CN101828509B - Water culture technique for anoectochilus formosanus - Google Patents
Water culture technique for anoectochilus formosanus Download PDFInfo
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- CN101828509B CN101828509B CN2010101722219A CN201010172221A CN101828509B CN 101828509 B CN101828509 B CN 101828509B CN 2010101722219 A CN2010101722219 A CN 2010101722219A CN 201010172221 A CN201010172221 A CN 201010172221A CN 101828509 B CN101828509 B CN 101828509B
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- roxburgh anoectochilus
- anoectochilus terminal
- terminal bud
- culture
- nutrient solution
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- Y02P60/216—
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Abstract
The invention provides a water culture technique for anoectochilus formosanus and relates to a pollution-free water (fog) culture production technique for Chinese medicament anoectochilus formosanus. The water culture technique adopts plastic disks and water grooves as culture containers and comprises the following steps of: transplanting the seedlings which are prepared by tissue culture and subjected to cleaning and sterilizing into the plastic disks; wherein the plastic disks are placed in the water grooves with nutrient solution; and performing culture management by fertilizing, changing the nutrient solution, atomizing, lighting, controlling humidity, controlling temperature and changing air. The water culture technique has the advantages that: the survival rate of transplanting the noectochilus formosanus seedlings prepared by tissue culture can be improved to over 98 percent; the fresh weight of the finished products in each square meter area is over 800 g; the production process is simple; the yield is high; the occupied area is small; and the water culture technique is suitable for culture by intensive and pollution-free facilities or scattered culture by farmers.
Description
Technical field
The present invention relates to a kind of nuisanceless water (mist) training production technology of traditional Chinese medicine roxburgh anoectochilus terminal bud.
Background technology
Relevant roxburgh anoectochilus terminal bud artificial cultivation report like " the some culture technique researchs of roxburgh anoectochilus terminal bud " of " subtropical plant communication " 23 (1) the 46-51 pages of reports in 1994, is matrix, plane cultivation with soil, every square metre of bright article 0.36kg of results." Fujian agriculture science and technology " 1998 (5) the 41st pages, report " the indoors artificial culture technique of roxburgh anoectochilus terminal bud ", indoors artificial cultivation roxburgh anoectochilus terminal bud adopts casting bed plantation, every square metre of field planting 200 strains.The tissue cultivating seedling of roxburgh anoectochilus terminal bud generally adopts seedlings (the heavy 1.34g of average strain) to transplant at present, and survival rate reaches more than 92%, and little transplantation of seedlings is difficult, and survival rate difference great disparity is bigger; The bright article 250～300kg of the general per mu yield of artificial cultivation roxburgh anoectochilus terminal bud.
Summary of the invention
The invention reside in provides a kind of water culture technique for anoectochilus formosanus, to improve the tissue cultivating seedling transplanting survival rate of roxburgh anoectochilus terminal bud.
Water culture technique for anoectochilus formosanus of the present invention, adopting vinyl disc and tank is cultivating container, utilizes the roxburgh anoectochilus terminal bud seedling of tissue culture, through cleaning, sterilizing, is transplanted in the vinyl disc, said vinyl disc places in the tank that nutrient solution is housed; Through fertilising, change that nutrient solution, spraying, illumination, control are wet, the planting type of temperature control, ventilation manages.
Whole process of cultivation comprises:
1. seedling production
1) the roxburgh anoectochilus terminal bud aseptic seedling is cultivated in detoxification;
2) breeding roxburgh anoectochilus terminal bud bud;
3) cultivate the roxburgh anoectochilus terminal bud seedling.
2. kind transplantation of seedlings
1) remove the medium of tissue cultivating seedling root with flushing with clean water after, soaked H liquid (i.e. 0.1% carbendazim solution) 8-15 minute;
The roxburgh anoectochilus terminal bud tissue cultivating seedling that 2) will soak H liquid is put into vinyl disc, and vinyl disc is put in the tank;
3) add nutrient solution in the tank.
3. nutrient solution preparation (10L)
(1) macroelement: nitrate of lime [Ca (NO
3)
2-4H
20] 708mg, potassium nitrate (KNO
3) 303mg, ammonium dihydrogen phosphate (ADP) (NH
4H
2PO
4) 115mg, magnesium sulfate (MgSO
4-7H
20) 370mg;
(2) trace element: EDTA disodium iron (Na
2FeEDTA) 7mg, manganese sulphate (MnSO
4-4H
20) 2 mg, boric acid (H
3BO
3) 2 mg, copper sulphate (MgSO
4-5H
20) 0.2 mg, zinc sulphate (MgSO
4-7H
20) 0.4 mg, molybdic acid (H
2MoO
4-4H
20) 0.2 mg;
(3) growth hormone: NAA 40ppm.
1. cultivation management
1) regularly sprays leaf fertilizer: every at a distance from foliage fertilizer of spray in 5-7 days; I.e. 0.1% urea+0.05% potassium dihydrogen phosphate;
2) regularly change nutrient solution: whenever changed nutrient solution once at a distance from 10～15 days,
3) regularly in the tank liquid revolve circulation moving with the water smoke humidification: every in 2 hours tanks liquid revolve circulation moving with water smoke humidification 1 hour, liquid revolved the moving and water smoke humidification of circulation totally 8 hours in one day tank.
4) intensity of illumination: 800-1500lx
5) relative air humidity: 85-95%;
6) temperature: 23-25 ℃;
7) oxygen:>20%; Take a breath 2-3 every day, each 0.5-1 hour.
Remarkable advantage of the present invention:
The present invention adopts the roxburgh anoectochilus terminal bud tissue cultivating seedling survival rate of above-mentioned cultivation method plantation up to more than 98%; More than every square metre of area finished product fresh weight 800g, production technology of the present invention is easy, and output is high; Land used is few, and the scattered cultivation of nuisanceless facility cultivation or peasant household is adapted to intensify.
Embodiment
Embodiment 1
The cultivating chamber that illumination and ventilation condition were wet, controlled in, control temperature automatically controlled to have is an example; In cultivating chamber, set up the cultivation tank regularly; Its arranging density, trend are advisable with the needs of accords with production operation, carry out seedling production, kind transplantation of seedlings, nutrient solution preparation, water planting management by following 1,2,3,4 step requirements then.
1. seedling production
1) the roxburgh anoectochilus terminal bud aseptic seedling is cultivated in detoxification
Selecting the wild roxburgh anoectochilus terminal bud or the tame roxburgh anoectochilus terminal bud plant of robust growth is seedling, and the sterile-processed MS of being implanted in cultivates aseptic seedling in cultivating, and cuts the sprouting top and is forwarded in the bud propagating culture medium in 30-40 days.
2) breeding roxburgh anoectochilus terminal bud bud
Roxburgh anoectochilus terminal bud bud propagating culture medium is MS+6-BA1-4g+NAA0.1-0.5g+ vitamin h 50-100ppm, receives the sprouting top in the bud propagating culture medium and cultivates, and 40-50 days subcultures once.
3) cultivate the roxburgh anoectochilus terminal bud seedling
Roxburgh anoectochilus terminal bud kind bacterium culture medium is 1/2MS+6-BA0.1g+NAA0.5-1.0g+ vitamin h 50-100ppm, moves on to the bud seedling that grows 2-3 blade in kind of the bacterium culture medium, cultivates the cultivation of can emerging in 50-60 days.
2. kind transplantation of seedlings
1) remove the medium of tissue cultivating seedling root with flushing with clean water after, soaked H liquid (i.e. 0.1% carbendazim solution) 10 minutes;
The roxburgh anoectochilus terminal bud tissue cultivating seedling that 2) will soak H liquid is put into vinyl disc, and vinyl disc is put in the tank;
3) add nutrient solution in the tank.
3. nutrient solution preparation (10 liters)
(1) macroelement: nitrate of lime [Ca (NO
3)
2-4H
20] 708mg, potassium nitrate (KNO
3) 303mg, ammonium dihydrogen phosphate (ADP) (NH
4H
2PO
4) 115mg, magnesium sulfate (MgSO
4-7H
20) 370mg;
(2) trace element: EDTA disodium iron (Na
2FeEDTA) 7mg, manganese sulphate (MnSO
4-4H
20) 2 mg, boric acid (H
3BO
3) 2 mg, copper sulphate (MgSO
4-5H
20) 0.2 mg, zinc sulphate (MgSO
4-7H
20) 0.4 mg, molybdic acid (H
2MoO
4-4H
20) 0.2 mg;
(3) growth hormone: NAA 40ppm.
4. cultivation management
1) regularly sprays leaf fertilizer: every at a distance from foliage fertilizer of spray in 5-7 days; I.e. 0.1% urea+0.05% potassium dihydrogen phosphate;
2) regularly change nutrient solution: whenever changed nutrient solution once at a distance from 10～15 days,
3) regularly in the tank liquid revolve circulation moving with the water smoke humidification: every in 2 hours tanks liquid revolve circulation moving with water smoke humidification 1 hour, liquid revolved the moving and water smoke humidification of circulation totally 8 hours in one day tank.
4) intensity of illumination: 800-1500lx
5) relative air humidity: 85-95%;
6) temperature: 23-25 ℃;
7) oxygen:>20%; Take a breath 2-3 every day, each 0.5-1 hour.
Apply fertilizer by above-mentioned cultivation management technology, change nutrient solution, regularly water revolves the moving and spraying of circulation, regularly exhaust, and environmental conditions such as control light, temperature, wet, gas.
Embodiment 2
To have the simple and easy shading screen canopy cultivation that prevents the animal infringement is example; In cultivation net canopy, set up multilayer cultivation tank regularly; Its arranging density, trend are advisable with the needs of accords with production operation; Utilize natural daylight and shading screen control two sun eight cloudy environment, ventilate through noting cooling in manual work spraying or mechanical humidification control air relative moisture, summer; Get final product by embodiment 1 said 1,2,3,4 step requirements carrying out seedling production, transplanting, nutrient solution preparation, management then.
Claims (4)
1. roxburgh anoectochilus terminal bud ciltivating process is characterized in that: adopting vinyl disc and tank is cultivating container, utilizes the roxburgh anoectochilus terminal bud seedling of tissue culture, through cleaning, sterilizing, is transplanted in the vinyl disc, and said vinyl disc places in the tank that nutrient solution is housed; Through fertilising, change that nutrient solution, spraying, illumination, control are wet, temperature control, ventilation carry out cultivation management;
The preparation of said nutrient solution is following:
(1) macroelement: Ca (NO
3)
2-4H
20 708mg, KNO
3303mg, NH
4H
2PO
4115mg, MgSO
4-7H
20 370mg;
(2) trace element: Na
2FeEDTA 7mg, MnSO
4-4H
202 mg, H
3BO
32 mg, MgSO
4-5H
20 0.2 mg, MgSO
4-7H
20 0.4 mg, H
2MoO
4-4H
20 0.2 mg;
(3) growth hormone: NAA 40ppm;
Be mixed with the nutrient solution of 10L.
2. roxburgh anoectochilus terminal bud ciltivating process according to claim 1 is characterized in that: the tissue culture procedures of said roxburgh anoectochilus terminal bud seedling is following:
(1) the roxburgh anoectochilus terminal bud aseptic seedling is cultivated in detoxification;
(2) breeding roxburgh anoectochilus terminal bud bud;
(3) cultivate the roxburgh anoectochilus terminal bud seedling.
3. roxburgh anoectochilus terminal bud ciltivating process according to claim 1 is characterized in that: said sterilization is that the roxburgh anoectochilus terminal bud seedling is soaked in mass concentration is in 0.1% the carbendazim solution 8～15 minutes.
4. roxburgh anoectochilus terminal bud ciltivating process according to claim 1 is characterized in that: said cultivation management is specific as follows:
(1) regularly sprays leaf fertilizer: every at a distance from foliage fertilizer of spray in 5-7 days; Said foliage fertilizer is 0.1% urea+0.05% potassium dihydrogen phosphate;
(2) periodic replacement nutrient solution: whenever changed nutrient solution once at a distance from 10～15 days,
(3) regularly in the tank liquid revolve circulation moving with the water smoke humidification: every in 2 hours tanks liquid revolve circulation moving with water smoke humidification 1 hour, liquid revolved the moving and water smoke humidification of circulation totally 8 hours in one day tank;
(4) intensity of illumination: 800-1500lx
(5) relative air humidity: 85-95%;
(6) temperature: 23-25 ℃;
(7) oxygen:>20%; Take a breath 2-3 every day, each 0.5-1 hour.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101722219A CN101828509B (en) | 2010-05-14 | 2010-05-14 | Water culture technique for anoectochilus formosanus |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101722219A CN101828509B (en) | 2010-05-14 | 2010-05-14 | Water culture technique for anoectochilus formosanus |
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| Publication Number | Publication Date |
|---|---|
| CN101828509A CN101828509A (en) | 2010-09-15 |
| CN101828509B true CN101828509B (en) | 2012-02-29 |
Family
ID=42712852
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|---|---|---|---|
| CN2010101722219A Expired - Fee Related CN101828509B (en) | 2010-05-14 | 2010-05-14 | Water culture technique for anoectochilus formosanus |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105409740A (en) * | 2015-11-11 | 2016-03-23 | 福建省亚热带植物研究所 | Water cultivation method of anoectochilus roxburghii |
| CN108633635A (en) * | 2018-04-26 | 2018-10-12 | 南靖县葛园农林科技有限公司 | A kind of roxburgh anoectochilus terminal bud high-efficiency and energy-saving type production technology and its production equipment |
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| CN102090341B (en) * | 2010-12-24 | 2012-02-01 | 福建农林大学 | Method for rapidly breeding jewel orchid |
| CN102100171B (en) * | 2010-12-30 | 2012-02-22 | 福建永安天奇健金线莲生态实业有限公司 | Aqueous culture method of anoectochilus |
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| CN101213942B (en) * | 2008-01-10 | 2012-03-07 | 浙江省中药研究所有限公司 | Medicinal anoectochilus Formosan tissue culture one-step seedling establishment fast propogation method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105409740A (en) * | 2015-11-11 | 2016-03-23 | 福建省亚热带植物研究所 | Water cultivation method of anoectochilus roxburghii |
| CN108633635A (en) * | 2018-04-26 | 2018-10-12 | 南靖县葛园农林科技有限公司 | A kind of roxburgh anoectochilus terminal bud high-efficiency and energy-saving type production technology and its production equipment |
| CN108633635B (en) * | 2018-04-26 | 2019-09-20 | 南靖县葛园农林科技有限公司 | A kind of roxburgh anoectochilus terminal bud high-efficiency and energy-saving type production technology and its production equipment |
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| CN101828509A (en) | 2010-09-15 |
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