CN103404432A - Tissue culture and rapid propagation method of poinsettia - Google Patents
Tissue culture and rapid propagation method of poinsettia Download PDFInfo
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Abstract
The invention relates to a tissue culture and rapid propagation method of poinsettia, which belongs to the technical field of plant tissue culture. The tissue culture and rapid propagation method is characterized by comprising the steps of rapidly forming a large number of proliferation seedlings on a sterile culture medium by utilizing terminal buds or lateral buds of potted poinsettia plantlets, transplanting the seedlings into an outdoor small flowerpot after being induced to root, growing for 8-9 months and selling as a commodity, wherein the survival rate is high (above 89%). In the production of poinsettia, cuttage is mainly used for propagation, but as branches contain rich latex, the cuttage survival rate is low; the tissue culture and rapid propagation are high in difficulty of seedling rooting and low in rooting rate. Compared with conventional cuttage propagation, the tissue culture and rapid propagation method is high in efficiency, potted plants are good in shape, strong in growth vigor and high in commodity value, the rooting rate of tissue culture seedlings is high (reaching 80-85%, and overcoming the problem that the seedlings are difficult to take root), the culture cycle is appropriate (taking about 80 days from explant buds to rooted seedlings), the proliferation multiple is high (being 8-10 times), and the hormone combination of the culture medium is simple (being low in cost and convenient to promote).
Description
Technical field
The tissue that the present invention relates to a kind of ornamental flower poinsettia is cultivated and method for quickly breeding, belongs to biological technical field, specifically belongs to the Plant Tissue Breeding category.
Background technology
Poinsettia (
Euphorbia pulcherrima), also claim poinsettia, be the Euphorbiaceae euphorbia, evergreen or half evergreen shrubs, while blooming, leaf is vermilion, very beautiful, main for viewing and admiring.It originates in Central America Mexico one band, and all there is cultivation existing China various places.Its ornamental value and economic worth are all higher.
At New Year's Day, Spring Festival, most of flowers and trees also are in the dormant stage, and poinsettia is worn greenery, and the strain jacking is with crimson gorgeous petal-shaped bract, and the large look aquatic foods of shape are very noticeable, are the good flowers in red-letter day in winter, and it is " Christmas flower " so claim again.
Poinsettia is rare sets seeds or, substantially without the ability of setting seeds, by cuttage, breeds in production, but because branch contains abundant milk, cuttage survival rate is low, and some improved seeds branches are few in addition, introduces a fine variety initial stage breeding limited amount, is difficult to meet consumer demand.
At present, the tissue-culturing rapid propagation of domestic poinsettia research is few, not yet forms ripe cultivating system, especially on the taking root of seedling, also has certain difficulty.The present invention, by constantly groping and improveing, has obtained the tissue-culturing rapid propagation system of a set of maturation, for the large-scale production of poinsettia seedling provides technical reserve.
Summary of the invention
The object of the invention is to, provide a kind of cycle short, efficiency is high, and production cost is low, the method for energy scale Fast-propagation commodity poinsettia potted flower.
Technical scheme of the present invention, the steps include:
(1) explant sterilization is processed: choose without damage by disease and insect, robust growth, clean potted plant poinsettia plantlet; Use sterilizing scissors, tender terminal bud and 2～5 lateral buds (band blade) of clip plant top children, add a small amount of liquid detergent, rinses 3～5 min with running water; Put into aseptic beaker, on super-clean bench, first with sterile water, embathe 4～5 times (each 30～60S); Use again 75% alcohol-pickled 50～60S, tipping alcohol; Then use 0.1%HgCl
2Solution sterilization 8～10min, the tipping waste liquid; Finally use aseptic water washing 5～7 times, by material transfer to the sterile petri dish that is placed with blotting paper, suck dry moisture;
(2) inducing and first culture of aseptic seedling: by above-mentioned sterilization treatment, with the stem explants of terminal bud or lateral bud, be seeded in just culture base (MS+6-BA1.0～1.5mg/L+NAA 0.1～0.15mg/L+0.6% agar+3% sucrose, pH value is 5.8～6.0) on, cultivation temperature is 22～24 ℃, illuminance is 2000～2200Lx, and light application time is 12h/d; 24～27 days, the stem segment grew up to the seedling of high 2.5～4.3cm, seedling leaf dark green, robust growth;
(3) propagation of seedling and subculture are cultivated: by the above-mentioned just seedling of culture, be cut into 2～4 segments with bud, be seeded in subculture medium (MS+6-BA1.0～1.5mg/L+KT0.5～1.0mg/L+IAA0.4～0.6mg/L+0.6% agar+3% sucrose, pH value is 5.8～6.0) on, cultivation temperature is 22～24 ℃, illuminance is 2000～2200Lx, and light application time is 12h/d; 20～25 days, segment grew up to again high 3.3～5.0cm, the leaf look dark green seedling of growing thickly, and the propagation multiple is 8～10 times;
(4) culture of rootage of seedling: choose the seedling through subculture cultivation, growing way stalwartness, from base portion, intercept near the joint position, be linked into root media (1/2MS+IAA0.5～0.8mg/L+IBA 0.5～0.8mg/L+0.5% agar+2% sucrose, pH value is 5.8～6.0) in, cultivation temperature is 22～24 ℃, illuminance is 2000～2200Lx, and light application time is 12h/d; 18～23 days, the root of existing 4～6 the 1.0～2.5cm of seedling base portion generated, and rooting rate is 80～85%;
(5) transplanting of seedling: the seedling of taking root grows in bottle that cane is sturdy, the leaf look dark green, during high 4.5～6.0cm, can carry out the flowerpot transplanting, first bottle is removed to lid, places 2～3 days in culturing room, according to the growing state of seedling, keeps the skin wet at any time therebetween; Through KMnO
4The diameter of sterilization is in the small flower of 16cm, puts into the detritus soil through high-temperature sterilization, and the bottle seedling is moved in flowerpot, waters sufficient water, in culturing room, places 3～4 days, moves into greenhouse; Turned basin once in 2～3 months.
The invention has the beneficial effects as follows: utilize the potted plant poinsettia plant of 3-4 strain, by tissue culture technique, produce fast the commercial poinsettia plant of thousands of basins, its characteristics are as follows:
(1) efficiency is high, and the cycle is shorter, and the potted plant shapeliness, growing way is strong, commodity value is high, in Table 1;
Table 1 the inventive method and conventional method are relatively
| Method | Quantity | Characteristics |
| Tradition cuttage or division propagation | A but basin plant division 3-6 basin | From the branch that maternal plant is sheared, the base portion conduit is easily stopped up by milk, and Water Transportation is obstructed, and survival rate is low, the difficulty of taking root, plant growing way are poor, needs 10～12 months from the plant division to the commodity selling. |
| The tissue-culturing rapid propagation the present invention relates to | One basin can obtain several more than thousand ten thousand basins even | The group of having taken root training seedling because root system is many, root is sturdy, the ability that absorbs moisture and nutrition is more intense, growth is fast, plant forming, commodity value are high, need to 8～9 months to commodity selling from being transplanted into flowerpot. |
(2) high the present invention of short rooting rate of cycle of group training seedling is from plucking the explant bud, and to obtaining the seedling of taking root, whole process approximately needs about 80 days, and the cycle is shorter, and has overcome the problem that seedling is difficult to take root, and rooting rate is higher;
(3) in the low culture medium prescription of the present invention of production cost, used several conventional somatotropin (6-BA, KT, NAA, IAA, IBA), hormone combinations is simple, and cost is not high.
Embodiment
Following examples of implementation are to further illustrate of the present invention, are not limitations of the present invention.
Example one:
Choose without damage by disease and insect, robust growth, clean potted plant poinsettia plantlet; Use sterilizing scissors, tender terminal bud and 2 lateral buds (band blade) of clip plant top children, add a small amount of liquid detergent, rinses 5 min with running water; Put into aseptic beaker, on super-clean bench, first with sterile water, embathe (each 30S) 4 times; Use again 75% alcohol-pickled 60S, tipping alcohol; Then use 0.1%HgCl
2Solution sterilization 10min, the tipping waste liquid; Finally use aseptic water washing 7 times, by material transfer to the sterile petri dish that is placed with blotting paper, suck dry moisture;
By above-mentioned sterilization treatment, with the stem explants of terminal bud or lateral bud, be seeded in just culture base (MS+6-BA1.0mg/L+NAA 0.1mg/L+0.6% agar+3% sucrose, pH value is 5.8) on, cultivation temperature is 22 ℃, and illuminance is 2000Lx, and light application time is 12h/d; 25 days, the stem segment grew up to the seedling of high 3.0cm, seedling leaf dark green, robust growth;
By the above-mentioned just seedling of culture, be cut into 3 segments with bud, be seeded in subculture medium (MS+6-BA1.0mg/L+KT0.5mg/L+IAA0.5mg/L+0.6% agar+3% sucrose, pH value is 5.8) on, cultivation temperature is 22 ℃, and illuminance is 2000Lx, and light application time is 12h/d; 22 days, segment grew up to again high 4.0cm, the leaf look dark green seedling of growing thickly, and the propagation multiple is 9 times;
Choose the seedling through subculture cultivation, growing way stalwartness, from base portion, intercept near the joint position, be linked into root media (1/2MS+IAA0.5mg/L+IBA 0.5mg/L+0.5% agar+2% sucrose, pH value is 5.8) in, cultivation temperature is 22 ℃, illuminance is 2000Lx, and light application time is 12h/d; 20 days, the root of existing 6 the 1.0～2.5cm of seedling base portion generated, and rooting rate is 80%;
The seedling of taking root grows in bottle that cane is sturdy, the leaf look dark green, during high 4.5cm, can carry out the flowerpot transplanting, first bottle is removed to lid, places 2 days in culturing room, according to the growing state of seedling, keeps the skin wet at any time therebetween; Through KMnO
4The diameter of sterilization is in the small flower of 16cm, puts into the detritus soil through high-temperature sterilization, and the bottle seedling is moved in flowerpot, waters sufficient water, places 3 days in culturing room, moves into greenhouse; Turned basin in 2 months once.
Example two:
Choose without damage by disease and insect, robust growth, clean potted plant poinsettia plantlet; Use sterilizing scissors, tender terminal bud and 3 lateral buds (band blade) of clip plant top children, add a small amount of liquid detergent, rinses 3 min with running water; Put into aseptic beaker, on super-clean bench, first with sterile water, soak (each 60S) 5 times; Use again 75% alcohol-pickled 50S, tipping alcohol; Then use 0.1%HgCl
2Solution sterilization 9min, the tipping waste liquid; Finally use aseptic water washing 5 times, by material transfer to the sterile petri dish that is placed with blotting paper, suck dry moisture;
By above-mentioned sterilization treatment, with the stem explants of terminal bud or lateral bud, be seeded in just culture base (MS+6-BA1.5mg/L+NAA 0.15mg/L+0.6% agar+3% sucrose, pH value is 6.0) on, cultivation temperature is 24 ℃, and illuminance is 2200Lx, and light application time is 12h/d; 27 days, the stem segment grew up to the seedling of high 4.3cm, seedling leaf dark green, robust growth;
By the above-mentioned just seedling of culture, be cut into 4 segments with bud, be seeded in subculture medium (MS+6-BA1.5mg/L+KT1.0mg/L+IAA0.4mg/L+0.6% agar+3% sucrose, pH value is 6.0) on, cultivation temperature is 24 ℃, and illuminance is 2200Lx, and light application time is 12h/d; 25 days, segment grew up to again high 5.0cm, the leaf look dark green seedling of growing thickly, and the propagation multiple is 8 times;
Choose the seedling through subculture cultivation, growing way stalwartness, from base portion, intercept near the joint position, be linked into root media (1/2MS+IAA0.8mg/L+IBA 0.6mg/L+0.5% agar+2% sucrose, pH value is 6.0) in, cultivation temperature is 24 ℃, illuminance is 2200Lx, and light application time is 12h/d; 23 days, the root of existing 5 the 1.0～2.5cm of seedling base portion generated, and rooting rate is 85%;
The seedling of taking root grows in bottle that cane is sturdy, the leaf look dark green, during high 6.0cm, can carry out the flowerpot transplanting, first bottle is removed to lid, places 3 days in culturing room, according to the growing state of seedling, keeps the skin wet at any time therebetween; Through KMnO
4The diameter of sterilization is in the small flower of 16cm, puts into the detritus soil through high-temperature sterilization, and the bottle seedling is moved in flowerpot, waters sufficient water, places 4 days in culturing room, moves into greenhouse; Turned basin in 3 months once.
Example three:
Choose without damage by disease and insect, robust growth, clean potted plant poinsettia plantlet; Use sterilizing scissors, tender terminal bud and 5 lateral buds (band blade) of clip plant top children, add a small amount of liquid detergent, rinses 4 min with running water; Put into aseptic beaker, on super-clean bench, first with sterile water, soak (each 45S) 4 times; Use again 75% alcohol-pickled 55S, tipping alcohol; Then use 0.1%HgCl
2Solution sterilization 8min, the tipping waste liquid; Finally use aseptic water washing 6 times, by material transfer to the sterile petri dish that is placed with blotting paper, suck dry moisture;
By above-mentioned sterilization treatment, with the stem explants of terminal bud or lateral bud, be seeded in just culture base (MS+6-BA1.2mg/L+NAA 0.13mg/L+0.6% agar+3% sucrose, pH value is 5.8) on, cultivation temperature is 23 ℃, and illuminance is 2000Lx, and light application time is 12h/d; 24 days, the stem segment grew up to the seedling of high 2.5cm, seedling leaf dark green, robust growth;
By the above-mentioned just seedling of culture, be cut into 2 segments with bud, be seeded in subculture medium (MS+6-BA1.2mg/L+KT0.8mg/L+IAA0.6mg/L+0.6% agar+3% sucrose, pH value is 5.8) on, cultivation temperature is 23 ℃, and illuminance is 2000Lx, and light application time is 12h/d; 20 days, segment grew up to again high 3.3cm, the leaf look dark green seedling of growing thickly, and the propagation multiple is 10 times;
Choose the seedling through subculture cultivation, growing way stalwartness, from base portion, intercept near the joint position, be linked into root media (1/2MS+IAA0.6mg/L+IBA 0.8mg/L+0.5% agar+2% sucrose, pH value is 5.8) in, cultivation temperature is 23 ℃, illuminance is 2000Lx, and light application time is 12h/d; 18 days, the root of existing 4 the 1.0～2.5cm of seedling base portion generated, and rooting rate is 83%;
The transplanting of seedling: the seedling of taking root grows in bottle that cane is sturdy, the leaf look dark green, during high 5.5cm, can carry out the flowerpot transplanting, first bottle is removed to lid, places 3 days in culturing room, according to the growing state of seedling, keeps the skin wet at any time therebetween; Through KMnO
4The diameter of sterilization is in the small flower of 16cm, puts into the detritus soil through high-temperature sterilization, and the bottle seedling is moved in flowerpot, waters sufficient water, places 3 days in culturing room, moves into greenhouse; 2 first quarter moons turn basin once.
Example four:
Choose without damage by disease and insect, robust growth, clean potted plant poinsettia plantlet; Use sterilizing scissors, tender terminal bud and 3 lateral buds (band blade) of clip plant top children, add a small amount of liquid detergent, rinses 5 min with running water; Put into aseptic beaker, on super-clean bench, first with sterile water, soak (each 60S) 5 times; Use again 75% alcohol-pickled 60S, tipping alcohol; Then use 0.1%HgCl
2Solution sterilization 10min, the tipping waste liquid; Finally use aseptic water washing 6 times, by material transfer to the sterile petri dish that is placed with blotting paper, suck dry moisture;
By above-mentioned sterilization treatment, with the stem explants of terminal bud or lateral bud, be seeded in just culture base (MS+6-BA1.0mg/L+NAA 0.15mg/L+0.6% agar+3% sucrose, pH value is 5.8) on, cultivation temperature is 23 ℃, and illuminance is 2200Lx, and light application time is 12h/d; 26 days, the stem segment grew up to the seedling of high 3.8cm, seedling leaf dark green, robust growth;
By the above-mentioned just seedling of culture, be cut into 3 segments with bud, be seeded in subculture medium (MS+6-BA1.5mg/L+KT0.5mg/L+IAA0.5mg/L+0.6% agar+3% sucrose, pH value is 5.8) on, cultivation temperature is 23 ℃, and illuminance is 2200Lx, and light application time is 12h/d; 23 days, segment grew up to again high 4.5cm, the leaf look dark green seedling of growing thickly, and the propagation multiple is 9 times;
Choose the seedling through subculture cultivation, growing way stalwartness, from base portion, intercept near the joint position, be linked into root media (1/2MS+IAA0.7mg/L+IBA 0.7mg/L+0.5% agar+2% sucrose, pH value is 5.8) in, cultivation temperature is 23 ℃, illuminance is 2200Lx, and light application time is 12h/d; 22 days, the root of existing 6 the 1.0～2.5cm of seedling base portion generated, and rooting rate is 85%;
The transplanting of seedling: the seedling of taking root grows in bottle that cane is sturdy, the leaf look dark green, during high 5.0cm, can carry out the flowerpot transplanting, first bottle is removed to lid, places 3 days in culturing room, according to the growing state of seedling, keeps the skin wet at any time therebetween; Through KMnO
4The diameter of sterilization is in the small flower of 16cm, puts into the detritus soil through high-temperature sterilization, and the bottle seedling is moved in flowerpot, waters sufficient water, places 3 days in culturing room, moves into greenhouse; Turned basin in 3 months once.
Claims (2)
1. the tissue of a poinsettia is cultivated and method for quickly breeding, it is characterized in that, utilize terminal bud or the lateral bud of potted plant poinsettia plantlet, on aseptic culture medium, form rapidly a large amount of propagation seedlings, after the seedling root induction, be transplanted in outdoor small flower, survival rate high (more than 89%), grew 8～9 months, namely can be used as commodity selling, it comprises the following steps:
(1) explant sterilization is processed: choose without damage by disease and insect, robust growth, clean potted plant poinsettia plantlet; Use sterilizing scissors, tender terminal bud and 2～5 lateral buds (band blade) of clip plant top children, add a small amount of liquid detergent, rinses 3～5 min with running water; Put into aseptic beaker, on super-clean bench, first with sterile water, soak 4～5 times (each 30～60S); Use again 75% alcohol-pickled 50～60S, tipping alcohol; Then use 0.1%HgCl
2Solution sterilization 8～10min, the tipping waste liquid; Finally use aseptic water washing 5～7 times, by material transfer to the sterile petri dish that is placed with blotting paper, suck dry moisture;
(2) inducing and first culture of aseptic seedling: by above-mentioned sterilization treatment, with the stem explants of terminal bud or lateral bud, be seeded in just on the culture base, 24～27 days, the stem segment grew up to the seedling of high 2.5～4.3cm, seedling leaf dark green, robust growth;
(3) propagation of seedling and subculture are cultivated: will the above-mentioned just seedling of culture, be cut into 2～4 segments with bud, and be seeded on subculture medium, 20～25 days, segment grew up to again high 3.3～5.0cm, the leaf look dark green seedling of growing thickly, and breeding multiple is 8～10 times;
(4) culture of rootage of seedling: choose the seedling through subculture cultivation, growing way stalwartness,, near the intercepting of joint position, be linked in root media from base portion, 18～23 days, the root generation of existing 4～6 the 1.0～2.5cm of seedling base portion, rooting rate is 80～85%;
(5) transplanting of seedling: the seedling of taking root grows in bottle that cane is sturdy, the leaf look dark green, during high 4.5～6.0cm, can carry out the flowerpot transplanting, first bottle is removed to lid, places 2～3 days in culturing room, according to the growing state of seedling, keeps the skin wet at any time therebetween; Through KMnO
4The diameter of sterilization is in the small flower of 16cm, puts into the detritus soil through high-temperature sterilization, and the bottle seedling is moved in flowerpot, waters sufficient water, in culturing room, places 3～4 days, moves into greenhouse; Turned basin once in 2～3 months.
2. according to the poinsettia tissue of claim 1, cultivate and method for quickly breeding, the first culture base in (2) is: MS+6-benzylaminopurine (6-BA) 1.0～1.5mg/L+naa (NAA) 0.1～0.15mg/L+0.6% agar+3% sucrose; (3) subculture medium in is: MS+6-benzylaminopurine (6-BA) 1.0～1.5mg/L+kinetin (KT) 0.5～1.0mg/L+heteroauxin (IAA) 0.4～0.6mg/L+0.6% agar+3% sucrose; (4) root media in is: 1/2MS+IAA0.5～0.8mg/L+IBA 0.5～0.8mg/L+0.5% agar+2% sucrose; (2), the medium pH value of (3), (4) is 5.8～6.0, cultivation temperature is 22～24 ℃, illuminance is 2000～2200Lx, light application time is 12h/d.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107616090A (en) * | 2017-11-14 | 2018-01-23 | 佴辉 | Poinsettia breeding of new variety method and its scale rapid propagation method |
| CN108293879A (en) * | 2018-04-20 | 2018-07-20 | 西北大学 | A kind of rapid propagation method of the root of gansui |
| CN109618925A (en) * | 2018-11-07 | 2019-04-16 | 中南林业科技大学 | A method of suitable for a variety of shrubs and herbage flower micropropagation of plants |
| CN112715357A (en) * | 2020-12-17 | 2021-04-30 | 广州甘蔗糖业研究所湛江甘蔗研究中心 | Tissue culture rapid propagation method of damnacanthus davidii suitable for industrial production |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4634674A (en) * | 1983-03-25 | 1987-01-06 | Atlantic Richfield Company | Plant regeneration from protoplasts |
| CN102845312A (en) * | 2012-10-12 | 2013-01-02 | 江苏省农业科学院 | Method for obtaining poinsettia filial generation through utilizing young embryo rescue |
-
2013
- 2013-01-08 CN CN201310005394.5A patent/CN103404432B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4634674A (en) * | 1983-03-25 | 1987-01-06 | Atlantic Richfield Company | Plant regeneration from protoplasts |
| CN102845312A (en) * | 2012-10-12 | 2013-01-02 | 江苏省农业科学院 | Method for obtaining poinsettia filial generation through utilizing young embryo rescue |
Non-Patent Citations (2)
| Title |
|---|
| 曹帮华: "一品红组培快繁技术的初步研究", 《山东林业科技》 * |
| 陈璐: "一品红柠檬雪茎段组织培养快繁技术", 《江苏农业科学》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107616090A (en) * | 2017-11-14 | 2018-01-23 | 佴辉 | Poinsettia breeding of new variety method and its scale rapid propagation method |
| CN108293879A (en) * | 2018-04-20 | 2018-07-20 | 西北大学 | A kind of rapid propagation method of the root of gansui |
| CN108293879B (en) * | 2018-04-20 | 2021-06-01 | 西北大学 | Rapid propagation method of euphorbia kansui |
| CN109618925A (en) * | 2018-11-07 | 2019-04-16 | 中南林业科技大学 | A method of suitable for a variety of shrubs and herbage flower micropropagation of plants |
| CN109618925B (en) * | 2018-11-07 | 2021-10-01 | 中南林业科技大学 | Method suitable for rapid propagation of various shrubs and herbaceous flower plants |
| CN112715357A (en) * | 2020-12-17 | 2021-04-30 | 广州甘蔗糖业研究所湛江甘蔗研究中心 | Tissue culture rapid propagation method of damnacanthus davidii suitable for industrial production |
| CN112715357B (en) * | 2020-12-17 | 2022-01-04 | 广州甘蔗糖业研究所湛江甘蔗研究中心 | Tissue culture rapid propagation method of damnacanthus davidii suitable for industrial production |
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| CN103404432B (en) | 2016-01-13 |
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