CN105474992A - Chinese chestnut summer truffle mycorrhizal seedling culture method based on symbiotic relation - Google Patents
Chinese chestnut summer truffle mycorrhizal seedling culture method based on symbiotic relation Download PDFInfo
- Publication number
- CN105474992A CN105474992A CN201510844850.4A CN201510844850A CN105474992A CN 105474992 A CN105474992 A CN 105474992A CN 201510844850 A CN201510844850 A CN 201510844850A CN 105474992 A CN105474992 A CN 105474992A
- Authority
- CN
- China
- Prior art keywords
- seedling
- chinese chestnut
- matrix
- inoculation
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/10—Mycorrhiza; Mycorrhizal associations
Abstract
The invention discloses a Chinese chestnut summer truffle mycorrhizal seedling culture method based on the symbiotic relation. The method specifically includes the following steps that semi-aseptic seedlings are cultured, and Chinese chestnut semi-aseptic seedlings are obtained through preparation; an inoculation solution and an inoculation matrix are prepared, and seedling culture trays are prepared; summer truffle inoculation treatment is performed; post-inoculation management is performed; the humidity of the matrix is controlled, proper ventilation is performed, and a certain temperature and a certain air humidity are kept; mycorrhizae are checked and planted outdoors. According to the culture method, the mycorrhizal seedlings can increase the survival rate of afforestation of Chinese chestnut trees; the mycorrhizae can increase the absorption area of root systems of the Chinese chestnut trees, promote growth and development of the Chinese chestnut trees and increase the yield of Chinese chestnuts; the Chinese chestnut summer truffle mycorrhizal seedlings achieve the overground and underground joint earnings, increase the utilization rate of land and increase the economic benefit per unit land area.
Description
Technical field
The invention belongs to forest economy woods seedling growing process field and microbial technology field, be specifically related to a kind of breeding method of the Chinese chestnut summer truffle Va Mycorrhiza Seedling based on symbiotic relation.
Background technology
The current whole nation is cultivated Chestnut Seedlings and is all adopted conventional seedbed system technology, and after nursery stock afforestation, 5-7 chestnut tree is born fruit, and economic well-being of workers and staff only has Chinese chestnut, and unit are economic benefit source is single.Further, ferfas is under the jurisdiction of Ascomycetes (Ascomycets) Tuberale (Tubrales) Tuberaceae (Tuberaceae) Tuber (TuberF.H.Wigg) at present only by nature wild resource supply market.Due to the particularity of ferfas growth, do great damage to the bacterium pool and ecotope when excavating, ferfas output is sharply declined, and output can not be met the need of market far away.Chinese chestnut is the symbiosis seeds of ferfas, and for improving land utilization rate, increase economic well-being of workers and staff, the two is organically combined, change seedlings of Chinese chestnut breeding method, obtain Chinese chestnut summer truffle Va Mycorrhiza Seedling, experimental study obtains the seedling growing process of complete set.
Existing existing raising seedling of chestnut technology is routine techniques, another existing ferfas seeding cultivating method is mainly used in pinaceae plant, mainly there is following subject matter: the Chestnut Seedlings of existing afforestation is the common seedling not having symbiosis mycorrhiza substantially, the chestnut wood that common seedling is made will only have acrial part income future; Pinaceae plant economic benefit is low.
Summary of the invention
The object of this invention is to provide a kind of breeding method of the Chinese chestnut summer truffle Va Mycorrhiza Seedling based on symbiotic relation, solve, the Chinese chestnut kind poor without symbiosis mycorrhiza, seedling medium ventilating permeable that exist in existing raising seedling of chestnut technology and store difficult, that seedling raise period is long problem.
The technical solution adopted in the present invention is, a kind of breeding method of the Chinese chestnut summer truffle Va Mycorrhiza Seedling based on symbiotic relation, specifically implements according to following steps:
1) cultivate half aseptic seedling, prepare Chinese chestnut half aseptic seedling;
2) prepare before inoculation: prepare inoculation liquid, preparation inoculation matrix and prepare seedling-cultivating tray;
3) summer truffle inoculation process is carried out;
4) carry out inoculating rear management, control soil humidity, suitably ventilate, breathe freely, keep certain temperature and air humidity;
5) mycorhiza inspection and go out plant.
Further, cultivate Chinese chestnut half aseptic seedling in step 1 to be specially:
1.1) India truffle that market purchasing is fresh, ripe, cleans silt and loads valve bag, is placed in-18 DEG C of cryogenic freezing storages; And select to form mycorhiza with ferfas, produce the Chinese chestnut of fruit body;
1.2) matrix prepares: be that 1:1 mixes by the tobacco floating seedling culture matrix of vermiculite, YC/T310-2009 standard with volume ratio, and add suitable quantity of water and mix evenly, moisture content about 28%, namely matrix has been pinched agglomerating, scatters as well after loosing one's grip; Matrix 121 DEG C, under the HTHP of 110kPa sterilizing cool stand-by after 2 hours;
1.3) seedling-cultivating tray and the sterilization of basin alms bowl: soak 2 hours with 5 ‰ liquor potassic permanganates, use aseptic water washing three times after taking out;
1.4) removing of Castanea Mollissima Seeds wet cleaning process empty flat after put into 30% hydrogen peroxide sterilizing 40 minutes, pick up be sown into after aseptic water washing fill sterilization matrix basin alms bowl or seedling-cultivating tray in, with cooled boiling water or distilled water pouring, be placed in greenhouse and cultivate, prepare Chinese chestnut half aseptic seedling; In Second Year 2-4 month, as the long 5-10cm of Chinese chestnut axial root system, after the root prosperity of side, start inoculation.
Further, prepare inoculation liquid, preparation inoculation matrix in step 2 and prepare seedling-cultivating tray and be specially:
2.1) preparation of inoculation liquid: fruit juice mixer with after the rayon balls wiping of 75% alcohol, with aseptic water washing, 3 times repeatedly; After ripe ferfas is cleaned with running water, then use the rayon balls wiped clean of 75% alcohol, to prune crust with sterile scalpel, be cut into small pieces; Put into ready fruit juice mixer and add 400-500ml sterile water, open fruit juice machine stirrer and be about 25-30 second, make the spore suspension that kames concentration is 10%, pour in sterilized bottle, sealing, puts 4 DEG C of Refrigerator stores for subsequent use;
2.2), substrate preparation is inoculated: in autoclaved matrix, add appropriate amounts of sterilized water mix thoroughly, mix thoroughly according to adding quantitative quicklime after the volume computing of matrix, pH value is transferred between 7.0-8.0, matrix has been pinched agglomerating, scatters as well after loosing one's grip;
2.3), film and seedling-cultivating tray prepare: sheet to be inserted in hydrogen peroxide sterilizing 40 minutes, taking-up aqua sterilisa is rinsed well for subsequent use; Seedling-cultivating trays and basin alms bowl 2 hours is soaked, with aseptic water washing 3-5 time after taking out with 5 ‰ liquor potassic permanganates.
Further, inoculate process to be specially:
3.1), by the matrix prepared be laid on the sheet of sterilizing, take out Chinese chestnut half aseptic seedling, root is placed in the middle part of matrix, and root is fully trailed;
3.2), draw the spore suspension 10ml prepared with suction pipe, drench equably and wrap sheet on root, sheet degree of tightness is applicable to, and matrix can not be scattered;
3.3) put into ready seedling-cultivating tray successively, water appropriate distilled water, be placed in greenhouse and cultivate.Notice that water can not water too much, otherwise ferfas spore can be washed away.
Further, the inoculation post processing in step 4 is specially:
4.1), water: with cold boiling water or distilled water pouring between culture period, keep matrix moistening;
4.2), temperature: process of need sheltering from heat or light summer, keep mean temperature 18.64 DEG C in greenhouse, maximum temperature is no more than 32 DEG C, is minimumly not less than 1 DEG C;
4.3), humidity: medial humidity 64% in greenhouse, maximal humidity is no more than 96%, and minimum humidity is not less than 34%.
Further, after seedling inoculation 1-2 month starts to check, mainly observe growth and the form of mycorhiza with magnifying glass, the Va Mycorrhiza Seedling infecting other miscellaneous bacterias need remove immediately, pollutes in order to avoid expand; Second time inspection: inoculate latter 6 months, inspection mycorhiza infection rate, mycorhiza is few, or need the carrying out second time in 2-4 month or two time periods of 7-8 month and mend inoculation of sterilized root; Third time inspection: inspection before Va Mycorrhiza Seedling goes out to plant, select mycorhiza infection rate to reach 60%, Mycorrhizal Development is good, and the vivid Va Mycorrhiza Seedling of color is to field field planting.
The invention has the beneficial effects as follows: 1, by adjustment seedling medium proportioning and pH value, improve matrix ventilating permeable, seedling root growth preferably and mycorhiza are formed and grow.
2, change Chinese chestnut conventional seed planting time raising Chinese chestnut emergence rate, autumn sowing Castanea Mollissima Seeds solves Chinese chestnut and stores difficult and shorten seedling raise period.
3, change Castanea Mollissima Seeds processing method and save human and material resources, seed disinfection processing method: 30% hydrogen peroxide dipping 40 minutes, with aseptic water washing 3-4 rear directly sowing, save manpower than the mechanical cut of employing and alcohol wipe and avoid secondary pollution.
4, pass through after inoculation to control seedling raising environment temperature, light, gas, wet, promote growing of nursery stock mycorhiza, before guaranteeing the field planting of cultivation field, mycorhiza infection rate reaches more than 60%.
In breeding method of the present invention, Va Mycorrhiza Seedling afforestation can improve Chinese chestnut survival rate; Mycorhiza can improve Chinese chestnut Root Absorption ability, promotes that chestnut tree grows; Chinese chestnut summer truffle Va Mycorrhiza Seedling finally reaches on the ground, underground community income, improves land utilization rate, increases Land Units area economic benefit.
Embodiment
Below in conjunction with embodiment, the present invention is described in detail.
The invention provides a kind of breeding method of the Chinese chestnut summer truffle Va Mycorrhiza Seedling based on symbiotic relation, specifically implement according to following steps:
1, Chinese chestnut half aseptic seedling is cultivated;
1.1, summer truffle (Tuberaestivum) fungi preservation: the summer truffle that market purchasing is fresh, ripe, cleans silt and loads valve bag, is placed in-18 DEG C of cryogenic freezing storages;
Symbiosis choice of tree species: select to form mycorhiza with ferfas, produce the Chinese chestnut (Castaneamollissima) of fruit body;
1.2, matrix prepares: be that 1:1 mixes by the tobacco floating seedling culture matrix of vermiculite, YC/T310-2009 standard with volume ratio, and add suitable quantity of water and mix evenly, moisture content about 28%, namely matrix has been pinched agglomerating, scatters as well after loosing one's grip.Matrix 121 DEG C, under the HTHP of 110kPa sterilizing cool stand-by after 2 hours;
1.3, seedling-cultivating tray and the sterilization of basin alms bowl: soak 2 hours with 5 ‰ liquor potassic permanganates, use aseptic water washing 3-5 time after taking out;
1.4, the removing of Castanea Mollissima Seeds wet cleaning process empty flat after put into 30% hydrogen peroxide sterilizing 40 minutes, pick up be sown into after aseptic water washing fill sterilization matrix basin alms bowl or seedling-cultivating tray in, with cooled boiling water or distilled water pouring, be placed in greenhouse and cultivate, obtain Chinese chestnut half aseptic seedling; In Second Year 2-4 month, as the long 5-10cm of Chinese chestnut axial root system, after the root prosperity of side, start inoculation;
2, prepare before inoculation
2.1, material prepares: summer truffle (Tuberaestivum) mature sporophore, alcolhol burner 1,2, pocket knife, 2, scissors, sheet, emgloves 10 pairs, 1,30 times of magnifying glasses;
2.2, the preparation of inoculation liquid: fruit juice mixer with after the rayon balls wiping of 75% alcohol, with aseptic water washing, 3 times repeatedly; After ripe ferfas is cleaned with running water, then use the rayon balls wiped clean of 75% alcohol, to prune crust with sterile scalpel, be cut into small pieces; Put into ready fruit juice mixer and add 400-500ml sterile water, open fruit juice machine stirrer and be about 25-30 second, making summer truffle fruit body concentration is the spore suspension of 10%, pours in sterilized bottle, and sealing, puts 4 DEG C of Refrigerator stores for subsequent use;
2.3, substrate preparation is inoculated: in autoclaved matrix, add appropriate amounts of sterilized water mix thoroughly, mix thoroughly according to adding quantitative quicklime after the volume computing of matrix, pH value is transferred between 7.0-8.0, matrix has been pinched agglomerating, scatters as well after loosing one's grip;
2.4, sheet and seedling-cultivating tray prepare: according to the size of seedling-culturing dish cave, sheet is cut into applicable size, and to insert in hydrogen peroxide sterilizing 40 minutes, taking-up aqua sterilisa is rinsed well for subsequent use.Seedling-cultivating tray sterilization is with 1.3;
3, inoculate:
3.1, be laid on the sheet of sterilizing by the matrix prepared, take out Chinese chestnut half aseptic seedling, root is placed in the middle part of matrix, and fibrous root is fully trailed;
3.2, with suction pipe draw prepare in advance spore suspension 10ml, drench equably and wrap sheet on fibrous root, sheet degree of tightness be applicable to, matrix can not be scattered;
3.3, the seedling wrapped by sheet together puts into ready seedling-cultivating tray together with sheet, gently waters and drenches appropriate distilled water, is placed in greenhouse and cultivates.Notice that water can not water too much, otherwise ferfas spore can be washed away;
4, management after inoculation
Postvaccinal management mainly controls soil humidity, suitably ventilates, breathes freely, keep certain temperature and air humidity;
4.1 water: with cold boiling water or distilled water pouring between culture period, keep matrix moistening;
4.2 temperature: process of need lowering the temperature summer, keep mean temperature 18.64 DEG C in greenhouse, maximum temperature is no more than 32 DEG C, is minimumly not less than 1 DEG C;
4.3 humidity: average air humidity 64% in greenhouse, are the highlyest no more than 96%, are minimumly not less than 34%.
5, mycorhiza inspection
After seedling inoculation 1-2 month starts to check, mainly observe growth and the form of mycorhiza with magnifying glass, the Va Mycorrhiza Seedling infecting other miscellaneous bacterias need remove immediately, pollutes in order to avoid expand; Second time inspection: inoculate latter 6 months, inspection mycorhiza infection rate, mycorhiza is few, or need the carrying out second time in 2-4 month or two time periods of 7-8 month and mend inoculation of sterilized root; Third time inspection: inspection Mycorrhizal Development situation before Va Mycorrhiza Seedling goes out to plant, select mycorhiza infection rate to reach 60%, Mycorrhizal Development is good, and the Va Mycorrhiza Seedling that color is vivid goes out to plant.Summer truffle Mycorrhizal Technology educates Chestnut Seedlings and common contrast of educating Chestnut Seedlings in table 1.
Table 1 summer truffle Mycorrhizal Technology educates Chestnut Seedlings and common contrast of educating Chestnut Seedlings
Claims (6)
1., based on a breeding method for the Chinese chestnut summer truffle Va Mycorrhiza Seedling of symbiotic relation, it is characterized in that, specifically implement according to following steps:
1) cultivate half aseptic seedling, prepare Chinese chestnut half aseptic seedling;
2) prepare before inoculation: prepare inoculation liquid, preparation inoculation matrix and prepare seedling-cultivating tray;
3) summer truffle inoculation process is carried out;
4) carry out inoculating rear management, control soil humidity, suitably ventilate, breathe freely, keep certain temperature and air humidity;
5) mycorhiza inspection and go out plant.
2. the breeding method of the Chinese chestnut summer truffle Va Mycorrhiza Seedling based on symbiotic relation according to claim 1, is characterized in that, cultivates Chinese chestnut half aseptic seedling and be specially in described step 1:
1.1) India truffle that market purchasing is fresh, ripe, cleans silt and loads valve bag, is placed in-18 DEG C of cryogenic freezing storages; And select to form mycorhiza with ferfas, produce the Chinese chestnut of fruit body;
1.2) matrix prepares: be that 1:1 mixes by the tobacco floating seedling culture matrix of vermiculite, YC/T310-2009 standard with volume ratio, and add suitable quantity of water and mix evenly, moisture content about 28%, namely matrix has been pinched agglomerating, scatters as well after loosing one's grip; Matrix 121 DEG C, under the HTHP of 110kPa sterilizing cool stand-by after 2 hours;
1.3) seedling-cultivating tray and the sterilization of basin alms bowl: soak 2 hours with 5 ‰ liquor potassic permanganates, use aseptic water washing three times after taking out;
1.4) removing of Castanea Mollissima Seeds wet cleaning process empty flat after put into 30% hydrogen peroxide sterilizing 40 minutes, pick up be sown into after aseptic water washing fill sterilization matrix basin alms bowl or seedling-cultivating tray in, with cooled boiling water or distilled water pouring, be placed in greenhouse and cultivate, prepare Chinese chestnut half aseptic seedling; In Second Year 2-4 month, as the long 5-10cm of Chinese chestnut axial root system, after the root prosperity of side, start inoculation.
3. the breeding method of the Chinese chestnut summer truffle Va Mycorrhiza Seedling based on symbiotic relation according to claim 2, is characterized in that, prepares inoculation liquid, preparation inoculation matrix and prepare seedling-cultivating tray to be specially in described step 2:
2.1) preparation of inoculation liquid: fruit juice mixer with after the rayon balls wiping of 75% alcohol, with aseptic water washing, 3 times repeatedly; After ripe ferfas is cleaned with running water, then use the rayon balls wiped clean of 75% alcohol, to prune crust with sterile scalpel, be cut into small pieces; Put into ready fruit juice mixer and add 400-500ml sterile water, open fruit juice machine stirrer and be about 25-30 second, make the spore suspension that kames concentration is 10%, pour in sterilized bottle, sealing, puts 4 DEG C of Refrigerator stores for subsequent use;
2.2) substrate preparation is inoculated: in the matrix of sterilizing, add appropriate amounts of sterilized water mix thoroughly, mix thoroughly according to adding quantitative quicklime after the volume computing of matrix, pH value is transferred between 7.0-8.0, matrix has been pinched agglomerating, scatters as well after loosing one's grip;
2.3) sheet and seedling-cultivating tray prepare: sheet to be inserted in hydrogen peroxide sterilizing 40 minutes, taking-up aqua sterilisa is rinsed well for subsequent use; Seedling-cultivating trays and basin alms bowl 2 hours is soaked, with aseptic water washing 3-5 time after taking out with 5 ‰ liquor potassic permanganates.
4. the breeding method of the Chinese chestnut summer truffle Va Mycorrhiza Seedling based on symbiotic relation according to claim 3, it is characterized in that, described inoculation process is specially:
3.1) be laid on the sheet of sterilizing by the matrix prepared, take out Chinese chestnut half aseptic seedling, root is placed in the middle part of matrix, and root is fully trailed;
3.2) draw the spore suspension 10ml prepared with suction pipe, drench equably and wrap sheet on root, sheet degree of tightness is applicable to, and matrix can not be scattered;
3.3) put into ready seedling-cultivating tray successively, water appropriate distilled water, be placed in greenhouse and cultivate, notice that water can not water too much, otherwise ferfas spore can be washed away.
5. the breeding method of the Chinese chestnut summer truffle Va Mycorrhiza Seedling based on symbiotic relation according to claim 4, it is characterized in that, the inoculation post processing in described step 4 is specially:
4.1) water: with cold boiling water or distilled water pouring between culture period, keep matrix moistening;
4.2) temperature: process of need sheltering from heat or light summer, keep mean temperature 18.64 DEG C in greenhouse, maximum temperature is no more than 32 DEG C, is minimumly not less than 1 DEG C;
4.3) humidity: medial humidity 64% in greenhouse, maximal humidity is no more than 96%, and minimum humidity is not less than 34%.
6. the breeding method of the Chinese chestnut summer truffle Va Mycorrhiza Seedling based on symbiotic relation according to claim 5, it is characterized in that, after described seedling inoculation 1-2 month starts to check, growth and the form of mycorhiza is mainly observed with magnifying glass, the Va Mycorrhiza Seedling infecting other miscellaneous bacterias need remove immediately, pollutes in order to avoid expand; Second time inspection: inoculate latter 6 months, inspection mycorhiza infection rate, mycorhiza is few, or need the carrying out second time in 2-4 month or two time periods of 7-8 month and mend inoculation of sterilized root; Third time inspection: inspection before Va Mycorrhiza Seedling goes out to plant, select mycorhiza infection rate to reach 60%, Mycorrhizal Development is good, and the vivid Va Mycorrhiza Seedling of color is to field field planting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510844850.4A CN105474992A (en) | 2015-11-28 | 2015-11-28 | Chinese chestnut summer truffle mycorrhizal seedling culture method based on symbiotic relation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510844850.4A CN105474992A (en) | 2015-11-28 | 2015-11-28 | Chinese chestnut summer truffle mycorrhizal seedling culture method based on symbiotic relation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105474992A true CN105474992A (en) | 2016-04-13 |
Family
ID=55662578
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510844850.4A Pending CN105474992A (en) | 2015-11-28 | 2015-11-28 | Chinese chestnut summer truffle mycorrhizal seedling culture method based on symbiotic relation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105474992A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105917913A (en) * | 2016-04-28 | 2016-09-07 | 四川省农业科学院土壤肥料研究所 | Method for breeding mycorrhizal seedlings by truffles and quercus acutissima |
| CN106171515A (en) * | 2016-07-08 | 2016-12-07 | 云南大学 | A kind of Applying Ectomycorrhizal Fungi and the symbiosis method for building up of pine genus plant |
| CN107027519A (en) * | 2017-06-05 | 2017-08-11 | 攀枝花市农林科学研究院 | The mycorrhiza fungi seeding cultivating method of Panzhihua ferfas |
| CN107231940A (en) * | 2017-06-05 | 2017-10-10 | 攀枝花市农林科学研究院 | Ferfas is inoculated with bacterium ball and its preparation and application |
| CN107333566A (en) * | 2017-08-17 | 2017-11-10 | 佛山推启农业研究院（普通合伙） | A kind of method that russule is cultivated using Asiatic plantain Va Mycorrhiza Seedling |
| CN109937884A (en) * | 2019-04-26 | 2019-06-28 | 北京农学院 | A kind of method of Chinese chestnut and the in vitro symbiosis culture of fungi |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007029072A (en) * | 2005-07-27 | 2007-02-08 | Morika Iwai | Method for culturing sandalwood of phellinus linteus |
| CN101061781A (en) * | 2007-04-23 | 2007-10-31 | 攀枝花市农林科学研究院 | Chinese truffle and India truffle mycorrhiza fungi seeding cultivating method |
| CN101595803A (en) * | 2008-06-03 | 2009-12-09 | 上海市农业科学院 | A kind of production method of mycorhiza edible fungi offspring |
| CN103070014A (en) * | 2013-01-30 | 2013-05-01 | 攀枝花市林业科学技术推广站（攀枝花市林业工作总站） | Method for breeding summer truffle root seedling through inoculation of suspension liquid |
| CN103070013A (en) * | 2013-01-30 | 2013-05-01 | 攀枝花市林业科学技术推广站（攀枝花市林业工作总站） | Method for manually inoculating and culturing root seedling of summer truffle |
| CN103749154A (en) * | 2014-01-13 | 2014-04-30 | 攀枝花市丰盛源农林开发有限公司 | Culture medium for promoting cross infection among truffle mycorrhizas and using method thereof |
-
2015
- 2015-11-28 CN CN201510844850.4A patent/CN105474992A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007029072A (en) * | 2005-07-27 | 2007-02-08 | Morika Iwai | Method for culturing sandalwood of phellinus linteus |
| CN101061781A (en) * | 2007-04-23 | 2007-10-31 | 攀枝花市农林科学研究院 | Chinese truffle and India truffle mycorrhiza fungi seeding cultivating method |
| CN101595803A (en) * | 2008-06-03 | 2009-12-09 | 上海市农业科学院 | A kind of production method of mycorhiza edible fungi offspring |
| CN103070014A (en) * | 2013-01-30 | 2013-05-01 | 攀枝花市林业科学技术推广站（攀枝花市林业工作总站） | Method for breeding summer truffle root seedling through inoculation of suspension liquid |
| CN103070013A (en) * | 2013-01-30 | 2013-05-01 | 攀枝花市林业科学技术推广站（攀枝花市林业工作总站） | Method for manually inoculating and culturing root seedling of summer truffle |
| CN103749154A (en) * | 2014-01-13 | 2014-04-30 | 攀枝花市丰盛源农林开发有限公司 | Culture medium for promoting cross infection among truffle mycorrhizas and using method thereof |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105917913A (en) * | 2016-04-28 | 2016-09-07 | 四川省农业科学院土壤肥料研究所 | Method for breeding mycorrhizal seedlings by truffles and quercus acutissima |
| CN106171515A (en) * | 2016-07-08 | 2016-12-07 | 云南大学 | A kind of Applying Ectomycorrhizal Fungi and the symbiosis method for building up of pine genus plant |
| CN106171515B (en) * | 2016-07-08 | 2019-03-19 | 云南大学 | A kind of symbiosis method for building up of Applying Ectomycorrhizal Fungi and pine genus plant |
| CN107027519A (en) * | 2017-06-05 | 2017-08-11 | 攀枝花市农林科学研究院 | The mycorrhiza fungi seeding cultivating method of Panzhihua ferfas |
| CN107231940A (en) * | 2017-06-05 | 2017-10-10 | 攀枝花市农林科学研究院 | Ferfas is inoculated with bacterium ball and its preparation and application |
| CN107027519B (en) * | 2017-06-05 | 2019-06-25 | 攀枝花市农林科学研究院 | The mycorrhiza fungi seeding cultivating method of Panzhihua ferfas |
| CN107333566A (en) * | 2017-08-17 | 2017-11-10 | 佛山推启农业研究院（普通合伙） | A kind of method that russule is cultivated using Asiatic plantain Va Mycorrhiza Seedling |
| CN109937884A (en) * | 2019-04-26 | 2019-06-28 | 北京农学院 | A kind of method of Chinese chestnut and the in vitro symbiosis culture of fungi |
| CN109937884B (en) * | 2019-04-26 | 2021-10-01 | 北京农学院 | Method for in vitro symbiotic culture of Chinese chestnut and fungus |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105474992A (en) | Chinese chestnut summer truffle mycorrhizal seedling culture method based on symbiotic relation | |
| US10624278B2 (en) | Cultivation method for the rapid propagation of Davidia involucrata winter buds | |
| CN105638477B (en) | A kind of leaf of bamboo stem of noble dendrobium seed rapid propagation method | |
| CN106577281B (en) | The high planting percent breeding method of polygala arillata stem section tissue culture | |
| CN103070013A (en) | Method for manually inoculating and culturing root seedling of summer truffle | |
| CN103688865B (en) | Inducing medium and method for improving survival rate of butterfly orchid pedicel | |
| CN101116424B (en) | Highly effective lily bulblet inducement culture method | |
| CN107278851A (en) | The breeding method of Phyllostachys Pubescens Seedlings | |
| CN105993865A (en) | Cultivation method for quercus variabilis aseptic seedling | |
| CN106212281B (en) | A kind of method for tissue culture for improving banana survival rate | |
| CN105475067A (en) | Chinese chestnut tuber indicum mycorrhizal seedling culture method based on symbiotic relation | |
| CN108260532A (en) | A kind of seed tissue culture mating system of sealwort | |
| CN105165627B (en) | A kind of rosewood tissue culture sterilization formula and tissue culture method | |
| CN103931495A (en) | Tissue culture breeding method for dendrobium officinale | |
| CN104164472B (en) | A kind of indoor appraising method of eggplant Resistance to brown streak disease | |
| CN103070014B (en) | Method for breeding summer truffle root seedling through inoculation of suspension liquid | |
| CN105075858A (en) | Liquid rapid-propagation method for rhizoma bletillae seeds | |
| CN105917913A (en) | Method for breeding mycorrhizal seedlings by truffles and quercus acutissima | |
| CN106613960B (en) | A kind of Helen's pocket orchid callus regeneration system rapid propagation method | |
| CN109042330A (en) | A kind of method for tissue culture of spindle tree | |
| CN105684910B (en) | The tissue culture and rapid propagation method of dendrobium hancockii Rolfe | |
| CN103283604B (en) | Method for removing endophytic bacteria of explants in lily tissue culture | |
| CN109952869A (en) | Potato detoxicating micro potato breeding technique | |
| CN106069780B (en) | A technique for tissue culture breeding is carried out using bletilla stem tuber | |
| CN107372120A (en) | The cultural method that dove tree branch is quickly bred |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160413 |
|
| RJ01 | Rejection of invention patent application after publication |