CN103503836A - Plutella xylostella cotesia rubecula artificial propagation production method - Google Patents
Plutella xylostella cotesia rubecula artificial propagation production method Download PDFInfo
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Abstract
The invention discloses a plutella xylostella cotesia rubecula artificial propagation production method which comprises the following steps: collecting and propagating plutella xylostella cotesia rubecula species, producing hosts, conducting inoculation, propagation, development and feeding on plutella xylostella, conducting inoculation and cultivation on plutella xylostella cotesia rubecula, separating the plutella xylostella cotesia rubecula from the plutella xylostella, and conducting collecting, reserving and seed stock on plutella xylostella cotesia rubecula pupae. By means of the scheme of the plutella xylostella cotesia rubecula artificial propagation production method, advantaged plutella xylostella cotesia rubecula specie selection, propagation and pasturing technology is completed, high-quality, efficient and mass plutella xylostella cotesia rubecula propagation is achieved, mass production can be carried out, the plutella xylostella cotesia rubecula artificial propagation production method is applied to the technical field of biological control over agricultural pests, the plutella xylostella cotesia rubecula artificial propagation production method is used for preventing and treating primary pests such as the plutella xylostella on brassicaceous vegetables and meets production requirements of organic vegetables and green vegetables.
Description
Technical field
The present invention relates to the breeding production technology of a kind of honeybee, particularly relate to a kind of Cotesia plutellae larvae artificial propagation production method, belong to the biological prevention field of pest.
Background technology
Diamond-back moth Plutella xylostella (Linnaeus) is the important pests on brassicaceous vegetable, and diamond-back moth is caused great economic loss every year all over the world, and the loss that the whole world causes because of diamond-back moth every year and expenses for prevention and control are over 4,000,000,000 dollars; China in the last few years, diamond-back moth have an interesting gesture increasing the weight of year by year, and pesticide resistance is more and more stronger, the production of rampant harm China brassicaceous vegetable; Because diamond-back moth most time is the back side at plant leaf, control is difficulty comparatively, causes and causes repeatedly medication, the persticide residue severe overweight in the vegetables that result makes to produce; For many years, approach and the effective ways of biological control are all being sought in countries in the world.Parasitic wasp is the Important Natural Enemy resource of diamond-back moth, utilizes the existing successful example of parasitic wasp control diamond-back moth and relevant report.Cotesia plutellae larvae Cotesia plutellae (Kurdjumov) is the main parasitic natural enemy of diamond-back moth, and natural enemy sociales.Becoming at present for preventing and treating one of reasonable parasitic wasp of diamond-back moth, is a kind of diamond-back moth parasitic wasp that gets a good eye future.In China, existing investigation shows, Cotesia plutellae larvae all has natural distribution and overwintering situation in northern China and south; And indivedual places or the sociales parasitic wasp of diamond-back moth, also there will be sometimes higher parasitic rate; But there is not yet at home the research report that it is carried out to biological control and the application of large area field and control efficiency aspect.
The present patent application people has done more intensive research to Cotesia plutellae larvae, Cotesia plutellae larvae is China's diamond-back moth Dominant Natural Enemies distributed more widely, relevant research shows that it has good Natural control action to diamond-back moth, Cotesia plutellae larvae can all larval stage of parasitic diamond-back moth and is completed growth, but the larva with parasitic diamond-back moth 2-3 age is the most suitable, and parasitic rate is also the highest; The larva in 1 age and 4 ages is unwell to parasitism, and rate of successful parasitism is also relatively low.Because the identical of the parasitic length of time of suitable diamond-back moth and Cotesia plutellae larvae emergence period is parasitic successfully crucial, China there is no the report of ripe a large amount of artificial propagation techniques at present.
Summary of the invention
In view of above-mentioned, by a large amount of research and long-term groping, the resource investigation and the carrying out of Biological Control of Plutella xylostella that the invention provides a kind of Cotesia plutellae larvae artificial propagation production method ，Wei China diamond-back moth parasitic wasp have been established good basis.
Concrete Reproduction method is as described below:
1. honeybee kind gathers and breeding:
1) honeybee kind gathers: diamond-back moth, occur on serious field plant blade, gather the white cocoon of Cotesia plutellae larvae, i.e. the pupa of braconid wasp;
2) honeybee kind is purified and is selected excellent: the pupa of the above-mentioned braconid wasp collecting is placed under 25 ℃ of left and right, RH.60-70% condition and cultivates and grow, sprout wings and after honeybee, be transferred to immediately in diamondback moth larvae body, then by emergence bee hatching rate, parasitic rate, fertility, male and female are compared, storage characteristic and the search capability of target pest is detected to test, select good honeybee kind;
3) honeybee kind expands numerous: the good honeybee kind choosing is expanded numerous, the Cotesia plutellae larvae honeybee kind quantity of accumulation sufficient amount, is used during in order to a large amount of numerous honeybee;
4) preservation of honeybee kind: Cotesia plutellae larvae is grown while cocooing the phase, should gather immediately, and carries out alternating temperature processing, after cooling step by step, is stored under 8-10 ℃, RH.50-60% condition and preserves;
2. host's production:
Produce routinely that cultivation method is sowed, grown seedlings, transplanting and field planting host plant for rearing plant, sufficient in illumination, in the clean room under 20-28 ℃ of temperature match curing conditions, cultivate host plant for rearing plant;
When host plant grows to 5-6 sheet true leaf, Plant colonization is in the flowerpot of diameter 18cm left and right;
Every 10 days a collection of host plants of field planting, every batch of field planting 72 strains; Standby during 8-9 sheet true leaf;
3. the inoculation reproduction of diamond-back moth:
1) preparation in diamond-back moth worm source: before starting numerous honeybee 3 weeks, by clean cabbage seedling 18 basins of 8-9 sheet true leaf, put into diamond-back moth receptacle, inoculation diamond-back moth adult 2000-3500 head, standby when filial generation diamond-back moth adult sprouts wings in a large number;
2) when filial generation diamond-back moth adult sprouts wings in a large number, move into 72 basins cabbage seedling to be inoculated, inoculation 24-26 hour, during inoculation, need often shake the plant as worm source, also will rock new talent 3-4 time gently, it is laid eggs evenly simultaneously, when general each vacuum side of blade has ovum more than 150, can finish inoculation.
3) after inoculation has been laid eggs, with gobo, cover 4 of dependent insect cage (left and right, upper, below), utilize diamond-back moth adult phototaxis principle, shake the host plant of Oviposition of Diamondback Moth in shrouding, drive diamond-back moth adult on host plant away;
4) then, 66 basins are wherein taken out of, move into diamondback moth larvae and cultivate growth chamber, cultivate and grow;
5) leave 6 basin plant, for changing the old plant as worm source, change weekly 6 basins, within 3 weeks, changed; During replacing, old plant is pulled up, after diamond-back moth adult all sprouts wings, then clear up away;
(4) diamondback moth larvae is grown:
After inoculation has been laid eggs, with the host plant of Eggs of Diamondback Moth, move into diamondback moth larvae and cultivate growth chamber, and strengthen management, promote leaf growth, standby when diamondback moth larvae is grown to 2 age;
(5) Cotesia plutellae larvae inoculation:
By the host plant with 2-3 diamondback moth larvae in age, be moved into a large amount of Cotesia plutellae larvaes and become the interior inoculation of shrouding of honeybee, there to be Cotesia plutellae larvae 4-6 head to be advisable on every cabbage leaves; After 24-26 hour, utilize Cotesia plutellae larvae to become honeybee phototaxis principle, make seedling and honeybee separately.
(6) Cotesia plutellae larvae is cultivated:
When being grown by 7 days by parasitic diamondback moth larvae, there is Cotesia plutellae larvae mature larva to start to get out in diamondback moth larvae body, within 1-2 hour, can form the cocoon of pupating of white; Answer timely collecting, packing or refrigerate standby.
(7) bee pupa is collected and application:
At Cotesia plutellae larvae mature larva, cocoon the initial stage, should collect in time, store, for dispensing or directly field application.Suitably moisturizing of lay up period, to keep the vigor of Cotesia plutellae larvae, be generally advisable with interior at 20 days storage period.
(8) reserve seed for planting: when harvesting of cocoon is collected, choose and grow healthyly, individuality is larger, the glossiness Cotesia plutellae larvae cocoon of cocoon shell is as honeybee kind, the storage respectively that different batches is gathered, and the Cotesia plutellae larvae inoculation while being ready for use on next breeding is used.
Wherein the inoculation reproduction of the described diamond-back moth of step (3), utilizes diamond-back moth adult phototaxis principle after inoculation, shakes the host plant of Oviposition of Diamondback Moth in shrouding, and drives diamond-back moth adult away.
The wherein described Cotesia plutellae larvae inoculation of step (5), must guarantee in the time of inoculation that the emergence period of Cotesia plutellae larvae and diamondback moth larvae 2-3 match the length of time.
Wherein the described Cotesia plutellae larvae of step (5) is inoculated, and meets the honeybee worm ratio 1:8-12 of honeybee.
The wherein described Cotesia plutellae larvae inoculation of step (5), the Cotesia plutellae larvae male and female of inoculation are than being 6-8:1.
The wherein collection of the described Cotesia plutellae larvae cocoon of step (7), the male and female ratio of take when dispensing or field application is advisable as 6-8:1 ratio.
Cotesia plutellae larvae artificial propagation production method of the present invention, tool has the following advantages:
1. adopt technical scheme provided by the invention perfect advantage honeybee kind select, numerous bee technology has been realized high-quality, has been bred Cotesia plutellae larvae efficiently, in enormous quantities;
2. production method of the present invention can be carried out large-scale continuous production, be applied to the biological prevention field of pest, be used for preventing and treating diamond-back moth insect, thereby meet to a greater extent the needs of agricultural production, for the biological control of Cruciferous Vegetable Pests provides convenience;
3. production method of the present invention has been done special control to the cultivation of Cotesia plutellae larvae, storage, thus reasonable inoculation and the utilization of more convenient parasitic wasp;
4. production method of the present invention has been done requirement to the inoculative proportion of Cotesia plutellae larvae, diamond-back moth, thereby has improved the parasitic rate of parasitic wasp;
5. in production seeded process of the present invention, make the emergence period of Cotesia plutellae larvae and diamondback moth larvae 2-3 match the length of time as far as possible, for the parasitism of parasitic wasp, laying eggs provides more multimachine meeting, thereby improved egg laying amount and parasitic rate;
6. Cotesia plutellae larvae of the present invention is the natural advantage natural enemy of diamond-back moth, and diamond-back moth is had to good Natural control action;
7. production method of the present invention has reference role to the breeding of other parasitic wasp, storage and release;
8. the present invention is that fine basis has been established in the breeding utilization of China diamond-back moth parasitic wasp.
Accompanying drawing explanation
Fig. 1 is Cotesia plutellae larvae artificial propagation production method process flow diagram.
Embodiment
cotesia plutellae larvae large scale artificial breeding production process mainly as shown in Figure 1, specific as follows described in:
1. gather and breeding Cotesia plutellae larvae honeybee kind:
1) field bee kind: occur on serious field plant blade diamond-back moth, gather the white cocoon of Cotesia plutellae larvae, i.e. the pupa of braconid wasp;
2) purification of honeybee kind and preferred: the pupa of the above-mentioned braconid wasp collecting is placed under 25 ℃ of left and right, RH.60-70% condition and cultivates and grow, sprout wings and after honeybee, be transferred to immediately in diamondback moth larvae body, then by emergence bee hatching rate, parasitic rate, fertility, male and female are compared, storage characteristic and the search capability of target pest is detected to test, select good honeybee kind;
3) honeybee kind expands numerous: the good honeybee kind choosing is expanded numerous, the Cotesia plutellae larvae honeybee kind quantity of accumulation sufficient amount, is used during in order to a large amount of numerous honeybee;
4) preservation of honeybee kind: Cotesia plutellae larvae is grown while cocooing the phase, should gather immediately, and carries out alternating temperature processing, after cooling step by step, is stored under 8-10 ℃, RH.50-60% condition and preserves;
2. host's production:
Host plant should select wild cabbage, cauliflower class diamond-back moth to like edible plant, and the present invention only be take wild cabbage and described as example, and host insect is diamond-back moth.
Produce routinely that cultivation method is sowed, grown seedlings, transplanting and field planting host plant for rearing plant wild cabbage, sufficient in illumination, in clean room under 20-28 ℃ of temperature match curing conditions, cultivate host plant for rearing plant cabbage seedling, when cabbage seedling grows to 35-40 days (during 5-6 sheet true leaf), in the flowerpot by its individual plant transplant planting in about diameter 18cm;
Every 10 days a collection of host plants of field planting, every batch of field planting 72 strain left and right; When 45-50 days (8-9 sheet true leaf), can be moved into inoculation diamond-back moth in Oviposition of Diamondback Moth chamber (cage).
3. the inoculation reproduction of diamond-back moth:
1) preparation in diamond-back moth worm source: before starting numerous honeybee 3 weeks, by clean cabbage seedling 18 basins of 8-9 sheet true leaf, put into diamond-back moth receptacle, inoculation diamond-back moth adult 2000-3500 head, standby when filial generation diamond-back moth adult sprouts wings in a large number;
2) when filial generation diamond-back moth adult sprouts wings in a large number, move into 72 basins cabbage seedling to be inoculated, inoculation 20-24 hour, during inoculation, need often shake the plant as worm source, also will rock new talent 3-4 time gently, it is laid eggs evenly simultaneously, when general each vacuum side of blade has ovum more than 150, can finish inoculation.
3) after inoculation has been laid eggs, with gobo, cover 4 of dependent insect cage (left and right, upper, below), utilize diamond-back moth adult phototaxis principle, shake the host plant of Oviposition of Diamondback Moth in shrouding, drive diamond-back moth adult on host plant away;
4) then, 66 basins are wherein taken out of, move into diamondback moth larvae and cultivate growth chamber, cultivate and grow;
5) leave 6 basin plant, for changing the old plant as worm source, change weekly 6 basins, within 3 weeks, changed; During replacing, old plant is pulled up, after diamond-back moth adult all sprouts wings, then clear up away;
Special instruction: step (3) and step (5) operate period simultaneously, and, when taking out of 66 basins, 6 basins that stay are also changed, and the old plant of 6 basin swaps out.
4. diamondback moth larvae is grown:
Grow chamber and generally prepare 6-8 square metre; After inoculation has been laid eggs, with the host plant of Eggs of Diamondback Moth, move into diamondback moth larvae and cultivate growth chamber, cut off growing point, and strengthen management, promote leaf growth, standby when diamondback moth larvae is grown to 2 age, for inoculating Cotesia plutellae larvae;
5. Cotesia plutellae larvae inoculation:
Cotesia plutellae larvae inoculated and cultured chamber, generally prepares 6-8 square metre.Host plant 66 basins with 2-3 diamondback moth larvae in age of above-mentioned cultivation are moved into, be moved into a large amount of (ten thousand of 0.5-1.0) Cotesia plutellae larvaes and become the interior inoculation of shrouding of honeybee; This operation is to become honeybee to be inoculated on the host plant with 2-3 diamondback moth larvae in age of above-mentioned cultivation Cotesia plutellae larvae; There to be Cotesia plutellae larvae 4-6 head on every cabbage leaves, be advisable; After 24-26 hour, utilize Cotesia plutellae larvae to become honeybee phototaxis principle, make seedling and honeybee separately.Meet the honeybee worm ratio 1:8-12 of honeybee, in the time of inoculation, must guarantee that the emergence period of Cotesia plutellae larvae and diamondback moth larvae 2-3 match the length of time; Guaranteeing that both coincide substantially adopts conventional insect raising measure, as control indoor temperature, or suitably adjusts feeding time etc. according to both physiological property and law of development separately, thereby regulates both development progresses; Meanwhile, preferably guarantee that Cotesia plutellae larvae and the honeybee worm ratio of diamond-back moth are 1:8-12.
6. Cotesia plutellae larvae is cultivated:
When being grown by 7 days by parasitic diamondback moth larvae, should be specifically noted that have Cotesia plutellae larvae mature larva to start to get out in diamondback moth larvae body, within 1-2 hour, can form the cocoon of pupating of white; Answer timely collecting, packing or refrigerate standby.
7. bee pupa is collected and application:
At Cotesia plutellae larvae mature larva, cocoon the initial stage, should collect in time, carry out alternating temperature processing, storage, for dispensing or directly field application;
1) timely collecting, and carry out alternating temperature processing is stored under 8-10 ℃, RH.50-60% condition and preserves after cooling step by step; Also be to guarantee the consistent basic premise of development progress of future generation.
2) be difficult for storage period oversize: according to experimental observation, find, along with the prolongation of storage period, the eclosion rate of Cotesia plutellae larvae can reduce gradually along with the prolongation of storage period; Generally at 20 days, with interior, be advisable.
3) suitably moisturizing of lay up period, to keep the vigor of Cotesia plutellae larvae.
4) application prerequisite honeybee, also should be after cascade raising temperature, then is put under natural temperature, in order to avoid suddenly heat up, affects the vigor of Cotesia plutellae larvae.
8. reserve seed for planting: when harvesting of cocoon is collected, choose and grow healthyly, individuality is larger, the glossiness Cotesia plutellae larvae cocoon of cocoon shell is as honeybee kind, the storage respectively that different batches is gathered, and the Cotesia plutellae larvae inoculation while being ready for use on next breeding is used.
Embodiment bis-: comparative trial
Comparative trial 1: the screening of host plant
1. materials and methods
1) for examination insect
Diamond-back moth ，Zhe chamber is raised many generation tests and is used diamond-back moth.
Cotesia plutellae larvae, picks up from the court's cabbage field in growth later stage.
2) host plant
Take wild cabbage, cauliflower, Chinese cabbage, rape as supplying examination host.
The seed of wild cabbage, cauliflower, Chinese cabbage, rape is all purchased from vegetables institute's special vegetable center, Beijing City Agriculture and Forestry Institute.
3) experimental condition and equipment
Test is carried out indoor, and temperature is controlled at 25-27 ℃, relative moisture 55-75%; Dependent insect cage is aluminum alloy frame, and specification is 54 * 45 * 40cm; Wherein one side is glass sliding door, and bottom surface is glass, and all the other 4 envelopes are with 120 order nylon mesh; Host plant is planted in plastic seeding culturing alms bowl, and cultivation matrix is plow layer soil/turfy soil/vermiculite (respectively account for 1/3 ratio, sterilization after mixing, re-uses).
4) test method
Test is artificial culture in laboratory with host plant.By the pot for growing seedlings of having sowed water permeable after, be placed in clean dependent insect cage, in every cage, put a kind of host plant, cultivate without the clean seedling of worm.When plant to be planted grows into 8-9 sheet leaf, the new diamond-back moth adult sprouting wings of access 40-60 head in every cage; After 7-8 days, the plant leaf back side will have the diamondback moth larvae in each length of time to occur, at this moment in every cage, the new emergence Cotesia plutellae larvae of access 60-80 head left and right becomes honeybee, guarantees that female drone all has; Strengthen the water and fertilizer management of host plant, give sufficient illumination, guarantee that its growth conditions is sufficient; If find that there is other sick worm harm, should process immediately or remove, prevent from jeopardizing whole population after diffusion.
Duration of test is wanted the situation of breeding of observed and recorded host plant growing state and diamond-back moth and Cotesia plutellae larvae, evaluates the quality of each host plant; Filter out the host plant that is not only easy to cultivation management but also can be jointly liked by diamond-back moth and Cotesia plutellae larvae.
5) host plant evaluation index
A: the length of growth cycle.Comprising can be for the time that can survive time of testing and inoculation from being seeded into.For example: after rape seed, emerge and grow all very fast, after one week, there is 2-3 sheet true leaf to occur, about 12 days, can be used for having tested, but it is too short to connect the time that can maintain after worm, cannot complete whole experiment, even in the larvae development of diamond-back moth to being applicable to connecing before honeybee, plant has just decayed, and cannot continue experiment.Wild cabbage, cauliflower are just in time contrary, can maintain for a long time after connecing worm, and can normal growth, and can resist well the infringement of other insect, but from after planting at least needing 35-40 days to the time that can be used in experiment, and seedling stage also manageability not, relative death rate is also higher.
B: the level and smooth degree of vacuum side of blade.Vacuum side of blade how many direct activities that affect diamond-back moth and Cotesia plutellae larvae of raw burr, and parasitic rate is impacted, and is also unfavorable for various observation work simultaneously.For example Chinese cabbage vacuum side of blade burr is intensive and long, affects the parasitic rate of Cotesia plutellae larvae; Wild cabbage, cauliflower are just in time contrary, and vacuum side of blade, smoothly without burr, can not affect the movable and parasitic of examination worm.
C: examination worm fancy grade.Physical factor and other unknown factor of comprising above B item, make to try worm host plant shown to obvious selectivity.For example: diamond-back moth is relatively liked wild cabbage, cauliflower, rape, Cotesia plutellae larvae is relatively liked rape, wild cabbage, cauliflower.
By each host plant of overall merit, raise the quality of diamond-back moth and Cotesia plutellae larvae, filter out and be not only easy to the host plant that diamond-back moth grows but also can be liked by Cotesia plutellae larvae.
2. results and analysis
The pluses and minuses of each host plant are as shown in table 1 below.By reality, cultivate, connect worm, release, comprehensively more every kind of host plant, has finally selected wild cabbage as the host plant that keeps population and test; Compare with other 3 Plants, wild cabbage is the same with cauliflower plantation, and diamond-back moth and Cotesia plutellae larvae are all liked, and do not affect normally carrying out of test.
The odds of four kinds of host plants raising diamond-back moths of table 1 and Cotesia plutellae larvae
| Host plant | Advantage | Shortcoming |
| Chinese cabbage | Growth cycle is short, and blade is large | Blade face burr is intensive and long, high humidity |
| Rape | Growth cycle is short, and blade is level and smooth | Total amount is little, is very easily eaten up |
| Wild cabbage | Blade is level and smooth, host parasitic wasp hobby | Poor growth, seedling stage is manageability not |
| Cauliflower | Blade is level and smooth, host parasitic wasp hobby | Poor growth, seedling stage is manageability not |
Comparative trial 2: the research that different host plants grows to Cotesia plutellae larvae
1. materials and methods
1.1 for examination insect
Diamond-back moth ，Zhe chamber is raised many generation tests and is used diamond-back moth.
Cotesia plutellae larvae, picks up from the court's cabbage field in growth later stage.The above-mentioned diamond-back moth of usining in laboratory freely bred for 3 generations as host.
1.2 host plant
For examination host plant, be wild cabbage, seed is purchased from vegetables institute's special vegetable center, Beijing City Agriculture and Forestry Institute; After feeding block seedlings raising, field planting is in the plastic flowerpot of bore 12cm, and (dependent insect cage is aluminum alloy frame, and specification is 54 * 45 * 40cm then to put into dependent insect cage; Wherein one side is glass sliding door, and bottom surface is glass, and all the other 4 envelopes are with 120 order nylon mesh) inner; Strengthen the water and fertilizer management of host plant, give sufficient illumination, guarantee that its growth conditions is sufficient, cultivates and obtains the clean seedling without worm stalwartness.
1.3 experimental technique
" the whole strain " that adopts this chapter second section to introduce connects worm method and processes cabbage seedling, obtain parasitism have 2 age diamondback moth larvae cabbage seedling; And then adopting diameter 2.5cm, height 12cm " referring to type pipe " connects honeybee method and connects honeybee; By connecing diamondback moth larvae after honeybee, be put on the clean seedling without worm stalwartness and cultivate; On every young plant, put the diamondback moth larvae that 30 left and right physically well develop, crossed by Cotesia plutellae larvae parasitism.Then divide following 3 groups to test, establish 5 repetitions for every group.
First group: by connecing diamondback moth larvae after honeybee, connect and be put on cabbage seedling, put in the lucite dependent insect cage (positive is sliding door, and the back side is 120 order nylon mesh, and there is the nylon mesh of 12 * 18cm left and right side) of 34 * 34 * 28cm, cultivate, observe.
Second group: by connecing diamondback moth larvae after honeybee, connect and be put on Brassica campestris L seedling, put into lucite dependent insect cage (with 1), cultivate, observe.
The 3rd group: by connecing diamondback moth larvae after honeybee, connect and be put on Chinese cabbage seedling, put into lucite dependent insect cage (with 1) and cultivate, observe.
Each group is processed to be all placed in same insectary and is carried out Development culture and observation above, and temperature is 25 ± 1 ℃, the condition of RH.50-60%; L:D=14:10, intensity of illumination is 3000-3500lx left and right.Every mornings 8 point, afternoon 5 records, the developmental state of observing insect, waters to host plant.
1.4 data processings: experiment the data obtained is processed with Excel software, shows comparative result.
2. results and analysis
Experimental result shows: first group of processing finally receives that Cotesia plutellae larvae pupa cocoon number is higher than second group and the 3rd group; Second group higher than the 3rd group.Thereby illustrate, the growing of diamondback moth larvae of different host plants after to parasitism is to have a certain impact, and then the normal growth that has influence on Cotesia plutellae larvae is grown.
Three kinds of host plants of table 2 are raised the situation of the cocooing statistical form of parasitic rear diamond-back moth and Cotesia plutellae larvae
| Host plant | Repeat | Parasitic diamondback moth larvae number | The Cotesia plutellae larvae number of cocooing |
| Cabbage seedling | 10 | 318 | 268 |
| Brassica campestris L seedling | 10 | 326 | 259 |
| Chinese cabbage seedling | 10 | 342 | 236 |
Claims (6)
1. a Cotesia plutellae larvae artificial propagation production method, comprises the following steps:
(1) gather and expand numerous honeybee kind:
field bee kind: occur on serious field plant blade diamond-back moth, gather the pupa of Cotesia plutellae larvae, be i.e. the little cocoon of white;
honeybee kind is purified and is preferred: the little cocoon of the above-mentioned white collecting is placed in to 25-27 ℃, under RH.60-70% condition, grows; Sprout wings and after honeybee, be transferred in the diamond-back moth larva body at 2 initial stages in age latter stage to 3 in age, then pass through parasitic rate, emergence bee hatching rate, fertility, property ratio, storage characteristics and the search capability of target pest, parasitic ability are detected to test, selecting good honeybee kind;
it is numerous that honeybee kind expands: the Cotesia plutellae larvae honeybee kind of selecting is expanded numerous, the Cotesia plutellae larvae honeybee kind of accumulation sufficient amount;
(2) produce host:
(3) inoculation reproduction of diamond-back moth:
after inoculation has been laid eggs, with gobo, cover 4 of dependent insect cage (left and right, upper, below), utilize diamond-back moth adult phototaxis principle, shake the host plant of Oviposition of Diamondback Moth in shrouding, drive diamond-back moth adult on host plant away;
then, 66 basins are wherein taken out of, move into diamondback moth larvae and cultivate growth chamber, cultivate and grow;
(4) diamondback moth larvae is grown:
After inoculation has been laid eggs, with the host plant of Eggs of Diamondback Moth, move into diamondback moth larvae and cultivate growth chamber, and strengthen management, promote leaf growth, standby when diamondback moth larvae is grown to 2 age;
(5) Cotesia plutellae larvae inoculation:
By the host plant with 2-3 diamondback moth larvae in age, be moved into a large amount of Cotesia plutellae larvaes and become the interior inoculation of shrouding of honeybee, there to be Cotesia plutellae larvae 4-6 head to be advisable on every cabbage leaves; After 24-26 hour, utilize Cotesia plutellae larvae to become honeybee phototaxis principle, make seedling and honeybee separately.
(6) Cotesia plutellae larvae is cultivated:
When being grown by 7 days by parasitic diamondback moth larvae, there is Cotesia plutellae larvae mature larva to start to get out in diamondback moth larvae body, within 1-2 hour, can form the cocoon of pupating of white; Answer timely collecting, packing or refrigerate standby.
(7) bee pupa is collected and application:
At Cotesia plutellae larvae mature larva, cocoon the initial stage, should collect in time, store, for dispensing or directly field application.Be difficult for oversize storage period, and along with the prolongation of storage period, the eclosion rate of Cotesia plutellae larvae can reduce gradually along with the prolongation of storage period; Suitably moisturizing of lay up period, to keep the vigor of Cotesia plutellae larvae; Be difficult for oversize storage period, and along with the prolongation of storage period, the eclosion rate of Cotesia plutellae larvae can reduce gradually along with the prolongation of storage period; Generally at 20 days, with interior, be advisable.
(8) reserve seed for planting: when harvesting of cocoon is collected, choose and grow health, individuality is larger, the glossiness Cotesia plutellae larvae cocoon of cocoon shell is as honeybee kind, and the Cotesia plutellae larvae inoculation while being ready for use on next breeding is used.
2. Cotesia plutellae larvae artificial propagation production method as claimed in claim 1, the inoculation reproduction of the described diamond-back moth of step (3) wherein, after inoculation, utilize diamond-back moth adult phototaxis principle, shake the host plant of Oviposition of Diamondback Moth in shrouding, drive diamond-back moth adult away.
3. Cotesia plutellae larvae artificial propagation production method as claimed in claim 1, the wherein described Cotesia plutellae larvae inoculation of step (5), must guarantee in the time of inoculation that the emergence period of Cotesia plutellae larvae and diamondback moth larvae 2-3 match the length of time.
4. Cotesia plutellae larvae artificial propagation production method as claimed in claim 1, the wherein described Cotesia plutellae larvae inoculation of step (5), meets the honeybee worm ratio 1:8-12 of honeybee.
5. Cotesia plutellae larvae artificial propagation production method as claimed in claim 1, the wherein described Cotesia plutellae larvae inoculation of step (5), the Cotesia plutellae larvae male and female of inoculation are than being 6-8:1.
6. Cotesia plutellae larvae artificial propagation production method as claimed in claim 1, the wherein collection of the described Cotesia plutellae larvae cocoon of step (7), the male and female ratio of take when dispensing or field application is advisable as 6-8:1 ratio.
Priority Applications (1)
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| CN108935341A (en) * | 2018-07-27 | 2018-12-07 | 重庆市农业科学院 | A kind of method for breeding of cabbage butterfly braconid wasp |
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| CN104304183A (en) * | 2014-08-27 | 2015-01-28 | 浙江大学 | Artificial propagation and production method for diadromus collaris |
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| CN105165734A (en) * | 2015-09-28 | 2015-12-23 | 中国热带农业科学院环境与植物保护研究所 | Method for mass reproduction of Bracon adoxophyesi Mimanikawa |
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| CN108935341A (en) * | 2018-07-27 | 2018-12-07 | 重庆市农业科学院 | A kind of method for breeding of cabbage butterfly braconid wasp |
| CN109169526A (en) * | 2018-09-11 | 2019-01-11 | 浙江大学 | The artificial propagation production method of the anti-jaw cocoon bee of open arms |
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