CN105123479A - Water culture transplanting method for cherry tissue culture seedlings - Google Patents
Water culture transplanting method for cherry tissue culture seedlings Download PDFInfo
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- CN105123479A CN105123479A CN201510562058.XA CN201510562058A CN105123479A CN 105123479 A CN105123479 A CN 105123479A CN 201510562058 A CN201510562058 A CN 201510562058A CN 105123479 A CN105123479 A CN 105123479A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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- Developmental Biology & Embryology (AREA)
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Abstract
Provided is a water culture transplanting method for cherry tissue culture seedlings. Rooting culture bottle seedlings are taken out and trained in sunlight by closing a bottle, a seal is removed, and training is performed again; roots are cleaned, and lower leaves are removed; a nutrient solution is prepared; a plurality of water culture boxes internally provided with planting hole foam plates are sequentially connected through hoses; the cleaned bottle seedlings are planted in planting holes, and the nutrient solution is injected into the water culture boxes so that the nutrient solution in all the water culture boxes can cyclically flow; all the water culture boxes are covered with plastic films so that a closed space can be formed, transition cultivation is performed, then all the films are removed, and ventilation growth is performed; water culture is performed, and the seedlings are pulled out to be transplanted; the single water culture small seedlings are moved into a nutrient bowl filled with substrates, the seedlings are moves into an outdoor shade, the seedlings are moved into field after adapting to the open field growing environment, management is performed according to a field seedling cultivating method, and water culture transplanting of the cherry tissue culture seedlings is completed. The transplanting method can promote leaf epiderm cells to be developed to be mature, conversion from heterotrophic metabolism to autotrophic metabolism can be fast completed, and the survival rate reaches more than 90%.
Description
Technical field
The invention belongs to field of plant tissue culture technique, relate to a kind of cherry group and train seedling water planting transplanting method of taking root, cherry transplanting survival rate can be made to reach more than 90%, and management is simple, production cost is low.
Background technology
In recent years, plant tissue culture technique has entered the production application stage, and be widely used in flowers, the large-scale rapid propagation in vitro of fruit tree, plantlet in vitro forms industrialization in international market.Tissue cultures as the important means of cherry seedling detoxification, and realizes the fast breeding of nursery stock, and replace the growth condition of common seedling more and more extensive with group training detoxic seedling, application prospect is also very wide.
Plant tissue culture detoxifying fast breeding was produced in manual control condition's next year four seasons, and obtained a large amount of group training detoxification nursery stocks at short notice.But the transplanting of plantlet in vitro, arrives outdoor indoor, transformed to natural environmental condition gradually by the low light level in bottle, constant temperature, nearly saturated humidity, gnotobasis, technical difficulty is larger.Due to the blade of differentiation and growth under high humidity, the low light level, heterotrophism condition, leaf cuticle, wax coat are undeveloped, leaf texture gap is large, palisade tissue is thin, and the conducting system of stem grows imperfection in addition, and root is without absorption function or extremely low, the a large amount of dehydration of blade is very easily caused when transplanting high ambient temperature or humidity is lower, and root system can not absorb water supplementary effectively, therefore it is withered very easily to wilt, and causes and transplants unsuccessfully.Especially transplant in common greenhouse by solar heat or plastic tunnel, by the impact of Various Seasonal temp. and humidity, transplanting effect has very large difference.
Summary of the invention
The present invention be directed in prior art and transplant the lower problem of effect and a kind of water planting transplanting method improving the cherry plantlet in vitro of cherry plantlet in vitro transplanting survival rate is provided.
For achieving the above object, the present invention adopts following technical scheme: a kind of water planting transplanting method of cherry plantlet in vitro, specifically carries out according to the following steps:
Seedling is practiced in the indoor of a, seedling of taking root: take out through culture of rootage, the bottle seedling that reaches transplanting standard from culturing room, be placed in greenhouse by solar heat, close bottle and take exercise after 5 ~ 7 days, throw off blake bottle sealing, the running water of injecting height 0.5cm in bottle, then temper 3 ~ 5 days, then carry out water planting;
Index in step a mainly by bottle seedling of taking root from culturing room's growth conditions to the transition of transplanting condition, concrete number of days sums up out from nursery stock transplanting for many years; The running water of injecting height 0.5cm, object is the combination of air-isolation with medium, prevents a bottle seedling pollution, is not evaporated just before the number of amount terminates with hardening.
B, cleaning plantlet in vitro: the bottle seedling tweezers of having taken exercise are taken out gently from bottle, washes the medium being attached to root with clear water off, remove lower blade, reduce the damage of plantlet in vitro root, stem, leaf as far as possible;
Preparation nutrient solution: by mass fraction, water intaking 1000 parts, 0.9 part, potassium nitrate, 0.8 part, ammonium nitrate, potassium dihydrogen phosphate 0.85 part, 0.18 part, magnesium sulfate, 0.22 part, calcium chloride, manganese sulphate 0.11 part, 0.04 part, zinc sulphate and 0.18 part, ferrous sulfate, mix respectively;
The preparation of this nutrient solution calculates by mass fraction.Such as: preparation 100kg nutrient solution, then quality=(summation of 1000 parts/all elements number) × 100kg of water, all the other elements calculate equally.For plant puies forward the nutrient needed for arch normal growth when nutrient solution is soilless culture, different planting types, cultivar need trace different in a large number without element.The composition of this nutrient solution and concentration configure according to the macroelement and concentration of producing upper cherry root media, is mainly and promotes that seedling root system and shoot and leaf growth provide certain nutrient.
C, preparation solution culture facilities: the hydroponic box getting multiple dark 20cm, wide 80 ~ 100cm, long 600 ~ 800cm, be placed in successively in greenhouse, between each hydroponic box with flexible pipe successively, be connected, in each hydroponic box, all place the cystosepiment of thickness 0.3 ~ 1cm, this cystosepiment is processed with multiple hole of planting for fixing seedling;
D, the plantlet in vitro of taking root cleaned planted and plants in hole on cystosepiment, nutrient solution is injected in all hydroponic boxes, make the height of each hydroponic box Middle nutrition liquid be that the height of 10cm(nutrient solution can fluctuate, if seedling root system of taking root is little, then nutrient solution is little below or above 10cm impact, but too little root system growing environment is just little, excessive desired nutritional liquid measure is just large), now, cystosepiment floats on nutrient solution, open water pump, all hydroponic box Middle nutrition liquid recycle streams are moved;
E, covered rearing with plastic film: with the whole hydroponic box of covered rearing with plastic film, form a space comparatively closed, Rooted Cuttings this is comparatively closed, cultivate 3 ~ 5 days by space transition, then with 5 ~ 10 day time all throw off film (can every day by the partial breakaway of plastic film or in film upper shed until all throw off.General purpose constantly adds forced ventilation, strengthens the adaptability that little nursery stock grows in atmosphere gradually), growth of ventilating; When water planting 30 ~ 40 days, main root Gabor transform, hair root increases, blade turn green thickening time, extract and carry out point planting;
F, outdoor point of cultivation management: 1 ︰ 0.3 in mass ratio, fetch earth respectively and decomposed manure, mix, form matrix; By the seedling of water planting in greenhouse, individual plant moves into and is filled with in the nutritive cube of matrix, move in outdoor shade, timely water spray or water, make nursery stock adapt to open country growing environment gradually, when waiting seedling to grow in nutritive cube highly for about 10cm, band soil mud enters land for growing field crops, manage with reference to field seedling raising method, the water planting completing cherry plantlet in vitro is transplanted.
The present invention does not form perfect cuticula according to cherry plantlet in vitro its blade before bottle outlet, the physiological characteristics such as effective photosynthesis can not be carried out, use suspension culture, make plantlet in vitro under comparatively adapt circumstance condition, promote that epidermal leaf cells is reached maturity, complete the transformation from heterotroph metabolism to autotrophic type metabolism quickly, reach the object improving cherry plantlet in vitro transplant survival.This technology is implemented simple, and cost is low, and transplanting survival rate reaches more than 90%.
Embodiment
embodiment 1
Take out through culture of rootage, the bottle seedling that reaches transplanting standard from culturing room, be placed in greenhouse by solar heat, close after bottle tempers 5 days, throw off blake bottle sealing, the running water of injecting height 0.5cm in bottle, then temper 5 days, then carry out water planting; The bottle seedling tweezers of having taken exercise are taken out gently from bottle, washes the medium being attached to root with clear water off, remove lower blade, reduce the damage of plantlet in vitro root, stem, leaf as far as possible; By mass fraction, water intaking 1000 parts, 0.9 part, potassium nitrate, 0.8 part, ammonium nitrate, potassium dihydrogen phosphate 0.85 part, 0.18 part, magnesium sulfate, 0.22 part, calcium chloride, manganese sulphate 0.11 part, 0.04 part, zinc sulphate and 0.18 part, ferrous sulfate respectively, mix, be made into nutrient solution; Get multiple hydroponic box, be placed in greenhouse successively, be connected successively with flexible pipe between each hydroponic box, in each hydroponic box, all place the cystosepiment of thickness 0.3cm, this cystosepiment is processed with multiple hole of planting for fixing seedling; Planted by the plantlet in vitro of taking root cleaned plants in hole on cystosepiment, and injected by nutrient solution in all hydroponic boxes, now, cystosepiment floats on nutrient solution, opens water pump, and all hydroponic box Middle nutrition liquid recycle streams are moved; With the whole hydroponic box of covered rearing with plastic film, form a space comparatively closed, Rooted Cuttings cultivates 3 days in the space transition that this is comparatively closed, more all throws off film with 10 day time, growth of ventilating; When water planting 30 days, main root Gabor transform, hair root increases, blade turn green thickening time, extract and carry out point planting; 1 ︰ 0.3, fetches earth respectively and decomposed manure, mixes in mass ratio, forms matrix; By the seedling of water planting in greenhouse, individual plant moves into and is filled with in the nutritive cube of matrix, move in outdoor shade, timely water spray or water, make nursery stock adapt to open country growing environment gradually, when waiting seedling to grow in nutritive cube highly for about 10cm, band soil mud enters land for growing field crops, manage with reference to field seedling raising method, the water planting completing cherry plantlet in vitro is transplanted.
embodiment 2
Take out through culture of rootage, the bottle seedling that reaches transplanting standard from culturing room, be placed in greenhouse by solar heat, close after bottle tempers 7 days, throw off blake bottle sealing, the running water of injecting height 0.5cm in bottle, then temper 3 days, then carry out water planting; The bottle seedling tweezers of having taken exercise are taken out gently from bottle, washes the medium being attached to root with clear water off, remove lower blade, reduce the damage of plantlet in vitro root, stem, leaf as far as possible; By mass fraction, water intaking 1000 parts, 0.9 part, potassium nitrate, 0.8 part, ammonium nitrate, potassium dihydrogen phosphate 0.85 part, 0.18 part, magnesium sulfate, 0.22 part, calcium chloride, manganese sulphate 0.11 part, 0.04 part, zinc sulphate and 0.18 part, ferrous sulfate respectively, mix, be made into nutrient solution; Get multiple hydroponic box, be placed in greenhouse successively, between each hydroponic box with flexible pipe successively, be connected, in each hydroponic box, all place the cystosepiment of thickness 1cm, this cystosepiment be processed with multiple hole of planting for fixing seedling; The plantlet in vitro of taking root cleaned is planted plants in hole on cystosepiment, and injected by nutrient solution in all hydroponic boxes, now, cystosepiment floats on nutrient solution, opens water pump, and all hydroponic box Middle nutrition liquid recycle streams are moved; With the whole hydroponic box of covered rearing with plastic film, form a space comparatively closed, Rooted Cuttings cultivates 5 days in the space transition that this is comparatively closed, more all throws off film ventilation growth with 5 day time; When water planting 40 days, main root Gabor transform, hair root increases, blade turn green thickening time, extract and carry out point planting; 1 ︰ 0.3, fetches earth respectively and decomposed manure, mixes in mass ratio, forms matrix; By the seedling of water planting in greenhouse, individual plant moves into and is filled with in the nutritive cube of matrix, move in outdoor shade, timely water spray or water, make nursery stock adapt to open country growing environment gradually, when waiting seedling to grow in nutritive cube highly for about 10cm, band soil mud enters land for growing field crops, manage with reference to field seedling raising method, the water planting completing cherry plantlet in vitro is transplanted.
embodiment 3
Take out through culture of rootage, the bottle seedling that reaches transplanting standard from culturing room, be placed in greenhouse by solar heat, close after bottle tempers 6 days, throw off blake bottle sealing, the running water of injecting height 0.5cm in bottle, then temper 4 days, then carry out water planting; The bottle seedling tweezers of having taken exercise are taken out gently from bottle, washes the medium being attached to root with clear water off, remove lower blade, reduce the damage of plantlet in vitro root, stem, leaf as far as possible; By mass fraction, water intaking 1000 parts, 0.9 part, potassium nitrate, 0.8 part, ammonium nitrate, potassium dihydrogen phosphate 0.85 part, 0.18 part, magnesium sulfate, 0.22 part, calcium chloride, manganese sulphate 0.11 part, 0.04 part, zinc sulphate and 0.18 part, ferrous sulfate respectively, mix, be made into nutrient solution; Get multiple hydroponic box, be placed in greenhouse successively, between each hydroponic box with flexible pipe successively, be connected, in each hydroponic box, all place the cystosepiment of thickness 0.7cm, this cystosepiment be processed with multiple hole of planting for fixing seedling; The plantlet in vitro of taking root cleaned is planted plants in hole on cystosepiment, and injected by nutrient solution in all hydroponic boxes, now, cystosepiment floats on nutrient solution, opens water pump, and all hydroponic box Middle nutrition liquid recycle streams are moved; With the whole hydroponic box of covered rearing with plastic film, form a space comparatively closed, Rooted Cuttings cultivates 4 days in the space transition that this is comparatively closed, more all throws off film ventilation growth with 7 day time; When water planting 35 days, main root Gabor transform, hair root increases, blade turn green thickening time, extract and carry out point planting; 1 ︰ 0.3, fetches earth respectively and decomposed manure, mixes in mass ratio, forms matrix; By the seedling of water planting in greenhouse, individual plant moves into and is filled with in the nutritive cube of matrix, move in outdoor shade, timely water spray or water, make nursery stock adapt to open country growing environment gradually, when waiting seedling to grow in nutritive cube highly for about 10cm, band soil mud enters land for growing field crops, manage with reference to field seedling raising method, the water planting completing cherry plantlet in vitro is transplanted.
Claims (4)
1. a water planting transplanting method for cherry plantlet in vitro, is characterized in that, specifically carries out according to the following steps:
A, to take out through culture of rootage, the bottle seedling that reaches transplanting standard from culturing room, be placed in greenhouse by solar heat, close bottle and take exercise after 5 ~ 7 days, throw off blake bottle sealing, inject running water in bottle, then temper 3 ~ 5 days;
Bottle seedling has been tempered in b, taking-up, and cleaning root, removes lower blade;
By mass fraction, water intaking 1000 parts, 0.9 part, potassium nitrate, 0.8 part, ammonium nitrate, potassium dihydrogen phosphate 0.85 part, 0.18 part, magnesium sulfate, 0.22 part, calcium chloride, manganese sulphate 0.11 part, 0.04 part, zinc sulphate and 0.18 part, ferrous sulfate respectively, mix, be made into nutrient solution;
C, get multiple hydroponic box, be placed in greenhouse, be connected successively with flexible pipe between each hydroponic box, in each hydroponic box, all place cystosepiment, this cystosepiment is processed with multiple hole of planting;
D, clean bottle seedling planted and plants in hole in cystosepiment, nutrient solution is injected in all hydroponic boxes, the nutrient solution in all hydroponic boxes is circulated;
E, with the whole hydroponic box of covered rearing with plastic film, form a closed space, Rooted Cuttings after space transition that this is closed cultivates 3 ~ 5 days, more all threw off film with 5 ~ 10 day time, ventilated and grew; Water planting 30 ~ 40 days, extracts and carries out a point cultivation;
F, by the seedling of water planting in greenhouse, individual plant move into be filled with in the nutritive cube of matrix, move in outdoor shade, make nursery stock adapt to open country growing environment gradually, afterwards, band soil mud enters land for growing field crops, manage with reference to field seedling raising method, the water planting completing cherry plantlet in vitro is transplanted.
2. the water planting transplanting method of cherry plantlet in vitro according to claim 1, is characterized in that: in described step c, the thickness of cystosepiment is 0.3 ~ 1cm.
3. the water planting transplanting method of cherry plantlet in vitro according to claim 1, is characterized in that: in described step f, 1 ︰ 0.3, fetches earth respectively and decomposed manure, mix in mass ratio, forms matrix.
4. the water planting transplanting method of cherry plantlet in vitro according to claim 1, is characterized in that: in described step f, and when seedling grows to highly for 10cm in nutritive cube, band soil mud enters land for growing field crops.
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| CN201510562058.XA CN105123479A (en) | 2015-09-07 | 2015-09-07 | Water culture transplanting method for cherry tissue culture seedlings |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105580619A (en) * | 2015-12-15 | 2016-05-18 | 铜仁学院 | Acclimatization and transplantation method for talinum crassifolium aseptic seedlings |
| CN105900662A (en) * | 2016-05-12 | 2016-08-31 | 山东理工大学 | Soilless half-hydroponic planting method of organic Chinese chives |
| CN106631276A (en) * | 2016-12-21 | 2017-05-10 | 河南红枫种苗股份有限公司 | Hydroponic domestication method of euonymus japonicus tissue culture seedlings |
| CN106804365A (en) * | 2017-02-18 | 2017-06-09 | 天长市圣丰生态农业科技有限公司 | A kind of winter resistance is strong, downgrade the car li seedling propagation breeding method of precocity fruiting |
| CN108464207A (en) * | 2018-04-13 | 2018-08-31 | 中国水稻研究所 | A kind of cultural method improving rice tissue-cultured seedling transplanting survival rate |
| CN110999786A (en) * | 2019-12-02 | 2020-04-14 | 西北农林科技大学 | Pear tissue culture seedling rooting and domestication method |
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| CN103988762A (en) * | 2014-04-18 | 2014-08-20 | 天水市果树研究所 | Water culturing and transplanting method for apple tissue culture seedlings |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105580619A (en) * | 2015-12-15 | 2016-05-18 | 铜仁学院 | Acclimatization and transplantation method for talinum crassifolium aseptic seedlings |
| CN105580619B (en) * | 2015-12-15 | 2018-07-06 | 铜仁学院 | A kind of acclimatization and transplants method of Talinum crassifolium aseptic seedling |
| CN105900662A (en) * | 2016-05-12 | 2016-08-31 | 山东理工大学 | Soilless half-hydroponic planting method of organic Chinese chives |
| CN106631276A (en) * | 2016-12-21 | 2017-05-10 | 河南红枫种苗股份有限公司 | Hydroponic domestication method of euonymus japonicus tissue culture seedlings |
| CN106804365A (en) * | 2017-02-18 | 2017-06-09 | 天长市圣丰生态农业科技有限公司 | A kind of winter resistance is strong, downgrade the car li seedling propagation breeding method of precocity fruiting |
| CN108464207A (en) * | 2018-04-13 | 2018-08-31 | 中国水稻研究所 | A kind of cultural method improving rice tissue-cultured seedling transplanting survival rate |
| CN110999786A (en) * | 2019-12-02 | 2020-04-14 | 西北农林科技大学 | Pear tissue culture seedling rooting and domestication method |
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