CN103444529B - Method for reproduction and mass propagation of lower axis fragment plants of ormosia hosiei.et wils - Google Patents
Method for reproduction and mass propagation of lower axis fragment plants of ormosia hosiei.et wils Download PDFInfo
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Abstract
The invention discloses a method for reproduction and mass propagation of lower axis fragment plants of ormosia hosiei.et wils. The method comprises the steps of selecting and disinfecting a material, performing induced culture on lower axis buds, culturing cluster buds, culturing sound seedlings, performing root induction, finishing test-tube seedlings and transplanting bottle seedlings. The method solves the problems that in the prior art, seeds of ormosia hosiei.et wils are not easily available and hard, the skins of the seeds are poor in water permeability, the natural reproduction capability and the spreading and diffusion capability of the seeds are not strong, the fructification of the seeds is late, the natural reproduction is difficult, and good seedlings are scarce. The method is high in expanding propagation coefficient and short in propagation time, the culture process of seedlings of a rare native tree species, namely ormosia hosiei.et wils, is shortened, a lot of costs are saved, the survival rate of grown-up seedlings after transplantation is high, and the seedlings are robust, tall and straight, grow well and are in order and consistent. The method provides a technical support for development and utilization of plant seeds with high economic values.
Description
Technical field
The invention belongs to woody plant tissure culture technique field, be specifically related to a kind of ormosia hosiei lower shaft fragment plant regeneration and amount reproduction method.
Background technology
Ormosia hosiei (Ormosia hosiei.et Wils), another name: rosewood, Ormosia hosiei, black camphor tree, Wu Zhangsi, sarranine wood are Papilionaceae Papilionaceae.The peculiar autochthon of China, national secondary rare or endangered species.Ormosia hosiei timber is topmost wood raw material in current Chinese red wooden furniture.Ormosia hosiei integrates preciousness material, medicinal material value, landscape utilization, Forest Culture, the high economic worth of tool and exploitation prospect, and fruit ruby is mellow and full, glittering and translucent, does not stored for a long timely eat into immortal, is described as " plant ruby ".Its cultural deposits are deep, can be used to make garden " location, geomantic omen ", have in " the raw southern part of the country of red bean, the spring is sent out several, and hope monarch picks more, the most yearning between lovers of this thing ", so be called yearning between lovers red bean in Tang poetry.Half fallen leaves feature is conducive to people's sunshade in summer, the environmental protection and energy saving requirement of winter daylighting heat, tree performance is graceful, the dense the moon of tree crown covers ground, easily transplants, and Ormosia hosiei a kind of the stronger seeds of the lower contamination resistance of damage by disease and insect occurs, antipollution power in city is substantially suitable with ginkgo, cinnamomum japonicum, bearing tree can form separately a scape, is excellent Landscape Trees, does not have the record of specific damage by disease and insect.So the life-span of Ormosia hosiei is general all very long, therefore only otherwise artificial felling grows into ancient tree very easily naturally.
Ormosia hosiei has higher medical value, and be a kind of important Chinese herbal medicine, its root, stem, skin, Ye Junke are used as medicine.Seed can be used as medicine, and medicine name rde bean is commonly called as red bean, its taste: sweet, flat, sour, nontoxic; Cure mainly hernia, stomachache, detumescence, suppuration, strengthening the spleen and stomach, let out dysentery, defaecation, stasis, amenorrhoea, rheumathritis and nameless sores or boils etc.; Its root, leaf also can be used as medicine, cure mainly cruelly cold, cold in the stomach is inverse, abdominal pain due to acute vomiting and diarrhea, relieving alcoholism, is product in medicine.Because natural forest resource suffers a large amount of felling, cause existing natural resources to reduce in a large number, it is narrow that distribution says benefit, and bearing tree is day by day rare, an Ormosia hosiei kind group of mean people, mainly remaines in by gateway of the village and temple with Feng Shui Woodland, international market is regarded as treasure in wood.Ormosia hosiei material is excellent, solid corrosion resistant, and contractility is little, its beautiful texture, can, with mahogany than beautiful, be the commerical tree species of China's preciousness.For a long time due to masses' denudation, natural forest resource is on the brink of exhaustion, is badly in need of development artificial afforestration, expands population scale.Because the ormosia hosiei solid age is slow; and have dividing of biennial bearing; generally just blossomed and beared fruit at about 35 years; result contained the phase after 50 years; and usually need interval to yield positive results for 3-5 years; carry out the selection of fine individual plant and take tissue cultures vegetative propagation technique to carry out large-scale production; for cultivating the protective development of high quality seedling and precious indigenous tree species; adjustment forestry practices structure; promote forestry sustainable development, to efficient child care Precious, Rare, Endangered Ormosia hosiei resource, there is important Research Significance and higher economic worth and exploitation prospect.
Summary of the invention
The object of the invention is to provide a kind of method of ormosia hosiei lower shaft fragment plant regeneration, amount reproduction, large-scale production, solves Ormosia hosiei seed source difficulty in prior art, subjects to mouse food; Seed is hard, is not easy to absorb moisture after seed coat drying; Seed coat bad hydraulic permeability, could must sprout through special vernalization process, natural renovation difficulty, natural procreation ability and Spreading and diffusion indifferent; The solid age late and need interval to yield positive results for 3 ~ 5 years, the problem that good seed is rare.
Method of the present invention realizes as follows:
A kind of ormosia hosiei lower shaft fragment plant regeneration and amount reproduction method, it is characterized in that comprising material choose with sterilize, lower shaft bud inducement is cultivated, adventitious shoots culture, strong seedling culture, root induction, test-tube plantlet complete, bottle transplantation of seedlings.Concrete steps are as follows:
1) material selection and sterilization: selected seed aseptically plumule attenuating the healthy and strong seedling of unrooted growing 2 ~ 3 leaves cuts epicotyl and lower shaft fragment after breeding is culture materials, adopts exogenous hormone process culture materials;
2) lower shaft bud inducement is cultivated: get the lower shaft fragment after process and be inoculated in the lower shaft bud inducement medium of sterilizing, 1/3 cotyledon part is cut off in cotyledon position, temperature 23 ± 2 DEG C, light application time 14h/d, luminous intensity is 1000 ~ 1500lx, and lower shaft is cultivated 15 ~ 25 days;
3) adventitious shoots culture: lower shaft bud inducement is cultivated the well-grown Multiple Buds obtained and be cut into the long band liquid leaf stem section of 1 ~ 2cm, be seeded in adventitious shoots culture base and carry out inducing clumping bud cultivation, culturing room's temperature is 23 ± 2 DEG C, light application time 14h/d, intensity of illumination is 1500 ~ 2000lx, and clump bud inducement is cultivated 30 ~ 50 days;
4) strong seedling culture: cut cultivating through clump bud inducement the well-grown whole plant individual plant obtained, with 1 ~ 2 leaf, plant height 1 ~ 1.5cm, transfer in strong seedling culture base, culturing room's temperature is 23 ± 2 DEG C, light application time 14h/d, intensity of illumination is 1500 ~ 2000lx, strong seedling culture 25 ~ 35 days is visible in plant base portion circumferential surface graininess afterwards, and the irregular band green calli of profile, contributes to plant like this and take root;
5) root induction: by strong seedling culture seedling individual plant out, with 2 ~ 3 leaves, plant height 1 ~ 2cm, be transferred directly in root media, culturing room's temperature is 23 ± 2 DEG C, light application time 14h/d, intensity of illumination is 1500 ~ 2000lx, culture of rootage 30 ~ 40 days;
6) test-tube plantlet completes: test-tube plantlet growth to the 3 ~ 4cm turned out when root induction is high, has 3 ~ 5 roots, 3 ~ 6 blades, during leaf length 2 ~ 3cm, completes Ormosia hosiei lower shaft plant regeneration and cultivates and the cultivation of plant amount reproduction test-tube plantlet;
7) bottle transplantation of seedlings: the test-tube plantlet completed is placed on natural daylight lower refining seedling 3 ~ 5 days, then open bottle cap and carry out hardening 2 days; Then take out from blake bottle, wash away the medium being attached to root system, move in the matrix that vermiculite and humus soil mix with mass ratio 1:2, be placed on temperature 15 ~ 30 DEG C, humidity, 75% ~ 85%, is cultivated under avoiding the environment of direct sunlight, the healthy and strong complete seedling of ormosia hosiei of acquisition.
Wherein, described step 2) lower shaft bud inducement culture medium prescription is WPM+BA 1.5mgL
-1 +iAA 0.5mgL
-1+ NAA 0.3mgL
-1+ TDZ 0.01mgL
-1+ Ag 6.0gL
-1+ Su 20gL
-1+ active carbon 1.0 ~ 1.5 gL
-1, medium thickness is 1.4 ~ 1.6cm.
The formula of described step 3) inducing clumping bud medium is WPM+BA 1.5mgL
-1 +iAA1.0mgL
-1 +tDZ 0.02mgL
-1 +nAA 0.5mgL
-1+ Ag 6.0gL
-1+ Su 20gL
-1+ active carbon 1.0 ~ 1.5 gL
-1, medium thickness is 1.4 ~ 1.6cm.
The formula of described step 4) strong seedling culture base is WPM+BA 1.5mgL
-1 +kT 1.0mgL
-1 +tDZ 0.02mgL
-1 +iAA 0.5mgL
-1 +nAA 0.5mgL
-1+ Ag 6.0gL
-1+ Su 30gL
-1+ active carbon 1.5 ~ 2.0 gL
-1, medium thickness is 1.4 ~ 1.6cm.
The formula of described step 5) root media is 1/2 WPM+NAA 0.3mgL
-1+ IBA 1.0mgL
-1+ root sun 0.25mlL
-1+ Ag 6.0gL
-1+ Su 30gL
-1+ active carbon 1.5 ~ 2.0gL
-1, medium thickness is 1.4 ~ 1.6cm.
Remarkable advantage of the present invention is: take the lead in adopting ormosia hosiei lower shaft fragment Fiber differentiation to obtain complete and a large amount of plant, by lower shaft evoking adventive bud, achieve the repeatability of ECIWO, be the interactional result of growing environment of medium combination, utilize Holographic Phenomenon and theoretical explant pedestrian's work of going forward side by side of choosing to cultivate and to preserve for rare plant resource high-efficiency and development rare tree has important research and realistic meaning.Within 15 ~ 25 days, healthy and strong plumule can be gone out by germination and growth; Again through 30 ~ 50 days Multiplying culture and 25 ~ 35 days strong seedling culture, after root induction in 30 ~ 40 days, get final product seedling, transplanting, survival rate is up to more than 97%; Expand numerous coefficient more than 18 times, this cultural method expands that numerous coefficient is higher, and generation time is short, seedling transplant after survival rate high, seedling growth stalwartness is tall and straight, and blade BG, grows fine, for the exploitation of high economic worth seeds provide technical support.
Accompanying drawing explanation
Cotyledon after Fig. 1 Ormosia hosiei lower shaft fragment cuts off 1/3
Fig. 2 Ormosia hosiei lower shaft bud inducement
Fig. 3 lower shaft inducing clumping bud
Fig. 4 Ormosia hosiei lower shaft strong seedling culture
Fig. 5 Ormosia hosiei lower shaft forms the plant of root system
The red bean sapling of Fig. 6 transplant survival
Embodiment
In order to fully disclose ormosia hosiei lower shaft fragment plant regeneration of the present invention and amount reproduction method, the present invention is described further below, but the present invention is not limited thereto.
embodiment 1
1) material selection and sterilization: selected seed aseptically plumule attenuating the healthy and strong seedling of unrooted growing 3 leaves cuts epicotyl and lower shaft fragment after breeding is culture materials, adopts exogenous hormone process culture materials.
2) lower shaft bud inducement is cultivated: get the lower shaft fragment after process and be inoculated in the lower shaft bud inducement medium of sterilizing, culture medium prescription is WPM+BA 1.5mgL
-1 +iAA 0.5mgL
-1+ NAA 0.3mgL
-1+ TDZ 0.01mgL
-1+ Ag 6.0gL
-1+ Su 20gL
-1+ active carbon 1.5 gL
-1, medium thickness is 1.5cm.Cut off 1/3 cotyledon part (see figure 1) in cotyledon position, temperature 23 DEG C, light application time 14h/d, luminous intensity is 1500lx, and lower shaft 20 days, is shown in Fig. 2.
3) adventitious shoots culture: lower shaft bud inducement is cultivated the well-grown Multiple Buds obtained and be cut into the long band liquid leaf stem section of 2cm, be seeded in adventitious shoots culture base and carry out inducing clumping bud cultivation.The formula of medium is WPM+BA 1.5mgL
-1 +iAA1.0mgL
-1 +tDZ 0.02mgL
-1 +nAA 0.5mgL
-1+ Ag 6.0gL
-1+ Su 20gL
-1+ active carbon 1.5 gL
-1, medium thickness is 1.5cm.Culturing room's temperature is 23 DEG C, light application time 14h/d, and intensity of illumination is 2000lx, and clump bud lures cultivation 45 days, sees Fig. 3.
4) strong seedling culture: cut by the well-grown whole plant individual plant luring cultivation to obtain through clump bud, with 2 leaves, plant height 1.5cm, transfers in strong seedling culture base.The formula of medium is WPM+BA 1.5mgL
-1 +kT 1.0mgL
-1 +tDZ 0.02mgL
-1 +iAA 0.5mgL
-1 +nAA 0.5mgL
-1+ Ag 6.0gL
-1+ Su 30gL
-1+ active carbon 2.0 gL
-1, medium thickness is 1.5cm.Culturing room's temperature is 23 DEG C, light application time 14h/d, and intensity of illumination is 2000lx.
Strong seedling culture 28 days, as shown in Figure 4, as seen in plant base portion circumferential surface graininess, the irregular band green calli of profile, contributes to plant like this and takes root.
5) root induction: by strong seedling culture seedling individual plant out, be with 2 leaves, plant height 2cm, is transferred directly in root media.The formula of medium is 1/2 WPM+NAA 0.3mgL
-1+ IBA 1.0mgL
-1+ root the sun 0.25 mlL
-1+ Ag 6.0gL
-1+ Su 30gL
-1+ active carbon 2.0gL
-1, medium thickness is 1.5cm.Culturing room's temperature is 23 DEG C, light application time 14h/d, and intensity of illumination is 2000lx, culture of rootage 35 days.
6) test-tube plantlet completes: the test-tube plantlet growth of turning out when root induction is high to 3cm, has 3 roots, 4 blades, during the long 2.5cm of leaf, completes Ormosia hosiei lower shaft plant regeneration and to cultivate and plant amount reproduction test-tube plantlet cultivates (see figure 5).
7) bottle transplantation of seedlings: the test-tube plantlet completed to be placed under natural daylight refining 5 days, then open bottle cap and carry out hardening 2 days; Then take out from blake bottle, wash away the medium being attached to root system, move in the matrix that vermiculite and humus soil mix with mass ratio 1:2, be placed on temperature 25 DEG C, humidity, 75%, is cultivated under avoiding the environment of direct sunlight, the healthy and strong complete seedling (see figure 6) of ormosia hosiei of acquisition.Plant shoot survival percent up to more than 97%, expand numerous coefficient more than 18 times.
embodiment 2
1) material selection and sterilization: selected seed aseptically plumule attenuating the healthy and strong seedling of unrooted growing 3 leaves cuts epicotyl and lower shaft fragment after breeding is culture materials, adopts exogenous hormone process culture materials.
2) lower shaft bud inducement is cultivated: get the lower shaft fragment after process and be inoculated in the lower shaft bud inducement medium of sterilizing, culture medium prescription is WPM+BA 1.5mgL
-1 +iAA0.5mgL
-1+ NAA 0.3mgL
-1+ TDZ 0.01mgL
-1+ Ag 6.0gL
-1+ Su 20gL
-1+ active carbon 1.2 gL
-1, medium thickness is 1.4cm.Cut off 1/3 cotyledon part in cotyledon position, temperature 21 DEG C, light application time 14h/d, luminous intensity is 1300lx, and lower shaft cultivates 15 days.
3) adventitious shoots culture: lower shaft bud inducement is cultivated the well-grown Multiple Buds obtained and be cut into the long band liquid leaf stem section of 1.5cm, be seeded in adventitious shoots culture base and carry out inducing clumping bud cultivation, the formula of medium is WPM+BA 1.5mgL
-1 +iAA1.0mgL
-1 +tDZ 0.02mgL
-1 +nAA 0.5mgL
-1+ Ag 6.0gL
-1+ Su 20gL
-1+ active carbon 1.2 gL
-1, medium thickness is 1.4cm.Culturing room's temperature is 21 DEG C, light application time 14h/d, and intensity of illumination is 1800lx, and clump bud inducement cultivates 30 days.
4) strong seedling culture: cut cultivating through clump bud inducement the well-grown whole plant individual plant obtained, with 2 leaves, plant height 1cm, transfers in strong seedling culture base, and the formula of medium is WPM+BA 1.5mgL
-1 +kT 1.0mgL
-1 +tDZ 0.02mgL
-1 +iAA 0.5mgL
-1 +nAA 0.5mgL
-1+ Ag 6.0gL
-1+ Su 30gL
-1+ active carbon 1.8 gL
-1, medium thickness is 1.4cm.Culturing room's temperature is 21 DEG C, light application time 14h/d, and intensity of illumination is 1800lx, strong seedling culture 25 days.
5) root induction: by strong seedling culture seedling individual plant out, with 2 leaves, plant height 1cm, is transferred directly in root media, the formula of medium is 1/2 WPM+NAA 0.3mgL
-1+ IBA 1.0mgL
-1+ root the sun 0.25 mlL
-1+ Ag 6.0gL
-1+ Su 30gL
-1+ active carbon 1.8gL
-1, medium thickness is 1.4cm.Culturing room's temperature is 21 DEG C, light application time 14h/d, and intensity of illumination is 1800lx, culture of rootage 30 days.
6) test-tube plantlet completes: the test-tube plantlet growth of turning out when root induction is high to 3.5cm, has 4 roots, 6 blades, during the long 3cm of leaf, completes Ormosia hosiei lower shaft plant regeneration and to cultivate and plant amount reproduction test-tube plantlet is cultivated.
7) bottle transplantation of seedlings: the test-tube plantlet completed is placed on natural daylight lower refining seedling 4 days, then open bottle cap and carry out hardening 2 days; Then take out from blake bottle, wash away the medium being attached to root system, move in the matrix that vermiculite and humus soil mix with mass ratio 1:2, be placed on temperature 15 DEG C, humidity, 85%, is cultivated under avoiding the environment of direct sunlight, the healthy and strong complete seedling of ormosia hosiei of acquisition.
embodiment 3
1) material selection and sterilization: selected seed aseptically plumule attenuating the healthy and strong seedling of unrooted growing 2 leaves cuts epicotyl and lower shaft fragment after breeding is culture materials, adopts exogenous hormone process culture materials.
2) lower shaft bud inducement is cultivated: get the lower shaft fragment after process and be inoculated in the lower shaft bud inducement medium of sterilizing, culture medium prescription is WPM+BA 1.5mgL
-1 +iAA 0.5mgL
-1+ NAA 0.3mgL
-1+ TDZ 0.01mgL
-1+ Ag 6.0gL
-1+ Su 20gL
-1+ active carbon 1.0 gL
-1, medium thickness is 1.6cm.Cut off 1/3 cotyledon part in cotyledon position, temperature 25 DEG C, light application time 14h/d, luminous intensity is 1000lx, and lower shaft cultivates 25 days.
3) adventitious shoots culture: lower shaft bud inducement is cultivated the well-grown Multiple Buds obtained and be cut into the long band liquid leaf stem section of 1cm, be seeded in adventitious shoots culture base and carry out inducing clumping bud cultivation, the formula of medium is WPM+BA 1.5mgL
-1 +iAA1.0mgL
-1 +tDZ 0.02mgL
-1 +nAA 0.5mgL
-1+ Ag 6.0gL
-1+ Su 20gL
-1+ active carbon 1.0 gL
-1, medium thickness is 1.6cm.Culturing room's temperature is 25 DEG C, light application time 14h/d, and intensity of illumination is 1500lx, and clump bud lures cultivation 50 days.
4) strong seedling culture: cut by the well-grown whole plant individual plant luring cultivation to obtain through clump bud, with 1 leaf, plant height 1.5cm, transfers in strong seedling culture base, and the formula of medium is WPM+BA 1.5mgL
-1 +kT 1.0mgL
-1 +tDZ 0.02mgL
-1 +iAA 0.5mgL
-1 +nAA 0.5mgL
-1+ Ag 6.0gL
-1+ Su 30gL
-1+ active carbon 1.5 gL
-1, medium thickness is 1.6cm.Culturing room's temperature is 25 DEG C, light application time 14h/d, and intensity of illumination is 1500lx, strong seedling culture 35 days.
5) root induction: by strong seedling culture seedling individual plant out, with 3 leaves, plant height 1.5cm, is transferred directly in root media, the formula of medium is 1/2 WPM+NAA 0.3mgL
-1+ IBA 1.0mgL
-1+ root the sun 0.25 mlL
-1+ Ag 6.0gL
-1+ Su 30gL
-1+ active carbon 1.5gL
-1, medium thickness is 1.6cm.Culturing room's temperature is 25 DEG C, light application time 14h/d, and intensity of illumination is 1500lx, culture of rootage 40 days.
6) test-tube plantlet completes: the test-tube plantlet growth of turning out when root induction is high to 4cm, has 5 roots, 3 blades, during the long 2cm of leaf, completes Ormosia hosiei lower shaft plant regeneration and to cultivate and plant amount reproduction test-tube plantlet is cultivated.
7) bottle transplantation of seedlings: the test-tube plantlet completed is placed on natural daylight lower refining seedling 3 days, then open bottle cap and carry out hardening 2 days; Then take out from blake bottle, wash away the medium being attached to root system, move in the matrix that vermiculite and humus soil mix with mass ratio 1:2, be placed on temperature 30 DEG C, humidity, 80%, is cultivated under avoiding the environment of direct sunlight, the healthy and strong complete seedling of ormosia hosiei of acquisition.
Claims (1)
1. an ormosia hosiei plant regeneration and amount reproduction method, it is characterized in that: comprise material choose with sterilize, lower shaft bud inducement is cultivated, inducing clumping bud is cultivated, strong seedling culture, root induction are cultivated, complete that test-tube plantlet is cultivated, bottle transplantation of seedlings, concrete steps are as follows:
1) material selection and sterilization: selected seed aseptically plumule attenuating the healthy and strong seedling of unrooted growing 2 ~ 3 leaves cuts epicotyl and lower shaft fragment after breeding is culture materials, adopts exogenous hormone process culture materials;
2) lower shaft bud inducement is cultivated: get the lower shaft fragment after process and be inoculated in the lower shaft bud inducement medium of sterilizing, 1/3 cotyledon part is cut off in cotyledon position, temperature 23 ± 2 DEG C, light application time 14h/d, luminous intensity is 1000 ~ 1500lx, and lower shaft is cultivated 15 ~ 25 days; Wherein, lower shaft bud inducement culture medium prescription is WPM+BA 1.5mgL
-1+ IAA 0.5mgL
-1+ NAA 0.3mgL
-1+ TDZ 0.01mgL
-1+ agar 6.0gL
-1+ sucrose 20gL
-1+ active carbon 1.0 ~ 1.5 gL
-1, medium thickness is 1.4 ~ 1.6cm;
3) inducing clumping bud is cultivated: lower shaft bud inducement is cultivated the well-grown Multiple Buds obtained and be cut into the long stem segment with axillary bud of 1 ~ 2cm, be seeded in inducing clumping bud medium and carry out inducing clumping bud cultivation, culturing room's temperature is 23 ± 2 DEG C, light application time 14h/d, intensity of illumination is 1500 ~ 2000lx, and inducing clumping bud is cultivated 30 ~ 50 days; Wherein, the formula of inducing clumping bud medium is WPM+BA 1.5mgL
-1+ IAA 1.0mgL
-1+ TDZ 0.02mgL
-1+ NAA 0.5mgL
-1+ agar 6.0gL
-1+ sucrose 20gL
-1+ active carbon 1.0 ~ 1.5 gL
-1, medium thickness is 1.4 ~ 1.6cm;
4) strong seedling culture: cut cultivating obtained well-grown whole plant individual plant through inducing clumping bud, with 1 ~ 2 leaf, plant height 1 ~ 1.5cm, transfer in strong seedling culture base, culturing room's temperature is 23 ± 2 DEG C, light application time 14h/d, intensity of illumination is 1500 ~ 2000lx, strong seedling culture 25 ~ 35 days; Wherein, strong seedling culture based formulas is WPM+BA 1.5mgL
-1+ KT 1.0mgL
-1+ TDZ 0.02mgL
-1+ IAA 0.5mgL
-1+ NAA 0.5mgL
-1+ agar 6.0gL
-1+ sucrose 30gL
-1+ active carbon 1.5 ~ 2.0 gL
-1, medium thickness is 1.4 ~ 1.6cm;
5) root induction is cultivated: by strong seedling culture seedling individual plant out, with 2 ~ 3 leaves, plant height 1 ~ 2cm, be transferred directly in root media and cultivate, culturing room's temperature is 23 ± 2 DEG C, light application time 14h/d, intensity of illumination is 1500 ~ 2000lx, culture of rootage 30 ~ 40 days; Wherein, prescription of rooting medium is 1/2 WPM+NAA 0.3mgL
-1+ IBA 1.0mgL
-1+ root sun 0.25mlL
-1+ agar 6.0gL
-1+ sucrose 30gL
-1+ active carbon 1.5 ~ 2.0gL
-1, medium thickness is 1.4 ~ 1.6cm;
6) complete test-tube plantlet to cultivate: test-tube plantlet growth to the 3 ~ 4cm turned out when root induction is high, has 3 ~ 5 roots, 3 ~ 6 blades, during leaf length 2 ~ 3cm, complete Ormosia hosiei lower shaft plant regeneration and cultivate;
7) bottle transplantation of seedlings: the bottle seedling completing test-tube plantlet cultivation is placed on natural daylight lower refining seedling 3 ~ 5 days, then opens bottle cap hardening 2 days; Then from blake bottle, plant is taken out, wash away the medium be attached on root system, move in vermiculite and humus soil mass ratio 1: 2 mixed-matrix, be placed on temperature 15 ~ 30 DEG C, humidity 75% ~ 85%, cultivate under avoiding the environment of direct sunlight, thus obtain the healthy and strong complete seedling of ormosia hosiei.
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| CN104541671A (en) * | 2014-12-02 | 2015-04-29 | 福建农林大学 | Method for accelerating germination of ormosia hosiei seeds |
| CN104663195A (en) * | 2015-01-29 | 2015-06-03 | 江苏绿峰景观工程有限公司 | Method of raising ormosia seedlings by cutting |
| CN109220805A (en) * | 2018-11-05 | 2019-01-18 | 贵州大学 | A kind of Ormosia hosiei tissue culture outside sprout-cultivating-bottle radication method |
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|---|---|---|---|---|
| CN1695429A (en) * | 2005-06-23 | 2005-11-16 | 中国科学院武汉植物园 | Quick method for breeding rosewood |
| CN102511390A (en) * | 2011-11-28 | 2012-06-27 | 云南云科花卉有限公司 | Method and special culture medium for regenerating sterile induced plants of ormosia fordiana seeds |
| CN102657088A (en) * | 2012-05-11 | 2012-09-12 | 中国林业科学研究院亚热带林业研究所 | Tissue culture method for Ormosia hosiei et Wils |
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