CN106106192A - A kind of method for building up of Garbo fruit tissue culturing system - Google Patents
A kind of method for building up of Garbo fruit tissue culturing system Download PDFInfo
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- CN106106192A CN106106192A CN201610746488.1A CN201610746488A CN106106192A CN 106106192 A CN106106192 A CN 106106192A CN 201610746488 A CN201610746488 A CN 201610746488A CN 106106192 A CN106106192 A CN 106106192A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
The invention discloses the method that a kind of Garbo fruit tissue culturing system sets up, by the method quickly obtaining a large amount of Garbos fruit improved seeds test tube Seedling in the plant tissue culture technique short time.The present invention is outer implant with Garbo stem end point, it is effectively improved the inductivity of bud, subculture coefficient and rooting rate through processes such as outer implant sterilization, Primary culture, enrichment culture, strong seedling culture, root culture, seedling exercising and transplantings, and then establish complete Garbo fruit tissue culturing system, in order to carry out Garbo fruit seedling factorial praluction.The fruit that the application present invention obtains can alcoholic, make fruit jelly, fruit jam, human body had health-care effect, prevent cardiovascular diseases, build up one's resistance to disease, skin protection skin lightening.
Description
Technical field
The present invention relates to woods fruit tree field, the method for building up of a kind of Garbo fruit tissue culturing system.
Background technology
The characteristic trait of Garbo fruit is green throughout the year, poor growth, tree-like grace, again will not parasitic scale insect, aphid.Fruit
Both edible, is also available for people and views and admires, apply plant at balcony basin, garden landscape or potted plant enjoying all are extremely suitable.Fruit can be eaten raw,
Also Eaux-De-Vie, fruit juice, ice cream and cake stuffing material etc. can be processed as.The only easy dehydration of fresh fruit, deposits under general room temperature 2-3 days
I.e. can ferment, be weak to storage, greatly limit the expansion in its fresh fruit market, thus while Garbo fruit peel is hard, but gather
During it should be noted that collision free and cause wound, in order to avoid fruit damage, dehydration or bacteria infection and accelerate to rot.Foreign study
For fruit table coating fruit is cured or bag plastic foil is placed under low temperature environment, can extend the shelf-life of Garbo fruits, this is preliminary
Result of the test be, after the fruit after adopting removes field heat, to put into polyethylene plastic bag as quickly as possible, mistake can be reduced under low temperature
Water and Fermentation, extending shelf-life is 2 weeks, and display improves packaging material can effectively extend Garbo with suitable reserve temperature
The fresh fruit life-span of fruit, if can include in production system by this postharvest handling method, it is believed that Garbo fruit should be able to more be popularized in Taiwan
In fruit market.Every hectogram edible fruit part heat content 45.7Ka of Garbo fruit.Water 87.1g, protein 0.1lg, fat
0.01g, carbohydrate 12.58g, fiber 0.08g, ash 0.20g, calcium 6.3mg, phosphorus 9.2mg, ferrum 0.49rag, thiamine
0.2mg, vitamin B z 0.02rag, nicotinic acid 0.21rrg, Catergen 2.7mg, tryptophan lmg, lysine 7mg.High nutrition becomes
Part, precious, rare, its sarcocarp is transparent, soft and succulency, local flavor are special, fragrant, sarcocarp rich in moisture, vitamin C, high calcium,
15 kinds of phosphorus, ferrum, fiber, carbohydrate, Vitamin B1, calorie, riboflavin, tryptophane, ash, fat, protein etc.
The highest nutrition.Fresh fruit is in addition to can eating something rare, it is also possible to make fruit jelly, preserve, fruit jam and local flavor pole
Good Garbo fruit wine is often edible to be conducive to preventing cardiovascular disease, builds up resistance, especially vigor youth, the skin to women
Skin is beautiful remarkably promotes effect.Its sarcocarp of mellow fruit is that succulence is translucent, and sweet incomparable time well done, taste is special.Ripe
Really its sarcocarp is transparent, soft and succulency, and local flavor is special, fragrant, and sugariness is averagely up to 13 to 18 degree, and fragrant and sweet good to eat, mouthfeel is only
Spy, taste is like multiple local flavors such as Garcinia mangostana, fragrant Fructus psidii guajavae immaturus, Sakyamuni, Fructus Ananadis comosis, and after edible entrance, mouthfeel will experience the unique taste of 4 kinds of differences.
Sunlight: Garbo fruit is evergreen fruit trees, and property happiness is warm, high temperature resistant.Temperature: the autumn to spring is peak of growing season, optimum temperature 22 DEG C ~ 35
℃.Soil: Garbo fruit is suitable for cultivation and grows up best in Acid soil.Fertilising: available water soluble fertilizers or long-lasting fertilizer.
Prune: potted plant often repair point depending on appearance demand；General then need not.Moisture content: plant ground plant and preferably increase soil around earth's surface and carry, in order to
Pour water irrigation.Propagation method, sows or inserts.
Summary of the invention
It is an object of the invention to provide a kind of Garbo fruit method for tissue culture, the present invention is with Garbo stem end point as outer planting
Body, through processes such as outer implant sterilization, Primary culture, enrichment culture, strong seedling culture, root culture, seedling exercising and transplantings effectively
Improve the inductivity of bud, subculture coefficient and rooting rate, and then establish complete Garbo fruit tissue culturing system, thus realize
The purpose of the present invention.Having process simple, workable, inductivity improves, many fruit features of taking root.
The technical solution of the present invention is such, the method that a kind of Garbo fruit tissue culturing system sets up, its feature
It is to comprise the following steps:
Step 1, outer implant is sterilized: takes the stem apex that healthy and strong Garbo fruit is raw then, after scrubbing clean, soaks with washing powder solution
Scrub outer planting surface with hairbrush after 12min dust and the part thalline on its surface to be removed, after then rinsing 5h with tap water
In superclean bench, first with after 75% ethanol disinfection 32s with aseptic washing 8 times, then with 0.1% mercuric chloride solution sterilization 28min, by nothing
Bacterium water is standby after rinsing the globule drying surface for 8 times again with aseptic filter paper;
Step 2, Primary culture: the stem apex after step (1) processes is inoculated into Primary culture base and carries out bud inducement, after inoculation first
Under the conditions of 30 DEG C, full light culture adds up pollution rate and survival rate, then illumination every day 15 hours after 9 days, and intensity of illumination is
Cultivate 32 days under the conditions of 3200lx, the rate of sprouting of statistics tissue cultured seedling and growing state;
Step 3, enrichment culture: growth step (2) cultivation obtained is normal, have obvious stem, the Multiple Buds of impeller structure cuts also
Proceeding to carry out on proliferated culture medium successive transfer culture, first full light culture 5 days under the conditions of 29 DEG C after inoculation, then illumination every day 15 is little
Time, intensity of illumination is 3000lx, and cultivation temperature observes growing state and Shoot propagation situation after cultivating 32 days under conditions of being 29 DEG C,
Cutting sprout carries out successive transfer culture, to obtain more Multiple Buds the most repeatedly;
Step 4, strong seedling culture: by being cut into appropriately sized through the tufted seedling of step (3) enrichment culture, be inoculated on strong seedling culture base
Carry out strong seedling culture, be beneficial to take root, first full light culture 5 days, then illumination every day 15 hours, light under the conditions of 29 DEG C after inoculation
Being 3200lx according to intensity, cultivation temperature is cultivated under conditions of being 29 DEG C to tissue cultured seedling and is grown into more than 4cm;
Step 5, root culture: the tissue culture plant inoculation of more than height 4.0cm step (2), (3) or (4) process obtained is to raw
Root culture is carried out, first full light culture 5 days, then illumination every day 16 hours, light under the conditions of 29 DEG C after inoculation in root culture medium
Being 3200lx according to intensity, cultivation temperature adds up, after cultivating 1 month under conditions of being 29 DEG C, situation of taking root;
Step 6, acclimatization and transplants: the high well-grown of about 9cm step (5) obtained and the rooting tube plantlet of stalwartness go bottle cap certainly
So illumination lower refining seedling is after 9 days, is taken out by test tube Seedling, washes root culture medium off, plant into by Nutrition Soil from culture bottle: perlite=
The substrate that 4:1 is mixed into, cultivates in illumination box, waters to seedling with 1/4MS macro-element nutrients liquid every day, keeps humidity
, after seedling survives, transplant land for growing field crops again.
Primary culture base described in step (2) is: MS+2mg/L6-BA+0.7mg/L NAA+32g/L sucrose+7.0g/L
Agar, pH is 5.9.
Proliferated culture medium described in step (3) is: MS+1.2mg/LNAA+7mg/L 6-BA+32g/L sucrose+7.5g/L fine jade
Fat, pH is 5.9.
Strong seedling culture base described in step (4) is: MS+0.8mg/L 6-BA+1.2mg/L GA3+ 32g/L sucrose+
7.5g/L agar, pH is 5.9.
Root media described in step (5) is: 1/2MS+6.5mg/L IBA+32g/L sucrose+7.0g/L agar, pH
It is 5.9.
The invention have the advantage that by quickly obtaining a large amount of Garbos fruit improved seeds in the plant tissue culture technique short time
The method of test tube Seedling.The present invention is outer implant with Garbo stem end point, through outer implant sterilization, Primary culture, enrichment culture, strong sprout
The processes such as cultivation, root culture, seedling exercising and transplanting are effectively improved the inductivity of bud, subculture coefficient and rooting rate, Jin Erjian
Found complete Garbo fruit tissue culturing system, in order to carry out Garbo fruit seedling factorial praluction.Fruit can make fruit jelly, really
Beans, fruit wine, have health-care effect, prevents cardiovascular diseases, builds up one's resistance to disease, skin protection skin lightening.
Detailed description of the invention
Example below is to further illustrate the present invention.
Embodiment 1
(1) seminal propagation: this is the Garbo current topmost propagation method of fruit, and every fruit has 1 to 4, seed, in irregular rib
Type, for polyembryony.Fresh seeds is germinateed preferably, extrudes seed after typically being crushed by peel, and after 1-2 day, sarcocarp acidifying is rotted,
Again with clear water eccysis sarcocarp, can sow.Plastic bag it is stored in after fruit harvesting, cold preservation at 5-8 DEG C, further take out time to be sowed
Seed is sowed after cleaning, and cold preservation can maintain 1-2 month, still has certain germination percentage.Seed germinates after sowing 20-40 days, gas
Germinate when temperature is low slower.
(2) grafting: the nursery stock of growing directly from seeds of most Garbos fruit kind needs 6-10 can start result, utilizes grafting to do sth. in advance
As a result, after grafting about 3 years can result, select biennial nursery stock as stock, result elite stand clip annotinous branch be scion,
In cut-grafting autumn and winter or to be abutted against survival rate higher, rate of taking over a job after the 3-4 month is poor, and summer grafting is difficult to successfully.
(3) cuttage: Garbo fruit is difficult to cuttage survival, studies in China its be the difficult root of hair species of typical case, can be in the annual May
With semihard stem cutting (2 4 leaves of joint), speed leaching NAA 2000 ppm, with peat soil as rooting media, Rooting percent is up to ninety percent.And state
Outer research is then that band 3-4 is to climax leaves, and in the medium of sand mixing peat soil (ratio 1:1), cuttage is with 10 centimeters of long cutting
Good, cutting base portion rip cutting 4 adds IBA 1000 ppm process can promote root of hair.(4) prune: because of Garbo fruit fertility slowly, at ordinary times
Need not cut by force, but for controlling plant height and potted plant tree-shaped, prune the upper suggestion of the branch that apical growth is vigorous, generally cultivation the most in good time and repair
Cut lower and interior detail brachyplast bar, to mould ventilation and the tree-shaped of branch grace, close life or excessive growth branch can be pruned another autumn and winter.
(4) seedling management: is that the tip is determined in bud picking, erases double bud, many head-germs, crosses weak bud after rudiment.After forming young sprout, toward both sides
Iron wire draws to be tied up, and determines the tip by 15～20cm spacing and sprays rush flower king No. 3 in time, can the effective mad length of control shoot, promote to take out spray more, rush is spent
Bud polarization.Can efficient balance size spica, save flower and fruit thinning tired.Will topdress according to growth stage, i.e. flowers and fruits are fertile, really in good time
Swollen fertilizer, coloring are fertile and expedite the emergence of fertilizer after adopting.It is important to note that accomplish the Proper Match of each seed manure such as NPK element, it is ensured that nutrition equal
Weighing apparatus supply.
(5) pest management: Garbo fruit is shallow root, shrubbery fruit tree, and these seeds are strong to pest and disease damage drag, and pest and disease damage is few, management
On use pesticide less, be the seeds of the best treatment of planting.The pest and disease damage that Garbo fruit cultivates generation at home is few, in management very
Use pesticide less, be the seeds of the best treatment of planting.Only easily by bird pest during result, black net can be covered in plant periphery
Or set up solarium and prevent birdseed, also can be with double-deck newspaper bagging to protect fruit.If there being acute tonsillitis class larva to endanger blade, can use
Plant protection handbook recommended drug is prevented and treated.Abroad, damp and hot season is susceptible to rust, can improve plant interior lights by pruning
Line and improve ventilation and prevent, can spray antibacterial to reduce cause of disease density during generation.Additionally aphid, fruit fly, scale insect and
Beetle abroad has the record of generation, should note these pest and disease damages during domestic cultivation in the lump.
Embodiment 2
(1) outer implant sterilization: take the stem apex that healthy and strong Garbo fruit is raw then, after scrubbing clean, after soaking 6min with washing powder solution
Scrub outer planting surface gently dust and the part thalline on its surface to be removed with banister brush, then rinse 1h with tap water rearmounted
In superclean bench, first with after 75% ethanol disinfection 6s with aseptic washing 4 times, then with 0.1% mercuric chloride solution sterilization 10min, use
Aseptic water washing is standby dry the globule on surface for 5 times again with aseptic filter paper after.
(2) Primary culture: the stem apex after step (1) processes is inoculated into Primary culture base and carries out bud inducement, after inoculation
First 5 days after stain rates as little as 3% of full light culture under the conditions of 26 DEG C, survival rate reaches 78%.Then illumination every day 12 hours, illumination is strong
Degree is for cultivating 32 days under the conditions of 1600x, and the rate of sprouting of tissue cultured seedling is 70%.Described Primary culture base is: MS+0.6mg/L6-BA
+ 0.2mg/L NAA+30g/L sucrose+4.5g/L agar, pH is 5.9.
(3) enrichment culture: growth step (2) cultivation obtained is normal, have obvious stem, the Multiple Buds of impeller structure cuts also
Proceeding to carry out on proliferated culture medium successive transfer culture, first full light culture 1 day under the conditions of 28 DEG C after inoculation, then illumination every day 10 is little
Time, intensity of illumination is 1000lx, and after cultivation temperature is cultivated 30 days under conditions of being 28 DEG C, Shoot propagation coefficient is 4.89.The most repeatedly
Cutting sprout carries out successive transfer culture, to obtain more Multiple Buds.Described proliferated culture medium is: MS+0.5mg/LNAA+3mg/
L 6-BA+15g/L sucrose+4.0g/L agar, pH is 5.8.
(4) strong seedling culture: by being cut into appropriately sized through the tufted seedling of step (3) enrichment culture, be inoculated into strong seedling culture base
On carry out strong seedling culture, be beneficial to take root.After inoculation, first full light culture 1 day under the conditions of 28 DEG C, is subsequently placed in illumination every day 10
Hour, intensity of illumination is 2000lx, and cultivation temperature cultivates 15 days tissue cultured seedlinies under conditions of being 28 DEG C can grow into more than 2cm.
Described strong seedling culture base is: MS+0.3mg/L 6-BA+0.05/L GA3+ 15g/L sucrose+3.5g/L agar, pH is 5.8.
(5) root culture: the tissue culture plant inoculation of more than height 2.0cm step (2), (3) or (4) process obtained is to raw
Carrying out root culture in root culture medium, after inoculation, first full light culture 1 day under the conditions of 28 DEG C, is subsequently placed in illumination every day 10 little
Time, intensity of illumination is 2000lx, and after cultivation temperature is cultivated 1 month under conditions of being 26 DEG C, rooting rate reaches 93.5%.Described takes root
Culture medium is: 1/2MS+2mg/L IBA+15g/L sucrose+3.5g/L agar, pH is 5.8.
(6) acclimatization and transplants: the high well-grown of about 5.5cm and the rooting tube plantlet of stalwartness that step (5) are obtained remove bottle
After lid is placed in natural lighting lower refining seedling 4 days, test tube Seedling is taken out from culture bottle, washes root culture medium off, plant into by Nutrition Soil:
The substrate that perlite=3:1 is mixed into, cultivates in illumination box, waters to seedling with 1/4MS macro-element nutrients liquid every day,
Keep humidity.Transplanting land for growing field crops after seedling survives again, transplanting survival rate is more than 96%.
Embodiment 3:
(1) outer implant sterilization: take the stem apex that healthy and strong Garbo fruit is raw then, after scrubbing clean, after soaking 8min with washing powder solution
Scrub outer planting surface gently dust and the part thalline on its surface to be removed with banister brush, super after then rinsing 3h with tap water
In clean workbench, first with after 75% ethanol disinfection 12s with aseptic washing 5 times, then sterilize 14min with 0.1% mercuric chloride solution, with aseptic
Water is standby after rinsing the globule drying surface for 6 times again with aseptic filter paper.
(2) Primary culture: the stem apex after step (1) processes is inoculated into Primary culture base and carries out bud inducement, after inoculation
First 4 days after stain rates as little as 5% of full light culture under the conditions of 28 DEG C, survival rate reaches 81.6%.Then illumination every day 13 hours, illumination
Intensity is to cultivate 29 days under the conditions of 1400x, and the rate of sprouting of tissue cultured seedling is 79%.Described Primary culture base is: MS+0.8mg/L6-
BA+0.1mg/L NAA+30g/L sucrose+4.5g/L agar, pH is 5.7.
(3) enrichment culture: growth step (2) cultivation obtained is normal, have obvious stem, the Multiple Buds of impeller structure cuts also
Proceeding to carry out on proliferated culture medium successive transfer culture, first full light culture 3 days under the conditions of 28 DEG C after inoculation, then illumination every day 10 is little
Time, intensity of illumination is 1500lx, and cultivation temperature cultivates Shoot propagation coefficient 5.13 after 30 days under conditions of being 28 DEG C.The most repeatedly cut
Cut sprout and carry out successive transfer culture, to obtain more Multiple Buds.Described proliferated culture medium is: MS+0.3mg/LNAA+5mg/L
6-BA+25g/L sucrose+4.5g/L agar, pH is 5.8.
(4) strong seedling culture: by being cut into appropriately sized through the tufted seedling of step (3) enrichment culture, be inoculated into strong seedling culture base
On carry out strong seedling culture, be beneficial to take root.First full light culture 2 days under the conditions of 28 DEG C after inoculation, then illumination every day 11 hours,
Intensity of illumination is 2500lx, and cultivation temperature cultivates 17 days tissue cultured seedlinies under conditions of being 28 DEG C can grow into more than 3cm.Described
Strong seedling culture base is: MS+0.4mg/L 6-BA+0.1/L GA3+ 15g/L sucrose+3.5g/L agar, pH is 5.8.
(5) root culture: the tissue culture plant inoculation of more than height 2cm step (2), (3) or (4) process obtained is to taking root
Root culture is carried out, first full light culture 1 day, then illumination every day 10 hours, illumination under the conditions of 26 DEG C after inoculation in culture medium
Intensity is 2500lx, and after cultivation temperature is cultivated 29 days under conditions of being 28 DEG C, rooting rate reaches 96%.Described root media is:
1/2MS+4mg/L IBA+18g/L sucrose+3.7g/L agar, pH is 5.8.
(6) acclimatization and transplants: the high well-grown of about 2cm and the rooting tube plantlet of stalwartness that step (5) are obtained remove bottle cap
After natural lighting lower refining seedling 5 days, test tube Seedling is taken out from culture bottle, wash root culture medium off, plant into by Nutrition Soil: perlite
The substrate that=3:1 is mixed into, cultivates in illumination box, waters to seedling with 1/4MS macro-element nutrients liquid, keep wet every day
Degree.Transplanting land for growing field crops after seedling survives again, transplanting survival rate is more than 93%.
Claims (5)
1. the method that a Garbo fruit tissue culturing system sets up, it is characterised in that comprise the following steps:
Step 1, outer implant is sterilized: takes the stem apex that healthy and strong Garbo fruit is raw then, after scrubbing clean, soaks with washing powder solution
Scrub outer planting surface with hairbrush after 12min dust and the part thalline on its surface to be removed, after then rinsing 5h with tap water
In superclean bench, first with after 75% ethanol disinfection 32s with aseptic washing 8 times, then with 0.1% mercuric chloride solution sterilization 28min, by nothing
Bacterium water is standby after rinsing the globule drying surface for 8 times again with aseptic filter paper;
Step 2, Primary culture: the stem apex after step (1) processes is inoculated into Primary culture base and carries out bud inducement, after inoculation first
Under the conditions of 30 DEG C, full light culture adds up pollution rate and survival rate, then illumination every day 15 hours after 9 days, and intensity of illumination is
Cultivate 32 days under the conditions of 3200lx, the rate of sprouting of statistics tissue cultured seedling and growing state;
Step 3, enrichment culture: growth step (2) cultivation obtained is normal, have obvious stem, the Multiple Buds of impeller structure cuts also
Proceeding to carry out on proliferated culture medium successive transfer culture, first full light culture 5 days under the conditions of 29 DEG C after inoculation, then illumination every day 15 is little
Time, intensity of illumination is 3000lx, and cultivation temperature observes growing state and Shoot propagation situation after cultivating 32 days under conditions of being 29 DEG C,
Cutting sprout carries out successive transfer culture, to obtain more Multiple Buds the most repeatedly;
Step 4, strong seedling culture: by being cut into appropriately sized through the tufted seedling of step (3) enrichment culture, be inoculated on strong seedling culture base
Carry out strong seedling culture, be beneficial to take root, first full light culture 5 days, then illumination every day 15 hours, light under the conditions of 29 DEG C after inoculation
Being 3200lx according to intensity, cultivation temperature is cultivated under conditions of being 29 DEG C to tissue cultured seedling and is grown into more than 4cm;
Step 5, root culture: the tissue culture plant inoculation of more than height 4.0cm step (2), (3) or (4) process obtained is to raw
Root culture is carried out, first full light culture 5 days, then illumination every day 16 hours, light under the conditions of 29 DEG C after inoculation in root culture medium
Being 3200lx according to intensity, cultivation temperature adds up, after cultivating 1 month under conditions of being 29 DEG C, situation of taking root;
Step 6, acclimatization and transplants: the high well-grown of about 9cm step (5) obtained and the rooting tube plantlet of stalwartness go bottle cap certainly
So illumination lower refining seedling is after 9 days, is taken out by test tube Seedling, washes root culture medium off, plant into by Nutrition Soil from culture bottle: perlite=
The substrate that 4:1 is mixed into, cultivates in illumination box, waters to seedling with 1/4MS macro-element nutrients liquid every day, keeps humidity
, after seedling survives, transplant land for growing field crops again.
The method that a kind of Garbo the most according to claim 1 fruit tissue culturing system sets up, it is characterised in that step (2)
Described Primary culture base is: MS+2mg/L6-BA+0.7mg/L NAA+32g/L sucrose+7.0g/L agar, pH is 5.9.
The method that a kind of Garbo the most according to claim 1 fruit tissue culturing system sets up, it is characterised in that step (3)
Described proliferated culture medium is: MS+1.2mg/LNAA+7mg/L 6-BA+32g/L sucrose+7.5g/L agar, pH is 5.9.
The method that a kind of Garbo the most according to claim 1 fruit tissue culturing system sets up, it is characterised in that step (4)
Described strong seedling culture base is: MS+0.8mg/L 6-BA+1.2mg/L GA3+ 32g/L sucrose+7.5g/L agar, pH is 5.9.
The method that a kind of Garbo the most according to claim 1 fruit tissue culturing system sets up, it is characterised in that step (5) institute
The root media stated is: 1/2MS+6.5mg/L IBA+32g/L sucrose+7.0g/L agar, pH is 5.9.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107347636A (en) * | 2017-06-23 | 2017-11-17 | 中国热带农业科学院热带生物技术研究所 | A kind of detoxification micro-propagation method of Garbo fruit |
| CN108739381A (en) * | 2018-05-28 | 2018-11-06 | 广西亿绿千城农业科技有限公司 | A kind of method for tissue culture of Garbo fruit |
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