CN107787698A - A kind of production method for the seed potato for avoiding soil-borne disease from infecting - Google Patents
A kind of production method for the seed potato for avoiding soil-borne disease from infecting Download PDFInfo
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- CN107787698A CN107787698A CN201711057675.XA CN201711057675A CN107787698A CN 107787698 A CN107787698 A CN 107787698A CN 201711057675 A CN201711057675 A CN 201711057675A CN 107787698 A CN107787698 A CN 107787698A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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Abstract
The present invention relates to a kind of production method for the seed potato for avoiding soil-borne disease from infecting, the production method includes the steps such as the cultivation of Solanaceae class stock, the cultivation of scion potato, grafting, the management of grafting plant, grafting plant transplanting, the shading of scion potato base portion, gas life virus-free potato harvesting.The present invention uses grafting by potato for scion, potato seed is expanded in atmosphere, avoid infecting for soil-borne disease in seed potato production process, individual plant knot potato number can be up to more than 20, improve potato seed breeding coefficient, potato seed periodically can be harvested with the specification of kind by production, seed tuber size is homogeneous.In addition, the present invention be stock using the extremely strong wild Solanaceae plant of resistance, can effectively improve during potato growth to biological and abiotic stress resistance.
Description
【Technical field】
The invention belongs to production technology of crops field.More particularly it relates to one kind avoids soil-borne disease from infecting
Seed potato production method.
【Background technology】
Potato is a kind of asexually propagated crop, is easy to be infected and influenceed by soil-borne disease during production of seed stock
Potato seed quality, particularly in recent years as the matrix of original silkworm egg production is used repeatedly, potato seed field continuous cropping increase, potato seed carries line
The soil-borne diseases such as worm, tar spot, dry rot, shot hole and powdery scab are more and more common.Effective ways there is no to be controlled at present.
On potato grafting cultivation, CN 103004479A are disclosed using potato as stock, and tomato is that scion carries out grafting, can be with
The new method of overground part tomato and underground part potato tubers is harvested simultaneously.CN 101707967 disclose it is a kind of using potato as
Scion, the method with datura grafting culture underglass, reduce the input of fertilizer and pesticide.CN 104737830A disclose Ma Ling
Potato is scion, and wintercherry plant solves the problems, such as potato cross-sterility for the engrafting method of stock.But these prior arts
The single stock that some are selected, engrafting method imperfection, the not high technological deficiency of survival rate also be present, and utilize graft technology
Had not been reported with avoiding soil-borne disease from infecting the method for potato seed.
Therefore, the present inventor on the basis for summarizing prior art, by lot of experiments and analysis, completes finally
The present invention.
【The content of the invention】
[technical problems to be solved]
It is an object of the invention to provide a kind of production method for the seed potato for avoiding soil-borne disease from infecting.
[technical scheme]
The present invention is achieved through the following technical solutions.
The present invention relates to a kind of production method for the seed potato for avoiding soil-borne disease from infecting.
The step of production method, is as follows：
A, Solanaceae class stock is cultivated
Stock seed soaks 30min in the water of 55~60 DEG C of temperature and carries out surface disinfection sterilization, is placed in and is lined with sterile suction
In the culture dish of water paper, in 24 DEG C of temperature, 16 hours dark with carrying out vernalization under conditions of illumination in 8 hours, kinds in incubator
Son is showed money or valuables one carries unintentionally, then allows seed to carry out floating seedlings in floating disc, and seedling, which is grown to 2.8~3.2cm, to be transplanted to black plastic nutritive cube
In, cultivated in plastic canopy, when Seedling Height is 4~6cm, 2 cotyledons, 2~3 true leaves and during the thick 9~10mm of stem, take this
Kind seedling is as stock；
B, scion potato is cultivated
The virus-free potato primary stock that vernalization is handled is seeded in sterilization matrix, is cultivated in plastic canopy and grows to strain
High 7~8cm, 3-4 pieces leaf and the thick 8~9mm of stem are as scion；
C, grafting
Clip step B scion, 2 leaflets topmost are left, remove all other blade, then scion is along stem both sides pair
Claim ground beveling two knives, be cut into wedge shape；Step A stock leaves aerial part 2.8~3.2cm stem sections, and remaining cuts off, from
Rive in the middle part of stem 5mm interfaces；Scion tapered end is inserted into stock interface, both forming layers is matched, it is then thin with plastics
Film environmental sealing prevents moisture evaporation, obtains grafting plant；
D, grafting plant manages
Grafting plant is put into drip irrigation type cold light source phytoclimate case, dim light, relative humidity in light intensity 5000Lux
90%th, 24 DEG C of day temperature, 18 DEG C of nocturnal temperature, daylight are cultivated 6~8 days under conditions of shining each 12h, and grafting plant survives；
E, grafting plant is transplanted
The grafting plant that survives that step D is obtained is transplanted in fly net greenhouse, is applied according to the cultivation flow of routine
Fertilizer, pour water, weeding management；Transplanting crosses seedling-slowing stage in 7 days, removes the plastic sheeting for being wrapped in graft union position；
F, scion potato base portion shading
Grafting plant transplants up to 25~30cm 12~15 days long, then builds shading casing with black plastic cloth so that connects
Fringe potato is in dark state in graft union position and its 15~more than 20cm base portion stipes；
G, gas life virus-free potato harvesting
In grafting plant growth course, outlet raw potato, root are grown on described scion potato base portion stipes
According to production kind of a specification requirement, the raw potato of this gas is periodically harvested as potato seed.
A preferred embodiment of the invention, described stock seed are selected from Solanum Torvum, Tuo Tuosijia, wintercherry
Or datura.
According to another preferred embodiment of the present invention, in step, described stock seed is with weight in concentration
5~7h of seed soaking sterilization in the liquor potassic permanganate of meter 0.01~0.04%；Or described stock seed is in an oven in temperature
Surface disinfection sterilization 24h is carried out under conditions of 55~65 DEG C.
According to another preferred embodiment of the present invention, in stepb, potato primary stock is selected from main breed green grass or young crops potato
No. 9, cooperation 88 or beautiful potato 6.
According to another preferred embodiment of the present invention, in stepb, potato detoxicating processing is taken off through tissue culture stem apex
Poison obtains detoxic seedling and reaches detoxification efficiency, and production obtains original silkworm egg under the conditions of mist training；Potato vernalization processing is in temperature
20 DEG C with it is dark under conditions of carry out.
According to another preferred embodiment of the present invention, in step D, grafting plant is positioned in plastic canopy,
The sunshade net of shading rate 70% is covered, and atomized water spray is carried out into shed, the relative humidity in plastic canopy is maintained at 90%
More than.
According to another preferred embodiment of the present invention, in step E, survive grafting plant and transplant to greenhouse, slow seedling 7
Parcel and the plastic sheeting of graft union position are removed after it, makes graft union position normal growth thickening.
According to another preferred embodiment of the present invention, in step F, black plastic cloth builds the light intensity of shading casing
It is 0Lux.
It is raw on each scion potato base portion stipes in step G according to another preferred embodiment of the present invention
Long gas raw potato harvesting number is 4~6 times.
According to another preferred embodiment of the present invention, in step G, the weight of each gas raw potato is 20~30
Gram.
The present invention is described in more detail below.
The present invention relates to a kind of production method for the seed potato for avoiding soil-borne disease from infecting.
The step of production method, is as follows：
A, Solanaceae class stock is cultivated
Stock seed soaks 30min in the water of 55~60 DEG C of temperature and carries out surface disinfection sterilization, then cuts and breaks in the seed coat, puts
In the culture dish for being lined with sterile blotting paper, in incubator under conditions of 24 DEG C of temperature, 16 hours dark and illumination in 8 hours
Vernalization is carried out, seed is showed money or valuables one carries unintentionally, then allows seed to carry out floating seedlings in floating disc, and seedling, which is grown to 2.8~3.2cm, to be transplanted to black
In plastic nutrition bowl, cultivated in plastic canopy, when Seedling Height be 4~6cm, 2 cotyledons, 2~3 true leaves and stem thick 9~
During 10mm, this seedling is taken as stock；
The present invention seed potato production in, described Solanaceae class stock seed be selected from Solanum Torvum, Tuo Tuosijia,
Wintercherry or datura.These stock seeds are all that the product of existing market sale either picks up from the seed of Wild plant, such as
The Solanum Torvum sold by Shandong Shouguang spring and autumn Zhong Ye Co., Ltd with trade name Solanum Torvum, grand seedling is contained by Shouguang City promoted
Center is with the Tuo Tuosijia of super (improvement Tuo Tuosijia) sale of trade name.Wintercherry and datura seed are to pick up from field grown
Wintercherry and datura plant.In this step, stock seed needs to carry out surface disinfection sterilization, and its main purpose is to prevent
Only pathogen infects stock seedling.
In the present invention, stock the surface of the seed sterilizing method is mainly that the hot-water soak of stock seed, potassium permanganate are molten
Liquid is soaked seed and the roasting kind of baking oven.
Stock seed hot-water soak is to allow stock seed to soak 30min in the water of 55~60 DEG C of temperature.Stock seed exists
Soak time is 30min in water, if the water temperature of immersion stock seed is less than 55 DEG C, without Disinfection Effect；If soak anvil
The water temperature of wooden seed is higher than 60 DEG C, then the plumule of seed can be caused to damage.When stock seed soaks in water, stock in gram
Seed with terms of milliliter the ratio of water be 1：5~15, certainly, this ratio is not particularly critical in the present invention, it is appropriate higher or
It is lower also possible.Should be the water by autoclave sterilization in the water that the step uses.
Liquor potassic permanganate seed soaking is to make stock seed molten for by weight 0.01~0.04% potassium permanganate in concentration
5~7h of seed soaking sterilization in liquid.Seed soaking disinfecting time is 5~7h, if the concentration of liquor potassic permanganate is less than 0.01%, can be
Bactericidal effect unobvious；If the concentration of liquor potassic permanganate is higher than 0.04%, percentage of seedgermination may be impacted；
Similarly, the concentration of liquor potassic permanganate is 0.01~0.04%, if seed soaking disinfecting time is less than 5h, the surface of the seed is still
There are residual pathogenic bacteria；If seed soaking disinfecting time is longer than 7h, make the germination percentage of stock seed reduce；Therefore, stock seed exists
5~7h of seed soaking sterilization is appropriate in 0.01~0.04% liquor potassic permanganate.
Roasting kind of baking oven is to allow the stock seed sterilizing 24h at 55~65 DEG C of temperature in an oven.Disinfection time
For 24h, if oven temperature is less than 55 DEG C, the without Disinfection Effect ... ...；If oven temperature is higher than 65 DEG C, can be right
Seed embryo causes to damage.In the present invention, in incubator under conditions of 24 DEG C of temperature, 16 hours dark and illumination in 8 hours
Carry out vernalization.The incubator that the present invention uses is a kind of illumination box of controllable temperature control light, and it is, for example, grand by Shaoguan Thailand
The incubator that Medical Devices Co., Ltd. is sold with trade name LRH-250_G illumination boxs.The germination accelerating method that the present invention uses
It is a kind of conventional germination accelerating method.Herein, it is dark it should be understood that light intensity be 0Lux, light intensity during illumination is 1500~
2000Lux。
The black plastic nutritive cube that the present invention uses be in this area it is usually used, in current market sales of production
Product, the product e.g. sold by City of Taizhou road and bridge beautiful screen cloth factory with trade name plastic nutrition bowl.
B, scion potato is cultivated
The virus-free potato primary stock that vernalization is handled is seeded in sterilization matrix, is cultivated in plastic canopy and grows to strain
High 7~8cm, 3-4 pieces leaf and the thick 8~9mm of stem are as scion；
Potato detoxicating processing is to obtain detoxic seedling through tissue culture stem apex detoxification to reach detoxification efficiency, and raw under the conditions of mist training
Production obtains original silkworm egg；Potato vernalization processing is carried out under conditions of 20 DEG C of temperature with dark.
The potato primary stock that the present invention uses is selected from main breed green grass or young crops potato 9, cooperation 88 or beautiful potato 6, and they are all
Train what production was cultivated through mist by tuber crops research institute of Yunnan Prov Agriculture University.
The sterilization matrix that the present invention uses is conventional seedbed system matrix.These matrix are to use HTHP conventional sterilization procedures
Sterilized, generally, the bacterial content of these sterilization matrix should be less than 0.001%.
C, grafting
Clip step B scion, 2 leaflets topmost are left, remove all other blade, then scion is along stem both sides pair
Claim ground beveling two knives, be cut into wedge shape；Step A stock leaves aerial part 2.8~3.2cm stem sections, and remaining cuts off, from
Rive in the middle part of stem 5mm interfaces；Scion tapered end is inserted into stock interface, both forming layers is matched, it is then thin with plastics
Film environmental sealing prevents moisture evaporation, obtains grafting plant；
The knife that the step uses is required for using 75% alcohol to carry out disinfection processing.
D, grafting plant manages
Grafting plant is put into drip irrigation type cold light source phytoclimate case, dim light, relative humidity in light intensity 5000Lux
90%th, 24 DEG C of day temperature, 18 DEG C of nocturnal temperature, daylight are cultivated 6~8 days under conditions of shining each 12h, and grafting plant survives；
In this step, grafting plant is positioned in plastic canopy, covers the sunshade net of shading rate 70%, and to arch
Atomized water spray is carried out in canopy, the relative humidity in plastic canopy is maintained at more than 90%.
In the present invention, the basic object that grafting plant is handled under the conditions of so is to improve the survival rate of grafting.
The drip irrigation type cold light source phytoclimate case that the present invention uses is current market sales of product, such as is matched by Ningbo
The product that good fortune laboratory apparatus Co., Ltd is sold with trade name drip irrigation type cold light source phytoclimate box pin.
E, grafting plant is transplanted
The grafting plant that survives that step D is obtained is transplanted in fly net greenhouse, is applied according to the cultivation flow of routine
Fertilizer, pour water, weeding management；Transplanting crosses seedling-slowing stage in 7 days, removes the plastic sheeting for being wrapped in graft union position；
In this step, survive grafting plant to transplant to greenhouse, slow seedling removes parcel and graft union position after 7 days
Plastic sheeting, make graft union position normal growth thickening.
F, scion potato base portion shading
Grafting plant transplants up to 25~30cm 12~15 days long, then builds shading casing with black plastic cloth so that connects
Fringe potato is in dark state in graft union position and its 15~more than 20cm base portion stipes；
In the present invention, it is to make potato base portion stipes grafting plant base portion stipes is in the basic role of dark state
In short-day condition, promote the formation of gas raw potato stem tuber.
In this step, the light intensity that black plastic cloth builds shading casing is 0Lux.
G, gas life virus-free potato harvesting
In grafting plant growth course, outlet raw potato, root are grown on described scion potato base portion stipes
According to production kind of a specification requirement, the raw potato of this gas is periodically harvested as potato seed.
In this step, gas raw potato is to start to expand in stipes base portion to be formed, and is then gradually produced upwards；It is average
Every plant can produce 15~30 gas non-hibernating eggs potatos, and the weight of each gas raw potato is 20~30 grams.It is potato-based in each scion
The gas raw potato harvesting number grown on portion's stipes is 4~6 times.
Using sucrose suspended centrifugal method (Liu Wei will plant pathogeny line insect [M] Beijing：Chinese agriculture publishing house,
2000.) with People's Republic of China's agricultural industry criteria-Germplasm Resources of Farm Crop identification technology code-potato, 2007 enter
Row detection, do not find there is any soil-borne disease to infect using the inventive method production potato seed.
Nematode, tar spot, dry rot, shot hole and powder are carried using the potato seed of existing conventional production of seed stock method production
The soil-borne diseases such as scall.
The present invention avoids the production method for the seed potato that soil-borne disease infects from having following features：
Scion potato can form the raw potato of substantial amounts of gas in growing, and will can be used as potato seed can block in production of seed stock
During soil-borne disease propagation.
Wild Solanaceae class stock plant can reduce the input of fertilizer and pesticide with potato grafting, while also improve potato
To the resistance of biology and abiotic stress.
Production method of the present invention can save production of seed stock cost and input, improve economic benefit.
Production of seed stock is carried out using grafting, is expected to the resistance channel genes in wild stock to potato gas non-hibernating eggs potato
In, it is possible to improve potato seed resistance.
[beneficial effect]
Beneficial effects of the present invention are as follows：
A, scion potato can form the raw potato of a large amount of gas in growing, and can be blocked in production of seed stock process as potato seed
The propagation of middle soil-borne disease.
B, the wild Solanaceae class stock plant such as Solanum Torvum, Tuo Tuosijia, wintercherry, datura is a kind of adaptability, resistance
Extremely strong plant of Solanaceae, the input of fertilizer and pesticide can be reduced using it with potato grafting, while also improves potato to life
The resistance of thing and abiotic stress.
C, the raw potato of gas is to start to expand in stipes base portion to be formed, then gradually caused upwards；Average every plant can produce 15
~30 gas non-hibernating eggs potatos, have saved the production cost and input of potato seed, have largely improved economic benefit.
D, potato is simple to operate with stock grafting as scion, potato seed can be harvested at any time as requested, for Ma Ling
Potato scion cultivated, a potato primary stock can at least cultivate 10 scion seedling graftings on stock, it is possible to reduce potato
Potato seed input during breeding.
E, more and more results of study show, grafting can cause inhereditary material to exchange, and potato seed is produced using graft technology, can
Can be by the resistance channel genes in wild stock into potato gas non-hibernating eggs potato, the later stage, can as in potato seed cultivation
It can be improved in terms of resistance.
【Brief description of the drawings】
Fig. 1 is potato and Solanum Torvum grafting plant early growth character figure；
Fig. 2 is potato and Solanum Torvum grafting plant scion potato stolon generation period figure；
Fig. 3 is that potato expands period photo with the raw potato of Solanum Torvum grafting plant scion potato gas；
Fig. 4 is potato and Solanum Torvum grafting plant scion potato gas non-hibernating eggs potato shaping age photo.
【Embodiment】
The present invention is will be better understood that by following embodiments.
Embodiment 1：The production of seed potato
The implementation steps of the embodiment are as follows：
A, Solanaceae class stock is cultivated
Solanum Torvum stock seed soaks surface disinfection sterilization 30min in the water of 55 DEG C of temperature, then cuts and breaks in the seed coat, puts
In the culture dish for being lined with sterile blotting paper, in 24 DEG C of temperature, dark and 1600Lux illumination in 8 hours in 16 hours in incubator
Under conditions of carry out vernalization, seed is showed money or valuables one carries unintentionally, then allows seed to carry out floating seedlings in floating disc, and seedling, which is grown to 2.8~3.2cm, to be moved
Plant into black plastic nutritive cube, cultivated in plastic canopy, when Seedling Height is 4~6cm, 2 cotyledons, 2~3 true leaves with
During the thick 9~10mm of stem, this seedling is taken as stock；
B, scion potato is cultivated
The virus-free potato primary stock that vernalization is handled is seeded in sterilization matrix, is cultivated in plastic canopy and grows to strain
High 7~8cm, 3~4 leaves and the thick 8~9mm of stem are as scion；
C, grafting
Clip step B scion, 2 leaflets topmost are left, remove all other blade, then scion is along stem both sides pair
Claim ground beveling two knives, be cut into wedge shape；Step A stock leaves aerial part 2.8cm stem sections, and remaining cuts off, from stem
Portion rives 5mm interfaces；Scion tapered end is inserted into stock interface, both forming layers is matched, then with plastic sheeting bag
Wrapping up in sealing prevents moisture evaporation, obtains grafting plant；
Potato is with Solanum Torvum grafting plant early growth character referring to accompanying drawing 1；Stock is with connecing after accompanying drawing 1 shows grafting
The growth of fringe binding site is normal, and the luxuriant stalwartness of plant strain growth.
D, grafting plant manages
Grafting plant is put into drip irrigation type cold light source phytoclimate case, covers the sunshade net of shading rate 70%, and to arch
Atomized water spray is carried out in canopy, the relative humidity in plastic canopy is maintained at more than 90.5%.Dim light, phase in light intensity 5000Lux
To humidity 90%, 24 DEG C of day temperature, 18 DEG C of nocturnal temperature, daylight shine each 12h under conditions of cultivate 6 days, grafting plant into
It is living；
E, grafting plant is transplanted
The grafting plant that survives that step D is obtained is transplanted in fly net greenhouse, is applied according to the cultivation flow of routine
Fertilizer, pour water, weeding management；Transplanting crosses seedling-slowing stage in 7 days, removes the plastic sheeting for being wrapped in graft union position；
F, scion potato base portion shading
Grafting plant transplants up to 25~30cm 12~15 days long, then builds shading casing with black plastic cloth, its light intensity
For 0Lux so that scion potato is in dark state in graft union position and its more than 15cm base portion stipes；
G, gas life virus-free potato harvesting
In grafting plant growth course, outlet raw potato is grown on described scion potato base portion stipes, often
The weight of individual gas raw potato is 20 grams, according to production kind of a specification requirement, periodically harvests the raw potato of this gas as potato seed, harvesting
Number is 4 times.
Potato generates period referring to accompanying drawing 2 with Solanum Torvum grafting plant scion potato stolon；Potato and support
The raw potato of Lu Bamu grafting plant scion potato gas expands period photo referring to accompanying drawing 3；Potato and Solanum Torvum grafting plant
Scion potato gas non-hibernating eggs potato shaping age photo is referring to accompanying drawing 4.
Accompanying drawing 2-4 shows that the raw potato quantity of scion potato gas of grafting plant is more, and growth and development are normal.
Using sucrose suspended centrifugal method (Liu Wei will plant pathogeny line insects, Chinese agriculture publishing house, Beijing, 2000)
With People's Republic of China's agricultural industry criteria《Germplasm Resources of Farm Crop identification technology code-potato, 2007》Examined
Survey, do not find that the embodiment prepares potato seed and has the soil-borne disease such as nematode and stem tuber late blight, shot hole dye to infect.
Embodiment 2：The production of seed potato
The implementation steps of the embodiment are as follows：
A, Solanaceae class stock is cultivated
Tuo Tuosijia stocks seed soaks surface disinfection sterilization 30min in the water of temperature 60 C, then cuts and breaks in the seed coat, puts
In the culture dish for being lined with sterile blotting paper, in incubator under conditions of 24 DEG C of temperature, 16 hours dark and illumination in 8 hours
Vernalization is carried out, seed is showed money or valuables one carries unintentionally, then allows seed to carry out floating seedlings in floating disc, and seedling, which is grown to 2.8~3.2cm, to be transplanted to black
In plastic nutrition bowl, cultivated in plastic canopy, when Seedling Height be 4~6cm, 2 cotyledons, 2~3 true leaves and stem thick 9~
During 10mm, this seedling is taken as stock；
B, scion potato is cultivated
The virus-free potato primary stock that vernalization is handled is seeded in sterilization matrix, is cultivated in plastic canopy and grows to strain
High 7~8cm, 3~4 leaves and the thick 8~9mm of stem are as scion；
C, grafting
Clip step B scion, 2 leaflets topmost are left, remove all other blade, then scion is along stem both sides pair
Claim ground beveling two knives, be cut into wedge shape；Step A stock leaves aerial part 3.2cm stem sections, and remaining cuts off, from stem
Portion rives 5mm interfaces；Scion tapered end is inserted into stock interface, both forming layers is matched, then with plastic sheeting bag
Wrapping up in sealing prevents moisture evaporation, obtains grafting plant；
D, grafting plant manages
Grafting plant is put into drip irrigation type cold light source phytoclimate case, covers the sunshade net of shading rate 70%, and to arch
Atomized water spray is carried out in canopy, the relative humidity in plastic canopy is maintained at more than 90%.It is dim light in light intensity 5000Lux, relative
Humidity 90%, 24 DEG C of day temperature, 18 DEG C of nocturnal temperature, daylight are cultivated 7 days under conditions of shining each 12h, and grafting plant survives；
E, grafting plant is transplanted
The grafting plant that survives that step D is obtained is transplanted in fly net greenhouse, is applied according to the cultivation flow of routine
Fertilizer, pour water, weeding management；Transplanting crosses seedling-slowing stage in 7 days, removes the plastic sheeting for being wrapped in graft union position；
F, scion potato base portion shading
Grafting plant transplants up to 25~30cm 12~15 days long, then builds shading casing with black plastic cloth, its light intensity
For 0Lux so that scion potato is in dark state in graft union position and its more than 20cm base portion stipes；
G, gas life virus-free potato harvesting
In grafting plant growth course, outlet raw potato is grown on described scion potato base portion stipes, often
The weight of individual gas raw potato is 25 grams, according to production kind of a specification requirement, periodically harvests the raw potato of this gas as potato seed, harvesting
Number is 6 times.
Potato and Tuo Tuosijia graftings growing state and the potato of embodiment 1 and Solanum Torvum grafting growing state are non-
It is often close.
The detection method described using embodiment 1 is detected, and does not find that the embodiment prepares potato seed and has nematode and stem tuber
The soil-borne diseases such as late blight, shot hole dye infects.
Embodiment 3：The production of seed potato
The implementation steps of the embodiment are as follows：
A, Solanaceae class stock is cultivated
Wintercherry stock seed seed soaking sterilization 6h in concentration is 0.02% liquor potassic permanganate by weight；Then cut through
Kind skin, is placed in and is lined with the culture dish of sterile blotting paper, in 24 DEG C of temperature, dark and illumination in 8 hours in 16 hours in incubator
Under conditions of carry out vernalization, seed is showed money or valuables one carries unintentionally, then allows seed to carry out floating seedlings in floating disc, and seedling, which is grown to 2.8~3.2cm, to be moved
Plant into black plastic nutritive cube, cultivated in plastic canopy, when Seedling Height is 4~6cm, 2 cotyledons, 2~3 true leaves with
During the thick 9~10mm of stem, this seedling is taken as stock；
B, scion potato is cultivated
The virus-free potato primary stock that vernalization is handled is seeded in sterilization matrix, is cultivated in plastic canopy and grows to strain
High 7~8cm, 3~4 leaves and the thick 8~9mm of stem are as scion；
C, grafting
Clip step B scion, 2 leaflets topmost are left, remove all other blade, then scion is along stem both sides pair
Claim ground beveling two knives, be cut into wedge shape；Step A stock leaves aerial part 3.0cm stem sections, and remaining cuts off, from stem
Portion rives 5mm interfaces；Scion tapered end is inserted into stock interface, both forming layers is matched, then with plastic sheeting bag
Wrapping up in sealing prevents moisture evaporation, obtains grafting plant；
D, grafting plant manages
Grafting plant is put into drip irrigation type cold light source phytoclimate case, covers the sunshade net of shading rate 70%, and to arch
Atomized water spray is carried out in canopy, the relative humidity in plastic canopy is maintained at more than 90%.It is dim light in light intensity 5000Lux, relative
Humidity 90%, 24 DEG C of day temperature, 18 DEG C of nocturnal temperature, daylight are cultivated 8 days under conditions of shining each 12h, and grafting plant survives；
E, grafting plant is transplanted
The grafting plant that survives that step D is obtained is transplanted in fly net greenhouse, is applied according to the cultivation flow of routine
Fertilizer, pour water, weeding management；Transplanting crosses seedling-slowing stage in 7 days, removes the plastic sheeting for being wrapped in graft union position；
F, scion potato base portion shading
Grafting plant transplants up to 25~30cm 12~15 days long, then builds shading casing with black plastic cloth, its light intensity
For 0Lux so that scion potato is in dark state in graft union position and its more than 18cm base portion stipes；
G, gas life virus-free potato harvesting
In grafting plant growth course, outlet raw potato is grown on described scion potato base portion stipes, often
The weight of individual gas raw potato is 28 grams, according to production kind of a specification requirement, periodically harvests the raw potato of this gas as potato seed, harvesting
Number is 5 times.
Potato and the potato of wintercherry grafting growing state and embodiment 1 and Solanum Torvum grafting growing state very phase
Closely.
The detection method described using embodiment 1 is detected, and does not find that the embodiment prepares potato seed and has nematode and stem tuber
The soil-borne diseases such as late blight, shot hole dye infects.
Embodiment 4：The production of seed potato
The implementation steps of the embodiment are as follows：
A, Solanaceae class stock is cultivated
Datura stock seed carries out surface disinfection sterilization 24h under conditions of temperature 60 C in an oven, then cuts through
Kind skin, is placed in and is lined with the culture dish of sterile blotting paper, in 24 DEG C of temperature, dark and illumination in 8 hours in 16 hours in incubator
Under conditions of carry out vernalization, seed is showed money or valuables one carries unintentionally, then allows seed to carry out floating seedlings in floating disc, and seedling, which is grown to 2.8~3.2cm, to be moved
Plant into black plastic nutritive cube, cultivated in plastic canopy, when Seedling Height is 4~6cm, 2 cotyledons, 2~3 true leaves with
During the thick 9~10mm of stem, this seedling is taken as stock；
B, scion potato is cultivated
The virus-free potato primary stock that vernalization is handled is seeded in sterilization matrix, is cultivated in plastic canopy and grows to strain
High 7~8cm, 3~4 leaves and the thick 8~9mm of stem are as scion；
C, grafting
Clip step B scion, 2 leaflets topmost are left, remove all other blade, then scion is along stem both sides pair
Claim ground beveling two knives, be cut into wedge shape；Step A stock leaves aerial part 3.0cm stem sections, and remaining cuts off, from stem
Portion rives 5mm interfaces；Scion tapered end is inserted into stock interface, both forming layers is matched, then with plastic sheeting bag
Wrapping up in sealing prevents moisture evaporation, obtains grafting plant；
D, grafting plant manages
Grafting plant is put into drip irrigation type cold light source phytoclimate case, covers the sunshade net of shading rate 70%, and to arch
Atomized water spray is carried out in canopy, the relative humidity in plastic canopy is maintained at more than 90%.It is dim light in light intensity 5000Lux, relative
Humidity 90%, 24 DEG C of day temperature, 18 DEG C of nocturnal temperature, daylight are cultivated 7 days under conditions of shining each 12h, and grafting plant survives；
E, grafting plant is transplanted
The grafting plant that survives that step D is obtained is transplanted in fly net greenhouse, is applied according to the cultivation flow of routine
Fertilizer, pour water, weeding management；Transplanting crosses seedling-slowing stage in 7 days, removes the plastic sheeting for being wrapped in graft union position；
F, scion potato base portion shading
Grafting plant transplants up to 25~30cm 12~15 days long, then builds shading casing with black plastic cloth, its light intensity
For 0Lux so that scion potato is in dark state in graft union position and its more than 15201816cm base portion stipes；
G, gas life virus-free potato harvesting
In grafting plant growth course, outlet raw potato is grown on described scion potato base portion stipes, often
The weight of individual gas raw potato is 30 grams, according to production kind of a specification requirement, periodically harvests the raw potato of this gas as potato seed, harvesting
Number is 5 times.
The potato and Solanum Torvum grafting growing state of potato and datura grafting growing state and embodiment 1 are very
It is close.
The detection method described using embodiment 1 is detected, and does not find that the embodiment prepares potato seed and has nematode and stem tuber
The soil-borne diseases such as late blight, shot hole dye infects.
Claims (10)
1. a kind of production method for the seed potato for avoiding soil-borne disease from infecting, it is characterised in that the step of the production method such as
Under：
A, Solanaceae class stock is cultivated
Stock seed soaks 30min in the water of 55~60 DEG C of temperature and carries out surface disinfection sterilization, is placed in and is lined with sterile blotting paper
Culture dish in, in incubator 24 DEG C of temperature, 16 hours it is dark with carrying out vernalization, seed dew under conditions of illumination in 8 hours
In vain, then seed is allowed to carry out floating seedlings in floating disc, seedling, which is grown to 2.8~3.2cm, to be transplanted into black plastic nutritive cube,
Cultivated in plastic canopy, when Seedling Height is 4~6cm, 2 cotyledons, 2~3 true leaves and during the thick 9~10mm of stem, take this children
Seedling is as stock；
B, scion potato is cultivated
The virus-free potato primary stock that vernalization is handled is seeded in sterilization matrix, is cultivated in plastic canopy and grows to plant height 7
~8cm, 3-4 pieces leaf and the thick 8~9mm of stem are as scion；
C, grafting
Clip step B scion, leave topmost 2 leaflets, remove all other blade, then scion along stem both sides symmetrically
Two knives are chamfer, are cut into wedge shape；Step A stock leaves aerial part 2.8~3.2cm stem sections, and remaining cuts off, from stem
Portion rives 5mm interfaces；Scion tapered end is inserted into stock interface, both forming layers is matched, then with plastic sheeting bag
Wrapping up in sealing prevents moisture evaporation, obtains grafting plant；
D, grafting plant manages
Grafting plant is put into drip irrigation type cold light source phytoclimate case, dim light, relative humidity 90% in light intensity 5000Lux,
24 DEG C of day temperature, 18 DEG C of nocturnal temperature, daylight are cultivated 6~8 days under conditions of shining each 12h, and grafting plant survives；
E, grafting plant is transplanted
The grafting plant that survives that step D is obtained is transplanted in fly net greenhouse, is applied fertilizer, filled according to the cultivation flow of routine
Water, weeding management；Transplanting crosses seedling-slowing stage in 7 days, removes the plastic sheeting for being wrapped in graft union position；
F, scion potato base portion shading
Grafting plant transplants up to 25~30cm 12~15 days long, then builds shading casing with black plastic cloth so that scion horse
Bell potato is in dark state in graft union position and its 15~more than 20cm base portion stipes；
G, gas life virus-free potato harvesting
In grafting plant growth course, outlet raw potato is grown on described scion potato base portion stipes, according to life
Production kind of a specification requirement, periodically harvests the raw potato of this gas as potato seed.
2. method for preparing seed potato according to claim 1, it is characterised in that described stock seed is selected from support Shandong
Ba Mu, Tuo Tuosijia, wintercherry or datura.
3. method for preparing seed potato according to claim 1 or 2, it is characterised in that in step, described stock
Seed 5~7h of seed soaking sterilization in concentration is 0.01~0.04% liquor potassic permanganate by weight；Or described stock
Seed carries out surface disinfection sterilization 24h under conditions of 55~65 DEG C of temperature in an oven.
4. method for preparing seed potato according to claim 1, it is characterised in that in stepb, potato primary stock
Selected from main breed green grass or young crops potato 9, cooperation 88 or beautiful potato 6.
5. method for preparing seed potato according to claim 1, it is characterised in that in stepb, at potato detoxicating
Reason is to obtain detoxic seedling through tissue culture stem apex detoxification to reach detoxification efficiency, and production obtains original silkworm egg under the conditions of mist training；Potato
Vernalization processing is carried out under conditions of 20 DEG C of temperature with dark.
6. method for preparing seed potato according to claim 1, it is characterised in that in step E, grafting plant is put
It is placed in plastic canopy, covers the sunshade net of shading rate 70%, and atomized water spray is carried out into shed, makes relative in plastic canopy
Humidity is maintained at more than 90%.
7. method for preparing seed potato according to claim 1, it is characterised in that in step E, survive grafting plant
To greenhouse, slow seedling removes parcel and the plastic sheeting of graft union position after 7 days for transplanting, increases graft union position normal growth
Slightly.
8. method for preparing seed potato according to claim 1, it is characterised in that in step F, black plastic cloth is taken
The light intensity for building shading casing is 0Lux.
9. method for preparing seed potato according to claim 2, it is characterised in that in step G, in each scion horse
The gas raw potato harvesting number grown on bell potato base portion stipes is 4~6 times.
10. method for preparing seed potato according to claim 2, it is characterised in that in step G, each gas gives birth to Ma Ling
The weight of potato is 20~30 grams.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108934872A (en) * | 2018-07-23 | 2018-12-07 | 贵州申黔农业科技有限公司 | A kind of high starch, high yield sweet potato breeding method |
| CN109156188A (en) * | 2018-09-07 | 2019-01-08 | 云南农业大学 | Utilize the method for the graft technology prevention and treatment late blight of potato |
| CN113207456A (en) * | 2021-03-12 | 2021-08-06 | 广西壮族自治区农业科学院 | Method and device for creating cold-resistant germplasm of potatoes |
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| CN1356022A (en) * | 2001-12-18 | 2002-07-03 | 何流 | Method for grafting mentor seeding to solanaceous crops |
| CN101707967A (en) * | 2009-12-01 | 2010-05-19 | 詹志翔 | Potato grafting cultivation method |
| CN103477876A (en) * | 2013-09-23 | 2014-01-01 | 青海省农林科学院 | Potato crossbreeding method |
| CN104737830B (en) * | 2015-04-14 | 2017-05-03 | 河北北方学院 | Method for solving infertility of potato varieties serving as hybrid female parents |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1356022A (en) * | 2001-12-18 | 2002-07-03 | 何流 | Method for grafting mentor seeding to solanaceous crops |
| CN101707967A (en) * | 2009-12-01 | 2010-05-19 | 詹志翔 | Potato grafting cultivation method |
| CN103477876A (en) * | 2013-09-23 | 2014-01-01 | 青海省农林科学院 | Potato crossbreeding method |
| CN104737830B (en) * | 2015-04-14 | 2017-05-03 | 河北北方学院 | Method for solving infertility of potato varieties serving as hybrid female parents |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108934872A (en) * | 2018-07-23 | 2018-12-07 | 贵州申黔农业科技有限公司 | A kind of high starch, high yield sweet potato breeding method |
| CN109156188A (en) * | 2018-09-07 | 2019-01-08 | 云南农业大学 | Utilize the method for the graft technology prevention and treatment late blight of potato |
| CN113207456A (en) * | 2021-03-12 | 2021-08-06 | 广西壮族自治区农业科学院 | Method and device for creating cold-resistant germplasm of potatoes |
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