CN109220805A - A kind of Ormosia hosiei tissue culture outside sprout-cultivating-bottle radication method - Google Patents
A kind of Ormosia hosiei tissue culture outside sprout-cultivating-bottle radication method Download PDFInfo
- Publication number
- CN109220805A CN109220805A CN201811309483.8A CN201811309483A CN109220805A CN 109220805 A CN109220805 A CN 109220805A CN 201811309483 A CN201811309483 A CN 201811309483A CN 109220805 A CN109220805 A CN 109220805A
- Authority
- CN
- China
- Prior art keywords
- cultivating
- culture
- bottle
- seedling
- ormosia hosiei
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of Ormosia hosiei tissue culture outside sprout-cultivating-bottle radication methods, method includes the following steps: selecting seedling stem section as explant, it carries out disinfection to be placed in induced medium after disinfecting action and carries out inducing clumping bud, make explant with the leaf stem section of growing thickly of induction and carries out Multiplying culture, healthy and strong bottle seedling by Multiplying culture higher than 3 ~ 5cm moves under natural light after 3 ~ 4d, ventilated membrane is opened in 3 ~ 4d of room temperature lower refining seedling, proliferation clump bud is cut into single bud again, base portion is in 4 ~ 5s of NAA100 ~ 200mg/L middling speed libation at an ancient wedding ceremony, cuttage carries out outside sprout-cultivating-bottle culture into the matrix of sterilization, rooting rate is up to 97.78%.The present invention is combined the rooting process of tissue-cultured seedling and hardening domestication using the method for outside sprout-cultivating-bottle, is improved the quality and survival rate of tissue-cultured seedling, is simplified tissue culture production link, is shortened the Ormosia hosiei emergence period, is reduced and cultivate cost, is conducive to push industrial seedling rearing.
Description
Technical field
The present invention relates to a kind of Ormosia hosiei tissue culture outside sprout-cultivating-bottle radication methods, belong to the cultivating and growing technical field of Ormosia hosiei.
Background technique
Ormosia hosieiOrmosia hosieiHemsl. et Wils. is Papilionaceae Ormosia aiphyllium, is that China is special
There is indigenous tree, is born in the hills, river bank or mountain valley evergreen broadleaf forest of 400~650m of height above sea level more.Its heartwood is hard, deep brown
Color, sapwood is khaki, and it is the preferred material of foremost Longquan sword scabbard and pommel that texture is unique；Its tree-like grace, leaf
Color brilliant green, autumn pod are spat red, are good afforestation ornamental tree species；Ormosia hosiei also has a preferable medical value, root,
Stem, skin, leaf can be used as medicine, and cure mainly the illnesss such as traumatic injury, rheumathritis and nameless sores or boils.Ormosia hosiei be the precious material of collection,
Medicinal, afforestation, Forest Culture are in the indigenous tree of one.Ormosia hosiei blossom and bear fruit rule it is unstable, it is solid evening and seed
Biennial bearing phenomenon especially severe just blossoms and bears fruit for the general age of tree 35 years or so, and 5~10a result is primary or even decades do not tie
Seed.Its resource is in Critical Condition at present, and special wild red Caragana arborescens resource tends to be exhausted, has been cited as II grade of emphasis of country and protects
Protect rare and endangered wild plant.With the continuous improvement of people's quality of the life, the demand of high quality timber is also increasingly increased, closely
The Ormosia hosiei market demand is big over year, and supply falls short of demand.Forefathers' research shows that Ormosia hosiei cuttage, propagation by grafiting to there is survival rate low
The phenomenon that, existing document " Ormosia hosiei tissue culture technique (Fan Huihua, the such as Li Zhaohui Fujian Forestry science and technology, September in 2011 the
3 phases volume 38) " in inductivity it is lower, only 52.05%, application No. is 201210144494.1 Chinese inventions on this basis
Patent application discloses a kind of Ormosia hosiei method for tissue culture, by the way of taking root in bottle, although its rooting rate up to 85.6%,
But the method takes a long time and the later period also needs to carry out acclimatization and transplants, completes to need 60d or more from taking root to acclimatization and transplants, fails to solve
Certainly Ormosia hosiei emerges slow problem, for this purpose, the present invention is using the method i.e. outside sprout-cultivating-bottle side being integrated with acclimatization and transplants of taking root
Method carries out taking root for Ormosia hosiei tissue-cultured seedling, and Ormosia hosiei breeding coefficient can be improved by exploring one, shortens emergence period, save the cost
High effective way is expanded by cultivation and is of great significance for quickly breeding Ormosia hosiei nursery stock.
Summary of the invention
The technical problem to be solved by the present invention is providing a kind of Ormosia hosiei tissue culture outside sprout-cultivating-bottle radication method, Ormosia hosiei is improved
Tissue-cultured seedling rooting rate shortens the emergence period and reduces production cost, to solve above-mentioned problems of the prior art.
The technical scheme adopted by the invention is as follows: a kind of Ormosia hosiei tissue culture outside sprout-cultivating-bottle radication method, this method include following step
It is rapid:
(1) sterilization: by Ormosia hosiei seedling stem section successively mass concentration be 0.1%KMnO4Middle sterilizing 10min, mass concentration
Ethyl alcohol 30s, mass concentration 0.1%HgCl for 75%28min is used aseptic water washing 4 ~ 5 times during two sterilizations；
(2) Initial culture: the seedling stem section after step (1) disinfection is cut into 1 ~ 1.5cm long in aseptic superclean bench, is connect
Kind is in following media: 1/2MS+TDZ0.02mg/L+NAA0.2mg/L+ sucrose 30g/L+ agar 8g/L carries out Fiber differentiation
Produce Multiple Buds；
(3) Multiplying culture: step (2) resulting Multiple Buds are cut into 1 ~ 1.5cm long in aseptic superclean bench, are inoculated in
In following media: WPM+6-BA1mg/L+NAA0.5mg/L+ sucrose 30g/L+ agar 8g/L carries out Multiplying culture；
(4) it outside sprout-cultivating-bottle culture: after the resulting proliferation seedling of step (3) is cut into single plant, is placed in NAA100-200mg/L and takes root
The liquid middling speed libation at an ancient wedding ceremony is inserted into the matrix disinfected without 10min is placed after 4 ~ 5s of offspring base portion, is grown 30 ~ 45d, is obtained rooted seedling；
Seedling stem section in step (1) after 90 DEG C of hot-water soaks for 24 hours, is placed in the pure leech of sterilization from Ormosia hosiei seed
The seedling stem section that 20d is sprouted in ground mass matter cultivates the less tender plant of children containing miscellaneous bacteria and is convenient for sterilization.
The culture medium respectively used in the incubation of step (2) and step (3) replaces primary identical culture at interval of 30d
Base avoids culture medium using overlong time, and moisture evaporation makes culture medium overdrying, influences the absorption of plant base portion normal nutrition.
Condition of culture in step (2) and step (3) are as follows: temperature (25 ± 2) DEG C, intensity of illumination 3000lx, light application time
6.0 are adjusted to for 12h/d, pH, culture place is Sterile culture room, provides Ormosia hosiei tissue cultures suitable condition of culture, in nothing
The culture of bacterium culturing room avoids air pollution.
Condition of culture in step (4) are as follows: greenhouse temperature (24 ± 2) DEG C, humidity 60% ~ 70% provide Ormosia hosiei bottle
The temperature and humidity condition for taking root outside suitable,.
The single plant cut in step (4), for height in 4 ~ 5cm, base portion is tiltedly cut into 45 DEG C, make the base portion of bud sufficiently with training
Base contact is supported, rooting rate is improved.
Matrix in step (4) is through autoclaving sterilization, and matrix is pressed the volume of 3:1:3 by peat soil, perlite, vermiculite
Than being mixed, matrix is watered to being sufficiently humidified so as to and use after mixing thoroughly, soil humidity 65%, cuttage is 10cm's in diameter
In plastic containers, plant is covered with preservative film, to keep soil humidity and reduce the moisture loss of plant.
Beneficial effects of the present invention: compared with prior art, the present invention using outside sprout-cultivating-bottle culture substrate (volume ratio 3:
The peat soil of 1:3: perlite: vermiculite) and the mode of NAA100-200mg/L taking root liquid carry out taking root for Ormosia hosiei tissue-cultured seedling, 30
After ~ 45d, rooting rate substantially increases rooting rate up to 97.78%, and Rooting ex vitro is higher than rooting rate in bottle by 10.41%, simplifies
Tissue cultures production process, shortens seedling-growing time, saves tissue cultures space, compared in vitro rooting, save the cost
55.77%。
Specific embodiment
The present invention is described further below with reference to specific embodiment.
Embodiment 1: a kind of Ormosia hosiei tissue culture outside sprout-cultivating-bottle radication method, method includes the following steps:
(1) by Ormosia hosiei seedling stem section successively mass concentration be 0.1%KMnO4Middle sterilizing 10min, the second that mass concentration is 75%
Alcohol 30s, mass concentration 0.1%HgCl28min is used aseptic water washing 5 times during two sterilizations；
(2) stem section for disinfecting step 1 cuts into 1.5cm long in superclean bench, is inoculated in 1/2MS+TDZ0.02mg/L
Inducing clumping bud is carried out in+NAA0.2mg/L culture medium；
(3) the resulting healthy and strong Multiple Buds of step 2 are cut into 1.5cm long in superclean bench, are inoculated in WPM+6-BA1mg/L
Multiplying culture is carried out in+NAA0.5mg/L culture medium；
(4) the resulting high 4cm stalwartness bud band tissue culture bottle of step 3 is moved under natural light after 3d, opens ventilated membrane and is refined under room temperature
Seedling 3d takes out base portion and tiltedly cuts at 45 °, is placed in the NAA150mg/L taking root liquid middling speed libation at an ancient wedding ceremony without placing 10min after offspring base portion 5s；
(5) bud cutting for handling step 4 well enters in nutritive cube, and the matrix that filling disinfects in nutritive cube, matrix is vermiculite: pearl
Rock (volume ratio 3:1), matrix are sufficiently humidified so as to, and cover preservative film, are scattered and disappeared with guaranteeing soil humidity and reducing plant moisture content, greenhouse temperature
24 DEG C of degree, humidity 65%, culture of rootage 40d.
Embodiment 2: a kind of Ormosia hosiei tissue culture outside sprout-cultivating-bottle radication method, method includes the following steps:
(1) by Ormosia hosiei seedling stem section successively mass concentration be 0.1%KMnO4Middle sterilizing 10min, the second that mass concentration is 75%
Alcohol 30s, mass concentration 0.1%HgCl28min is used aseptic water washing 5 times during two sterilizations；
(2) stem section for disinfecting step 1 cuts into 1.5cm long in superclean bench, is inoculated in 1/2MS+TDZ0.02mg/L
Inducing clumping bud is carried out in+NAA0.2mg/L culture medium；
(3) the resulting healthy and strong Multiple Buds of step 2 are cut into 1.5cm long in superclean bench, are inoculated in WPM+6-BA1mg/L
Multiplying culture is carried out in+NAA0.5mg/L culture medium；
(4) the resulting high 4cm stalwartness bud band tissue culture bottle of step 3 is moved under natural light after 3d, opens ventilated membrane and is refined under room temperature
Seedling 3d takes out base portion and tiltedly cuts at 45 °, is placed in the NAA200mg/L taking root liquid middling speed libation at an ancient wedding ceremony without placing 10min after offspring base portion 5s；
(5) bud cutting for handling step 4 well enters in nutritive cube, the matrix that filling disinfects in nutritive cube, and matrix is peat soil+treasure
Zhu Yan (volume ratio 3:1), matrix are sufficiently humidified so as to, and cover preservative film, are scattered and disappeared with guaranteeing soil humidity and reducing plant moisture content, greenhouse
24 DEG C of temperature, humidity 65%, culture of rootage 40d.
Embodiment 3: a kind of Ormosia hosiei tissue culture outside sprout-cultivating-bottle radication method, method includes the following steps:
(1) by Ormosia hosiei seedling stem section successively mass concentration be 0.1%KMnO4Middle sterilizing 10min, the second that mass concentration is 75%
Alcohol 30s, mass concentration 0.1%HgCl28min is used aseptic water washing 5 times during two sterilizations；
(2) stem section for disinfecting step 1 cuts into 1.5cm long in superclean bench, is inoculated in 1/2MS+TDZ0.02mg/L
Inducing clumping bud is carried out in+NAA0.2mg/L culture medium；
(3) the resulting healthy and strong Multiple Buds of step 2 are cut into 1.5cm long in superclean bench, are inoculated in WPM+6-BA1mg/L
Multiplying culture is carried out in+NAA0.5mg/L culture medium；
(4) the resulting high 4cm stalwartness bud band tissue culture bottle of step 3 is moved under natural light after 3d, opens ventilated membrane and is refined under room temperature
Seedling 3d takes out base portion and tiltedly cuts at 45 °, is placed in the NAA150mg/L taking root liquid middling speed libation at an ancient wedding ceremony without placing 10min after offspring base portion 5s；
(5) bud cutting for handling step 4 well enters in nutritive cube, and the matrix that filling disinfects in nutritive cube, matrix is peat soil: precious
Zhu Yan: vermiculite (volume ratio 3:1:3), matrix are sufficiently humidified so as to, and cover preservative film, are dissipated with guaranteeing soil humidity and reducing plant moisture content
It loses, 24 DEG C of greenhouse temperature, humidity 65%, culture of rootage 40d.
Embodiment 4: a kind of Ormosia hosiei tissue culture outside sprout-cultivating-bottle radication method, method includes the following steps:
(1) by Ormosia hosiei seedling stem section successively mass concentration be 0.1%KMnO4Middle sterilizing 10min, the second that mass concentration is 75%
Alcohol 30s, mass concentration 0.1%HgCl28min is used aseptic water washing 5 times during two sterilizations；
(2) stem section for disinfecting step 1 cuts into 1.5cm long in superclean bench, is inoculated in 1/2MS+TDZ0.02mg/L
Inducing clumping bud is carried out in+NAA0.2mg/L culture medium；
(3) the resulting healthy and strong Multiple Buds of step 2 are cut into 1.5cm long in superclean bench, are inoculated in WPM+6-BA1mg/L
Multiplying culture is carried out in+NAA0.5mg/L culture medium；
(4) the resulting high 4cm stalwartness bud band tissue culture bottle of step 3 is moved under natural light after 3d, opens ventilated membrane and is refined under room temperature
Seedling 3d takes out base portion and tiltedly cuts at 45 °, is placed in the NAA100mg/L taking root liquid middling speed libation at an ancient wedding ceremony without placing 10min after offspring base portion 5s；
(5) bud cutting for handling step 4 well enters in nutritive cube, and the matrix that filling disinfects in nutritive cube, matrix is peat soil: precious
Zhu Yan: vermiculite (volume ratio 3:1:3), matrix are sufficiently humidified so as to, and cover preservative film, are dissipated with guaranteeing soil humidity and reducing plant moisture content
It loses, 24 DEG C of greenhouse temperature, humidity 65%, culture of rootage 40d.
Embodiment 5: a kind of Ormosia hosiei tissue culture outside sprout-cultivating-bottle radication method, method includes the following steps:
(1) by Ormosia hosiei seedling stem section successively mass concentration be 0.1%KMnO4Middle sterilizing 10min, the second that mass concentration is 75%
Alcohol 30s, mass concentration 0.1%HgCl28min is used aseptic water washing 5 times during two sterilizations；
(2) stem section for disinfecting step 1 cuts into 1.5cm long in superclean bench, is inoculated in 1/2MS+TDZ0.02mg/L
Inducing clumping bud is carried out in+NAA0.2mg/L culture medium；
(3) the resulting healthy and strong Multiple Buds of step 2 are cut into 1.5cm long in superclean bench, are inoculated in WPM+6-BA1mg/L
Multiplying culture is carried out in+NAA0.5mg/L culture medium；
(4) the resulting high 4cm stalwartness bud band tissue culture bottle of step 3 is moved under natural light after 3d, opens ventilated membrane and is refined under room temperature
Seedling 3d takes out base portion and tiltedly cuts 45 °, is placed in the NAA200mg/L taking root liquid middling speed libation at an ancient wedding ceremony without placing 10min after offspring base portion 5s；
(5) bud cutting for handling step 4 well enters in nutritive cube, and the matrix that filling disinfects in nutritive cube, matrix is peat soil: precious
Zhu Yan: vermiculite (volume ratio 3:1:3), matrix are sufficiently humidified so as to, and cover preservative film, are dissipated with guaranteeing soil humidity and reducing plant moisture content
It loses, 24 DEG C of greenhouse temperature, humidity 65%, culture of rootage 40d.
In order to illustrate effect of the invention, following comparative test is carried out, as shown in table 1:
The result of Rooting ex vitro is obtained from 1 different substrates of table and taking root liquid concentration, in matrix vermiculite+perlite (3:1) and
Peat soil: perlite: carrying out that outside sprout-cultivating-bottle effect is preferable, and rooting rate is respectively 91.11% and 97.78% on vermiculite (3:1:3),
Worst matrix is peat soil+perlite (3:1), average rooting rate only 40%；Using the various concentration NAA speed libation at an ancient wedding ceremony handle in, with
150mg/L effect is preferable, and average rooting rate is 71.11%, respectively than 100mg/L, 200mg/L high 13.33% and 2.22%.
It can be seen that different substrates and NAA concentration from 2 the results of analysis of variance of table and there is extremely significant shadow to outside sprout-cultivating-bottle rate
Ring (P< 0.01).
To sum up sample result obtains, the method for the optimal taking root liquid of Ormosia hosiei outside sprout-cultivating-bottle and substrate combination is
NAA150mg/L takes root the liquid speed libation at an ancient wedding ceremony without offspring base portion 5s, places 10min, is placed in matrix peat soil: perlite: vermiculite (3:1:3)
In take root, keep soil humidity 65%, rooting rate is up to 97.78%.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
Those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, and should all contain
Within protection scope of the present invention, therefore, protection scope of the present invention should be based on the protection scope of the described claims lid.
Claims (7)
1. a kind of Ormosia hosiei tissue culture outside sprout-cultivating-bottle radication method, it is characterised in that: method includes the following steps:
(1) sterilization: by Ormosia hosiei seedling stem section successively mass concentration be 0.1%KMnO4Middle sterilizing 10min, mass concentration
For 75% ethyl alcohol sterilizing 0.5min, mass concentration 0.1%HgCl2Sterilize 8min, is rushed during two sterilizations with sterile water
It washes 4 ~ 5 times；
(2) Initial culture: the stem section after step (1) disinfection is cut into 1 ~ 1.5cm long in aseptic superclean bench, is inoculated in
In following media: 1/2MS+TDZ0.02mg/L+NAA0.2mg/L+ sucrose 30g/L+ agar 8g/L carries out Fiber differentiation production
Multiple Buds；
(3) Multiplying culture: step (2) resulting Multiple Buds are cut into 1 ~ 1.5cm long in aseptic superclean bench, are inoculated in
In following media: WPM+6-BA1mg/L+NAA0.5mg/L+ sucrose 30g/L+ agar 8g/L carries out Multiplying culture and is proliferated
Seedling；
(4) it outside sprout-cultivating-bottle culture: after the resulting proliferation seedling of step (3) is cut into single plant, is placed in NAA100 ~ 200mg/L and takes root
The liquid middling speed libation at an ancient wedding ceremony is inserted into the matrix disinfected without 10min is placed after 4 ~ 5s of offspring base portion, is grown 30 ~ 45d, is obtained rooted seedling.
2. a kind of Ormosia hosiei tissue culture outside sprout-cultivating-bottle radication method according to claim 1, it is characterised in that: step (1) is described
Seedling stem section acquisition methods are as follows: Ormosia hosiei seed is placed in the pure vermiculite matrix of sterilization after 90 DEG C of hot-water soaks for 24 hours
Sprout 20d, the seedling stem section higher than 12cm.
3. Ormosia hosiei tissue culture outside sprout-cultivating-bottle radication method according to claim 1, which is characterized in that step (2) and step (3)
The culture medium respectively used in incubation replaces a same medium at interval of 30d.
4. Ormosia hosiei tissue culture outside sprout-cultivating-bottle radication method according to claim 1, which is characterized in that step (2) and step (3)
Middle condition of culture is equal are as follows: 23 ~ 27 DEG C of temperature, intensity of illumination 3000lx, light application time 12h/d, pH are adjusted to 6.0, cultivate place
For Sterile culture room.
5. Ormosia hosiei tissue culture outside sprout-cultivating-bottle radication method according to claim 1, which is characterized in that bottle is external in step (4)
Root condition of culture are as follows: 22-26 DEG C of greenhouse temperature, humidity 60% ~ 70%.
6. Ormosia hosiei tissue culture outside sprout-cultivating-bottle radication method according to claim 1, which is characterized in that step is cut in (4)
Single plant, height in 4 ~ 5cm, base portion is tiltedly cut at 45 °.
7. Ormosia hosiei tissue culture outside sprout-cultivating-bottle radication method according to claim 1, which is characterized in that step (4) mesostroma is
Through autoclaving sterilization, matrix is mixed by peat soil, perlite, vermiculite by the volume ratio of 3:1:3, and matrix is watered to wet
Moisten and used after mixing thoroughly, soil humidity 65%, cuttage covers plant in the plastic containers for filling matrix of 10cm, with preservative film
Strain.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811309483.8A CN109220805A (en) | 2018-11-05 | 2018-11-05 | A kind of Ormosia hosiei tissue culture outside sprout-cultivating-bottle radication method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811309483.8A CN109220805A (en) | 2018-11-05 | 2018-11-05 | A kind of Ormosia hosiei tissue culture outside sprout-cultivating-bottle radication method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109220805A true CN109220805A (en) | 2019-01-18 |
Family
ID=65076743
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811309483.8A Pending CN109220805A (en) | 2018-11-05 | 2018-11-05 | A kind of Ormosia hosiei tissue culture outside sprout-cultivating-bottle radication method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109220805A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111512808A (en) * | 2019-10-30 | 2020-08-11 | 福建农林大学 | Method for rapidly inducing adventitious root regeneration of brachypodium distachyon tissue culture seedlings |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009158694A1 (en) * | 2008-06-28 | 2009-12-30 | The Donald Danforth Plant Science Center | Improved protein production and storage in plants |
| CN102657088A (en) * | 2012-05-11 | 2012-09-12 | 中国林业科学研究院亚热带林业研究所 | Tissue culture method for Ormosia hosiei et Wils |
| CN103444529A (en) * | 2013-08-17 | 2013-12-18 | 福建农林大学 | Method for reproduction and mass propagation of lower axis fragment plants of ormosia hosiei.et wils |
| CN103461126A (en) * | 2013-09-17 | 2013-12-25 | 南京通泽农业科技有限公司 | Rapid propagation method for palm trees |
-
2018
- 2018-11-05 CN CN201811309483.8A patent/CN109220805A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009158694A1 (en) * | 2008-06-28 | 2009-12-30 | The Donald Danforth Plant Science Center | Improved protein production and storage in plants |
| CN102657088A (en) * | 2012-05-11 | 2012-09-12 | 中国林业科学研究院亚热带林业研究所 | Tissue culture method for Ormosia hosiei et Wils |
| CN103444529A (en) * | 2013-08-17 | 2013-12-18 | 福建农林大学 | Method for reproduction and mass propagation of lower axis fragment plants of ormosia hosiei.et wils |
| CN103461126A (en) * | 2013-09-17 | 2013-12-25 | 南京通泽农业科技有限公司 | Rapid propagation method for palm trees |
Non-Patent Citations (4)
| Title |
|---|
| 桂平等: "珍稀树种红豆树茎段启动培养的影响因素", 《西南农业学报》 * |
| 段如雁等: "花榈木容器育苗的基质筛选", 《林业科技开发》 * |
| 程家胜: "《植物组织培养与工厂化育苗技术》", 31 March 2003, 金盾出版社 * |
| 魏岩: "《园林苗木生产》", 31 August 2011, 沈阳出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111512808A (en) * | 2019-10-30 | 2020-08-11 | 福建农林大学 | Method for rapidly inducing adventitious root regeneration of brachypodium distachyon tissue culture seedlings |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105638477A (en) | Rapid propagation method for dendrobium hancockii seeds | |
| CN105993865A (en) | Cultivation method for quercus variabilis aseptic seedling | |
| CN106718883A (en) | A kind of Yunnan Chinese catalpa and the miniature engrafting method of test tube seedling of Chinese catalpa | |
| TWI235031B (en) | Process for producing orchid seedlings by static liquid culture | |
| CN107094625A (en) | A kind of southerm yew tissue culture of sprout mating system | |
| CN105165627B (en) | A kind of rosewood tissue culture sterilization formula and tissue culture method | |
| CN105474992A (en) | Chinese chestnut summer truffle mycorrhizal seedling culture method based on symbiotic relation | |
| CN105766654B (en) | A kind of Nanchuan jackfruit method for tissue culture | |
| CN105850741A (en) | Rapid propagation and in-vitro preservation method of coniogramme japonica (Thunberg) diels | |
| CN107018905B (en) | A kind of bottle orchid test tube seedling preserving seed method | |
| CN106577280B (en) | A method of aseptic seedling is quickly bred using Merrillanthus hainanensis tender stem segments and blade | |
| CN108834894A (en) | A kind of method for tissue culture of uncaria | |
| CN109220805A (en) | A kind of Ormosia hosiei tissue culture outside sprout-cultivating-bottle radication method | |
| CN104920218B (en) | The propagation method of delavay pararuellia herb | |
| CN105409748B (en) | A kind of fast breeding method of extra large scirpus scirpus | |
| Santana et al. | Effect of different culture tube caps and concentrations of activated charcoal and sucrose on in vitro growth and budding induction of Annona glabra L. | |
| CN103141384A (en) | Rapid tissue culture propagation method of pot chrysanthemum cultivars | |
| CN103621399A (en) | Peony tissue culture method | |
| CN109247235A (en) | A kind of orchid fast seedling raising method | |
| CN105993955B (en) | A kind of false-yellowflower milkwort root or herb rapid propagation in vitro method for culturing seedlings | |
| CN105684910B (en) | The tissue culture and rapid propagation method of dendrobium hancockii Rolfe | |
| CN105230488B (en) | A kind of Cymbidium lancifolium leaf tissue culture method for quickly breeding | |
| CN105830928B (en) | A kind of method of Herba Tricyrtidis macropodae tissue culture and rapid proliferation | |
| CN107960325A (en) | A kind of tissue culture method of forget-me-not | |
| CN107494266B (en) | A kind of anti-browning of Tokyo Dendronenthamia japonica var.chinensis and tissue culture enrichment procedure |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |