CN103238523A - Method for rapidly breeding tulip by using tulip scales - Google Patents
Method for rapidly breeding tulip by using tulip scales Download PDFInfo
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Abstract
The invention discloses a method for rapidly breeding tulip by using tulip scales. The method specifically comprises the steps of selecting plants, refrigerating, disinfecting, cutting the scales, carrying out bud forcing treatment on the scales, carrying out expansion treatment on the scales, transplanting seed balls, and the like, wherein the large tulip seed balls are firstly refrigerated before being cut, thereby achieving the remarkable effects on improving the breeding coefficients and cultivating strong seedlings; and by coating GA3 and NAA (Nicotinic Acid Amide) on small differentiated tulip scales to prompt the scales to differentiate, the time is greatly shortened, and compared with the conventional method, about one month is saved, so that the labor and the cost are reduced.
Description
Technical field
The present invention relates to the propagation method of a kind of tulip, be specifically related to utilize the method for the young ball of scale quickly breeding tulip.
Background technology
Tulip claims foreign lotus, careless Moschus again, and tulip originates in Iran and Turkey alpine belt.Be the perennial napiform root herbage flower of Liliaceae.By the bulb bulb separation breeding of routine, can only breed 3-5 every year doubly, reproduction speed is extremely slow, and because virus infections (especially mosaic virus and broken look virus) makes deterioration of variety, is difficult to satisfy the production needs.In the production process of tulip commodity ball, tulip that need be a large amount of produces with planting, and plants though production uses kind of the filial generation that can produce equal specification to produce to use, owing to breed difference, the difference of reproduction coefficient, plant the degeneration that ball produces, along with the increase of algebraically, filial generation is produced with kind of a nodule number amount meeting and is sharply reduced, the kind merit is decline constantly, expand the scale of production and stable commodity ball quality, set up the stock breeding system, constantly the production high-quality is produced with kind of a ball and is seemed especially important; China is increasing to the demand of tulip, the annual tulip main dependence on import of originating, and the tulip of China produces can not satisfy the demand in market far away.
The conventional propagation method of tulip is the plant division clove, but limited amount; Some kind can be used the scale cuttage, but often rots easily, when crossbreeding, carry out seed propagation, breeding cycle is long, coefficient is low, speed is slow, and the research of tulip seed balls breeding is also arranged at present, but research exists relatively long problem of the time of breeding always.
Summary of the invention
The technical problem that the present invention mainly solves provides a kind of method of utilizing the tulip scale to breed tulip fast, can breed tulip in the short time when saving the tulip resource, reaches purpose fast and efficiently.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is:
A kind of method of utilizing the tulip scale to breed tulip fast is characterized in that, may further comprise the steps:
(1) select that freshness height, vitality are strong, the big tulip seed balls of well-grown, growing way unanimity, utilize 1.2% potassium nitrate, 0.20% thiocarbamide, 600mg/L GA
3, 450 mg/L ethrel handle to remove the dormancy of tulip;
(2) refrigeration is handled: tulip seed balls is mixed being placed in the plastic sack with silt, fill with the liver moss after the sterilization around the bulb, humidity about 65% ties plastic sack then, and the dislocation refrigerator-freezer is handled 20 d down for 0 ℃;
(3) disinfect: will at first rinse well with clear water through the kind ball that refrigeration is handled, and plant ball 20 min, aseptic water washing 2-3 time with 10% tertiary sodium phosphate immersion again; Scale with tulip separates with the stem section then, selects the material of no scab, and with 70% alcohol disinfecting 30s, aseptic water washing 2-3 time is again with 5% liquor natrii hypochloritis, 10 min that sterilize, aseptic water washing 2-3 time; At last with 0.1% mercuric chloride solution, 10 min that sterilize, aseptic water washing 4-5 time;
(4) scale cutting: the tulip bulb of handling is at first removed scale top, account for the 1/4-1/3 of whole bulb greatly, then the bulb of middle part and bottom vertically is cut into 8 or 16 strippings and slicings, more every stripping and slicing rip cutting is become to have the fritter of 1-3 scale and plateau;
(5) scale vernalization is handled: culture matrix (is comprised the peat composed of rotten mosses, garden mould, vermiculite, humus soil, four ratio is 1:2:1:2) utilize 1 000 times of liquid of 75% tpn to carry out spray disinfectant, soup is advisable to go deep into matrix 1-2cm, and control matrix water content is 50-60%; Matrix is carried out embedding to scale section, under 20-25 ℃ and dark condition vernalization 20-30 days, during keep the matrix water content at 50-60%, air humidity is controlled at 60-70%, differentiates ramentum at the tulip scale;
(6) scale expands processing: smear 200-300mg/L GA at the ramentum that differentiation is come out
3Impel the differentiation of scale with the NAA of 100-200 mg/L, after ramentum that differentiation is come out reaches more than 90%, continuation is in temperature is 20-25 ℃ dark surrounds, carrying out scale expands and handled 10-30 days, keeping water supply in media content during this time is 60-70%, air humidity control forms the young ball of tulip gradually at 60-70%;
(7) young ball is transplanted: the young ball that will grow up to 15 min that sterilize in potassium permanganate 0.1 % soup transplant then, manage according to conventional method.
The invention has the beneficial effects as follows:
(1) tulip macrospecies ball is at first refrigerated processing before carrying out cutting process, for improving reproduction coefficient, cultivating remarkable result is all arranged strong sprout.
(2) tulip is carried out the scale cutting process and breed young ball, can save the tulip resource greatly, thereby reduce the cultivation cost greatly.
(3) the tulip ramentum that comes out in differentiation is smeared GA
3Impel the differentiation of scale with NAA, the time that has shortened greatly, can save the time of a wheat harvesting period than traditional method, thereby reduce manpower and financial resources.
Embodiment
Below preferred embodiment of the present invention is described in detail, thereby so that advantages and features of the invention can be easier to be it will be appreciated by those skilled in the art that protection scope of the present invention is made more explicit defining.
Embodiment 1
A kind of method of utilizing the tulip scale to breed tulip fast is characterized in that, may further comprise the steps:
(1) select that freshness height, vitality are strong, the big tulip seed balls of well-grown, growing way unanimity, utilize 1.2% potassium nitrate, 0.20% thiocarbamide, 600mg/L GA
3, 450 mg/L ethrel handle to remove the dormancy of tulip;
(2) refrigeration is handled: tulip seed balls is mixed being placed in the plastic sack with silt, fill with the liver moss after the sterilization around the bulb, humidity about 65% ties plastic sack then, and the dislocation refrigerator-freezer is handled 20 d down for 0 ℃;
(3) disinfect: will at first rinse well with clear water through the kind ball that refrigeration is handled, and plant ball 20 min, aseptic water washing 2 times with 10% tertiary sodium phosphate immersion again; Scale with tulip separates with the stem section then, selects the material of no scab, and with 70% alcohol disinfecting 30s, aseptic water washing 3 times is again with 5% liquor natrii hypochloritis, 10 min that sterilize, aseptic water washing 3 times; At last with 0.1% mercuric chloride solution, 10 min that sterilize, aseptic water washing 4 times;
(4) scale cutting: the tulip bulb of handling is at first removed scale top, account for 1/4 of whole bulb greatly, then the bulb of middle part and bottom vertically is cut into 8 strippings and slicings, more every stripping and slicing rip cutting is become to have the fritter of 1 scale and plateau;
(5) scale vernalization is handled: culture matrix (is comprised the peat composed of rotten mosses, garden mould, vermiculite, humus soil, four ratio is 1:2:1:2) utilize 1000 times of liquid of 75% tpn to carry out spray disinfectant, soup is advisable to go deep into matrix 1-2cm, and control matrix water content is 50%; Matrix is carried out embedding to scale section, and vernalization is 30 days under 20 ℃ and dark condition, during keep the matrix water content 50%, air humidity is controlled 60%, differentiates ramentum at the tulip scale;
(6) scale expands processing: smear 200mg/L GA at the ramentum that differentiation is come out
3Impel the differentiation of scale with the NAA of 200 mg/L, after ramentum that differentiation is come out reaches more than 90%, continuation is in temperature is 20 ℃ dark surrounds, carrying out scale expands and handled 10-30 days, keeping water supply in media content during this time is 60%, air humidity control forms the young ball of tulip gradually 60%;
(7) young ball is transplanted: the young ball that will grow up to 15 min that sterilize in potassium permanganate 0.1 % soup transplant then, manage according to conventional method.
Embodiment 2
A kind of method of utilizing the tulip scale to breed tulip fast is characterized in that, may further comprise the steps:
(1) select that freshness height, vitality are strong, the big tulip seed balls of well-grown, growing way unanimity, utilize 1.2% potassium nitrate, 0.20% thiocarbamide, 600mg/L GA
3, 450 mg/L ethrel handle to remove the dormancy of tulip;
(2) refrigeration is handled: tulip seed balls is mixed being placed in the plastic sack with silt, fill with the liver moss after the sterilization around the bulb, humidity about 65% ties plastic sack then, and the dislocation refrigerator-freezer is handled 20 d down for 0 ℃;
(3) disinfect: will at first rinse well with clear water through the kind ball that refrigeration is handled, and plant ball 20 min, aseptic water washing 2 times with 10% tertiary sodium phosphate immersion again; Scale with tulip separates with the stem section then, selects the material of no scab, and with 70% alcohol disinfecting 30s, aseptic water washing 3 times is again with 5% liquor natrii hypochloritis, 10 min that sterilize, aseptic water washing 3 times; At last with 0.1% mercuric chloride solution, 10 min that sterilize, aseptic water washing 5 times;
(4) scale cutting: the tulip bulb of handling is at first removed scale top, account for 1/3 of whole bulb greatly, then the bulb of middle part and bottom vertically is cut into 16 strippings and slicings, more every stripping and slicing rip cutting is become to have the fritter of 2 scales and plateau;
(5) scale vernalization is handled: culture matrix (is comprised the peat composed of rotten mosses, garden mould, vermiculite, humus soil, four ratio is 1:2:1:2) utilize 1000 times of liquid of 75% tpn to carry out spray disinfectant, soup is advisable to go deep into matrix 1-2cm, and control matrix water content is 55%; Matrix is carried out embedding to scale section, and vernalization is 25 days under 22 ℃ and dark condition, during keep the matrix water content 55%, air humidity is controlled 65%, differentiates ramentum at the tulip scale;
(6) scale expands processing: smear 250mg/L GA at the ramentum that differentiation is come out
3Impel the differentiation of scale with the NAA of 150 mg/L, after ramentum that differentiation is come out reached 90% or more, continuation was in temperature is 22 ℃ dark surrounds, carrying out scale expands and handled 20 days, keeping water supply in media content during this time is 65%, and air humidity is controlled 65%, forms the young ball of tulip gradually;
(7) young ball is transplanted: the young ball that will grow up to 15 min that sterilize in potassium permanganate 0.1 % soup transplant then, manage according to conventional method.
Embodiment 3
A kind of method of utilizing the tulip scale to breed tulip fast is characterized in that, may further comprise the steps:
(1) select that freshness height, vitality are strong, the big tulip seed balls of well-grown, growing way unanimity, utilize 1.2% potassium nitrate, 0.20% thiocarbamide, 600mg/L GA
3, 450 mg/L ethrel handle to remove the dormancy of tulip;
(2) refrigeration is handled: tulip seed balls is mixed being placed in the plastic sack with silt, fill with the liver moss after the sterilization around the bulb, humidity about 65% ties plastic sack then, and the dislocation refrigerator-freezer is handled 20 d down for 0 ℃;
(3) disinfect: will at first rinse well with clear water through the kind ball that refrigeration is handled, and plant ball 20 min, aseptic water washing 3 times with 10% tertiary sodium phosphate immersion again; Scale with tulip separates with the stem section then, selects the material of no scab, and with 70% alcohol disinfecting 30s, aseptic water washing 2 times is again with 5% liquor natrii hypochloritis, 10 min that sterilize, aseptic water washing 2 times; At last with 0.1% mercuric chloride solution, 10 min that sterilize, aseptic water washing 4 times;
(4) scale cutting: the tulip bulb of handling is at first removed scale top, account for 1/3 of whole bulb greatly, then the bulb of middle part and bottom vertically is cut into 16 strippings and slicings, more every stripping and slicing rip cutting is become to have the fritter of 3 scales and plateau;
(5) scale vernalization is handled: culture matrix (is comprised the peat composed of rotten mosses, garden mould, vermiculite, humus soil, four ratio is 1:2:1:2) utilize 1000 times of liquid of 75% tpn to carry out spray disinfectant, soup is advisable to go deep into matrix 1-2cm, and control matrix water content is 60%; Matrix is carried out embedding to scale section, and vernalization is 20 days under 25 ℃ and dark condition, during keep the matrix water content 60%, air humidity is controlled 70%, differentiates ramentum at the tulip scale;
(6) scale expands processing: smear 300mg/L GA at the ramentum that differentiation is come out
3Impel the differentiation of scale with the NAA of 100mg/L, after ramentum that differentiation is come out reached 90% or more, continuation was in temperature is 25 ℃ dark surrounds, carrying out scale expands and handled 30 days, keeping water supply in media content during this time is 70%, and air humidity is controlled 70%, forms the young ball of tulip gradually;
(7) young ball is transplanted: the young ball that will grow up to 15 min that sterilize in potassium permanganate 0.1 % soup transplant then, manage according to conventional method.
Claims (3)
1. a method of utilizing the tulip scale to breed tulip fast is characterized in that, may further comprise the steps:
(1) select that freshness height, vitality are strong, the big tulip seed balls of well-grown, growing way unanimity, utilize 1.2% potassium nitrate, 0.20% thiocarbamide, 600mg/L GA
3, 450 mg/L ethrel handle to remove the dormancy of tulip;
(2) refrigeration is handled: tulip seed balls is mixed being placed in the plastic sack with silt, fill with the liver moss after the sterilization around the bulb, humidity about 65% ties plastic sack then, and the dislocation refrigerator-freezer is handled 20 d down for 0 ℃;
(3) disinfect: will at first rinse well with clear water through the kind ball that refrigeration is handled, and plant ball 20 min, aseptic water washing 2-3 time with 10% tertiary sodium phosphate immersion again; Scale with tulip separates with the stem section then, selects the material of no scab, and with 70% alcohol disinfecting 30s, aseptic water washing 2-3 time is again with 5% liquor natrii hypochloritis, 10 min that sterilize, aseptic water washing 2-3 time; At last with 0.1% mercuric chloride solution, 10 min that sterilize, aseptic water washing 4-5 time;
(4) scale cutting: the tulip bulb of handling is at first removed scale top, account for the 1/4-1/3 of whole bulb greatly, then the bulb of middle part and bottom vertically is cut into 8 or 16 strippings and slicings, more every stripping and slicing rip cutting is become to have the fritter of 1-3 scale and plateau;
(5) scale vernalization is handled: with culture matrix after sterilization, control matrix water content is 50-60%, matrix is carried out embedding to the scale section, under 20-25 ℃ of dark condition vernalization 20-30 days, keep the matrix water content at 50-60% during this time, air humidity control differentiates ramentum at 60-70% at the tulip scale;
(6) scale expands processing: smear 200-300mg/L GA at the ramentum that differentiation is come out
3Impel the differentiation of scale with the NAA of 100-200 mg/L, after ramentum that differentiation is come out reaches more than 90%, continuation is in temperature is 20-25 ℃ dark surrounds, carrying out scale expands and handled 10-30 days, keeping water supply in media content during this time is 60-70%, air humidity control forms the young ball of tulip gradually at 60-70%;
(7) young ball is transplanted: the young ball that will grow up to 15 min that sterilize in potassium permanganate 0.1 % soup transplant then, manage according to conventional method.
2. the method for utilizing the tulip scale to breed tulip fast according to claim 1 is characterized in that, the culture matrix described in the step (5) comprises the peat composed of rotten mosses, garden mould, vermiculite, humus soil, and four ratio is 1:2:1:2.
3. the method for utilizing the tulip scale to breed tulip fast according to claim 1, it is characterized in that, matrix sterilization method described in the step (5) is to utilize 1 000 times of liquid of 75% tpn evenly to be sprayed on the culture matrix, is advisable to go deep into matrix 1-2cm.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103430848A (en) * | 2013-08-16 | 2013-12-11 | 苏州仁成生物科技有限公司 | Growing method for propagating tulip |
| CN104326819A (en) * | 2014-10-24 | 2015-02-04 | 柳州市天姿园艺有限公司 | Black tulip culture soil |
| CN104026021B (en) * | 2014-06-25 | 2016-04-20 | 陈巧兰 | Tulip tissue culture and rapid propagation method |
| CN107372123A (en) * | 2017-09-13 | 2017-11-24 | 界首市家丰家庭农场 | A kind of method for improving tulip flourish |
| CN108338034A (en) * | 2017-11-12 | 2018-07-31 | 柳州市天立农林科技有限公司 | A kind of cultural method of breeding Malus spectabilis |
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| CN102090249A (en) * | 2010-11-10 | 2011-06-15 | 天津滨海国际花卉科技园区股份有限公司 | Cuttage propagation method for bulb scale in tulip |
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- 2013-05-31 CN CN201310211702.XA patent/CN103238523B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102090249A (en) * | 2010-11-10 | 2011-06-15 | 天津滨海国际花卉科技园区股份有限公司 | Cuttage propagation method for bulb scale in tulip |
Non-Patent Citations (3)
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| TAKUJI OHYAMA，等: "Effect of cold storage treatment for forcing bulbs on the C and N metabolism of tulip plants", 《SOIL SCIENCE AND PLANT NUTRITION》 * |
| 周欢，等: "百合科植物组织培养的研究进展", 《湖北农业科学》 * |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103430848A (en) * | 2013-08-16 | 2013-12-11 | 苏州仁成生物科技有限公司 | Growing method for propagating tulip |
| CN104026021B (en) * | 2014-06-25 | 2016-04-20 | 陈巧兰 | Tulip tissue culture and rapid propagation method |
| CN104326819A (en) * | 2014-10-24 | 2015-02-04 | 柳州市天姿园艺有限公司 | Black tulip culture soil |
| CN107372123A (en) * | 2017-09-13 | 2017-11-24 | 界首市家丰家庭农场 | A kind of method for improving tulip flourish |
| CN107372123B (en) * | 2017-09-13 | 2019-06-11 | 界首市家丰家庭农场 | A method of improving tulip flourish |
| CN108338034A (en) * | 2017-11-12 | 2018-07-31 | 柳州市天立农林科技有限公司 | A kind of cultural method of breeding Malus spectabilis |
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