract description 10
- 239000012188 paraffin wax Substances 0.000 claims abstract description 8
- 244000045947 parasite Species 0.000 claims abstract description 7
- 235000021028 berry Nutrition 0.000 claims description 42
- 230000000384 rearing effect Effects 0.000 claims description 16
- 229920000742 Cotton Polymers 0.000 claims description 12
- 102000002322 Egg Proteins Human genes 0.000 claims description 12
- 108010000912 Egg Proteins Proteins 0.000 claims description 12
- 241000382353 Pupa Species 0.000 claims description 12
- 210000004681 ovum Anatomy 0.000 claims description 12
- 235000013351 cheese Nutrition 0.000 claims description 11
- 238000009395 breeding Methods 0.000 claims description 9
- 230000001488 breeding effect Effects 0.000 claims description 9
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 8
- 229930006000 Sucrose Natural products 0.000 claims description 8
- 238000005516 engineering process Methods 0.000 claims description 8
- 239000005720 sucrose Substances 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 239000004744 fabric Substances 0.000 claims description 7
- 241000018650 Pinus massoniana Species 0.000 claims description 6
- 230000006378 damage Effects 0.000 claims description 6
- 239000011521 glass Substances 0.000 claims description 6
- 230000002265 prevention Effects 0.000 claims description 6
- 230000019617 pupation Effects 0.000 claims description 6
- 230000001419 dependent effect Effects 0.000 claims description 4
- 230000003203 everyday effect Effects 0.000 claims description 4
- 235000013305 food Nutrition 0.000 claims description 4
- 238000005096 rolling process Methods 0.000 claims description 4
- 235000005638 Austrian pine Nutrition 0.000 claims description 3
- 235000008331 Pinus X rigitaeda Nutrition 0.000 claims description 3
- 240000000793 Pinus armandii Species 0.000 claims description 3
- 235000011612 Pinus armandii Nutrition 0.000 claims description 3
- 235000008565 Pinus banksiana Nutrition 0.000 claims description 3
- 235000011613 Pinus brutia Nutrition 0.000 claims description 3
- 241000018646 Pinus brutia Species 0.000 claims description 3
- 244000019397 Pinus jeffreyi Species 0.000 claims description 3
- 235000013264 Pinus jeffreyi Nutrition 0.000 claims description 3
- 240000007263 Pinus koraiensis Species 0.000 claims description 3
- 235000011615 Pinus koraiensis Nutrition 0.000 claims description 3
- 235000011609 Pinus massoniana Nutrition 0.000 claims description 3
- 235000008578 Pinus strobus Nutrition 0.000 claims description 3
- 235000011610 Pinus tabuliformis Nutrition 0.000 claims description 3
- 235000008585 Pinus thunbergii Nutrition 0.000 claims description 3
- 235000014030 Podocarpus spicatus Nutrition 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 235000017985 rocky mountain lodgepole pine Nutrition 0.000 claims description 3
- 239000004677 Nylon Substances 0.000 claims description 2
- 235000005205 Pinus Nutrition 0.000 claims description 2
- 241000218602 Pinus <genus> Species 0.000 claims description 2
- 241001236247 Pinus bungeana Species 0.000 claims description 2
- 235000000405 Pinus densiflora Nutrition 0.000 claims description 2
- 240000008670 Pinus densiflora Species 0.000 claims description 2
- 235000005456 Pinus sylvestris var mongolica Nutrition 0.000 claims description 2
- 241000114025 Pinus sylvestris var. mongolica Species 0.000 claims description 2
- 238000005520 cutting process Methods 0.000 claims description 2
- 238000002955 isolation Methods 0.000 claims description 2
- 229920001778 nylon Polymers 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 238000012545 processing Methods 0.000 abstract description 2
- 238000012258 culturing Methods 0.000 abstract 2
- 230000000266 injurious effect Effects 0.000 abstract 2
- 238000011534 incubation Methods 0.000 description 6
- 238000007726 management method Methods 0.000 description 6
- 238000011160 research Methods 0.000 description 5
- 235000013399 edible fruits Nutrition 0.000 description 3
- 230000001418 larval effect Effects 0.000 description 3
- 230000004071 biological effect Effects 0.000 description 2
- 238000007598 dipping method Methods 0.000 description 2
- 238000002224 dissection Methods 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 230000012447 hatching Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000003020 moisturizing effect Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000001932 seasonal effect Effects 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 238000010257 thawing Methods 0.000 description 2
- 241001074085 Scophthalmus aquosus Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a method for artificially and largely raising Dioryctria pryeri indoors and application of the method. The method comprises the following steps of wrapping various coniferous tree cones with paraffin to prepare feed for the Dioryctria pryeri; collecting, culturing and raising provenances, i.e., pupae of the Dioryctria pryeri, in the field; pairing newly emerged adult females and males to mate, and isolating female moths after mating for laying eggs; collecting and culturing the eggs of the Dioryctria pryeri until larvae hatch; transferring the hatched larvae to the cones wrapped by the paraffin and raising. According to the method disclosed by the invention, the Dioryctria pryeri is raised by the natural feed which is rich in source and low in cost, cannot be influenced by seasons and does not need a complicated processing course; the method is simple and easy, and can be suitable for largely raising the Dioryctria pryeri; a large amount of stable insect sources are provided for studies on the control technique for the Dioryctria pryeri and other injurious insects similar to the Dioryctria pryeri in injurious feature and production of parasites.
Description
Technical field
The present invention relates to foster worm technical field, especially relate to method and the application thereof of a kind of artificial room's mass rearing multiple pine cone insect Berry size.
Background technology
Berry size is the brill moth class pest of the multiple coniferous tree cones such as a kind of serious harm Korean pine and limb, serious generation causes that killed trees branch is withered, tree crown is lopsided, fruit is shrivelled often, have a strong impact on Tree height growth and fruit yield, not only serious harm is caused to Fast-growth phase, also affect seed harvest and standing forest natural regeneration, bring about great losses to ecotope and economic development.
The life of this worm major part completes in cone, because coniferous tree tree height is large, cannot study at woodland, observe its biological property, once this borer population is in the future dead after being plucked by cone, also do not carry out system research to its biological property therefore at present.
First the Study of Prevention Technology of often kind of insect will study its biology and physio-biochemical characteristics, however at present due to cannot in indoor a large amount of artificial feeding Berry size, lack experiment provenance, thus limit the research of its Prevention Technique.
The life of Berry size larval phase is hidden; the tip fruit that is injured produces the reaction of protectiveness resinosis; conventional dose control liquid is difficult to contact polypide and plays contact efficacy; therefore utilizing parasite to prevent and treat this worm is following research direction, and the research and apply of insect parasite needs a large amount of and stable Berry size as inoculation worm source.
Also there is no research or the Patents of Berry size artificial room mass rearing at present.
Summary of the invention
The object of the invention is to the defect overcoming prior art, a kind of method of artificial indoor rearing's Berry size is provided, with all kinds of coniferous tree cones with abundance and not by seasonal effect, do not need the indoor feeding Berry size of complex environment and equipment.
For achieving the above object, the present invention proposes following technical scheme: a kind of method of artificial indoor rearing's Berry size, comprises the following steps:
S1, with all kinds of coniferous tree cone of parafilm wrap process, prepare Berry size feed;
Provenance is raised in S2, field acquisition and cultivation;
S3, the adult male and female pairing by newly sprouting wings, mating, and the female moth isolation of post-coitum is laid eggs;
S4, collection the ovum cultivating Berry size are to larvae hatch;
S5, move on to the described larva hatched with parafilm wrap cone on raise.
Preferably, step S1 comprises:
S11, gather all kinds of annual coniferous tree cone in the wild, described annual coniferous tree cone comprises the combination of one or more in Chinese pine cones, masson pine cone, Cones of Pinus armandi, torch pine cone, Japanese red pine cone, cones of Pinus koraiensis, black pine cone, Pinus taiwanesis cone, pinus sylvestris var. mongolica cone, Chinese lace-bark pine cone;
S12, the described coniferous tree cone gathered is immersed the molten point melted is 50-60 DEG C of paraffin, and up and down, left and right rolling;
S13, the uniform described coniferous tree cone of taking-up parcel refrigerate stand-by under putting into 4 DEG C of-10 DEG C of temperature after naturally cooling.
Preferably, time to be used, prescind a joint by bottom the described coniferous tree cone after Treating Cuttings with Paraffin Wax, enter for young larva and take food.
Preferably, step S2 comprises: gather pupa in the killed cone in field after, and cultivate at the use for laboratory diameter 3cm-15cm biological culture dish of room temperature 25 ± 5 DEG C, humidity 70%-80%, each described biological culture dish puts into 1-20 head pupa.
Preferably, step S3 comprises:
S31, the adult male and female pairing of newly will sprout wings in described biological culture dish, and be released in mating in foursquare 21 order-70 order sarongs of length of side 10cm-30cm;
S32, the female moth of mating after 2 days to be isolated separately, and the described female moth of getting 2 post-coitums is put in a transparent jar together, described transparent jar main header casing 21 order-50 object nylon gauzes or 70 order-100 order cheese cloths, and place 1 70 order-100 order cheese cloth that 3-5cm is wide, 6-10cm is long in tank, for described female moth egg.
Preferably, put into a diameter 1cm-2cm, high 3cm-5cm and be the sucrose water of 5%-10% or the bottle of 3%-5% hydromel built with concentration in described sarong, described bottle bottleneck cotton mass jam-pack, described cotton mass top and bottleneck keep concordant.
Preferably, at the bottom of the tank of described transparent jar and bottle wall put into a diameter 7cm-9cm and the filter paper folded, and in tank, put into a diameter 1cm-2cm, high 3cm-5cm and be the sucrose water of 5%-10% or the bottle of 3%-5% hydromel built with concentration, described bottle bottleneck cotton mass jam-pack, described cotton mass top and bottleneck keep concordant.
Preferably, the ovum that the 1st day produces is moved on to rapidly on wet filter paper together with cheese cloth, then puts into culture dish and be cultured to larvae hatch.
Preferably, step S5 comprises: put into prescinding a joint bottom the cone of parafilm wrap the clear glass tank being placed with wet filter paper, larva is moved on on cone, 1 cone accesses 1 larva, within 1 week, change 1 wet filter paper, when after most of larvae pupation, filter paper and cone are together moved into dependent insect cage, the adult of emergence is proceeded to imago breeding cage according to step S3 and step S4 by every day again, for its mating and breeding.
A kind of method that present invention also offers artificial indoor rearing's Berry size is raised on a large scale at Berry size, the Study of Prevention Technology of Berry size and the similar insect of other Characteristics of Damage and produce the application providing worm source side face for parasite.
The invention has the beneficial effects as follows:
1, can utilize abundance, with low cost, not by seasonal effect, do not need the natural feed of complicated processing process (multiple coniferous tree cone) to raise Berry size, solve the technical bottleneck that Berry size in the past can not be raised in cone after harvesting;
2, this breeding technology process equipment is simple, can be suitable for Berry size and raise on a large scale, for Berry size and the Study of Prevention Technology of the similar insect of other Characteristics of Damage and the production of parasite provide stable and a large amount of worm source.
Embodiment
Clear, complete description is carried out to the technical scheme of the embodiment of the present invention below.
Embodiment 1:
1, the preparation of Berry size feed:
Gather the Chinese pine cones of annual health, masson pine cone, Cones of Pinus armandi totally 100, after cone after collection is taken back laboratory, the single molten point immersing thawing is in 50-60 DEG C of paraffin, up and down, left and right rolling, to be wrapped evenly after take out nature cooling after to put into 5 DEG C of refrigerator for cold for subsequent use.
2, Berry size raises field acquisition, the process of provenance:
Gather 200 and obtained 118 pupas by the cone that Berry size endangers altogether at indoor dissection cone, wherein male worm 49, female worm 69.Room temperature 26 ± 1 DEG C after male and female being separated, the use for laboratory diameter 9cm biological culture dish of humidity 75% is cultivated, and 12 pupas put into by each culture dish.40 male worms are had and 51 female worms normally sprout wings in whole incubation.
3, the raising of adult, management:
By adult male and female pairing of sprouting wings new in culture dish, and be released in the square sarong of length of side 30cm and make its pangamy.Put into a diameter 1cm in sarong, the bottle of high 5cm, bottle loads the sucrose water confession imago feeding that concentration is 5%, and bottleneck cotton mass jam-pack, stopper and bottleneck keep concordant.
The female moth 2 of mating after 2 days is only put in the clear glass tank of 300ml together, the fiber gauze of main header casing 1 32 mesh.For moisturizing with for Adult supplement nutrient, the filter paper of a diameter 9 centimetres being folded in half into right angle from centre puts at the bottom of tank and bottle wall, and puts into and fill the diameter 1cm that concentration is the sucrose water of 5%, the bottle of high 5cm, bottleneck cotton mass jam-pack, stopper and bottleneck keep concordant.Place 1 4cm in tank wide, the cheese cloth that 8cm is long, repeatedly roll over and supply Adult worms producting eggs several times.Whole incubation collects 1456 ovum altogether.
4, the collection of ovum, cultivation:
The ovum produced the 1st day moves on to rapidly together with cheese cloth the culture dish putting into diameter 9cm together with filter paper after on wet filter paper and is cultured to larvae hatch.987 egg hatching are had, average 6 days of ovum phase in whole incubation.
The management of 5, the raising of larva, pupa and adult:
By 4 with the clear glass tank prescinding a joint bottom the cone of parafilm wrap and put into together the 300ml being placed with wet filter paper.Dipping in wet writing brush with one moves on on cone by careful for the larva in culture dish, and 1 cone accesses 1 larva, and replacing in 1 week dips in wet filter paper 1 time.When cone will take food, put a fresh cone on old side at once and replace until larvae pupation, average 20 days of larval phase.When after most of larvae pupation, filter paper and cone are together moved into dependent insect cage, average 11 days of pupa time.The adult of emergence is proceeded to imago breeding cage, for its mating and breeding according to the raising of step 2 and step 3 adult, management method by every day again.Whole feeding process obtains adult 615 adults altogether, wherein male worm 287, female worm 328.
Embodiment 2:
1, the preparation of Berry size feed:
Gather 80 raw healthy black pine cones then, after the cone after collection is taken back laboratory, the molten point of single immersion thawing is in 50-60 DEG C of paraffin, upper and lower, left and right rolling, puts into 8 DEG C of refrigerator for cold for subsequent use after even rear taking-up nature to be wrapped cools.
2, Berry size raises field acquisition, the process of provenance:
Gather 180 and obtained 127 pupas by the cone that Berry size endangers altogether at indoor dissection cone, wherein male 61, female 66.Room temperature 28 ± 1 DEG C after male and female being separated, the use for laboratory diameter 9cm biological culture dish of humidity 70% is cultivated, and 10 pupas put into by each culture dish.49 male worms are had and 54 female worms normally sprout wings in whole incubation.
3, the raising of adult, management:
By adult male and female pairing of sprouting wings new in culture dish, and be released in the square sarong of length of side 40cm and make its pangamy.Put into a diameter 1.5cm in sarong, the bottle of high 8cm, bottle loads the sucrose water confession imago feeding that concentration is 10%, and bottleneck cotton mass jam-pack, stopper and bottleneck keep concordant.
Only put into together in the clear glass tank of 500ml by the female moth 4 of mating after 2 days, top 70 order cheese cloths cover, and tighten bottleneck with rubber band.For moisturizing with for Adult supplement nutrient, the filter paper of 2 diameters 7 centimetres being folded in half into right angle from centre puts at the bottom of tank and bottle wall, and puts into and fill the diameter 1.5cm that concentration is the sucrose water of 10%, the bottle of high 8cm, bottleneck cotton mass jam-pack, stopper and bottleneck keep concordant.Place 1 5cm in tank wide, the 70 order cheese cloths that 10cm is long, repeatedly roll over and supply Adult worms producting eggs several times.Whole incubation collects 1895 ovum altogether.
4, the collection of ovum, cultivation:
The ovum produced the 1st day moves on to rapidly together with cheese cloth the culture dish putting into diameter 9cm together with filter paper after on wet filter paper and is cultured to larvae hatch.1237 egg hatching are had, average 5 days of ovum phase in whole incubation.
The management of 5, the raising of larva, pupa and adult:
By 6 with the clear glass tank prescinding a joint bottom the cone of parafilm wrap and put into together the 500ml being placed with wet filter paper.Dipping in wet writing brush with one moves on on cone by careful for the larva in culture dish, and 1 cone accesses 1 larva.Replacing in 1 week dips in wet filter paper 1 time.When cone will take food, put a fresh cone on old side at once and replace until larvae pupation, average 17 days of larval phase.When after most of larvae pupation, filter paper and cone are together moved into dependent insect cage, average 11 days of pupa time.The adult of emergence is proceeded to imago breeding cage, for its mating and breeding according to the raising of step 2 and step 3 adult, management method by every day again.Whole feeding process obtains adult 877 adults altogether, wherein male worm 415, female worm 462.
Through test, the Berry size situation statistical form that female pairing continuous 4 generations raise:
The present invention is applicable to that Berry size is raised on a large scale, the Study of Prevention Technology of Berry size and the similar insect of other Characteristics of Damage and be applicable to produce for parasite provide worm source.
Technology contents of the present invention and technical characteristic have disclosed as above; but those of ordinary skill in the art still may do all replacement and the modification that do not deviate from spirit of the present invention based on teaching of the present invention and announcement; therefore; scope should be not limited to the content that embodiment discloses; and various do not deviate from replacement of the present invention and modification should be comprised, and contained by present patent application claim.
Claims (10)
1. a method for artificial indoor rearing's Berry size, is characterized in that, comprises the following steps:
S1, with all kinds of coniferous tree cone of parafilm wrap process, prepare Berry size feed;
Provenance is raised in S2, field acquisition and cultivation;
S3, the adult male and female pairing by newly sprouting wings, mating, and the female moth isolation of post-coitum is laid eggs;
S4, collection the ovum cultivating Berry size are to larvae hatch;
S5, move on to the described larva hatched with parafilm wrap cone on raise.
2. the method for artificial indoor rearing's Berry size according to claim 1, it is characterized in that, step S1 comprises:
S11, gather all kinds of annual coniferous tree cone in the wild, described annual coniferous tree cone comprises the combination of one or more in Chinese pine cones, masson pine cone, Cones of Pinus armandi, torch pine cone, Japanese red pine cone, cones of Pinus koraiensis, black pine cone, Pinus taiwanesis cone, pinus sylvestris var. mongolica cone, Chinese lace-bark pine cone;
S12, the described coniferous tree cone gathered is immersed the molten point melted is 50-60 DEG C of paraffin, and up and down, left and right rolling;
S13, the uniform described coniferous tree cone of taking-up parcel refrigerate stand-by under putting into 4 DEG C of-10 DEG C of temperature after naturally cooling.
3. the method for artificial indoor rearing's Berry size according to claim 2, is characterized in that, time to be used, prescind a joint, enter bottom the described coniferous tree cone after Treating Cuttings with Paraffin Wax take food for young larva.
4. the method for artificial indoor rearing's Berry size according to claim 1, it is characterized in that, step S2 comprises: gather pupa in the killed cone in field after, cultivate at the use for laboratory diameter 3cm-15cm biological culture dish of room temperature 25 ± 5 DEG C, humidity 70%-80%, each described biological culture dish puts into 1-20 head pupa.
5. the method for artificial indoor rearing's Berry size according to claim 4, it is characterized in that, step S3 comprises:
S31, the adult male and female pairing of newly will sprout wings in described biological culture dish, and be released in mating in foursquare 21 order-70 order sarongs of length of side 10cm-30cm;
S32, the female moth of mating after 2 days to be isolated separately, and the described female moth of getting 2-5 post-coitum is put in a transparent jar together, described transparent jar main header casing 21 order-50 object nylon gauzes or 70 order-100 order cheese cloths, and place 1 70 order-100 order cheese cloth that 3-5cm is wide, 6-10cm is long in tank, for described female moth egg.
6. the method for artificial indoor rearing's Berry size according to claim 5, it is characterized in that, put into a diameter 1cm-2cm, high 3cm-5cm in described sarong and be the sucrose water of 5%-10% or the bottle of 3%-5% hydromel built with concentration, described bottle bottleneck cotton mass jam-pack, described cotton mass top and bottleneck keep concordant.
7. the method for artificial indoor rearing's Berry size according to claim 5, it is characterized in that, at the bottom of the tank of described transparent jar and bottle wall put into a diameter 7cm-9cm and the filter paper folded, and in tank, put into a diameter 1cm-2cm, high 3cm-5cm and be the sucrose water of 5%-10% or the bottle of 3%-5% hydromel built with concentration, described bottle bottleneck cotton mass jam-pack, described cotton mass top and bottleneck keep concordant.
8. the method for artificial indoor rearing's Berry size according to claim 5, is characterized in that, is moved on to rapidly on wet filter paper by the ovum that the 1st day produces, then put into culture dish and be cultured to larvae hatch together with cheese cloth.
9. the method for artificial indoor rearing's Berry size according to claim 1, it is characterized in that, step S5 comprises: put into prescinding a joint bottom the cone of parafilm wrap the clear glass tank being placed with wet filter paper, moved on to by larva on cone, 1 cone accesses 1 larva, within 1 week, changes 1 wet filter paper, when after most of larvae pupation, filter paper and cone are together moved into dependent insect cage, and the adult of emergence is proceeded to imago breeding cage according to step S3 and step S4 by every day again, for its mating and breeding.
10. the application of the method for artificial indoor rearing's Berry size, it is characterized in that, be applicable to Berry size and raise, be applicable to the Study of Prevention Technology of Berry size and the similar insect of other Characteristics of Damage on a large scale and be applicable to produce for parasite provide worm source.
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| CN105494269A (en) * | 2015-12-29 | 2016-04-20 | 广西壮族自治区农业科学院甘蔗研究所 | Method for raising sugarcane chilo infuscatellus snellen |
| CN105532587A (en) * | 2016-03-13 | 2016-05-04 | 福建艳璇生物防治技术有限公司 | Anagasta kuehniella breeding method and application |
| CN107712481A (en) * | 2017-11-16 | 2018-02-23 | 东北林业大学 | The age of fir tip phycitid three to six ages big larvae feeds formula and preparation method thereof |
| CN108235970A (en) * | 2018-04-20 | 2018-07-03 | 吉林省林业科学研究院 | A kind of scale expanding propagation method for crossing length of side body cocoon bee |
| CN111264476A (en) * | 2020-03-12 | 2020-06-12 | 四川省农业科学院经济作物育种栽培研究所 | Predatory ladybug egg mass collecting and collecting method and device |
| CN112219797A (en) * | 2018-05-30 | 2021-01-15 | 吉林省林业科学研究院 | Application of cultured Chouioia cunea Yang in preventing and controlling Pinctada nubilalis and protecting Pinus koraiensis forest |
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