CN113558014B - Large-scale production method of dormitory lice flea bees - Google Patents
Large-scale production method of dormitory lice flea bees Download PDFInfo
- Publication number
- CN113558014B CN113558014B CN202110954504.7A CN202110954504A CN113558014B CN 113558014 B CN113558014 B CN 113558014B CN 202110954504 A CN202110954504 A CN 202110954504A CN 113558014 B CN113558014 B CN 113558014B
- Authority
- CN
- China
- Prior art keywords
- diaphorina citri
- parasitic
- population
- bees
- diaphorina
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The invention relates to the field of biological control of agricultural pests, and discloses a large-scale production method of an Alhagia arguta, which mainly comprises the following four steps: (1) establishing an doripes alfa flea population; (2) preparing a diaphorina citri to be parasitized population; (3) Inoculating the parasitic wasp of the diaphorina citri-Alhagogue wasp into the diaphorina citri to-be-parasitic population; (4) collecting parasitic wasps. The large-scale production method of the dormitory-louse wasps has simple flow, controllable breeding scale and annual propagation work without season limitation; meanwhile, parasitic bees are concentrated in emergence time, the population quantity is controllable, no psyllium interference is needed to be considered during bee collection, and the operation is simple; meanwhile, the method has no special hard requirement on the type and size of the host plant, is suitable for all the host plants of the diaphorina citri, and has high utilization rate of the host plants.
Description
Technical Field
The invention relates to the field of biological control of agricultural pests, in particular to a large-scale production method of doriphora.
Background
In recent decades, china has been directed to diaphorina citriDiaphorina citriResearch reports of parasitic wasps mainly focus on bright abdominal enamel waspsTamarixia radiataAnd the alishi lutiao waspsDiaphorencyrtus aligarhensis Two kinds.
The earliest distribution of dorhage lice-jumping bees was recorded in india, by shaffee in 1975. As the same as the bright abdomen glaze wasps are parasitic wasps of the diaphorina citri, the method is quickly used for biological control of the diaphorina citri. The targeted introduction and utilization in China starts in Taiwan area around 1995, and related documents in continental areas are mainly reported in foreign researches and recorded in China sporadic distribution, but the quantity tends to increase.
Around 2000, american researchers began to introduce the ari lice-jumping puppies from taiwan and vietnam in florida, and in 2006, introduced a batch of ari lice-jumping puppies population from guangdong, which was then further introduced into southern citrus production areas such as california and texas. The 2014 related personnel in turn collected an amphiphilically propagated population from pakistan, which was introduced into california. By 2017, researchers have released about 28 ten thousand foodflies in the southern region of the united states, but are still available for colonisation and further monitoring.
The inhibition effect of the dormitory flea bees on the diaphorina citri is verified in the areas of Pakistan, retaining the NIANWANGU island, taiwan island, sauter Arabian, the south of the United states and the like, but the control effect of the dormitory flea bees in the American introduction area is inferior to that of the Liang Zhou glaze bees.
Regarding the biological control of diaphorina citri by utilizing the diaphorina citri, china is mainly remained on the aspect of reporting the utilization of foreign natural enemies at present, no public report cases of artificial propagation and release are found in China, and no biological experiment report is found.
Citrus belongs to important cash crops in south China, and chemical pesticides are mainly used for controlling diaphorina citri at present. Compared with research progress at home and abroad, along with the transition of agriculture in China to a sustainable development mode, the biological control technology such as parasitic wasps is utilized to meet the rapid development period. Therefore, development of a large-scale production method of the apricots for biologically controlling diaphorina citri is needed.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a large-scale production method of the Aleuritophaea e, which can rapidly, regularly and quantitatively obtain Aleuritophaea e pupa and adults in any time period throughout the year, can adjust the raising scale at any time according to the expected propagation target, and has high activity of the obtained parasitic wasps.
In order to achieve the above purpose, the technical scheme provided by the invention is as follows:
a large-scale production method of an alishi tiao bee comprises the following steps:
(1) Establishing an alishi lutiao population: the method comprises the steps of (1) raising diaphorina citri host plants, diaphorina citri and diaphorina citri flea bees in a relatively isolated environment to form a mixed population, ensuring stable raising conditions, ensuring stable growth vigor of the diaphorina citri host plants, and maintaining a sufficient number of parasitic bees;
(2) Preparing a diaphorina citri to be parasitized population: placing healthy host plants in a rearing cage, and promoting tender shoots to occur through pruning and water and fertilizer control; when tender buds of host plants grow to be suitable for the diaphorina citri to spawn (different host plants have differences, such as large leaf plants of citrus reticulata, etc. grow to have tender buds of about 3cm, small leaf plants of Murraya paniculata, etc. grow to have tender buds of about 2 cm), selecting the diaphorina citri adults in the step (1), putting the diaphorina citri adults into a feeding cage, and taking out all adults after the diaphorina citri spawns on tender tips for 2-4 days; continuously breeding diaphorina citri eggs in the cages, and inoculating parasitic wasps when the eggs develop to a proper stage;
(3) Inoculating parasitic wasps: collecting the adult dorifer flea beetles in the step (1), feeding the adult dorifer flea beetles into the prepared diaphorina citri to be parasitized population, and timely removing newly emerged diaphorina citri adults during feeding;
(4) Collecting parasitic wasps: when the population in the step (3) starts to have the pupation or the eclosion of the Alhaga lupeus, collecting parasitic wasps, wherein the collected new parasitic wasps can be used for experiments, manually released for biological control or supplementing a germplasm library and the like.
As a further improvement of the above scheme, in the step (4), after the parasitic wasps are collected, the host plant may be reused.
As a further improvement of the above scheme, the number of successive repeated uses of the host plant is not more than 3.
As a further improvement of the above scheme, in the step (1), ensuring stable feeding conditions, stable growth vigor of the diaphorina citri host plant, and maintaining a sufficient number of parasitic bees specifically includes: 1) Controlling the ambient temperature to be 25-30 ℃, the humidity to be 45-75%, the illumination time to be 12-16 hours, and the illumination intensity in the middle of the plant to be more than 3000lx; 2) Controlling water and fertilizer management, ensuring good growth vigor of host plants and stable tender shoot occurrence quantity; 3) Controlling the population quantity of non-target organisms, and preventing the non-target organisms from obviously affecting plant growth or occupying ecological niches of diaphorina citri and parasitic wasps; 4) And (5) collecting the fowls in the field and supplementing the population quantity.
As a further improvement of the above, the non-target organisms include insects, mites, germs.
As a further improvement of the above-described scheme, in the step (1), the relatively isolated environment means a separate feeding area, which is separated from feeding areas such as a feeding cage area in other steps.
As a further improvement of the scheme, in the step (2), the size of the feeding cage can be adjusted according to feeding conditions and plant size, but six-sided net coverage is required, the light transmittance is good, the illumination intensity in the middle of the plant is more than 3000lx, the mesh number of the net is more than 50 meshes, the aperture is less than 0.28mm, the side face (long x high face) is provided with 1 opening for daily management, and the top (long x wide face) is provided with 2 openings for collecting the adult dormitoris.
As a further improvement of the above scheme, in the step (2), the plant water and fertilizer control includes: and the concentrated emergence of new buds is promoted by pruning, watering and fertilizing.
As a further improvement of the scheme, in the step (2), the number of the access of the diaphorina citri adults is 2-3 per tender tip; the suitable stages are: when the nymphs of the diaphorina citri are consistent in age, the 3-age nymphs are the proper stage; when the nymphs of the diaphorina citri are inconsistent in age, the 3-year nymphs are at a proper stage when the ratio is more than 30%.
As a further improvement of the above scheme, in the step (3), the number of the access of the ariigera flea beetles depends on the number of the diaphorina citri, and the following conditions are satisfied: the number ratio of female Alhagia arguta adult to diaphorina citri is 1:15-30.
The invention provides a system method suitable for propagation of the dormitory-lice flea bees on the basis of overseas biological reports on the dormitory-lice flea bees and known indoor feeding experiment records at home and abroad. The method can rapidly, regularly and quantitatively obtain the dormitory-hopped bee pupae and adults in any time period throughout the year, can adjust the raising scale at any time according to the expected propagation target, and has high activity of the obtained parasitic wasps.
Compared with the prior art, the invention has the beneficial effects that:
1. the full propagation process of the large-scale production method provided by the invention only needs 2 independent areas at the minimum, and 4 main operation steps are needed, so that the process is simple, the propagation work can be carried out all the year round, the season limitation is avoided, the propagation speed is high, and the activity of the obtained parasitic wasps is high;
2. the breeding scale is controllable, the minimum unit for propagation is 1 plant in 1 feeder cage, and the scale can be enlarged by simple repetition; meanwhile, the breeding cages are not interfered with each other, and the breeding scale can be flexibly controlled according to actual production conditions;
3. the nymphs of the diaphorina citri are uniform in age and controllable in population quantity, so that parasitic wasps are concentrated in eclosion time and controllable in population quantity; meanwhile, the emergence period of the diaphorina citri and the parasitic wasp is not coincident, the diaphorina citri interference is not required to be considered when the wasp is collected, and the operation is simple;
4. the utilization rate of the host plant is high, and after one propagation period is finished, the host plant keeps a healthy state, and can be put into propagation in the next period without additional operation; in the preferred scheme, the operation during the development of the diaphorina citri can be greatly simplified by increasing the inoculation amount of the parasitic wasps;
5. the method has no special hard requirement on the type and size of the host plant, and is suitable for all hosts of the diaphorina citri, so that the host plant can be flexibly selected according to the actual planting conditions and habits.
Drawings
FIG. 1 is a graph showing the statistical result of daily emergence of diaphorina citri and Alhagia arguta in example 1 of the present invention;
FIG. 2 is a graph showing the statistical result of daily emergence of diaphorina citri and Alhagia arguta in example 2 of the present invention.
Detailed Description
For a better description of the objects, technical solutions and advantages of the present invention, the present invention will be further described with reference to the following specific examples.
A large-scale production method of an alishi tiao bee comprises the following steps:
(1) Establishing an alishi lutiao population:
and (3) breeding the mixed population of the diaphorina citri host plant, the diaphorina citri and the parasitic wasp in a relatively isolated environment, so that the diaphorina citri or the parasitic wasp cannot migrate or spread into the breeding environment of the subsequent steps.
Maintaining the room temperature of the raising environment at 25-30 ℃, the humidity at 45-75%, the illumination time length at 12-16 hours, the illumination intensity at the middle part of the plant being more than 3000lx, keeping the soil moist, moderately topdressing and pruning, and stabilizing the growth vigor of the host plant; the population quantity of non-target organisms such as whiteflies, spider mites, powdery mildew fungi and the like is controlled in a physical or chemical mode, so that the influence on the survival of host plants, diaphorina citri or parasitic wasps is prevented; ensuring the requirement of continuously supplying the subsequent steps for a sufficient number of dorifer flea bees; the population scale can be increased or decreased according to the propagation targets, and the population can be rapidly expanded by a field collection or small-scale propagation mode.
(2) Preparing a diaphorina citri to be parasitized population:
placing a raising cage in a relatively isolated area, wherein the raising cage has proper required size, 1-2 pots of host plants can be placed, 6-surface net coverage is realized, the mesh number is more than 50 meshes (aperture is less than 0.28 mm), the illumination intensity of the middle part of the plants in the cage is ensured to be more than 3000lx, an opening is reserved on the side surface for daily management, and 2 parasitic wasp collecting openings are arranged at the top; selecting healthy and insect-free host plants, placing the host plants in a cage for management, promoting the concentrated generation of tender shoots through concentrated pruning and water and fertilizer control, and applying the plant to the spawning of psyllids when the tender shoots grow to 2-3 cm (different host plants have differences, such as large leaf plants of citrus reticulata, and the like grow to tender shoots of about 3cm, and small leaf plants of Murraya paniculata, and the like grow to tender shoots of about 2 cm); selecting a certain number of the citrus psyllids prepared in the step (1) to be connected into a feeding cage, and connecting 2-3 citrus psyllids to each tender tip; after the diaphorina citri is inoculated for 2-4 days (the total time is not more than 4 days), taking out all adults, and supplementing the diaphorina citri according to spawning conditions in the period; after about 10 days (slightly varying depending on the temperature etc.) from the time of the inoculation of the adult diaphorina citri, the eggs develop to a suitable stage and can be inoculated with parasitic wasps.
(3) Inoculating parasitic wasps:
if the wood louse ages are inconsistent, when 30% of 3-year-old wood louse nymphs appear in the cage, the method is suitable for being connected with the dorifer flea bees; if the ages are consistent, parasitic bees can be accessed when 3-year-old nymphs just appear; the prepared adult dorifer of the Alhagia lupulus in the step (1) is collected and is put into a raising cage, the input quantity is determined according to the actual quantity of the diaphorina citri, and female bees are generally the case: the quantitative ratio of diaphorina citri nymphs is between 1:15 to 1: 30. The recorded Apis cerana Fabricius flea swarm can parthenogenesis in China at present, and male insects are not needed.
And (3) continuing to feed, wherein the eclosion of the non-parasitized diaphorina citri precedes the eclosion of the parasitic wasps, and the newly eclosion diaphorina citri adults are removed in time before the eclosion of the parasitic wasps.
(4) Collecting parasitic wasps:
at the outer part, the parasitic wasp larvae quickly start pupating in the diaphorina citri nymphs. Starting from the time of inserting the adult parasitic wasps, new bees are eclosion continuously from 13 th to 15 th days, and the period lasts for about 7 days, and bees can be collected continuously.
The plant can be collected in the pupa period or the adult period, so as to be used for indoor experiments, manual release, biological control or population supplement in the step (1), and the like.
After the bee harvesting is finished, the host plant can be reused. If the diaphorina citri in the step (3) is collected timely, the host plant is in a healthy and pest-free state after the parasitic wasps emerge, and the parasitic wasps can be directly germinated again for the next period of propagation, but the parasitic wasps are continuously used for no more than 3 times.
Preferably, in the step (1), an insect-proof net with more than 50 meshes (the aperture is less than 0.28 mm) is adopted to isolate the population. The feeding condition is controlled to be 26 ℃ in temperature and 50-60% in humidity, and the photoperiod L: d=16: 8, the illumination intensity of the middle part of the plant is 4500lx. The gardening nutrient soil is selected, water is irrigated for 1 time every 3 days, and timely fertilization is conducted according to the dosage of the commercial compound fertilizer. Pruning the branches of the host plants, enabling the tops of the plants to be about 10cm away from the light source, preventing light blocking and facilitating management of diaphorina citri and parasitic wasps. In addition, about the same number of host plants are prepared and placed outdoors to the sun, normal management is carried out, insect-proof treatment is carried out, half of the plants are replaced every 3 months, or the plants with serious problems are replaced at any time.
Preferably, the raising space in the step (2) is isolated from other areas by an insect-proof net with a pore size of more than 50 meshes (pore size <0.28 mm). 2 pots of host plants can be placed in the rearing cage, and the distance between the top surface and the plants is 15cm. The illumination intensity in the middle of the plants in the cages was about 4500lx. Suitable periods of shoot growth for different host plants differ, for example, about 3cm for large leaf plants such as citrus reticulata and about 2cm for small leaf plants such as Murraya koenigii. The inoculated psyllids were selected from individuals with an enlarged abdomen and an orange color, 2 heads per tender tip, and the total time of inoculation was 2 days.
Preferably, in the step (3), the newly emerged diaphorina citri adults are removed from the rearing cages every day after 15 days, and after about one week, no diaphorina citri will emerge, and the operation can be stopped. Increasing the ratio of the bees (such as 1:20, 1:15, etc.) can reduce the workload of post-treatment of psyllids (according to the actual number of psyllids, the psyllids can be collected every other day or even once every other day).
Preferably, in the step (4), the peak period of emergence of the dorifer flea bees is basically 3 days after the emergence, and the bees can be collected intensively for 2 times on the 3 rd day and the 7 th day. When collecting bees, the raising cage is covered by the shading cloth, and only the top collecting mouth is illuminated, so that a proper container can be connected, and parasitic bees can climb into the raising cage by themselves when the parasitic bees tend to light. The plants after the bee harvest are placed at the position with sufficient illumination for cultivation and are reused at intervals of at least 1 breeding period.
The invention is further illustrated by the following examples.
The following 2 examples share the same doripes and the conditions are described herein in detail: the Alhagia lupeus population is arranged in an indoor closed partition area, the commercial Murraya koenigii is adopted as a host plant, a pot plant is placed on an illumination culture rack, and soil is commercial gardening nutrient soil. The diaphorina citri and the diaphorina citri flea bee population are collected from Guangzhou city and the surrounding area, and the indoor population is maintained for more than 2 years. The temperature is set to 26 ℃, the humidity is 50-60%, and the photoperiod is L: d=16: 8, the illumination intensity is 4500lx. Water was poured 2 times per week to keep the soil moist. And (5) timely trimming and removing the disease plants, properly controlling the quantity of the diaphorina citri, and preventing outbreaks. Controlling the population number of indoor non-target organisms (whiteflies, mites, spiders, etc.).
The specifications of the feeding cages in the steps (2), (3) and (4) are unified to be 75cm long, 60cm wide and 90cm high. The room temperature is 28 ℃, the humidity is 40-60%, and the photoperiod is L: d=16: 8, the illumination intensity in the cage is 3000lux, each plant is watered 2 times per week, and 500mL each time.
Example 1:
in the embodiment, 1 feeder cage is arranged, 1 strain of citrus reticulata is placed in the feeder cage, and the pot and the soil are taken when purchased and are not replaced. One branch growing from the basal part of the stem is trimmed to form a leaf and a bud, the leaves of the rest branches are all trimmed, about 50 tender buds are left in total, and the width of the final plant is about 30cm and the height is about 85cm.
When the length of the tender bud is about 3cm, 100 citrus psyllids are inoculated, and 50 nodes are respectively planted. After 72 hours (3 days) the diaphorina citri was removed leaving only newly laid eggs on the plants. The date of the access to the diaphorina citri was marked as day 1, and the development of diaphorina citri nymphs on the buds was observed daily from day 7.
The diaphorina citri nymphs had entered 3 years and were more consistent in age on day 12. Based on the estimation, the number of nymphs was about 150. According to the female bees: diaphorina citri nymph = 1:30 (quantity ratio) 5 females of the Alhagia arguta are inoculated.
11 newly emerged diaphorina citri were observed in the cages on day 18, but the first emergence time should be on day 16 or 17. Newly emerged citrus psyllids were counted and removed daily thereafter. No diaphorina citri was allowed to eclosion by day 25.
Day 22 (10 days after the parasitic wasps are accessed) was started to record the emergence of the parasitic wasps on a daily basis for at least 7 consecutive days. FIG. 1 is a graph showing the statistical result of daily emergence of diaphorina citri and diaphorina citri flea bees in a feeder cage.
As shown in fig. 1, finally, under the production conditions of this example, the diaphorina citri 106 heads were co-feathered throughout the entire propagation cycle, and the apricots 105 heads were obtained. Judging according to the method, the actual number of the nymphs of the connected bees Shi Mushi is more than 200, and the female bees: the actual number proportion of the diaphorina citri nymphs is 1: about 40, the parasitic wasp access yield ratio is 21. Because the parasitic wasp has a low access proportion, the control effect on the diaphorina citri is general, and the parasitic rate is about 50%.
Example 2:
in the embodiment, 1 feeder cage is arranged, 1 strain of citrus reticulata is placed in the feeder cage, and the pot and the soil are taken when purchased and are not replaced. The whole plant is trimmed to form a leaf and a bud, about 50 tender buds are reserved, and the width of the final plant is about 30cm and the height is about 85cm.
When the length of the tender bud is about 3cm, 100 citrus psyllids are inoculated, and 50 nodes are respectively planted. After 72 hours (3 days) the diaphorina citri was removed leaving only newly laid eggs on the plants. The date of the access to the diaphorina citri was marked as day 1, and the development of diaphorina citri nymphs on the buds was observed daily from day 7.
The most diaphorina citri nymphs have entered age 2 on day 11, at which time the apricots are vaccinated. About 120 citrus psyllid nymphs, according to the female bees: diaphorina citri nymph = 1:30 (quantity ratio) 4 heads of the Apis cerana Fabricius are inoculated.
2 newly emerged diaphorina citri were observed in the cages on day 18, after which the newly emerged diaphorina citri was counted and removed daily. No diaphorina citri feathered from day 23.
Day 22 (10 days after the parasitic wasps are accessed) was started and the parasitic wasps emergence was recorded for at least 7 consecutive days. FIG. 2 is a graph showing the statistical result of daily emergence of diaphorina citri and diaphorina citri flea bees in a feeder cage.
As shown in fig. 2, finally, under the production conditions of this example, 24 heads of diaphorina citri co-eclosion were co-eclosion during the whole propagation cycle, and 83 heads of apricots were obtained. And judging that the number of diaphorina citri nymphs is more than 100 when the bees are connected. Female bees: the actual number proportion of the diaphorina citri nymphs is 1: about 25, the parasitic wasp access yield ratio is 21. Under the condition of higher bee-receiving proportion, the method has obvious population pressing effect on the diaphorina citri nymphs, and the parasitic rate is about 78%. Considering that the parasitic wasp adults can also feed on diaphorina citri nymphs, the actual control effect should be greater than 80%.
The method can quickly, regularly and quantitatively obtain the dorifer flea pupa and the adult in any time period throughout the year, can adjust the raising scale at any time according to the expected propagation target, and has high activity of the obtained parasitic wasps, and good control effect on the diaphorina citri under the condition of properly improving the bee receiving proportion.
The foregoing is merely a preferred embodiment of the present invention, and it should be noted that modifications and substitutions can be made by those skilled in the art without departing from the technical principles of the present invention, and these modifications and substitutions should also be considered as being within the scope of the present invention.
Claims (6)
1. The large-scale production method of the dormitory-lice flea bees is characterized by comprising the following steps of:
(1) Establishing an alishi lutiao population: the method comprises the steps of (1) raising diaphorina citri host plants, diaphorina citri and diaphorina citri flea bees in a relatively isolated environment to form a mixed population, ensuring stable raising conditions, ensuring stable growth vigor of the diaphorina citri host plants, and maintaining a sufficient number of parasitic bees;
(2) Preparing a diaphorina citri to be parasitized population: placing healthy host plants in a rearing cage, and promoting tender shoots to occur through pruning and water and fertilizer control; when the tender buds of the host plants grow to be suitable for spawning of the diaphorina citri, selecting the diaphorina citri adults in the step (1), and taking out all adults after the diaphorina citri spawns on tender tips for 2-4 days; continuously breeding diaphorina citri eggs in the cages, and inoculating parasitic wasps when the eggs develop to a proper stage;
(3) Inoculating parasitic wasps: collecting the adult dorifer flea beetles in the step (1), feeding the adult dorifer flea beetles into the prepared diaphorina citri to be parasitized population, and timely removing newly emerged diaphorina citri adults during feeding;
(4) Collecting parasitic wasps: at the outer part, the parasitic wasp larva starts pupating in the diaphorina citri nymph body soon, and new bees emerge continuously from 13 to 15 days after the parasitic wasp adults are inoculated, and the period lasts for about 7 days, and bees can be collected continuously;
in the step (2), the number of the access of the adult diaphorina citri is 2-3 per young shoot; the suitable stages are: when the nymphs of the diaphorina citri are consistent in age, the 3-age nymphs are the proper stage; when the nymphs of the diaphorina citri are inconsistent in age, the 3-year nymphs are at a proper stage when the ratio is more than 30%;
in the step (1), stable feeding conditions are ensured, the growth vigor of the diaphorina citri host plant is stable, and the parasitic wasps with sufficient quantity are maintained specifically comprising: 1) Controlling the ambient temperature to be 25-30 ℃, the humidity to be 45-75%, the illumination time to be 12-16 hours, and the illumination intensity in the middle of the plant to be more than 3000lx; 2) Controlling water and fertilizer management, ensuring good growth vigor of host plants and stable tender shoot occurrence quantity; 3) Controlling the population number of non-target organisms; 4) Collecting the fowls in the field and supplementing the population quantity;
in the step (3), the number of the access of the ari food lutiao adult bees is as follows: the number ratio of the female Alhagia arguta adults to the diaphorina citri is 1:15-30, the breeding is continued, the diaphorina citri which is not parasitized in the period is eclosion before the parasitic wasps, and the newly eclosion diaphorina citri adults are removed in time before the parasitic wasps eclosion;
in the step (2), six sides of the raising cage are covered with a net, the light transmittance meets the condition that the illumination intensity of the middle part of the plant is larger than 3000lx, the mesh number of the net is larger than 50 meshes, the aperture is smaller than 0.28mm, 1 opening for daily management is arranged on the side surface, and 2 openings for collecting the adult Apis cerana Fabricius are arranged on the top.
2. The method for mass production of the apricots as claimed in claim 1, wherein in the step (4), the host plant is reused after the parasitic wasps are collected.
3. The method for mass production of dorifer solepoxella according to claim 2, wherein said host plant is continuously reused no more than 3 times.
4. The method for mass production of the apricots according to claim 1, wherein the non-target organisms include mites and germs.
5. The method for mass production of the apricots as claimed in claim 1, wherein in the step (1), the relatively isolated environment means a separate feeding area.
6. The method for mass production of the apricots according to claim 1, wherein in the step (2), the plant water and fertilizer control comprises: and the concentrated emergence of new buds is promoted by pruning, watering and fertilizing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110954504.7A CN113558014B (en) | 2021-08-19 | 2021-08-19 | Large-scale production method of dormitory lice flea bees |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110954504.7A CN113558014B (en) | 2021-08-19 | 2021-08-19 | Large-scale production method of dormitory lice flea bees |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113558014A CN113558014A (en) | 2021-10-29 |
| CN113558014B true CN113558014B (en) | 2023-06-30 |
Family
ID=78172096
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110954504.7A Active CN113558014B (en) | 2021-08-19 | 2021-08-19 | Large-scale production method of dormitory lice flea bees |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113558014B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114600840B (en) * | 2022-02-25 | 2023-04-07 | 广西特色作物研究院 | Method for determining biological activity of diaphorina citri nymphs |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104621052A (en) * | 2015-02-11 | 2015-05-20 | 广东省昆虫研究所 | Diaphorina citri Kuwayama parasitism natural energy scale breeding method |
| CN106358877A (en) * | 2016-09-13 | 2017-02-01 | 广东省生物资源应用研究所 | Application of Pyemotes zhonghuajia in preparing preparation for biological prevention and control of diaphorina citri |
| CN106954592B (en) * | 2017-04-14 | 2020-08-07 | 华南农业大学 | Large-scale breeding method for Alliid lice spikemoss |
-
2021
- 2021-08-19 CN CN202110954504.7A patent/CN113558014B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN113558014A (en) | 2021-10-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103385220B (en) | Scale breeding method for encarsia formosa | |
| CN101095396A (en) | Technique of planting green vegetables | |
| CN108967070B (en) | Method for planting passion fruit by soil renovation in next year | |
| CN102440165A (en) | Method of cultivating amygdalus persica A.N. trees | |
| CN106954592B (en) | Large-scale breeding method for Alliid lice spikemoss | |
| CN103210848A (en) | Broccoli lateral branch cultivating plant and hybrid seed production method | |
| CN105210768A (en) | The cultivation method of one planting fruit-trees garden interplanting medicine vegetables | |
| CN103798049A (en) | Lonicera japonica twig cutting cultivation method | |
| CN110547158A (en) | Strawberry field cultivation method | |
| CN109757274B (en) | High-yield and high-efficiency transplanting method for momordica grosvenori tissue culture seedlings | |
| CN111466263A (en) | Grafting seedling method for pepper cultivation in sunlight greenhouse | |
| CN105165313A (en) | Method for breeding aphidius gifuensis within short cycle | |
| CN114175977A (en) | Cultivation method of tomatoes in sunlight greenhouse | |
| CN113558014B (en) | Large-scale production method of dormitory lice flea bees | |
| CN112021168A (en) | Method for planting dendrobium officinale by using straw ropes to simulate wild trees | |
| CN109744047A (en) | A kind of implantation methods of mid-autumn crisp jujube | |
| CN104303765B (en) | The high-yield planting method of the stem of noble dendrobium | |
| CN104160856B (en) | A kind of cultivation method improving kale cut-flower amount and production of hybrid seeds amount | |
| CN114158365B (en) | Method for cultivating big seedlings of camellia chrysantha | |
| CN105145269A (en) | Efficient seedling cultivating method for leafbuds of Yunshan 2# grape novel variety | |
| CN105475232B (en) | Method for large-scale feeding and aphid control of aphidiidae | |
| CN108260487A (en) | A kind of interior white potato seedling seeding cultivating method | |
| CN113951023A (en) | Method for preventing and treating mango thrips by utilizing orius minutus | |
| CN106489477A (en) | A kind of Ecology Chinese podophyllum root method for culturing seedlings | |
| CN107258304B (en) | Cultivation method for crop rotation of eggplants and rice in winter and spring |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |