CN106954592B - Large-scale breeding method for Alliid lice spikemoss - Google Patents
Large-scale breeding method for Alliid lice spikemoss Download PDFInfo
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Abstract
The invention discloses a large-scale breeding method of Amikeia xylophila, which comprises the steps of cultivating and proliferating host plants capable of allowing diaphorina citri to grow, selecting host plants with good growth states, transferring the host plants to insect cages in a first isolation room, and releasing field collected diaphorina citri adults on each host plant to breed a large number of diaphorina citri nymphs; transferring the host plants bred with the diaphorina citri nymphs to insect cages of a second isolation room and a third isolation room respectively; releasing the field collected Aliesia longissima Miyaura into a second isolation room for breeding; and finally, transferring the arrhizus auritus branchicus bred in the second isolation room to a third isolation room for large-scale propagation, collecting the bred arrhizus auritus branchicus, and storing for later use. The large-scale breeding method has the advantages of continuous and cyclic process steps and annual breeding, the defects of large occupied space, high cost and the like of the traditional bee breeding method are overcome, and the bred Aliesia longissima small-horned bees are easy to collect and store.
Description
Technical Field
The invention belongs to the technical field of biological control, and particularly relates to a large-scale breeding method of Alimax carinica.
Background
Diaphorinaciti kuwayama, belonging to Hemiptera (Hemiptera) Psyllidae (Psyllidae), is one of the important pests harming the citrus industry in the world, and the damage mode mainly comprises direct damage and indirect damage. The diaphorina citri is mainly a rutaceous plant such as citrus and murraya paniculata, the new citrus tip emergence period is a peak period when the diaphorina citri is harmful, adults are scattered on tender shoot tips to absorb food as the harmful, nymphs are gathered and distributed on the tender shoot tips to absorb plant juice, so that the new leaves are dry and the new shoots drop, and meanwhile, the nymphs secrete a large amount of white honeydew, so that sooty mildew is easily caused in humid weather, and the photosynthesis of the plant is influenced.
According to the research, healthy seedlings can be infected by eating the germ-carrying imagoes on tender tips of oranges for more than 5 hours.
People mainly control the diaphorina citri by spraying chemical agents for a long time, however, the long-term and large-scale use of the chemical pesticides causes environmental pollution and harm to human health, and the drug resistance of insects is continuously enhanced. The alismoid trichogramma is a parasitic natural enemy of the diaphorina citri, eggs are laid in the diaphorina citri nymphs, and the larvae of the alismoid trichogramma suck the nutrition in the diaphorina citri nymphs during growth and development so as to eliminate the diaphorina citri.
However, the Aliesia lice small-horned wasp imagoes are difficult to artificially feed, long-time cold storage room storage cannot be realized, annual supply cannot be quantitatively and regularly realized, and the actual requirements of modern agriculture and forestry green production are difficult to meet. At present, no large-scale breeding process of the alismoid flea wasps is available. Therefore, the research and exploration of the large-scale breeding method of the Aleuropaea longissima small-jumping bees, which has the advantages of short breeding period, high efficiency, low cost and low energy consumption, is of great significance.
Disclosure of Invention
The invention aims to solve the technical problems that the adult Aliesia longissima Querchield is difficult to artificially feed, long-time cold storage room storage cannot be realized, full-year supply cannot be quantitatively and regularly realized, the actual requirements of green production of modern agriculture and forestry cannot be met and the like in the prior art, and provides a large-scale breeding method of the Aliesia longissima Querchield.
The invention aims to provide a large-scale breeding method of Alimax carinica.
The above object of the present invention is achieved by the following technical solutions:
a large-scale breeding method of Amikeia xylophila jumping bees comprises the steps of cultivating and proliferating host plants capable of allowing diaphorina citri to grow, selecting the host plants in good growth states, transferring the host plants to an insect rearing cage of a first isolation room, and releasing a plurality of field collected diaphorina citri adults on each host plant to breed a large number of diaphorina citri nymphs; transferring the host plants bred with the diaphorina citri nymphs to insect cages of a second isolation room and a third isolation room respectively; releasing a plurality of field collected Aliesia longissima Miyaura bees to a second isolation room for breeding; and finally, transferring the arrhizus auritus branchicus bred in the second isolation room to a third isolation room for large-scale propagation, collecting the bred arrhizus auritus branchicus, and storing for later use.
Preferably, the method specifically comprises the following steps:
s1, cultivating and managing host plants: placing a plurality of host plants capable of allowing the diaphorina citri to grow into a seedling preparation room, potting the host plants in artificially prepared mixed soil, and spraying the host plants with an insecticide and a mineral oil agent to remove harmful insects and mixed bacteria on the plants so as to ensure the healthy growth of the plants;
s2, proliferation and management of host plants: cutting off branches of a plurality of plants cultivated and managed in the step S1, carrying out cuttage propagation on the host plants, transferring the branches to a seedling greenhouse, removing apical meristems after the branches grow for a period of time to enable the branches to grow tender branches, and keeping the tender branches for later use after the branches grow well;
s3, breeding diaphorina citri nymphs: transferring the host plants cultured and well grown in the step S2 to an insect-raising cage of a first isolation room, and releasing a plurality of field collected adult diaphorina citri on each host plant to breed a large amount of diaphorina citri nymphs for later use;
s4, protection and transfer of diaphorina citri insect species: killing other harmful insects in the breeding process of the diaphorina citri nymphs at step S3, ensuring that the diaphorina citri nymphs which can be parasitized by the aleyrodids leioca exist on host plants, and transferring the plants to a third isolated room insect cage for later use;
s5, breeding the Aliesia lice spikelet: transferring a part of host plants which are cultured in the step S3 and grow with the diaphorina citri nymphs to a second isolated room insect cage, releasing a plurality of the early field collected small illicium macranthus in the isolated room insect cage, breeding the small illicium macranthus, and preparing for expanding the population of the small illicium macranthus;
s6, inoculating and culturing Allium praecox and Anhula variegata: transferring the alistiphaga triphaga bred in the step S5 to a third isolation room, and allowing the third isolation room to grow a host plant with the diaphorina citri nymphs through the step S4 so that the host plant can parasitize the diaphorina citri nymphs;
s7, collecting and storing Ali eating louse jumping bees: and collecting the Aliesia longissima Miyaura bred in the step S6, and storing for later use.
Preferably, the host plant in step S1 or S2 is murraya paniculata seedling, grapefruit, sour orange or wampee, which is free from diseases, strong, at least 30cm in height and at least 7mm in stem diameter, and has terminal bud removed, insecticidal sterilization pretreatment and lateral bud growth.
More preferably, the host plant for the growth of diaphorina citri is murraya paniculata seedling with a height of at least 30cm and a stalk diameter of at least 7 mm.
Specifically, in the host plant cultivation process, a seedling is potted in a flowerpot with the mixed soil being nutrient soil, namely perlite and vermiculite = 4: 2: 1, and is placed in a seedling preparation chamber for trimming, the temperature in the seedling preparation chamber is 10-30 ℃, RH60% -75%, the illumination is L: D is 14: 10, watering is carried out for 2 times every week, after trimming is completed, the harm of limousine, oyster, black bean aphid, ant and other mixed bacteria in the later growth process is prevented, an insecticide and a mineral oil agent are sprayed for maintenance, after the host plant grows for 3-4 months in the seedling preparation chamber, pruning and cuttage propagation are carried out on the host plant, after root powder is taken out and is potted in the mixed soil prepared manually, the plant after cuttage is transferred to a greenhouse for standby cultivation, the seedling is watered for 2 times every week, a 20-20-20 branch (N-P-K) compound fertilizer is applied once per month, so that new cuttage sprouts can grow as soon as possible, and 3-14 days later, the new sprouts can grow for 3 mm.
Preferably, in step S2, the temperature in the seedling greenhouse is maintained at 15-30 ℃, RH 60-75%, and illumination L: D is 14: 10.
Preferably, in step S2, after the host plant is trimmed and grows for 3-4 months, at least 5 shoots of each host plant grow, and each shoot has a length of 100mm or more.
Preferably, in step S3, each twig releases 3 adult diaphorina citri, i.e. at least 15 adult diaphorina citri per host plant, and after 2-4 days, each twig has 25-100 eggs of diaphorina citri, resulting in diaphorina citri species.
Preferably, in step S4, at least 70% of nymphs of 2-4 years old diaphorina citri exist on each host plant, and are parasitized by the aphidius gifuensis ashmead.
As a preferable implementation mode, in the invention, 8 insect cages with the length of 74.5cm, the width of 46.5cm and the height of 61.5cm are placed in each isolation room, and the insect cages are used for breeding the diaphorina citri and breeding and collecting the small sea bees of the Alicaria. The insect cage is made of an aluminum alloy frame, the bottom of the insect cage is covered by an airtight polypropylene plate, the periphery and the top of the insect cage are covered by gauze with the aperture of 0.87mm, light transmission and air permeation are guaranteed, one gauze can be adhered and taken down at will, and 10 host plants can be placed in each insect cage.
The device can facilitate placement of host plants and inoculation and collection of the Alhagus argyrophyllus poinsettii. Taking down the gauze which can be adhered to one surface at will, observing the development condition of the small sea lice fleshing bees, and collecting the eclosion bees by using an insect suction pipe to finish releasing the eclosion bees into a parasitic bee collecting bottle; the parasitic wasp which is not eclosion can be cut off together with the branch by scissors, and then the parasitic wasp is put into the parasitic wasp collecting box to be continuously cultured until eclosion occurs.
Preferably, in step S5, the collected alistiphaga micropellets are released into the insect cage of the second isolation room, an average of 5 alistiphaga micropellets per host plant is guaranteed, and after 7 days, the alistiphaga micropellets emerge from the parasitized diaphorina citri, so as to obtain the alistiphaga micropellet species.
Preferably, in step S6, the bred adult alistiphaga xylophila is transferred to a third isolation room insect cage, so that each insect cage has 30 alistiphaga xylophila.
Preferably, the temperature in the first isolation room, the second isolation room and the third isolation room is kept at 27 +/-2.5 ℃, RH 60-75%, the illumination period is L: D: 14: 10, and the illumination intensity is 5000-6000 lux.
Preferably, in step S7, the gauze which can be adhered to one side at will is removed, and the eclosion-induced alismoid ichthyophthirius is collected by an insect suction pipe and released into a parasitic wasp collecting bottle; the parasitic wasp which is not eclosion can be cut off together with the branch by scissors, and then the parasitic wasp is put into the parasitic wasp collecting box to be continuously cultured until eclosion occurs.
Preferably, the collection bottle containing the parasitic wasps is stored in a refrigerator at 17 ℃ and RH60% -75%.
In addition, in the method of the present invention, the diaphorina citri in the first isolation chamber requires field collection and replenishment each year to prevent population degradation; the host plant seedlings removed from the third isolation room can be trimmed again and put back into the seedling preparation room for recycling.
The method provided by the invention can ensure that the survival rate of the Aliesia longissima Miq is capable of meeting the actual demand by providing the nutrition required by the development of the Aliesia longissima Miq. The large-scale breeding method has the advantages of continuous and cyclic process steps and annual breeding, the defects of large occupied space, high cost and the like of the traditional bee breeding method are overcome, and the bred Aliesia longissima small-horned bees are easy to collect and store. The problem that the small sea cucumbers of the Alimazada cannot be stored in a refrigerating chamber for a long time and cannot be supplied all year round in a quantitative and timed mode is solved, and the problem that the supply of the diaphorina citri in agriculture and forestry is insufficient is solved. The industrialization aim of popularization and application of the biological control technology from laboratories to fields is fulfilled.
Compared with the prior art, the invention has the following beneficial effects:
according to the large-scale breeding method of the small arrestis trichogrammae, provided by the invention, host plants, diaphorina citri nymphs and small arrestis trichogrammae seeds are ensured to be provided all the year round through a controllable greenhouse; the inoculation and breeding technology of the diaphorina citri and the inoculation and breeding technology of the Alhagia bigarensis provided by the invention can ensure the annual large-batch supply of the Alhagia bigarensis, and has the advantages of continuous circulation of process steps and annual breeding of the Alhagia bigarensis.
In addition, the method provided by the invention has the advantages of short breeding period, high efficiency, low cost and low energy consumption, and the bred Aliesia longissima Miaotiae has superior quality, so that the problem of insufficient supply of the agricultural and forestry prevention and control diaphorina citri is solved, and the industrialized aim of popularization and application of the biological prevention and control technology from a laboratory to a field is fulfilled.
Drawings
Fig. 1 is a large-scale production flow chart of the Ali eating lice jumping bees of the invention.
Detailed Description
The invention is further described with reference to the drawings and the following detailed description, which are not intended to limit the invention in any way. Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated.
Unless otherwise indicated, reagents and materials used in the following examples are commercially available.
Example 1 Large-Scale reproduction method of Amikebacopa prionitis
A large-scale breeding method of Aliesia lice spikemoss is characterized by comprising the following steps:
s1, cultivating and managing host plants
The method comprises the steps of purchasing a disease-free strong murraya paniculata seedling with the height of at least 30cm and the stem diameter of at least 7mm on the market, potting the murraya paniculata seedling in mixed soil prepared by artificial nutrition, namely perlite and vermiculite = 4: 2: 1, placing the mixed soil in a seedling preparation chamber, pruning the mixed soil to promote the germination of new buds, preventing the hazards of the limousine, the orange cracky, the black bean aphid, the ant and other bacteria in the later growth process after pruning, spraying insecticide and mineral oil for maintenance, watering the mixed soil for 2 times per week in the seedling preparation chamber at the temperature of 10-30 ℃, at the RH 60-75%, under the illumination of L D ═ 14: 10.
Pruning the murraya paniculata seedlings once every 3-4 months after transplantation or transplanting new standby seedlings to ensure that the murraya paniculata seedlings are ready for use all the year round.
In addition, the host plant murraya paniculata is a perennial plant, and varieties with strong disease resistance and weak insect resistance are required to be selected, the height of the varieties is strictly controlled, and trimming is carried out in time, so that the breeding of the diaphorina citri is facilitated.
S2, propagation and management of host plants
Shearing a murraya jasminorage branch which grows well after 3-4 months in a nursery stock preparation room, dipping root powder on the root of the murraya jasminorage branch into mixed soil with a formula of nutrient soil, namely perlite and vermiculite = 4: 2: 1, cutting and potting the murraya jasminorage branch in a nursery stock greenhouse, transferring the mixed soil into the nursery stock greenhouse for continuous culture, enabling each murraya jasminorage seedling to grow tender branches after removing apical meristem at least 5, 3-14 days, germinating root buds, growing the new buds to be 1-3 mm long, enabling the length of the new buds to be more than 100mm after 3-4 months, controlling the temperature in the nursery stock greenhouse to be 15-30 ℃, RH60% -75%, lighting L: D ═ 14: 10, watering 2 times every week, and applying 20-20 (N-P-K) compound fertilizer once every month.
S3, breeding of diaphorina citri nymphs
Transferring the host plants cultured and well grown in the step S2 to an insect rearing cage of a first isolation room, releasing at least 3 adult diaphorina citri on each twig of the murraya paniculata seedlings, namely at least 15 adult diaphorina citri on each host plant, and after 2-4 days, obtaining diaphorina citri seeds by allowing 25-100 eggs of diaphorina citri on each twig. 8 insect cages with the length of 74.5cm, the width of 46.5cm and the height of 61.5cm are placed in the first isolation room, and 10 murraya jasminorage seedlings proliferated in the step S2 are placed in each insect cage.
The temperature in the first isolation chamber is kept at 27 +/-2.5 ℃, RH is 60% -75%, the illumination period is L, D is 14: 10, and the illumination intensity is 5000-6000 lux.
S4, protection and transfer of diaphorina citri insect species
Killing other harmful insects in the breeding process of the diaphorina citri nymphs at step S3, ensuring that at least 70% of the nymphs in 2-4 years of diaphorina citri exist on each murraya paniculata seedling for parasitizing the trichogramma aurantiaca, and transferring the plant to a third isolation room for later use;
and 8 insect cages with the length of 74.5cm, the width of 46.5cm and the height of 61.5cm are placed in the third isolation room, and 10 murraya paniculata seedlings growing with the diaphorina citri nymphs are placed in each insect cage in the step S3.
The temperature in the third isolation chamber is kept at 27 +/-2.5 ℃, RH is 60% -75%, the illumination period is L: D is 14: 10, and the illumination intensity is 5000-6000 lux.
S5, breeding of Aliesia horrida and ichthyophthirius multifiliis
Transferring a part of host plants which are cultured in the step S3 and grow with the diaphorina citri nymphs to a second isolation room insect cage, releasing a plurality of the early field collected Aliskipper small bee imagoes to the insect cage, ensuring that each Murraya paniculata seedling has 5 Aliskipper small bee imagoes on average, and after 7 days, eclosion of the parasitized diaphorina citri small bees to obtain Aliskipper small bee species, so as to prepare for expanding the population of the Aliskipper small bee species;
8 insect cages with the length of 74.5cm, the width of 46.5cm and the height of 61.5cm are placed in the second isolation room, and 10 murraya paniculata seedlings growing with diaphorina citri nymphs are placed in each insect cage in the step S3.
The temperature in the second isolation chamber is kept at 27 +/-2.5 ℃, RH is 60% -75%, the illumination period is L: D is 14: 10, and the illumination intensity is 5000-6000 lux.
S6, inoculating and cultivating Allium practicum and small-jump bees
Transferring the plurality of the Aliskipper small-lice jumping bee adults bred in the step S5 to a third isolation room, and growing host plants with the diaphorina citri nymphs through the step S4 to ensure that each insect rearing cage has 30 Aliskipper small-lice adult adults so that the adult adults parasitize the diaphorina citri nymphs;
s7, collecting and storing Aliesia lice jumping bees
Taking down the insect-raising cage of the third isolation room, and sticking a gauze on one surface at will, wherein the eclosion eating lice jumping wasps are collected by an insect suction pipe and released into a parasitic wasp collecting bottle; the parasitic wasps which are not eclosion can be cut off together with the branches by scissors, and then the parasitic wasps are put into the parasitic wasp collecting box and continuously cultured until the parasitic wasps are eclosion and then are stored in a collecting bottle.
Storing the collecting bottles containing the parasitic wasps in a refrigerator with the temperature of 17 ℃ and the RH60% -75%, taking out the collecting bottles containing the parasitic wasps every 5 days, placing the collecting bottles in an environment with the temperature of 25 ℃ for 3 hours, putting filter paper strips dipped with honey water with the concentration of 20% into each collecting bottle, providing nutrition for the parasitic wasps, and immediately placing the collecting bottles in the refrigerator with the temperature of 17 ℃ after the nutrition is provided.
2. When the citrus psylla is required to be controlled, the collecting bottle is taken out from the refrigerating chamber, adult bees of the Aliesia longissima Miq-jump wasps are collected from the collecting bottle by using the portable insect suction pipe, and the adult bees are released to an agricultural and forestry area harmed by the citrus psylla, so that the control of the citrus psylla can be realized.
Example 2 host plant selection
1. The specific steps of the large-scale breeding method of the alismoid flea wasps are the same as those of the embodiment 1, and the only difference is that host plants are grapefruit, sour orange and wampee respectively.
2. The results are shown in Table 1
TABLE 1 comparison of Adaptation parameters of diaphorina citri on different host plants
| Host plant | Egg-imago (d) | Survival rate (%) | Average life (d) | Sex ratio (%) |
| Root of common jasminorange | 16.42 | 51.74 | 46.88 | 0.50 |
| Shaddock | 17.10 | 53.79 | 48.00 | 0.50 |
| Sour orange | 16.80 | 47.13 | 46.13 | 0.49 |
| Clausena lansium (lour.) Merr | 16.13 | 41.23 | 42.75 | 0.50 |
3. Comprehensive comparison
TABLE 2 diaphorina citri biotope biological tables on different host plants
| Host plant | Net growth rate of generation (R)o） | Intrinsic rate of increase (r)m） | Generation average lifetime (T) | Cycle growth rate (lambda) |
| Clausena lansium (lour.) Merr | 179.07 | 0.1306 | 39.73 | 1.1396 |
| Sour orange | 174.31 | 0.1298 | 39.77 | 1.1386 |
| Clausena lansium (lour.) Merr | 145.27 | 0.1311 | 37.98 | 1.1401 |
| Root of common jasminorange | 179.45 | 0.1337 | 38.79 | 1.1431 |
In conclusion, the development time of the diaphorina citri ovum-imago on the murraya jasminorage seedlings is relatively short, the survival rate is relatively high, and the sex ratio has no obvious difference. The intrinsic growth rate of the diaphorina citri on the murraya paniculata seedling is the highest, and the murraya paniculata seedling is most suitable for the development and propagation of the diaphorina citri population by comprehensively comparing the diaphorina citri life table parameters on different host plants.
Example 3 illumination condition optimization
1. The specific steps of the large-scale breeding method of the alismoid flea wasps are the same as those in example 1, and the only difference is that the illumination conditions in the diaphorina citri insect breeding room are different.
2. The results are shown in Table 3
TABLE 3 comparison of the Adaptation parameters of diaphorina citri under different lighting conditions (host plant Murraya koenigii)
| Light conditions | Egg-imago (d) | Survival rate (%) | Average life (d) | Sex ratio (%) |
| L：D＝16：8 | 16.32 | 47.68 | 46.88 | 0.49 |
| L：D＝14：10 | 17.04 | 47.74 | 46.91 | 0.50 |
| L：D＝12：12 | 16.45 | 47.71 | 45.74 | 0.50 |
| L：D＝10：14 | 16.52 | 46.83 | 45.71 | 0.47 |
| L：D＝8：16 | 16.42 | 45.97 | 44.83 | 0.50 |
3. Comprehensive comparison
TABLE 4 diaphorina citri biological watch under different lighting conditions (host plant is murraya paniculata)
| Light conditions | Net growth rate of generation (R)o） | Intrinsic rate of increase (r)m） | Generation average lifetime (T) | Cycle growth rate (lambda) |
| L：D＝16：8 | 179.03 | 0.1333 | 38.77 | 1.1431 |
| L：D＝14：10 | 179.07 | 0.1337 | 38.79 | 1.1433 |
| L：D＝12：12 | 177.97 | 0.1329 | 38.31 | 1.1427 |
| L：D＝10：14 | 173.21 | 0.1322 | 37.76 | 1.1377 |
| L：D＝8：16 | 168.32 | 0.1279 | 36.81 | 1.1124 |
In conclusion, the diaphorina citri ovum-imago has relatively short development time and relatively high survival rate when the illumination condition in the insect breeding room is L: D14: 10, and the sex ratio is not obviously different, the intrinsic growth rate of the diaphorina citri is the highest when the illumination condition is L: D14: 10, and the illumination condition of L: D14: 10 is most suitable for the development and propagation of the diaphorina citri population by comprehensively comparing the life surface parameters of the diaphorina citri under different illumination conditions.
Example 4 selection of host age of Ceriporiopsis irica
1. The specific steps of the large-scale breeding method of the Aliesia longissima Queenwich are the same as those in the embodiment 1, and the only difference is that the Aliesia longissima Queenwich is provided with different host ages.
2. The results are shown in Table 5
TABLE 5 selection of Aliskipper flea bees for host nymphs of different ages
| Age of host | Test sample size (head) | Daily egg laying amount (granule) | Female sex ratio (male/N) | Difference of specimen |
| Two years old | 12 | 0～7 | 1.8 | 1.7 |
| Three years old | 13 | 0～12 | 3.0 | 2.1 |
| Four ages | 9 | 0～12 | 2.7 | 2.0 |
Non-selective test results show that the small arrophagus nudus can parasitize second-age, third-age and fourth-age diaphorina citri nymphs, the daily egg laying amount of the third-age and fourth-age nymphs is higher than that of the third-age nymphs, and therefore the small arrhagus nudus prefers to parasitize third-age and fourth-age diaphorina citri nymphs.
Example 5 comparison of size of individuals after emergence of parasitizing hosts of different ages
1. The specific steps of the large-scale breeding method of the Aliesia longissima Miq.C.Smith are the same as those in example 1, and the only difference is the size of individuals after host emergence of different ages are parasitized.
2. The results are shown in Table 6
TABLE 6 comparison of sizes of individuals after emergence of hosts of different ages
| Age of host | Measuring the sample size | Hindfoot shank size (mm) | Standard deviation of |
| Two years old | 25 | 0.338 | 0.026 |
| Three years old | 56 | 0.350 | 0.024 |
| Four ages | 46 | 0.344 | 0.024 |
The individual with large body size has long service life, strong fecundity and good flight diffusion capacity, and the data show that: the individual size of the Aleuropaea longissima Queensis after emergence of three-year and four-year old diaphorina citri nymphs is superior to that of the Aleuropaea Queensis after emergence of two-year old diaphorina citri nymphs.
Claims (1)
1. A large-scale breeding method of Amikebacea, which is characterized by comprising the following steps:
s1, cultivating host plants: placing a plurality of host plants capable of allowing the diaphorina citri to grow into a seedling preparation room, potting the host plants in artificially prepared mixed soil, and spraying the host plants with an insecticide and a mineral oil agent to remove harmful insects and mixed bacteria on the plants so as to ensure the healthy growth of the plants;
s2, proliferation of host plants: cutting off branches of a plurality of plants cultivated and managed in the step S1, carrying out cuttage propagation on the host plants, transferring the branches to a seedling greenhouse, removing apical meristems after the branches grow for a period of time to enable the branches to grow tender branches, and keeping the tender branches for later use after the branches grow well;
s3, breeding diaphorina citri nymphs: transferring the host plants cultured and well grown in the step S2 to an insect-raising cage of a first isolation room, and releasing a plurality of field collected adult diaphorina citri on each host plant to breed a large amount of diaphorina citri nymphs for later use;
s4, protection and transfer of diaphorina citri insect species: killing other harmful insects in the breeding process of the diaphorina citri nymphs at step S3, ensuring that the diaphorina citri nymphs which can be parasitized by the aleyrodids leioca exist on host plants, and transferring the plants to a third isolated room insect cage for later use;
s5, breeding the Aliesia lice spikelet: transferring a part of host plants which are cultured in the step S3 and grow with the diaphorina citri nymphs to a second isolated room insect cage, releasing a plurality of the early field collected small illicium macranthus to the second isolated room insect cage, and breeding the small illicium macranthus;
s6, inoculating and culturing Allium praecox and Anhula variegata: transferring the alistiphaga triphaga bred in the step S5 to a third isolation room, and allowing the third isolation room to grow a host plant with the diaphorina citri nymphs through the step S4 so that the host plant can parasitize the diaphorina citri nymphs;
s7, collecting and storing Ali eating louse jumping bees: collecting the Aliesia longissima Miyaura bred in the step S6, and storing for later use;
the diaphorina citri nymphs are three-year-old or four-year-old diaphorina citri nymphs;
in the step S1 or S2, the host plant is murraya paniculata seedling which is free of diseases, strong, at least 30cm in height and at least 7mm in stem diameter, and has terminal bud removed, insect killing and sterilization pretreatment and lateral bud growing;
in the step S1, the temperature in the nursery stock preparation room is 10-30 ℃, RH 60-75% and the illumination ratio is L: D is 14: 10;
the formula of the mixed soil artificially prepared in the step S1 is nutrient soil: perlite: vermiculite 4: 2: 1;
in the step S2, the temperature in the nursery stock greenhouse is 15-30 ℃, RH 60-75%, and the illumination ratio is L: D is 14: 10;
after the host plants are pruned in the step S2, at least 5 shoots grow out of each host plant, and the length of each shoot is more than 100 mm;
in the step S3, 3 citrus psyllid adults are released from each twig of the host plant, namely at least 15 citrus psyllid adults are released from each twig of the host plant, and after 2-4 days, 25-100 citrus psyllid eggs are obtained from each twig, namely, the citrus psyllid is a species of the citrus psyllid;
at least 70% nymphs of 2-4 years old diaphorina citri exist on each host plant in the step S4;
the temperature in the first isolation room, the second isolation room and the third isolation room is kept at 27 +/-2.5 ℃, RH is 60% -75%, the illumination period is L: D is 14: 10, the illumination intensity is 5000-6000 lux, 8 insect cages are placed in each isolation room, and 10 host plants are placed in each insect cage.
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| CN108207822B (en) * | 2018-03-12 | 2020-10-02 | 内蒙古农业大学 | Artificial mass rapid breeding technical method for Aphidius fusceolatus |
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| CN113519466A (en) * | 2021-08-05 | 2021-10-22 | 广州威佰昆生物科技有限公司 | Method for feeding diaphorina citri by tissue culture murraya paniculata |
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| CN106106374A (en) * | 2016-06-30 | 2016-11-16 | 华南农业大学 | A kind of biological control method of bright abdomen glaze chalcid fly |
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