CN113796185A - Method for cultivating and maintaining saline-alkali tolerant plant suaeda salsa in south and culture solution thereof - Google Patents
Method for cultivating and maintaining saline-alkali tolerant plant suaeda salsa in south and culture solution thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C5/00—Fertilisers containing other nitrates
- C05C5/02—Fertilisers containing other nitrates containing sodium or potassium nitrate
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
Abstract
The application relates to the field of suaeda salsa cultivation, in particular to a method for cultivating and maintaining saline-alkali tolerant plants suaeda salsa in the south and a culture solution thereof, and specifically discloses a method for cultivating and maintaining saline-alkali tolerant plants suaeda salsa in the south, which comprises the following steps: s1, placing the suaeda salsa seeds into the culture solution for culture to prepare culture buds; each kilogram of culture solution is prepared from the following raw materials in parts by weight: gibberellic acid, chitosan, benayl aminopurine and trace element mixture, and the balance of water; the trace element mixture comprises calcium nitrate, potassium nitrate and sodium molybdate, and the mass ratio of the calcium nitrate to the potassium nitrate to the sodium molybdate is 1 (1-2) to 1: 1.5; s2, selecting a culture bud, cutting the culture bud into a culture medium, and culturing to obtain a propagation bud; and S3, transplanting the propagation buds to outdoor cutting soil, and watering and cultivating. The preparation method has the advantage of improving the germination survival rate of the southern suaeda glauca bge.
Description
Technical Field
The application relates to the field of suaeda salsa cultivation, in particular to a cultivation and maintenance method of saline-alkali tolerant plants suaeda salsa in south and a culture solution thereof.
Background
Southern suaeda glauca is an annual herbaceous plant of the genus suaeda of the family chenopodiaceae, and is produced in places such as Guangdong, Guangxi, Fujian, Taiwan, Jiangsu, oceanic continents and Japan. Southern suaeda glauca usually grows in sand of beach, edge of mangrove and the like, and is generally grown in pieces; southern suaeda glauca has obvious advantages in the aspects of plant characteristics, suitable planting areas, growth speed, landscape effect and ecological restoration value, so that southern suaeda glauca is widely planted and cultivated in various projects such as urban landscape building, coastal wetland ecological restoration engineering, construction of coastal shelter forest mangrove systems and the like in coastal regions in south China.
The application publication No. CN111373995A discloses a planting method and application of suaeda salsa on coastal beach saline land, comprising the following steps of selecting areas suitable for planting in a tidal zone and a high-tide zone in an intertidal zone, and constructing a wave-resisting zone; step two, seeding, namely selecting temperature, seeding quantity and seeding time; and step three, managing after sowing, and covering the net, draining water and preventing flooding.
With respect to the above-described related art, the inventors consider that: in the suaeda salsa planting method, the suaeda salsa seeds are directly planted in the intertidal zone, so that the germination survival rate of the suaeda salsa is not high, the planting method is also suitable for cultivating the southern suaeda salsa, and the germination survival rate of the southern suaeda salsa is also required to be improved.
Disclosure of Invention
In order to improve the germination survival rate of suaeda salsa in south, the application provides a cultivation and maintenance method of saline-alkali tolerant plants suaeda salsa in south and a culture solution thereof.
In a first aspect, the application provides a method for cultivating and maintaining a saline-alkali tolerant plant, namely suaeda salsa, which adopts the following technical scheme:
a method for cultivating and maintaining saline-alkali tolerant plants, namely suaeda salsa in south comprises the following steps:
s1, placing the suaeda salsa seeds into the culture solution to be cultured for 5-6 days to prepare cultured buds; each kilogram of the culture solution is prepared from the following raw materials by weight: gibberellic acid 50-100mg, chitosan 60-120mg, benayl aminopurine 5-10mg, trace element mixture 8-15mg and the balance of water; the trace element mixture comprises calcium nitrate, potassium nitrate and sodium molybdate, and the mass ratio of the calcium nitrate to the potassium nitrate to the sodium molybdate is 1 (1-2) to 1-1.5;
s2, selecting a stem section with buds at the top of the culture bud prepared in the step S1, and cutting the stem section with buds into a culture medium for cultivation to obtain a propagation bud;
and S3, transplanting the propagation bud obtained in the step S2 into cutting soil located outdoors, and watering and cultivating.
By adopting the technical scheme, as the culture solution is prepared by adopting the gibberellic acid, the chitosan, the benzylaminopurine and the trace element mixture together, the content of the auxin in the seeds can be increased by putting the southern suaeda glauca seeds into the culture solution for culture, so that the auxin promotes the elongation, division and expansion of cells in the seeds, breaks the dormant period of the seeds and promotes the germination of the seeds; the seed is easy to be damaged by fungi in the germination process, the chitosan added in the culture solution can activate an immune system inside the seed, the plant has a distinguishing system so that the plant can quickly identify the chitosan and generate a large amount of chitin enzymes, and the chitosan is used as a main component of a fungal cell wall; after the seeds germinate, the addition of benzylaminopurine can inhibit the aging of the germinated buds of the seeds, so that the germination survival rate of the seeds is further improved; meanwhile, as the seeds involve a large amount of division and elongation of cells in the germination process, the addition of the calcium nitrate provides water-soluble nitrate nitrogen and quick-acting calcium for the seeds, which is beneficial to the germination and growth of the seeds; potassium nitrate provides potassium ions for the seeds, and the potassium ions can promote photosynthesis and carbohydrate synthesis and transportation in the seeds, so that the germination rate of the seeds is improved; the seeds need a large amount of protein in the germination process, the addition of sodium molybdate promotes the generation of nitrate reductase in the seeds, and the nitrate reductase can promote the synthesis of the protein in the seeds, accelerate the germination and growth of the seeds and improve the survival rate of the seeds; under the synergistic effect of gibberellic acid, chitosan, benzylaminopurine and trace element mixture, the germination survival rate of the southern suaeda glauca is improved.
Preferably, the suaeda glauca seeds in the step S1 are pre-treated before being placed in the culture solution, and the pre-treatment comprises the following steps:
1) airing the southern suaeda glauca seeds for 48-72 h;
2) placing the southern suaeda glauca seeds aired in the step 1) under an ultraviolet lamp to irradiate for 2-3 h;
3) and placing the southern suaeda glauca seeds irradiated by the ultraviolet lamp in the step 2) in water at the temperature of 20-30 ℃ for soaking for 3 hours, and taking out for later use.
By adopting the technical scheme, the southern suaeda glauca seeds are aired, so that the air permeability of the seed coats of the southern suaeda glauca seeds is improved, harmful respiration products in the seeds can be discharged, and the subsequent liquid absorption capacity of the seeds is enhanced; a large amount of germs are attached to the surfaces of the seeds, and the surfaces of the southern suaeda glauca seeds are disinfected and sterilized by ultraviolet rays, so that the invasion of the germs in the germination process of the seeds is reduced, and the germination rate of the seeds is improved; then the seeds of the southern common seepweed herb are soaked in water to soften the seed coats of the southern common seepweed herb, which is beneficial to the subsequent germination and sprouting.
Preferably, before the culture buds are cut into the culture medium in the step S1, naphthylacetic acid and indoleacetic acid are added into the culture medium, and the mass ratio of the culture medium, the naphthylacetic acid and the indoleacetic acid is 500 (1-1.5) to (1-2).
By adopting the technical scheme, the naphthylacetic acid is added into the culture medium, and can enter the culture buds and be transported to the parts with vigorous growth in the culture buds to promote the growth of the culture buds; meanwhile, the naphthylacetic acid can also promote the division and expansion of cells in the cultured buds, stimulate the division and differentiation of callus, induce the generation of root primordium and further promote the rooting process of the cultured buds; but the naphthylacetic acid has stronger effect of promoting rooting on plants and higher toxicity, and is easy to cause environmental pollution when being used in large quantity; the effect of the heteroauxin on promoting rooting of plants is weaker than that of the naphthylacetic acid, but the heteroauxin has lower toxicity and almost no pollution to the environment, and the common mixing effect of the naphthylacetic acid and the heteroauxin accelerates the rooting process of the cultured buds and reduces the pollution to the environment.
Preferably, after naphthylacetic acid and indoleacetic acid are added into the culture medium, lanthanum carbonate and brassin are added into the culture medium, and the mass ratio of the culture medium to the lanthanum carbonate to the brassin is 500:1 (2-3).
By adopting the technical scheme, after the bud is induced and cultured to root by naphthylacetic acid and indoleacetic acid, the brassin is added into the culture medium and is used as a growth regulator of plants, so that the root growth of the cultured bud can be promoted, and the root system of the cultured bud is more robust; a large amount of nutrient elements need to be absorbed in the growth process of the cultured bud root system, and lanthanum carbonate can enhance the absorption capacity of the cultured bud root system to the nutrient elements, promote the growth and development of the plant root system and improve the activity of the root system; the survival rate of the southern suaeda glauca is further improved through the combined action of the lanthanum carbonate and the brassin.
Preferably, the propagation bud is sterilized before being transplanted to the cutting soil, and the sterilization treatment comprises the following steps:
s11, wiping the propagation buds with a sodium hypochlorite solution, and then washing the propagation buds with water to obtain pre-sterilized buds;
s12, wiping the pre-sterilized bud obtained in the step S11 with alcohol solution, then washing with water, and sucking water for later use.
By adopting the technical scheme, the hypochlorous acid is formed by hydrolyzing the sodium hypochlorite, and is further decomposed to generate nascent oxygen with strong oxidizability, and the nascent oxygen denatures proteins in the viruses on the surfaces of the propagating buds so as to kill the viruses on the surfaces of the propagating buds; meanwhile, because the alcohol has stronger volatility, the alcohol molecules can absorb the moisture in the protein on the surface of the propagation bud, so that the bacterial protein is dehydrated and solidified, thereby achieving the effect of sterilizing the propagation bud; the method has the advantages that the breeding buds are sterilized and disinfected under the combined action of the alcohol solution and the sodium hypochlorite solution, and the survival rate of breeding bud cultivation is improved.
Preferably, before the propagation buds are transplanted into the cutting soil in the step S3, turfy soil and vermiculite are added into the cutting soil and are uniformly turned, and the mass ratio of the cutting soil to the turfy soil to the vermiculite is 10:1: 1.
By adopting the technical scheme, the turfy soil is soft and has good air permeability, and meanwhile contains a large amount of organic matters and humic acid, so that a natural ventilating and organic slow-release cultivation environment can be formed; the breeding buds need a large amount of trace elements in the growth process, and the vermiculite contains potassium, magnesium, calcium, iron, trace elements such as manganese, copper, zinc and the like, and can provide the necessary trace elements for the breeding buds for a long time; therefore, the culture soil, the turfy soil and the vermiculite are mixed, so that the propagation buds have good survival rate after being cut into the cutting soil.
In a second aspect, the application provides a saline-alkali tolerant plant suaeda salsa culture solution, which adopts the following technical scheme:
a saline-alkali tolerant plant suaeda salsa south culture solution is prepared from the following raw materials in parts by weight: 50-100mg of gibberellic acid, 60-120mg of chitosan, 5-10mg of benzylaminopurine, 8-15mg of trace element mixture and the balance of water; the trace element mixture comprises calcium nitrate, potassium nitrate and sodium molybdate, and the mass ratio of the calcium nitrate to the potassium nitrate to the sodium molybdate is 1 (1-2) to 1-1.5.
By adopting the technical scheme, the gibberellin in the culture solution can improve the content of auxin in the southern common seepweed herb seeds, and the auxin promotes the elongation, division and expansion of cells in the southern common seepweed herb seeds, so that the dormancy period of the southern common seepweed herb seeds is broken, and the germination of the southern common seepweed herb seeds is promoted; the chitosan is added to activate the immune system inside the seeds, and the identification degree of the identification system of the seeds to the chitosan is improved, when fungi invade the seeds, the seeds can generate a large amount of chitin ferment to decompose the chitosan on the cell walls of the fungi after identifying the chitosan in the cell walls of the fungi, thereby achieving the antibacterial effect and improving the survival rate of the seeds; when the southern suaeda glauca seeds germinate, the benayl aminopurine can inhibit the aging of the germinated seeds, so that the survival rate of the seeds is further improved; the addition of the calcium nitrate provides water-soluble nitrate nitrogen and quick-acting calcium for the plant seeds, which is beneficial to the germination and growth of the seeds of the southern suaeda glauca; the potassium nitrate can promote the absorption of nitrogen and potassium by the seeds, further promote the photosynthesis in the seeds and the synthesis and transportation of carbohydrates, and improve the survival rate of the southern suaeda salsa seeds; the molybdenum element provides molybdenum element for the seeds, the molybdenum element is beneficial to the generation of nitrate reductase in the seeds, the nitrate reductase can promote the synthesis of protein in the seeds, a large amount of protein is needed in the germination and growth process of the seeds, so the germination and growth of the seeds can be accelerated, meanwhile, the molybdenum element can also promote the generation of propagation organs of the seeds, the survival rate of the seeds is improved, and the germination survival rate of the southern suaeda glauca seeds is improved through the synergistic effect of the mixture of gibberellic acid, chitosan, benzylaminopurine and trace elements.
Preferably, each kilogram of the culture solution is prepared from the following raw materials by weight: 65-90mg of gibberellic acid, 70-100mg of chitosan, 6-9mg of benzylaminopurine, 10-12mg of trace element mixture and the balance of water; the trace element mixture comprises calcium nitrate, potassium nitrate and sodium molybdate, and the mass ratio of the calcium nitrate to the potassium nitrate to the sodium molybdate is 1 (1-2) to 1-1.5.
By adopting the technical scheme, the proportion of each raw material in the culture solution is optimized, the mutual proportion of the gibberellic acid, the chitosan, the benzylaminopurine and the trace element mixture in the culture solution is further adjusted, and the survival rate of the southern suaeda glauca seeds in the culture solution is improved.
In summary, the present application has the following beneficial effects:
according to the method, the southern suaeda glauca seeds are placed in the culture solution for culture, gibberellin in the culture solution can break the dormancy stage of the seeds, and the germination of the seeds is promoted; meanwhile, the added chitosan can resist the infection of fungi in the germination process of the seeds, and the influence of the fungal infection on the germination rate of the seeds is reduced; the addition of benzylaminopurine is used for inhibiting the aging of the germinated buds; the addition of the trace element mixture provides the required trace elements for the seed germination process, accelerates the germination process of the seeds, and improves the germination survival rate of the southern suaeda glauca seeds through the combined action of the gibberellic acid, the chitosan, the benzylaminopurine and the trace element mixture.
Detailed Description
The present application will be described in further detail with reference to examples.
In the following examples, it is preferable that gibberellic acid is produced by Hebei Danming Biotech limited, and the content of effective substances is 99%;
preferably, the chitosan is chitosan produced by Chengdu Tepu biotechnology, Inc., and the content of effective components is 0.5%;
preferably, the benzylaminopurine is benzylaminopurine produced by Hubei Jiujiulong GmbH, and the content of effective components is 2%;
preferably, the calcium nitrate used has a calcium content of greater than 16%;
preferably, the potassium nitrate is produced by short-time evolution chemical company Limited in Taiyuan city, and the purity is 99.6 percent
Preferably, the sodium molybdate is Bester molybdenum products, Inc. of Thai, and the molybdenum content is not lower than 39.3 percent;
preferably, the lanthanum carbonate is provided by Kai new materials Co., Ltd, Jining, and the purity is 99.0%;
preferably, the indoleacetic acid is provided by Shandong ChuanHao new material limited, and the purity is 98%;
preferably, the brassin is produced by Jinhaoded Biotechnology limited, and the content of effective components is 99.9%;
preferably, the concentration of sodium hypochlorite in the sodium hypochlorite solution is 0.055%, and the concentration of ethanol in the alcoholic solution is 75%;
potassium nitrate was included at 1900mg per kg of medium used in this application; 1650mg of ammonium nitrate; 170mg of monopotassium phosphate; 370mg of magnesium sulfate; 440mg of calcium chloride; potassium iodide 0.83 mg; boric acid 6.2 mg; manganese sulfate 22.3 mg; 8.6mg of zinc sulfate; 0.25mg of sodium molybdate; copper sulfate 0.025 mg; 0.025mg of cobalt chloride; 37.25mg of disodium ethylene diamine tetraacetate; 27.85mg of ferrous sulfate; 100mg of inositol; 2mg of glycine; thiamine hydrochloride 0.1 mg; pyridoxine hydrochloride 0.5 mg; nicotinic acid 0.5 mg; 30g of cane sugar; 7g of agar and the balance of water.
The cutting soil used in the application is soil with salt content of less than 1%; preferably, the turfy soil is provided by a autumn feather seedling substrate processing factory in Dongfeng county, and the content of organic matters in the turfy soil is 65-75%; preferably, the vermiculite used is vermiculite supplied by Asahi-Yangyi group of Hebei province, with a particle size of 30 mesh.
Preparation example
Culture solution preparation example 1, comprising the following steps:
1. respectively adding gibberellic acid 50mg, chitosan 60mg and benzylaminopurine 5mg into a stirring barrel with a stirring paddle, adding 999.877g of water into the stirring barrel, setting the stirring speed at 45r/min, and stirring at room temperature for 20min to prepare a first mixed solution;
2. adding 8mg of the trace element mixture into the first mixed solution prepared in the step 1, setting the stirring speed to be 30r/min, and stirring for 15min at room temperature to obtain the trace element compound; the trace element mixture is prepared from calcium nitrate, potassium nitrate and sodium molybdate according to the mass ratio of 1:1: 1.
The culture solution preparation example 2 includes the following steps:
1. respectively adding 100mg of gibberellic acid, 120mg of chitosan and 10mg of benzylaminopurine into a stirring barrel with a stirring paddle, adding 999.755g of water into the stirring barrel, setting the stirring speed at 45r/min, and stirring at room temperature for 20min to prepare a first mixed solution;
2. adding 15mg of the trace element mixture into the first mixed solution prepared in the step 1, setting the stirring speed to be 30r/min, and stirring for 15min to obtain the trace element compound; the trace element mixture is prepared from calcium nitrate, potassium nitrate and sodium molybdate according to the mass ratio of 1:1: 1.
Culture solution preparation example 3, comprising the following steps:
1. respectively adding 80mg of gibberellic acid, 90mg of chitosan and 8mg of benzylaminopurine into a stirring barrel with a stirring paddle, adding 999.811g of water into the stirring barrel, setting the stirring speed at 45r/min, and stirring at room temperature for 20min to prepare a first mixed solution;
2. and (2) adding 11mg of trace element mixture into the first mixed solution prepared in the step (1), setting the stirring speed to be 30r/min, and stirring at room temperature for 15min to obtain the trace element mixture, wherein the trace element mixture is prepared from calcium nitrate, potassium nitrate and sodium molybdate according to the mass ratio of 1:1: 1.
Culture solution preparation example 4, comprising the following steps:
this preparation example differs from preparation example 3 in that the trace element mixture added in step 2 was made of calcium nitrate, potassium nitrate and sodium molybdate in a mass ratio of 1:2: 1.5.
Culture solution preparation example 5, comprising the following steps:
this preparation example differs from preparation example 3 in that the trace element mixture added in step 2 was made of calcium nitrate, potassium nitrate and sodium molybdate in a mass ratio of 1:1.5: 1.25.
Example 1
A method for cultivating and maintaining saline-alkali tolerant plants, namely suaeda salsa in south comprises the following steps:
s1, taking 1kg of the culture solution prepared in the preparation example 1 of the culture solution, putting 500g of southern common seepweed herb seeds into the culture solution for immersion culture, and irradiating for 5 days under the conditions that the culture temperature is 25 ℃ and the illumination intensity is 2000lux, wherein the illumination time per day is 12 hours, so as to prepare culture buds;
s2, selecting 1-2 nodes of stem segments with buds at the top of the culture buds prepared in the step S1, cutting the stem segments with buds into 2kg of culture medium, setting the culture temperature at 25 ℃, the relative humidity at 50%, the illumination intensity at 1800lux, and the illumination time per day at 12h, and culturing for 14 days to prepare propagation buds;
and S3, transplanting the propagation bud prepared in the step S2 into cutting soil located outdoors, and watering and cultivating.
Example 2
A method for cultivating and maintaining saline-alkali tolerant plants, namely suaeda salsa in south comprises the following steps:
s1, taking 1kg of the culture solution prepared in the preparation example 1 of the culture solution, putting 500g of southern common seepweed herb seeds into the culture solution for immersion culture, and irradiating for 5 days under the conditions that the culture temperature is 25 ℃ and the illumination intensity is 2000lux, wherein the illumination time per day is 12 hours, so as to prepare culture buds;
s2, taking 2kg of culture medium, adding naphthylacetic acid and indoleacetic acid into 2kg of culture medium, wherein the mass ratio of the culture medium to the naphthylacetic acid to the indoleacetic acid is 500:1:1, and uniformly mixing to prepare a first culture medium;
s3, selecting 1-2 nodes of stem segments with buds at the top of the cultured bud prepared in the step S1, cutting the stem segments with buds into the first culture medium prepared in the step S2, setting the culture temperature to be 25 ℃, the relative humidity to be 50%, the illumination intensity to be 1800lux, the illumination time per day to be 12h, and culturing for 14 days to prepare a propagation bud;
s4, transplanting the propagation bud prepared in the step S3 into cutting soil located outdoors, and watering and cultivating.
Example 3
The method for cultivating and maintaining the saline-alkali tolerant plant, namely the suaeda salsa south, in the embodiment is different from the method in the embodiment 2 in that the first culture medium in the step S2 is prepared from a culture medium, naphthylacetic acid and indoleacetic acid according to the mass ratio of 500:1.5:2, and the rest is the same as the method in the embodiment 2.
Example 4
The method for cultivating and maintaining the saline-alkali tolerant plant, namely the suaeda salsa south, in the embodiment is different from the method in the embodiment 2 in that the first culture medium in the step S2 is prepared from a culture medium, naphthylacetic acid and indoleacetic acid according to the mass ratio of 500:1.25:1.5, and the rest is the same as that in the embodiment 2.
Example 5
A method for cultivating and maintaining saline-alkali tolerant plants, namely suaeda salsa in south comprises the following steps:
s1, taking 1kg of the culture solution prepared in the preparation example 1 of the culture solution, putting 500g of southern common seepweed herb seeds into the culture solution for immersion culture, and irradiating for 5 days under the conditions that the culture temperature is 25 ℃ and the illumination intensity is 2000lux, wherein the illumination time per day is 12 hours, so as to prepare culture buds;
s2, taking 2kg of culture medium, adding naphthylacetic acid and indoleacetic acid into 2kg of culture medium, wherein the mass ratio of the culture medium to the naphthylacetic acid to the indoleacetic acid is 500:1.25:1.5, and uniformly mixing to prepare a first culture medium; adding lanthanum carbonate and brassin into the first culture medium, uniformly mixing to prepare a second culture medium, wherein the second culture medium is prepared from the culture medium, naphthylacetic acid, indoleacetic acid, lanthanum carbonate and brassin according to the mass ratio of 500:1.25:1.5:1: 2;
s3, selecting 1-2 nodes of stem segments with buds at the top of the cultured bud prepared in the step S1, cutting the stem segments with buds into the second culture medium prepared in the step S2, setting the culture temperature to be 25 ℃, the relative humidity to be 50%, the illumination intensity to be 1800lux, the illumination time per day to be 12h, and culturing for 14 days to prepare a propagation bud;
s4, transplanting the propagation bud prepared in the step S3 into cutting soil located outdoors, and watering and cultivating.
Example 6
The method for cultivating and maintaining the saline-alkali tolerant plant suaeda salsa south of the embodiment is different from the method of the embodiment 3 in that the second culture medium prepared in the step S2 is prepared by a culture medium, naphthylacetic acid, indoleacetic acid, lanthanum carbonate and brassin according to the mass ratio of 500:1.25:1.5:1:3, and the rest is the same as that of the embodiment 5.
Example 7
A method for cultivating and maintaining saline-alkali tolerant plants, namely suaeda salsa in south comprises the following steps:
s1, airing 500g of southern suaeda glauca seeds outdoors for 48 hours;
s2, placing the southern suaeda glauca seeds aired in the step S1 under an ultraviolet lamp for irradiating for 2 hours;
s3, soaking the southern suaeda glauca seeds irradiated in the step S2 in water at the temperature of 20 ℃ for 3 hours to prepare primary treated seeds;
s4, taking 1kg of the culture solution prepared in the preparation example 1 of the culture solution, putting the primary treated seed obtained in the step S3 into the culture solution for immersion culture, and irradiating for 5 days under the conditions that the culture temperature is 25 ℃ and the illumination intensity is 2000lux, wherein the illumination time per day is 12h, so as to prepare a culture bud;
s5, taking 2kg of culture medium, adding naphthylacetic acid and indoleacetic acid into 2kg of culture medium, wherein the mass ratio of the culture medium to the naphthylacetic acid to the indoleacetic acid is 500:1.25:1.5, and uniformly mixing to prepare a first culture medium; adding lanthanum carbonate and brassin into the first culture medium, and uniformly mixing to prepare a second culture medium, wherein the second culture medium is prepared from the culture medium, naphthylacetic acid, indoleacetic acid, lanthanum carbonate and brassin according to the mass ratio of 500:1.25:1.5:1: 2;
s6, selecting 1-2 nodes of stem segments with buds at the top of the cultured bud prepared in the step S4, cutting the stem segments with buds into the second culture medium prepared in the step S5, setting the culture temperature to be 25 ℃, the relative humidity to be 50%, the illumination intensity to be 1800lux, the illumination time per day to be 12h, and culturing for 14 days to prepare a propagation bud;
s7, transplanting the propagation bud prepared in the step S6 into cutting soil located outdoors, and watering and cultivating.
Example 8
A method for cultivating and maintaining saline-alkali tolerant plants, namely suaeda salsa in south comprises the following steps:
s1, airing 500g of southern suaeda glauca seeds for 72 hours;
s2, placing the southern suaeda glauca seeds aired in the step S1 under an ultraviolet lamp for irradiating for 3 hours;
s3, soaking the southern suaeda glauca seeds aired in the step S2 in water at 30 ℃ for 3 hours to prepare primary treated seeds;
s4, taking 1kg of the culture solution prepared in the preparation example 1 of the culture solution, putting the primary treated seed obtained in the step S3 into the culture solution for immersion culture, and irradiating for 5 days under the conditions that the culture temperature is 25 ℃ and the illumination intensity is 2000lux, wherein the illumination time per day is 12h, so as to prepare a culture bud;
s5, taking 2kg of culture medium, adding naphthylacetic acid and indoleacetic acid into 2kg of culture medium, wherein the mass ratio of the culture medium to the naphthylacetic acid to the indoleacetic acid is 500:1.25:1.5, and uniformly mixing to prepare a first culture medium; adding lanthanum carbonate and brassin into the first culture medium, and uniformly mixing to prepare a second culture medium, wherein the second culture medium is prepared from the culture medium, naphthylacetic acid, indoleacetic acid, lanthanum carbonate and brassin according to the mass ratio of 500:1.25:1.5:1: 2;
s6, selecting 1-2 nodes of stem segments with buds at the top of the cultured bud prepared in the step S4, cutting the stem segments with buds into the second culture medium prepared in the step S5, setting the culture temperature to be 25 ℃, the relative humidity to be 50%, the illumination intensity to be 1800lux, the illumination time per day to be 12h, and culturing for 14 days to prepare a propagation bud;
s7, transplanting the propagation bud prepared in the step S6 into cutting soil located outdoors, and watering and cultivating.
Example 9
A method for cultivating and maintaining saline-alkali tolerant plants, namely suaeda salsa in south comprises the following steps:
s1, airing 500g of southern suaeda glauca seeds for 54 h;
s2, placing the southern suaeda glauca seeds aired in the step S1 under an ultraviolet lamp to irradiate for 2.5 hours;
s3, soaking the southern suaeda glauca seeds aired in the step S2 in water at 25 ℃ for 3 hours to prepare primary treated seeds;
s4, taking 1kg of the culture solution prepared in the preparation example 1 of the culture solution, putting the primary treated seed obtained in the step S3 into the culture solution for immersion culture, and irradiating for 5 days under the conditions that the culture temperature is 25 ℃ and the illumination intensity is 2000lux, wherein the illumination time per day is 12h, so as to prepare a culture bud;
s5, taking 2kg of culture medium, adding naphthylacetic acid and indoleacetic acid into 2kg of culture medium, wherein the mass ratio of the culture medium to the naphthylacetic acid to the indoleacetic acid is 500:1.25:1.5, and uniformly mixing to prepare a first culture medium; adding lanthanum carbonate and brassin into the first culture medium, and uniformly mixing to prepare a second culture medium, wherein the second culture medium is prepared from the culture medium, naphthylacetic acid, indoleacetic acid, lanthanum carbonate and brassin according to the mass ratio of 500:1.25:1.5:1: 2;
s6, selecting 1-2 nodes of stem segments with buds at the top of the cultured bud prepared in the step S4, cutting the stem segments with buds into the second culture medium prepared in the step S5, setting the culture temperature to be 25 ℃, the relative humidity to be 50%, the illumination intensity to be 1800lux, the illumination time per day to be 12h, and culturing for 14 days to prepare a propagation bud;
s7, transplanting the propagation bud prepared in the step S6 into cutting soil located outdoors, and watering and cultivating.
Example 10
A method for cultivating and maintaining saline-alkali tolerant plants, namely suaeda salsa in south comprises the following steps:
s1, airing 500g of southern suaeda glauca seeds for 54 h;
s2, placing the southern suaeda glauca seeds aired in the step S1 under an ultraviolet lamp to irradiate for 2.5 hours;
s3, soaking the southern suaeda glauca seeds aired in the step S2 in water at 25 ℃ for 3 hours to prepare primary treated seeds;
s4, taking 1kg of the culture solution prepared in the preparation example 1 of the culture solution, putting the primary treated seed obtained in the step S3 into the culture solution for immersion culture, and irradiating for 5 days under the conditions that the culture temperature is 25 ℃ and the illumination intensity is 2000lux, wherein the illumination time per day is 12h, so as to prepare a culture bud;
s5, taking 2kg of culture medium, adding naphthylacetic acid and indoleacetic acid into 2kg of culture medium, wherein the mass ratio of the culture medium to the naphthylacetic acid to the indoleacetic acid is 500:1.25:1.5, and uniformly mixing to prepare a first culture medium; adding lanthanum carbonate and brassin into the first culture medium, and uniformly mixing to prepare a second culture medium, wherein the second culture medium is prepared from the culture medium, naphthylacetic acid, indoleacetic acid, lanthanum carbonate and brassin according to the mass ratio of 500:1.25:1.5:1: 2;
s6, selecting 1-2 nodes of stem segments with buds at the top of the cultured bud prepared in the step S4, cutting the stem segments with buds into the second culture medium prepared in the step S5, setting the culture temperature to be 25 ℃, the relative humidity to be 50%, the illumination intensity to be 1800lux, the illumination time per day to be 12h, and culturing for 14 days to prepare a propagation bud;
s7, wiping the breeding buds prepared in the step S6 by dipping the cotton balls in a sodium hypochlorite solution for 10S, and then washing the cotton balls clean with water to obtain pre-sterilized buds; dipping a cotton ball in alcohol solution to wipe the pre-sterilized buds for 5s, then washing the cotton ball with water, and sucking water to obtain the re-sterilized buds;
s8, transplanting the multiple sterilized buds obtained in the step S7 into cutting soil located outdoors, and watering and cultivating.
Example 11
The method for cultivating and maintaining the saline-alkali tolerant plant, suaeda salsa, in this example is different from example 10 in that the culture solution used in step S4 is prepared from culture solution preparation example 2, and the rest is the same as in example 10.
Example 12
The method for cultivating and maintaining the saline-alkali tolerant plant, suaeda salsa, in this example is different from example 10 in that the culture solution used in step S4 is prepared from culture solution preparation example 3, and the rest is the same as in example 10.
Example 13
The method for cultivating and maintaining the saline-alkali tolerant plant, suaeda salsa, in this example is different from example 10 in that the culture solution used in step S4 is prepared from culture solution preparation example 4, and the rest is the same as in example 10.
Example 14
The method for cultivating and maintaining the saline-alkali tolerant plant, suaeda salsa, in this example is different from example 10 in that the culture solution used in step S4 is prepared from culture solution preparation example 5, and the rest is the same as in example 10.
Example 15
The method for cultivating and maintaining the saline-alkali tolerant plant suaeda salsa southern is different from that in the embodiment 10 in that before the multiple sterilized buds are transplanted into the cutting soil in the step S8, the cutting soil is added with the turfy soil and the vermiculite and is uniformly mixed, the mass ratio of the cutting soil to the turfy soil to the vermiculite is 10:1:1, and the rest is the same as that in the embodiment 10.
Comparative example
Comparative example 1
This comparative example is different from example 1 in that gibberellic acid is not contained in the culture solution used in step S1, and the rest is the same as in example 1.
Comparative example 2
This comparative example is different from example 1 in that the culture solution used in step S1 contains no chitosan, and is otherwise the same as that used in example 1.
Comparative example 3
This comparative example is different from example 1 in that benzylaminopurine is not contained in the culture solution used in step S1, and the others are the same as those in example 1.
Performance test
1. Taking southern common seepweed herb seeds, taking 500 common seepweed herb seeds as a group, and performing group culture;
2. a group of southern suaeda glauca seeds were each grown as in examples 1-15;
3. respectively cultivating a group of southern suaeda glauca seeds by adopting the method of the comparative examples 1-3 to serve as the comparative examples 1-3;
TABLE 1 test results of germination rates of Suaeda salsa seeds in test examples 1-15 and comparative examples 1-3
| Test examples | Percentage of germination (%) |
| Test example 1 | 60 |
| Test example 2 | 67 |
| Test example 3 | 68 |
| Test example 4 | 73 |
| Test example 5 | 75 |
| Test example 6 | 77 |
| Test example 7 | 81 |
| Test example 8 | 83 |
| Test example 9 | 84 |
| Test example 10 | 85 |
| Test example 11 | 88 |
| Test example 12 | 90 |
| Test example 13 | 91 |
| Test example 14 | 93 |
| Test example 15 | 95 |
| Comparative example 1 | 51 |
| Comparative example 2 | 55 |
| Comparative example 3 | 52 |
The combination of the test examples 4-6 and the table 1 shows that the root growth and development of the southern suaeda salsa seeds can be promoted by adding the lanthanum carbonate and the brassin, so that the germination survival rate of the southern suaeda salsa seeds is improved; when the culture medium, the naphthylacetic acid, the indoleacetic acid, the lanthanum carbonate and the brassin are used for culturing the suaeda salsa seeds according to the mass ratio of 500:1.25:1.5:1:2, the effect of improving the germination survival rate of the suaeda salsa seeds is most obvious.
It can be seen from the combination of test example 1, comparative examples 1 to 3, and table 1 that the germination survival rate of southern suaeda glauca is improved by the synergistic effect of gibberellic acid, chitosan and benzylaminopurine.
As can be seen from the combination of test examples 9-10 and table 1, by sterilizing the propagation buds inserted into the cutting soil with sodium hypochlorite solution and alcohol solution, viruses and bacteria on the surfaces of the propagation buds can be effectively killed, so that the southern suaeda glauca is not easily damaged by the viruses and bacteria in the subsequent growth process, and the germination survival rate of the southern suaeda glauca is improved.
The present embodiment is only for explaining the present application, and it is not limited to the present application, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present application.
Claims (8)
1. A method for cultivating and maintaining saline-alkali tolerant plants, namely suaeda salsa in south, is characterized by comprising the following steps: the method comprises the following steps:
s1, placing the suaeda salsa seeds into the culture solution to be cultured for 5-6 days to prepare cultured buds; each kilogram of the culture solution is prepared from the following raw materials by weight: gibberellic acid 50-100mg, chitosan 60-120mg, benayl aminopurine 5-10mg, trace element mixture 8-15mg and the balance of water; the trace element mixture comprises calcium nitrate, potassium nitrate and sodium molybdate, and the mass ratio of the calcium nitrate to the potassium nitrate to the sodium molybdate is 1 (1-2) to 1-1.5;
s2, selecting a stem section with buds at the top of the culture bud prepared in the step S1, and cutting the stem section with buds into a culture medium for cultivation to obtain a propagation bud;
and S3, transplanting the propagation bud obtained in the step S2 into cutting soil located outdoors, and watering and cultivating.
2. The method for cultivating and maintaining the saline-alkali tolerant plant, namely the suaeda salsa, as claimed in claim 1, wherein the method comprises the following steps: pre-treating the suaeda glauca seeds in the step S1 before the seeds are placed in a culture solution, wherein the pre-treating comprises the following steps:
1) airing the southern suaeda glauca seeds for 48-72 h;
2) placing the southern suaeda glauca seeds aired in the step 1) under an ultraviolet lamp to irradiate for 2-3 h;
3) and placing the southern suaeda glauca seeds irradiated by the ultraviolet lamp in the step 2) in water at the temperature of 20-30 ℃ for soaking for 3 hours, and taking out for later use.
3. The method for cultivating and maintaining the saline-alkali tolerant plant, namely the suaeda salsa, as claimed in claim 1, wherein the method comprises the following steps: adding naphthylacetic acid and indoleacetic acid into the culture medium before cutting the culture buds in the step S2 into the culture medium, wherein the mass ratio of the culture medium, the naphthylacetic acid and the indoleacetic acid is 500 (1-1.5) to (1-2).
4. The method for cultivating and maintaining the saline-alkali tolerant plant, namely the suaeda salsa, as claimed in claim 3, wherein the method comprises the following steps: after naphthylacetic acid and indoleacetic acid are added into the culture medium, lanthanum carbonate and brassin are added into the culture medium, and the mass ratio of the culture medium to the lanthanum carbonate to the brassin is 500:1 (2-3).
5. The method for cultivating and maintaining the saline-alkali tolerant plant, namely the suaeda salsa, as claimed in claim 1, wherein the method comprises the following steps: the propagation bud is sterilized before being transplanted to cutting soil, and the sterilization treatment comprises the following steps:
s11, wiping the propagation buds with a sodium hypochlorite solution, and then washing the propagation buds with water to obtain pre-sterilized buds;
s12, wiping the pre-sterilized bud obtained in the step S11 with alcohol solution, then washing with water, and sucking water for later use.
6. The method for cultivating and maintaining the saline-alkali tolerant plant, namely the suaeda salsa, as claimed in claim 1, wherein the method comprises the following steps: before the propagation buds are transplanted into the cutting soil in the step S3, turfy soil and vermiculite are added into the cutting soil and are uniformly turned and stirred, and the mass ratio of the cutting soil to the turfy soil to the vermiculite is 10:1: 1.
7. The saline-alkali tolerant plant suaeda salsa south culture solution is characterized in that: each kilogram of the culture solution is prepared from the following raw materials by weight: gibberellic acid 50-100mg, chitosan 60-120mg, benayl aminopurine 5-10mg, trace element mixture 8-15mg and the balance of water; the trace element mixture comprises calcium nitrate, potassium nitrate and sodium molybdate, and the mass ratio of the calcium nitrate to the potassium nitrate to the sodium molybdate is 1 (1-2) to 1-1.5.
8. The saline-alkali tolerant plant suaeda salsa south culture solution of claim 7, wherein: each kilogram of the culture solution is prepared from the following raw materials by weight: 65-90mg of gibberellic acid, 70-100mg of chitosan, 6-9mg of benzylaminopurine, 10-12mg of trace element mixture and the balance of water; the trace element mixture comprises calcium nitrate, potassium nitrate and sodium molybdate, and the mass ratio of the calcium nitrate to the potassium nitrate to the sodium molybdate is 1 (1-2) to 1-1.5.
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