CN104026021A - Rapid propagation method of tulip tissue culture - Google Patents
Rapid propagation method of tulip tissue culture Download PDFInfo
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- CN104026021A CN104026021A CN201410288468.5A CN201410288468A CN104026021A CN 104026021 A CN104026021 A CN 104026021A CN 201410288468 A CN201410288468 A CN 201410288468A CN 104026021 A CN104026021 A CN 104026021A
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Abstract
The invention discloses a rapid propagation method of tulip tissue culture, and belongs to the technical field of plant tissue culture. The rapid propagation method comprises the following steps of selecting pedicels, with bud stems, of tulip plants, cutting into small pieces, disinfecting and inoculating to a bud-inducing culture medium; regulating the illumination intensity and the temperature, and culturing until green buds grow; then transferring to inoculate into a strong seedling culture medium, regulating the illumination intensity and the temperature, and culturing to obtain strong seedlings; transferring to inoculate to a rooting culture medium, regulating the illumination intensity and the temperature, and culturing to obtain tulip tissue culture seedlings; transplanting into a seedling-raising disk for refined seedling cultivation, controlling the air humidity to be 85% to 90%, regulating the illumination intensity, performing the seedling hardening cultivation, and transplanting to the open ground for cultivation at last. By using the rapid propagation method, the tulip propagation cycle is shortened.
Description
Technical field
The present invention relates to field of plant tissue culture technique, especially a kind of tulip tissue culture and rapid propagation method.
Background technology
Tulip, belongs to long-day flowers, property happiness on the sunny side, wind sheltering, suitable winter warm and moist, summer nice and cool dry weather.Tulip not only has higher ornamental value, and it is wet turbid to have treatment taste, chest distress, the contrary stomachache of vomitting, the effect of the greasy grade of halitosis tongue.Traditional modes of reproduction of tulip is bulb separation breeding, and its reproduction speed is slow, and new varieties are put the time that needs about 20 years on market, and the infecting of susceptible viral in cultivation process, causes its deterioration of variety serious.So, be badly in need of seeking a kind of high yield, high-quality, efficient propagation method.
Summary of the invention
The object of this invention is to provide a kind of tulip tissue culture and rapid propagation method, this tulip tissue culture and rapid propagation method can solve the slow problem of tulip reproduction speed.
In order to address the above problem, the technical solution used in the present invention is: a kind of tulip tissue culture and rapid propagation method, comprises the following steps:
A, choose the band bud scape of healthy and strong tulip plant, cut into the segment of 1 centimetre～1.5 centimetres, with 75% alcohol-pickled 30 seconds～60 seconds, aseptic water washing 3 times～5 times;
B, be seeded to bud inducing culture, regulating illumination 2200LUX～2500LUX, temperature is 23 ℃～26 ℃, cultivates 8 days～10 days to growing sprouting; Wherein, described bud inducing culture be take MS medium as minimal medium, adds 0.25 mg/litre～0.3 mg/litre methyl α-naphthyl acetate and 0.3 mg/litre～0.4 mg/litre 6-benzyl aminoadenine;
C, when described sprouting grows to 1 centimetre～2 centimetres, be inoculated in strong seedling culture base, regulating illumination 2300LUX～2500LUX, temperature is 23 ℃～25 ℃, cultivates 13 days～18 days to 1 centimetre～1.5 centimetres of heights of seedling; Wherein, described strong seedling culture base be take 1/2MS medium as minimal medium, adds 0.25 mg/litre～0.3 mg/litre methyl α-naphthyl acetate;
D, transferred into root media above-mentioned strong sprout, regulating illumination 2200 Lux～2500 Lux, temperature is 23 ℃～26 ℃, cultivates and to seedling base portion, grows the root of 1 centimetre～4 centimetres in 15 days～20 days, obtains tulip group training seedling; The described foster base of taking root be take 1/2MS medium as minimal medium, adds 0.25 mg/litre～0.3 mg/litre methyl α-naphthyl acetate and 0.1 mg/litre～0.2 mg/litre gibberellin;
When E, the root of training seedling until described tulip group grow to 0.5 centimetre～1 centimetre, tulip group is trained to transplantation of seedlings to vermiculite is housed, in the seedling-cultivating tray of the peat composed of rotten mosses and sawdust, carry out hardening cultivation, controlling air humidity is 85%～90%, and capable of regulating illumination intensity is 10000Lux～15000Lux; Wherein, vermiculite, the peat composed of rotten mosses, the mass ratio of sawdust is 1:2:2;
F, hardening are cultivated after 30 days～45 days, are transplanted to open country and cultivate.
Owing to having adopted technique scheme, the present invention compared with prior art has following beneficial effect:
The present invention be take the band bud scape of tulip plant and is organized cultivation as material, has greatly shortened the breeding cycle of tulip, and gained seedling early growth is healthy and strong, and is not subject to seasonal restrictions, can spread.
Embodiment
Below in conjunction with embodiment, the invention will be further described:
Embodiment 1
The band bud scape of choosing healthy and strong tulip plant, cuts into the segment of 1 centimetre, with 75% alcohol-pickled 30 seconds, and aseptic water washing 5 times; Described segment is seeded to bud inducing culture, and regulating illumination 2200LUX, adjusts the temperature to 23 ℃, cultivates 10 days to growing sprouting;
When described sprouting grows to 1 centimetre, be inoculated in strong seedling culture base, regulating illumination 2300 Lux, temperature is 23 ℃, cultivates 18 days to 1.5 centimetres of heights of seedling; Transferred into root media above-mentioned strong sprout, regulating illumination 2200 Lux, temperature is 23 ℃, cultivates and to seedling base portion, grows the root of 4 centimetres in 20 days, obtains tulip group training seedling; When the root of described tulip group training seedling grows to 0.5 centimetre, by tulip group training transplantation of seedlings, to vermiculite, the peat composed of rotten mosses, carries out hardening cultivation in the seedling-cultivating tray that the mass ratio of sawdust is 1:2:2, and controlling air humidity is 85%, and capable of regulating illumination intensity is 10000Lux; Hardening was cultivated after 30 days, was transplanted to open country and cultivated.
In the present embodiment, bud inducing culture is to take MS medium as minimal medium, adds 0.25 mg/litre methyl α-naphthyl acetate and 0.4 mg/litre 6-benzyl aminoadenine; Strong seedling culture base be take 1/2MS medium as minimal medium, adds 0.3 mg/litre methyl α-naphthyl acetate; The foster base of taking root be take 1/2MS medium as minimal medium, adds 0.25 mg/litre methyl α-naphthyl acetate and 0.1 mg/litre gibberellin.
Embodiment 2
The band bud scape of choosing healthy and strong tulip plant, cuts into the segment of 1.5 centimetres, with 75% alcohol-pickled 60 seconds, and aseptic water washing 3 times; Described segment is seeded to bud inducing culture, and regulating illumination 2500LUX, adjusts the temperature to 26 ℃, cultivates 8 days to growing sprouting; When described sprouting grows to 2 centimetres, be inoculated in strong seedling culture base, regulating illumination 2500 Lux, temperature is 25 ℃, cultivates 13 days to 1 centimetre of height of seedling; Transferred into root media above-mentioned strong sprout, regulating illumination 2500 Lux, temperature is 26 ℃, cultivates and to seedling base portion, grows the root of 1 centimetre in 15 days, obtains tulip group training seedling; When the root of described tulip group training seedling grows to 1 centimetre, by tulip group training transplantation of seedlings, to vermiculite, the peat composed of rotten mosses, carries out hardening cultivation in the seedling-cultivating tray that the mass ratio of sawdust is 1:2:2, and controlling air humidity is 90%, and capable of regulating illumination intensity is 15000Lux; Hardening was cultivated after 45 days, was transplanted to open country and cultivated.
In the present embodiment, bud inducing culture is to take MS medium as minimal medium, adds 0.3 mg/litre methyl α-naphthyl acetate and 0.3 mg/litre 6-benzyl aminoadenine; Strong seedling culture base be take 1/2MS medium as minimal medium, adds 0.25 mg/litre methyl α-naphthyl acetate; The foster base of taking root be take 1/2MS medium as minimal medium, adds 0.3 mg/litre methyl α-naphthyl acetate and 0.2 mg/litre gibberellin.
Embodiment 3
The band bud scape of choosing healthy and strong tulip plant, cuts into the segment of 1.5 centimetres, with 75% alcohol-pickled 45 seconds, and aseptic water washing 4 times; Described segment is seeded to bud inducing culture, and regulating illumination 2400LUX, adjusts the temperature to 25 ℃, cultivates 10 days to growing sprouting; When described sprouting grows to 2 centimetres, be inoculated in strong seedling culture base, regulating illumination 2300 Lux, temperature is 25 ℃, cultivates 15 days to 1 centimetre of height of seedling; Transferred into root media above-mentioned strong sprout, regulating illumination 2400 Lux, temperature is 25 ℃, cultivates and to seedling base portion, grows the root of 1 centimetre in 18 days, obtains tulip group training seedling; When the root of described tulip group training seedling grows to 1 centimetre, by tulip group training transplantation of seedlings, to vermiculite, the peat composed of rotten mosses, carries out hardening cultivation in the seedling-cultivating tray that the mass ratio of sawdust is 1:2:2, and controlling air humidity is 90%, and capable of regulating illumination intensity is 12000Lux; Hardening was cultivated after 40 days, was transplanted to open country and cultivated.
In the present embodiment, bud inducing culture is to take MS medium as minimal medium, adds 0.3 mg/litre methyl α-naphthyl acetate and 0.35 mg/litre 6-benzyl aminoadenine; Strong seedling culture base be take 1/2MS medium as minimal medium, adds 0.3 mg/litre methyl α-naphthyl acetate; The foster base of taking root be take 1/2MS medium as minimal medium, adds 0.3 mg/litre methyl α-naphthyl acetate and 0.15 mg/litre gibberellin.
Claims (1)
1. a tulip tissue culture and rapid propagation method, is characterized in that comprising the following steps:
A, choose the band bud scape of healthy and strong tulip plant, cut into the segment of 1 centimetre～1.5 centimetres, with 75% alcohol-pickled 30 seconds～60 seconds, aseptic water washing 3 times～5 times;
B, be seeded to bud inducing culture, regulating illumination 2200LUX～2500LUX, temperature is 23 ℃～26 ℃, cultivates 8 days～10 days to growing sprouting; Wherein, described bud inducing culture be take MS medium as minimal medium, adds 0.25 mg/litre～0.3 mg/litre methyl α-naphthyl acetate and 0.3 mg/litre～0.4 mg/litre 6-benzyl aminoadenine;
C, when described sprouting grows to 1 centimetre～2 centimetres, be inoculated in strong seedling culture base, regulating illumination 2300LUX～2500LUX, temperature is 23 ℃～25 ℃, cultivates 13 days～18 days to 1 centimetre～1.5 centimetres of heights of seedling; Wherein, described strong seedling culture base be take 1/2MS medium as minimal medium, adds 0.25 mg/litre～0.3 mg/litre methyl α-naphthyl acetate;
D, transferred into root media above-mentioned strong sprout, regulating illumination 2200 Lux～2500 Lux, temperature is 23 ℃～26 ℃, cultivates and to seedling base portion, grows the root of 1 centimetre～4 centimetres in 15 days～20 days, obtains tulip group training seedling; The described foster base of taking root be take 1/2MS medium as minimal medium, adds 0.25 mg/litre～0.3 mg/litre methyl α-naphthyl acetate and 0.1 mg/litre～0.2 mg/litre gibberellin;
When E, the root of training seedling until described tulip group grow to 0.5 centimetre～1 centimetre, tulip group is trained to transplantation of seedlings to vermiculite is housed, in the seedling-cultivating tray of the peat composed of rotten mosses and sawdust, carry out hardening cultivation, controlling air humidity is 85%～90%, and capable of regulating illumination intensity is 10000Lux～15000Lux; Wherein, vermiculite, the peat composed of rotten mosses, the mass ratio of sawdust is 1:2:2;
F, hardening are cultivated after 30 days～45 days, are transplanted to open country and cultivate.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105359874A (en) * | 2015-10-31 | 2016-03-02 | 余大春 | Pinelliacordata seedling culture medium and seedling culture method |
| CN107372123A (en) * | 2017-09-13 | 2017-11-24 | 界首市家丰家庭农场 | A kind of method for improving tulip flourish |
| CN115843682A (en) * | 2022-10-28 | 2023-03-28 | 华中农业大学 | Method for inducing and regenerating tulip embryonic axis callus |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101953300B (en) * | 2010-08-24 | 2012-04-25 | 浙江大学 | Tissue culture method for Curcuma wenyujin No.1 |
| CN102090331B (en) * | 2010-11-10 | 2013-02-20 | 天津滨海国际花卉科技园区股份有限公司 | Method for enhancing tulip tissue culture seedling |
| CN102907325B (en) * | 2012-11-06 | 2013-11-06 | 广东第二师范学院 | Method for utilizing culture technology to produce Solomon turmeric and red tulip seedlings |
| CN103238523B (en) * | 2013-05-31 | 2014-12-10 | 苏州瑞邦塑胶有限公司 | Method for rapidly breeding tulip by using tulip scales |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105359874A (en) * | 2015-10-31 | 2016-03-02 | 余大春 | Pinelliacordata seedling culture medium and seedling culture method |
| CN107372123A (en) * | 2017-09-13 | 2017-11-24 | 界首市家丰家庭农场 | A kind of method for improving tulip flourish |
| CN107372123B (en) * | 2017-09-13 | 2019-06-11 | 界首市家丰家庭农场 | A method of improving tulip flourish |
| CN115843682A (en) * | 2022-10-28 | 2023-03-28 | 华中农业大学 | Method for inducing and regenerating tulip embryonic axis callus |
| CN115843682B (en) * | 2022-10-28 | 2024-02-27 | 华中农业大学 | Method for inducing and regenerating tulip hypocotyl callus |
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Address after: Cheung Chau Fengshan County town of Hechi city the Guangxi Zhuang Autonomous Region 547600 Old Village Development Fund Association Patentee after: Chen Qiaolan Address before: 545007 the Guangxi Zhuang Autonomous Region District of Liuzhou city road two Xingbin District 24 Building 1 unit 302 room Patentee before: Chen Qiaolan |
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Effective date of registration: 20201119 Address after: 535000 3 / F, building 1, Qinzhou nixing pottery cultural and Creative Industrial Park (Millennium ancient pottery city), Qinzhou City, Guangxi Zhuang Autonomous Region Patentee after: Qinzhou Qinnan Jinwo Industrial Park Management Committee Address before: Cheung Chau Fengshan County town of Hechi city the Guangxi Zhuang Autonomous Region 547600 Old Village Development Fund Association Patentee before: Chen Qiaolan |