CN104026021B - Tulip tissue culture and rapid propagation method - Google Patents
Tulip tissue culture and rapid propagation method Download PDFInfo
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- CN104026021B CN104026021B CN201410288468.5A CN201410288468A CN104026021B CN 104026021 B CN104026021 B CN 104026021B CN 201410288468 A CN201410288468 A CN 201410288468A CN 104026021 B CN104026021 B CN 104026021B
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Abstract
The invention discloses a kind of tulip tissue culture and rapid propagation method, belong to field of plant tissue culture technique, concrete steps are: the band bud scape choosing tulip plant, cut into segment, sterilization, be seeded to bud inducement medium, regulating illumination intensity and temperature, be cultured to and grow sprouting, then turn and be inoculated in strong seedling culture base, regulating illumination intensity and temperature, cultivate to obtain strong sprout, transfer into root media, regulating illumination is around temperature, cultivation obtains tulip plantlet in vitro, transplant and carry out hardening cultivation to seedling-cultivating tray, control air humidity is, regulating illumination intensity, hardening is cultivated, finally transplant to open country and cultivate.Present invention reduces the breeding cycle of tulip.
Description
Technical field
The present invention relates to field of plant tissue culture technique, especially a kind of tulip tissue culture and rapid propagation method.
Background technology
Tulip, belongs to long-day flowers, and property happiness faces south, wind sheltering, is suitable for warm and moist in winter, summer feels nice and cool dry weather.Tulip not only has higher ornamental value, and has treatment taste and wet turbid, chest distress, inverse stomachache of vomitting, effect of the greasy grade of halitosis tongue.Traditional modes of reproduction of tulip is bulb separation breeding, and its reproduction speed is slow, and new varieties put the time needing about 20 years on market, and the infecting of susceptible viral in cultivation, causes its deterioration of variety serious.So, be badly in need of seeking a kind of high yield, high-quality, efficiently propagation method.
Summary of the invention
The object of this invention is to provide a kind of tulip tissue culture and rapid propagation method, this tulip tissue culture and rapid propagation method can solve the slow problem of tulip reproduction speed.
In order to solve the problem, the technical solution used in the present invention is: a kind of tulip tissue culture and rapid propagation method, comprises the following steps:
A, choose the band bud scape of healthy and strong tulip plant, cut into the segment of 1 centimetre ~ 1.5 centimetres, with alcohol-pickled 30 seconds ~ 60 seconds of 75%, aseptic water washing 3 times ~ 5 times;
B, be seeded to bud inducement medium, regulating illumination 2200LUX ~ 2500LUX, temperature is 23 DEG C ~ 26 DEG C, cultivates 8 days ~ 10 days to growing sprouting; Wherein, described bud inducement medium for minimal medium, adds 0.25 mg/litre ~ 0.3 mg/litre methyl α-naphthyl acetate and 0.3 mg/litre ~ 0.4 mg/litre 6-benzyl aminoadenine with MS medium;
C, when described sprouting grows to 1 centimetre ~ 2 centimetres, be inoculated in strong seedling culture base, regulating illumination 2300LUX ~ 2500LUX, temperature is 23 DEG C ~ 25 DEG C, cultivates 13 days ~ 18 days to height of seedling 1 centimetre ~ 1.5 centimetres; Wherein, described strong seedling culture base for minimal medium, adds 0.25 mg/litre ~ 0.3 mg/litre methyl α-naphthyl acetate with 1/2MS medium;
D, transfer into root media by above-mentioned strong sprout, regulating illumination 2200Lux ~ 2500Lux, temperature is 23 DEG C ~ 26 DEG C, cultivates the root growing 1 centimetre ~ 4 centimetres for 15 days ~ 20 days to seedling base portion, obtains tulip plantlet in vitro; Described foster base of taking root for minimal medium, adds 0.25 mg/litre ~ 0.3 mg/litre methyl α-naphthyl acetate and 0.1 mg/litre ~ 0.2 mg/litre gibberellin with 1/2MS medium;
E, when the root of described tulip plantlet in vitro grows to 0.5 centimetre ~ 1 centimetre, tulip plantlet in vitro is transplanted to vermiculite is housed, carry out hardening cultivation in the seedling-cultivating tray of the peat composed of rotten mosses and sawdust, controlling air humidity is 85% ~ 90%, and regulating illumination intensity is 10000Lux ~ 15000Lux; Wherein, vermiculite, the peat composed of rotten mosses, the mass ratio of sawdust is 1:2:2;
F, hardening are transplanted to open country and cultivate after cultivating 30 days ~ 45 days.
Owing to have employed technique scheme, the present invention compared with prior art has following beneficial effect:
The present invention carries out tissue cultures with the band bud scape of tulip plant for material, substantially reduces the breeding cycle of tulip, and gained seedling early growth is healthy and strong, and is not subject to seasonal restrictions, can spread.
Embodiment
Below in conjunction with embodiment, the invention will be further described:
Embodiment 1
Choose the band bud scape of healthy and strong tulip plant, cut into the segment of 1 centimetre, with alcohol-pickled 30 seconds of 75%, aseptic water washing 5 times; Described segment being seeded to bud inducement medium, regulating illumination 2200LUX, adjusting the temperature to 23 DEG C, cultivating 10 days to growing sprouting;
When described sprouting grows to 1 centimetre, be inoculated in strong seedling culture base, regulating illumination 2300Lux, temperature is 23 DEG C, cultivates 18 days to height of seedling 1.5 centimetres; Transfer into root media by above-mentioned strong sprout, regulating illumination 2200Lux, temperature is 23 DEG C, cultivates the root growing 4 centimetres for 20 days to seedling base portion, obtains tulip plantlet in vitro; When the root of described tulip plantlet in vitro grows to 0.5 centimetre, transplant tulip plantlet in vitro to vermiculite, the peat composed of rotten mosses, the mass ratio of sawdust is carry out hardening cultivation in the seedling-cultivating tray of 1:2:2, and controlling air humidity is 85%, and regulating illumination intensity is 10000Lux; After hardening cultivates 30 days, be transplanted to open country and cultivate.
In the present embodiment bud inducement medium be with MS medium for minimal medium, add 0.25 mg/litre methyl α-naphthyl acetate and 0.4 mg/litre 6-benzyl aminoadenine; Strong seedling culture base for minimal medium, adds 0.3 mg/litre methyl α-naphthyl acetate with 1/2MS medium; Take root foster base with 1/2MS medium for minimal medium, adds 0.25 mg/litre methyl α-naphthyl acetate and 0.1 mg/litre gibberellin.
Embodiment 2
Choose the band bud scape of healthy and strong tulip plant, cut into the segment of 1.5 centimetres, with alcohol-pickled 60 seconds of 75%, aseptic water washing 3 times; Described segment being seeded to bud inducement medium, regulating illumination 2500LUX, adjusting the temperature to 26 DEG C, cultivating 8 days to growing sprouting; When described sprouting grows to 2 centimetres, be inoculated in strong seedling culture base, regulating illumination 2500Lux, temperature is 25 DEG C, cultivates 13 days to height of seedling 1 centimetre; Transfer into root media by above-mentioned strong sprout, regulating illumination 2500Lux, temperature is 26 DEG C, cultivates the root growing 1 centimetre for 15 days to seedling base portion, obtains tulip plantlet in vitro; When the root of described tulip plantlet in vitro grows to 1 centimetre, transplant tulip plantlet in vitro to vermiculite, the peat composed of rotten mosses, the mass ratio of sawdust is carry out hardening cultivation in the seedling-cultivating tray of 1:2:2, and controlling air humidity is 90%, and regulating illumination intensity is 15000Lux; After hardening cultivates 45 days, be transplanted to open country and cultivate.
In the present embodiment bud inducement medium be with MS medium for minimal medium, add 0.3 mg/litre methyl α-naphthyl acetate and 0.3 mg/litre 6-benzyl aminoadenine; Strong seedling culture base for minimal medium, adds 0.25 mg/litre methyl α-naphthyl acetate with 1/2MS medium; Take root foster base with 1/2MS medium for minimal medium, adds 0.3 mg/litre methyl α-naphthyl acetate and 0.2 mg/litre gibberellin.
Embodiment 3
Choose the band bud scape of healthy and strong tulip plant, cut into the segment of 1.5 centimetres, with alcohol-pickled 45 seconds of 75%, aseptic water washing 4 times; Described segment being seeded to bud inducement medium, regulating illumination 2400LUX, adjusting the temperature to 25 DEG C, cultivating 10 days to growing sprouting; When described sprouting grows to 2 centimetres, be inoculated in strong seedling culture base, regulating illumination 2300Lux, temperature is 25 DEG C, cultivates 15 days to height of seedling 1 centimetre; Transfer into root media by above-mentioned strong sprout, regulating illumination 2400Lux, temperature is 25 DEG C, cultivates the root growing 1 centimetre for 18 days to seedling base portion, obtains tulip plantlet in vitro; When the root of described tulip plantlet in vitro grows to 1 centimetre, transplant tulip plantlet in vitro to vermiculite, the peat composed of rotten mosses, the mass ratio of sawdust is carry out hardening cultivation in the seedling-cultivating tray of 1:2:2, and controlling air humidity is 90%, and regulating illumination intensity is 12000Lux; After hardening cultivates 40 days, be transplanted to open country and cultivate.
In the present embodiment bud inducement medium be with MS medium for minimal medium, add 0.3 mg/litre methyl α-naphthyl acetate and 0.35 mg/litre 6-benzyl aminoadenine; Strong seedling culture base for minimal medium, adds 0.3 mg/litre methyl α-naphthyl acetate with 1/2MS medium; Take root foster base with 1/2MS medium for minimal medium, adds 0.3 mg/litre methyl α-naphthyl acetate and 0.15 mg/litre gibberellin.
Claims (1)
1. a tulip tissue culture and rapid propagation method, is characterized in that comprising the following steps:
A, choose the band bud scape of healthy and strong tulip plant, cut into the segment of 1 centimetre ~ 1.5 centimetres, with alcohol-pickled 30 seconds ~ 60 seconds of 75%, aseptic water washing 3 times ~ 5 times;
B, be seeded to bud inducement medium, regulating illumination 2200LUX ~ 2500LUX, temperature is 23 DEG C ~ 26 DEG C, cultivates 8 days ~ 10 days to growing sprouting; Wherein, described bud inducement medium for minimal medium, adds 0.25 mg/litre ~ 0.3 mg/litre methyl α-naphthyl acetate and 0.3 mg/litre ~ 0.4 mg/litre 6-benzyl aminoadenine with MS medium;
C, when described sprouting grows to 1 centimetre ~ 2 centimetres, be inoculated in strong seedling culture base, regulating illumination 2300LUX ~ 2500LUX, temperature is 23 DEG C ~ 25 DEG C, cultivates 13 days ~ 18 days to height of seedling 1 centimetre ~ 1.5 centimetres; Wherein, described strong seedling culture base for minimal medium, adds 0.25 mg/litre ~ 0.3 mg/litre methyl α-naphthyl acetate with 1/2MS medium;
D, transfer into root media by above-mentioned strong sprout, regulating illumination 2200Lux ~ 2500Lux, temperature is 23 DEG C ~ 26 DEG C, cultivates the root growing 1 centimetre ~ 4 centimetres for 15 days ~ 20 days to seedling base portion, obtains tulip plantlet in vitro; Described root media for minimal medium, adds 0.25 mg/litre ~ 0.3 mg/litre methyl α-naphthyl acetate and 0.1 mg/litre ~ 0.2 mg/litre gibberellin with 1/2MS medium;
E, when the root of described tulip plantlet in vitro grows to 0.5 centimetre ~ 1 centimetre, tulip plantlet in vitro is transplanted to vermiculite is housed, carry out hardening cultivation in the seedling-cultivating tray of the peat composed of rotten mosses and sawdust, controlling air humidity is 85% ~ 90%, and regulating illumination intensity is 10000Lux ~ 15000Lux; Wherein, vermiculite, the peat composed of rotten mosses, the mass ratio of sawdust is 1:2:2;
F, hardening cultivate 30 days ~ 45 days, and the tulip plantlet in vitro transplanting after then hardening being cultivated is cultivated to open country.
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| CN105359874A (en) * | 2015-10-31 | 2016-03-02 | 余大春 | Pinelliacordata seedling culture medium and seedling culture method |
| CN107372123B (en) * | 2017-09-13 | 2019-06-11 | 界首市家丰家庭农场 | A method of improving tulip flourish |
| CN115843682B (en) * | 2022-10-28 | 2024-02-27 | 华中农业大学 | Method for inducing and regenerating tulip hypocotyl callus |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101953300A (en) * | 2010-08-24 | 2011-01-26 | 浙江大学 | Tissue culture method for Curcuma wenyujin No.1 |
| CN102090331A (en) * | 2010-11-10 | 2011-06-15 | 天津滨海国际花卉科技园区股份有限公司 | Method for enhancing tulip tissue culture seedling |
| CN102907325A (en) * | 2012-11-06 | 2013-02-06 | 广东第二师范学院 | Method for utilizing culture technology to produce Solomon turmeric and red tulip seedlings |
| CN103238523A (en) * | 2013-05-31 | 2013-08-14 | 常熟市佳盛农业科技发展有限公司 | Method for rapidly breeding tulip by using tulip scales |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101953300A (en) * | 2010-08-24 | 2011-01-26 | 浙江大学 | Tissue culture method for Curcuma wenyujin No.1 |
| CN102090331A (en) * | 2010-11-10 | 2011-06-15 | 天津滨海国际花卉科技园区股份有限公司 | Method for enhancing tulip tissue culture seedling |
| CN102907325A (en) * | 2012-11-06 | 2013-02-06 | 广东第二师范学院 | Method for utilizing culture technology to produce Solomon turmeric and red tulip seedlings |
| CN103238523A (en) * | 2013-05-31 | 2013-08-14 | 常熟市佳盛农业科技发展有限公司 | Method for rapidly breeding tulip by using tulip scales |
Non-Patent Citations (3)
| Title |
|---|
| effects of thidiazuron and paclobutrazol on regeneration potential of tulip flower stalk explants in vitro and subsequent shoot multiplication;Malgorzata podwyszynska,et al.;《ACTA SOCIETATIS BOTANICORUM POLONIAE》;20031231;第72卷(第3期);第181-190页 * |
| 我国郁金香组织培养的研究进展;曹前进等;《上海农业科技》;20031231;第9页左栏第2-4段、右栏第5段，第10页倒数第2段 * |
| 郁金香的组织培养;谢亚红等;《植物生理学通讯》;19851231(第4期);第36页左栏第1-3段 * |
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Address after: Cheung Chau Fengshan County town of Hechi city the Guangxi Zhuang Autonomous Region 547600 Old Village Development Fund Association Patentee after: Chen Qiaolan Address before: 545007 the Guangxi Zhuang Autonomous Region District of Liuzhou city road two Xingbin District 24 Building 1 unit 302 room Patentee before: Chen Qiaolan |
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Effective date of registration: 20201119 Address after: 535000 3 / F, building 1, Qinzhou nixing pottery cultural and Creative Industrial Park (Millennium ancient pottery city), Qinzhou City, Guangxi Zhuang Autonomous Region Patentee after: Qinzhou Qinnan Jinwo Industrial Park Management Committee Address before: Cheung Chau Fengshan County town of Hechi city the Guangxi Zhuang Autonomous Region 547600 Old Village Development Fund Association Patentee before: Chen Qiaolan |