CN105706897B - Corn and the distant hybridization method of paddy rice - Google Patents
Corn and the distant hybridization method of paddy rice Download PDFInfo
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- CN105706897B CN105706897B CN201410726900.4A CN201410726900A CN105706897B CN 105706897 B CN105706897 B CN 105706897B CN 201410726900 A CN201410726900 A CN 201410726900A CN 105706897 B CN105706897 B CN 105706897B
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- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 57
- 235000009566 rice Nutrition 0.000 title claims abstract description 55
- 240000008042 Zea mays Species 0.000 title claims abstract description 44
- 235000002017 Zea mays subsp mays Nutrition 0.000 title claims abstract description 44
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 title claims abstract description 36
- 235000005822 corn Nutrition 0.000 title claims abstract description 36
- 238000000034 method Methods 0.000 title claims abstract description 16
- 238000009396 hybridization Methods 0.000 title claims abstract description 13
- 240000007594 Oryza sativa Species 0.000 title description 3
- 241000209094 Oryza Species 0.000 claims abstract description 57
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims abstract description 8
- 235000009973 maize Nutrition 0.000 claims abstract description 8
- 206010020649 Hyperkeratosis Diseases 0.000 claims abstract description 6
- 230000000366 juvenile effect Effects 0.000 claims abstract description 6
- 239000002689 soil Substances 0.000 claims abstract description 5
- 230000007613 environmental effect Effects 0.000 claims abstract description 4
- 239000011159 matrix material Substances 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 230000008961 swelling Effects 0.000 claims description 4
- 238000012549 training Methods 0.000 claims description 4
- 210000001161 mammalian embryo Anatomy 0.000 claims 1
- 238000007654 immersion Methods 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 20
- 238000005516 engineering process Methods 0.000 description 6
- 238000001467 acupuncture Methods 0.000 description 5
- 230000012010 growth Effects 0.000 description 4
- 230000001568 sexual effect Effects 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000012214 genetic breeding Methods 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 208000003643 Callosities Diseases 0.000 description 1
- 241000209510 Liliopsida Species 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 241000209149 Zea Species 0.000 description 1
- 230000004520 agglutination Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000009402 cross-breeding Methods 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000010030 laminating Methods 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 230000005868 ontogenesis Effects 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention relates to a kind of corn and the distant hybridization method of paddy rice, it is divided into Juvenile stage pretreatment, grafting, nursery and four steps of field planting, the first step, Juvenile stage adapts to the kind of local environmental conditions, and pretreatment includes seed immersion and vernalization；Second step, grafting：By the oblique excision part of maize germ, oblique to cut paddy rice plumule and fit to paddy rice plumule on maize germ otch rapidly, scion is fitted with stock, and center is interspersed to fix label, tightens junction surface；3rd step, nursery：The grafting fixed is put down gently one by one and trained in advance in the container that place mat damp filter paper, 80 85 degree of humidity is pre- to train 35 days, and the callus of scion and stock is combined substantially, and scion starts to reveal young leaves, is transplanted to nursery in the matrix of seedling-raising cup；4th step, field planting：When nursery grows 2 true leaves in 10 15 days, choosing is at dusk or grafting field planting is arrived field growing by the cloudy day, and soil often keeps moistening；The present invention provides new way to increase substantially rice yield.
Description
Technical field
The present invention relates in arviculture field, the distant hybridization method of particularly a kind of corn and paddy rice.
Background technology
Corn and paddy rice belong to gramineous herbaceous plant together, and corn returns Zea, and paddy rice is Oryza, and two kinds of crops are equal not
Belong to together, both graftings and hybridization belong to remote edge and combined, and have suitable technical difficulty.
What nineteen fifty-one published《Darwin complete or collected works》In point out：" asexual hybridization is exactly factor of the finger-type into new things, not necessarily
To be formed in female or male sexual organ, they can also be combined in cell tissue in no sexual organ, so as to produce
The former base of the sprouting of shape with two parental forms "；What nineteen fifty-five published《Meter Qiu Lin complete or collected works》Describe meter Qiu Lin all one's life
Create more than 500 new varieties, he using mentor method solve distant hybridization difficulty, using asexual and sexual distant hybridization as
One of means with new hereditary basis plant are obtained, a large amount of new varieties are cultivated；On June 22nd, 2009《Agricultural technology weekly》
Report is delivered and U.S. scientific magazine《Science》Achievement in research：Grafting can exchange hereditary information.This paper is Germany
Scientist uses fluorized marking gene technology in tobacco grafting experiment, it was demonstrated that the thing of the frequent producer exchange of grafting part
It is real, and propose a viewpoint " grafting is the obstacle of gene spans different plant species and a kind of new way for freely shifting "；In addition,
《Science of heredity》(Higher Education Publishing House, 1991, Liu Zudong write),《Crop Genetic Breeding knowledge》(Science and Technology of Shanghai publishing house,
1979, child one in write),《Medical cell biology》(2004, People's Health Publisher, Song Jindan etc. writes),《Plant
Learn》(2004, Science and Technology of Shanghai publishing house, Li Yanghan writes) and《Concise agricultural dictionary genetic breeding fascicle》(, science in 1979
Publishing house) etc. document hereditary information is had been described.
According to our retrieval, phase nineteen fifty-nine 01《Biology is circulated a notice of》One entitled《The few virtue of surprising invention Jiang is cultivated into
Work(new rice variety》In mention：" early in nineteen fifty-five, he carries out asexual hybridization (grafting) using corn and paddy rice ... ... cultivates
New kind, this chi of hybrid plant height 5 is more, and tiller is up to more than 100, and the chi of spike length one, per fringe 500, opens monocotyledon grafting
New way ".But simply there is the description of character property its hybrid plant feature in the document, do not refer to specific asexual miscellaneous
(grafting) method of friendship.In addition, there is not been reported for related technology.
The content of the invention
It is an object of the invention to provide a kind of corn and the distant hybridization method of paddy rice, strong adaptability, pest and disease damage are cultivated
Less, the compact new rice variety of plant type, by the high unique physiological dominance of four-carbon plant (corn) light respiration low intensity, photosynthetic efficiency
It is fused to c3 plants (paddy rice) to come up, so that the yield of the important grain such as paddy rice is greatly improved.
The purpose of the present invention is realized according to following technical proposals：
A kind of corn and the distant hybridization method of paddy rice, are divided into Juvenile stage pretreatment, grafting, nursery and four steps of field planting,
The first step, Juvenile stage adapts to the kind of local environmental conditions, and pretreatment includes：
1) seed soaks：Clean corn, rice paddy seed will be sterilized to soak in water 20-25 hours；
2) vernalization：Vernalization in clean culture dish is placed on after seed swelling, temperature is maintained at 20-25 degree, treats that plumule is grown to
Prepare grafting during 1.5CM-2CM；
Second step, grafting：It is same oblique to cut paddy rice plumule and by the water cut by the oblique excision part of maize germ
Rice plumule is fitted to rapidly on maize germ otch, and scion is fitted with stock, and center is interspersed to fix label, tightens junction surface；
3rd step, nursery：The grafting fixed is put down gently one by one and trained in advance in the container that place mat damp filter paper, it is wet
80-85 degree is spent, pre- to train 3-5 days, the callus of scion and stock is combined substantially, and scion starts to reveal young leaves, is transplanted to nursery
Nursery in the matrix of cup；
4th step, field planting：When nursery grows 2 true leaves for 10-15 days, choosing is at dusk or the cloudy day gives birth to grafting field planting to field
It is long, soil conservation moistening.
Beneficial effects of the present invention are：
1) a kind of new corn and the distant hybridization method of paddy rice are provided；
2) method provided according to the present invention enables to the corn, rice hybrid plant obtained to survive and solid；
3) the corn, rice hybrid plant that the method provided according to the present invention is cultivated has obvious change paddy rice into upland rice, raw
Long to accelerate, the features such as development shifts to an earlier date, hybrid plant growth is just eared solid, far below the growth week of general paddy rice only more than 90 day
4 months phases (southern area) to 7 months (northern area) time；
4) the corn, rice hybrid plant that the method provided according to the present invention is cultivated has 3-4 plants of effective tillering, is significantly
Degree improves rice yield and provides practical basis；
5) the corn, rice hybrid plant that the method provided according to the present invention is cultivated is for it grows natural environment, and 2014
Year, Yinchuan Area autumn weather was sombre, and illumination is not enough, and pest and disease damage does not occur for the hybrid plant still robust growth, high to cultivate
Produce the adaptable paddy rice of high-quality or upland rice provides new approach；
6) the corn, rice hybrid plant that the method provided according to the present invention is cultivated, by the unique excellent of four-carbon plant (corn)
Gesture is fused to c3 plants (paddy rice) and come up, and practical basis is provided for the yield that increases substantially the important grain such as paddy rice；
Brief description of the drawings
Fig. 1 is completes the corn, rice plumule photo of grafting, wherein through plumule, laterally disposed for acupuncture needle, under acupuncture needle
Side is paddy rice plumule, and top is maize germ, radicle and seed；
Fig. 2 is the paddy rice for bearing plumule；
Fig. 3 is the corn for bearing plumule；
Fig. 4 is grafting paddy rice plumule；
Fig. 5 is to cut off the corn parent used after plumule so as to grafting；
Fig. 6 is the corn, rice shoot of grafting survival；
Fig. 7 is the corn, rice plant of normal growth after grafting；
Fig. 8 is corn, rice plant solid after grafting；
Fig. 9 is the corn, rice complete stool that harvests after ripe.
Embodiment
The first step, first has to the parent that selection adapts to local environmental conditions：
1. stock and the selection of scion：
The selection of 1.1 stock corns：During distant grafting, mainly supply scion nutriment by stock and exchange hereditary information
To influence scion, therefore, stock should select the kind of genetic stability, preferably adapt to the purebred of local environment.
The selection of 1.2 scion paddy rice：It should select that genetic flexibility is big, conservative is small, ontogeny time short paddy rice children
Bud makees scion, is readily obtained remote edge asexual hybrid or the big breeding resources of variation, you should pay special attention to from joint portion and cultivate scion children
Bud.
In 1.3 the present embodiment, " simon 5 " is used in corn parent selection；" peaceful round-grained rice 43 " is used in parental rice selection.
2. parent pre-processes：
2.1 seeds soak：Garbled parent (corn and paddy rice) seed disinfection is clean, 20- is soaked in water afterwards
25 hours, the specific time depending on actual room temperature, seed swelling.
2.2 vernalization：The corn and rice paddy seed of swelling are placed on vernalization in clean culture vessel, temperature is maintained at 20-
25 degree, grafting when plumule grows to 1.5CM-2.0CM, the paddy rice and corn for bearing plumule is distinguished as shown in Figures 2 and 3.
Second step, the technical program and skill of distant grafting：
3. program：The oblique cut-out plumule of stock → from paddy rice is oblique to be cut plumule and does scion → stock and cut with scion
Mouth laminating → laterally interspersed fixation is signed for joint portion → tightens joint portion.
3.1 grafting skills：When cutting off stock and scion plumule, grafting：By the oblique excision part of maize germ, such as Fig. 5
It is shown, it is equally oblique to cut paddy rice plumule and by the paddy rice plumule cut, the paddy rice plumule shown in Fig. 4 is fitted into rapidly corn
On plumule otch, after stock is fitted rapidly with scion plumule, intert acupuncture thin acupuncture needle at scion center of being fitted with stock.
The purpose of oblique excision plumule is to increase stock and the fitting area of scion；Stock is tightened with thin acupuncture needle and scion connects
Conjunction portion, the purpose tightened is to make stock and scion while produce minimally invasive, to make stock and the scion hereditary information in agglutination
More preferably exchanged.
The corn, rice seedling that grafting is completed is as shown in Figure 1.
3rd step, nursery：
Trained in advance in the container that place mat damp filter paper 4. a grafting fixed is put down gently one by one, humidity 80-85 degree,
Pre- training 3-5 days.
4.1 explanation on nursery：The grafting fixed is put down gently in place mat the container of damp filter paper one by one
Pre- training, humidity 80-85 degree, pre- training 3-5 days, now, the callus of scion and stock is combined substantially, and scion starts to reveal new
Leaf, is transplanted to nursery in the matrix of seedling-raising cup by the grafting shoot for revealing young leaves afterwards；
4th step, field planting：
5. nursery grows 2 true leaves for 10-15 days, choosing is at dusk or grafting field planting is arrived field growing by the cloudy day, and soil is protected
Moistening is held, now, corn, rice grafting shoot can be with normal growth, as shown in Figure 6.
It is follow-up and to carry out observed and recorded there is still a need for carry out careful field management, according to it is conventional note humidity, temperature and
Illumination condition, the callus that forming layer is grown is easy to dehydration and is dried and makes the withered death of hybrid plant, it is necessary to loosened the soil,
The associative operation such as water, apply fertilizer, until hybrid plant growth (as shown in Figure 7), maturation (as shown in Figure 8), grafting plant is last
Harvesting corn paddy rice cross breeding seed (as shown in Figure 9).
So far, the present embodiment is completed.
Claims (1)
1. a kind of corn and the distant hybridization method of paddy rice, it is characterised in that：Be divided into Juvenile stage pretreatment, grafting, nursery with
It is colonized four steps：
The first step, Juvenile stage adapts to the kind of local environmental conditions, and pretreatment includes：
1) seed soaks：Clean corn, rice paddy seed will be sterilized to soak in water 20-25 hours；
2) vernalization：Vernalization in clean culture dish is placed on after seed swelling, temperature is maintained at 20-25 degree, treats that plumule is grown to
Prepare grafting during 1.5CM-2CM；
Second step, grafting：It is same oblique to cut paddy rice plumule and by the EMBRYO IN RICE cut by the oblique excision part of maize germ
Bud is fitted to rapidly on maize germ otch, and scion is fitted with stock, and center is interspersed to fix label, tightens junction surface；
3rd step, nursery：The grafting fixed is put down gently one by one and trained in advance in the container that place mat damp filter paper, humidity
The callus of 80-85 degree, pre- training 3-5 days, scion and stock is combined substantially, and scion starts to reveal young leaves, is transplanted to seedling-raising cup
Matrix in nursery；
4th step, field planting：When nursery grows 2 true leaves for 10-15 days, choosing is at dusk or grafting field planting is arrived field growing by the cloudy day,
Soil conservation is moistened.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410695545 | 2014-11-28 | ||
| CN2014106955459 | 2014-11-28 |
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| Publication Number | Publication Date |
|---|---|
| CN105706897A CN105706897A (en) | 2016-06-29 |
| CN105706897B true CN105706897B (en) | 2017-08-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410726900.4A Active CN105706897B (en) | 2014-11-28 | 2014-12-04 | Corn and the distant hybridization method of paddy rice |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201818578D0 (en) | 2018-11-14 | 2018-12-26 | Cambridge Entpr Ltd | Perennial monocotyledon grafting |
| GB201818577D0 (en) | 2018-11-14 | 2018-12-26 | Cambridge Entpr Ltd | Graft-mediated hybridisation of monocotyledonous plants |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1291429A (en) * | 2000-11-28 | 2001-04-18 | 程旭波 | Method for selectively breeding superhigh-dominance rice variety by distant suxual hybridization between rice and corn |
| CN1860855A (en) * | 2006-06-16 | 2006-11-15 | 程旭波 | Method for breeding corn-rice line-three with super-strong hybridization-dominating due to distant-hybridization by rice and corn |
| CN101473786A (en) * | 2009-02-17 | 2009-07-08 | 山东农业大学 | Method for breeding elite seed by distant hybridization of graminaceous plant |
| CN103155808A (en) * | 2011-12-15 | 2013-06-19 | 长江大学 | Conjoined grafting method for plant meristem |
-
2014
- 2014-12-04 CN CN201410726900.4A patent/CN105706897B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1291429A (en) * | 2000-11-28 | 2001-04-18 | 程旭波 | Method for selectively breeding superhigh-dominance rice variety by distant suxual hybridization between rice and corn |
| CN1860855A (en) * | 2006-06-16 | 2006-11-15 | 程旭波 | Method for breeding corn-rice line-three with super-strong hybridization-dominating due to distant-hybridization by rice and corn |
| CN101473786A (en) * | 2009-02-17 | 2009-07-08 | 山东农业大学 | Method for breeding elite seed by distant hybridization of graminaceous plant |
| CN103155808A (en) * | 2011-12-15 | 2013-06-19 | 长江大学 | Conjoined grafting method for plant meristem |
Non-Patent Citations (2)
| Title |
|---|
| 惊人的创举蒋少芳培育成功水稻新品种;蒋承组;《生物学通报》;19591231;第6页左栏第2段 * |
| 禾本科植物种芽嫁接法;李集临等;《生物学通报》;19600630;第249-262页，尤其是第249页第1段和右栏第2段，250页第左栏第5段至右栏第3段，以及图1、图2、图3 * |
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| CN105706897A (en) | 2016-06-29 |
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