CN101828509A - Water culture technique for anoectochilus formosanus - Google Patents

Water culture technique for anoectochilus formosanus Download PDF

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Publication number
CN101828509A
CN101828509A CN201010172221A CN201010172221A CN101828509A CN 101828509 A CN101828509 A CN 101828509A CN 201010172221 A CN201010172221 A CN 201010172221A CN 201010172221 A CN201010172221 A CN 201010172221A CN 101828509 A CN101828509 A CN 101828509A
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water
culture
nutrient solution
terminal bud
culture technique
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CN101828509B (en
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周以飞
张绪璋
潘大仁
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Fujian Agriculture and Forestry University
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Fujian Agriculture and Forestry University
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    • Y02P60/216—

Abstract

The invention provides a water culture technique for anoectochilus formosanus and relates to a pollution-free water (fog) culture production technique for Chinese medicament anoectochilus formosanus. The water culture technique adopts plastic disks and water grooves as culture containers and comprises the following steps of: transplanting the seedlings which are prepared by tissue culture and subjected to cleaning and sterilizing into the plastic disks; wherein the plastic disks are placed in the water grooves with nutrient solution; and performing culture management by fertilizing, changing the nutrient solution, atomizing, lighting, controlling humidity, controlling temperature and changing air. The water culture technique has the advantages that: the survival rate of transplanting the noectochilus formosanus seedlings prepared by tissue culture can be improved to over 98 percent; the fresh weight of the finished products in each square meter area is over 800 g; the production process is simple; the yield is high; the occupied area is small; and the water culture technique is suitable for culture by intensive and pollution-free facilities or scattered culture by farmers.

Description

A kind of water culture technique for anoectochilus formosanus
Technical field
The present invention relates to a kind of nuisanceless water (mist) training production technology of traditional Chinese medicine roxburgh anoectochilus terminal bud.
Background technology
Relevant roxburgh anoectochilus terminal bud artificial cultivation report is as " subtropical plant communication " 23(1 in 1994) " the some culture technique researchs of roxburgh anoectochilus terminal bud " of the 46-51 page of report, be matrix, plane cultivation with soil, every square metre of results aquatic foods product 0.36kg." Fujian agriculture science and technology " 1998 (5) the 41st pages, report " the indoors artificial culture technique of roxburgh anoectochilus terminal bud ", indoors artificial cultivation roxburgh anoectochilus terminal bud adopts casting bed plantation, every square metre of field planting 200 strains.The tissue cultivating seedling of roxburgh anoectochilus terminal bud generally adopts seedlings (the heavy 1.34g of average strain) to transplant at present, and survival rate reaches more than 92%, and little transplantation of seedlings is difficult, and survival rate difference great disparity is bigger; The bright product 250～300kg of the general per mu yield of artificial cultivation roxburgh anoectochilus terminal bud.
Summary of the invention
The invention reside in provides a kind of water culture technique for anoectochilus formosanus, to improve the tissue cultivating seedling transplanting survival rate of roxburgh anoectochilus terminal bud.
Water culture technique for anoectochilus formosanus of the present invention, adopting vinyl disc and tank is cultivating container, utilizes the roxburgh anoectochilus terminal bud seedling of tissue culture, through cleaning, sterilizing, is transplanted in the vinyl disc, described vinyl disc places in the tank that nutrient solution is housed; Through fertilising, change that nutrient solution, spraying, illumination, control are wet, the planting type of temperature control, ventilation manages.
Whole process of cultivation comprises:
1. seedling production
1) the roxburgh anoectochilus terminal bud aseptic seedling is cultivated in detoxification;
2) breeding roxburgh anoectochilus terminal bud bud;
3) cultivate the roxburgh anoectochilus terminal bud seedling.
2. kind transplantation of seedlings
1) remove the medium of tissue cultivating seedling root with flushing with clean water after, soaked H liquid (i.e. 0.1% carbendazim solution) 8-15 minute;
2) the roxburgh anoectochilus terminal bud tissue cultivating seedling that will soak H liquid is put into vinyl disc, and vinyl disc is put in the tank;
3) add nutrient solution in the tank.
3. nutrient solution preparation (10L)
(1) macroelement: nitrate of lime [Ca (NO 3) 2-4H 20] 708mg, potassium nitrate (KNO 3) 303mg, ammonium dihydrogen phosphate (ADP) (NH 4H 2PO 4) 115mg, magnesium sulfate (MgSO 4-7H 20) 370mg;
(2) trace element: EDTA disodium iron (Na 2FeEDTA) 7mg, manganese sulphate (MnSO 4-4H 20) 2mg, boric acid (H 3BO 3) 2mg, copper sulphate (MgSO 4-5H 20) 0.2mg, zinc sulphate (MgSO 4-7H 20) 0.4mg, molybdic acid (H 2MoO 4-4H 20) 0.2mg;
(3) growth hormone: NAA40ppm.
4. cultivation management
1) regularly sprays leaf fertilizer: every foliage fertilizer of spray in 5-7 days; I.e. 0.1% urea+0.05% potassium dihydrogen phosphate;
2) regularly change nutrient solution: changed nutrient solution once every 10～15 days;
3) regularly in the tank liquid revolve the moving and water smoke humidification of circulation: liquid revolved the moving and water smoke humidification of circulation 1 hour in 2 hours tanks, and liquid revolved the moving and water smoke humidification of circulation totally 8 hours in one day tank;
4) intensity of illumination: 800-1500lx;
5) relative air humidity: 85-95%;
6) temperature: 23-25 ℃;
7) oxygen:〉20%; Take a breath 2-3 every day, each 0.5-1 hour.
Remarkable advantage of the present invention:
The present invention adopts the roxburgh anoectochilus terminal bud tissue cultivating seedling survival rate of above-mentioned cultivation method plantation up to more than 98%, more than every square metre of area finished product fresh weight 800g, production technology of the present invention is easy, the output height, land used is few, and the scattered cultivation of nuisanceless facility cultivation or peasant household is adapted to intensify.
Embodiment
Embodiment 1
The cultivating chamber that illumination and ventilation condition were wet, controlled in, control temperature automatically controlled to have is an example, in cultivating chamber, set up the cultivation tank regularly, its arranging density, trend are advisable with the needs that meet production operation, carry out seedling production, kind transplantation of seedlings, nutrient solution preparation, water planting management by following 1,2,3,4 step requirements then.
1. seedling production
1) the roxburgh anoectochilus terminal bud aseptic seedling is cultivated in detoxification
Selecting the wild roxburgh anoectochilus terminal bud or the tame roxburgh anoectochilus terminal bud plant of robust growth is seedling, and the sterile-processed MS of being implanted in cultivates aseptic seedling in cultivating, and cuts the sprouting top and is forwarded in the bud propagating culture medium in 30-40 days.
2) breeding roxburgh anoectochilus terminal bud bud
Roxburgh anoectochilus terminal bud bud propagating culture medium is MS+6-BA1-4g+NAA0.1-0.5g+ vitamin h 50-100ppm, the sprouting top is received in the bud propagating culture medium cultivate, and 40-50 days subcultures once.
3) cultivate the roxburgh anoectochilus terminal bud seedling
Roxburgh anoectochilus terminal bud kind bacterium culture medium is 1/2MS+6-BA0.1g+NAA0.5-1.0g+ vitamin h 50-100ppm, and the bud seedling that grows 2-3 blade is moved on in kind of the bacterium culture medium, cultivates the cultivation of can emerging in 50-60 days.
2. kind transplantation of seedlings
1) remove the medium of tissue cultivating seedling root with flushing with clean water after, soaked H liquid (i.e. 0.1% carbendazim solution) 10 minutes;
2) the roxburgh anoectochilus terminal bud tissue cultivating seedling that will soak H liquid is put into vinyl disc, and vinyl disc is put in the tank;
3) add nutrient solution in the tank.
3. nutrient solution preparation (10 liters)
(1) macroelement: nitrate of lime [Ca (NO 3) 2-4H 20] 708mg, potassium nitrate (KNO 3) 303mg, ammonium dihydrogen phosphate (ADP) (NH 4H 2PO 4) 115mg, magnesium sulfate (MgSO 4-7H 20) 370mg;
(2) trace element: EDTA disodium iron (Na 2FeEDTA) 7mg, manganese sulphate (MnSO 4-4H 20) 2mg, boric acid (H 3BO 3) 2mg, copper sulphate (MgSO 4-5H 20) 0.2mg, zinc sulphate (MgSO 4-7H 20) 0.4mg, molybdic acid (H 2MoO 4-4H 20) 0.2mg;
(3) growth hormone: NAA40ppm.
4. cultivation management
1) regularly sprays leaf fertilizer: every foliage fertilizer of spray in 5-7 days; I.e. 0.1% urea+0.05% potassium dihydrogen phosphate;
2) regularly change nutrient solution: changed nutrient solution once every 10～15 days;
3) regularly in the tank liquid revolve the moving and water smoke humidification of circulation: liquid revolved the moving and water smoke humidification of circulation 1 hour in 2 hours tanks, and liquid revolved the moving and water smoke humidification of circulation totally 8 hours in one day tank;
4) intensity of illumination: 800-1500lx;
5) relative air humidity: 85-95%;
6) temperature: 23-25 ℃;
7) oxygen:〉20%; Take a breath 2-3 every day, each 0.5-1 hour.
Apply fertilizer by above-mentioned cultivation management technology, change nutrient solution, regularly water revolves the moving and spraying of circulation, regularly exhaust, and environmental conditions such as control light, temperature, wet, gas.
Embodiment 2
To have the simple and easy shading screen canopy cultivation that prevents the animal infringement is example, in cultivation net canopy, set up multilayer cultivation tank regularly, its arranging density, trend are advisable with the needs that meet production operation, utilize natural daylight and shading screen control two sun eight cloudy environment, ventilate by noting cooling in artificial spraying or mechanical humidification control relative air humidity, summer; Get final product by embodiment 1 described 1,2,3,4 step requirements carrying out seedling production, transplanting, nutrient solution preparation, management then.
 

Claims (5)

1. water culture technique for anoectochilus formosanus is characterized in that: adopting vinyl disc and tank is cultivating container, utilizes the roxburgh anoectochilus terminal bud seedling of tissue culture, through cleaning, sterilization, be transplanted in the vinyl disc, described vinyl disc places in the tank that nutrient solution is housed; Through fertilising, change that nutrient solution, spraying, illumination, control are wet, temperature control, ventilation carry out cultivation management.
2. water culture technique for anoectochilus formosanus according to claim 1 is characterized in that: the tissue culture procedures of described roxburgh anoectochilus terminal bud seedling is as follows:
(1) the roxburgh anoectochilus terminal bud aseptic seedling is cultivated in detoxification;
(2) breeding roxburgh anoectochilus terminal bud bud;
(3) cultivate the roxburgh anoectochilus terminal bud seedling.
3. water culture technique for anoectochilus formosanus according to claim 1 is characterized in that: described sterilization is that the roxburgh anoectochilus terminal bud seedling is soaked in mass concentration is in 0.1% the carbendazim solution 8～15 minutes.
4. water culture technique for anoectochilus formosanus according to claim 1 is characterized in that: being formulated as follows of described nutrient solution:
(1) macroelement: nitrate of lime [Ca (NO 3) 2-4H 20] 708mg, potassium nitrate (KNO 3) 303mg, ammonium dihydrogen phosphate (ADP) (NH 4H 2PO 4) 115mg, magnesium sulfate (MgSO 4-7H 20) 370mg;
(2) trace element: EDTA disodium iron (Na 2FeEDTA) 7mg, manganese sulphate (MnSO 4-4H 20) 2mg, boric acid (H 3BO 3) 2mg, copper sulphate (MgSO 4-5H 20) 0.2mg, zinc sulphate (MgSO 4-7H 20) 0.4mg, molybdic acid (H 2MoO 4-4H 20) 0.2mg;
(3) growth hormone: NAA40ppm;
Be mixed with the nutrient solution of 10L.
5. water culture technique for anoectochilus formosanus according to claim 1 is characterized in that: described cultivation management is specific as follows:
1) regularly sprays leaf fertilizer: every foliage fertilizer of spray in 5-7 days; Described foliage fertilizer is 0.1% urea+0.05% potassium dihydrogen phosphate;
2) periodic replacement nutrient solution: changed nutrient solution once every 10～15 days;
3) regularly in the tank liquid revolve the moving and water smoke humidification of circulation: liquid revolved the moving and water smoke humidification of circulation 1 hour in 2 hours tanks, and liquid revolved the moving and water smoke humidification of circulation totally 8 hours in one day tank;
4) intensity of illumination: 800-1500lx;
5) relative air humidity: 85-95%;
6) temperature: 23-25 ℃;
7) oxygen:〉20%; Take a breath 2-3 every day, each 0.5-1 hour.
CN2010101722219A 2010-05-14 2010-05-14 Water culture technique for anoectochilus formosanus Expired - Fee Related CN101828509B (en)

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* Cited by examiner, † Cited by third party
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CN102090341A (en) * 2010-12-24 2011-06-15 福建农林大学 Method for rapidly breeding jewel orchid
CN102090303A (en) * 2010-11-10 2011-06-15 天津滨海国际花卉科技园区股份有限公司 Water culture method for drosera capensis
CN102100171A (en) * 2010-12-30 2011-06-22 福建永安天奇健金线莲生态实业有限公司 Aqueous culture method of anoectochilus
CN102511366A (en) * 2011-12-27 2012-06-27 天津滨海国际花卉科技园区股份有限公司 Hydroponic method for hot lip grass
CN102823412A (en) * 2012-09-17 2012-12-19 地缘（厦门）生物科技有限公司 Anoectochilus roburghii pollution-free planting method
CN103004563A (en) * 2012-11-27 2013-04-03 天津滨海国际花卉科技园区股份有限公司 Water culture method for gynura aurantiaca
CN103563618A (en) * 2013-10-12 2014-02-12 杭州市园林绿化股份有限公司 High-efficiency environmental-friendly bare-root transplantation method for plant seedlings
CN104106451A (en) * 2014-06-20 2014-10-22 铜陵梧桐树农业发展有限公司 Soilless vegetable cultivating method
CN105010109A (en) * 2015-06-30 2015-11-04 广西壮族自治区药用植物园 Method for water planting grosvenor momordica
CN105830884A (en) * 2016-03-29 2016-08-10 福建农林大学 Ecological-simulated water-surface cultivation method for Anoectochilus roxburghii
CN106386440A (en) * 2016-09-05 2017-02-15 深圳市双赢伟业科技股份有限公司 Standardized soilless cultivation method and standardized soilless cultivation method for traditional Chinese medicine
CN107787819A (en) * 2017-11-02 2018-03-13 福建农林大学 A kind of orchid roxburgh anoectochilus terminal bud batch production hydroponics technology
CN108633635A (en) * 2018-04-26 2018-10-12 南靖县葛园农林科技有限公司 A kind of roxburgh anoectochilus terminal bud high-efficiency and energy-saving type production technology and its production equipment
CN109206188A (en) * 2018-12-03 2019-01-15 九州天润（武汉）中药研究院有限公司 A kind of mill water culture nutrient solution and its ciltivating process of Herba Epimedii

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CN105409740B (en) * 2015-11-11 2020-01-31 福建省亚热带植物研究所 anoectochilus roxburghii water culture method

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CN1961651A (en) * 2005-11-07 2007-05-16 中国医学科学院药用植物研究所 Application of mycorrhizal fungi in large-scale plantation of gold thread lotus
CN101213942A (en) * 2008-01-10 2008-07-09 浙江省中药研究所有限公司 Medicinal anoectochilus Formosan tissue culture one-step seedling establishment fast replication method

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CN1141866C (en) * 2001-01-19 2004-03-17 大理市大顺绿化有限公司 Nutritive liquid for floating breeding of flower
CN1647610A (en) * 2004-01-19 2005-08-03 福建农林大学 Roxburgh anoectochilus cultivating device and technology
CN1961651A (en) * 2005-11-07 2007-05-16 中国医学科学院药用植物研究所 Application of mycorrhizal fungi in large-scale plantation of gold thread lotus
CN101213942A (en) * 2008-01-10 2008-07-09 浙江省中药研究所有限公司 Medicinal anoectochilus Formosan tissue culture one-step seedling establishment fast replication method

Cited By (20)

* Cited by examiner, † Cited by third party
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CN102090303A (en) * 2010-11-10 2011-06-15 天津滨海国际花卉科技园区股份有限公司 Water culture method for drosera capensis
CN102090303B (en) * 2010-11-10 2013-02-20 天津滨海国际花卉科技园区股份有限公司 Water culture method for drosera capensis
CN102090341B (en) * 2010-12-24 2012-02-01 福建农林大学 Method for rapidly breeding jewel orchid
CN102090341A (en) * 2010-12-24 2011-06-15 福建农林大学 Method for rapidly breeding jewel orchid
CN102100171A (en) * 2010-12-30 2011-06-22 福建永安天奇健金线莲生态实业有限公司 Aqueous culture method of anoectochilus
CN102100171B (en) * 2010-12-30 2012-02-22 福建永安天奇健金线莲生态实业有限公司 Aqueous culture method of anoectochilus
CN102511366A (en) * 2011-12-27 2012-06-27 天津滨海国际花卉科技园区股份有限公司 Hydroponic method for hot lip grass
CN102823412B (en) * 2012-09-17 2015-08-05 地缘（厦门）生物科技有限公司 Roxburgh anoectochilus terminal bud non-polluted planting method
CN102823412A (en) * 2012-09-17 2012-12-19 地缘（厦门）生物科技有限公司 Anoectochilus roburghii pollution-free planting method
CN103004563A (en) * 2012-11-27 2013-04-03 天津滨海国际花卉科技园区股份有限公司 Water culture method for gynura aurantiaca
CN103563618A (en) * 2013-10-12 2014-02-12 杭州市园林绿化股份有限公司 High-efficiency environmental-friendly bare-root transplantation method for plant seedlings
CN104106451A (en) * 2014-06-20 2014-10-22 铜陵梧桐树农业发展有限公司 Soilless vegetable cultivating method
CN105010109A (en) * 2015-06-30 2015-11-04 广西壮族自治区药用植物园 Method for water planting grosvenor momordica
CN105830884A (en) * 2016-03-29 2016-08-10 福建农林大学 Ecological-simulated water-surface cultivation method for Anoectochilus roxburghii
CN105830884B (en) * 2016-03-29 2018-07-27 福建农林大学 A kind of method of Ecology water-surface cultivation roxburgh anoectochilus terminal bud
CN106386440A (en) * 2016-09-05 2017-02-15 深圳市双赢伟业科技股份有限公司 Standardized soilless cultivation method and standardized soilless cultivation method for traditional Chinese medicine
CN107787819A (en) * 2017-11-02 2018-03-13 福建农林大学 A kind of orchid roxburgh anoectochilus terminal bud batch production hydroponics technology
CN108633635A (en) * 2018-04-26 2018-10-12 南靖县葛园农林科技有限公司 A kind of roxburgh anoectochilus terminal bud high-efficiency and energy-saving type production technology and its production equipment
CN108633635B (en) * 2018-04-26 2019-09-20 南靖县葛园农林科技有限公司 A kind of roxburgh anoectochilus terminal bud high-efficiency and energy-saving type production technology and its production equipment
CN109206188A (en) * 2018-12-03 2019-01-15 九州天润（武汉）中药研究院有限公司 A kind of mill water culture nutrient solution and its ciltivating process of Herba Epimedii

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