CN104957039A - Rapid propagation and maintenance method for fittonia verschaffeltii - Google Patents
Rapid propagation and maintenance method for fittonia verschaffeltii Download PDFInfo
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Abstract
The invention belongs to the technical field of seedling propagation, and relates to a rapid propagation and maintenance method for fittonia verschaffeltii. The method comprises the steps of selecting a strong immature stem without disease and pest injury, removing leaves, cutting into single-bud stem segments being 0.8 to 1.2 cm in length after sterilization, after inserting the single-bud stem segments, with the buds being upward, into a culture medium to carry out clustered bud induction, carrying out ramet on clustered buds to perform clustered bud multiplication culture, after carrying out ramet on the cultured clustered buds, inserting into a culture solution for soaking for 10 minutes, then inserting into a mixed medium of perlite and turfy soil which are subjected to sterilization treatment, and transplanting after being cultured in the mixed medium of perlite and turfy soil for 30 days. According to the method, a manner that tissue culture is combined with cuttage is adopted for rapidly culturing the fittonia verschaffeltii, and the culture medium of the tissue culture is adjusted, so that the contamination rate of tissue culture seedlings is greatly decreased, the number of clustered bud induction is 3 to 5, the clustered bud propagation attachment times are more than 5, the number of branches of the propagated fittonia verschaffeltii is large, the cuttage rooting percentage reaches above 93 percent, the plant type is attractive, the ornamental value is improved, and the propagation cycle is short. According to the method, in an annual cycle, the number of the tissue culture propagation is above 8 times that of cutting propagation.
Description
Technical field
The present invention relates to the raising technology of a Plants, belong to seedling-wood breeding technical field, specifically, relate to a kind of Fast-propagation and maintenance process of reticulate pattern grass.
Background technology
Reticulate pattern grass belongs to Acanthaceae, is perennial evergreen herbaceous plant, originates in South America Peru.Its plant is short, and in crawling, shape spreads growth, and height is 5-20 centimetre about, and stipes of crawling easily is taken root; Leaf cross is to life, avette or oval, and cauline leaf, petiole, bennet are all close by fine hair, and the page gathers redness or white net arteries and veins, the raw spike in top, brightly yellowish look.
Reticulate pattern grass attitude is slim and graceful, and plant is small and exquisite, belongs to small potted plant plant in ornamental foliage plant.Because vein is clear, leaf look simple and elegant, and texture is well-balanced, have simultaneously resistance to dark and damp, like the features such as warm and easy cultivation, be loved by the people, be current American-European potted plant little basin kind all the fashion, be particularly suitable for potted plant viewing and admiring.The mode of reproduction of existing reticulate pattern grass is cottage propagation, division propagation and tissue culture propagation.
Cutting propagation: reticulate pattern grass is under appropriate temperature conditions, and the whole year can cottage propagation, best with cutting experiment when 5 ~ September, temperature was slightly high.Clip cutting from the stolon growing pelvic surface of sacrum, long 10 cm, generally need have 3 ~ 4 stipes, remove lower blade, slightly dry the husky bed of rear insertion.If plugging soil temperature is 24 ~ 30 DEG C time, within 7 ~ 14 days, can take root after inserting.If temperature is too low, rooting of cuttings is more difficult.Generally within latter 1 month, basin can be transplanted slotting.Plant division is bred: to the plant of cauline leaf growth fraction comparatively dense, has on stipes of much crawling and grows adventive root, as long as stolon is with root to cut more than 10 centimetres, all can be directly potted plant, and after Ban Yinchu recovers 1 ~ 2 week, proceed to normal maintenance.
Group training is bred: often make explant with blade and stem apex.Sterilization rear blade is cut into 8 ~ 10 millimeters long, is inoculated into MS medium and adds on 6-benzyl aminoadenine 2 mg/litre, methyl α-naphthyl acetate 2 mg/litre and 2,4-D1 mg/litre medium, and within 30 days, rear blade bends, and grows Multiple Buds after 20 days.Adding on methyl α-naphthyl acetate 0.1 mg/litre medium by moving into 1/2MS medium after Multiple Buds cutting, within about 1 ~ 2 week, growing adventive root, forming whole plant.
It is slow to there is speed of breeding in the careless mode of reproduction of above-mentioned existing reticulate pattern, and the demand branch quantity being difficult to meet market is few, and sight is low waits deficiency.
Summary of the invention
The present invention is directed to the above-mentioned deficiencies such as the speed of breeding that existing reticulate pattern grass mating system exists is slow, branch quantity is few, sight is low, provide a kind of Fast-propagation of reticulate pattern grass and the method for maintenance.The mode Fast-propagation reticulate pattern grass that the method utilizes method for tissue culture to combine with cuttage, and regulate the medium of tissue cultures, the pollution rate of plantlet in vitro is reduced greatly, and the reticulate pattern grass branch quantity of breeding is many, and cylindricality is attractive in appearance, and sight is high, and breeding cycle is short.
Technical scheme of the present invention is: a kind of Fast-propagation of reticulate pattern grass and maintenance process, comprise the following steps:
(1) draw materials: choose the healthy and strong tender stem without damage by disease and insect, removing blade, is cut into 0.8-1.2cm stem-segment with single bud after sterilizing.
(2) clump bud inducement: the bud head of the stem-segment with single bud obtained in step (1) is upwards inserted in medium and turns out clump bud.
(3) clump Shoot propagation is cultivated: by the plant division of clump bud or the stem section being cut into 0.8-1.2cm, is inoculated in the medium in step (2) and continues to cultivate.
(4) matrix is prepared: mixed by weight the ratio for 1:2 with turfy soil by perlite, and by liquor potassic permanganate, sterilization is carried out to it, after sterilization completes, with clear water, potassium permanganate is washed out rear acquisition matrix, for subsequent use.
(5) culture fluid is prepared: obtain culture fluid by after bactericide, root-growing agent and water in proportion Homogeneous phase mixing.
(6) soak: the clump bud after Multiplying culture in step (3) is put into culture fluid and soaks, soaking is highly 1/3 of clump bud.
(7) cuttage root-taking: cultivated in the matrix clump bud inserting step (4) after soaking prepared, length of embedment is 2cm, and temperature maintains 20 ± 1 DEG C.
(8) maintenance: the cuttage seeding shade net of 75% hides, and adopts page spray pattern, a day 1-2 time when watering.
(9) transplanting of cuttage branch: after cuttage branch cultivates 30d in matrix, can transplant it.
As preferably, in step (1), after the tender stem chosen removes blade, clean up with suds and banister brush stem section surface, then repeatedly 10min is rinsed with running water, aseptically carrying out sterilizing with the alcohol-pickled 25s of 70%-75%, by sterile water wash 2-3 time, then is the HgCl of 0.1% by concentration
2soak 8min and carry out sterilizing, then use aseptic water washing 6 times, cut off the part that stem section two ends contact with above-mentioned bactericidal agent, be cut into 0.8-1.2cm stem-segment with single bud.
As preferably, in step (2), condition of culture is: intensity of illumination is 15000-20000lx, and light application time is 12h/d, and temperature maintains 25 ± 2 DEG C.
Further, in step (2), the stem section that stem-segment with single bud cultivates 30 ± 2d rear section band stipes in the medium grows clump bud from eustipes part, and the stem section not with stipes after 40 ± 2d directly grows clump bud from blocking top.
As preferably, in step (2), the pulp furnish of described medium is: basal medium, sucrose 30g/L, agar 8g/L, 6-benzyl aminopurine 1mg/L, methyl α-naphthyl acetate 0.2mg/L, active carbon 0.5g/L.
Further, in step (3), cultivate most stem section after 25 days and start have lateral bud to grow.
As preferably, in step (4), in liquor potassic permanganate, the weight ratio of potassium permanganate and water is 1:800-1:1200.
As preferably, in step (6), the soak time of the clump bud after Multiplying culture in culture fluid is 10min, and the clump bud after described Multiplying culture is incubation time more than 3 months, highly reaches the clump bud of 5-8 centimetre.
As preferably, in step (5), in described culture fluid, the weight ratio of bactericide and water is 1:1000-3:1000, and the weight ratio of root-growing agent and water is 1:800, and make a living root powder and water of described root-growing agent mixes according to the ratio of weight ratio 1:800.
As preferably, in step (5), described bactericide is the tpn of 75% wetting powder, and described pulvis of taking root is fast-growing root.
Further, in step (7), cultivating 10 ± 2d can take root.
The invention has the beneficial effects as follows: the mode Fast-propagation reticulate pattern grass that the present invention adopts tissue cultures to combine with cuttage, and regulate the medium of tissue cultures, the pollution rate of plantlet in vitro is reduced greatly, quantity 3-5 bud of clump bud inducement, clump Shoot propagation is attached troops to a unit multiple more than 5 times, and the reticulate pattern grass branch amount of breeding is many, insert thousand rooting rate and reach more than 93%, column type is attractive in appearance, and sight improves, the cycle of breeding is short, and plant resistance against diseases is strong.The present invention is in annual period, and tissue culture propagation quantity can reach more than 8 times of cottage propagation.
Embodiment
Conveniently those skilled in the art understand, and below in conjunction with embodiment, the invention will be further described.
Embodiment 1:
The Fast-propagation of reticulate pattern grass and a maintenance process, it comprises the following steps:
(1) draw materials: choose the healthy and strong tender stem without damage by disease and insect, removing blade, clean up with suds and banister brush stem section surface, then repeatedly 10min is rinsed with running water, aseptically carry out sterilizing with the alcohol-pickled 25s of 73%, by sterile water wash 2 times, then be the HgCl of 0.1% by concentration
2soak 8min and carry out sterilizing, then use aseptic water washing 6 times, cut off the part that stem section two ends contact with above-mentioned bactericidal agent, be cut into 1.0cm stem-segment with single bud.
(2) clump bud inducement: the bud head of the stem-segment with single bud obtained in step (1) is upwards inserted in medium and turns out clump bud.Condition of culture is: intensity of illumination is 18000lx, and light application time is 12h/d, and temperature maintains 25 DEG C.The stem section that stem-segment with single bud cultivates 30d rear section band stipes in the medium grows clump bud from eustipes part, and the stem section not with stipes after 40d directly grows clump bud from blocking top.Wherein, the pulp furnish of described medium is: basal medium, sucrose 30g/L, agar 8g/L, 6-benzyl aminopurine 1mg/L, methyl α-naphthyl acetate 0.2mg/L, active carbon 0.5g/L.
(3) clump Shoot propagation is cultivated: will be cut into the stem section of 1.0cm after the plant division of clump bud, and be inoculated in the medium in step (2) and continue to cultivate, after 25 days, most stem section starts have lateral bud to grow.
(4) matrix is prepared: mixed by weight the ratio for 1:2 with turfy soil by perlite, and by the liquor potassic permanganate that the weight ratio of potassium permanganate and water is 1:1000, sterilization is carried out to it, with clear water, potassium permanganate is washed out rear acquisition matrix after sterilization completes, for subsequent use.
(5) culture fluid is prepared: obtain culture fluid by after bactericide, root-growing agent and water in proportion Homogeneous phase mixing.In described culture fluid, the weight ratio of bactericide and water is 2:1000, and the weight ratio of root-growing agent and water is 1:800, and make a living root powder and water of described root-growing agent mixes according to the ratio of weight ratio 1:800.Described bactericide is the tpn of 75% wetting powder, and described pulvis of taking root is fast-growing root.
(6) soak: the clump bud after Multiplying culture in step (3) is put into culture fluid and soaks, soaking is highly 1/3 of clump bud, soak time is 10min, and the clump bud after described Multiplying culture is incubation time more than 3 months, the clump bud highly reaching 5cm.
(7) cuttage root-taking: cultivated in the matrix clump bud inserting step (4) after soaking prepared, length of embedment is 2cm, and temperature maintains 20 DEG C, and cultivating 10d can take root.
(8) maintenance: the cuttage seeding shade net of 75% hides, and adopts the page spray pattern when watering, frequency of watering according to weather condition, one day 1-2 time.
(9) transplanting of cuttage branch: after cuttage branch cultivates 30d in matrix, can transplant it.
Test in Qingdao Hua Shenglvneng agricultural science and technology Co., Ltd group training indoor, environmental temperature is 25-28 DEG C, guarantees to have abundant water resources, irrigation method is proper, by said method breeding reticulate pattern grass, get 10 stem-segment with single bud, clump bud inducement obtains 42 buds in 40 days afterwards, obtains 42 strains, obtain clump bud plant division 200 strain after clump Shoot propagation after the plant division of clump bud, cuttage is carried out to 200 strain clump bud plant division, within 10 days, check 196 strains of taking root afterwards, transplant after 30 days, survive 192 strains.
Embodiment 2:
The Fast-propagation of reticulate pattern grass and a maintenance process, it comprises the following steps:
(1) draw materials: choose the healthy and strong tender stem without damage by disease and insect, removing blade, clean up with suds and banister brush stem section surface, then repeatedly 10min is rinsed with running water, aseptically carry out sterilizing with the alcohol-pickled 25s of 70%, by sterile water wash 2 times, then be the HgCl of 0.1% by concentration
2soak 8min and carry out sterilizing, then use aseptic water washing 6 times, cut off the part that stem section two ends contact with bactericidal agent, be cut into 0.8 stem-segment with single bud.
(2) clump bud inducement: the bud head of the stem-segment with single bud obtained in step (1) is upwards inserted in medium and turns out clump bud.Condition of culture is: intensity of illumination is 15000lx, and light application time is 12h/d, and temperature maintains 25 DEG C.The stem section that stem-segment with single bud cultivates 30d rear section band stipes in the medium grows clump bud from eustipes part, and the stem section not with stipes after 40d directly grows clump bud from blocking top.Wherein, the pulp furnish of described medium is: basal medium, sucrose 30g/L, agar 8g/L, 6-benzyl aminopurine 1mg/L, methyl α-naphthyl acetate 0.2mg/L, active carbon 0.5g/L.
(3) clump Shoot propagation is cultivated: will be cut into the stem section of 0.8-1.2cm after the plant division of clump bud, and be inoculated in the medium in step (2) and continue to cultivate, after 25 days, most stem section starts have lateral bud to grow.
(4) matrix is prepared: mixed by weight the ratio for 1:2 with turfy soil by perlite, and by the liquor potassic permanganate that the weight ratio of potassium permanganate and water is 1:800, sterilization is carried out to it, with clear water, potassium permanganate is washed out rear acquisition matrix after sterilization completes, for subsequent use.
(5) culture fluid is prepared: after bactericide, root-growing agent and water ratio uniform being mixed, obtain culture fluid.In described culture fluid, the weight ratio of bactericide and water is 1:1000, and the weight ratio of root-growing agent and water is 1:800, and make a living root powder and water of described root-growing agent mixes according to the ratio of weight ratio 1:800.Described bactericide is the tpn of 75% wetting powder, and described pulvis of taking root is fast-growing root.
(6) soak: the clump bud after Multiplying culture in step (3) is put into culture fluid and soaks, soaking is highly 1/3 of clump bud, soak time is 10min, and the clump bud after described Multiplying culture is incubation time more than 3 months, the clump bud highly reaching 6cm.
(7) cuttage root-taking: cultivated in the matrix clump bud inserting step (4) after soaking prepared, length of embedment is 2cm, and temperature maintains 19 DEG C, and cultivating 10d can take root.
(8) maintenance: the cuttage seeding shade net of 75% hides, and adopts the page spray pattern when watering, frequency of watering according to weather condition, one day 1-2 time.
(9) transplanting of cuttage branch: after cuttage branch cultivates 30d in matrix, can transplant it.
Test in Qingdao Hua Shenglvneng agricultural science and technology Co., Ltd group training indoor, environmental temperature is 25-28 DEG C, guarantees to have abundant water resources, irrigation method is proper, by said method breeding reticulate pattern grass, get 10 stem-segment with single bud, clump bud inducement obtains 37 buds in 38 days afterwards, obtains 37 strains, obtain clump bud plant division 150 strain after clump Shoot propagation after the plant division of clump bud, cuttage is carried out to 150 strain clump bud plant division, within 10 days, check 147 strains of taking root afterwards, transplant after 30 days, survive 142 strains.
Embodiment 3:
The Fast-propagation of reticulate pattern grass and a maintenance process, it comprises the following steps:
(1) draw materials: choose the healthy and strong tender stem without damage by disease and insect, removing blade, clean up with suds and banister brush stem section surface, then repeatedly 10min is rinsed with running water, aseptically carry out sterilizing with the alcohol-pickled 25s of 75%, by sterile water wash 2 times, then be the HgCl of 0.1% by concentration
2soak 8min and carry out sterilizing, then use aseptic water washing 6 times, cut off the part that stem section two ends contact with bactericidal agent, be cut into 1.2cm stem-segment with single bud.
(2) clump bud inducement: the bud head of the stem-segment with single bud obtained in step (1) is upwards inserted in medium and turns out clump bud.Condition of culture is: intensity of illumination is 20000lx, and light application time is 12h/d, and temperature maintains 25 DEG C.The stem section that stem-segment with single bud cultivates 30d rear section band stipes in the medium grows clump bud from eustipes part, and the stem section not with stipes after 40d directly grows clump bud from blocking top.Wherein, the pulp furnish of described medium is: basal medium, sucrose 30g/L, agar 8g/L, 6-benzyl aminopurine 1mg/L, methyl α-naphthyl acetate 0.2mg/L, active carbon 0.5g/L.
(3) clump Shoot propagation is cultivated: will be cut into the stem section of 1.2cm after the plant division of clump bud, and be inoculated in the medium in step (2) and continue to cultivate, after 25 days, most stem section starts have lateral bud to grow.
(4) matrix is prepared: mixed by weight the ratio for 1:2 with turfy soil by perlite, and by the liquor potassic permanganate that the weight ratio of potassium permanganate and water is 1:1200, sterilization is carried out to it, with clear water, potassium permanganate is washed out rear acquisition matrix after sterilization completes, for subsequent use.
(5) culture fluid is prepared: obtain culture fluid by after bactericide, root-growing agent and water in proportion Homogeneous phase mixing.In described culture fluid, the weight ratio of bactericide and water is 3:1000, and the weight ratio of root-growing agent and water is 1:800, and make a living root powder and water of described root-growing agent mixes according to the ratio of weight ratio 1:800.Described bactericide is the tpn of 75% wetting powder, and described pulvis of taking root is fast-growing root.
(6) soak: the clump bud after Multiplying culture in step (3) is put into culture fluid and soaks, soaking is highly 1/3 of clump bud, soak time is 10min, and the clump bud after described Multiplying culture is incubation time more than 3 months, the clump bud highly reaching 7cm.
(7) cuttage root-taking: cultivated in the matrix clump bud inserting step (4) after soaking prepared, length of embedment is 2cm, and temperature maintains 21 DEG C, and cultivating 10d can take root.
(8) maintenance: the cuttage seeding shade net of 75% hides, and adopts foliage spray mode when watering, frequency of watering according to weather condition, one day 1-2 time.
(9) transplanting of cuttage branch: after cuttage branch cultivates 30d in matrix, can transplant it.
Test in Qingdao Hua Shenglvneng agricultural science and technology Co., Ltd group training indoor, environmental temperature is 25-28 DEG C, guarantees to have abundant water resources, irrigation method is proper,
Test in Qingdao Pu Dongzhen mono-peasant household green house, environmental temperature is 25-28 DEG C, guarantees to have abundant water resources, irrigation method is proper, by said method breeding reticulate pattern grass, get 10 stem-segment with single bud, clump bud inducement obtains 46 buds in 42 days afterwards, obtains 46 strains, obtain clump bud plant division 220 strain after clump Shoot propagation after the plant division of clump bud, cuttage is carried out to 220 strain clump bud plant division, within 10 days, check 211 strains of taking root afterwards, transplant after 30 days, survive 207 strains.
Environmental temperature is identical, guarantees to have abundant water resources, irrigation method is proper, get the stem-segment with single bud of same number, and it is as shown in the table that the clump bud plant division quantity obtained by Fast-propagation of the present invention and maintenance process and existing group train the clump bud plant division quantity that method obtains:
| Stem-segment with single bud number | The clump bud plant division number that the inventive method obtains | The clump bud plant division number that existing group of training method obtains |
| 10 | 153 | 22 |
Clump bud cuttage after being bred by clump bud inducement from maintenance process by Fast-propagation of the present invention is in different matrix, and its situation of taking root is as shown in the table:
| Matrix | Clump bud plant division (rooting rate/%) after the increment of clump bud |
| River sand | 55 |
| Perlite | 68 |
| Turfy soil | 74 |
| Perlite: turfy soil=1:2 | 96 |
As extension of the present invention, the clump bud in step (7) can cuttage in view basin, be directly used in and view and admire.
Above illustrated embodiment is only with illustrating the present invention for convenience, and at technical scheme category of the present invention, person of ordinary skill in the field does various simple deformation and modification, all should be included in above claim.
Claims (10)
1. the Fast-propagation of reticulate pattern grass and a maintenance process, is characterized in that: comprise the following steps:
(1) draw materials: choose the healthy and strong tender stem without damage by disease and insect, removing blade, is cut into 0.8-1.2cm stem-segment with single bud after sterilizing;
(2) clump bud inducement: the bud head of the stem-segment with single bud obtained in step (1) is upwards inserted in medium and turns out clump bud;
(3) clump Shoot propagation is cultivated: by the plant division of clump bud or the stem section being cut into 0.8-1.2cm, is inoculated in the medium in step (2) and continues to cultivate;
(4) matrix is prepared: mixed by weight the ratio for 1:2 with turfy soil by perlite, and by liquor potassic permanganate, sterilization is carried out to it, after sterilization completes, with clear water, potassium permanganate is washed out rear acquisition matrix, for subsequent use;
(5) culture fluid is prepared: obtain culture fluid by after bactericide, root-growing agent and water in proportion Homogeneous phase mixing;
(6) soak: the clump bud after Multiplying culture in step (3) is put into culture fluid and soaks, soaking is highly 1/3 of clump bud;
(7) cuttage root-taking: cultivated in the matrix clump bud inserting step (4) after soaking prepared, length of embedment is 2-3cm, and temperature maintains 20 ± 1 DEG C;
(8) maintenance: the cuttage seeding shade net of 75% hides, and adopts page spray pattern, a day 1-2 time when watering;
(9) transplanting of cuttage branch: after cuttage branch cultivates 30d in matrix, can transplant it.
2. the Fast-propagation of reticulate pattern grass according to claim 1 and maintenance process, it is characterized in that: in step (1), after the tender stem chosen removes blade, clean up with suds and banister brush stem section surface, then repeatedly 10min is rinsed with running water, aseptically carrying out sterilizing with the alcohol-pickled 25s of 70%-75%, by sterile water wash 2-3 time, then is the HgCl of 0.1% by concentration
2soak 8min and carry out sterilizing, then use aseptic water washing 6 times, cut off the part that stem section two ends contact with above-mentioned bactericidal agent, be cut into 0.8-1.2cm stem-segment with single bud.
3. the Fast-propagation of reticulate pattern grass according to claim 1 and maintenance process, it is characterized in that: in step (2), condition of culture is: intensity of illumination is 15000-20000lx, light application time is 12h/d, and temperature maintains 25 ± 2 DEG C.
4. the Fast-propagation of reticulate pattern grass according to claim 1 and maintenance process, it is characterized in that: in step (2), the stem section that stem-segment with single bud cultivates 30 ± 2d rear section band stipes in the medium grows clump bud from eustipes part, and the stem section not with stipes after 40 ± 2d directly grows clump bud from blocking top.
5. the Fast-propagation of the reticulate pattern grass according to claim 1 or 4 any one and maintenance process, it is characterized in that: in step (2), the pulp furnish of described medium is: basal medium, sucrose 30g/L, agar 8g/L, 6-benzyl aminopurine 1mg/L, methyl α-naphthyl acetate 0.2mg/L, active carbon 0.5g/L.
6. the Fast-propagation of reticulate pattern grass according to claim 1 and maintenance process, is characterized in that: in step (3), cultivate most stem section after 25 days and start have lateral bud to grow.
7. the Fast-propagation of reticulate pattern grass according to claim 1 and maintenance process, it is characterized in that: in step (4), in liquor potassic permanganate, the weight ratio of potassium permanganate and water is 1:800-1:1200.
8. the Fast-propagation of reticulate pattern grass according to claim 1 and maintenance process, it is characterized in that: in step (6), the soak time of clump bud after Multiplying culture in culture fluid is 10min, and the clump bud after described Multiplying culture is incubation time more than 3 months, highly reaches the clump bud of 5-8 centimetre.
9. the Fast-propagation of the reticulate pattern grass according to claim 1 or 8 any one and maintenance process, it is characterized in that: in step (5), in described culture fluid, the weight ratio of bactericide and water is 1:1000-3:1000, the weight ratio of root-growing agent and water is 1:800, and make a living root powder and water of described root-growing agent mixes according to the ratio of weight ratio 1:800.
10. the Fast-propagation of reticulate pattern grass according to claim 1 and maintenance process, is characterized in that: in step (7), and cultivating 10 ± 2d can take root.
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| CN113796185A (en) * | 2021-08-24 | 2021-12-17 | 北京市园林绿化集团有限公司 | Method for cultivating and maintaining saline-alkali tolerant plant suaeda salsa in south and culture solution thereof |
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| 杨玉想: "室内珍品网纹草的栽培与应用", 《河北林业科技》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106613824A (en) * | 2016-11-07 | 2017-05-10 | 王�华 | Banana plant division propagation method |
| CN112425385A (en) * | 2020-11-16 | 2021-03-02 | 广西南药康园投资有限责任公司 | Method for large-scale seedling culture of Adhatoda vasica |
| CN113796185A (en) * | 2021-08-24 | 2021-12-17 | 北京市园林绿化集团有限公司 | Method for cultivating and maintaining saline-alkali tolerant plant suaeda salsa in south and culture solution thereof |
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| CN104957039B (en) | 2017-05-17 |
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