CN103988762B - A kind of water planting transplanting method of Tissue-cultured apple seedling - Google Patents
A kind of water planting transplanting method of Tissue-cultured apple seedling Download PDFInfo
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- CN103988762B CN103988762B CN201410156599.8A CN201410156599A CN103988762B CN 103988762 B CN103988762 B CN 103988762B CN 201410156599 A CN201410156599 A CN 201410156599A CN 103988762 B CN103988762 B CN 103988762B
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Abstract
A water planting transplanting method for Tissue-cultured apple seedling, the greenhouse comprising bottle seedling of taking root practice seedling, the cleaning of plantlet in vitro, the preparation of solution culture facilities, seedling water planting of taking root, outdoor point plant management process.The present invention does not form perfect cuticula according to Tissue-cultured apple seedling its blade before bottle outlet, the physiological characteristics such as effective photosynthesis can not be carried out, use suspension culture, make plantlet in vitro under comparatively adapt circumstance condition, promote that epidermal leaf cells is reached maturity, complete the transformation from heterotroph metabolism to autotrophic type metabolism quickly, reach the object improving Tissue-cultured apple seedling transplant survival.This technology is implemented simple, and cost is low, and transplanting survival rate reaches more than 90%.
Description
Technical field
The present invention relates to field of plant tissue culture technique, specifically a kind ofly relate to the training of apple group and to take root the method that seedling water planting transplants, this transplanting method can make transplanting survival rate reach more than 90%, and management is simple, and production cost is low.
Background technology
In recent years, along with the fast development of science and technology, plant tissue culture technique has entered the production application stage, and be widely used in flowers, the large-scale rapid propagation in vitro of fruit tree, test-tube plantlet forms industrialization in international market.Tissue cultures as the important means of apple detoxification, and realizes the fast breeding of nursery stock, and replace the growth condition of common seedling more and more extensive with group training detoxic seedling, application prospect is also very wide.
Plant tissue culture detoxifying fast breeding was produced in manual control condition's next year four seasons, and obtained a large amount of group training detoxification nursery stocks at short notice.But the transplanting of plantlet in vitro, arrives outdoor indoor, transformed to natural environmental condition gradually by the low light level in bottle, constant temperature, nearly saturated humidity, gnotobasis, technical difficulty is larger.Due to the blade of differentiation and growth under high humidity, the low light level, heterotrophism condition, leaf cuticle, wax coat are undeveloped, leaf texture gap is large, palisade tissue is thin, and the conducting system of stem grows imperfection in addition, and root is without absorption function or extremely low, when transplant high ambient temperature or humidity lower time and easily cause a large amount of dehydration of blade, and root system can not absorb water supplementary effectively, therefore it is withered very easily to wilt, and easily causes the failure of transplanting.Especially transplant in common greenhouse by solar heat or plastic tunnel, by the impact of Various Seasonal temp. and humidity, transplanting effect has very large difference.
Summary of the invention
Technical problem to be solved by this invention provides a kind of water planting transplanting method improving the Tissue-cultured apple seedling of Tissue-cultured apple seedling transplanting survival rate for transplanting the poor problem of effect in prior art.
Following technical scheme is adopted for solving technical problem of the present invention:
A water planting transplanting method for Tissue-cultured apple seedling, the greenhouse comprising bottle seedling of taking root practice seedling, the cleaning of plantlet in vitro, the preparation of solution culture facilities, seedling water planting of taking root, outdoor point plant management process:
Seedling is practiced in the indoor of a, bottle seedling of taking root: take out through culture of rootage, the bottle seedling that reaches transplanting standard from culturing room, be placed in greenhouse by solar heat, close bottle and take exercise after 5 ~ 7 days, throw off blake bottle sealing, inject the high running water of 0.3-0.5cm in bottle, then temper 3 ~ 5 days, then carry out water planting;
The cleaning of b, plantlet in vitro: the bottle seedling tweezers of having taken exercise are taken out gently from bottle, wash the medium being attached to root with clear water off, remove lower blade, reduce the damage of plantlet in vitro root, stem, leaf as far as possible, after cleaning, dry the moisture content of plantlet in vitro blade surface in the shade;
The preparation of c, solution culture facilities: the dark 20cm of hydroponic box, wide 80 ~ 100cm, long 600 ~ 800cm, puts successively, passes into nutrient solution in hydroponic box, nutrient solution is placed the cystosepiment accomplishing fluently aperture of thick 0.3 ~ 1cm for fixing young plant in greenhouse; Nutrient solution is made up of water, potassium nitrate, ammonium nitrate, potassium dihydrogen phosphate, magnesium sulfate, calcium chloride, manganese sulphate, zinc sulphate, ferrous sulfate;
The water planting of d, plantlet in vitro: planting in accomplishing fluently in the cystosepiment of aperture in advance cleaning the plantlet in vitro of taking root dried well, adding the nutrient solution that 10-15cm is high in incubator, cystosepiment being floated on nutrient solution;
E, covered rearing with plastic film: incubator mouth is all covered with plastic film, make Rooted Cuttings be placed in comparatively closed space transition to cultivate 3 ~ 5 days, all film was thrown off again with 3 ~ 5 day time, to ventilate growth, when water planting 30 ~ 40 days, main root Gabor transform, hair root increases, blade turns green, time thickening, extract and carries out a point cultivation;
F, outdoor point of cultivation management: by the seedling of water planting in greenhouse, it is soil that individual plant moves into matrix: in the nutritive cube of decomposed manure 1:0.3, move in outdoor shade, timely water spray or water, nursery stock is made to adapt to open country growing environment gradually, when growing to 10-15cm Deng seedling in nutritive cube, band soil mud enters land for growing field crops, with reference to field seedling raising management by methods.
The mass ratio of each raw material dosage of described nutrient solution is: 1000 parts, water, potassium nitrate 0.8-1.0 part, ammonium nitrate 0.7-0.9 part, potassium dihydrogen phosphate 0.75-0.95 part, magnesium sulfate 0.16-0.20 part, calcium chloride 0.20-0.24 part, manganese sulphate 0.08-0.14 part, zinc sulphate 0.02-0.06 part, ferrous sulfate 0.16-0.20 part.
Be connected with supple-management between each incubator in described step c, by water pump, nutrient solution entirety circulated.
The present invention does not form perfect cuticula according to Tissue-cultured apple seedling its blade before bottle outlet, the physiological characteristics such as effective photosynthesis can not be carried out, use suspension culture, make plantlet in vitro under comparatively adapt circumstance condition, promote that epidermal leaf cells is reached maturity, complete the transformation from heterotroph metabolism to autotrophic type metabolism quickly, reach the object improving Tissue-cultured apple seedling transplant survival.This technology is implemented simple, and cost is low, and transplanting survival rate reaches more than 90%.
Embodiment
embodiment 1
Apple tissue culture technology comprise explant foundation, expand the links such as numerous cultivation, culture of rootage, transplanting, wherein:
The foundation of explant: the annual treelet terminal bud of the vigorous excellent apple tree of clip grown in field or lateral bud, use running water flow wash, 74% alcohol surface sterilization, with 0.1% mercuric chloride sterilizing, after finally clean with aseptic water washing, desinfection chamber superclean bench is received and inducing culture carries out in culturing room differentiation-inducing, obtain nontoxic stem apex as cultivation explant;
Expand numerous cultivation: the stem section nontoxic stem apex that said method obtains being cut into 2cm, desinfection chamber superclean bench is inoculated into and expands in breeding culture medium, every bottle of medium connects 4 buds, is placed on culturing room and carries out expanding numerous cultivation;
Take root: when nursery stock is expanded numerous after some, select the strong sprout of robust growth, on desinfection chamber superclean bench, being cut into 2cm stem apex is inoculated on root media, every bottle of 5 strains, is placed on culturing room and carries out culture of rootage, after the little seedling rooting of more than 90%, culturing room can be pulled out and transplant.
The greenhouse that Tissue-cultured apple seedling Greenhouse Water Culture transplantation technique comprises bottle seedling of taking root practice seedling, the cleaning of plantlet in vitro, the preparation of solution culture facilities, the configuration of nutrient solution, seedling water planting of taking root, outdoor point plant management process:
Seedling is practiced in the indoor of a, bottle seedling of taking root: take out through culture of rootage, the bottle seedling that reaches transplanting standard from culturing room, be placed in greenhouse by solar heat, closes after bottle tempers 7 days, throw off blake bottle sealing, inject the high running water of 0.3cm in bottle, then temper 3 days, then carry out water planting;
The cleaning of b, plantlet in vitro: the bottle seedling tweezers of having taken exercise are taken out gently from bottle, wash the medium being attached to root with clear water off, remove lower blade, reduce the damage of plantlet in vitro root, stem, leaf as far as possible, after cleaning, dry the moisture content of plantlet in vitro blade surface in the shade;
The preparation of c, solution culture facilities: the dark 20cm of hydroponic box, wide 80cm, long 800cm, puts successively in greenhouse, is connected, by water pump, nutrient solution entirety is circulated, nutrient solution is placed the cystosepiment of thick 1cm for fixing young plant between each incubator with supple-management;
The configuration of d, nutrient solution: nutrient solution is by being form with water, potassium nitrate, ammonium nitrate, potassium dihydrogen phosphate, magnesium sulfate, calcium chloride, manganese sulphate, zinc sulphate, ferrous sulfate, and the mass ratio of each raw material dosage is: 1000 parts, water, 0.8 part, potassium nitrate, 0.9 part, ammonium nitrate, potassium dihydrogen phosphate 0.75 part, 0.16 part, magnesium sulfate, 0.24 part, calcium chloride, manganese sulphate 0.14 part, 0.02 part, zinc sulphate, 0.16 part, ferrous sulfate;
The water planting of e, plantlet in vitro: planting in accomplishing fluently in the cystosepiment of aperture in advance cleaning the plantlet in vitro of taking root dried well, adding the nutrient solution that 10cm is high in incubator, cystosepiment being floated on nutrient solution, open water pump, Nutrient Cycle is flowed;
F, covered rearing with plastic film: incubator mouth is all covered with plastic film, make Rooted Cuttings be placed in the space transition comparatively closed and cultivate 3 days, all film was thrown off again with 3 day time, to ventilate growth, when water planting 40 days, main root Gabor transform, hair root increases, blade turns green, time thickening, extract and carries out a point cultivation;
G, outdoor point of cultivation management: by the seedling of water planting in greenhouse, it is soil that individual plant moves into matrix: in the nutritive cube of decomposed manure 1:0.3, move in outdoor shade, timely water spray or water, nursery stock is made to adapt to open country growing environment gradually, when growing to 10cm Deng seedling in nutritive cube, band soil mud enters land for growing field crops, with reference to field seedling raising management by methods.
embodiment 2
Apple tissue culture technology comprise explant foundation, expand the links such as numerous cultivation, culture of rootage, transplanting, wherein:
The foundation of explant: the annual treelet terminal bud of the vigorous excellent apple tree of clip grown in field or lateral bud, use running water flow wash, 74% alcohol surface sterilization, with 0.1% mercuric chloride sterilizing, after finally clean with aseptic water washing, desinfection chamber superclean bench is received and inducing culture carries out in culturing room differentiation-inducing, obtain nontoxic stem apex as cultivation explant;
Expand numerous cultivation: the stem section nontoxic stem apex that said method obtains being cut into 2cm, desinfection chamber superclean bench is inoculated into and expands in breeding culture medium, every bottle of medium connects 3 buds, is placed on culturing room and carries out expanding numerous cultivation;
Take root: when nursery stock is expanded numerous after some, select the strong sprout of robust growth, on desinfection chamber superclean bench, being cut into 2cm stem apex is inoculated on root media, every bottle of 4 strains, is placed on culturing room and carries out culture of rootage, after the little seedling rooting of more than 90%, culturing room can be pulled out and transplant.
The greenhouse that Tissue-cultured apple seedling Greenhouse Water Culture transplantation technique comprises bottle seedling of taking root practice seedling, the cleaning of plantlet in vitro, the preparation of solution culture facilities, the configuration of nutrient solution, seedling water planting of taking root, outdoor point plant management process:
Seedling is practiced in the indoor of a, bottle seedling of taking root: take out through culture of rootage, the bottle seedling that reaches transplanting standard from culturing room, be placed in greenhouse by solar heat, closes after bottle tempers 5 days, throw off blake bottle sealing, inject the high running water of 0.5cm in bottle, then temper 5 days, then carry out water planting;
The cleaning of b, plantlet in vitro: the bottle seedling tweezers of having taken exercise are taken out gently from bottle, wash the medium being attached to root with clear water off, remove lower blade, reduce the damage of plantlet in vitro root, stem, leaf as far as possible, after cleaning, dry the moisture content of plantlet in vitro blade surface in the shade;
The preparation of c, solution culture facilities: the dark 20cm of hydroponic box, wide 100cm, long 600cm, put successively in greenhouse, be connected with supple-management between each incubator, by water pump, nutrient solution entirety circulated, nutrient solution is placed the cystosepiment of thick 0.3cm for fixing young plant;
The configuration of d, nutrient solution: nutrient solution is by being form with water, potassium nitrate, ammonium nitrate, potassium dihydrogen phosphate, magnesium sulfate, calcium chloride, manganese sulphate, zinc sulphate, ferrous sulfate, and the mass ratio of each raw material dosage is: 1000 parts, water, 1.0 parts, potassium nitrate, 0.7 part, ammonium nitrate, potassium dihydrogen phosphate 0.95 part, 0.20 part, magnesium sulfate, 0.20 part, calcium chloride, manganese sulphate 0.08 part, 0.06 part, zinc sulphate, 0.20 part, ferrous sulfate;
The water planting of e, plantlet in vitro: planting in accomplishing fluently in the cystosepiment of aperture in advance cleaning the plantlet in vitro of taking root dried well, adding the nutrient solution that 15cm is high in incubator, cystosepiment being floated on nutrient solution, open water pump, Nutrient Cycle is flowed;
F, covered rearing with plastic film: incubator mouth is all covered with plastic film, make Rooted Cuttings be placed in the space transition comparatively closed and cultivate 5 days, all film was thrown off again with 5 day time, to ventilate growth, when water planting 30 days, main root Gabor transform, hair root increases, blade turns green, time thickening, extract and carries out a point cultivation;
G, outdoor point of cultivation management: by the seedling of water planting in greenhouse, it is soil that individual plant moves into matrix: in the nutritive cube of decomposed manure 1:0.3, move in outdoor shade, timely water spray or water, nursery stock is made to adapt to open country growing environment gradually, when growing to 15cm Deng seedling in nutritive cube, band soil mud enters land for growing field crops, with reference to field seedling raising management by methods.
Claims (3)
1. a water planting transplanting method for Tissue-cultured apple seedling, it is characterized in that comprising take root the greenhouse of bottle seedling practice seedling, the cleaning of plantlet in vitro, the preparation of solution culture facilities, seedling water planting of taking root, outdoor point plant management process:
Seedling is practiced in the indoor of a, bottle seedling of taking root: take out through culture of rootage, the bottle seedling that reaches transplanting standard from culturing room, be placed in greenhouse by solar heat, close bottle and take exercise after 5 ~ 7 days, throw off blake bottle sealing, inject the high running water of 0.3-0.5cm in bottle, then temper 3 ~ 5 days, then carry out water planting;
The cleaning of b, plantlet in vitro: the bottle seedling tweezers of having taken exercise are taken out gently from bottle, wash the medium being attached to root with clear water off, remove lower blade, reduce the damage of plantlet in vitro root, stem, leaf as far as possible, after cleaning, dry the moisture content of plantlet in vitro blade surface in the shade;
The preparation of c, solution culture facilities: the dark 20cm of incubator, wide 80 ~ 100cm, long 600 ~ 800cm, puts successively, passes into nutrient solution in incubator, nutrient solution is placed the cystosepiment accomplishing fluently aperture of thick 0.3 ~ 1cm for fixing young plant in greenhouse; Nutrient solution is made up of water, potassium nitrate, ammonium nitrate, potassium dihydrogen phosphate, magnesium sulfate, calcium chloride, manganese sulphate, zinc sulphate, ferrous sulfate;
The water planting of d, plantlet in vitro: planting in accomplishing fluently in the cystosepiment of aperture in advance cleaning the plantlet in vitro of taking root dried well, adding the nutrient solution that 10cm-15cm is high in incubator, cystosepiment being floated on nutrient solution;
E, covered rearing with plastic film: incubator mouth is all covered with plastic film, make Rooted Cuttings be placed in comparatively closed space transition to cultivate 3 ~ 5 days, all film was thrown off again with 3 ~ 5 day time, to ventilate growth, when water planting 30 ~ 40 days, main root Gabor transform, hair root increases, blade turns green, time thickening, extract and carries out a point cultivation;
F, outdoor point of cultivation management: by the seedling of water planting in greenhouse, it is soil that individual plant moves into matrix: in the nutritive cube of decomposed manure 1:0.3, move in outdoor shade, timely water spray or water, nursery stock is made to adapt to open country growing environment gradually, soil mud is with to enter land for growing field crops when growing to 10-15cm Deng seedling in nutritive cube, with reference to field seedling raising management by methods.
2. the water planting transplanting method of a kind of Tissue-cultured apple seedling according to claim 1, is characterized in that: the mass ratio of each raw material dosage of described nutrient solution is: 1000 parts, water, potassium nitrate 0.8-1.0 part, ammonium nitrate 0.7-0.9 part, potassium dihydrogen phosphate 0.75-0.95 part, magnesium sulfate 0.16-0.20 part, calcium chloride 0.20-0.24 part, manganese sulphate 0.08-0.14 part, zinc sulphate 0.02-0.06 part, ferrous sulfate 0.16-0.20 part.
3. the water planting transplanting method of a kind of Tissue-cultured apple seedling according to claim 1 and 2, is characterized in that: be connected with flexible pipe between each incubator in described step c, by water pump, nutrient solution entirety circulated.
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| CN104335887A (en) * | 2014-10-14 | 2015-02-11 | 金陵科技学院 | Method for increasing transplanting survival rate of tissue culture hybrid seedlings of orchid |
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| CN106212237B (en) * | 2016-08-08 | 2021-05-04 | 天水市果树研究所 | Ex-bottle rooting method for apple tissue culture seedlings |
| CN110679457B (en) * | 2019-11-14 | 2022-04-08 | 中国林业科学研究院亚热带林业研究所 | Method for improving transplanting survival rate of tissue culture seedlings of Nicotiana benthamiana |
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